PPME1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPME1
Entrez Gene ID	51400
Full Name	protein phosphatase methylesterase 1
Synonyms	PME-1
General protein information	Preferred Names protein phosphatase methylesterase 1 Names protein phosphatase methylesterase 1 testicular secretory protein Li 39
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.4
Summary	This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012].
Disorder MIM:	611117

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271593.1	NP_001258522.1	protein phosphatase methylesterase 1 isoform b
NM_016147.2	NP_057231.1	protein phosphatase methylesterase 1 isoform a
NM_016147.3	NP_057231.1	protein phosphatase methylesterase 1 isoform a
NM_001271593.2	NP_001258522.1	protein phosphatase methylesterase 1 isoform b
XM_017017913.1	XP_016873402.1	protein phosphatase methylesterase 1 isoform X1
XM_017017913.2	XP_016873402.1	protein phosphatase methylesterase 1 isoform X1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D13046g	XP_453644.1
Rattus norvegicus (Norway rat)	Ppme1	NP_001178767.1
Danio rerio (zebrafish)	ppme1	NP_956231.1
Arabidopsis thaliana (thale cress)	AT4G10050	NP_567350.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008336	XP_317121.2
Schizosaccharomyces pombe (fission yeast)	SPBP4H10.17c	NP_596191.1
Magnaporthe oryzae (rice blast fungus)	MGG_15748	XP_003714633.1
Pan troglodytes (chimpanzee)	PPME1	XP_001174909.1
Bos taurus (cattle)	PPME1	NP_001069524.1
Drosophila melanogaster (fruit fly)	CG5068	NP_648277.3
Caenorhabditis elegans (roundworm)	B0464.9	NP_499084.1
Eremothecium gossypii	AGOS_AGR359C	NP_987025.1
Neurospora crassa	NCU00954	XP_965147.1
Xenopus tropicalis (tropical clawed frog)	ppme1	XP_002942566.1
Oryza sativa (rice)	Os04g0443500	NP_001052889.1
Saccharomyces cerevisiae (baker's yeast)	PPE1	NP_011942.1
Homo sapiens (human)	PPME1	NP_057231.1
Macaca mulatta (Rhesus monkey)	PPME1	XP_001115661.1
Canis lupus familiaris (dog)	PPME1	XP_005633576.1
Mus musculus (house mouse)	Ppme1	NP_082568.1
Gallus gallus (chicken)	PPME1	NP_001026005.1

Gene Ontology
Bibliography

Related articles in PubMed

Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1.
Tang S, Lu C, Mo L, Wang X, Liang Z, Qin F, Liu Y, Liu Y, Huang H, Huang Y, Cai H, Xiao D, Guo S, Ouyang Y, Sun B, Li X
Life sciences213166-173(2018 Nov)

PME-1 is regulated by USP36 in ERK and Akt signaling pathways.
Kim SY, Choi J, Lee DH, Park JH, Hwang YJ, Baek KH
FEBS letters592(9)1575-1588(2018 05)

NNMT Silencing Activates Tumor Suppressor PP2A, Inactivates Oncogenic STKs, and Inhibits Tumor Forming Ability.
Palanichamy K, Kanji S, Gordon N, Thirumoorthy K, Jacob JR, Litzenberg KT, Patel D, Chakravarti A
Clinical cancer research : an official journal of the American Association for Cancer Research23(9)2325-2334(2017 May)

Regulation of protein phosphatase 2A (PP2A) tumor suppressor function by PME-1.
Kaur A, Westermarck J
Biochemical Society transactions44(6)1683-1693(2016 12)

Inhibition of protein methylesterase 1 decreased cancerous phenotypes in endometrial adenocarcinoma cell lines and xenograft tumor models.
Pusey M, Bail S, Xu Y, Buiakova O, Nestor M, Yang JJ, Rice LM
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(9)11835-11842(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu92312	XM_017017913.2 Latest version!	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu00409	NM_016147.3 Latest version!	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu03839	NM_001271593.2 Latest version!	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu00409	NM_016147.2	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu03839	NM_001271593.1	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu92312	XM_017017913.1	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPME1 ( XM_017017913.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873402.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92312
Clone ID Related Accession (Same CDS sequence)	XM_017017913.1 , XM_017017913.2
Accession Version	XM_017017913.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873402.1
CDS	532..1368
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDME

Target ORF information:

RefSeq Version	XM_017017913.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017913.1

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( XM_017017913.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873402.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu92312
Clone ID Related Accession (Same CDS sequence)	XM_017017913.1 , XM_017017913.2
Accession Version	XM_017017913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873402.1
CDS	333..1169
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDME

Target ORF information:

RefSeq Version	XM_017017913.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017913.2

