Mthfd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfd1
Entrez Gene ID	108156
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Synonyms	Dcs,E430024A07Rik,Mthfd
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase Names C-1-tetrahydrofolate synthase, cytoplasmic C1-THF synthase C1-tetrahydrofolate synthase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C3
Summary	This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. [provided by RefSeq, Oct 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138745.2	NP_620084.2	C-1-tetrahydrofolate synthase, cytoplasmic
XM_030246509.1	XP_030102369.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Neurospora crassa	NCU03877	XP_956550.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Macaca mulatta (Rhesus monkey)	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

Low Dietary Folate Interacts with MTHFD1 Synthetase Deficiency in Mice, a Model for the R653Q Variant, to Increase Incidence of Developmental Delays and Defects.
Christensen KE, Bahous RH, Hou W, Deng L, Malysheva OV, Arning E, Bottiglieri T, Caudill MA, Jerome-Majewska LA, Rozen R
The Journal of nutrition148(4)501-509(2018 04)

Deletion of one allele of Mthfd1 (methylenetetrahydrofolate dehydrogenase 1) impairs learning in mice.
Pjetri E, Zeisel SH
Behavioural brain research33271-74(2017 08)

Murine MTHFD1-synthetase deficiency, a model for the human MTHFD1 R653Q polymorphism, decreases growth of colorectal tumors.
Lévesque N, Christensen KE, Van Der Kraak L, Best AF, Deng L, Caldwell D, MacFarlane AJ, Beauchemin N, Rozen R
Molecular carcinogenesis56(3)1030-1040(2017 03)

Moderate folic acid supplementation and MTHFD1-synthetase deficiency in mice, a model for the R653Q variant, result in embryonic defects and abnormal placental development.
Christensen KE, Hou W, Bahous RH, Deng L, Malysheva OV, Arning E, Bottiglieri T, Caudill MA, Jerome-Majewska LA, Rozen R
The American journal of clinical nutrition104(5)1459-1469(2016 11)

Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism.
MacFarlane AJ, Perry CA, Girnary HH, Gao D, Allen RH, Stabler SP, Shane B, Stover PJ
The Journal of biological chemistry284(3)1533-9(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mthfd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu85980	XM_030246509.1 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu18185	NM_138745.2 Latest version!	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$580.30 $829.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfd1 ( XM_030246509.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030102369.1 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu85980
Clone ID Related Accession (Same CDS sequence)	XM_030246509.1
Accession Version	XM_030246509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGACC TCCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCCACAGG CCAGCCTGAG 
ATGGTGAAGG GGGAGTGGAT CAAGCCTGGG GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTCGTGG GTGATGTGGC CTACGATGAG 
GCCAAGGAGA GGGCGAGCTT TATCACGCCT GTCCCTGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTACAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CTCCTGTCCC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCAAAGCTC ATCGGGAACC TGGCCCGAGA AATCGGGCTG 
CTCACCGAAG AGGTGGAGTT GTACGGGGAA ACGAAGGCTA AAGTCTTGCT GTCAGCATTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TATGTCGTGG TGACTGGAAT TACTCCAACA 
CCGCTGGGAG AAGGGAAGAG TACCACCACG ATCGGGCTGG TGCAGGCCCT CGGCGCCCAC 
CTGCGACAGA ACGTCTTTGC ATGTGTGCGA CAGCCTTCGC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAACCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCTGCTAACA ACCTCGTGGC TGCTGCCATT 
GATGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCTTCAGTAA ACGGAATAAG AAAATTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CACCACGCTG ACAGATGACG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ACCCAGAAAC CATAACATGG CAGAGAGTGT TGGACACCAA CGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGTCTCCA ACAGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAGATCATG GCAGTCCTGG CCCTCACGAG CTCTCTGGAA 
GACATGAGAG AGAGACTGGG CAGAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGTTGCGAAG ACCTGGGCGT GAGTGGGGCG CTGACGGTGC TGATGAAGGA TGCAATCAAG 
CCCAACCTCA TGCAGACCTT AGAGGGCACG CCAGTGTTTG TCCATGCCGG GCCTTTCGCC 
AACATTGCAC ACGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTAGGC 
CCTGAGGGCT TCGTAGTAAC GGAAGCTGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCACACG TGGTGGTTCT GGTTGCCACT 
GTCAGGGCCC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTTGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAAATCGAAA ATGCTAGAAT GTTTGGAGTC CCTGTCGTCG TGGCTGTGAA CGTGTTCAAG 
ACAGATACAG ATGCTGAGCT GGACCTTGTC AGCCGCCTCT CCAGAGAACA CGGGGCTTTT 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GGGCCTTGGC CCTGGCTCAG 
GCTGTCCAGA GAGCGTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAATTGAGG ATAAAATCAG GATTATTGCA CAGAGGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCGG AAGCACAGAA CAAAGCAGAA ATTTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGTT TCGTTCTGCC CATCCGGGAC ATCCGTGCCA GCGTTGGGGC AGGTTTCCTG 
TATCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACGCGGCC CTGTTTTTAT 
GATATTGATT TGGATCCTGA AACGGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030102369.1
CDS	1554..3821
Translation	MHDLLLWNNATVTTCHSKTANLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFSDIQIRRLRRLGIEKTDPTTLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_030246509.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246509.1

