AP2A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP2A1
Entrez Gene ID	784636
Full Name	adaptor related protein complex 2 alpha 1 subunit
General protein information	Preferred Names adaptor related protein complex 2 alpha 1 subunit Names AP-2 complex subunit alpha-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024978635.1	XP_024834403.1	AP-2 complex subunit alpha-1 isoform X1
XM_024978636.1	XP_024834404.1	AP-2 complex subunit alpha-1 isoform X2
NM_001191440.1	NP_001178369.1	AP-2 complex subunit alpha-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP2A1	XP_003953618.1
Xenopus tropicalis (tropical clawed frog)	ap2a1	NP_001090846.1
Homo sapiens (human)	AP2A1	NP_570603.2
Mus musculus (house mouse)	Ap2a1	NP_031484.1
Canis lupus familiaris (dog)	AP2A1	XP_003638863.1
Rattus norvegicus (Norway rat)	Ap2a1	NP_001100981.1
Danio rerio (zebrafish)	ap2a1	XP_005164119.1
Macaca mulatta (Rhesus monkey)	AP2A1	XP_001115452.1
Bos taurus (cattle)	AP2A1	NP_001178369.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AP2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01800	NM_001191440.1 Latest version!	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225232	XM_024978635.1 Latest version!	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225233	XM_024978636.1 Latest version!	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP2A1 ( NM_001191440.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178369.1 Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), mRNA.

Price & Availability↑

CloneID	OBa01800
Clone ID Related Accession (Same CDS sequence)	NM_001191440.1
Accession Version	NM_001191440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527868800000
Organism	Bos taurus(cattle)
Product	AP-2 complex subunit alpha-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000018.1, BT025356.1, DV913011.1, EH207219.1, DV895937.1, DV882337.1 and DT897608.1. On Apr 22, 2011 this sequence version replaced XM_002701929.1. Sequence Note: This RefSeq record was created from transcripts of different strains and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: SRR5571257.46967.1, SRR5571254.42947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145430 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAGGA GGCAGAGATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAGGGGGA CAAAGCCTTG GACGGCTACA GCAAGAAAAA GTATGTGTGT 
AAACTGCTCT TCATCTTCCT GCTTGGCCAC GACATTGACT TTGGGCACAT GGAGGCTGTG 
AACCTGCTGA GCTCCAACAA GTACACAGAG AAGCAGATTG GTTACCTGTT CATCTCGGTG 
CTGGTGAACT CCAACTCCGA GTTGATCCGG CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGCGAGATGG GAGAGGCCTT CGCCGCTGAC ATCCCTCGCA TCTTGGTGGC CGGGGACAGC 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCGA GCTGGAGCCG CCTGCCCCTG AGAGCCCCAT GGCTTTGCTG 
GCTGACCCAG CTCCAGCTGC TGACCCAGGT CCTGAGGACA TTGGCCCGCC CATCCCAGAA 
GCTGATGAGC TGCTGAATAA GTTCGTGTGC AGGAACAATG GGGTCCTGTT TGAGAACCAG 
CTGCTGCAGA TTGGAGTCAA GTCAGAGTTC CGGCAGAACT TGGGCCGCAT GTATCTCTTC 
TATGGCAACA AGACCTCGGT GCAGTTCCAG AACTTCTCTC CCACTGTGGT TCACCCTGGA 
GACCTCCAGA CTCAGCTGGC CGTGCAGACC AAGCGGGTGG CCTCGCAGGT GGACGGCGGG 
