Mthfd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfd1
Entrez Gene ID	64300
Full Name	methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 Names C-1-tetrahydrofolate synthase, cytoplasmic C1-THF synthase C1-tetrahydrofolate synthase methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	a trifunctional enzyme combining a 10-formyl-H4folate synthetase, a 5,10-methenyl-H4folate cyclohydrolase, and a 5,10-methylene-H4folate dehydrogenase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022508.1	NP_071953.1	C-1-tetrahydrofolate synthase, cytoplasmic
XM_006240249.3	XP_006240311.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Neurospora crassa	NCU03877	XP_956550.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Macaca mulatta (Rhesus monkey)	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology
Bibliography

Related articles in PubMed

Characterization and review of MTHFD1 deficiency: four new patients, cellular delineation and response to folic and folinic acid treatment.
Burda P, Kuster A, Hjalmarson O, Suormala T, Bürer C, Lutz S, Roussey G, Christa L, Asin-Cayuela J, Kollberg G, Andersson BA, Watkins D, Rosenblatt DS, Fowler B, Holme E, Froese DS, Baumgartner MR
Journal of inherited metabolic disease38(5)863-72(2015 Sep)

Folate metabolism gene polymorphisms and risk for down syndrome offspring in Turkish women.
Izci Ay O, Ay ME, Erdal ME, Cayan F, Tekin S, Soylemez F, Sungur MA, Derici Yıldırım D
Genetic testing and molecular biomarkers19(4)191-7(2015 Apr)

Association between MTHFD1 polymorphisms and neural tube defect susceptibility.
Meng J, Han L, Zhuang B
Journal of the neurological sciences348(1-2)188-94(2015 Jan)

Significant association of MTHFD1 1958G>A single nucleotide polymorphism with nonsyndromic cleft lip and palate in Indian population.
Murthy J, Gurramkonda VB, Lakkakula BV
Medicina oral, patologia oral y cirugia bucal19(6)e616-21(2014 Nov)

The role of 401a>G polymorphism of methylenetetrahydrofolate dehydrogenase gene (MTHFD1) in fetal hypotrophy.
Lorenc A, Seremak-Mrozikiewicz A, Barlik M, Wolski H, Drews K
Ginekologia polska85(7)494-9(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mthfd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05796	NM_022508.1 Latest version!	Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa29430	XM_006240249.3 Latest version!	Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfd1 ( NM_022508.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071953.1 Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), mRNA.