ORF Insert Sequence:

ATGCATGACC TCCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCCACAGG CCAGCCTGAG 
ATGGTGAAGG GGGAGTGGAT CAAGCCTGGG GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTCGTGG GTGATGTGGC CTACGATGAG 
GCCAAGGAGA GGGCGAGCTT TATCACGCCT GTCCCTGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTACAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CTCCTGTCCC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCAAAGCTC ATCGGGAACC TGGCCCGAGA AATCGGGCTG 
CTCACCGAAG AGGTGGAGTT GTACGGGGAA ACGAAGGCTA AAGTCTTGCT GTCAGCATTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TATGTCGTGG TGACTGGAAT TACTCCAACA 
CCGCTGGGAG AAGGGAAGAG TACCACCACG ATCGGGCTGG TGCAGGCCCT CGGCGCCCAC 
CTGCGACAGA ACGTCTTTGC ATGTGTGCGA CAGCCTTCGC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAACCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCTGCTAACA ACCTCGTGGC TGCTGCCATT 
GATGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCTTCAGTAA ACGGAATAAG AAAATTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CACCACGCTG ACAGATGACG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ACCCAGAAAC CATAACATGG CAGAGAGTGT TGGACACCAA CGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGTCTCCA ACAGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAGATCATG GCAGTCCTGG CCCTCACGAG CTCTCTGGAA 
GACATGAGAG AGAGACTGGG CAGAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGTTGCGAAG ACCTGGGCGT GAGTGGGGCG CTGACGGTGC TGATGAAGGA TGCAATCAAG 
CCCAACCTCA TGCAGACCTT AGAGGGCACG CCAGTGTTTG TCCATGCCGG GCCTTTCGCC 
AACATTGCAC ACGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTAGGC 
CCTGAGGGCT TCGTAGTAAC GGAAGCTGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCACACG TGGTGGTTCT GGTTGCCACT 
GTCAGGGCCC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTTGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAAATCGAAA ATGCTAGAAT GTTTGGAGTC CCTGTCGTCG TGGCTGTGAA CGTGTTCAAG 
ACAGATACAG ATGCTGAGCT GGACCTTGTC AGCCGCCTCT CCAGAGAACA CGGGGCTTTT 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GGGCCTTGGC CCTGGCTCAG 
GCTGTCCAGA GAGCGTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAATTGAGG ATAAAATCAG GATTATTGCA CAGAGGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCGG AAGCACAGAA CAAAGCAGAA ATTTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGTT TCGTTCTGCC CATCCGGGAC ATCCGTGCCA GCGTTGGGGC AGGTTTCCTG 
TATCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACGCGGCC CTGTTTTTAT 
GATATTGATT TGGATCCTGA AACGGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1 ( NM_138745.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_620084.2 Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), mRNA.