GCGCAGGTGC AGCAGGTGCT CAACATTGAG TGTCTGCGGG ACTTCCTGAC GCCGCCGCTC 
CTGTCGGTGC GCTTCCGGTA CGGGGGCGCC CCCCAGTCCC TCACCCTGAA ACTCCCAGTG 
ACCATCAACA AGTTCTTCCA GCCGACGGAG ATGGCGGCCC AGGACTTCTT CCAGCGTTGG 
AAGCAGCTGA GCCTCCCCCA ACAGGAGGCC CAGAAAATCT TCAAAGCCAA CCACCCCATG 
GACGCGGAGG TCACTAAGGC CAAGCTCCTG GGGTTTGGCT CTGCTCTCCT GGACAATGTG 
GACCCCAACC CTGAGAACTT CGTGGGGGCT GGAATCATCC AGACTAAAGC CCTGCAGGTG 
GGCTGTCTGC TCCGGCTGGA GCCCAATGCC CAGGCTCAGA TGTACCGGCT GACCCTGCGC 
ACCAGCAAGG AACCCGTCTC CCGTCACCTG TGTGAGCTGC TGGCGCAGCA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178369.1
CDS	88..3024
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDSRRDPSSHDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPSGAGNLLVDVFSDSPAAQPGLGPSPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCRNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVASQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_001191440.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191440.1

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAGGA GGCAGAGATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAGGGGGA CAAAGCCTTG GACGGCTACA GCAAGAAAAA GTATGTGTGT 
AAACTGCTCT TCATCTTCCT GCTTGGCCAC GACATTGACT TTGGGCACAT GGAGGCTGTG 
AACCTGCTGA GCTCCAACAA GTACACAGAG AAGCAGATTG GTTACCTGTT CATCTCGGTG 
CTGGTGAACT CCAACTCCGA GTTGATCCGG CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGCGAGATGG GAGAGGCCTT CGCCGCTGAC ATCCCTCGCA TCTTGGTGGC CGGGGACAGC 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCGA GCTGGAGCCG CCTGCCCCTG AGAGCCCCAT GGCTTTGCTG 
GCTGACCCAG CTCCAGCTGC TGACCCAGGT CCTGAGGACA TTGGCCCGCC CATCCCAGAA 
GCTGATGAGC TGCTGAATAA GTTCGTGTGC AGGAACAATG GGGTCCTGTT TGAGAACCAG 
CTGCTGCAGA TTGGAGTCAA GTCAGAGTTC CGGCAGAACT TGGGCCGCAT GTATCTCTTC 
TATGGCAACA AGACCTCGGT GCAGTTCCAG AACTTCTCTC CCACTGTGGT TCACCCTGGA 
GACCTCCAGA CTCAGCTGGC CGTGCAGACC AAGCGGGTGG CCTCGCAGGT GGACGGCGGG 
GCGCAGGTGC AGCAGGTGCT CAACATTGAG TGTCTGCGGG ACTTCCTGAC GCCGCCGCTC 
CTGTCGGTGC GCTTCCGGTA CGGGGGCGCC CCCCAGTCCC TCACCCTGAA ACTCCCAGTG 
ACCATCAACA AGTTCTTCCA GCCGACGGAG ATGGCGGCCC AGGACTTCTT CCAGCGTTGG 
AAGCAGCTGA GCCTCCCCCA ACAGGAGGCC CAGAAAATCT TCAAAGCCAA CCACCCCATG 
GACGCGGAGG TCACTAAGGC CAAGCTCCTG GGGTTTGGCT CTGCTCTCCT GGACAATGTG 
GACCCCAACC CTGAGAACTT CGTGGGGGCT GGAATCATCC AGACTAAAGC CCTGCAGGTG 
GGCTGTCTGC TCCGGCTGGA GCCCAATGCC CAGGCTCAGA TGTACCGGCT GACCCTGCGC 
ACCAGCAAGG AACCCGTCTC CCGTCACCTG TGTGAGCTGC TGGCGCAGCA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( XM_024978635.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834403.1 Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225232
Clone ID Related Accession (Same CDS sequence)	XM_024978635.1
Accession Version	XM_024978635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	AP-2 complex subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAGGA GGCAGAGATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAGGGGGA CAAAGCCTTG GACGGCTACA GCAAGAAAAA GTATGTGTGT 
AAACTGCTCT TCATCTTCCT GCTTGGCCAC GACATTGACT TTGGGCACAT GGAGGCTGTG 
AACCTGCTGA GCTCCAACAA GTACACAGAG AAGCAGATTG GTTACCTGTT CATCTCGGTG 