Price & Availability↑

CloneID	ORa05796
Clone ID Related Accession (Same CDS sequence)	NM_022508.1
Accession Version	NM_022508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05519.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05519.1, BC089800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCAG CGGGAATCCT GAACGGGAAA GTGGTCTCCG CGCAAATCAG GAATCGACTG 
AAGACCCAGG TTACTCAGAT GCAGGAGCAG GTACCTGGCT TCACCCCTGG CCTGGCAATC 
CTGCAGGTTG GCGACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
CAAGAGATCG GGATCAAAGC CACTCACATT AAATTACCAA GAACTTCCAC AGAGTCGGAG 
GTGTTAAAAT ACGTCATCTC CCTGAATGAG GATGCCACTG TGCACGGATT CATAGTACAG 
CTGCCTTTAG ACTCCGAGAA TTCCATTAAC ACAGAGGCAG TCATCAATGC CATTGCCCCT 
GAGAAGGATG TGGACGGGTT GACGAGCATC AATGCCGGGA AGCTTGCCAG AGGTGATCTA 
AAGGACTGCT TCATTCCGTG CACACCCAAA GGATGCTTGG AACTCATCAA AGAGACAGGA 
GTGCAGATTG CAGGAAGGCA CGCCGTGGTG GTCGGGCGAA GTAAAATAGT TGGTGCACCC 
ATGCACGACC TGCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
GATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCAACAGG ACAGCCTGAG 
ATGGTGAAAG GGGAGTGGAT CAAGCCTGGA GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTTGTGG GTGACGTAGC ATATGACGAG 
GCCAAGGAGA AGGCGAGCTT TATCACGCCT GTCCCCGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTGAAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CGCCTGTACC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCCAAGCTC ATCGGTAACC TGGCCCGAGA AATTGGGCTA 
CTCACTGAGG AGGTGGAATT GTATGGAGAA ACAAAGGCCA AGGTCTTACT GTCAGCACTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TACGTTGTGG TGACTGGAAT TACTCCAACA 
CCCCTGGGAG AAGGGAAGAG CACAACCACC ATCGGGCTTG TGCAAGCCCT TGGCGCCCAC 
CTGCATCAGA ATGTTTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCCGCTAATA ACCTTGTGGC TGCTGCTATT 
GACGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCCTCAGTAA ATGGAGTAAG AAAGTTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CGCTGCGCTG ACAGATGATG AGATAAACAG ATTTGCAAGA 
CTAGATATTG ATCCAGAAAC CATAACATGG CAGAGAGTGC TGGATACTAA CGATAGATTC 
CTGAGGAAGA TCACCATTGG ACAGGCTCCA ACCGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCAGTCCTGG CCCTCACTAG TTCTCTGGAA 
GACATGAGAG CAAGGCTGGG CAAAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGCTGTGAAG ACCTGGGCGT GAGCGGGGCG CTGACGGTGC TCATGAAGGA TGCGATCAAG 
CCCAATCTCA TGCAGACCTT AGAGGGCACG CCGGTATTTG TCCATGCTGG GCCTTTTGCC 
AACATTGCAC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTTGGC 
CCTGAGGGCT TTGTAGTGAC AGAAGCAGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCTCATG TGGTGGTTCT TGTTGCCACT 
GTCAGGGCTC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTGGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAGATAGAAA ATGCTAGAAT GTTTGGAGTG CCTGTCGTGG TGGCCATGAA TGCATTCAAG 
ACAGATACAG ATACTGAGCT GGACCTCATC GGCCGCCTCT CCAGAGAGCA TGGGGCTTTC 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GAGCCTTAGC CCTGGCTCAG 
GCTGTCCAGA GAGCTTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAGTTGAAG ATAAAATCAG GATTATTGCA CAGAAGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCTG AAGCACAAAA CAAAGCAGAA ATCTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCT TTATCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGCT TCGTTCTGCC CATCCGGGAC ATCCGGGCCA GCGTTGGGGC TGGTTTCCTG 
TACCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACTCGACC CTGTTTTTAT 
GATATCGATT TGGACCCTGA AACTGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071953.1
CDS	307..3114
Translation	MAPAGILNGKVVSAQIRNRLKTQVTQMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAQEIGIKATHIKLPRTSTESEVLKYVISLNEDATVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSINAGKLARGDLKDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTADLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKEKASFITPVPGGVGPMTVAMLMQSTVESAQRFLKKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPAALTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRARLGKMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAMNAFKTDTDTELDLIGRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSVEDKIRIIAQKIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	NM_022508.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022508.1

ORF Insert Sequence:

ATGGCACCAG CGGGAATCCT GAACGGGAAA GTGGTCTCCG CGCAAATCAG GAATCGACTG 
AAGACCCAGG TTACTCAGAT GCAGGAGCAG GTACCTGGCT TCACCCCTGG CCTGGCAATC 
CTGCAGGTTG GCGACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
CAAGAGATCG GGATCAAAGC CACTCACATT AAATTACCAA GAACTTCCAC AGAGTCGGAG 
GTGTTAAAAT ACGTCATCTC CCTGAATGAG GATGCCACTG TGCACGGATT CATAGTACAG 
CTGCCTTTAG ACTCCGAGAA TTCCATTAAC ACAGAGGCAG TCATCAATGC CATTGCCCCT 
GAGAAGGATG TGGACGGGTT GACGAGCATC AATGCCGGGA AGCTTGCCAG AGGTGATCTA 
AAGGACTGCT TCATTCCGTG CACACCCAAA GGATGCTTGG AACTCATCAA AGAGACAGGA 
GTGCAGATTG CAGGAAGGCA CGCCGTGGTG GTCGGGCGAA GTAAAATAGT TGGTGCACCC 
ATGCACGACC TGCTTCTGTG GAACAATGCC ACGGTGACCA CCTGCCACTC CAAGACTGCC 
GATCTGGACA AGGAGGTAAA TAAAGGAGAC ATTCTGGTGG TTGCAACAGG ACAGCCTGAG 
ATGGTGAAAG GGGAGTGGAT CAAGCCTGGA GCTGTGGTCA TAGACTGTGG GATCAATTAT 
GTTCCAGATG ATACAAAACC AAATGGAAGG AAAGTTGTGG GTGACGTAGC ATATGACGAG 
GCCAAGGAGA AGGCGAGCTT TATCACGCCT GTCCCCGGTG GCGTGGGGCC CATGACCGTG 
GCAATGCTGA TGCAGAGCAC AGTAGAGAGC GCACAGCGCT TCCTGAAGAA ATTTAAGCCA 
GGGAAGTGGA CAATTCAGTA TAACAAGCTG AACCTCAAGA CGCCTGTACC AAGTGACATT 
GCTATATCAC GATCTTGCAA ACCCAAGCTC ATCGGTAACC TGGCCCGAGA AATTGGGCTA 
CTCACTGAGG AGGTGGAATT GTATGGAGAA ACAAAGGCCA AGGTCTTACT GTCAGCACTA 
GATCGCCTGA AGCATCAGCC AGATGGGAAA TACGTTGTGG TGACTGGAAT TACTCCAACA 
CCCCTGGGAG AAGGGAAGAG CACAACCACC ATCGGGCTTG TGCAAGCCCT TGGCGCCCAC 
CTGCATCAGA ATGTTTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGGATA 
AAAGGTGGCG CTGCAGGAGG CGGCTATTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACTG GTGACATCCA CGCCATCACT GCCGCTAATA ACCTTGTGGC TGCTGCTATT 
GACGCTCGGA TATTTCATGA GCTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTCTGGTA 
CCCTCAGTAA ATGGAGTAAG AAAGTTCTCT GACATCCAAA TCCGCAGGTT ACGGAGGCTA 
GGCATTGAGA AGACTGACCC CGCTGCGCTG ACAGATGATG AGATAAACAG ATTTGCAAGA 
CTAGATATTG ATCCAGAAAC CATAACATGG CAGAGAGTGC TGGATACTAA CGATAGATTC 
CTGAGGAAGA TCACCATTGG ACAGGCTCCA ACCGAGAAAG GCCACACACG CACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATCATG GCAGTCCTGG CCCTCACTAG TTCTCTGGAA 
GACATGAGAG CAAGGCTGGG CAAAATGGTG GTAGCGTCCA GTAAGAAAGG GGAGCCTATC 
AGCTGTGAAG ACCTGGGCGT GAGCGGGGCG CTGACGGTGC TCATGAAGGA TGCGATCAAG 
CCCAATCTCA TGCAGACCTT AGAGGGCACG CCGGTATTTG TCCATGCTGG GCCTTTTGCC 
AACATTGCAC ATGGGAATTC CTCCATCATT GCAGACCGGA TTGCACTCAA GCTGGTTGGC 
CCTGAGGGCT TTGTAGTGAC AGAAGCAGGA TTCGGAGCAG ACATAGGAAT GGAAAAGTTC 
TTCAACATCA AGTGCCGGTA TTCTGGTCTC CAGCCTCATG TGGTGGTTCT TGTTGCCACT 
GTCAGGGCTC TTAAGATGCA CGGGGGTGGC CCCACGGTCA CCGCTGGACT GCCTCTTCCC 
AAGGCTTACA CAGAAGAGGA CCTGGACCTG GTGGAAAAGG GCTTCAGTAA CTTGAGGAAA 