Price & Availability↑

CloneID	OMu18185
Clone ID Related Accession (Same CDS sequence)	NM_138745.2
Accession Version	NM_138745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Mus musculus(house mouse)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD545120.1, AK159812.1, AK042958.1 and AW536566.1. On Oct 23, 2009 this sequence version replaced NM_138745.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129012.1, SRR1660815.187204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCAG CGGGAATCCT GAACGGGAAA CTGGTTTCTG CGCAAATCAG GGATCGACTG 
AAGAACCAGG TAACTCGGAT GCAGGAGCAG GTACCTGGCT TCACCCCTGG CCTGGCAATC 
CTGCAGGTTG GCGACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAATTACCAA GAACATCCAC AGAGTCCGAG 
GTGTTAAAAT ATGTCATCTC CCTGAACGAG GATGCCTCTG TGCACGGATT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAGGCAG TCATCAATGC CATTGCACCC 
GAGAAGGATG TGGATGGGTT GACGAGCGTC AGTGCTGGGA AGCTCGCCAG AGGTGATCTA 
AATGACTGCT TCATCCCATG CACACCCAAG GGATGCTTGG AACTCATCAA AGAGGCAGGG 
GTGCAGATTG CCGGAAGGCA CGCGGTGGTG GTTGGTCGAA GTAAGATAGT TGGTGCACCC 
ATGCATGACC TCCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCCACAGG CCAGCCTGAG 
ATGGTGAAGG GGGAGTGGAT CAAGCCTGGG GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTCGTGG GTGATGTGGC CTACGATGAG 
GCCAAGGAGA GGGCGAGCTT TATCACGCCT GTCCCTGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTACAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CTCCTGTCCC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCAAAGCTC ATCGGGAACC TGGCCCGAGA AATCGGGCTG 
CTCACCGAAG AGGTGGAGTT GTACGGGGAA ACGAAGGCTA AAGTCTTGCT GTCAGCATTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TATGTCGTGG TGACTGGAAT TACTCCAACA 
CCGCTGGGAG AAGGGAAGAG TACCACCACG ATCGGGCTGG TGCAGGCCCT CGGCGCCCAC 
CTGCGACAGA ACGTCTTTGC ATGTGTGCGA CAGCCTTCGC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAACCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCTGCTAACA ACCTCGTGGC TGCTGCCATT 
GATGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCTTCAGTAA ACGGAATAAG AAAATTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CACCACGCTG ACAGATGACG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ACCCAGAAAC CATAACATGG CAGAGAGTGT TGGACACCAA CGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGTCTCCA ACAGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAGATCATG GCAGTCCTGG CCCTCACGAG CTCTCTGGAA 
GACATGAGAG AGAGACTGGG CAGAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGTTGCGAAG ACCTGGGCGT GAGTGGGGCG CTGACGGTGC TGATGAAGGA TGCAATCAAG 
CCCAACCTCA TGCAGACCTT AGAGGGCACG CCAGTGTTTG TCCATGCCGG GCCTTTCGCC 
AACATTGCAC ACGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTAGGC 
CCTGAGGGCT TCGTAGTAAC GGAAGCTGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCACACG TGGTGGTTCT GGTTGCCACT 
GTCAGGGCCC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTTGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAAATCGAAA ATGCTAGAAT GTTTGGAGTC CCTGTCGTCG TGGCTGTGAA CGTGTTCAAG 
ACAGATACAG ATGCTGAGCT GGACCTTGTC AGCCGCCTCT CCAGAGAACA CGGGGCTTTT 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GGGCCTTGGC CCTGGCTCAG 
GCTGTCCAGA GAGCGTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAATTGAGG ATAAAATCAG GATTATTGCA CAGAGGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCGG AAGCACAGAA CAAAGCAGAA ATTTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGTT TCGTTCTGCC CATCCGGGAC ATCCGTGCCA GCGTTGGGGC AGGTTTCCTG 
TATCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACGCGGCC CTGTTTTTAT 
GATATTGATT TGGATCCTGA AACGGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620084.2
CDS	273..3080
Translation	MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAEEIGIKATHIKLPRTSTESEVLKYVISLNEDASVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSVSAGKLARGDLNDCFIPCTPKGCLELIKEAGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFSDIQIRRLRRLGIEKTDPTTLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	NM_138745.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138745.2

ORF Insert Sequence:

ATGGCACCAG CGGGAATCCT GAACGGGAAA CTGGTTTCTG CGCAAATCAG GGATCGACTG 
AAGAACCAGG TAACTCGGAT GCAGGAGCAG GTACCTGGCT TCACCCCTGG CCTGGCAATC 
CTGCAGGTTG GCGACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAATTACCAA GAACATCCAC AGAGTCCGAG 
GTGTTAAAAT ATGTCATCTC CCTGAACGAG GATGCCTCTG TGCACGGATT CATAGTGCAG 
CTGCCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAGGCAG TCATCAATGC CATTGCACCC 
GAGAAGGATG TGGATGGGTT GACGAGCGTC AGTGCTGGGA AGCTCGCCAG AGGTGATCTA 
AATGACTGCT TCATCCCATG CACACCCAAG GGATGCTTGG AACTCATCAA AGAGGCAGGG 
GTGCAGATTG CCGGAAGGCA CGCGGTGGTG GTTGGTCGAA GTAAGATAGT TGGTGCACCC 
ATGCATGACC TCCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
AATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCCACAGG CCAGCCTGAG 
ATGGTGAAGG GGGAGTGGAT CAAGCCTGGG GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTCGTGG GTGATGTGGC CTACGATGAG 
GCCAAGGAGA GGGCGAGCTT TATCACGCCT GTCCCTGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTACAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CTCCTGTCCC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCAAAGCTC ATCGGGAACC TGGCCCGAGA AATCGGGCTG 
CTCACCGAAG AGGTGGAGTT GTACGGGGAA ACGAAGGCTA AAGTCTTGCT GTCAGCATTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TATGTCGTGG TGACTGGAAT TACTCCAACA 
CCGCTGGGAG AAGGGAAGAG TACCACCACG ATCGGGCTGG TGCAGGCCCT CGGCGCCCAC 
CTGCGACAGA ACGTCTTTGC ATGTGTGCGA CAGCCTTCGC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAACCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCTGCTAACA ACCTCGTGGC TGCTGCCATT 
GATGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCTTCAGTAA ACGGAATAAG AAAATTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CACCACGCTG ACAGATGACG AGATAAACAG ATTTGCAAGA 
CTGGATATTG ACCCAGAAAC CATAACATGG CAGAGAGTGT TGGACACCAA CGATAGATTC 
CTGAGGAAGA TCACAATTGG ACAGTCTCCA ACAGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAGATCATG GCAGTCCTGG CCCTCACGAG CTCTCTGGAA 
GACATGAGAG AGAGACTGGG CAGAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGTTGCGAAG ACCTGGGCGT GAGTGGGGCG CTGACGGTGC TGATGAAGGA TGCAATCAAG 
CCCAACCTCA TGCAGACCTT AGAGGGCACG CCAGTGTTTG TCCATGCCGG GCCTTTCGCC 
AACATTGCAC ACGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTAGGC 
CCTGAGGGCT TCGTAGTAAC GGAAGCTGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCACACG TGGTGGTTCT GGTTGCCACT 
GTCAGGGCCC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTTGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAAATCGAAA ATGCTAGAAT GTTTGGAGTC CCTGTCGTCG TGGCTGTGAA CGTGTTCAAG 
ACAGATACAG ATGCTGAGCT GGACCTTGTC AGCCGCCTCT CCAGAGAACA CGGGGCTTTT 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GGGCCTTGGC CCTGGCTCAG 
GCTGTCCAGA GAGCGTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAATTGAGG ATAAAATCAG GATTATTGCA CAGAGGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCGG AAGCACAGAA CAAAGCAGAA ATTTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCC TTGTCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGTT TCGTTCTGCC CATCCGGGAC ATCCGTGCCA GCGTTGGGGC AGGTTTCCTG 
TATCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACGCGGCC CTGTTTTTAT 
GATATTGATT TGGATCCTGA AACGGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Low Dietary Folate Interacts with MTHFD1 Synthetase Deficiency in Mice, a Model for the R653Q Variant, to Increase Incidence of Developmental Delays and Defects.
The Journal of nutrition148(4)501-509(2018 04)
Christensen KE,Bahous RH,Hou W,Deng L,Malysheva OV,Arning E,Bottiglieri T,Caudill MA,Jerome-Majewska LA,Rozen R

Deletion of one allele of Mthfd1 (methylenetetrahydrofolate dehydrogenase 1) impairs learning in mice.
Behavioural brain research33271-74(2017 08)
Pjetri E,Zeisel SH

Murine MTHFD1-synthetase deficiency, a model for the human MTHFD1 R653Q polymorphism, decreases growth of colorectal tumors.
Molecular carcinogenesis56(3)1030-1040(2017 03)
Lévesque N,Christensen KE,Van Der Kraak L,Best AF,Deng L,Caldwell D,MacFarlane AJ,Beauchemin N,Rozen R

Moderate folic acid supplementation and MTHFD1-synthetase deficiency in mice, a model for the R653Q variant, result in embryonic defects and abnormal placental development.
The American journal of clinical nutrition104(5)1459-1469(2016 11)
Christensen KE,Hou W,Bahous RH,Deng L,Malysheva OV,Arning E,Bottiglieri T,Caudill MA,Jerome-Majewska LA,Rozen R

Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism.
The Journal of biological chemistry284(3)1533-9(2009 Jan)
MacFarlane AJ,Perry CA,Girnary HH,Gao D,Allen RH,Stabler SP,Shane B,Stover PJ

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Mthfd1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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