CTGGTGAACT CCAACTCCGA GTTGATCCGG CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGCGAGATGG GAGAGGCCTT CGCCGCTGAC ATCCCTCGCA TCTTGGTGGC CGGGGACAGC 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCCC AGGTCCTGAG GACATTGGCC CGCCCATCCC AGAAGCTGAT 
GAGCTGCTGA ATAAGTTCGT GTGCAGGAAC AATGGGGTCC TGTTTGAGAA CCAGCTGCTG 
CAGATTGGAG TCAAGTCAGA GTTCCGGCAG AACTTGGGCC GCATGTATCT CTTCTATGGC 
AACAAGACCT CGGTGCAGTT CCAGAACTTC TCTCCCACTG TGGTTCACCC TGGAGACCTC 
CAGACTCAGC TGGCCGTGCA GACCAAGCGG GTGGCCTCGC AGGTGGACGG CGGGGCGCAG 
GTGCAGCAGG TGCTCAACAT TGAGTGTCTG CGGGACTTCC TGACGCCGCC GCTCCTGTCG 
GTGCGCTTCC GGTACGGGGG CGCCCCCCAG TCCCTCACCC TGAAACTCCC AGTGACCATC 
AACAAGTTCT TCCAGCCGAC GGAGATGGCG GCCCAGGACT TCTTCCAGCG TTGGAAGCAG 
CTGAGCCTCC CCCAACAGGA GGCCCAGAAA ATCTTCAAAG CCAACCACCC CATGGACGCG 
GAGGTCACTA AGGCCAAGCT CCTGGGGTTT GGCTCTGCTC TCCTGGACAA TGTGGACCCC 
AACCCTGAGA ACTTCGTGGG GGCTGGAATC ATCCAGACTA AAGCCCTGCA GGTGGGCTGT 
CTGCTCCGGC TGGAGCCCAA TGCCCAGGCT CAGATGTACC GGCTGACCCT GCGCACCAGC 
AAGGAACCCG TCTCCCGTCA CCTGTGTGAG CTGCTGGCGC AGCAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834403.1
CDS	168..3038
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDSRRDPSSHDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPSGAGNLLVDVFSDSPAAQPGLGPSPEEAFLSPGPEDIGPPIPEADELLNKFVCRNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVASQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_024978635.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978635.1

ORF Insert Sequence:

ATGCCGGCCG TGTCCAAGGG CGATGGGATG CGGGGGCTCG CCGTGTTCAT CTCCGACATC 
CGGAACTGTA AGAGCAAGGA GGCAGAGATT AAGAGAATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAGGGGGA CAAAGCCTTG GACGGCTACA GCAAGAAAAA GTATGTGTGT 
AAACTGCTCT TCATCTTCCT GCTTGGCCAC GACATTGACT TTGGGCACAT GGAGGCTGTG 
AACCTGCTGA GCTCCAACAA GTACACAGAG AAGCAGATTG GTTACCTGTT CATCTCGGTG 
CTGGTGAACT CCAACTCCGA GTTGATCCGG CTCATCAACA ACGCCATCAA GAATGACCTG 
GCCAGCCGCA ACCCCACCTT CATGTGCCTG GCCCTGCACT GCATCGCCAA CGTGGGCAGC 
CGCGAGATGG GAGAGGCCTT CGCCGCTGAC ATCCCTCGCA TCTTGGTGGC CGGGGACAGC 
ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCCC AGGTCCTGAG GACATTGGCC CGCCCATCCC AGAAGCTGAT 
GAGCTGCTGA ATAAGTTCGT GTGCAGGAAC AATGGGGTCC TGTTTGAGAA CCAGCTGCTG 
CAGATTGGAG TCAAGTCAGA GTTCCGGCAG AACTTGGGCC GCATGTATCT CTTCTATGGC 
AACAAGACCT CGGTGCAGTT CCAGAACTTC TCTCCCACTG TGGTTCACCC TGGAGACCTC 
CAGACTCAGC TGGCCGTGCA GACCAAGCGG GTGGCCTCGC AGGTGGACGG CGGGGCGCAG 
GTGCAGCAGG TGCTCAACAT TGAGTGTCTG CGGGACTTCC TGACGCCGCC GCTCCTGTCG 
GTGCGCTTCC GGTACGGGGG CGCCCCCCAG TCCCTCACCC TGAAACTCCC AGTGACCATC 
AACAAGTTCT TCCAGCCGAC GGAGATGGCG GCCCAGGACT TCTTCCAGCG TTGGAAGCAG 
CTGAGCCTCC CCCAACAGGA GGCCCAGAAA ATCTTCAAAG CCAACCACCC CATGGACGCG 
GAGGTCACTA AGGCCAAGCT CCTGGGGTTT GGCTCTGCTC TCCTGGACAA TGTGGACCCC 
AACCCTGAGA ACTTCGTGGG GGCTGGAATC ATCCAGACTA AAGCCCTGCA GGTGGGCTGT 
CTGCTCCGGC TGGAGCCCAA TGCCCAGGCT CAGATGTACC GGCTGACCCT GCGCACCAGC 
AAGGAACCCG TCTCCCGTCA CCTGTGTGAG CTGCTGGCGC AGCAGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP2A1 ( XM_024978636.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834404.1 Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.