CAGATAGAAA ATGCTAGAAT GTTTGGAGTG CCTGTCGTGG TGGCCATGAA TGCATTCAAG 
ACAGATACAG ATACTGAGCT GGACCTCATC GGCCGCCTCT CCAGAGAGCA TGGGGCTTTC 
GATGCTGTCA AGTGCACCCA CTGGGCAGAA GGGGGCCAGG GAGCCTTAGC CCTGGCTCAG 
GCTGTCCAGA GAGCTTCACA GGCCCCCAGC AGCTTCCAGC TCCTCTATGA CCTCAAGCTC 
TCAGTTGAAG ATAAAATCAG GATTATTGCA CAGAAGATCT ACGGGGCCGA TGACATCGAA 
TTGCTCCCTG AAGCACAAAA CAAAGCAGAA ATCTACACAA AGCAGGGCTT TGGGAATCTA 
CCCATCTGCA TGGCCAAAAC ACACTTGTCT TTATCTCACA ACCCAGAGCA AAAAGGGGTG 
CCTACTGGCT TCGTTCTGCC CATCCGGGAC ATCCGGGCCA GCGTTGGGGC TGGTTTCCTG 
TACCCTTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CTACTCGACC CTGTTTTTAT 
GATATCGATT TGGACCCTGA AACTGAACAA GTGAATGGAT TGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfd1 ( XM_006240249.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240311.1 Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29430
Clone ID Related Accession (Same CDS sequence)	XM_006240249.3
Accession Version	XM_006240249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAGAC CAGGGATGTG GAGACGGGCA GCTGCCAGAC AGCCTGTTCT TGTTTCACGG 
CAAATCAGGA ATCGACTGAA GACCCAGGTT ACTCAGATGC AGGAGCAGGT ACCTGGCTTC 
ACCCCTGGCC TGGCAATCCT GCAGGTTGGC GACAGAGATG ATTCCAATCT TTATATAAAT 
GTGAAGCTGA AGGCTGCTCA AGAGATCGGG ATCAAAGCCA CTCACATTAA ATTACCAAGA 
ACTTCCACAG AGTCGGAGGT GTTAAAATAC GTCATCTCCC TGAATGAGGA TGCCACTGTG 
CACGGATTCA TAGTACAGCT GCCTTTAGAC TCAGAGAATT CCATTAACAC AGAGGCAGTC 
ATCAATGCCA TTGCCCCTGA GAAGGATGTG GACGGGTTGA CGAGCATCAA TGCCGGGAAG 
CTTGCCAGAG GTGATCTAAA GGACTGCTTC ATTCCGTGCA CACCCAAAGG ATGCTTGGAA 
CTCATCAAAG AGACAGGAGT GCAGATTGCA GGAAGGCACG CCGTGGTGGT CGGGCGAAGT 
AAAATAGTTG GTGCACCCAT GCACGACCTG CTTCTGTGGA ACAATGCCAC GGTGACCACC 
TGCCACTCCA AGACTGCCGA TCTGGACAAG GAGGTAAATA AAGGAGACAT TCTGGTGGTT 
GCAACAGGAC AGCCTGAGAT GGTGAAAGGG GAGTGGATCA AGCCTGGAGC TGTGGTCATA 
GACTGTGGGA TCAATTATGT TCCAGATGAT ACAAAACCAA ATGGAAGGAA AGTTGTGGGT 
GACGTAGCAT ATGACGAGGC CAAGGAGAAG GCGAGCTTTA TCACGCCTGT CCCCGGTGGC 
GTGGGGCCCA TGACCGTGGC AATGCTGATG CAGAGCACAG TAGAGAGCGC ACAGCGCTTC 
CTGAAGAAAT TTAAGCCAGG GAAGTGGACA ATTCAGTATA ACAAGCTGAA CCTCAAGACG 
CCTGTACCAA GTGACATTGC TATATCACGA TCTTGCAAAC CCAAGCTCAT CGGTAACCTG 
GCCCGAGAAA TTGGGCTACT CACTGAGGAG GTGGAATTGT ATGGAGAAAC AAAGGCCAAG 
GTCTTACTGT CAGCACTAGA TCGCCTGAAG CATCAGCCAG ATGGGAAATA CGTTGTGGTG 
ACTGGAATTA CTCCAACACC CCTGGGAGAA GGGAAGAGCA CAACCACCAT CGGGCTTGTG 
CAAGCCCTCG GCGCCCACCT GCATCAGAAT GTTTTTGCGT GTGTGCGACA GCCTTCTCAG 
GGCCCCACCT TTGGGATAAA AGGTGGCGCT GCAGGAGGCG GCTATTCCCA GGTCATTCCT 
ATGGAAGAGT TTAATCTCCA CCTCACTGGT GACATCCACG CCATCACTGC CGCTAATAAC 
