TDRD12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TDRD12
Entrez Gene ID	100334979
Full Name	tudor domain containing 12
Synonyms	si:ch1073-415n3.1
General protein information	Preferred Names tudor domain containing 12 Names putative ATP-dependent RNA helicase TDRD12 tudor domain containing 12
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470710.1	XP_021326385.1	putative ATP-dependent RNA helicase TDRD12
XM_017354158.1	XP_017209647.1	putative ATP-dependent RNA helicase TDRD12

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	TDRD12	XP_001087212.2
Bos taurus (cattle)	TDRD12	XP_002695045.2
Xenopus tropicalis (tropical clawed frog)	tdrd12	NP_001072612.1
Danio rerio (zebrafish)	LOC100334979	XP_005163266.1
Mus musculus (house mouse)	Tdrd12	NP_082310.1
Rattus norvegicus (Norway rat)	Tdrd12	XP_006228963.1
Homo sapiens (human)	LOC101929327	XP_006723595.1
Canis lupus familiaris (dog)	TDRD12	XP_005616833.1
Gallus gallus (chicken)	TDRD12	XP_001232125.3

Gene Ontology

The following TDRD12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TDRD12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa141481	XM_021470710.1 Latest version!	Danio rerio tudor domain containing 12 (tdrd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa57867	XM_017354158.1 Latest version!	Danio rerio tudor domain containing 12 (tdrd12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TDRD12 ( XM_021470710.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326385.1 Danio rerio tudor domain containing 12 (tdrd12), mRNA.