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CloneID	OBa225233
Clone ID Related Accession (Same CDS sequence)	XM_024978636.1
Accession Version	XM_024978636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	AP-2 complex subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCGA GCTGGAGCCG CCTGCCCCTG AGAGCCCCAT GGCTTTGCTG 
GCTGACCCAG CTCCAGCTGC TGACCCAGGT CCTGAGGACA TTGGCCCGCC CATCCCAGAA 
GCTGATGAGC TGCTGAATAA GTTCGTGTGC AGGAACAATG GGGTCCTGTT TGAGAACCAG 
CTGCTGCAGA TTGGAGTCAA GTCAGAGTTC CGGCAGAACT TGGGCCGCAT GTATCTCTTC 
TATGGCAACA AGACCTCGGT GCAGTTCCAG AACTTCTCTC CCACTGTGGT TCACCCTGGA 
GACCTCCAGA CTCAGCTGGC CGTGCAGACC AAGCGGGTGG CCTCGCAGGT GGACGGCGGG 
GCGCAGGTGC AGCAGGTGCT CAACATTGAG TGTCTGCGGG ACTTCCTGAC GCCGCCGCTC 
CTGTCGGTGC GCTTCCGGTA CGGGGGCGCC CCCCAGTCCC TCACCCTGAA ACTCCCAGTG 
ACCATCAACA AGTTCTTCCA GCCGACGGAG ATGGCGGCCC AGGACTTCTT CCAGCGTTGG 
AAGCAGCTGA GCCTCCCCCA ACAGGAGGCC CAGAAAATCT TCAAAGCCAA CCACCCCATG 
GACGCGGAGG TCACTAAGGC CAAGCTCCTG GGGTTTGGCT CTGCTCTCCT GGACAATGTG 
GACCCCAACC CTGAGAACTT CGTGGGGGCT GGAATCATCC AGACTAAAGC CCTGCAGGTG 
GGCTGTCTGC TCCGGCTGGA GCCCAATGCC CAGGCTCAGA TGTACCGGCT GACCCTGCGC 
ACCAGCAAGG AACCCGTCTC CCGTCACCTG TGTGAGCTGC TGGCGCAGCA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834404.1
CDS	937..3393
Translation	MDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDSRRDPSSHDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPSGAGNLLVDVFSDSPAAQPGLGPSPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCRNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVASQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_024978636.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adaptor related protein complex 2 subunit alpha 1 (AP2A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978636.1

ORF Insert Sequence:

ATGGACAGCG TGAAGCAGAG CGCGGCCCTG TGCCTGCTGC GGCTCTACAA GGCCTCGCCC 
GACCTGGTGC CCATGGGCGA GTGGACGGCC CGCGTGGTGC ACTTGCTCAA CGACCAGCAC 
ATGGGCGTGG TCACAGCCGC TGTCAGCCTC ATCACCTGTC TCTGCAAGAA GAACCCGGAT 
GACTTCAAGA CGTGCGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATCGT CTCCTCGGCC 
TCCACTGACC TCCAGGACTA CACCTACTAC TTCGTGCCAG CGCCCTGGCT CTCCGTGAAG 
CTCCTGCGGC TGCTGCAGTG CTACCCGCCA CCAGAGGACG CGGCCGTGAA GGGGCGGCTG 
GTGGAGTGCC TGGAGACCGT GCTCAACAAG GCCCAGGAGC CGCCCAAGTC CAAAAAGGTC 
CAGCACTCCA ACGCCAAGAA CGCCATCCTC TTCGAGACCA TCAGCCTCAT TATCCACTAC 