CTTGTGGCTG CTGCTATTGA CGCTCGGATA TTTCATGAGC TGACCCAGAC AGACAAGGCT 
CTCTTTAATC GTCTGGTACC CTCAGTAAAT GGAGTAAGAA AGTTCTCTGA CATCCAAATC 
CGCAGGTTAC GGAGGCTAGG CATTGAGAAG ACTGACCCCG CTGCGCTGAC AGATGATGAG 
ATAAACAGAT TTGCAAGACT AGATATTGAT CCAGAAACCA TAACATGGCA GAGAGTGCTG 
GATACTAACG ATAGATTCCT GAGGAAGATC ACCATTGGAC AGGCTCCAAC CGAGAAAGGC 
CACACACGCA CGGCCCAGTT TGATATCTCT GTGGCCAGTG AAATCATGGC AGTCCTGGCC 
CTCACTAGTT CTCTGGAAGA CATGAGAGCA AGGCTGGGCA AAATGGTGGT AGCGTCCAGT 
AAGAAAGGGG AGCCTATCAG CTGTGAAGAC CTGGGCGTGA GCGGGGCGCT GACGGTGCTC 
ATGAAGGATG CGATCAAGCC CAATCTCATG CAGACCTTAG AGGGCACGCC GGTATTTGTC 
CATGCTGGGC CTTTTGCCAA CATTGCACAT GGGAATTCCT CCATCATTGC AGACCGGATT 
GCACTCAAGC TGGTTGGCCC TGAGGGCTTT GTAGTGACAG AAGCAGGATT CGGAGCAGAC 
ATAGGAATGG AAAAGTTCTT CAACATCAAG TGCCGGTATT CTGGTCTCCA GCCTCATGTG 
GTGGTTCTTG TTGCCACTGT CAGGGCTCTT AAGATGCACG GGGGTGGCCC CACGGTCACC 
GCTGGACTGC CTCTTCCCAA GGCTTACACA GAAGAGGACC TGGACCTGGT GGAAAAGGGC 
TTCAGTAACT TGAGGAAACA GATAGAAAAT GCTAGAATGT TTGGAGTGCC TGTCGTGGTG 
GCCATGAATG CATTCAAGAC AGATACAGAT GCTGAGCTGG ACCTCATCGG CCGCCTCTCC 
AGAGAGCATG GGGCTTTCGA TGCTGTCAAG TGCACCCACT GGGCAGAAGG GGGCCAGGGA 
GCCTTAGCCC TGGCTCAGGC TGTCCAGAGA GCTTCACAGG CCCCCAGCAG CTTCCAGCTC 
CTCTATGACC TCAAGCTCTC AGTTGAAGAT AAAATCAGGA TTATTGCACA GAAGATCTAC 
GGGGCCGATG ACATCGAATT GCTCCCTGAA GCACAAAACA AAGCAGAAAT CTACACAAAG 
CAGGGCTTTG GGAATCTACC CATCTGCATG GCCAAAACAC ACTTGTCTTT ATCTCACAAC 
CCAGAGCAAA AAGGGGTGCC TACTGGCTTC GTTCTGCCCA TCCGGGACAT CCGGGCCAGC 
GTTGGGGCTG GTTTCCTGTA CCCTTTAGTA GGAACGATGA GCACAATGCC TGGACTCCCT 
ACTCGACCCT GTTTTTATGA TATCGATTTG GACCCTGAAA CTGAACAAGT GAATGGATTG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240311.1
CDS	425..3250
Translation	MWRPGMWRRAAARQPVLVSRQIRNRLKTQVTQMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAQEIGIKATHIKLPRTSTESEVLKYVISLNEDATVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSINAGKLARGDLKDCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTADLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKEKASFITPVPGGVGPMTVAMLMQSTVESAQRFLKKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLHQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPAALTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRARLGKMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAMNAFKTDTDAELDLIGRLSREHGAFDAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSVEDKIRIIAQKIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	XM_006240249.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (Mthfd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240249.3

ORF Insert Sequence:

ATGTGGAGAC CAGGGATGTG GAGACGGGCA GCTGCCAGAC AGCCTGTTCT TGTTTCACGG 
CAAATCAGGA ATCGACTGAA GACCCAGGTT ACTCAGATGC AGGAGCAGGT ACCTGGCTTC 
ACCCCTGGCC TGGCAATCCT GCAGGTTGGC GACAGAGATG ATTCCAATCT TTATATAAAT 
GTGAAGCTGA AGGCTGCTCA AGAGATCGGG ATCAAAGCCA CTCACATTAA ATTACCAAGA 
ACTTCCACAG AGTCGGAGGT GTTAAAATAC GTCATCTCCC TGAATGAGGA TGCCACTGTG 
CACGGATTCA TAGTACAGCT GCCTTTAGAC TCAGAGAATT CCATTAACAC AGAGGCAGTC 
ATCAATGCCA TTGCCCCTGA GAAGGATGTG GACGGGTTGA CGAGCATCAA TGCCGGGAAG 
CTTGCCAGAG GTGATCTAAA GGACTGCTTC ATTCCGTGCA CACCCAAAGG ATGCTTGGAA 
CTCATCAAAG AGACAGGAGT GCAGATTGCA GGAAGGCACG CCGTGGTGGT CGGGCGAAGT 
AAAATAGTTG GTGCACCCAT GCACGACCTG CTTCTGTGGA ACAATGCCAC GGTGACCACC 
TGCCACTCCA AGACTGCCGA TCTGGACAAG GAGGTAAATA AAGGAGACAT TCTGGTGGTT 
GCAACAGGAC AGCCTGAGAT GGTGAAAGGG GAGTGGATCA AGCCTGGAGC TGTGGTCATA 
GACTGTGGGA TCAATTATGT TCCAGATGAT ACAAAACCAA ATGGAAGGAA AGTTGTGGGT 
GACGTAGCAT ATGACGAGGC CAAGGAGAAG GCGAGCTTTA TCACGCCTGT CCCCGGTGGC 
GTGGGGCCCA TGACCGTGGC AATGCTGATG CAGAGCACAG TAGAGAGCGC ACAGCGCTTC 
CTGAAGAAAT TTAAGCCAGG GAAGTGGACA ATTCAGTATA ACAAGCTGAA CCTCAAGACG 
CCTGTACCAA GTGACATTGC TATATCACGA TCTTGCAAAC CCAAGCTCAT CGGTAACCTG 
GCCCGAGAAA TTGGGCTACT CACTGAGGAG GTGGAATTGT ATGGAGAAAC AAAGGCCAAG 
GTCTTACTGT CAGCACTAGA TCGCCTGAAG CATCAGCCAG ATGGGAAATA CGTTGTGGTG 
ACTGGAATTA CTCCAACACC CCTGGGAGAA GGGAAGAGCA CAACCACCAT CGGGCTTGTG 
CAAGCCCTCG GCGCCCACCT GCATCAGAAT GTTTTTGCGT GTGTGCGACA GCCTTCTCAG 
GGCCCCACCT TTGGGATAAA AGGTGGCGCT GCAGGAGGCG GCTATTCCCA GGTCATTCCT 
ATGGAAGAGT TTAATCTCCA CCTCACTGGT GACATCCACG CCATCACTGC CGCTAATAAC 
CTTGTGGCTG CTGCTATTGA CGCTCGGATA TTTCATGAGC TGACCCAGAC AGACAAGGCT 
CTCTTTAATC GTCTGGTACC CTCAGTAAAT GGAGTAAGAA AGTTCTCTGA CATCCAAATC 
CGCAGGTTAC GGAGGCTAGG CATTGAGAAG ACTGACCCCG CTGCGCTGAC AGATGATGAG 
ATAAACAGAT TTGCAAGACT AGATATTGAT CCAGAAACCA TAACATGGCA GAGAGTGCTG 
GATACTAACG ATAGATTCCT GAGGAAGATC ACCATTGGAC AGGCTCCAAC CGAGAAAGGC 
CACACACGCA CGGCCCAGTT TGATATCTCT GTGGCCAGTG AAATCATGGC AGTCCTGGCC 
CTCACTAGTT CTCTGGAAGA CATGAGAGCA AGGCTGGGCA AAATGGTGGT AGCGTCCAGT 
AAGAAAGGGG AGCCTATCAG CTGTGAAGAC CTGGGCGTGA GCGGGGCGCT GACGGTGCTC 
ATGAAGGATG CGATCAAGCC CAATCTCATG CAGACCTTAG AGGGCACGCC GGTATTTGTC 
CATGCTGGGC CTTTTGCCAA CATTGCACAT GGGAATTCCT CCATCATTGC AGACCGGATT 
GCACTCAAGC TGGTTGGCCC TGAGGGCTTT GTAGTGACAG AAGCAGGATT CGGAGCAGAC 
