Smchd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smchd1
Entrez Gene ID	316732
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
Synonyms	RGD1307234
General protein information	Preferred Names structural maintenance of chromosomes flexible hinge domain containing 1 Names structural maintenance of chromosomes flexible hinge domain-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q38

mRNA and Protein(s)

mRNA	Protein	Name
XM_001056555.3	XP_001056555.2	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
XM_244261.8	XP_244261.6	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMCHD1	XP_005623086.1
Bos taurus (cattle)	SMCHD1	NP_001193329.1
Gallus gallus (chicken)	SMCHD1	XP_419144.4
Danio rerio (zebrafish)	LOC570784	XP_699397.5
Pan troglodytes (chimpanzee)	SMCHD1	XP_512045.2
Macaca mulatta (Rhesus monkey)	SMCHD1	XP_001086253.2
Xenopus tropicalis (tropical clawed frog)	smchd1	XP_002938954.1
Homo sapiens (human)	SMCHD1	NP_056110.2
Mus musculus (house mouse)	Smchd1	NP_083163.3
Rattus norvegicus (Norway rat)	Smchd1	XP_244261.6

Gene Ontology

The following Smchd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smchd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa18558	XM_001056555.3 Latest version!	Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa32676	XM_244261.8 Latest version!	Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smchd1 ( XM_001056555.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001056555.2 Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa18558
Clone ID Related Accession (Same CDS sequence)	XM_001056555.3
Accession Version	XM_001056555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000077.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_001056555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGGGCGCCAA GGATCCAGGC GGCCTCTCCA AGGGGTCTGG AAGGGATGGC 
GACATCGACG GCTGTAGGAC GGTGTACTTG TTTGACCGGC GCGGGAAGGA CTCGGAGCTC 
GGGGATAGAG CACTGCAGGT CCCGGAGCGC GCGGGCTACG CGGAGTTCCG CGCGTCTGTG 
TGTCAGACAA TTGGCATTTC ATCTGAAGAA AAGTTTGTTA TTACAACTAC AAGTAGGAAA 
GAAATTACCT GCGATAATTT TGACGACACT GTTAAAGATG GAGTCACTCT GTACCTGCTG 
CAGTCAGTTG ACCAGTCGCT ACTGACAGCA ACAAAAGAAA GAATTGACTT TGTACCTCAC 
TATGACACTC TGGTTAAAAG TGGCATGTAT GAGTACTATG CAAGTGAAGG GCAGAACCCT 
TTGCCATTTG CTCTTGCAGA ATTAATCGAC AATTCATTGT CAGCTACTTC TCGAAATGTT 
GGTGTTAGGA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTC 
GCAGTAGTGG ATAATGGAAG AGGAATGACT TCTAAACAGC TTAACAACTG GGCTGTGTAC 
AGGCTGTCAA AATTCACAAG ACAAGGTGAC TTTGAAAGTG ATCACTCAGG ATATGTCCGG 
CCAGTACCAG TACCACGAAG TTTGAACAGT GATATTTCCT ATTTTGGTGT TGGAGGCAAA 
CAGGCTGTTT TCTTTGTTGG GCAATCAGCC AGAATGATAA GCAAACCTAT AGACTCCAAG 
GATGTCCATG AGCTCGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAACAAAGAG 
GCAATATATA GTGGATACAT TAGAAACAGA AAGCCAGCTG ATTCTGTTCA TATTACAAAT 
GATGATGAAA GATTCCTACA CAATCTTATT GTAGAGGAGA AAGAGAAAGA TAGCTTTACT 
GCTGTGGTTA TTACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTACTTTCAT 
CTTTGGACAC GACAGCTAAC GCATATTTAT CATTACTATA TTCATGGACC AAAAGGAAAT 
GAAATAAGTA CAGCAAAAGA TGTTGGACCT TTCAACAACA TTGATATTGA AATTTCTTTA 
TTTGAAAAAG GGAAGACACC TAAGATTGTC AACCTAAGGG AAATACAAGA TGACATGCAG 
ACATTGTATA TAAACACAGC ATCTGATAGT TTTGAGTTCA AGGCTCATGT TGAAGGAGAC 
GGTGTAGTGG AAGGTGTTAT CCGGTATCAT CCCTTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAGTTAAGA GATGAAGATG ACGATGATGA CTGCTTCATA 
CCTGAGAAAG CAGCAAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGGTTA 
ATTCCATATA CATCGGTTGA AGACTTCGAC TGGTGTGCTC CTCCTAAGAA GAGAGGGCTT 
GCCCCGATTG AGTGCTTTAA TAGAATATCT GGTGCATTAT TCACTAATGA CAAATTTCAG 
GTCAGCACAA ATAAACTGAC ATTTATGGAT CTTGAGCTGA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GAATTTTAAA TGGACAGGAG CAGAGAATGA AAATTGACAG AGAATTTGCT 
TTATGGCTGA AGGACTGTCA TGAAAAGCAC GACAAACAAA TAAAATTCAC ACTTTTTAAA 
GGAATAATTA CCCGTCCGGA TCTTCCCAGT AAAAAGCAAG GTCCCTGGGC GACATTCTCA 
GCCATAGAGT GGGACGGGAA GATATACAAA GCAGGCCAAC TGGTGAAGAC AATCAAGACC 
CTTCCTCTCT TTTATGGAAG CATTGTAAGG TTTTTCCTTC ATGGCGATCA TGATGGAGAA 
GTGTACGCTA CAGGAGGTGA AGTTCAGATT GCGATGGAAC CCCAGGCACT ATATGATGAA 
ATAAAAACTG TGCCCATTGC AAAGCTGGAT AGGACAGTAG CTGAGAAAAC AATTAGAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CTGTAACTTG GCCTGAAGGA 
GATGAATTAG TGCCAAACGA AGTCAGACCT GCTGGAACAC CTATTGGGGC ACTAAGAATT 
GAGATTCTGA ACAAAAAGGG GGAGGCAATG CAGAAGCTGC CAGGGACAAG TCACGGAGGC 
TCCAAGAAGC TGCTGGTCGA ACTCAAAGTT ATACTACACA CTTCAAGTGG AAATAAAGAA 
ATCATTTCAC ATATCAGTCA ACATGGAGGG AAATGGCCTT ACTGGTTTAA AAAAATGGAG 
AATATCCAGA AACTGGGAAA TTACACATTA AAATTACAAG TGGTATTGAA TGAAAGTAAT 