Price & Availability↑

CloneID	ODa141481
Clone ID Related Accession (Same CDS sequence)	XM_021470710.1
Accession Version	XM_021470710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTGGAAA TACAAATAAT CAAGATCGAG GACCCGGGCT GTCTGTGGGC TCGAGTTCTG 
AAGGGTCCAG GCCTGCAGGT GGTCAGTCCA GAGGAATACG AGAACCTGAA GGTCAGAATG 
AACCTTTTCT ATCATGAGGT CGACCTGGAT GTTCAGAAAC TGAAGCCTTC GGAGCTGAAA 
GAGGGACTGG TGTGTGTGGT GTTCAGCGTG GCCCTGAAGA GCTGGTGTCG AGCTATTGTT 
GAGTCCGTCC TGCAGGGGGT GCTGGACACT CAGGCCTGGT GTCTGTTAGT GGACTACGGA 
GACCGCATCA TCGTTAGGGA GGACGACGTC AGGACTCCGC TTGAGAAGTT CCTGCTGCTT 
CCCTTCCGTG TGCGGCGCTT CAGGCTGGCC GCCGTTCGTC CTATGACACT GCAGGTGCCG 
CTCTCTGAGG ACACGGCCGA GCTGGTGCCG TCTGCATCGT GGGACAGCTC CGCCACTAAA 
TACCTGCTCA ACCTGCTGCA AGTGTCGACT CTGGTTGAAG CCGTTCTCTG TGAAACACAA 
GACGACTGCT CCTCCATCGA GCTCTACCTG ACCATCAATA ATACCAAGAT CTGTGTGAAC 
CATGAGCTGG TGGCCAAGAA GTTTGCGTGT TTCGTGAGCG ATGCGGACGG CAGCAGAAGC 
ACAGATCGGG CTCCGGTCAG TCTGGCGTGG GATATTTACT CCAGTCCTCA TCAGCTGCTG 
GAGATGAAGG GCTGCTGCCC GCTCAGAGCT CCGCCCCCGG ACACGCCCAC CAGAAAGCAG 
CGCACAGACC ACACACACAC AGAAGCAGAG CAGCAGAAAG CAGAAGCAGC AGAGGAAATA 
CACACACCGT CTGTGGGGAA ACCTCAAGTG CAGAAGCTGC GCCGTAATCC TGGAACATAT 
ACTATCGGCA GAGGATGGAG GATCCGAGCT GAAGCCACTG ACCTCTTCAC TGCTCAGATT 
CACCACAGTA AATCACAGTA CATGAAGTTT ACCACACTTT TACAGCCAAA GCAGATTCCT 
GAATCTGTGG AAGAGAAGCA GAGCAGCCCT GGTCAAACCG ATGACAACAG CGAGGGTTCA 
CGTGTTTACA ATGCTATCGA CTCTGAGGAG CTGAAAGAGG CAGATCTCCC ACAAGAGCCC 
AGTCTGGATG ATCATCTGGC CTGTGCACGA CTTATGCAGT TTCTGAACCC AGATCCACTC 
AATCCAGACC AGGAAACTGT TGATGCCAAA GTTGCGCGCT GTGATCCGGG TCACTCTGGT 
GTTTTGGTTC ACTCCGCCAT TCGCATTGAA CCCTGCAGTA ACCTGACGCG CGCCCCAATC 
ACAGAGCAGT TCAGAAAGTT CCTGTTGTGG AGGAAGTACA GTGGGCCGAA TGTGGCGGAG 
AGCTACTGTT GGCCTGCTGT GGCTCGGGGT TTTGACACGG TGCTGGTTTC TCACAGCGGG 
GACAATCCTC TGTCTTATAT TCCTCCCCTG CTGATGATCT TACAGACGGT TTCTGTGGTC 
TCCAGCCTCG CCGCTCGCAC AGGGCCGGTA GCCGTAATCC TCTGTCCAGG GTGGGAGAAG 
GTTCAGACGG TGCTGGAGCT CCTGGAGGAC AGTAAAGCCA CACACGGTCT CCATCCCACC 
AGTGTGCTGC TGGGTGTGGA CCAGGACGAG CCCAAGAAGT TCAAGATCCA GAAGAACTGT 
CAGCTGCTGG TCACGACCCC ATTCTCTCTG CTCCGGCTGC TGGAGGCTCA GTGTTTCCTC 
TTCCTGCGTC TCTGTCACCT GGTTCTGGAT GAGGCGGATC TGCTGTACTC GCAGGCTCCA 
GATCAGATCT CCATCATCCT GAAACACTTC CAGCAGCTGG TGTGTGCAGA GGAGCGGGTG 
GCGTGCCCAC GGCAGATCAT CGCTGTGGGT GGACGGTGGA GCGCGGGCAT CGACGGGCTG 
GTGAGGGAAC ACATGATCCA CCCGACCGTC ATCATCACTG TCATGGAGGA GGCAGCGCTG 
TATGGCAGAG TTCACCAGAG GGTGCAGTTG TGTCTGGATT GCGATAAAGT CTCGGTTCTT 
CTCTCGTCGC TGGATTTCCA GCCGCCAAAA CCGCAGAAAA CTCTGATCAT CACAAATTCT 
GCAGAGGAAA CGGAGCACGT GTATAAGGCG GTGGTAAACA CTGCGGTCTT CACACTCAAA 
GCTCACGAGG ACGCGACGGA CCAGTTCGAC TTCATCATTG ATCAGTGGAG GAAGGACATC 
AGCCAGGGAA CGCAAGTCAT TCTGGTGACC ACCAATGACT GCCTGAAGGC CCTCGGCATC 
CGGGATGCCA CCTGTGTGGT GCATTATGGG TTTCCCAGCA CCCCGAAGCT GTTCGGCAGC 
CGCCTGTTCT GCATGTCGGA GAATTTCAGG AAGCTGTCAG ACATGGATCA CAGTGAGGAC 
TGGACCCCCG GAGCTCAGTC TGTGCTGCTG CTGTCGGAGC AGAACGTCCG TCATGTGTGC 
GGTGTCCTGC GGTACCTGCG GAGAACCGGT GCTCTGCTGC CTCCGGAGCT GCTGCAGTTC 
GCTTTGGGCA TCCAGCAGGC CAAGGAGGAG CAGAAGACGC ACCGGCCGCT GTGCTGCTCG 
CTCAAGAGCT TCGGCTTCTG CAGAGACAGC ACAGTTTGTC CAGACAGACA CACAATCAAC 
ACGTCTCTGG ATCAGACGCA ACTTCCCGAC TCCGGGATGA TCATGGTTCT GCCGCTGTTC 
ATCAAGACGG CGAGTGTGTA CTGCGGGCGT ATCGTCAGAC AGAAGGAGGA TTTATATGAG 
GATCTGGCAA CCCAGATGAA ACTGCATTAT GCCAGTGAGA GACGCTGCGC TGTGGAGGTG 
CTGAAGGGGG CGCTGTATGC CATCCAGGAG GAGAACTGCT ACCACCGTGT GCGTGTGCTG 
GAGGTGCCGG AGAAAGGAGA GCAGCTGTTC AGCAGCGTCA CCGCCTGCCT CATAGACGAG 
GGCCGCACAC AGGAAGTGAA GTCACACCAG CTGCTCCAGC TTCCCGCTCA GTTCCAGGAT 
CTGCCCGGCC AGGCGGTGGA GATCATCCTG TGCAGAGTTC AGCCTGTGGA CGGAGAGGTG 
GACTGGAACC CGAAGGTGAC CAGAGCCGTC AGTTTGAAGA TCAGAGGGAA GCTTCATCAG 
GCGCGTGTTG TCATGAGTTT GGGAAACACC ATCTGGGTCG ACCCCATGGT ACGTATTCGT 
CATCACGGCG CCACCAACAG GCCGAGGTTT GCCGGTTTTG TGAGGTTTCT GTTCCCTCTC 
TGTGGTCTTC AGGTAAGAAT GGTGAGAGTG CCAGGACTGA AGACCTTCAT CAACGAGTAC 
AACGTCCACA CGGAGATCCT CGCCTCAGGC TTCGGGGTCA GTAATCCTCA GCATGTGGAT 
CTGCTGAAGA CGGTTTTGCA GAGAGACCAG AACCACAACA TGCTGGATCA GCAGCTCTCA 
GAACACAGGC TAGTCTATTC GCTTCATACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326385.1
CDS	72..3524
Translation	MLEIQIIKIEDPGCLWARVLKGPGLQVVSPEEYENLKVRMNLFYHEVDLDVQKLKPSELKEGLVCVVFSVALKSWCRAIVESVLQGVLDTQAWCLLVDYGDRIIVREDDVRTPLEKFLLLPFRVRRFRLAAVRPMTLQVPLSEDTAELVPSASWDSSATKYLLNLLQVSTLVEAVLCETQDDCSSIELYLTINNTKICVNHELVAKKFACFVSDADGSRSTDRAPVSLAWDIYSSPHQLLEMKGCCPLRAPPPDTPTRKQRTDHTHTEAEQQKAEAAEEIHTPSVGKPQVQKLRRNPGTYTIGRGWRIRAEATDLFTAQIHHSKSQYMKFTTLLQPKQIPESVEEKQSSPGQTDDNSEGSRVYNAIDSEELKEADLPQEPSLDDHLACARLMQFLNPDPLNPDQETVDAKVARCDPGHSGVLVHSAIRIEPCSNLTRAPITEQFRKFLLWRKYSGPNVAESYCWPAVARGFDTVLVSHSGDNPLSYIPPLLMILQTVSVVSSLAARTGPVAVILCPGWEKVQTVLELLEDSKATHGLHPTSVLLGVDQDEPKKFKIQKNCQLLVTTPFSLLRLLEAQCFLFLRLCHLVLDEADLLYSQAPDQISIILKHFQQLVCAEERVACPRQIIAVGGRWSAGIDGLVREHMIHPTVIITVMEEAALYGRVHQRVQLCLDCDKVSVLLSSLDFQPPKPQKTLIITNSAEETEHVYKAVVNTAVFTLKAHEDATDQFDFIIDQWRKDISQGTQVILVTTNDCLKALGIRDATCVVHYGFPSTPKLFGSRLFCMSENFRKLSDMDHSEDWTPGAQSVLLLSEQNVRHVCGVLRYLRRTGALLPPELLQFALGIQQAKEEQKTHRPLCCSLKSFGFCRDSTVCPDRHTINTSLDQTQLPDSGMIMVLPLFIKTASVYCGRIVRQKEDLYEDLATQMKLHYASERRCAVEVLKGALYAIQEENCYHRVRVLEVPEKGEQLFSSVTACLIDEGRTQEVKSHQLLQLPAQFQDLPGQAVEIILCRVQPVDGEVDWNPKVTRAVSLKIRGKLHQARVVMSLGNTIWVDPMVRIRHHGATNRPRFAGFVRFLFPLCGLQVRMVRVPGLKTFINEYNVHTEILASGFGVSNPQHVDLLKTVLQRDQNHNMLDQQLSEHRLVYSLHT

Target ORF information:

RefSeq Version	XM_021470710.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tudor domain containing 12 (tdrd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470710.1

ORF Insert Sequence:

ATGCTGGAAA TACAAATAAT CAAGATCGAG GACCCGGGCT GTCTGTGGGC TCGAGTTCTG 
AAGGGTCCAG GCCTGCAGGT GGTCAGTCCA GAGGAATACG AGAACCTGAA GGTCAGAATG 
AACCTTTTCT ATCATGAGGT CGACCTGGAT GTTCAGAAAC TGAAGCCTTC GGAGCTGAAA 
GAGGGACTGG TGTGTGTGGT GTTCAGCGTG GCCCTGAAGA GCTGGTGTCG AGCTATTGTT 
GAGTCCGTCC TGCAGGGGGT GCTGGACACT CAGGCCTGGT GTCTGTTAGT GGACTACGGA 
GACCGCATCA TCGTTAGGGA GGACGACGTC AGGACTCCGC TTGAGAAGTT CCTGCTGCTT 
CCCTTCCGTG TGCGGCGCTT CAGGCTGGCC GCCGTTCGTC CTATGACACT GCAGGTGCCG 
CTCTCTGAGG ACACGGCCGA GCTGGTGCCG TCTGCATCGT GGGACAGCTC CGCCACTAAA 
TACCTGCTCA ACCTGCTGCA AGTGTCGACT CTGGTTGAAG CCGTTCTCTG TGAAACACAA 
GACGACTGCT CCTCCATCGA GCTCTACCTG ACCATCAATA ATACCAAGAT CTGTGTGAAC 
CATGAGCTGG TGGCCAAGAA GTTTGCGTGT TTCGTGAGCG ATGCGGACGG CAGCAGAAGC 
ACAGATCGGG CTCCGGTCAG TCTGGCGTGG GATATTTACT CCAGTCCTCA TCAGCTGCTG 
GAGATGAAGG GCTGCTGCCC GCTCAGAGCT CCGCCCCCGG ACACGCCCAC CAGAAAGCAG 
CGCACAGACC ACACACACAC AGAAGCAGAG CAGCAGAAAG CAGAAGCAGC AGAGGAAATA 
CACACACCGT CTGTGGGGAA ACCTCAAGTG CAGAAGCTGC GCCGTAATCC TGGAACATAT 
ACTATCGGCA GAGGATGGAG GATCCGAGCT GAAGCCACTG ACCTCTTCAC TGCTCAGATT 
CACCACAGTA AATCACAGTA CATGAAGTTT ACCACACTTT TACAGCCAAA GCAGATTCCT 
GAATCTGTGG AAGAGAAGCA GAGCAGCCCT GGTCAAACCG ATGACAACAG CGAGGGTTCA 
CGTGTTTACA ATGCTATCGA CTCTGAGGAG CTGAAAGAGG CAGATCTCCC ACAAGAGCCC 
AGTCTGGATG ATCATCTGGC CTGTGCACGA CTTATGCAGT TTCTGAACCC AGATCCACTC 
AATCCAGACC AGGAAACTGT TGATGCCAAA GTTGCGCGCT GTGATCCGGG TCACTCTGGT 
GTTTTGGTTC ACTCCGCCAT TCGCATTGAA CCCTGCAGTA ACCTGACGCG CGCCCCAATC 
ACAGAGCAGT TCAGAAAGTT CCTGTTGTGG AGGAAGTACA GTGGGCCGAA TGTGGCGGAG 
AGCTACTGTT GGCCTGCTGT GGCTCGGGGT TTTGACACGG TGCTGGTTTC TCACAGCGGG 
GACAATCCTC TGTCTTATAT TCCTCCCCTG CTGATGATCT TACAGACGGT TTCTGTGGTC 
TCCAGCCTCG CCGCTCGCAC AGGGCCGGTA GCCGTAATCC TCTGTCCAGG GTGGGAGAAG 
GTTCAGACGG TGCTGGAGCT CCTGGAGGAC AGTAAAGCCA CACACGGTCT CCATCCCACC 
AGTGTGCTGC TGGGTGTGGA CCAGGACGAG CCCAAGAAGT TCAAGATCCA GAAGAACTGT 
CAGCTGCTGG TCACGACCCC ATTCTCTCTG CTCCGGCTGC TGGAGGCTCA GTGTTTCCTC 