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( NM_016147.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057231.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00409
Clone ID Related Accession (Same CDS sequence)	NM_016147.2 , NM_016147.3
Accession Version	NM_016147.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA824678.1, DA691258.1, AK022725.1 and AF157028.1. On Oct 24, 2012 this sequence version replaced NM_016147.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF157028.1, SRR1803617.212200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACCTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGACGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCCA TTCCTAAATT GCTGCTCTTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACCATTGGCC AGATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AACCCATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057231.1
CDS	360..1520
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	NM_016147.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016147.2

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACCTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGACGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCCA TTCCTAAATT GCTGCTCTTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACCATTGGCC AGATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AACCCATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( NM_016147.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057231.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00409
Clone ID Related Accession (Same CDS sequence)	NM_016147.2 , NM_016147.3
Accession Version	NM_016147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562256000000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK022725.1 and AF157028.1. On Nov 23, 2018 this sequence version replaced NM_016147.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.599365.1, SRR1803614.153902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328257.13/ ENSP00000329867.8 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACCTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGACGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCCA TTCCTAAATT GCTGCTCTTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACCATTGGCC AGATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AACCCATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057231.1
CDS	134..1294
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	NM_016147.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016147.3

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGACCAAG 
AAAGACCATC CATACACCTG GAGAATTGAA CTGGCAAAAA CAGAAAAATA CTGGGACGGC 
TGGTTCCGAG GCTTATCCAA TCTCTTTCTT AGTTGTCCCA TTCCTAAATT GCTGCTCTTG 
GCTGGTGTTG ATAGATTGGA TAAAGATCTG ACCATTGGCC AGATGCAAGG GAAGTTCCAG 
ATGCAGGTCC TACCCCAGTG TGGCCATGCA GTCCATGAGG ATGCCCCTGA CAAGGTAGCT 
GAAGCTGTTG CCACTTTCCT GATCCGGCAC AGGTTTGCAG AACCCATCGG TGGATTCCAG 
TGTGTGTTTC CTGGCTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( NM_001271593.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258522.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03839
Clone ID Related Accession (Same CDS sequence)	NM_001271593.1 , NM_001271593.2
Accession Version	NM_001271593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551542400000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA824678.1, AK225647.1 and BC050705.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. It encodes a longer protein (isoform b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225647.1, SRR1803616.276513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGGGGTTG 
CCCTCGGAGA CTCAGAACTT GCTCTTATTC CTCCAGACCA AGAAAGACCA TCCATACACC 
TGGAGAATTG AACTGGCAAA AACAGAAAAA TACTGGGACG GCTGGTTCCG AGGCTTATCC 
AATCTCTTTC TTAGTTGTCC CATTCCTAAA TTGCTGCTCT TGGCTGGTGT TGATAGATTG 
GATAAAGATC TGACCATTGG CCAGATGCAA GGGAAGTTCC AGATGCAGGT CCTACCCCAG 
TGTGGCCATG CAGTCCATGA GGATGCCCCT GACAAGGTAG CTGAAGCTGT TGCCACTTTC 
CTGATCCGGC ACAGGTTTGC AGAACCCATC GGTGGATTCC AGTGTGTGTT TCCTGGCTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258522.1
CDS	360..1562
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMEGLPSETQNLLLFLQTKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	NM_001271593.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271593.1

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGGGGTTG 
CCCTCGGAGA CTCAGAACTT GCTCTTATTC CTCCAGACCA AGAAAGACCA TCCATACACC 
TGGAGAATTG AACTGGCAAA AACAGAAAAA TACTGGGACG GCTGGTTCCG AGGCTTATCC 
AATCTCTTTC TTAGTTGTCC CATTCCTAAA TTGCTGCTCT TGGCTGGTGT TGATAGATTG 
GATAAAGATC TGACCATTGG CCAGATGCAA GGGAAGTTCC AGATGCAGGT CCTACCCCAG 
TGTGGCCATG CAGTCCATGA GGATGCCCCT GACAAGGTAG CTGAAGCTGT TGCCACTTTC 
CTGATCCGGC ACAGGTTTGC AGAACCCATC GGTGGATTCC AGTGTGTGTT TCCTGGCTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPME1 ( NM_001271593.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258522.1 Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03839
Clone ID Related Accession (Same CDS sequence)	NM_001271593.1 , NM_001271593.2
Accession Version	NM_001271593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	protein phosphatase methylesterase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA824678.1, AK225647.1 and BC050705.2. On May 31, 2019 this sequence version replaced NM_001271593.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. It encodes a longer protein (isoform b) compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.276513.1, AK225647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGGGGTTG 
CCCTCGGAGA CTCAGAACTT GCTCTTATTC CTCCAGACCA AGAAAGACCA TCCATACACC 
TGGAGAATTG AACTGGCAAA AACAGAAAAA TACTGGGACG GCTGGTTCCG AGGCTTATCC 
AATCTCTTTC TTAGTTGTCC CATTCCTAAA TTGCTGCTCT TGGCTGGTGT TGATAGATTG 
GATAAAGATC TGACCATTGG CCAGATGCAA GGGAAGTTCC AGATGCAGGT CCTACCCCAG 
TGTGGCCATG CAGTCCATGA GGATGCCCCT GACAAGGTAG CTGAAGCTGT TGCCACTTTC 
CTGATCCGGC ACAGGTTTGC AGAACCCATC GGTGGATTCC AGTGTGTGTT TCCTGGCTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258522.1
CDS	134..1336
Translation	MSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMEGLPSETQNLLLFLQTKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC

Target ORF information:

RefSeq Version	NM_001271593.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271593.2

ORF Insert Sequence:

ATGTCGGCCC TCGAAAAGAG CATGCACCTC GGCCGCCTTC CCTCTCGCCC ACCTCTACCC 
GGCAGCGGGG GCAGTCAGAG CGGAGCCAAG ATGCGAATGG GCCCTGGAAG AAAGCGGGAC 
TTTTCCCCTG TTCCTTGGAG TCAGTATTTT GAGTCCATGG AAGATGTAGA AGTAGAGAAT 
GAAACTGGCA AGGATACTTT TCGAGTCTAC AAGAGTGGTT CAGAGGGTCC AGTCCTGCTC 
CTTCTGCATG GAGGAGGTCA TTCTGCCCTT TCTTGGGCTG TGTTCACGGC AGCGATTATT 
AGTAGAGTTC AGTGTAGGAT TGTAGCTTTG GATCTGCGAA GTCATGGTGA AACAAAGGTC 
AAGAATCCTG AAGATCTGTC TGCAGAAACA ATGGCAAAAG ACGTTGGCAA TGTGGTTGAA 
GCCATGTATG GGGACCTTCC TCCTCCAATT ATGCTGATTG GACATAGCAT GGGTGGTGCT 
ATTGCAGTCC ACACAGCATC ATCCAACCTG GTACCAAGCC TCTTGGGTCT GTGCATGATT 
GATGTTGTAG AAGGTACAGC TATGGATGCA CTTAATAGCA TGCAGAATTT CTTACGGGGT 
CGTCCTAAAA CCTTCAAGTC TCTGGAGAAT GCTATTGAAT GGAGTGTGAA GAGTGGCCAG 
ATTCGAAATC TGGAGTCTGC CCGTGTCTCA ATGGTTGGCC AAGTCAAACA GTGTGAAGGA 
ATTACAAGTC CAGAAGGCTC AAAATCTATA GTGGAAGGAA TCATAGAGGA AGAAGAAGAA 
GATGAGGAAG GAAGTGAGTC TATAAGCAAG AGGAAAAAGG AAGATGACAT GGAGGGGTTG 
CCCTCGGAGA CTCAGAACTT GCTCTTATTC CTCCAGACCA AGAAAGACCA TCCATACACC 
TGGAGAATTG AACTGGCAAA AACAGAAAAA TACTGGGACG GCTGGTTCCG AGGCTTATCC 
AATCTCTTTC TTAGTTGTCC CATTCCTAAA TTGCTGCTCT TGGCTGGTGT TGATAGATTG 
GATAAAGATC TGACCATTGG CCAGATGCAA GGGAAGTTCC AGATGCAGGT CCTACCCCAG 
TGTGGCCATG CAGTCCATGA GGATGCCCCT GACAAGGTAG CTGAAGCTGT TGCCACTTTC 
CTGATCCGGC ACAGGTTTGC AGAACCCATC GGTGGATTCC AGTGTGTGTT TCCTGGCTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hydrogen peroxide redistributes the localization of protein phosphatase methylesterase 1.
Life sciences213166-173(2018 Nov)
Tang S,Lu C,Mo L,Wang X,Liang Z,Qin F,Liu Y,Liu Y,Huang H,Huang Y,Cai H,Xiao D,Guo S,Ouyang Y,Sun B,Li X

PME-1 is regulated by USP36 in ERK and Akt signaling pathways.
FEBS letters592(9)1575-1588(2018 05)
Kim SY,Choi J,Lee DH,Park JH,Hwang YJ,Baek KH

NNMT Silencing Activates Tumor Suppressor PP2A, Inactivates Oncogenic STKs, and Inhibits Tumor Forming Ability.
Clinical cancer research : an official journal of the American Association for Cancer Research23(9)2325-2334(2017 May)
Palanichamy K,Kanji S,Gordon N,Thirumoorthy K,Jacob JR,Litzenberg KT,Patel D,Chakravarti A

Regulation of protein phosphatase 2A (PP2A) tumor suppressor function by PME-1.
Biochemical Society transactions44(6)1683-1693(2016 12)
Kaur A,Westermarck J

Inhibition of protein methylesterase 1 decreased cancerous phenotypes in endometrial adenocarcinoma cell lines and xenograft tumor models.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(9)11835-11842(2016 Sep)
Pusey M,Bail S,Xu Y,Buiakova O,Nestor M,Yang JJ,Rice LM

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

PPME1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM