SMCHD1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMCHD1
Entrez Gene ID	490535
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
General protein information	Preferred Names structural maintenance of chromosomes flexible hinge domain containing 1 Names structural maintenance of chromosomes flexible hinge domain-containing protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005623029.3	XP_005623086.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1
XM_005623031.2	XP_005623088.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
XM_005623029.2	XP_005623086.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
XM_005623030.2	XP_005623087.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCHD1	XP_005623086.1
Bos taurus (cattle)	SMCHD1	NP_001193329.1
Gallus gallus (chicken)	SMCHD1	XP_419144.4
Danio rerio (zebrafish)	LOC570784	XP_699397.5
Pan troglodytes (chimpanzee)	SMCHD1	XP_512045.2
Macaca mulatta (Rhesus monkey)	SMCHD1	XP_001086253.2
Xenopus tropicalis (tropical clawed frog)	smchd1	XP_002938954.1
Homo sapiens (human)	SMCHD1	NP_056110.2
Mus musculus (house mouse)	Smchd1	NP_083163.3
Rattus norvegicus (Norway rat)	Smchd1	XP_244261.6

Gene Ontology

The following SMCHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMCHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09912	XM_005623029.3 Latest version!	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe09913	XM_005623030.2 Latest version!	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe09914	XM_005623031.2 Latest version!	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCe09912	XM_005623029.2	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMCHD1 ( XM_005623029.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623086.1 Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

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CloneID	OCe09912
Clone ID Related Accession (Same CDS sequence)	XM_005623029.3 , XM_005623029.2
Accession Version	XM_005623029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGGAT TATAACCAAA TCAGATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623086.1
CDS	16..6057
Translation	MAAAGGGGGGGGGGGGGGGPSAGTGEDGGGDGCRTVYLFDRREKESELGDRVLQVEERSDYTRFRASVCQTLGISPEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKASDSVHITNDDERFLHHLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKHTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIEDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTSFKGVITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEVKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSNGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLMDPPFRVGVPFNIPLEFQDEFGHTSQLGNDIQPVFEASGLSLHYEEITKGPNCVIRGVTAKGLVNSCQGKNFNLKVILPGLKEDTQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTTQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDTMHNLIFQMYDEGEREIHITSTLADKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVVIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSHLKTTFQENTQSISVKGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKVLLIDWPELKESIPVINGRELQNPLIVQLCDQWDNPAPVSQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHMMHVRAIYNKNIIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWQLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPKIQPATPAVSNVRSVASRTLVRDLHLTITDDYNNHTGIDLVGTVVATIKGSNEEDTDTPLFIGKVRTLEFPFVKGSAEITNLVLAENSPGRDSTEYFIVFEPQLPALSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKNIIMYRSLFEASNQLLDEMKCRVEEAKLKEAQLQNELKTHNIDIPTTQQMPHIEALLKRKLSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLVLGEQIDLLQQYRTAVNKSNSVIEDLNRQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPAKVEMTDCPIPPKRMRREASRQNRIITKSDV

Target ORF information:

RefSeq Version	XM_005623029.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623029.3

ORF Insert Sequence:

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGGAT TATAACCAAA TCAGATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_005623029.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623086.1 Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

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CloneID	OCe09912
Clone ID Related Accession (Same CDS sequence)	XM_005623029.3 , XM_005623029.2
Accession Version	XM_005623029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGGAT TATAACCAAA TCAGATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623086.1
CDS	390..6431
Translation	MAAAGGGGGGGGGGGGGGGPSAGTGEDGGGDGCRTVYLFDRREKESELGDRVLQVEERSDYTRFRASVCQTLGISPEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKASDSVHITNDDERFLHHLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKHTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIEDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTSFKGVITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEVKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSNGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLMDPPFRVGVPFNIPLEFQDEFGHTSQLGNDIQPVFEASGLSLHYEEITKGPNCVIRGVTAKGLVNSCQGKNFNLKVILPGLKEDTQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTTQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDTMHNLIFQMYDEGEREIHITSTLADKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVVIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSHLKTTFQENTQSISVKGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKVLLIDWPELKESIPVINGRELQNPLIVQLCDQWDNPAPVSQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHMMHVRAIYNKNIIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWQLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPKIQPATPAVSNVRSVASRTLVRDLHLTITDDYNNHTGIDLVGTVVATIKGSNEEDTDTPLFIGKVRTLEFPFVKGSAEITNLVLAENSPGRDSTEYFIVFEPQLPALSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKNIIMYRSLFEASNQLLDEMKCRVEEAKLKEAQLQNELKTHNIDIPTTQQMPHIEALLKRKLSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLVLGEQIDLLQQYRTAVNKSNSVIEDLNRQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPAKVEMTDCPIPPKRMRREASRQNRIITKSDV

Target ORF information:

RefSeq Version	XM_005623029.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623029.2

ORF Insert Sequence:

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGGAT TATAACCAAA TCAGATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_005623030.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623087.1 Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

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CloneID	OCe09913
Clone ID Related Accession (Same CDS sequence)	XM_005623030.2
Accession Version	XM_005623030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGTAG TTTTGCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623087.1
CDS	390..6422
Translation	MAAAGGGGGGGGGGGGGGGPSAGTGEDGGGDGCRTVYLFDRREKESELGDRVLQVEERSDYTRFRASVCQTLGISPEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKASDSVHITNDDERFLHHLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKHTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIEDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTSFKGVITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEVKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSNGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLMDPPFRVGVPFNIPLEFQDEFGHTSQLGNDIQPVFEASGLSLHYEEITKGPNCVIRGVTAKGLVNSCQGKNFNLKVILPGLKEDTQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTTQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDTMHNLIFQMYDEGEREIHITSTLADKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVVIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSHLKTTFQENTQSISVKGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKVLLIDWPELKESIPVINGRELQNPLIVQLCDQWDNPAPVSQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHMMHVRAIYNKNIIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWQLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPKIQPATPAVSNVRSVASRTLVRDLHLTITDDYNNHTGIDLVGTVVATIKGSNEEDTDTPLFIGKVRTLEFPFVKGSAEITNLVLAENSPGRDSTEYFIVFEPQLPALSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKNIIMYRSLFEASNQLLDEMKCRVEEAKLKEAQLQNELKTHNIDIPTTQQMPHIEALLKRKLSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLVLGEQIDLLQQYRTAVNKSNSVIEDLNRQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPAKVEMTDCPIPPKRMRREASRQNSSFAE

Target ORF information:

RefSeq Version	XM_005623030.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623030.2

ORF Insert Sequence:

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGTAG TTTTGCAGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMCHD1 ( XM_005623031.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623088.1 Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.

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CloneID	OCe09914
Clone ID Related Accession (Same CDS sequence)	XM_005623031.2
Accession Version	XM_005623031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGTTT TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623088.1
CDS	390..6419
Translation	MAAAGGGGGGGGGGGGGGGPSAGTGEDGGGDGCRTVYLFDRREKESELGDRVLQVEERSDYTRFRASVCQTLGISPEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVNQLLLTATKERIDFLPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNTGIRRIQIKLLFDETQGKPAVAVIDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKASDSVHITNDDERFLHHLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFLLWTRQLAHIYHYYIHGPKGNEKHTSKEVGPFNNIDIEISMFEKGKAPKIVNLREIEDDMQTLYINTAADSFEFKAHVEGDGVVEGIIRYHPFLYDRETYPDDPCFPSKLKDEDDDDDCFILEKAARGKRPIFECFWNGRLIPYTSVEDFDWCTPPKKRGLAPIECYNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKYDKQIKFTSFKGVITRPDLPSKKQGPWATYSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVKFFLYGDHDGEVYATGGEVQIAMEPQALYDEVKTVPIAKLDRTVAEKAVKKYVEDEMARLPDRLSVTWPEGDELLPNEIRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHSSNGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRPLPSKAIKFSVKEGKPEKFSFGLMDPPFRVGVPFNIPLEFQDEFGHTSQLGNDIQPVFEASGLSLHYEEITKGPNCVIRGVTAKGLVNSCQGKNFNLKVILPGLKEDTQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVLDESDNVTTQPKLIVHCKFLGAPNLPVYVVDCSSSGTSILTGSAIQVQNIKKDQTLKAKIEIPSCKDVAPVEKTIKLLPSSHVARLQIFSVEGQKAIQIKHQDEVNWIAGDTMHNLIFQMYDEGEREIHITSTLADKIKVNWTPEINKEHLLQGLLPDVQVPTSVKDMRYCQVSFQDDHVSLESAFTVRPLPDEPKHLKCELKGGKTVQMGQELQGEIVVIVTDQYGNQIQAFSPSSLSALGIAGIGLDSSHLKTTFQENTQSISVKGIKFIPGPPGNKDLCFTWREFSDFIRVQLISGPPAKVLLIDWPELKESIPVINGRELQNPLIVQLCDQWDNPAPVSQVKISLIKANNLKLTPSNQQHKTDERGRANLGVFSVYAPRGEHMMHVRAIYNKNIIEGPIIKLMILPDPEKPIRLNVKYDKDASFLAGGIFTDFMITVISEDDSIIKNINPARISMKMWQLSNSGNRPPANAETFSCNKIKDNDKEDGCFYFRDKAIPNKVGTYCIQFGFMMDKANILNSEQIIVDVLPNQPVKLVPKIQPATPAVSNVRSVASRTLVRDLHLTITDDYNNHTGIDLVGTVVATIKGSNEEDTDTPLFIGKVRTLEFPFVKGSAEITNLVLAENSPGRDSTEYFIVFEPQLPALSRTLESYILPFMFYNDVKKQQQMAALTKEKDQLSKNIIMYRSLFEASNQLLDEMKCRVEEAKLKEAQLQNELKTHNIDIPTTQQMPHIEALLKRKLSEQEELRKKPRRSCTLPNYTKGSGDVLGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARRIYDETQGRQQVLPLDSIYKKTLPDWKRPLPHYRNGKLYFKPIGDPVFARDLLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPIPKQCLVLGEQIDLLQQYRTAVNKSNSVIEDLNRQLEYLHTPDMKKKKQELDEQEKNLKLIEQKLGMTPTRKCNDSLRHPAKVEMTDCPIPPKRMRREASRQNSFAE

Target ORF information:

RefSeq Version	XM_005623031.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623031.2

ORF Insert Sequence:

ATGGCAGCGG CCGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGG CGGCGGGCCC 
TCCGCGGGGA CTGGCGAGGA CGGCGGAGGC GACGGCTGCA GGACGGTGTA CTTGTTTGAC 
CGGCGCGAGA AGGAGTCCGA GCTCGGGGAC CGGGTGCTGC AGGTCGAGGA GCGCTCGGAC 
TACACAAGGT TCCGCGCCTC TGTGTGTCAG ACACTTGGCA TTTCACCTGA AGAGAAATTT 
GTTATTACAA CAACAAGTAG GAAAGAAATT ACCTGTGATA ATTTTGATGA CACTGTTAAA 
GATGGAGTCA CTCTATACCT GCTACAGTCA GTCAATCAGC TACTACTAAC AGCTACAAAA 
GAACGAATTG ACTTCCTACC TCACTATGAT ACACTGGTTA AAAGTGGCAT GTATGAATAT 
TATGCAAGTG AAGGACAAAA TCCTTTGCCA TTTGCTCTTG CAGAATTAAT TGACAATTCA 
TTGTCTGCTA CTTCTAGAAA CACTGGTATT AGAAGAATAC AGATCAAATT GCTTTTTGAT 
GAAACACAAG GAAAACCCGC TGTTGCAGTG ATAGATAATG GAAGAGGAAT GACCTCTAAA 
CAGCTTAACA ACTGGGCCGT GTATAGGTTG TCAAAATTCA CAAGGCAAGG TGACTTTGAA 
AGTGATCATT CAGGATATGT TCGTCCAGTA CCGGTGCCAC GCAGTTTAAA TAGTGATATT 
TCCTATTTTG GTGTTGGGGG CAAACAAGCT GTTTTCTTTG TTGGACAATC AGCCAGAATG 
ATAAGCAAAC CTGCAGATTC CCAAGATGTT CATGAACTTG TACTTTCTAA AGAAGATTTT 
GAGAAGAAGG AGAAAAATAA AGAGGCAATA TATAGTGGCT ATATCAGAAA CAGAAAGGCA 
TCTGATTCTG TTCACATTAC AAATGATGAT GAAAGATTTC TACATCATCT TATCGTAGAG 
GAGAAGGAAA AAGATAGCTT TACTGCTGTG GTTATCACAG GAGTACAACC AGAACACATA 
CAGTATTTGA AAAATTATTT CCTTCTTTGG ACAAGACAGT TAGCGCATAT TTATCATTAC 
TATATTCATG GCCCAAAAGG AAATGAAAAA CATACATCAA AAGAAGTTGG ACCTTTCAAC 
AACATTGATA TTGAAATTTC TATGTTTGAA AAGGGGAAGG CACCTAAGAT TGTCAACCTA 
AGGGAAATAG AAGATGACAT GCAGACGTTG TATATAAACA CAGCAGCTGA TAGTTTTGAG 
TTCAAGGCTC ATGTTGAAGG AGATGGTGTA GTAGAAGGGA TTATTCGATA TCACCCTTTC 
TTATATGATA GAGAAACTTA CCCTGATGAT CCATGCTTTC CATCAAAATT AAAAGATGAA 
GATGATGATG ATGATTGTTT CATACTTGAG AAAGCTGCAA GAGGGAAAAG GCCTATTTTT 
GAATGTTTTT GGAATGGACG GCTAATACCA TATACATCTG TTGAGGACTT TGACTGGTGT 
ACTCCTCCAA AGAAGAGAGG GCTTGCACCA ATTGAATGTT ACAATAGAAT ATCTGGTGCA 
TTATTCACCA ATGACAAATT CCAGGTCAGC ACAAATAAAC TGACTTTTAT GGATCTTGAG 
CTAAAGTTGA AAGATAAGAA CACCCTTTTT ACAAGGATTT TAAATGGACA GGAGCAGCGA 
ATGAAAATTG ACAGAGAATT TGCTTTATGG TTAAAGGACT GTCATGAAAA ATATGACAAA 
CAAATAAAAT TTACATCTTT TAAAGGAGTA ATTACACGTC CTGATCTTCC TTCTAAAAAG 
CAGGGCCCCT GGGCAACATA CTCAGCAATA GAATGGGATG GAAAAATATA CAAAGCAGGA 
CAGTTGGTCA AGACAATCAA GACACTTCCT CTCTTTTATG GAAGCATAGT AAAATTTTTT 
CTTTATGGTG ATCATGATGG AGAAGTATAT GCCACAGGAG GAGAGGTTCA GATTGCAATG 
GAACCTCAGG CGCTATATGA TGAAGTAAAA ACTGTGCCAA TTGCTAAGCT TGATAGGACA 
GTTGCTGAGA AAGCTGTCAA AAAATATGTA GAAGATGAAA TGGCAAGACT CCCTGATAGA 
TTGTCAGTAA CTTGGCCTGA AGGAGATGAA TTATTGCCAA ATGAGATTAG GCCAGCTGGA 
ACCCCCATTG GTGCATTAAG AATTGAAATA CTGAATAAAA AGGGGGAAGC AATGCAAAAG 
CTTCCAGGAA CAAGTCATGG AGGATCAAAG AAACTCCTGG TTGAGCTCAA AGTTATATTA 
CATTCTTCCA ATGGAAATAA AGAAATCATT TCACATATTA GTCAACATGG AGGAAAATGG 
CCTTACTGGT TTAAAAAAAT GGAAAATATT CAAAAGTTGG GGAATTATAC ATTGAAATTA 
CAAGTTGTAT TGAATGAAAG TAATGCAGAC ACTTATGCAG GAAGACCACT ACCTTCAAAA 
GCAATTAAAT TTTCTGTTAA AGAGGGTAAA CCAGAAAAGT TTTCATTTGG TCTTATGGAT 
CCTCCTTTTC GTGTTGGAGT CCCATTCAAT ATCCCTCTGG AGTTTCAGGA TGAATTTGGT 
CATACTAGTC AATTAGGAAA TGATATTCAG CCAGTATTTG AAGCAAGTGG TTTATCTTTA 
CATTATGAAG AAATAACCAA AGGACCGAAT TGTGTGATTC GAGGTGTTAC AGCTAAGGGC 
CTTGTAAACT CTTGTCAAGG CAAGAATTTT AATTTGAAGG TTATTCTGCC GGGTTTAAAA 
GAAGACACAC AGATTTTAAA AATTAGATTA CTACCAGGTC CCCCTCATCG ATTGAAAGTG 
AAACCTGATT CTGAAATTTT AGTTATAGAA AATGGAACAG CTTTCCCATT TCAGGTAGAA 
GTTTTGGATG AATCAGACAA TGTAACAACA CAACCAAAAT TGATTGTTCA TTGTAAGTTT 
TTAGGTGCTC CAAACCTTCC AGTGTACGTT GTAGATTGCA GCAGTTCTGG GACCAGTATT 
TTAACAGGAT CTGCGATTCA AGTGCAGAAC ATTAAAAAAG ACCAGACTCT TAAAGCAAAA 
ATTGAAATAC CTAGCTGTAA AGATGTGGCG CCTGTGGAGA AGACTATTAA GTTGCTTCCT 
AGTAGCCATG TTGCAAGATT ACAGATATTC AGTGTAGAAG GACAAAAGGC AATCCAGATC 
AAACATCAGG ATGAGGTTAA TTGGATAGCA GGCGATACTA TGCATAATCT TATTTTTCAA 
ATGTATGATG AAGGAGAAAG AGAAATTCAT ATAACATCAA CTTTAGCAGA TAAAATTAAA 
GTTAATTGGA CTCCTGAGAT TAACAAAGAA CACTTGCTAC AAGGTCTGCT TCCTGATGTA 
CAAGTACCAA CATCTGTGAA AGATATGCGT TATTGCCAGG TTTCATTCCA AGATGATCAT 
GTGTCTTTGG AAAGTGCATT TACAGTAAGA CCGCTTCCTG ATGAACCTAA ACATCTAAAA 
TGTGAATTAA AAGGAGGAAA AACAGTACAG ATGGGCCAAG AGCTTCAAGG AGAAATAGTT 
GTAATAGTTA CAGATCAGTA TGGAAATCAG ATTCAAGCAT TTTCACCAAG TTCTTTATCT 
GCCTTGGGAA TTGCTGGGAT TGGACTTGAT AGCTCTCATT TGAAAACTAC CTTTCAGGAA 
AATACACAAA GTATAAGTGT AAAAGGCATC AAATTTATTC CAGGCCCTCC TGGAAATAAG 
GATCTTTGTT TTACTTGGCG TGAATTTTCT GACTTTATTC GAGTGCAGCT AATTTCTGGA 
CCACCAGCAA AAGTTCTCCT TATAGACTGG CCAGAATTAA AGGAGTCCAT TCCAGTGATA 
AATGGAAGAG AATTACAGAA TCCTCTCATT GTTCAGCTTT GTGATCAGTG GGATAATCCA 
GCACCTGTAT CACAAGTTAA AATAAGTCTC ATAAAAGCTA ACAATTTAAA GCTCACGCCT 
TCAAATCAAC AGCATAAAAC AGATGAAAGG GGCAGGGCTA ATTTGGGAGT GTTCAGTGTT 
TATGCCCCTA GAGGAGAACA TATGATGCAT GTTAGAGCCA TCTATAACAA GAATATCATA 
GAAGGACCTA TAATTAAGTT AATGATTCTT CCAGACCCTG AAAAACCTAT TCGTCTCAAT 
GTTAAATATG ACAAAGATGC TTCCTTTCTA GCAGGGGGTA TTTTCACTGA TTTTATGATT 
ACTGTTATTT CTGAAGATGA CAGTATCATT AAAAATATTA ATCCAGCACG TATCTCCATG 
AAAATGTGGC AGCTGTCTAA CAGTGGGAAC CGACCACCAG CAAATGCAGA AACATTTAGT 
TGTAATAAAA TAAAAGATAA TGACAAGGAA GATGGCTGTT TCTACTTCAG GGATAAAGCA 
ATTCCTAACA AAGTGGGAAC ATACTGTATC CAGTTTGGTT TTATGATGGA TAAAGCAAAT 
ATTCTTAACA GTGAACAGAT TATAGTTGAT GTTCTCCCTA ATCAGCCTGT GAAGTTAGTG 
CCTAAAATCC AACCAGCTAC ACCAGCTGTT TCAAATGTTC GCTCAGTTGC CAGCAGGACC 
TTGGTCAGAG ATTTACATCT TACTATTACG GATGACTACA ACAATCATAC CGGAATTGAT 
TTGGTTGGCA CTGTAGTAGC TACCATTAAA GGATCTAATG AGGAAGATAC AGATACACCA 
CTCTTTATCG GGAAAGTGAG AACACTTGAA TTTCCCTTTG TGAAAGGTTC AGCTGAAATC 
ACGAATCTAG TGCTGGCAGA AAATAGTCCT GGAAGGGATA GTACTGAATA TTTTATTGTA 
TTTGAGCCTC AGCTACCAGC TCTGTCAAGA ACATTAGAAT CATATATCCT ACCATTTATG 
TTTTACAATG ATGTTAAGAA GCAGCAACAA ATGGCAGCAC TTACAAAAGA AAAGGACCAG 
TTATCTAAGA ATATTATAAT GTATAGAAGT TTATTTGAAG CCAGCAATCA GCTTCTTGAT 
GAAATGAAGT GCCGAGTTGA AGAAGCAAAA TTAAAAGAGG CTCAGTTGCA AAATGAACTA 
AAAACACATA ATATTGACAT CCCTACAACA CAACAGATGC CACACATTGA AGCACTTTTG 
AAAAGAAAGC TATCAGAACA GGAAGAACTG AGGAAAAAAC CTAGAAGATC GTGTACTCTT 
CCAAACTATA CTAAAGGCAG TGGAGATGTT TTGGGAAAGA TTGCACACCT GGCACAAATT 
GAAGATGATA GAGCTGCAAT GGTTATTTCT TGGCATCTGG CAAGTGACAT GGACTGTGTA 
GTCACCCTGA CCACTGATGC TGCACGTCGC ATCTATGATG AAACCCAAGG TCGTCAGCAG 
GTCTTGCCCT TGGATTCTAT TTATAAGAAG ACTCTTCCAG ATTGGAAAAG ACCTCTACCT 
CATTACCGAA ATGGAAAATT GTATTTTAAA CCCATTGGAG ATCCAGTCTT TGCCCGAGAT 
TTGTTAACAT TTCCAGATAA TGTAGAACAT TGTGAAACAG TATTTGGTAT GCTGTTAGGA 
GACACCATTA TTTTGGATAA TTTGGATGCT GCCAATCATT ATAGAAAAGA GGTTGTTAAA 
ATTACACACT GTCCTACACT GCTCACCAGA GATGGAGATC GCATTCGAAG TAACGGAAAG 
TTTGGGGGTC TTCAGAATAA AGCTCCTCCA ATGGATAAAC TTCGGGGAAT GGTATTTGGA 
GCTCCAATAC CAAAACAGTG CCTGGTCTTA GGGGAACAAA TAGATCTTCT CCAACAGTAT 
CGTACTGCTG TGAACAAGTC GAACAGTGTG ATCGAGGACC TTAACCGTCA GTTAGAGTAC 
CTTCACACTC CTGATATGAA GAAGAAAAAA CAAGAACTTG ATGAACAAGA AAAGAATCTG 
AAACTGATCG AGCAAAAACT AGGTATGACT CCCACACGGA AGTGTAATGA CTCATTGCGC 
CATCCAGCAA AGGTTGAAAT GACAGATTGT CCAATTCCTC CTAAAAGAAT GAGAAGAGAA 
GCTTCAAGAC AAAATAGTTT TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SMCHD1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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