TTCCTGCGTC TCTGTCACCT GGTTCTGGAT GAGGCGGATC TGCTGTACTC GCAGGCTCCA 
GATCAGATCT CCATCATCCT GAAACACTTC CAGCAGCTGG TGTGTGCAGA GGAGCGGGTG 
GCGTGCCCAC GGCAGATCAT CGCTGTGGGT GGACGGTGGA GCGCGGGCAT CGACGGGCTG 
GTGAGGGAAC ACATGATCCA CCCGACCGTC ATCATCACTG TCATGGAGGA GGCAGCGCTG 
TATGGCAGAG TTCACCAGAG GGTGCAGTTG TGTCTGGATT GCGATAAAGT CTCGGTTCTT 
CTCTCGTCGC TGGATTTCCA GCCGCCAAAA CCGCAGAAAA CTCTGATCAT CACAAATTCT 
GCAGAGGAAA CGGAGCACGT GTATAAGGCG GTGGTAAACA CTGCGGTCTT CACACTCAAA 
GCTCACGAGG ACGCGACGGA CCAGTTCGAC TTCATCATTG ATCAGTGGAG GAAGGACATC 
AGCCAGGGAA CGCAAGTCAT TCTGGTGACC ACCAATGACT GCCTGAAGGC CCTCGGCATC 
CGGGATGCCA CCTGTGTGGT GCATTATGGG TTTCCCAGCA CCCCGAAGCT GTTCGGCAGC 
CGCCTGTTCT GCATGTCGGA GAATTTCAGG AAGCTGTCAG ACATGGATCA CAGTGAGGAC 
TGGACCCCCG GAGCTCAGTC TGTGCTGCTG CTGTCGGAGC AGAACGTCCG TCATGTGTGC 
GGTGTCCTGC GGTACCTGCG GAGAACCGGT GCTCTGCTGC CTCCGGAGCT GCTGCAGTTC 
GCTTTGGGCA TCCAGCAGGC CAAGGAGGAG CAGAAGACGC ACCGGCCGCT GTGCTGCTCG 
CTCAAGAGCT TCGGCTTCTG CAGAGACAGC ACAGTTTGTC CAGACAGACA CACAATCAAC 
ACGTCTCTGG ATCAGACGCA ACTTCCCGAC TCCGGGATGA TCATGGTTCT GCCGCTGTTC 
ATCAAGACGG CGAGTGTGTA CTGCGGGCGT ATCGTCAGAC AGAAGGAGGA TTTATATGAG 
GATCTGGCAA CCCAGATGAA ACTGCATTAT GCCAGTGAGA GACGCTGCGC TGTGGAGGTG 
CTGAAGGGGG CGCTGTATGC CATCCAGGAG GAGAACTGCT ACCACCGTGT GCGTGTGCTG 
GAGGTGCCGG AGAAAGGAGA GCAGCTGTTC AGCAGCGTCA CCGCCTGCCT CATAGACGAG 
GGCCGCACAC AGGAAGTGAA GTCACACCAG CTGCTCCAGC TTCCCGCTCA GTTCCAGGAT 
CTGCCCGGCC AGGCGGTGGA GATCATCCTG TGCAGAGTTC AGCCTGTGGA CGGAGAGGTG 
GACTGGAACC CGAAGGTGAC CAGAGCCGTC AGTTTGAAGA TCAGAGGGAA GCTTCATCAG 
GCGCGTGTTG TCATGAGTTT GGGAAACACC ATCTGGGTCG ACCCCATGGT ACGTATTCGT 
CATCACGGCG CCACCAACAG GCCGAGGTTT GCCGGTTTTG TGAGGTTTCT GTTCCCTCTC 
TGTGGTCTTC AGGTAAGAAT GGTGAGAGTG CCAGGACTGA AGACCTTCAT CAACGAGTAC 
AACGTCCACA CGGAGATCCT CGCCTCAGGC TTCGGGGTCA GTAATCCTCA GCATGTGGAT 
CTGCTGAAGA CGGTTTTGCA GAGAGACCAG AACCACAACA TGCTGGATCA GCAGCTCTCA 
GAACACAGGC TAGTCTATTC GCTTCATACT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TDRD12 ( XM_017354158.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209647.1 Danio rerio tudor domain containing 12 (tdrd12), mRNA.