GACAGTGAGC CCAACCTCCT GGTTCGGGCC TGTAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGGGAGACCA ACCTGCGCTA CCTGGCCCTG GAGAGCATGT GCACGCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCGGTCAA GACGCACATC GACACCGTCA TCAACGCCCT CAAGACGGAG 
CGGGACGTCA GTGTGCGGCA GCGGGCGGCT GACCTCCTCT ACGCCATGTG TGATCGGAGC 
AACGCCAAGC AGATCGTGTC AGAGATGCTG CGGTACCTGG AAACGGCCGA CTACGCCATT 
CGTGAAGAGA TTGTCCTGAA GGTGGCCATC CTGGCTGAGA AGTACGCCGT GGACTACAGC 
TGGTACGTGG ACACCATCCT GAACCTCATC CGCATCGCGG GCGACTACGT GAGCGAGGAG 
GTGTGGTACC GCGTGCTGCA GATCGTCACC AACCGCGACG ACGTGCAGGG CTACGCTGCC 
AAGACCGTCT TCGAGGCGCT CCAGGCGCCC GCCTGCCATG AGAACATGGT GAAGGTCGGC 
GGCTACATCC TTGGGGAGTT TGGAAACCTG ATTGCTGGGG ACCCCCGCTC CAGCCCCCCG 
GTGCAGTTCT CCCTGCTGCA TTCCAAGTTC CACCTGTGCA GCGTGGCCAC GCGGGCCCTA 
CTGCTCTCCA CCTACATCAA GTTCATCAAC CTCTTCCCGG AAACCAAGGC CACCATCCAG 
GGCGTGCTGC GAGCCGGCTC CCAGCTGCGC AACGCCGACG TGGAGCTCCA GCAGCGGGCT 
GTCGAGTACC TCACCCTGAG CTCGGTGGCC AGCACGGACG TCCTGGCCAC GGTGCTGGAG 
GAGATGCCAC CCTTCCCCGA GCGTGAGTCA TCCATCCTGG CCAAGCTGAA GCGCAAGAAG 
GGGCCGGGGG CCGGCAGCGC CCTGGATGAC AGCCGGAGGG ACCCCAGCAG CCACGACATC 
AATGGGGGCG TGGAGCCCAC CCCCAGCACC GTTTCCACAC CCTCGCCCTC CGCTGACCTC 
CTGGGGCTGC GGGCAGCCCC TCCCCCCGCG GCACCCCCGG CCCCCTCGGG TGCAGGCAAC 
CTCCTGGTGG ACGTCTTCTC CGACAGCCCG GCCGCCCAGC CCGGCCTGGG GCCCAGCCCC 
GAGGAGGCCT TCCTCAGCGA GCTGGAGCCG CCTGCCCCTG AGAGCCCCAT GGCTTTGCTG 
GCTGACCCAG CTCCAGCTGC TGACCCAGGT CCTGAGGACA TTGGCCCGCC CATCCCAGAA 
GCTGATGAGC TGCTGAATAA GTTCGTGTGC AGGAACAATG GGGTCCTGTT TGAGAACCAG 
CTGCTGCAGA TTGGAGTCAA GTCAGAGTTC CGGCAGAACT TGGGCCGCAT GTATCTCTTC 
TATGGCAACA AGACCTCGGT GCAGTTCCAG AACTTCTCTC CCACTGTGGT TCACCCTGGA 
GACCTCCAGA CTCAGCTGGC CGTGCAGACC AAGCGGGTGG CCTCGCAGGT GGACGGCGGG 
GCGCAGGTGC AGCAGGTGCT CAACATTGAG TGTCTGCGGG ACTTCCTGAC GCCGCCGCTC 
CTGTCGGTGC GCTTCCGGTA CGGGGGCGCC CCCCAGTCCC TCACCCTGAA ACTCCCAGTG 
ACCATCAACA AGTTCTTCCA GCCGACGGAG ATGGCGGCCC AGGACTTCTT CCAGCGTTGG 
AAGCAGCTGA GCCTCCCCCA ACAGGAGGCC CAGAAAATCT TCAAAGCCAA CCACCCCATG 
GACGCGGAGG TCACTAAGGC CAAGCTCCTG GGGTTTGGCT CTGCTCTCCT GGACAATGTG 
GACCCCAACC CTGAGAACTT CGTGGGGGCT GGAATCATCC AGACTAAAGC CCTGCAGGTG 
GGCTGTCTGC TCCGGCTGGA GCCCAATGCC CAGGCTCAGA TGTACCGGCT GACCCTGCGC 
ACCAGCAAGG AACCCGTCTC CCGTCACCTG TGTGAGCTGC TGGCGCAGCA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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AP2A1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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