PPME1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPME1
Entrez Gene ID	535390
Full Name	protein phosphatase methylesterase 1
Synonyms	PME1MGC133824
General protein information	Preferred Names protein phosphatase methylesterase 1 Names protein phosphatase methylesterase 1 PME-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076056.2	NP_001069524.1	protein phosphatase methylesterase 1
XM_015474781.1	XP_015330267.1	protein phosphatase methylesterase 1 isoform X2
XM_005216225.2	XP_005216282.1	protein phosphatase methylesterase 1 isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D13046g	XP_453644.1
Rattus norvegicus (Norway rat)	Ppme1	NP_001178767.1
Danio rerio (zebrafish)	ppme1	NP_956231.1
Arabidopsis thaliana (thale cress)	AT4G10050	NP_567350.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008336	XP_317121.2
Schizosaccharomyces pombe (fission yeast)	SPBP4H10.17c	NP_596191.1
Magnaporthe oryzae (rice blast fungus)	MGG_15748	XP_003714633.1
Pan troglodytes (chimpanzee)	PPME1	XP_001174909.1
Bos taurus (cattle)	PPME1	NP_001069524.1
Drosophila melanogaster (fruit fly)	CG5068	NP_648277.3
Caenorhabditis elegans (roundworm)	B0464.9	NP_499084.1
Eremothecium gossypii	AGOS_AGR359C	NP_987025.1
Neurospora crassa	NCU00954	XP_965147.1
Xenopus tropicalis (tropical clawed frog)	ppme1	XP_002942566.1
Oryza sativa (rice)	Os04g0443500	NP_001052889.1
Saccharomyces cerevisiae (baker's yeast)	PPE1	NP_011942.1
Homo sapiens (human)	PPME1	NP_057231.1
Macaca mulatta (Rhesus monkey)	PPME1	XP_001115661.1
Canis lupus familiaris (dog)	PPME1	XP_005633576.1
Mus musculus (house mouse)	Ppme1	NP_082568.1
Gallus gallus (chicken)	PPME1	NP_001026005.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44957	NM_001076056.2 Latest version!	Bos taurus protein phosphatase methylesterase 1 (PPME1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa26573	XM_005216225.2 Latest version!	Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa92737	XM_015474781.1 Latest version!	Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPME1 ( NM_001076056.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069524.1 Bos taurus protein phosphatase methylesterase 1 (PPME1), mRNA.

Price & Availability↑

CloneID	OBa44957
Clone ID Related Accession (Same CDS sequence)	NM_001076056.2
Accession Version	NM_001076056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Bos taurus(cattle)
Product	protein phosphatase methylesterase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC110227.1. On Aug 30, 2012 this sequence version replaced NM_001076056.1. ##Evidence-Data-START## Transcript exon combination :: BC110227.1, BT021563.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACTTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGATGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCTA TTCCTAAATT GCTGCTCCTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACTATTGGCC AAATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TGCCCCAGTG TGGCCACGCA GTCCATGAGG ATGCTCCAGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATTCGGCAC AGGTTTGCAG AGCCTATCGG TGGATTCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069524.1
CDS	124..1266
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRGHGETKVRNSEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQ

Target ORF information:

RefSeq Version	NM_001076056.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase methylesterase 1 (PPME1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076056.2

ORF Insert Sequence:

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACTTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGATGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCTA TTCCTAAATT GCTGCTCCTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACTATTGGCC AAATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TGCCCCAGTG TGGCCACGCA GTCCATGAGG ATGCTCCAGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATTCGGCAC AGGTTTGCAG AGCCTATCGG TGGATTCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( XM_005216225.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216282.1 Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26573
Clone ID Related Accession (Same CDS sequence)	XM_005216225.2
Accession Version	XM_005216225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	protein phosphatase methylesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005216225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACTTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGATGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCTA TTCCTAAATT GCTGCTCCTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACTATTGGCC AAATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TGCCCCAGTG TGGCCACGCA GTCCATGAGG ATGCTCCAGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATTCGGCAC AGGTTTGCAG AGCCTATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216282.1
CDS	160..1320
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRGHGETKVRNSEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	XM_005216225.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216225.2

