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a Related Biological Terms:

flippase

A putative enzyme that transports membrane elements, e.g. the lipopolysaccharide of Gram-negative bacteria, from the inner to the outer leaflet of the lipid bilayer cell membrane. Raetz, C.H.R. (1990) Annu. Rev. Biochem. 59, 129-170

flow FAB

Flow fast-atom bombardment. (see fast-atom bombardment mass spectrometry (FAB-MS))

fluctuating embryo

(see framework model)

fluid mosaic model (Singer-Nicolson model)

A conceptualization of a plasma membrane as a lipid bilayer in which embedded proteins are mobile in two dimensions. (see also Danielli-Davson model; Gortner and Grendel model; unit membrane Danielli-Davson model)Singer, S.J. and Nicholson, G.L. (1972) Science 175, 723-731 Learn more about restriction enzymes.

fluorescence correlation spectroscopy

The observation of fluctuations in the concentration of sparse fluorophores in a small observation area. Applications include estimation of transport coefficients as a fluorophore repopulates a bleached area, and of very low concentratons as individual molecules diffuse in and out of the experimental field of vision.Maiti, S., Haupts, U. and Webb, W.W. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 11753-11757

fluorescence in situ hybridization (FISH)

A method for visualization of a genetic marker on a chromosome by use of a fluorescently labelled polynucleotide probe that hybridizes to and indicates the locus of a gene on a chromosome during mitosis (metaphase). (see also interphase nucleus mapping) Related tool: real time pcr

fluorescence photobleaching recovery

A technique to measure the mobility of membrane lipids by destruction of fluorophore-tagged molecules in a very small area of a membrane and measurement of the rate at which new molecules migrate into it from the non-affected margins.

fluorescence recovery after photobleaching

(= fluorescence photobleaching recovery)

fluorescence resonance energy transfer

(= resonance energy transfer)

fluorescence-activated cell sorting (FACS)

A technique for separation of cells according to their fluorescence after attachment of a fluorophore to specific cells, e.g. with a fluorescent antibody. Droplets that contain no more than one cell are passed by a device that imposes an electrical charge on fluorescent droplets and deflects them into their own receptacle. (see also cytoflow; flow cytometry) Related tool: real time pcr

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