ATAGGAATGG AAAAGTTCTT CAACATCAAG TGCCGGTATT CTGGTCTCCA GCCTCATGTG 
GTGGTTCTTG TTGCCACTGT CAGGGCTCTT AAGATGCACG GGGGTGGCCC CACGGTCACC 
GCTGGACTGC CTCTTCCCAA GGCTTACACA GAAGAGGACC TGGACCTGGT GGAAAAGGGC 
TTCAGTAACT TGAGGAAACA GATAGAAAAT GCTAGAATGT TTGGAGTGCC TGTCGTGGTG 
GCCATGAATG CATTCAAGAC AGATACAGAT GCTGAGCTGG ACCTCATCGG CCGCCTCTCC 
AGAGAGCATG GGGCTTTCGA TGCTGTCAAG TGCACCCACT GGGCAGAAGG GGGCCAGGGA 
GCCTTAGCCC TGGCTCAGGC TGTCCAGAGA GCTTCACAGG CCCCCAGCAG CTTCCAGCTC 
CTCTATGACC TCAAGCTCTC AGTTGAAGAT AAAATCAGGA TTATTGCACA GAAGATCTAC 
GGGGCCGATG ACATCGAATT GCTCCCTGAA GCACAAAACA AAGCAGAAAT CTACACAAAG 
CAGGGCTTTG GGAATCTACC CATCTGCATG GCCAAAACAC ACTTGTCTTT ATCTCACAAC 
CCAGAGCAAA AAGGGGTGCC TACTGGCTTC GTTCTGCCCA TCCGGGACAT CCGGGCCAGC 
GTTGGGGCTG GTTTCCTGTA CCCTTTAGTA GGAACGATGA GCACAATGCC TGGACTCCCT 
ACTCGACCCT GTTTTTATGA TATCGATTTG GACCCTGAAA CTGAACAAGT GAATGGATTG 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization and review of MTHFD1 deficiency: four new patients, cellular delineation and response to folic and folinic acid treatment.
Journal of inherited metabolic disease38(5)863-72(2015 Sep)
Burda P,Kuster A,Hjalmarson O,Suormala T,Bürer C,Lutz S,Roussey G,Christa L,Asin-Cayuela J,Kollberg G,Andersson BA,Watkins D,Rosenblatt DS,Fowler B,Holme E,Froese DS,Baumgartner MR

Folate metabolism gene polymorphisms and risk for down syndrome offspring in Turkish women.
Genetic testing and molecular biomarkers19(4)191-7(2015 Apr)
Izci Ay O,Ay ME,Erdal ME,Cayan F,Tekin S,Soylemez F,Sungur MA,Derici Yıldırım D

Association between MTHFD1 polymorphisms and neural tube defect susceptibility.
Journal of the neurological sciences348(1-2)188-94(2015 Jan)
Meng J,Han L,Zhuang B

Significant association of MTHFD1 1958G>A single nucleotide polymorphism with nonsyndromic cleft lip and palate in Indian population.
Medicina oral, patologia oral y cirugia bucal19(6)e616-21(2014 Nov)
Murthy J,Gurramkonda VB,Lakkakula BV

The role of 401a>G polymorphism of methylenetetrahydrofolate dehydrogenase gene (MTHFD1) in fetal hypotrophy.
Ginekologia polska85(7)494-9(2014 Jul)
Lorenc A,Seremak-Mrozikiewicz A,Barlik M,Wolski H,Drews K

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Mthfd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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