Price & Availability↑

CloneID	ODa57867
Clone ID Related Accession (Same CDS sequence)	XM_017354158.1
Accession Version	XM_017354158.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	putative ATP-dependent RNA helicase TDRD12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCTGGAAA TACAAATAAT CAAGATCGAG GACCCGGGCT GTCTGTGGGC TCGAGTTCTG 
AAGGGTCCAG GCCTGCAGGT GGTCAGTCCA GAGGAATACG AGAACCTGAA GGTCAGAATG 
AACCTTTTCT ATCATGAGGT CGACCTGGAT GTTCAGAAAC TGAAGCCTTC GGAGCTGAAA 
GAGGGACTGG TGTGTGTGGT GTTCAGCGTG GCCCTGAAAA GCTGGTGTCG AGCTGTGGTT 
GAGTCCGTCC TGCAGGGGGC GCTGGACACT CAGGCCTGGT GTCTGTTAGT GGACTATGGA 
GACCGCATCA TTGTCAGGGA GGACGACGTC AGGACTCCGC TTGAGAAGTT CCTGCTGCTT 
CCCTTCCGTG TGCGGCGCTT CAGGCTGGCC GCCGTTCGTC CTATGACACT GCAGGTGCCG 
CTCTCTGAGG ACACGGCCGA GCTGGTGCCG TCTGCATCGT GGGACAGCTC CGCCACTAAA 
TACCTGCTCA ACCTGCTGCA AGTGTCGACT CTGGTTGAAG CCGTTCTCTG TGAAACACAA 
GACGACTGCT CCGCCATCGA GCTCTACCTG ACCATCAATA ATACCAAGAT CTGTGTGAAC 
CATGAGCTGG TGGCCAAGAA GTTTGCGTGT TTCGTGAGCG ATGCGGATGG CAGCAGAAGC 
ACAGATCGGG CTCCGGTCAG TCTGGCGTGG GATATTTACT CCAGTCCTCA TCAGCTGCTG 
GAGATGAAGG GCTGCTGCCC GCTCAGAGCT CCGCCCCCGG ACACGCCCAC CAGAAAGCAG 
CGCACAGACC ACACACACAC AGAAGCAGAG CAGCAGAAAG CAGAAGCAGC AGAGGAAATA 
CACACACCGT CTGTGGGGAA ACCTCAAGTG CAGAAGCTGC GCCGTAATCC TGGAACATAT 
ACTATCGGCA GAGGATGGAG GATCCGAGCT GAAGCCACTG ACCTCTTCAC TGCTCAGATT 
CACCACAGTA AATCACAGTA CATGAAGTTT ACCACACTTT TACAGCCAAA GCAGATTCCT 
GAATCTGTGG AAGAGAAGCA GAGCAGCCCT GGTCAAACCG ATGACAACAG CGAGGGTTCA 
CGTGTTTACA ATGCTATCGA CTCTGAGGAG CTGAAAGAGG CAGATCTCCC ACAAGAGCCC 
AGTCTGGATG ATCATCTGGC CTGTGCACGA CTTATGCAGT TTCTGAACCC AGATCCACTC 
AATCCAGACC AGGAAACTGT TGATGCCAAA GTTGCGCGCT GTGATCCGGG TCACTCTGGT 
GTTTTGGTTC ACTCCGCCAT TCGCATTGAA CCCTGCAGTA ACCTGACGCG CGCCCCAATC 
ACAGAGCAGT TCAGAAAGTT CCTGTTGTGG AGGAAGTACA GTGGGCCGAA TGTGGCGGAG 
AGCTACTGTT GGCCTGCTGT GGCTCGGGGT TTTGACACGG TGCTGGTTTC TCACAGCGGG 
GACAATCCTC TGTCTTATAT TCCTCCCCTG CTGATGATCT TACAGACGGT TTCTGTGGTC 
TCCAGCCTCG CCGCTCGCAC AGGGCCGGTA GCCGTAATCC TCTGTCCAGG GTGGGAGAAG 
GTTCAGACGG TGCTGGAGCT CCTGGAGGAC AGTAAAGCCA CACACGGTCT CCATCCCACC 
AGTGTGCTGC TGGGTGTGGA CCAGGACGAG CCCAAGAAGT TCAAGATCCA GAAGAACTGT 
CAGCTGCTGG TCACGACCCC ATTCTCTCTG CTCCGGCTGC TGGAGGCTCA GTGTTTCCTC 
TTCCTGCGTC TCTGTCACCT GGTTCTGGAT GAGGCGGATC TGCTGTACTC GCAGGCTCCA 
GATCAGATCT CCATCATCCT GAAACACTTC CAGCAGCTGG TGTGTGCAGA GGAGCGGGTG 
GCGTGCCCAC GGCAGATCAT CGCTGTGGGT GGACGGTGGA GCGCGGGCAT CGACGGGCTG 
GTGAGGGAAC ACATGATCCA CCCGACCGTC ATCATCACTG TCATGGAGGA GGCAGCGCTG 
TATGGCAGAG TTCACCAGAG GGTGCAGTTG TGTCTGGATT GCGATAAAGT CTCGGTTCTT 
CTCTCGTCGC TGGATTTCCA GCCGCCAAAA CCGCAGAAAA CTCTGATCAT CACAAATTCT 
GCAGAGGAAA CGGAGCACGT GTATAAGGCG GTGGTAAACA CTGCGGTCTT CACACTCAAA 
GCTCACGAGG ACGCGACGGA CCAGTTCGAC TTCATCATTG ATCAGTGGAG GAAGGACATC 
AGCCAGGGAA CGCAAGTCAT TCTGGTGACC ACCAATGACT GCCTGAAGGC CCTCGGCATC 
CGGGATGCCA CCTGTGTGGT GCATTATGGG TTTCCCAGCA CCCCGAAGCT GTTCGGCAGC 
CGCCTGTTCT GCATGTCGGA GAATTTCAGG AAGCTGTCAG ACATGGATCA CAGTGAGGAC 
TGGACCCCCG GAGCTCAGTC TGTGCTGCTG CTGTCGGAGC AGAACGTCCG TCATGTGTGC 
GGTGTCCTGC GGTACCTGCG GAGAACCGGT GCTCTGCTGC CTCCGGAGCT GCTGCAGTTC 
GCTTTGGGCA TCCAGCAGGC CAAGGAGGAG CAGAAGACGC ACCGGCCGCT GTGCTGCTCG 
CTCAAGAGCT TCGGCTTCTG CAGAGACAGC ACAGTTTGTC CAGACAGACA CACAATCAAC 
ACGTCTCTGG ATCAGACGCA ACTTCCCGAC TCCGGGATGA TCATGGTTCT GCCGCTGTTC 
ATCAAGACGG CGAGTGTGTA CTGCGGGCGT ATCGTCAGAC AGAAGGAGGA TTTATATGAG 
GATCTGGCAA CCCAGATGAA ACTGCATTAT GCCAGTGAGA GACGCTGCGC TGTGGAGGTG 
CTGAAGGGGG CGCTGTATGC CATCCAGGAG GAGAACTGCT ACCACCGTGT GCGTGTGCTG 
GAGGTGCCGG AGAAAGGAGA GCAGCTGTTC AGCAGCGTCA CCGCCTGCCT CATAGACGAG 
GGCCGCACAC AGGAAGTGAA GTCACACCAG CTGCTCCAGC TTCCCGCTCA GTTCCAGGAT 
CTGCCCGGCC AGGCGGTGGA GATCATCCTG TGCAGAGTTC AGCCTGTGGA CGGAGAGGTG 
GACTGGAACC CGAAGGTGAC CAGAGCCGTC AGTTTGAAGA TCAGAGGGAA GCTTCATCAG 
GCGCGTGTTG TCATGAGTTT GGGAAACACC ATCTGGGTCG ACCCCATGGT AAGAATGGTG 
AGAGTGCCAG GACTGAAGAC CTTCATCAAC GAGTACAACG TCCACACGGA GATCCTCGCC 
TCAGGCTTCG GGGTCAGTAA TCCTCAGCAT GTGGATCTGC TGAAGACGGT TTTGCAGAGA 
GACCAGAACC ACAACATGCT GGATCAGCAG CTCTCAGAAC ACAGGCTAGT CTATTCGCTT 
CATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209647.1
CDS	74..3442
Translation	MLEIQIIKIEDPGCLWARVLKGPGLQVVSPEEYENLKVRMNLFYHEVDLDVQKLKPSELKEGLVCVVFSVALKSWCRAVVESVLQGALDTQAWCLLVDYGDRIIVREDDVRTPLEKFLLLPFRVRRFRLAAVRPMTLQVPLSEDTAELVPSASWDSSATKYLLNLLQVSTLVEAVLCETQDDCSAIELYLTINNTKICVNHELVAKKFACFVSDADGSRSTDRAPVSLAWDIYSSPHQLLEMKGCCPLRAPPPDTPTRKQRTDHTHTEAEQQKAEAAEEIHTPSVGKPQVQKLRRNPGTYTIGRGWRIRAEATDLFTAQIHHSKSQYMKFTTLLQPKQIPESVEEKQSSPGQTDDNSEGSRVYNAIDSEELKEADLPQEPSLDDHLACARLMQFLNPDPLNPDQETVDAKVARCDPGHSGVLVHSAIRIEPCSNLTRAPITEQFRKFLLWRKYSGPNVAESYCWPAVARGFDTVLVSHSGDNPLSYIPPLLMILQTVSVVSSLAARTGPVAVILCPGWEKVQTVLELLEDSKATHGLHPTSVLLGVDQDEPKKFKIQKNCQLLVTTPFSLLRLLEAQCFLFLRLCHLVLDEADLLYSQAPDQISIILKHFQQLVCAEERVACPRQIIAVGGRWSAGIDGLVREHMIHPTVIITVMEEAALYGRVHQRVQLCLDCDKVSVLLSSLDFQPPKPQKTLIITNSAEETEHVYKAVVNTAVFTLKAHEDATDQFDFIIDQWRKDISQGTQVILVTTNDCLKALGIRDATCVVHYGFPSTPKLFGSRLFCMSENFRKLSDMDHSEDWTPGAQSVLLLSEQNVRHVCGVLRYLRRTGALLPPELLQFALGIQQAKEEQKTHRPLCCSLKSFGFCRDSTVCPDRHTINTSLDQTQLPDSGMIMVLPLFIKTASVYCGRIVRQKEDLYEDLATQMKLHYASERRCAVEVLKGALYAIQEENCYHRVRVLEVPEKGEQLFSSVTACLIDEGRTQEVKSHQLLQLPAQFQDLPGQAVEIILCRVQPVDGEVDWNPKVTRAVSLKIRGKLHQARVVMSLGNTIWVDPMVRMVRVPGLKTFINEYNVHTEILASGFGVSNPQHVDLLKTVLQRDQNHNMLDQQLSEHRLVYSLHT