ORF Insert Sequence:

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACTTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGATGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCTA TTCCTAAATT GCTGCTCCTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACTATTGGCC AAATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TGCCCCAGTG TGGCCACGCA GTCCATGAGG ATGCTCCAGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATTCGGCAC AGGTTTGCAG AGCCTATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( XM_015474781.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330267.1 Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa92737
Clone ID Related Accession (Same CDS sequence)	XM_015474781.1
Accession Version	XM_015474781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	protein phosphatase methylesterase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGGTGATT 
TTTTTCCTGT TCCTCCTGGG ATTGCCCTCA GAGACTCAAA ACTTGCTCTT ACTCCTTCAG 
ACCAAGAAAG ACCATCCATA CACTTGGAGA ATTGAACTGG CAAAAACAGA AAAATACTGG 
GATGGCTGGT TCCGAGGCTT ATCCAATCTC TTTCTTAGTT GTCCTATTCC TAAATTGCTG 
CTCCTGGCTG GTGTTGATAG ATTGGATAAA GATCTGACTA TTGGCCAAAT GCAAGGGAAG 
TTCCAGATGC AGGTCCTGCC CCAGTGTGGC CACGCAGTCC ATGAGGATGC TCCAGACAAG 
GTAGCTGAAG CTGTTGCCAC TTTCCTGATT CGGCACAGGT TTGCAGAGCC TATCGGTGGA 
TTCCAGTGTG TGTTTCCTGG CTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330267.1
CDS	160..1386
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRGHGETKVRNSEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESVNKRKKEDDMEVIFFLFLLGLPSETQNLLLLLQTKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	XM_015474781.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474781.1

ORF Insert Sequence:

ATGTCGGCCC TCGAGAAGAG TATGCATCTT GGGCGCTTGC CCTCTCGCCC TCCACTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCTCCTG TTCCTTGGAG TCAGTACTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTTTAC AAGAGTGGTT CAGAAGGTCC AGTCCTACTC 
CTTCTGCATG GAGGAGGCCA TTCTGCCCTT TCCTGGGCTG TGTTCACGGC AGCAATCATT 
AGTAGAGTTC AATGTAGGAT TGTGGCTTTG GATCTTCGAG GTCATGGTGA AACAAAAGTC 
AGGAATTCTG AAGACCTGTC TGCAGAAACA ATGGCAAAAG ATGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTCCC TCCTCCAATT ATGCTCATTG GACATAGCAT GGGCGGTGCT 
ATTGCAGTCC ACACAGCATC GTCGAACCTG GTGCCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTGG AAGGTACAGC CATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGTGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCCATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GACGAAGAAG GAAGTGAGTC AGTAAACAAG AGGAAAAAGG AAGATGACAT GGAGGTGATT 
TTTTTCCTGT TCCTCCTGGG ATTGCCCTCA GAGACTCAAA ACTTGCTCTT ACTCCTTCAG 
ACCAAGAAAG ACCATCCATA CACTTGGAGA ATTGAACTGG CAAAAACAGA AAAATACTGG 
GATGGCTGGT TCCGAGGCTT ATCCAATCTC TTTCTTAGTT GTCCTATTCC TAAATTGCTG 
CTCCTGGCTG GTGTTGATAG ATTGGATAAA GATCTGACTA TTGGCCAAAT GCAAGGGAAG 
TTCCAGATGC AGGTCCTGCC CCAGTGTGGC CACGCAGTCC ATGAGGATGC TCCAGACAAG 
GTAGCTGAAG CTGTTGCCAC TTTCCTGATT CGGCACAGGT TTGCAGAGCC TATCGGTGGA 
TTCCAGTGTG TGTTTCCTGG CTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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PPME1 cDNA ORF clone, Bos taurus(cattle)

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