GCAGATACCT ATGCAGGAAG ATCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGCAAGCCAG AAAAGTTTTC GTTTGGTCTT CTGGACTCTC CTTTCCGTGT TGGAGTTCCA 
TTTAACATTC CTCTGGAGTT CCAGGATGAA TTTGGTCACA CTACCCAACT ACTATCTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTCTG TCTTTACATT ATGAAGAAAT AACCAAAGGC 
CCAAATTGTG TAATTCAAGG TGTCATAGCT AAGGGTCCAG TAAACTCTTG TCAAGGCAAG 
AATTTTAATC TGAAGGTTAT TCTTCCGGGT TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTGTTAC CTGGTCCCCC TCATCGACTA AAAGTGAAAC CTGACTCAGA AATTCTAGTT 
ATTGAGAATG GAACAGCTTT CCCTTTTCAG GTGGAAGTTG TGGATGAGTC AGACAACATA 
ACAGCACAAC CAAAGCTGAT TGTCCACTGT AAGTTCCTCG GTGCTCCAAA CCTACCAGTC 
TACACTGTGG ATTGCAGCAG CTCTGGAACG AGCATCCTGA CAGGGTCACC AATCCAAGTG 
CAGAACATTA AGAAAGACCA GACACTGACC GCCAGGATTG AGATACCTAG CTGTAAAGAT 
GTGTCACCTG TTGAAAAGAC TATCAAGTTA CTTCCTAGTA GCCATGTTGC TTGCCTACAA 
ATCTTTAGTG TAGAGGGACA AAAAGCAATC CAGATTAAAC ATCAGGATGA GGTTAATTGG 
ATAGCAGGTG ATGTAATGCG CAATCTGATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATTA CACCATCCTT GGCAGAAAAA ATTAAGGTTA ATTGGACTCC TGAGGTTAAC 
AAAGAACATT TGGTACAAGG TCTGCTTCCT GATGTACAAG TACCAACATC TGTGAAAGAT 
GTACGCTACT GCCAGGTTTC TTTCCAAGAT GACCATGTGT CTCTGGAAAG TGCCTTTACA 
GTAAGACCAC TTCCTGATGA TCCCAAACAT TTAAAATGTG TGTTAAAAGG AGGAAAAACA 
GTGCAGATGG GCCAAGAGCT TCAAGGAGAA ATAGTTGTAA TAATTGTAGA TCAGTATGGA 
AATCAGATTT CAGCATTCTC ATCTGATTCC TTATCTACTT TGTCAATCTC TGGAGATGGG 
CTTGACAGCA AAAACCTGAA AACCACTTTG GAGACCAGCA CACAGAGTAT CAGCGTACAG 
GGCATCAGAT TTACCCCAGG TCCTCCTGGA CCCAAGGATC TTTGTTTTAC CTGGCGAGAG 
TTTTCTGACT TCATTCGAGT GCATCTTATT TCTGGACCTC CAACCAAGCT GCTGCTTATT 
GACTGGCCAG AGCTGAAAGA GTCCATTCCG GTGATTAATG GAAGACAATT ACAGAACCCT 
CTTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCTGCTT TAGTTCCAAA TGTTAAAATA 
TGTCTCATAA AAGCAAGCAG TTTAAAGCTA GTACCTTCAA ACCAGCAGCA TAAAACAGAT 
GATAAGGGCA GAGCTAATTT GGGGATGTTC ACTGTTTTTG CTCCAAGGGG AGAACACACT 
GTACAGGTTA AAGGTGTCTA TAATAAGAGT ACTATAGAAG GACCTACAAT TAAGTTAATG 
ATTCTTCCAG ACCCTGAAAA ACCCATTCGT CTGAATGTTA AATATGACAA AGATGCCTCT 
TTTATAGCAG GGGACATATT TACTGATTTT ATGGTTAGTG TGATTTCTGA AGGTGGTAGT 
GTCATCAAGA ATATTAATCC AACACGTATT TCCATGAAAA TGTGGAAGCT GTCCAGTGGC 
ATGAGCCGGC CACCAGCAAA CGCAGAAATC TTTAGTTGTA ATAAAATAAA AGATAACGAC 
AAGGAAGATG GCTGCTTCTA TTTCAGGGAG AAAACCATTC CTTGCAAGGT GGGGGCGTAC 
TGTATTCAGT TTGATTTCAT GATCGATAAA ACTAATATGC TCAGCAGTGA ACAGGTTATA 
GTTGATGTTC TGCCTAATCA GCCTATGAAG TTGGTTCCTG ATACCCAGCC AGCTACCCCA 
GCCGTTTCTA ATGTTCGCTC CATTGCCAGC AGGACCTTGG TCAAAGACCT ACGCCTCAGT 
ATTACAGATA ATTATGGTAA TCATACTGGA ATGGATCTGG TTGGCACTGT AGTAGCAACC 
ATTAAAGGCT TTAATGAGGA GGACACTGAT ACCCCACTCT TTATCGGGAA GGTTAGGACG 
CTTGAGTTTC CCTTTGTGAA TGGTTCTGCT GAAATCACGA CTCTAGTGCT GGCAGAAAAT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTATCTTTG AGCCTCGGCT GTCAACTTTA 
TCATCAACAT TAGAATCCTA TAGCCTGCCA TTTATGTTTT ACAATGATGT TAAAAAGCAG 
CAACAAATGG CAGCGCTTAC GAAGGAAAAG GATGAGCTAT CTAAGTCCAT CACTATGTAC 
AGAAGTCTCT TTGATGCCAA CAAGCAGCTT CTGGATGAAA TGAAATGCCA AGCTGAAGAA 
GCAAAATTTA AAGAGACCCA GCTACGAAAT GAACTAAAAG CACACAATAT TGATATTCCT 
GCAACACAAC AGATACCTCA CATTGAAGCA CTTTTGGAAA AAAAGATAGC AGAACAGAAT 
GAGCTTAAAA AGAGGCCCAG AAGATTGTGT ACTCTTCCAA ACTACACTAA ACGCAGTGGA 
GATATTTTGG GAAAGATTGC ACATCTAGCA CAGATTGAAG ATGACCGAGC CGCCATGGTT 
ATTTCTTGGC ATCTAGCAAG TGACATGGAC TGTGTGGTTA CCCTGACCAC CGATGCTGCT 
CGCTGCATCT ACGATGAGAC TCAAGGTCGC CAGCAAGTGT TGCCCCTCGA TTCTATCTAC 
AGGAAAACTC TTCCAGATTG GAAAAGACCT CTACCTCATT TCCGAAATGG TAAATTACAT 
TTTAAGCCCT TTGGTGATCC AGTCTTTGCC CGAGATTTGT TAACATTCCC AGATAATATT 
GAACATTGTG AAACAGTTTT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAACCTA 
GATGCAGCCA ATCATTATAG AAAAGAGGTT GTTAAAATCA CGCACTGTCC CACACTCCTG 
ACTAGAGATG GAGATAGGAT TAGAAGTAAT GGGAAGTTTG GGGGCCTTCA GAATAAAGCC 
CCACCAATGG ACAAACTCCG GGGAATGGTC TTTGGAGCTC CAGTACCAAA GCAGTGTGTG 
GACTTGGGGA AGCAGATAGA TCTTATTCAG CAGTATCGAA ATGCTCTGTG CAAGCTGAAC 
TCCGTGAGGG CGGACTTGGA CCATCAGCTG CAGTACCTCC ACACACCCGA CATGAAGAGG 
AAGAAGCAGG AGCTCGATGA GCAGGAAAAA AGCCTGAAGC GAATCGAGCA GAAGCTCGGA 
ATGACTCCTA TGCGCAGGTG TAGTGACTCA CTTTGTCTAT CACCAAAGAT TGAGGTGACT 
GAGTGTCCAA TCCCCACTAA AAGAATGAGA AGAGAATCCA TCAGACAGAA TAGAAGACCA 
AAAGGTGATG TCCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001056555.2
CDS	311..6331
