pole cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pole
Entrez Gene ID	553405
Full Name	polymerase (DNA directed), epsilon
Synonyms	si:dkeyp-98c11.2
General protein information	Preferred Names polymerase (DNA directed), epsilon Names DNA polymerase epsilon catalytic subunit A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128523.1	NP_001121995.1	DNA polymerase epsilon catalytic subunit A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development.
Iwanami N, Sikora K, Richter AS, Mönnich M, Guerri L, Soza-Ried C, Lawir DF, Mateos F, Hess I, O'Meara CP, Schorpp M, Boehm T
Cell reports17(9)2259-2270(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pole gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pole cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32699	NM_001128523.1 Latest version!	Danio rerio polymerase (DNA directed), epsilon (pole), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pole ( NM_001128523.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121995.1 Danio rerio polymerase (DNA directed), epsilon (pole), mRNA.

Price & Availability↑

CloneID	ODa32699
Clone ID Related Accession (Same CDS sequence)	NM_001128523.1
Accession Version	NM_001128523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1499184000000
Organism	Danio rerio(zebrafish)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT737234.14. On Jul 31, 2008 this sequence version replaced XM_001920387.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01030875.1, GDQH01021551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476756, SAMEA4476771 [ECO:0000348] ##Evidence-Data-END##

ATGGTTTTAC AAAACAGTGG ACGATATAAA GCTGACCGAG GCGGAGATCA AGACAACCAG 
CAGGATGATG CGTCAGCTTT GTCTGCTGTC AAGCGCCTGG AGCGCAGTCA GTTCACTGAC 
AGTATGGACG AGCGTTTCGG CTTTGAACGG ATGAAGGAGC CTGGGGAGAA AACAGGATGG 
CTTATCAACA TGCACCCGAC TGAGATTCTG GATGATGACA AACGAATGAT CAGTGCTGTG 
GACTATTACT TCATACAGGA AGATGGAAAC AGATTCAAGG TTGCTCTTCC CTACAAGCCA 
TATTTCTATA TTGCCACTAA AAAGAACACT GAAAGAGAAG TGATCTCCTT CCTGTCAAAG 
AAGTTCCAAG GGAAAGTAGC GAAGCTTGAA TCTGTGCCAA AGGAAGATTT AGATCTGCCT 
AACCACCTGG TCGGACTAAA GAGGAACTAC ATCAAACTCT CTTTTAACAC TGTGGATGAT 
CTTATTAAAG TTAAAAGAGA GATCTCACCT GCTGTCCGCA AGAACAGGGA GAGAGAGAAG 
TCTAATGATG CCTATACAAG CATGCTGTCC AGCGCTTTGG TTGGTGGGAG TGTAGTGACG 
GAGGATGAGG GTGGATCGTC AAAAAAGATG ACCGAGCAGC TAGACAACAT CCTAGACATG 
CGGGAATATG ATGTGCCCTA TCACGTCCGC GTTTCTATTG ACTTAAAGAT TCACGTGGCT 
CACTGGTATA ATGTGCGGTA TAGAGGAAGT GCTTATCCTC CTGAGATTGT GCGCAGGGAC 
GACCTTGTGG AAAGACCTGA TCCTGTTGTG TTGGCTTTTG ATATTGAGAC CACTAAACTT 
CCTCTGAAGT TTCCTGATGC TGAAACAGAC CAGATCATGA TGATATCATA CATGATAGAT 
GGACAGGGTT TCCTCATCAC AAACAGAGAA ATTGTGTCTG AGGACATTGA AGACTTTGAG 
TTCACCCCAA AACCTGAATA TGAGGGCCCT TTCACTATAT TTAATGAGCC TGATGAGGCC 
TCTCTCATTC AGAGATGGTT TGAGCACGTC CATGAGACCA AGCCAAATAT TTTTGTCACC 
TACAACGGGG ATTTCTTTGA CTGGCCATTT GTAGAAGCCA GAGCTGCCCA GTTAGGTCTG 
AGTATGCATC GGGAGATCGG CTTCCAGAAG GACAATCAGG GAGAATATAA AGCCAGCCAG 
GCTATTCACA TGGACTGCCT CAGGTGGGTA AAGAGAGACA GTTACCTGCC TGTAGGAAGT 
CATAACCTCA AGGCTGCAGC AAAAGCTAAA TTAGGCTATG ACCCTGTAGA GCTGGACCCA 
GAGGAGATGT GCCGCATGGC CACTGAAGAA CCACAGACCC TGGCCACCTA TTCAGTGTCT 
GACGCTGTGG CTACATACTA CCTCTACATG AAATATGTTC ATCCCTTCAT TTTTGCCCTC 
TGCACTATTA TCCCTATGGA ACCTGATGAG GTTTTGCGGA AAGGCTCCGG TACCTTGTGC 
GAAGCCTTGT TGATGGTTCA GGCCTACCAT GTCAACATCG TCTTCCCAAA CAAGCAGGAG 
CAGGTCTTTA ACAAACTCAC AGATGATGGG CATGTATTGG ACTCTGAGAC ATATGTGGGC 
GGCCACGTCG AGGCTCTGGA GTCTGGTGTG TTTCGCAGTG ACATCCCCTG CCGCTTTAAA 
ATGAATCCTG CAGCGTTTGA CTTTTTGATT CAGAGGGTGG AACGTACTCT TCGTCATGCA 
ATTGAGGAAG AGGAGAAGAT TCCTCTAGAG CAGGTTACCA ACTTTAATGA GGTGTGTGAT 
GAGATCAAGA GGAAGCTGAT CTCTTTGAAG GAAGTGCCCA ACAGAATCGA GTGTCCTCTT 
ATTTACCATC TTGATGTAGG GGCCATGTAC CCCAACATCA TCCTCACCAA CAGACTACAG 
CCTTCTGCCA TGGTTGATGA GGCGACCTGT GCCGCCTGTG ATTTTAATAA ACCTGGGGCA 
AACTGTCAGC GCAGAATGAC TTGGCAATGG AGAGGAGAGA TCATGCCAGC AAGTAGGAGT 
GAGTTTCATC GCATTCAGCA GCAACTGGAG TCCGAGAAGT TTCCCCCTGT TTTTCCTAAT 
GGTCCACCTC GTGCTTTCCA TGAGCTCAAT AGAGAAGAAC AGGCTCGGCA TGAGAAGAAG 
CGTTTAGGAG ACTACTGTAG AAAAGCCTAT AAGAAGGTTC ACCTGACCAG GCTGGAGGAG 
AGAGTCACTA CTATCTGCCA GAGAGAAAAC TCCTTTTATG TTGACACAGT TCGAGCCTTC 
AGAGACCGTC GATATGAGTT TAAAGGACTC CACAAGGTGT GGAAGAAAAA GCTGTCTACA 
GCACAGGATA ATGGGGACGC AGCGGAGGTG AAGCGCTGCA AGAACATGGA GATTCTTTAT 
GAGTCTCTTC AGCTGGCCCA CAAGTGCATC CTCAACTCCT TTTACGGATA CGTCATGAGA 
AAAGGAGCAC GTTGGTACTC AATGGAGATG GCTGGGATTG TTTGTTACAC CGGTGCCAAC 
ATCATCACAC AGGCCAGAGA ACTCATCGAG CAGATTGGGA GGCCTCTGGA GTTGGACACT 
GATGGTATAT GGTGTGTTCT TCCCAATACC TTTCCTGAGA ATTTTGTCAT CAAATCCACC 
AATGAGAAGA AGCCAAAAGT GACCATTAGT TATCCTGGAG CCATGCTCAA TATTATGGTC 
AAGGAGGGCT TCACAAATCA TCAGTATCAA GAGTTGGTGG ACGCCGCATC TCTTACCTAT 
GAGACCAGAG CTGAGAACAG TATCTTCTTT GAAGTTGATG GCCCGTACTT GGCTATGATC 
CTGCCTGCCT CCAAAGAAGA GGGAAAGAAA CTAAAGAAAA GATATGCTGT GTTCAATGAG 
GACGGCTCTC TGGCAGAGCT GAAGGGGTTT GAAGTGAAGA GGAGAGGAGA ACTTCAGCTC 
ATTAAGATCT TCCAGTCGTC TGTGTTCGAG GCGTTCTTGA AAGGCACAAC TCTGGAAGAG 
GTTTACGCCT CTGTGGCTAA AGTGGCTGAC TACTGGCTTG ATGTGCTCTA CAGCAAGGCT 
GCTAACATGC CAGATGCAGA GTTGTTTGAG CTGATTTCGG AAAACCGTTC GATGTCTCGT 
AAACTGGAAG ATTATGGTGA ACAGAAATCC ACCTCGATTA GCACCGCCAA ACGTCTTGCT 
GAGTTTCTTG GCGATCAGAT GGTGAAGGAC GCCGGCCTCA GCTGTCGATA TGTCATCTCC 
CGCAAACCAG AGGGCTCGCC TGTCACTGAG AGGGCAATCC CGCTAGCCAT TTTTCAGGCT 
GAAGAGAGTG TTAAAAAGCA TTTTCTACGG AAATGGCTGA AGATGCCCAG TCTCCATGAC 
CTTGACATTA GATCTATTCT GGACTGGAGT TACTACATTG AGCGTTTGGG CAGTGCTATT 
CAGAAAATCA TCACAATCCC CGCGGCTCTA CAACAAGTTA AAAACCCAGT GCCCAGAGTA 
CGCCATCCAG ACTGGCTGCA CAAGAAACTT CTGGAAAAGA ATGACATCTA CAAGCAGAAG 
AAGATTAGCG AGCTCTTCAC CAGTGAGGGA AAGAGACAGG TGGCCCAACA AACTCTAGCA 
GCAGGCGAGA CTCCAGATAT GGAGGATTTG GCTGCTCCTC AGCGCCCCGT GCAGCCTGCT 
ATCCTCATCA GCACAAAGAG GAAGAGGATC TCTCAAGGAG AGGACAGTCA GACTGAGACT 
CAAGAGCAAG AACTCACACA GTCTTGGAGA GAAATACTGG GCCCACCACC GGCCATGGGT 
GCCACCCGGG AGGAAATATT AGTCTGGTTG CGCTACCATA AGAAAAAATG GGAGCTGCAG 
CTTCGGCAGA GAAAGGAACG TCGGAAGCGG CGGCGTTTGA TTGATGGAGA ATCTCAGCCG 
ACTGGAGGCG GTGTTATCCG TGGGGGACCC ACAACAGGGC TTGGCAGCTT CCTGCGGAGG 
ACCGCTCGCA GTATTCTAGA CATGCCTTGG CAGATTGTAC AGATTGCAGA GACCAGTCAC 
CCAGGCCTTT ATAAGCTGTG GGCCGTCATT GGCAGTGACC TCCACTGCAT GAAACTCAAC 
ATCCCTCGAG TGTTTTATGT CAACCAGAGA GTGCCTAAAC AAGAAGAGGC TGCTACCTGC 
AAAAAGGTGA ACCGGATCCT TCCTCGCTCT GGTGTGGCGT GTTTCCTGTA CCAGTATACT 
GTTCCTGAGG ACATGTACCA GGAACACATC AATGAAATCA ATGCTGACCT CTCGGCTCCT 
GATATTGAGG GCGTCTATGA GACACAGGTT CCTTTACTGT TCCGGGCTTT GGTCCAGCTG 
GGCTGCATGT GTATGGTCAA TAAACATGTG GTCAGAGACC TGGCTGGCAG AGAGGCGGAT 
ACCTTTGACC TGGAGCACCT AGAGATGAGA TCCTTGGCCC AGTTTAGCTA CCTTGAGCCA 
GGCAGTGTAA GGCACATCTA CCTGTACCAT CACAGTCAGG GTCATAAGGC TCTCTTTGGC 
CTCTTCATAC CATCGCAACG CAAAGCCAGC ATCTTTGTTC TGGACACGGT CCGCAGTAAT 
CAGATGCCCA ACCTGAACTC ACTGTATGGA GCCGAGCGCA CAGCACTGCT GGAGAAAACC 
ACAGAGGAGC TTCTGCCACC CGAGAAACAT CTGTTTGAAG TGCGTGCTGA AAATGACATC 
AAAGCCATCT ACCGTGCACT GCAGCGCATC CTCCTCAACT ACAAGGAGGA GCGCCGGGGT 
CCAACCCTGA TTGCTGTTCA GTCCAACTGG GAGCTGCGGC GTCTGGCGGC CGGTATGACT 
GTACTGGAGG AGTTCCCGGT GGTGCCAGTT CACGTTATAG ATGAGATCAG CTATAATGTA 
CTGGACTGGC AAAGGCACGG AGCTCGACGC ATGATTAAAC ACTACCTGAA CCTGGACAGC 
TGCCTCTCAC AGGCTTTTGA TATGGCCAGG TATTATCACC TGCCTGTAGG AAACCTGCCT 
CAGGACATTT CTATATTTGG ATCGGATCTA TTTCTTGCAC GTCATCTGAG AAAGCACAAC 
CACCTGTTGT GGCTCTCACC CACGGCACGG CCGGACCTCG GGGGAAAAGA GGCTGACGAC 
AGCAGGCTGG TGATGGAGAG TGATGAGAGA GGTTCCATGG AAATTAATAG CCAGGGCTGC 
TACTCCACAG TTTGTGTGGA GCTGGACATT CAGAGTTTGG CGGTAAACAC CATTCTGCAG 
TCTCAGCATG TAAACGACAT GGAGGGCGGA GCCAGCATTG GGGTCAGCTT TGATGTCATT 
CAGCACGCAT CGCTGGAGGA GATGATGTCA GGGAATCAGG GGGCGAATGC GGTGGCCAGC 
TATGATGAGA CTGCACTCTG CTCCAACACA TTCAGGATTC TGAAGAGTAT GGTTGTTGGT 
TGGGTTCGAG AGATCACACA GTATCATAAC GTCTATGCTG ATAACCAGGT GATGCATTTT 
TATCGGTGGT TGCGCTCACC CAGTTCTCTA CTATATGACC CGGCTCTGCA CCGCACGCTG 
CTTAACATGA TGAAGAAGAT CTTTCTACAA CTCGTGGCAG AGTTTAAACG CTTGGGATCA 
TCTGTGATCT ATGGAAACTT CAACCGTCTC ATCCTCTGCA CCAAGAAACG ACGCATTGAT 
GACGCCATTG CATATGTGGA GTACATCACT AACAGTATCC ACACGAAGGA GATCTTCCAC 
TCTCTGTCCA TCACATTTTC CCGCTGTTGG GAGTTTCTGC TGTGGATGGA TCCTGCTAAC 
TGTGGAGGAG TGAAGGGAAA GCTGCCCTCC AGCCTTCTGT ATGGAGAGCA TGACAGTGAA 
AAGAAGAAGA AAGATGGAGC AGAAGAGGAT GGAAGTGATG ATGAAGATGA ACATGAAGAA 
AACCAAGAGG AAGATGAAGA ACAGGACAGG GAGGAAGACG TGGAGGATCT GATTGAAAGT 
AACTGGAACA TAATGCAGTA TCTTCCTCAG ACGGCCTCCT GTCAGAAATA CTTTCTCATG 
ATCATTTCAG CCTACATCGC AGCTGTTTAT CACAGCATGA GACAAGAACT TCGGCGAAAC 
GCCCCTGGAG CCACTCCCAT TAAGAGACGA GGAGCAACAC AGGCATCCCA GCAGCCATCT 
GGAGATGTCA GTGCGCTTCC TGGTTTGATT ACATTCTCCC AGGAGTATGT GTCAAGTGAA 
CTCACACAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTGTC GAGCACTCGC 
AGTGTTACGC TCCCGTCTGA GATGTTCCCT GTTCTTCCTG GTTCTCATTT GCCGCTCAAC 
AACCCTGCGC TGGAGTTCAT CAAATACGTC TGCCAGGTTC TCTCTCTGGA CGCCAACATT 
GTCAATCAGG TGAACAAACT AAAGCGAGAT CTTCTGCGCT TGGTGGATGT TGGAGAGTTT 
TCAGAAGACG CCCAGTTTCG TGACCCCTGC AAGTCATATG TCCTGCCAGA GGTCATCTGT 
CACCACTGCA ACTTCTGTCG AGACCTGGAC CTCTGTAAAG ACCCTGCAGT TTCACAGGAT 
GGTTCAGTTC TTCCACAATG GTTCTGTTCT AACTGTCAGG CTCAGTACGA CACTGAGTCT 
ATAGAAATGG CTCTGGTGGA GGCCCTGCAG AAGAAACTAA TGAGCTACAC GCTGCAAGAT 
GTGGAGTGTG CCAAATGCAA AGGAGTGAAG GAGGCGAACA TGCCGCTGTA CTGTAGCTGT 
GCTGGAGATT TCAACCTCAC CTTCACCACA AAGAGCTTCT CTGAGCAGGT GGAAGTTTTC 
AGAAACATCG CTGCTCATTA TAATATGAGC TTCTTATTGG AGACCATTGA CTGGATCATC 
AGTATGAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121995.1
CDS	146..7000
Translation	MVLQNSGRYKADRGGDQDNQQDDASALSAVKRLERSQFTDSMDERFGFERMKEPGEKTGWLINMHPTEILDDDKRMISAVDYYFIQEDGNRFKVALPYKPYFYIATKKNTEREVISFLSKKFQGKVAKLESVPKEDLDLPNHLVGLKRNYIKLSFNTVDDLIKVKREISPAVRKNREREKSNDAYTSMLSSALVGGSVVTEDEGGSSKKMTEQLDNILDMREYDVPYHVRVSIDLKIHVAHWYNVRYRGSAYPPEIVRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGFLITNREIVSEDIEDFEFTPKPEYEGPFTIFNEPDEASLIQRWFEHVHETKPNIFVTYNGDFFDWPFVEARAAQLGLSMHREIGFQKDNQGEYKASQAIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEEMCRMATEEPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAYHVNIVFPNKQEQVFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFKMNPAAFDFLIQRVERTLRHAIEEEEKIPLEQVTNFNEVCDEIKRKLISLKEVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRRMTWQWRGEIMPASRSEFHRIQQQLESEKFPPVFPNGPPRAFHELNREEQARHEKKRLGDYCRKAYKKVHLTRLEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSTAQDNGDAAEVKRCKNMEILYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYTGANIITQARELIEQIGRPLELDTDGIWCVLPNTFPENFVIKSTNEKKPKVTISYPGAMLNIMVKEGFTNHQYQELVDAASLTYETRAENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGTTLEEVYASVAKVADYWLDVLYSKAANMPDAELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYVISRKPEGSPVTERAIPLAIFQAEESVKKHFLRKWLKMPSLHDLDIRSILDWSYYIERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLEKNDIYKQKKISELFTSEGKRQVAQQTLAAGETPDMEDLAAPQRPVQPAILISTKRKRISQGEDSQTETQEQELTQSWREILGPPPAMGATREEILVWLRYHKKKWELQLRQRKERRKRRRLIDGESQPTGGGVIRGGPTTGLGSFLRRTARSILDMPWQIVQIAETSHPGLYKLWAVIGSDLHCMKLNIPRVFYVNQRVPKQEEAATCKKVNRILPRSGVACFLYQYTVPEDMYQEHINEINADLSAPDIEGVYETQVPLLFRALVQLGCMCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHIYLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLNSLYGAERTALLEKTTEELLPPEKHLFEVRAENDIKAIYRALQRILLNYKEERRGPTLIAVQSNWELRRLAAGMTVLEEFPVVPVHVIDEISYNVLDWQRHGARRMIKHYLNLDSCLSQAFDMARYYHLPVGNLPQDISIFGSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDERGSMEINSQGCYSTVCVELDIQSLAVNTILQSQHVNDMEGGASIGVSFDVIQHASLEEMMSGNQGANAVASYDETALCSNTFRILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLNMMKKIFLQLVAEFKRLGSSVIYGNFNRLILCTKKRRIDDAIAYVEYITNSIHTKEIFHSLSITFSRCWEFLLWMDPANCGGVKGKLPSSLLYGEHDSEKKKKDGAEEDGSDDEDEHEENQEEDEEQDREEDVEDLIESNWNIMQYLPQTASCQKYFLMIISAYIAAVYHSMRQELRRNAPGATPIKRRGATQASQQPSGDVSALPGLITFSQEYVSSELTQNFFTITQKIQKKVSSTRSVTLPSEMFPVLPGSHLPLNNPALEFIKYVCQVLSLDANIVNQVNKLKRDLLRLVDVGEFSEDAQFRDPCKSYVLPEVICHHCNFCRDLDLCKDPAVSQDGSVLPQWFCSNCQAQYDTESIEMALVEALQKKLMSYTLQDVECAKCKGVKEANMPLYCSCAGDFNLTFTTKSFSEQVEVFRNIAAHYNMSFLLETIDWIISMNT

Target ORF information:

RefSeq Version	NM_001128523.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed), epsilon (pole), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128523.1

ORF Insert Sequence:

ATGGTTTTAC AAAACAGTGG ACGATATAAA GCTGACCGAG GCGGAGATCA AGACAACCAG 
CAGGATGATG CGTCAGCTTT GTCTGCTGTC AAGCGCCTGG AGCGCAGTCA GTTCACTGAC 
AGTATGGACG AGCGTTTCGG CTTTGAACGG ATGAAGGAGC CTGGGGAGAA AACAGGATGG 
CTTATCAACA TGCACCCGAC TGAGATTCTG GATGATGACA AACGAATGAT CAGTGCTGTG 
GACTATTACT TCATACAGGA AGATGGAAAC AGATTCAAGG TTGCTCTTCC CTACAAGCCA 
TATTTCTATA TTGCCACTAA AAAGAACACT GAAAGAGAAG TGATCTCCTT CCTGTCAAAG 
AAGTTCCAAG GGAAAGTAGC GAAGCTTGAA TCTGTGCCAA AGGAAGATTT AGATCTGCCT 
AACCACCTGG TCGGACTAAA GAGGAACTAC ATCAAACTCT CTTTTAACAC TGTGGATGAT 
CTTATTAAAG TTAAAAGAGA GATCTCACCT GCTGTCCGCA AGAACAGGGA GAGAGAGAAG 
TCTAATGATG CCTATACAAG CATGCTGTCC AGCGCTTTGG TTGGTGGGAG TGTAGTGACG 
GAGGATGAGG GTGGATCGTC AAAAAAGATG ACCGAGCAGC TAGACAACAT CCTAGACATG 
CGGGAATATG ATGTGCCCTA TCACGTCCGC GTTTCTATTG ACTTAAAGAT TCACGTGGCT 
CACTGGTATA ATGTGCGGTA TAGAGGAAGT GCTTATCCTC CTGAGATTGT GCGCAGGGAC 
GACCTTGTGG AAAGACCTGA TCCTGTTGTG TTGGCTTTTG ATATTGAGAC CACTAAACTT 
CCTCTGAAGT TTCCTGATGC TGAAACAGAC CAGATCATGA TGATATCATA CATGATAGAT 
GGACAGGGTT TCCTCATCAC AAACAGAGAA ATTGTGTCTG AGGACATTGA AGACTTTGAG 
TTCACCCCAA AACCTGAATA TGAGGGCCCT TTCACTATAT TTAATGAGCC TGATGAGGCC 
TCTCTCATTC AGAGATGGTT TGAGCACGTC CATGAGACCA AGCCAAATAT TTTTGTCACC 
TACAACGGGG ATTTCTTTGA CTGGCCATTT GTAGAAGCCA GAGCTGCCCA GTTAGGTCTG 
AGTATGCATC GGGAGATCGG CTTCCAGAAG GACAATCAGG GAGAATATAA AGCCAGCCAG 
GCTATTCACA TGGACTGCCT CAGGTGGGTA AAGAGAGACA GTTACCTGCC TGTAGGAAGT 
CATAACCTCA AGGCTGCAGC AAAAGCTAAA TTAGGCTATG ACCCTGTAGA GCTGGACCCA 
GAGGAGATGT GCCGCATGGC CACTGAAGAA CCACAGACCC TGGCCACCTA TTCAGTGTCT 
GACGCTGTGG CTACATACTA CCTCTACATG AAATATGTTC ATCCCTTCAT TTTTGCCCTC 
TGCACTATTA TCCCTATGGA ACCTGATGAG GTTTTGCGGA AAGGCTCCGG TACCTTGTGC 
GAAGCCTTGT TGATGGTTCA GGCCTACCAT GTCAACATCG TCTTCCCAAA CAAGCAGGAG 
CAGGTCTTTA ACAAACTCAC AGATGATGGG CATGTATTGG ACTCTGAGAC ATATGTGGGC 
GGCCACGTCG AGGCTCTGGA GTCTGGTGTG TTTCGCAGTG ACATCCCCTG CCGCTTTAAA 
ATGAATCCTG CAGCGTTTGA CTTTTTGATT CAGAGGGTGG AACGTACTCT TCGTCATGCA 
ATTGAGGAAG AGGAGAAGAT TCCTCTAGAG CAGGTTACCA ACTTTAATGA GGTGTGTGAT 
GAGATCAAGA GGAAGCTGAT CTCTTTGAAG GAAGTGCCCA ACAGAATCGA GTGTCCTCTT 
ATTTACCATC TTGATGTAGG GGCCATGTAC CCCAACATCA TCCTCACCAA CAGACTACAG 
CCTTCTGCCA TGGTTGATGA GGCGACCTGT GCCGCCTGTG ATTTTAATAA ACCTGGGGCA 
AACTGTCAGC GCAGAATGAC TTGGCAATGG AGAGGAGAGA TCATGCCAGC AAGTAGGAGT 
GAGTTTCATC GCATTCAGCA GCAACTGGAG TCCGAGAAGT TTCCCCCTGT TTTTCCTAAT 
GGTCCACCTC GTGCTTTCCA TGAGCTCAAT AGAGAAGAAC AGGCTCGGCA TGAGAAGAAG 
CGTTTAGGAG ACTACTGTAG AAAAGCCTAT AAGAAGGTTC ACCTGACCAG GCTGGAGGAG 
AGAGTCACTA CTATCTGCCA GAGAGAAAAC TCCTTTTATG TTGACACAGT TCGAGCCTTC 
AGAGACCGTC GATATGAGTT TAAAGGACTC CACAAGGTGT GGAAGAAAAA GCTGTCTACA 
GCACAGGATA ATGGGGACGC AGCGGAGGTG AAGCGCTGCA AGAACATGGA GATTCTTTAT 
GAGTCTCTTC AGCTGGCCCA CAAGTGCATC CTCAACTCCT TTTACGGATA CGTCATGAGA 
AAAGGAGCAC GTTGGTACTC AATGGAGATG GCTGGGATTG TTTGTTACAC CGGTGCCAAC 
ATCATCACAC AGGCCAGAGA ACTCATCGAG CAGATTGGGA GGCCTCTGGA GTTGGACACT 
GATGGTATAT GGTGTGTTCT TCCCAATACC TTTCCTGAGA ATTTTGTCAT CAAATCCACC 
AATGAGAAGA AGCCAAAAGT GACCATTAGT TATCCTGGAG CCATGCTCAA TATTATGGTC 
AAGGAGGGCT TCACAAATCA TCAGTATCAA GAGTTGGTGG ACGCCGCATC TCTTACCTAT 
GAGACCAGAG CTGAGAACAG TATCTTCTTT GAAGTTGATG GCCCGTACTT GGCTATGATC 
CTGCCTGCCT CCAAAGAAGA GGGAAAGAAA CTAAAGAAAA GATATGCTGT GTTCAATGAG 
GACGGCTCTC TGGCAGAGCT GAAGGGGTTT GAAGTGAAGA GGAGAGGAGA ACTTCAGCTC 
ATTAAGATCT TCCAGTCGTC TGTGTTCGAG GCGTTCTTGA AAGGCACAAC TCTGGAAGAG 
GTTTACGCCT CTGTGGCTAA AGTGGCTGAC TACTGGCTTG ATGTGCTCTA CAGCAAGGCT 
GCTAACATGC CAGATGCAGA GTTGTTTGAG CTGATTTCGG AAAACCGTTC GATGTCTCGT 
AAACTGGAAG ATTATGGTGA ACAGAAATCC ACCTCGATTA GCACCGCCAA ACGTCTTGCT 
GAGTTTCTTG GCGATCAGAT GGTGAAGGAC GCCGGCCTCA GCTGTCGATA TGTCATCTCC 
CGCAAACCAG AGGGCTCGCC TGTCACTGAG AGGGCAATCC CGCTAGCCAT TTTTCAGGCT 
GAAGAGAGTG TTAAAAAGCA TTTTCTACGG AAATGGCTGA AGATGCCCAG TCTCCATGAC 
CTTGACATTA GATCTATTCT GGACTGGAGT TACTACATTG AGCGTTTGGG CAGTGCTATT 
CAGAAAATCA TCACAATCCC CGCGGCTCTA CAACAAGTTA AAAACCCAGT GCCCAGAGTA 
CGCCATCCAG ACTGGCTGCA CAAGAAACTT CTGGAAAAGA ATGACATCTA CAAGCAGAAG 
AAGATTAGCG AGCTCTTCAC CAGTGAGGGA AAGAGACAGG TGGCCCAACA AACTCTAGCA 
GCAGGCGAGA CTCCAGATAT GGAGGATTTG GCTGCTCCTC AGCGCCCCGT GCAGCCTGCT 
ATCCTCATCA GCACAAAGAG GAAGAGGATC TCTCAAGGAG AGGACAGTCA GACTGAGACT 
CAAGAGCAAG AACTCACACA GTCTTGGAGA GAAATACTGG GCCCACCACC GGCCATGGGT 
GCCACCCGGG AGGAAATATT AGTCTGGTTG CGCTACCATA AGAAAAAATG GGAGCTGCAG 
CTTCGGCAGA GAAAGGAACG TCGGAAGCGG CGGCGTTTGA TTGATGGAGA ATCTCAGCCG 
ACTGGAGGCG GTGTTATCCG TGGGGGACCC ACAACAGGGC TTGGCAGCTT CCTGCGGAGG 
ACCGCTCGCA GTATTCTAGA CATGCCTTGG CAGATTGTAC AGATTGCAGA GACCAGTCAC 
CCAGGCCTTT ATAAGCTGTG GGCCGTCATT GGCAGTGACC TCCACTGCAT GAAACTCAAC 
ATCCCTCGAG TGTTTTATGT CAACCAGAGA GTGCCTAAAC AAGAAGAGGC TGCTACCTGC 
AAAAAGGTGA ACCGGATCCT TCCTCGCTCT GGTGTGGCGT GTTTCCTGTA CCAGTATACT 
GTTCCTGAGG ACATGTACCA GGAACACATC AATGAAATCA ATGCTGACCT CTCGGCTCCT 
GATATTGAGG GCGTCTATGA GACACAGGTT CCTTTACTGT TCCGGGCTTT GGTCCAGCTG 
GGCTGCATGT GTATGGTCAA TAAACATGTG GTCAGAGACC TGGCTGGCAG AGAGGCGGAT 
ACCTTTGACC TGGAGCACCT AGAGATGAGA TCCTTGGCCC AGTTTAGCTA CCTTGAGCCA 
GGCAGTGTAA GGCACATCTA CCTGTACCAT CACAGTCAGG GTCATAAGGC TCTCTTTGGC 
CTCTTCATAC CATCGCAACG CAAAGCCAGC ATCTTTGTTC TGGACACGGT CCGCAGTAAT 
CAGATGCCCA ACCTGAACTC ACTGTATGGA GCCGAGCGCA CAGCACTGCT GGAGAAAACC 
ACAGAGGAGC TTCTGCCACC CGAGAAACAT CTGTTTGAAG TGCGTGCTGA AAATGACATC 
AAAGCCATCT ACCGTGCACT GCAGCGCATC CTCCTCAACT ACAAGGAGGA GCGCCGGGGT 
CCAACCCTGA TTGCTGTTCA GTCCAACTGG GAGCTGCGGC GTCTGGCGGC CGGTATGACT 
GTACTGGAGG AGTTCCCGGT GGTGCCAGTT CACGTTATAG ATGAGATCAG CTATAATGTA 
CTGGACTGGC AAAGGCACGG AGCTCGACGC ATGATTAAAC ACTACCTGAA CCTGGACAGC 
TGCCTCTCAC AGGCTTTTGA TATGGCCAGG TATTATCACC TGCCTGTAGG AAACCTGCCT 
CAGGACATTT CTATATTTGG ATCGGATCTA TTTCTTGCAC GTCATCTGAG AAAGCACAAC 
CACCTGTTGT GGCTCTCACC CACGGCACGG CCGGACCTCG GGGGAAAAGA GGCTGACGAC 
AGCAGGCTGG TGATGGAGAG TGATGAGAGA GGTTCCATGG AAATTAATAG CCAGGGCTGC 
TACTCCACAG TTTGTGTGGA GCTGGACATT CAGAGTTTGG CGGTAAACAC CATTCTGCAG 
TCTCAGCATG TAAACGACAT GGAGGGCGGA GCCAGCATTG GGGTCAGCTT TGATGTCATT 
CAGCACGCAT CGCTGGAGGA GATGATGTCA GGGAATCAGG GGGCGAATGC GGTGGCCAGC 
TATGATGAGA CTGCACTCTG CTCCAACACA TTCAGGATTC TGAAGAGTAT GGTTGTTGGT 
TGGGTTCGAG AGATCACACA GTATCATAAC GTCTATGCTG ATAACCAGGT GATGCATTTT 
TATCGGTGGT TGCGCTCACC CAGTTCTCTA CTATATGACC CGGCTCTGCA CCGCACGCTG 
CTTAACATGA TGAAGAAGAT CTTTCTACAA CTCGTGGCAG AGTTTAAACG CTTGGGATCA 
TCTGTGATCT ATGGAAACTT CAACCGTCTC ATCCTCTGCA CCAAGAAACG ACGCATTGAT 
GACGCCATTG CATATGTGGA GTACATCACT AACAGTATCC ACACGAAGGA GATCTTCCAC 
TCTCTGTCCA TCACATTTTC CCGCTGTTGG GAGTTTCTGC TGTGGATGGA TCCTGCTAAC 
TGTGGAGGAG TGAAGGGAAA GCTGCCCTCC AGCCTTCTGT ATGGAGAGCA TGACAGTGAA 
AAGAAGAAGA AAGATGGAGC AGAAGAGGAT GGAAGTGATG ATGAAGATGA ACATGAAGAA 
AACCAAGAGG AAGATGAAGA ACAGGACAGG GAGGAAGACG TGGAGGATCT GATTGAAAGT 
AACTGGAACA TAATGCAGTA TCTTCCTCAG ACGGCCTCCT GTCAGAAATA CTTTCTCATG 
ATCATTTCAG CCTACATCGC AGCTGTTTAT CACAGCATGA GACAAGAACT TCGGCGAAAC 
GCCCCTGGAG CCACTCCCAT TAAGAGACGA GGAGCAACAC AGGCATCCCA GCAGCCATCT 
GGAGATGTCA GTGCGCTTCC TGGTTTGATT ACATTCTCCC AGGAGTATGT GTCAAGTGAA 
CTCACACAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTGTC GAGCACTCGC 
AGTGTTACGC TCCCGTCTGA GATGTTCCCT GTTCTTCCTG GTTCTCATTT GCCGCTCAAC 
AACCCTGCGC TGGAGTTCAT CAAATACGTC TGCCAGGTTC TCTCTCTGGA CGCCAACATT 
GTCAATCAGG TGAACAAACT AAAGCGAGAT CTTCTGCGCT TGGTGGATGT TGGAGAGTTT 
TCAGAAGACG CCCAGTTTCG TGACCCCTGC AAGTCATATG TCCTGCCAGA GGTCATCTGT 
CACCACTGCA ACTTCTGTCG AGACCTGGAC CTCTGTAAAG ACCCTGCAGT TTCACAGGAT 
GGTTCAGTTC TTCCACAATG GTTCTGTTCT AACTGTCAGG CTCAGTACGA CACTGAGTCT 
ATAGAAATGG CTCTGGTGGA GGCCCTGCAG AAGAAACTAA TGAGCTACAC GCTGCAAGAT 
GTGGAGTGTG CCAAATGCAA AGGAGTGAAG GAGGCGAACA TGCCGCTGTA CTGTAGCTGT 
GCTGGAGATT TCAACCTCAC CTTCACCACA AAGAGCTTCT CTGAGCAGGT GGAAGTTTTC 
AGAAACATCG CTGCTCATTA TAATATGAGC TTCTTATTGG AGACCATTGA CTGGATCATC 
AGTATGAACA CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Forward Genetic Screens in Zebrafish Identify Pre-mRNA-Processing Pathways Regulating Early T Cell Development.
Cell reports17(9)2259-2270(2016 11)
Iwanami N,Sikora K,Richter AS,Mönnich M,Guerri L,Soza-Ried C,Lawir DF,Mateos F,Hess I,O'Meara CP,Schorpp M,Boehm T

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pole cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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