Target ORF information:

RefSeq Version	XM_017354158.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tudor domain containing 12 (tdrd12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354158.1

ORF Insert Sequence:

ATGCTGGAAA TACAAATAAT CAAGATCGAG GACCCGGGCT GTCTGTGGGC TCGAGTTCTG 
AAGGGTCCAG GCCTGCAGGT GGTCAGTCCA GAGGAATACG AGAACCTGAA GGTCAGAATG 
AACCTTTTCT ATCATGAGGT CGACCTGGAT GTTCAGAAAC TGAAGCCTTC GGAGCTGAAA 
GAGGGACTGG TGTGTGTGGT GTTCAGCGTG GCCCTGAAAA GCTGGTGTCG AGCTGTGGTT 
GAGTCCGTCC TGCAGGGGGC GCTGGACACT CAGGCCTGGT GTCTGTTAGT GGACTATGGA 
GACCGCATCA TTGTCAGGGA GGACGACGTC AGGACTCCGC TTGAGAAGTT CCTGCTGCTT 
CCCTTCCGTG TGCGGCGCTT CAGGCTGGCC GCCGTTCGTC CTATGACACT GCAGGTGCCG 
CTCTCTGAGG ACACGGCCGA GCTGGTGCCG TCTGCATCGT GGGACAGCTC CGCCACTAAA 
TACCTGCTCA ACCTGCTGCA AGTGTCGACT CTGGTTGAAG CCGTTCTCTG TGAAACACAA 
GACGACTGCT CCGCCATCGA GCTCTACCTG ACCATCAATA ATACCAAGAT CTGTGTGAAC 
CATGAGCTGG TGGCCAAGAA GTTTGCGTGT TTCGTGAGCG ATGCGGATGG CAGCAGAAGC 
ACAGATCGGG CTCCGGTCAG TCTGGCGTGG GATATTTACT CCAGTCCTCA TCAGCTGCTG 
GAGATGAAGG GCTGCTGCCC GCTCAGAGCT CCGCCCCCGG ACACGCCCAC CAGAAAGCAG 
CGCACAGACC ACACACACAC AGAAGCAGAG CAGCAGAAAG CAGAAGCAGC AGAGGAAATA 
CACACACCGT CTGTGGGGAA ACCTCAAGTG CAGAAGCTGC GCCGTAATCC TGGAACATAT 
ACTATCGGCA GAGGATGGAG GATCCGAGCT GAAGCCACTG ACCTCTTCAC TGCTCAGATT 
CACCACAGTA AATCACAGTA CATGAAGTTT ACCACACTTT TACAGCCAAA GCAGATTCCT 
GAATCTGTGG AAGAGAAGCA GAGCAGCCCT GGTCAAACCG ATGACAACAG CGAGGGTTCA 
CGTGTTTACA ATGCTATCGA CTCTGAGGAG CTGAAAGAGG CAGATCTCCC ACAAGAGCCC 
AGTCTGGATG ATCATCTGGC CTGTGCACGA CTTATGCAGT TTCTGAACCC AGATCCACTC 
AATCCAGACC AGGAAACTGT TGATGCCAAA GTTGCGCGCT GTGATCCGGG TCACTCTGGT 
GTTTTGGTTC ACTCCGCCAT TCGCATTGAA CCCTGCAGTA ACCTGACGCG CGCCCCAATC 
ACAGAGCAGT TCAGAAAGTT CCTGTTGTGG AGGAAGTACA GTGGGCCGAA TGTGGCGGAG 
AGCTACTGTT GGCCTGCTGT GGCTCGGGGT TTTGACACGG TGCTGGTTTC TCACAGCGGG 
GACAATCCTC TGTCTTATAT TCCTCCCCTG CTGATGATCT TACAGACGGT TTCTGTGGTC 
TCCAGCCTCG CCGCTCGCAC AGGGCCGGTA GCCGTAATCC TCTGTCCAGG GTGGGAGAAG 
GTTCAGACGG TGCTGGAGCT CCTGGAGGAC AGTAAAGCCA CACACGGTCT CCATCCCACC 
AGTGTGCTGC TGGGTGTGGA CCAGGACGAG CCCAAGAAGT TCAAGATCCA GAAGAACTGT 
CAGCTGCTGG TCACGACCCC ATTCTCTCTG CTCCGGCTGC TGGAGGCTCA GTGTTTCCTC 
TTCCTGCGTC TCTGTCACCT GGTTCTGGAT GAGGCGGATC TGCTGTACTC GCAGGCTCCA 
GATCAGATCT CCATCATCCT GAAACACTTC CAGCAGCTGG TGTGTGCAGA GGAGCGGGTG 
GCGTGCCCAC GGCAGATCAT CGCTGTGGGT GGACGGTGGA GCGCGGGCAT CGACGGGCTG 
GTGAGGGAAC ACATGATCCA CCCGACCGTC ATCATCACTG TCATGGAGGA GGCAGCGCTG 
TATGGCAGAG TTCACCAGAG GGTGCAGTTG TGTCTGGATT GCGATAAAGT CTCGGTTCTT 
CTCTCGTCGC TGGATTTCCA GCCGCCAAAA CCGCAGAAAA CTCTGATCAT CACAAATTCT 
GCAGAGGAAA CGGAGCACGT GTATAAGGCG GTGGTAAACA CTGCGGTCTT CACACTCAAA 
GCTCACGAGG ACGCGACGGA CCAGTTCGAC TTCATCATTG ATCAGTGGAG GAAGGACATC 
AGCCAGGGAA CGCAAGTCAT TCTGGTGACC ACCAATGACT GCCTGAAGGC CCTCGGCATC 
CGGGATGCCA CCTGTGTGGT GCATTATGGG TTTCCCAGCA CCCCGAAGCT GTTCGGCAGC 
CGCCTGTTCT GCATGTCGGA GAATTTCAGG AAGCTGTCAG ACATGGATCA CAGTGAGGAC 
TGGACCCCCG GAGCTCAGTC TGTGCTGCTG CTGTCGGAGC AGAACGTCCG TCATGTGTGC 
GGTGTCCTGC GGTACCTGCG GAGAACCGGT GCTCTGCTGC CTCCGGAGCT GCTGCAGTTC 
GCTTTGGGCA TCCAGCAGGC CAAGGAGGAG CAGAAGACGC ACCGGCCGCT GTGCTGCTCG 
CTCAAGAGCT TCGGCTTCTG CAGAGACAGC ACAGTTTGTC CAGACAGACA CACAATCAAC 
ACGTCTCTGG ATCAGACGCA ACTTCCCGAC TCCGGGATGA TCATGGTTCT GCCGCTGTTC 
ATCAAGACGG CGAGTGTGTA CTGCGGGCGT ATCGTCAGAC AGAAGGAGGA TTTATATGAG 
GATCTGGCAA CCCAGATGAA ACTGCATTAT GCCAGTGAGA GACGCTGCGC TGTGGAGGTG 
CTGAAGGGGG CGCTGTATGC CATCCAGGAG GAGAACTGCT ACCACCGTGT GCGTGTGCTG 
GAGGTGCCGG AGAAAGGAGA GCAGCTGTTC AGCAGCGTCA CCGCCTGCCT CATAGACGAG 
GGCCGCACAC AGGAAGTGAA GTCACACCAG CTGCTCCAGC TTCCCGCTCA GTTCCAGGAT 
CTGCCCGGCC AGGCGGTGGA GATCATCCTG TGCAGAGTTC AGCCTGTGGA CGGAGAGGTG 
GACTGGAACC CGAAGGTGAC CAGAGCCGTC AGTTTGAAGA TCAGAGGGAA GCTTCATCAG 
GCGCGTGTTG TCATGAGTTT GGGAAACACC ATCTGGGTCG ACCCCATGGT AAGAATGGTG 
AGAGTGCCAG GACTGAAGAC CTTCATCAAC GAGTACAACG TCCACACGGA GATCCTCGCC 
TCAGGCTTCG GGGTCAGTAA TCCTCAGCAT GTGGATCTGC TGAAGACGGT TTTGCAGAGA 
GACCAGAACC ACAACATGCT GGATCAGCAG CTCTCAGAAC ACAGGCTAGT CTATTCGCTT 
CATACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TDRD12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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