Translation	MAAEGAKDPGGLSKGSGRDGDIDGCRTVYLFDRRGKDSELGDRALQVPERAGYAEFRASVCQTIGISSEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVDQSLLTATKERIDFVPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNVGVRRIQIKLLFDETQGKPAVAVVDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPIDSKDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPADSVHITNDDERFLHNLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLTHIYHYYIHGPKGNEISTAKDVGPFNNIDIEISLFEKGKTPKIVNLREIQDDMQTLYINTASDSFEFKAHVEGDGVVEGVIRYHPFLYDRETYPDDPCFPSKLRDEDDDDDCFIPEKAARGKRPIFECFWNGRLIPYTSVEDFDWCAPPKKRGLAPIECFNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKHDKQIKFTLFKGIITRPDLPSKKQGPWATFSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLHGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKTIRKYVEDEMARLPDRLSVTWPEGDELVPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHTSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRSLPSKAIKFSVKEGKPEKFSFGLLDSPFRVGVPFNIPLEFQDEFGHTTQLLSDIQPVLEASGLSLHYEEITKGPNCVIQGVIAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVVDESDNITAQPKLIVHCKFLGAPNLPVYTVDCSSSGTSILTGSPIQVQNIKKDQTLTARIEIPSCKDVSPVEKTIKLLPSSHVACLQIFSVEGQKAIQIKHQDEVNWIAGDVMRNLIFQMYDEGEREINITPSLAEKIKVNWTPEVNKEHLVQGLLPDVQVPTSVKDVRYCQVSFQDDHVSLESAFTVRPLPDDPKHLKCVLKGGKTVQMGQELQGEIVVIIVDQYGNQISAFSSDSLSTLSISGDGLDSKNLKTTLETSTQSISVQGIRFTPGPPGPKDLCFTWREFSDFIRVHLISGPPTKLLLIDWPELKESIPVINGRQLQNPLIVQLCDQWDNPALVPNVKICLIKASSLKLVPSNQQHKTDDKGRANLGMFTVFAPRGEHTVQVKGVYNKSTIEGPTIKLMILPDPEKPIRLNVKYDKDASFIAGDIFTDFMVSVISEGGSVIKNINPTRISMKMWKLSSGMSRPPANAEIFSCNKIKDNDKEDGCFYFREKTIPCKVGAYCIQFDFMIDKTNMLSSEQVIVDVLPNQPMKLVPDTQPATPAVSNVRSIASRTLVKDLRLSITDNYGNHTGMDLVGTVVATIKGFNEEDTDTPLFIGKVRTLEFPFVNGSAEITTLVLAENSPGRDSTEYFIIFEPRLSTLSSTLESYSLPFMFYNDVKKQQQMAALTKEKDELSKSITMYRSLFDANKQLLDEMKCQAEEAKFKETQLRNELKAHNIDIPATQQIPHIEALLEKKIAEQNELKKRPRRLCTLPNYTKRSGDILGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARCIYDETQGRQQVLPLDSIYRKTLPDWKRPLPHFRNGKLHFKPFGDPVFARDLLTFPDNIEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCVDLGKQIDLIQQYRNALCKLNSVRADLDHQLQYLHTPDMKRKKQELDEQEKSLKRIEQKLGMTPMRRCSDSLCLSPKIEVTECPIPTKRMRRESIRQNRRPKGDVPN

Target ORF information:

RefSeq Version	XM_001056555.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001056555.3

ORF Insert Sequence:

ATGGCAGCGG AGGGCGCCAA GGATCCAGGC GGCCTCTCCA AGGGGTCTGG AAGGGATGGC 
GACATCGACG GCTGTAGGAC GGTGTACTTG TTTGACCGGC GCGGGAAGGA CTCGGAGCTC 
GGGGATAGAG CACTGCAGGT CCCGGAGCGC GCGGGCTACG CGGAGTTCCG CGCGTCTGTG 
TGTCAGACAA TTGGCATTTC ATCTGAAGAA AAGTTTGTTA TTACAACTAC AAGTAGGAAA 
GAAATTACCT GCGATAATTT TGACGACACT GTTAAAGATG GAGTCACTCT GTACCTGCTG 
CAGTCAGTTG ACCAGTCGCT ACTGACAGCA ACAAAAGAAA GAATTGACTT TGTACCTCAC 
TATGACACTC TGGTTAAAAG TGGCATGTAT GAGTACTATG CAAGTGAAGG GCAGAACCCT 
TTGCCATTTG CTCTTGCAGA ATTAATCGAC AATTCATTGT CAGCTACTTC TCGAAATGTT 
GGTGTTAGGA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTC 
GCAGTAGTGG ATAATGGAAG AGGAATGACT TCTAAACAGC TTAACAACTG GGCTGTGTAC 
AGGCTGTCAA AATTCACAAG ACAAGGTGAC TTTGAAAGTG ATCACTCAGG ATATGTCCGG 
CCAGTACCAG TACCACGAAG TTTGAACAGT GATATTTCCT ATTTTGGTGT TGGAGGCAAA 
CAGGCTGTTT TCTTTGTTGG GCAATCAGCC AGAATGATAA GCAAACCTAT AGACTCCAAG 
GATGTCCATG AGCTCGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAACAAAGAG 
GCAATATATA GTGGATACAT TAGAAACAGA AAGCCAGCTG ATTCTGTTCA TATTACAAAT 
GATGATGAAA GATTCCTACA CAATCTTATT GTAGAGGAGA AAGAGAAAGA TAGCTTTACT 
GCTGTGGTTA TTACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTACTTTCAT 
CTTTGGACAC GACAGCTAAC GCATATTTAT CATTACTATA TTCATGGACC AAAAGGAAAT 
GAAATAAGTA CAGCAAAAGA TGTTGGACCT TTCAACAACA TTGATATTGA AATTTCTTTA 
TTTGAAAAAG GGAAGACACC TAAGATTGTC AACCTAAGGG AAATACAAGA TGACATGCAG 
ACATTGTATA TAAACACAGC ATCTGATAGT TTTGAGTTCA AGGCTCATGT TGAAGGAGAC 
GGTGTAGTGG AAGGTGTTAT CCGGTATCAT CCCTTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAGTTAAGA GATGAAGATG ACGATGATGA CTGCTTCATA 
CCTGAGAAAG CAGCAAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGGTTA 
ATTCCATATA CATCGGTTGA AGACTTCGAC TGGTGTGCTC CTCCTAAGAA GAGAGGGCTT 
GCCCCGATTG AGTGCTTTAA TAGAATATCT GGTGCATTAT TCACTAATGA CAAATTTCAG 
GTCAGCACAA ATAAACTGAC ATTTATGGAT CTTGAGCTGA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GAATTTTAAA TGGACAGGAG CAGAGAATGA AAATTGACAG AGAATTTGCT 
TTATGGCTGA AGGACTGTCA TGAAAAGCAC GACAAACAAA TAAAATTCAC ACTTTTTAAA 
GGAATAATTA CCCGTCCGGA TCTTCCCAGT AAAAAGCAAG GTCCCTGGGC GACATTCTCA 
GCCATAGAGT GGGACGGGAA GATATACAAA GCAGGCCAAC TGGTGAAGAC AATCAAGACC 
CTTCCTCTCT TTTATGGAAG CATTGTAAGG TTTTTCCTTC ATGGCGATCA TGATGGAGAA 
GTGTACGCTA CAGGAGGTGA AGTTCAGATT GCGATGGAAC CCCAGGCACT ATATGATGAA 
ATAAAAACTG TGCCCATTGC AAAGCTGGAT AGGACAGTAG CTGAGAAAAC AATTAGAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CTGTAACTTG GCCTGAAGGA 
GATGAATTAG TGCCAAACGA AGTCAGACCT GCTGGAACAC CTATTGGGGC ACTAAGAATT 
GAGATTCTGA ACAAAAAGGG GGAGGCAATG CAGAAGCTGC CAGGGACAAG TCACGGAGGC 
TCCAAGAAGC TGCTGGTCGA ACTCAAAGTT ATACTACACA CTTCAAGTGG AAATAAAGAA 
ATCATTTCAC ATATCAGTCA ACATGGAGGG AAATGGCCTT ACTGGTTTAA AAAAATGGAG 
AATATCCAGA AACTGGGAAA TTACACATTA AAATTACAAG TGGTATTGAA TGAAAGTAAT 
GCAGATACCT ATGCAGGAAG ATCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGCAAGCCAG AAAAGTTTTC GTTTGGTCTT CTGGACTCTC CTTTCCGTGT TGGAGTTCCA 
TTTAACATTC CTCTGGAGTT CCAGGATGAA TTTGGTCACA CTACCCAACT ACTATCTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTCTG TCTTTACATT ATGAAGAAAT AACCAAAGGC 
CCAAATTGTG TAATTCAAGG TGTCATAGCT AAGGGTCCAG TAAACTCTTG TCAAGGCAAG 
AATTTTAATC TGAAGGTTAT TCTTCCGGGT TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTGTTAC CTGGTCCCCC TCATCGACTA AAAGTGAAAC CTGACTCAGA AATTCTAGTT 
ATTGAGAATG GAACAGCTTT CCCTTTTCAG GTGGAAGTTG TGGATGAGTC AGACAACATA 
ACAGCACAAC CAAAGCTGAT TGTCCACTGT AAGTTCCTCG GTGCTCCAAA CCTACCAGTC 
TACACTGTGG ATTGCAGCAG CTCTGGAACG AGCATCCTGA CAGGGTCACC AATCCAAGTG 
CAGAACATTA AGAAAGACCA GACACTGACC GCCAGGATTG AGATACCTAG CTGTAAAGAT 
GTGTCACCTG TTGAAAAGAC TATCAAGTTA CTTCCTAGTA GCCATGTTGC TTGCCTACAA 
ATCTTTAGTG TAGAGGGACA AAAAGCAATC CAGATTAAAC ATCAGGATGA GGTTAATTGG 
ATAGCAGGTG ATGTAATGCG CAATCTGATT TTTCAAATGT ATGATGAAGG AGAAAGAGAA 
ATCAATATTA CACCATCCTT GGCAGAAAAA ATTAAGGTTA ATTGGACTCC TGAGGTTAAC 
AAAGAACATT TGGTACAAGG TCTGCTTCCT GATGTACAAG TACCAACATC TGTGAAAGAT 
GTACGCTACT GCCAGGTTTC TTTCCAAGAT GACCATGTGT CTCTGGAAAG TGCCTTTACA 
GTAAGACCAC TTCCTGATGA TCCCAAACAT TTAAAATGTG TGTTAAAAGG AGGAAAAACA 
GTGCAGATGG GCCAAGAGCT TCAAGGAGAA ATAGTTGTAA TAATTGTAGA TCAGTATGGA 
AATCAGATTT CAGCATTCTC ATCTGATTCC TTATCTACTT TGTCAATCTC TGGAGATGGG 
CTTGACAGCA AAAACCTGAA AACCACTTTG GAGACCAGCA CACAGAGTAT CAGCGTACAG 
GGCATCAGAT TTACCCCAGG TCCTCCTGGA CCCAAGGATC TTTGTTTTAC CTGGCGAGAG 
TTTTCTGACT TCATTCGAGT GCATCTTATT TCTGGACCTC CAACCAAGCT GCTGCTTATT 
GACTGGCCAG AGCTGAAAGA GTCCATTCCG GTGATTAATG GAAGACAATT ACAGAACCCT 
CTTATTGTTC AACTTTGTGA TCAGTGGGAT AATCCTGCTT TAGTTCCAAA TGTTAAAATA 
TGTCTCATAA AAGCAAGCAG TTTAAAGCTA GTACCTTCAA ACCAGCAGCA TAAAACAGAT 
GATAAGGGCA GAGCTAATTT GGGGATGTTC ACTGTTTTTG CTCCAAGGGG AGAACACACT 
GTACAGGTTA AAGGTGTCTA TAATAAGAGT ACTATAGAAG GACCTACAAT TAAGTTAATG 
ATTCTTCCAG ACCCTGAAAA ACCCATTCGT CTGAATGTTA AATATGACAA AGATGCCTCT 
TTTATAGCAG GGGACATATT TACTGATTTT ATGGTTAGTG TGATTTCTGA AGGTGGTAGT 
GTCATCAAGA ATATTAATCC AACACGTATT TCCATGAAAA TGTGGAAGCT GTCCAGTGGC 
ATGAGCCGGC CACCAGCAAA CGCAGAAATC TTTAGTTGTA ATAAAATAAA AGATAACGAC 
AAGGAAGATG GCTGCTTCTA TTTCAGGGAG AAAACCATTC CTTGCAAGGT GGGGGCGTAC 
TGTATTCAGT TTGATTTCAT GATCGATAAA ACTAATATGC TCAGCAGTGA ACAGGTTATA 
GTTGATGTTC TGCCTAATCA GCCTATGAAG TTGGTTCCTG ATACCCAGCC AGCTACCCCA 
GCCGTTTCTA ATGTTCGCTC CATTGCCAGC AGGACCTTGG TCAAAGACCT ACGCCTCAGT 
ATTACAGATA ATTATGGTAA TCATACTGGA ATGGATCTGG TTGGCACTGT AGTAGCAACC 
ATTAAAGGCT TTAATGAGGA GGACACTGAT ACCCCACTCT TTATCGGGAA GGTTAGGACG 
CTTGAGTTTC CCTTTGTGAA TGGTTCTGCT GAAATCACGA CTCTAGTGCT GGCAGAAAAT 
AGTCCTGGAA GGGATAGTAC TGAATATTTT ATTATCTTTG AGCCTCGGCT GTCAACTTTA 
TCATCAACAT TAGAATCCTA TAGCCTGCCA TTTATGTTTT ACAATGATGT TAAAAAGCAG 
CAACAAATGG CAGCGCTTAC GAAGGAAAAG GATGAGCTAT CTAAGTCCAT CACTATGTAC 
AGAAGTCTCT TTGATGCCAA CAAGCAGCTT CTGGATGAAA TGAAATGCCA AGCTGAAGAA 
GCAAAATTTA AAGAGACCCA GCTACGAAAT GAACTAAAAG CACACAATAT TGATATTCCT 
GCAACACAAC AGATACCTCA CATTGAAGCA CTTTTGGAAA AAAAGATAGC AGAACAGAAT 
GAGCTTAAAA AGAGGCCCAG AAGATTGTGT ACTCTTCCAA ACTACACTAA ACGCAGTGGA 
GATATTTTGG GAAAGATTGC ACATCTAGCA CAGATTGAAG ATGACCGAGC CGCCATGGTT 
ATTTCTTGGC ATCTAGCAAG TGACATGGAC TGTGTGGTTA CCCTGACCAC CGATGCTGCT 
CGCTGCATCT ACGATGAGAC TCAAGGTCGC CAGCAAGTGT TGCCCCTCGA TTCTATCTAC 
AGGAAAACTC TTCCAGATTG GAAAAGACCT CTACCTCATT TCCGAAATGG TAAATTACAT 
TTTAAGCCCT TTGGTGATCC AGTCTTTGCC CGAGATTTGT TAACATTCCC AGATAATATT 
GAACATTGTG AAACAGTTTT TGGTATGCTG TTAGGAGACA CCATTATTTT GGATAACCTA 
GATGCAGCCA ATCATTATAG AAAAGAGGTT GTTAAAATCA CGCACTGTCC CACACTCCTG 
ACTAGAGATG GAGATAGGAT TAGAAGTAAT GGGAAGTTTG GGGGCCTTCA GAATAAAGCC 
CCACCAATGG ACAAACTCCG GGGAATGGTC TTTGGAGCTC CAGTACCAAA GCAGTGTGTG 
GACTTGGGGA AGCAGATAGA TCTTATTCAG CAGTATCGAA ATGCTCTGTG CAAGCTGAAC 
TCCGTGAGGG CGGACTTGGA CCATCAGCTG CAGTACCTCC ACACACCCGA CATGAAGAGG 
AAGAAGCAGG AGCTCGATGA GCAGGAAAAA AGCCTGAAGC GAATCGAGCA GAAGCTCGGA 
ATGACTCCTA TGCGCAGGTG TAGTGACTCA CTTTGTCTAT CACCAAAGAT TGAGGTGACT 
GAGTGTCCAA TCCCCACTAA AAGAATGAGA AGAGAATCCA TCAGACAGAA TAGAAGACCA 
AAAGGTGATG TCCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smchd1 ( XM_244261.8 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_244261.6 Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X1, mRNA.

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CloneID	ORa32676
Clone ID Related Accession (Same CDS sequence)	XM_244261.8
Accession Version	XM_244261.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_244261.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AGGGCGCCAA GGATCCAGGC GGCCTCTCCA AGGGGTCTGG AAGGGATGGC 
GACATCGACG GCTGTAGGAC GGTGTACTTG TTTGACCGGC GCGGGAAGGA CTCGGAGCTC 
GGGGATAGAG CACTGCAGGT CCCGGAGCGC GCGGGCTACG CGGAGTTCCG CGCGTCTGTG 
TGTCAGACAA TTGGCATTTC ATCTGAAGAA AAGTTTGTTA TTACAACTAC AAGTAGGAAA 
GAAATTACCT GCGATAATTT TGACGACACT GTTAAAGATG GAGTCACTCT GTACCTGCTG 
CAGTCAGTTG ACCAGTCGCT ACTGACAGCA ACAAAAGAAA GAATTGACTT TGTACCTCAC 
TATGACACTC TGGTTAAAAG TGGCATGTAT GAGTACTATG CAAGTGAAGG GCAGAACCCT 
TTGCCATTTG CTCTTGCAGA ATTAATCGAC AATTCATTGT CAGCTACTTC TCGAAATGTT 
GGTGTTAGGA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTC 
GCAGTAGTGG ATAATGGAAG AGGAATGACT TCTAAACAGC TTAACAACTG GGCTGTGTAC 
AGGCTGTCAA AATTCACAAG ACAAGGTGAC TTTGAAAGTG ATCACTCAGG ATATGTCCGG 
CCAGTACCAG TACCACGAAG TTTGAACAGT GATATTTCCT ATTTTGGTGT TGGAGGCAAA 
CAGGCTGTTT TCTTTGTTGG GCAATCAGCC AGAATGATAA GCAAACCTAT AGACTCCAAG 
GATGTCCATG AGCTCGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAACAAAGAG 
GCAATATATA GTGGATACAT TAGAAACAGA AAGCCAGCTG ATTCTGTTCA TATTACAAAT 
GATGATGAAA GATTCCTACA CAATCTTATT GTAGAGGAGA AAGAGAAAGA TAGCTTTACT 
GCTGTGGTTA TTACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTACTTTCAT 
CTTTGGACAC GACAGCTAAC GCATATTTAT CATTACTATA TTCATGGACC AAAAGGAAAT 
GAAATAAGTA CAGCAAAAGA TGTTGGACCT TTCAACAACA TTGATATTGA AATTTCTTTA 
TTTGAAAAAG GGAAGACACC TAAGATTGTC AACCTAAGGG AAATACAAGA TGACATGCAG 
ACATTGTATA TAAACACAGC ATCTGATAGT TTTGAGTTCA AGGCTCATGT TGAAGGAGAC 
GGTGTAGTGG AAGGTGTTAT CCGGTATCAT CCCTTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAGTTAAGA GATGAAGATG ACGATGATGA CTGCTTCATA 
CCTGAGAAAG CAGCAAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGGTTA 
ATTCCATATA CATCGGTTGA AGACTTCGAC TGGTGTGCTC CTCCTAAGAA GAGAGGGCTT 
GCCCCGATTG AGTGCTTTAA TAGAATATCT GGTGCATTAT TCACTAATGA CAAATTTCAG 
GTCAGCACAA ATAAACTGAC ATTTATGGAT CTTGAGCTGA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GAATTTTAAA TGGACAGGAG CAGAGAATGA AAATTGACAG AGAATTTGCT 
TTATGGCTGA AGGACTGTCA TGAAAAGCAC GACAAACAAA TAAAATTCAC ACTTTTTAAA 
GGAATAATTA CCCGTCCGGA TCTTCCCAGT AAAAAGCAAG GTCCCTGGGC GACATTCTCA 
GCCATAGAGT GGGACGGGAA GATATACAAA GCAGGCCAAC TGGTGAAGAC AATCAAGACC 
CTTCCTCTCT TTTATGGAAG CATTGTAAGG TTTTTCCTTC ATGGCGATCA TGATGGAGAA 
GTGTACGCTA CAGGAGGTGA AGTTCAGATT GCGATGGAAC CCCAGGCACT ATATGATGAA 
ATAAAAACTG TGCCCATTGC AAAGCTGGAT AGGACAGTAG CTGAGAAAAC AATTAGAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CTGTAACTTG GCCTGAAGGA 
GATGAATTAG TGCCAAACGA AGTCAGACCT GCTGGAACAC CTATTGGGGC ACTAAGAATT 
GAGATTCTGA ACAAAAAGGG GGAGGCAATG CAGAAGCTGC CAGGGACAAG TCACGGAGGC 
TCCAAGAAGC TGCTGGTCGA ACTCAAAGTT ATACTACACA CTTCAAGTGG AAATAAAGAA 
ATCATTTCAC ATATCAGTCA ACATGGAGGG AAATGGCCTT ACTGGTTTAA AAAAATGGAG 
AATATCCAGA AACTGGGAAA TTACACATTA AAATTACAAG TGGTATTGAA TGAAAGTAAT 
GCAGATACCT ATGCAGGAAG ATCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGCAAGCCAG AAAAGTTTTC GTTTGGTCTT CTGGACTCTC CTTTCCGTGT TGGAGTTCCA 
TTTAACATTC CTCTGGAGTT CCAGGATGAA TTTGGTCACA CTACCCAACT ACTATCTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTCTG TCTTTACATT ATGAAGAAAT AACCAAAGGC 
CCAAATTGTG TAATTCAAGG TGTCATAGCT AAGGGTCCAG TAAACTCTTG TCAAGGCAAG 
AATTTTAATC TGAAGGTTAT TCTTCCGGGT TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTGTTAC CTGGTCCCCC TCATCGACTA AAAGTGAAAC CTGACTCAGA AATTCTAGTT 
ATTGAGAATG GAACAGCTTT CCCTTTTCAG GTGGAAGTTG TGGATGAGTC AGACAACATA 
ACAGCACAAC CAAAGCTGAT TGTCCAAGGT TGTTGTGTGA TTGTCCCCTG TAAGTTCCTC 
GGTGCTCCAA ACCTACCAGT CTACACTGTG GATTGCAGCA GCTCTGGAAC GAGCATCCTG 
ACAGGGTCAC CAATCCAAGT GCAGAACATT AAGAAAGACC AGACACTGAC CGCCAGGATT 
GAGATACCTA GCTGTAAAGA TGTGTCACCT GTTGAAAAGA CTATCAAGTT ACTTCCTAGT 
AGCCATGTTG CTTGCCTACA AATCTTTAGT GTAGAGGGAC AAAAAGCAAT CCAGATTAAA 
CATCAGGATG AGGTTAATTG GATAGCAGGT GATGTAATGC GCAATCTGAT TTTTCAAATG 
TATGATGAAG GAGAAAGAGA AATCAATATT ACACCATCCT TGGCAGAAAA AATTAAGGTT 
AATTGGACTC CTGAGGTTAA CAAAGAACAT TTGGTACAAG GTCTGCTTCC TGATGTACAA 
GTACCAACAT CTGTGAAAGA TGTACGCTAC TGCCAGGTTT CTTTCCAAGA TGACCATGTG 
TCTCTGGAAA GTGCCTTTAC AGTAAGACCA CTTCCTGATG ATCCCAAACA TTTAAAATGT 
GTGTTAAAAG GAGGAAAAAC AGTGCAGATG GGCCAAGAGC TTCAAGGAGA AATAGTTGTA 
ATAATTGTAG ATCAGTATGG AAATCAGATT TCAGCATTCT CATCTGATTC CTTATCTACT 
TTGTCAATCT CTGGAGATGG GCTTGACAGC AAAAACCTGA AAACCACTTT GGAGACCAGC 
ACACAGAGTA TCAGCGTACA GGGCATCAGA TTTACCCCAG GTCCTCCTGG ACCCAAGGAT 
CTTTGTTTTA CCTGGCGAGA GTTTTCTGAC TTCATTCGAG TGCATCTTAT TTCTGGACCT 
CCAACCAAGC TGCTGCTTAT TGACTGGCCA GAGCTGAAAG AGTCCATTCC GGTGATTAAT 
GGAAGACAAT TACAGAACCC TCTTATTGTT CAACTTTGTG ATCAGTGGGA TAATCCTGCT 
TTAGTTCCAA ATGTTAAAAT ATGTCTCATA AAAGCAAGCA GTTTAAAGCT AGTACCTTCA 
AACCAGCAGC ATAAACCAGA TGATAAGGGC AGAGCTAATT TGGGGATGTT CACTGTTTTT 
GCTCCAAGGG GAGAACACAC TGTACAGGTT AAAGGTGTCT ATAATAAGAG TACTATAGAA 
GGACCTACAA TTAAGTTAAT GATTCTTCCA GACCCTGAAA AACCCATTCG TCTGAATGTT 
AAATATGACA AAGATGCCTC TTTTATAGCA GGGGACATAT TTACTGATTT TATGGTTAGT 
GTGATTTCTG AAGGTGGTAG TGTCATCAAG AATATTAATC CAACACGTAT TTCCATGAAA 
ATGTGGAAGC TGTCCAGTGG CATGAGCCGG CCACCAGCAA ACGCAGAAAT CTTTAGTTGT 
AATAAAATAA AAGATAACGA CAAGGAAGAT GGCTGCTTCT ATTTCAGGGA GAAAACCATT 
CCTTGCAAGG TGGGGGCGTA CTGTATTCAG TTTGATTTCA TGATCGATAA AACTAATATG 
CTCAGCAGTG AACAGGTTAT AGTTGATGTT CTGCCTAATC AGCCTATGAA GTTGGTTCCT 
GATACCCAGC CAGCTACCCC AGCCGTTTCT AATGTTCGCT CCATTGCCAG CAGGACCTTG 
GTCAAAGACC TACGCCTCAG TATTACAGAT AATTATGGTA ATCATACTGG AATGGATCTG 
GTTGGCACTG TAGTAGCAAC CATTAAAGGC TTTAATGAGG AGGACACTGA TACCCCACTC 
TTTATCGGGA AGGTTAGGAC GCTTGAGTTT CCCTTTGTGA ATGGTTCTGC TGAAATCACG 
ACTCTAGTGC TGGCAGAAAA TAGTCCTGGA AGGGATAGTA CTGAATATTT TATTATCTTT 
GAGCCTCGGC TGTCAACTTT ATCATCAACA TTAGAATCCT ATAGCCTGCC ATTTATGTTT 
TACAATGATG TTAAAAAGCA GCAACAAATG GCAGCGCTTA CGAAGGAAAA GGATGAGCTA 
TCTAAGTCCA TCACTATGTA CAGAAGTCTC TTTGATGCCA ACAAGCAGCT TCTGGATGAA 
ATGAAATGCC AAGCTGAAGA AGCAAAATTT AAAGAGACCC AGCTACGAAA TGAACTAAAA 
GCACACAATA TTGATATTCC TGCAACACAA CAGATACCTC ACATTGAAGC ACTTTTGGAA 
AAAAAGATAG CAGAACAGAA TGAGCTTAAA AAGAGGCCCA GAAGATTGTG TACTCTTCCA 
AACTACACTA AACGCAGTGG AGATATTTTG GGAAAGATTG CACATCTAGC ACAGATTGAA 
GATGACCGAG CCGCCATGGT TATTTCTTGG CATCTAGCAA GTGACATGGA CTGTGTGGTT 
ACCCTGACCA CCGATGCTGC TCGCTGCATC TACGATGAGA CTCAAGGTCG CCAGCAAGTG 
TTGCCCCTCG ATTCTATCTA CAGGAAAACT CTTCCAGATT GGAAAAGACC TCTACCTCAT 
TTCCGAAATG GTAAATTACA TTTTAAGCCC TTTGGTGATC CAGTCTTTGC CCGAGATTTG 
TTAACATTCC CAGATAATAT TGAACATTGT GAAACAGTTT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAACCT AGATGCAGCC AATCATTATA GAAAAGAGGT TGTTAAAATC 
ACGCACTGTC CCACACTCCT GACTAGAGAT GGAGATAGGA TTAGAAGTAA TGGGAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCACCAATG GACAAACTCC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AGCAGTGTGT GGACTTGGGG AAGCAGATAG ATCTTATTCA GCAGTATCGA 
AATGCTCTGT GCAAGCTGAA CTCCGTGAGG GCGGACTTGG ACCATCAGCT GCAGTACCTC 
CACACACCCG ACATGAAGAG GAAGAAGCAG GAGCTCGATG AGCAGGAAAA AAGCCTGAAG 
CGAATCGAGC AGAAGCTCGG AATGACTCCT ATGCGCAGGT GTAGTGACTC ACTTTGTCTA 
TCACCAAAGA TTGAGGTGAC TGAGTGTCCA ATCCCCACTA AAAGAATGAG AAGAGAATCC 
ATCAGACAGA ATAGAAGACC AAAAGGTGAT GTCCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_244261.6
CDS	311..6352
Translation	MAAEGAKDPGGLSKGSGRDGDIDGCRTVYLFDRRGKDSELGDRALQVPERAGYAEFRASVCQTIGISSEEKFVITTTSRKEITCDNFDDTVKDGVTLYLLQSVDQSLLTATKERIDFVPHYDTLVKSGMYEYYASEGQNPLPFALAELIDNSLSATSRNVGVRRIQIKLLFDETQGKPAVAVVDNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPIDSKDVHELVLSKEDFEKKEKNKEAIYSGYIRNRKPADSVHITNDDERFLHNLIVEEKEKDSFTAVVITGVQPEHIQYLKNYFHLWTRQLTHIYHYYIHGPKGNEISTAKDVGPFNNIDIEISLFEKGKTPKIVNLREIQDDMQTLYINTASDSFEFKAHVEGDGVVEGVIRYHPFLYDRETYPDDPCFPSKLRDEDDDDDCFIPEKAARGKRPIFECFWNGRLIPYTSVEDFDWCAPPKKRGLAPIECFNRISGALFTNDKFQVSTNKLTFMDLELKLKDKNTLFTRILNGQEQRMKIDREFALWLKDCHEKHDKQIKFTLFKGIITRPDLPSKKQGPWATFSAIEWDGKIYKAGQLVKTIKTLPLFYGSIVRFFLHGDHDGEVYATGGEVQIAMEPQALYDEIKTVPIAKLDRTVAEKTIRKYVEDEMARLPDRLSVTWPEGDELVPNEVRPAGTPIGALRIEILNKKGEAMQKLPGTSHGGSKKLLVELKVILHTSSGNKEIISHISQHGGKWPYWFKKMENIQKLGNYTLKLQVVLNESNADTYAGRSLPSKAIKFSVKEGKPEKFSFGLLDSPFRVGVPFNIPLEFQDEFGHTTQLLSDIQPVLEASGLSLHYEEITKGPNCVIQGVIAKGPVNSCQGKNFNLKVILPGLKEDSQILKIRLLPGPPHRLKVKPDSEILVIENGTAFPFQVEVVDESDNITAQPKLIVQGCCVIVPCKFLGAPNLPVYTVDCSSSGTSILTGSPIQVQNIKKDQTLTARIEIPSCKDVSPVEKTIKLLPSSHVACLQIFSVEGQKAIQIKHQDEVNWIAGDVMRNLIFQMYDEGEREINITPSLAEKIKVNWTPEVNKEHLVQGLLPDVQVPTSVKDVRYCQVSFQDDHVSLESAFTVRPLPDDPKHLKCVLKGGKTVQMGQELQGEIVVIIVDQYGNQISAFSSDSLSTLSISGDGLDSKNLKTTLETSTQSISVQGIRFTPGPPGPKDLCFTWREFSDFIRVHLISGPPTKLLLIDWPELKESIPVINGRQLQNPLIVQLCDQWDNPALVPNVKICLIKASSLKLVPSNQQHKPDDKGRANLGMFTVFAPRGEHTVQVKGVYNKSTIEGPTIKLMILPDPEKPIRLNVKYDKDASFIAGDIFTDFMVSVISEGGSVIKNINPTRISMKMWKLSSGMSRPPANAEIFSCNKIKDNDKEDGCFYFREKTIPCKVGAYCIQFDFMIDKTNMLSSEQVIVDVLPNQPMKLVPDTQPATPAVSNVRSIASRTLVKDLRLSITDNYGNHTGMDLVGTVVATIKGFNEEDTDTPLFIGKVRTLEFPFVNGSAEITTLVLAENSPGRDSTEYFIIFEPRLSTLSSTLESYSLPFMFYNDVKKQQQMAALTKEKDELSKSITMYRSLFDANKQLLDEMKCQAEEAKFKETQLRNELKAHNIDIPATQQIPHIEALLEKKIAEQNELKKRPRRLCTLPNYTKRSGDILGKIAHLAQIEDDRAAMVISWHLASDMDCVVTLTTDAARCIYDETQGRQQVLPLDSIYRKTLPDWKRPLPHFRNGKLHFKPFGDPVFARDLLTFPDNIEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCVDLGKQIDLIQQYRNALCKLNSVRADLDHQLQYLHTPDMKRKKQELDEQEKSLKRIEQKLGMTPMRRCSDSLCLSPKIEVTECPIPTKRMRRESIRQNRRPKGDVPN

Target ORF information:

RefSeq Version	XM_244261.8
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus structural maintenance of chromosomes flexible hinge domain containing 1 (Smchd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_244261.8

ORF Insert Sequence:

ATGGCAGCGG AGGGCGCCAA GGATCCAGGC GGCCTCTCCA AGGGGTCTGG AAGGGATGGC 
GACATCGACG GCTGTAGGAC GGTGTACTTG TTTGACCGGC GCGGGAAGGA CTCGGAGCTC 
GGGGATAGAG CACTGCAGGT CCCGGAGCGC GCGGGCTACG CGGAGTTCCG CGCGTCTGTG 
TGTCAGACAA TTGGCATTTC ATCTGAAGAA AAGTTTGTTA TTACAACTAC AAGTAGGAAA 
GAAATTACCT GCGATAATTT TGACGACACT GTTAAAGATG GAGTCACTCT GTACCTGCTG 
CAGTCAGTTG ACCAGTCGCT ACTGACAGCA ACAAAAGAAA GAATTGACTT TGTACCTCAC 
TATGACACTC TGGTTAAAAG TGGCATGTAT GAGTACTATG CAAGTGAAGG GCAGAACCCT 
TTGCCATTTG CTCTTGCAGA ATTAATCGAC AATTCATTGT CAGCTACTTC TCGAAATGTT 
GGTGTTAGGA GAATACAGAT CAAATTGCTT TTTGATGAAA CACAAGGAAA ACCTGCTGTC 
GCAGTAGTGG ATAATGGAAG AGGAATGACT TCTAAACAGC TTAACAACTG GGCTGTGTAC 
AGGCTGTCAA AATTCACAAG ACAAGGTGAC TTTGAAAGTG ATCACTCAGG ATATGTCCGG 
CCAGTACCAG TACCACGAAG TTTGAACAGT GATATTTCCT ATTTTGGTGT TGGAGGCAAA 
CAGGCTGTTT TCTTTGTTGG GCAATCAGCC AGAATGATAA GCAAACCTAT AGACTCCAAG 
GATGTCCATG AGCTCGTGCT TTCTAAAGAA GATTTTGAGA AGAAGGAGAA AAACAAAGAG 
GCAATATATA GTGGATACAT TAGAAACAGA AAGCCAGCTG ATTCTGTTCA TATTACAAAT 
GATGATGAAA GATTCCTACA CAATCTTATT GTAGAGGAGA AAGAGAAAGA TAGCTTTACT 
GCTGTGGTTA TTACAGGGGT ACAACCAGAA CACATACAGT ACTTGAAAAA TTACTTTCAT 
CTTTGGACAC GACAGCTAAC GCATATTTAT CATTACTATA TTCATGGACC AAAAGGAAAT 
GAAATAAGTA CAGCAAAAGA TGTTGGACCT TTCAACAACA TTGATATTGA AATTTCTTTA 
TTTGAAAAAG GGAAGACACC TAAGATTGTC AACCTAAGGG AAATACAAGA TGACATGCAG 
ACATTGTATA TAAACACAGC ATCTGATAGT TTTGAGTTCA AGGCTCATGT TGAAGGAGAC 
GGTGTAGTGG AAGGTGTTAT CCGGTATCAT CCCTTCTTAT ATGATAGAGA AACTTACCCT 
GATGATCCAT GCTTTCCATC AAAGTTAAGA GATGAAGATG ACGATGATGA CTGCTTCATA 
CCTGAGAAAG CAGCAAGAGG GAAAAGGCCT ATTTTTGAAT GTTTTTGGAA TGGACGGTTA 
ATTCCATATA CATCGGTTGA AGACTTCGAC TGGTGTGCTC CTCCTAAGAA GAGAGGGCTT 
GCCCCGATTG AGTGCTTTAA TAGAATATCT GGTGCATTAT TCACTAATGA CAAATTTCAG 
GTCAGCACAA ATAAACTGAC ATTTATGGAT CTTGAGCTGA AATTGAAAGA TAAGAACACC 
CTTTTTACAA GAATTTTAAA TGGACAGGAG CAGAGAATGA AAATTGACAG AGAATTTGCT 
TTATGGCTGA AGGACTGTCA TGAAAAGCAC GACAAACAAA TAAAATTCAC ACTTTTTAAA 
GGAATAATTA CCCGTCCGGA TCTTCCCAGT AAAAAGCAAG GTCCCTGGGC GACATTCTCA 
GCCATAGAGT GGGACGGGAA GATATACAAA GCAGGCCAAC TGGTGAAGAC AATCAAGACC 
CTTCCTCTCT TTTATGGAAG CATTGTAAGG TTTTTCCTTC ATGGCGATCA TGATGGAGAA 
GTGTACGCTA CAGGAGGTGA AGTTCAGATT GCGATGGAAC CCCAGGCACT ATATGATGAA 
ATAAAAACTG TGCCCATTGC AAAGCTGGAT AGGACAGTAG CTGAGAAAAC AATTAGAAAA 
TATGTAGAAG ATGAAATGGC AAGGCTCCCT GATAGATTGT CTGTAACTTG GCCTGAAGGA 
GATGAATTAG TGCCAAACGA AGTCAGACCT GCTGGAACAC CTATTGGGGC ACTAAGAATT 
GAGATTCTGA ACAAAAAGGG GGAGGCAATG CAGAAGCTGC CAGGGACAAG TCACGGAGGC 
TCCAAGAAGC TGCTGGTCGA ACTCAAAGTT ATACTACACA CTTCAAGTGG AAATAAAGAA 
ATCATTTCAC ATATCAGTCA ACATGGAGGG AAATGGCCTT ACTGGTTTAA AAAAATGGAG 
AATATCCAGA AACTGGGAAA TTACACATTA AAATTACAAG TGGTATTGAA TGAAAGTAAT 
GCAGATACCT ATGCAGGAAG ATCACTACCA TCTAAAGCAA TTAAGTTTTC TGTTAAAGAG 
GGCAAGCCAG AAAAGTTTTC GTTTGGTCTT CTGGACTCTC CTTTCCGTGT TGGAGTTCCA 
TTTAACATTC CTCTGGAGTT CCAGGATGAA TTTGGTCACA CTACCCAACT ACTATCTGAT 
ATTCAGCCAG TTCTTGAAGC AAGTGGTCTG TCTTTACATT ATGAAGAAAT AACCAAAGGC 
CCAAATTGTG TAATTCAAGG TGTCATAGCT AAGGGTCCAG TAAACTCTTG TCAAGGCAAG 
AATTTTAATC TGAAGGTTAT TCTTCCGGGT TTAAAAGAAG ACTCACAGAT TTTGAAAATT 
AGATTGTTAC CTGGTCCCCC TCATCGACTA AAAGTGAAAC CTGACTCAGA AATTCTAGTT 
ATTGAGAATG GAACAGCTTT CCCTTTTCAG GTGGAAGTTG TGGATGAGTC AGACAACATA 
ACAGCACAAC CAAAGCTGAT TGTCCAAGGT TGTTGTGTGA TTGTCCCCTG TAAGTTCCTC 
GGTGCTCCAA ACCTACCAGT CTACACTGTG GATTGCAGCA GCTCTGGAAC GAGCATCCTG 
ACAGGGTCAC CAATCCAAGT GCAGAACATT AAGAAAGACC AGACACTGAC CGCCAGGATT 
GAGATACCTA GCTGTAAAGA TGTGTCACCT GTTGAAAAGA CTATCAAGTT ACTTCCTAGT 
AGCCATGTTG CTTGCCTACA AATCTTTAGT GTAGAGGGAC AAAAAGCAAT CCAGATTAAA 
CATCAGGATG AGGTTAATTG GATAGCAGGT GATGTAATGC GCAATCTGAT TTTTCAAATG 
TATGATGAAG GAGAAAGAGA AATCAATATT ACACCATCCT TGGCAGAAAA AATTAAGGTT 
AATTGGACTC CTGAGGTTAA CAAAGAACAT TTGGTACAAG GTCTGCTTCC TGATGTACAA 
GTACCAACAT CTGTGAAAGA TGTACGCTAC TGCCAGGTTT CTTTCCAAGA TGACCATGTG 
TCTCTGGAAA GTGCCTTTAC AGTAAGACCA CTTCCTGATG ATCCCAAACA TTTAAAATGT 
GTGTTAAAAG GAGGAAAAAC AGTGCAGATG GGCCAAGAGC TTCAAGGAGA AATAGTTGTA 
ATAATTGTAG ATCAGTATGG AAATCAGATT TCAGCATTCT CATCTGATTC CTTATCTACT 
TTGTCAATCT CTGGAGATGG GCTTGACAGC AAAAACCTGA AAACCACTTT GGAGACCAGC 
ACACAGAGTA TCAGCGTACA GGGCATCAGA TTTACCCCAG GTCCTCCTGG ACCCAAGGAT 
CTTTGTTTTA CCTGGCGAGA GTTTTCTGAC TTCATTCGAG TGCATCTTAT TTCTGGACCT 
CCAACCAAGC TGCTGCTTAT TGACTGGCCA GAGCTGAAAG AGTCCATTCC GGTGATTAAT 
GGAAGACAAT TACAGAACCC TCTTATTGTT CAACTTTGTG ATCAGTGGGA TAATCCTGCT 
TTAGTTCCAA ATGTTAAAAT ATGTCTCATA AAAGCAAGCA GTTTAAAGCT AGTACCTTCA 
AACCAGCAGC ATAAACCAGA TGATAAGGGC AGAGCTAATT TGGGGATGTT CACTGTTTTT 
GCTCCAAGGG GAGAACACAC TGTACAGGTT AAAGGTGTCT ATAATAAGAG TACTATAGAA 
GGACCTACAA TTAAGTTAAT GATTCTTCCA GACCCTGAAA AACCCATTCG TCTGAATGTT 
AAATATGACA AAGATGCCTC TTTTATAGCA GGGGACATAT TTACTGATTT TATGGTTAGT 
GTGATTTCTG AAGGTGGTAG TGTCATCAAG AATATTAATC CAACACGTAT TTCCATGAAA 
ATGTGGAAGC TGTCCAGTGG CATGAGCCGG CCACCAGCAA ACGCAGAAAT CTTTAGTTGT 
AATAAAATAA AAGATAACGA CAAGGAAGAT GGCTGCTTCT ATTTCAGGGA GAAAACCATT 
CCTTGCAAGG TGGGGGCGTA CTGTATTCAG TTTGATTTCA TGATCGATAA AACTAATATG 
CTCAGCAGTG AACAGGTTAT AGTTGATGTT CTGCCTAATC AGCCTATGAA GTTGGTTCCT 
GATACCCAGC CAGCTACCCC AGCCGTTTCT AATGTTCGCT CCATTGCCAG CAGGACCTTG 
GTCAAAGACC TACGCCTCAG TATTACAGAT AATTATGGTA ATCATACTGG AATGGATCTG 
GTTGGCACTG TAGTAGCAAC CATTAAAGGC TTTAATGAGG AGGACACTGA TACCCCACTC 
TTTATCGGGA AGGTTAGGAC GCTTGAGTTT CCCTTTGTGA ATGGTTCTGC TGAAATCACG 
ACTCTAGTGC TGGCAGAAAA TAGTCCTGGA AGGGATAGTA CTGAATATTT TATTATCTTT 
GAGCCTCGGC TGTCAACTTT ATCATCAACA TTAGAATCCT ATAGCCTGCC ATTTATGTTT 
TACAATGATG TTAAAAAGCA GCAACAAATG GCAGCGCTTA CGAAGGAAAA GGATGAGCTA 
TCTAAGTCCA TCACTATGTA CAGAAGTCTC TTTGATGCCA ACAAGCAGCT TCTGGATGAA 
ATGAAATGCC AAGCTGAAGA AGCAAAATTT AAAGAGACCC AGCTACGAAA TGAACTAAAA 
GCACACAATA TTGATATTCC TGCAACACAA CAGATACCTC ACATTGAAGC ACTTTTGGAA 
AAAAAGATAG CAGAACAGAA TGAGCTTAAA AAGAGGCCCA GAAGATTGTG TACTCTTCCA 
AACTACACTA AACGCAGTGG AGATATTTTG GGAAAGATTG CACATCTAGC ACAGATTGAA 
GATGACCGAG CCGCCATGGT TATTTCTTGG CATCTAGCAA GTGACATGGA CTGTGTGGTT 
ACCCTGACCA CCGATGCTGC TCGCTGCATC TACGATGAGA CTCAAGGTCG CCAGCAAGTG 
TTGCCCCTCG ATTCTATCTA CAGGAAAACT CTTCCAGATT GGAAAAGACC TCTACCTCAT 
TTCCGAAATG GTAAATTACA TTTTAAGCCC TTTGGTGATC CAGTCTTTGC CCGAGATTTG 
TTAACATTCC CAGATAATAT TGAACATTGT GAAACAGTTT TTGGTATGCT GTTAGGAGAC 
ACCATTATTT TGGATAACCT AGATGCAGCC AATCATTATA GAAAAGAGGT TGTTAAAATC 
ACGCACTGTC CCACACTCCT GACTAGAGAT GGAGATAGGA TTAGAAGTAA TGGGAAGTTT 
GGGGGCCTTC AGAATAAAGC CCCACCAATG GACAAACTCC GGGGAATGGT CTTTGGAGCT 
CCAGTACCAA AGCAGTGTGT GGACTTGGGG AAGCAGATAG ATCTTATTCA GCAGTATCGA 
AATGCTCTGT GCAAGCTGAA CTCCGTGAGG GCGGACTTGG ACCATCAGCT GCAGTACCTC 
CACACACCCG ACATGAAGAG GAAGAAGCAG GAGCTCGATG AGCAGGAAAA AAGCCTGAAG 
CGAATCGAGC AGAAGCTCGG AATGACTCCT ATGCGCAGGT GTAGTGACTC ACTTTGTCTA 
TCACCAAAGA TTGAGGTGAC TGAGTGTCCA ATCCCCACTA AAAGAATGAG AAGAGAATCC 
ATCAGACAGA ATAGAAGACC AAAAGGTGAT GTCCCCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Smchd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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