rapgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rapgef1
Entrez Gene ID	733953
Full Name	Rap guanine nucleotide exchange factor 1
General protein information	Preferred Names Rap guanine nucleotide exchange factor 1 Names rap guanine nucleotide exchange factor 1 Rap guanine nucleotide exchange factor (GEF) 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045665.1	NP_001039130.1	rap guanine nucleotide exchange factor 1
XM_031890514.1	XP_031746374.1	rap guanine nucleotide exchange factor 1 isoform X3
XM_012968553.3	XP_012824007.1	rap guanine nucleotide exchange factor 1 isoform X4
XM_012968552.2	XP_012824006.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_012968552.3	XP_012824006.1	rap guanine nucleotide exchange factor 1 isoform X2
XM_012968551.2	XP_012824005.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_012968551.3	XP_012824005.1	rap guanine nucleotide exchange factor 1 isoform X1
XM_012968553.2	XP_012824007.1	rap guanine nucleotide exchange factor 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF1	XP_001167804.1
Mus musculus (house mouse)	Rapgef1	NP_001034175.1
Homo sapiens (human)	RAPGEF1	NP_941372.1
Canis lupus familiaris (dog)	RAPGEF1	XP_005625272.1
Bos taurus (cattle)	RAPGEF1	NP_001192801.1
Rattus norvegicus (Norway rat)	Rapgef1	XP_006233952.1
Gallus gallus (chicken)	RAPGEF1	XP_004945960.1
Xenopus tropicalis (tropical clawed frog)	rapgef1	NP_001039130.1
Macaca mulatta (Rhesus monkey)	RAPGEF1	XP_001105413.1
Danio rerio (zebrafish)	rapgef1a	XP_005166961.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rapgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rapgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22617	NM_001045665.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28737	XM_012968551.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28738	XM_012968552.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28739	XM_012968553.2 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63734	XM_012968551.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63735	XM_012968552.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63736	XM_031890514.1 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63737	XM_012968553.3 Latest version!	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rapgef1 ( NM_001045665.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001039130.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), mRNA.

Price & Availability↑

CloneID	OXa22617
Clone ID Related Accession (Same CDS sequence)	NM_001045665.1
Accession Version	NM_001045665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR942489.2. ##Evidence-Data-START## Transcript exon combination :: CR942489.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCGGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACC CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC TAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCCGAC GGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGGGG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATATACC AGCAGAAGAA CAAGCATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCGTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGTT 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGGATT CTCCTTTACC CAATGAAGGG 
CCTCAGCAGA ACTGCACCCT GCCCTGTCTG CCAGGCAAAG ACAACCACGA GGCATCAGAG 
AGGCCTCCAA AGTCCCCCGA TTGCCACAGC GATGAGAATG GCGAGGGGGA AGAGGAAGAA 
GAGCTCGATG AACTCTCCCT TGTTGACCAT AACGAGATTA TGGCTAAGCT GACCCTGAAG 
CAAGAGGGTG ATGATGGACC GGACGTGCGG GGCGGCTCCG GTGACATCTT GCTGGTCCAT 
GCAACGGAAA CCGATCGCAA AGATCTCGTC CTGTACTTCG AAGCCTTCCT GACAACATAC 
CGCACCTTTA TTACCCCCGA GGAGCTGATT CAGAAGCTCC AGTACAGATA TGAGAGGTTC 
TGTCACTTTC AAGACACTTT CAAGCAGCGC GTGAGCAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ACGAGTTATG CTTGGTCGAG ATGACAGATG AGATCCTGAA GCTGCTGATG 
GAACTCGTCT TCCGTCTGGT GTGTAAAGGG GAACTTAGCC TGGCTCGCAT CTTGCGCAAG 
AACATTCTGG AAAAGGTGGA GAATAAGAGA ATGCTGCACC ACGCCAATTC TGCACTTAAG 
CCCCTGGCTG CCAGAGGAGT AGCAGCAAGG CCAGGGACGC TCCACGATTT CCACAGTCTT 
GAAATTGCAG AGCAGCTGAC TCTGCTGGAT GCAGAACTCT TCTATAAAAT AGAGATTCCT 
GAGGTTCTGC TTTGGGCAAA AGAGCAAAAC GAGGAGAAGA GCCCCAATTT AACACAGTTC 
ACAGAGCACT TTAACAACAT GTCATACTGG GTCCGATCCA TTATCATGCT TCAGGAGAAA 
GCCCAGGACC GAGAGCGATT GCTGCTTAAA TTCATTAAAA TCATGAAGCA CCTTCGGAAA 
CTGAACAACT TTAACTCCTA CCTTGCCATC CTGTCTGCGC TGGACTCCGC CCCCATTCGC 
AGGCTGGAAT GGCAGAAGCA GACGTCAGAG GGCCTGGCGG AATACTGCAC ATTGATTGAC 
AGCTCGTCCT CCTTCAGAGC CTATAGGGCA GCCCTGGCTG AGGTTGAACC CCCCTGTATC 
CCCTACTTAG GCCTGATCCT GCAGGATCTT ACCTTTGTAC ATCTGGGGAA CCCCGACCAC 
ATTGATGGGA AAGTCAACTT CTCCAAGCGT TGGCAGCAGT TCAACATTTT GGACAGTATG 
AGGCGATTCC AGCAAGTACA TTACGAGATC CGCCGCAACG ATGAAATCAT CTCCTTCTTC 
AACGACTTCA GCGACCACCT GGCCGAGGAG GCTCTGTGGG AACTGTCCCT AAAGATAAAA 
CCTCGCAACG TCACCCGGCG CAAAACAGAA AGGGACGAGA AAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039130.1
CDS	26..3133
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDGIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLGDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERVPPPALPPKQRQMDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNVTRRKTERDEKT

Target ORF information:

RefSeq Version	NM_001045665.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045665.1

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCGGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACC CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC TAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCCGAC GGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGGGG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATATACC AGCAGAAGAA CAAGCATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCGTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGTT 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGGATT CTCCTTTACC CAATGAAGGG 
CCTCAGCAGA ACTGCACCCT GCCCTGTCTG CCAGGCAAAG ACAACCACGA GGCATCAGAG 
AGGCCTCCAA AGTCCCCCGA TTGCCACAGC GATGAGAATG GCGAGGGGGA AGAGGAAGAA 
GAGCTCGATG AACTCTCCCT TGTTGACCAT AACGAGATTA TGGCTAAGCT GACCCTGAAG 
CAAGAGGGTG ATGATGGACC GGACGTGCGG GGCGGCTCCG GTGACATCTT GCTGGTCCAT 
GCAACGGAAA CCGATCGCAA AGATCTCGTC CTGTACTTCG AAGCCTTCCT GACAACATAC 
CGCACCTTTA TTACCCCCGA GGAGCTGATT CAGAAGCTCC AGTACAGATA TGAGAGGTTC 
TGTCACTTTC AAGACACTTT CAAGCAGCGC GTGAGCAAGA ACACCTTCTT CGTGCTGGTG 
AGAGTGGTGG ACGAGTTATG CTTGGTCGAG ATGACAGATG AGATCCTGAA GCTGCTGATG 
GAACTCGTCT TCCGTCTGGT GTGTAAAGGG GAACTTAGCC TGGCTCGCAT CTTGCGCAAG 
AACATTCTGG AAAAGGTGGA GAATAAGAGA ATGCTGCACC ACGCCAATTC TGCACTTAAG 
CCCCTGGCTG CCAGAGGAGT AGCAGCAAGG CCAGGGACGC TCCACGATTT CCACAGTCTT 
GAAATTGCAG AGCAGCTGAC TCTGCTGGAT GCAGAACTCT TCTATAAAAT AGAGATTCCT 
GAGGTTCTGC TTTGGGCAAA AGAGCAAAAC GAGGAGAAGA GCCCCAATTT AACACAGTTC 
ACAGAGCACT TTAACAACAT GTCATACTGG GTCCGATCCA TTATCATGCT TCAGGAGAAA 
GCCCAGGACC GAGAGCGATT GCTGCTTAAA TTCATTAAAA TCATGAAGCA CCTTCGGAAA 
CTGAACAACT TTAACTCCTA CCTTGCCATC CTGTCTGCGC TGGACTCCGC CCCCATTCGC 
AGGCTGGAAT GGCAGAAGCA GACGTCAGAG GGCCTGGCGG AATACTGCAC ATTGATTGAC 
AGCTCGTCCT CCTTCAGAGC CTATAGGGCA GCCCTGGCTG AGGTTGAACC CCCCTGTATC 
CCCTACTTAG GCCTGATCCT GCAGGATCTT ACCTTTGTAC ATCTGGGGAA CCCCGACCAC 
ATTGATGGGA AAGTCAACTT CTCCAAGCGT TGGCAGCAGT TCAACATTTT GGACAGTATG 
AGGCGATTCC AGCAAGTACA TTACGAGATC CGCCGCAACG ATGAAATCAT CTCCTTCTTC 
AACGACTTCA GCGACCACCT GGCCGAGGAG GCTCTGTGGG AACTGTCCCT AAAGATAAAA 
CCTCGCAACG TCACCCGGCG CAAAACAGAA AGGGACGAGA AAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968551.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824005.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28737
Clone ID Related Accession (Same CDS sequence)	XM_012968551.2
Accession Version	XM_012968551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC 
CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA 
CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC 
GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG 
GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG 
CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC 
TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC 
CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA 
TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC 
TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG 
AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC 
ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT 
TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT 
TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA 
ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT 
TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG 
CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG 
CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC 
GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC 
ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA 
CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG 
AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT 
TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC 
ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC 
CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824005.1
CDS	306..3665
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMQGSFAASSFSSVSYCAQTTKGVFPPEDTSHPQGISLSVSNCFLSRHGSFPVPSSENASEEVGEGEYVNLYSSGQSNGESFSFGGDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968551.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968551.2

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC 
CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA 
CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC 
GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG 
GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG 
CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC 
TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC 
CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA 
TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC 
TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG 
AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC 
ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT 
TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT 
TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA 
ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT 
TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG 
CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG 
CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC 
GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC 
ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA 
CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG 
AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT 
TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC 
ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC 
CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968552.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824006.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa28738
Clone ID Related Accession (Same CDS sequence)	XM_012968552.2
Accession Version	XM_012968552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG 
CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT 
TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT 
GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG 
GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA 
GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG 
GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC 
AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC 
TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG 
TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG 
AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA 
GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT 
CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA 
GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG 
TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG 
CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC 
CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG 
ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC 
TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG 
CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC 
TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT 
TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG 
GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC 
AAAACAGAAA GGGACGAGAA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824006.1
CDS	304..3570
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMQGSFAASSFSSVSYCAQTTKGVFPPEDTSHPQGISLSVSNCFLSRHGSFPVPSDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968552.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968552.2

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG 
CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT 
TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT 
GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG 
GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA 
GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG 
GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC 
AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC 
TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG 
TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG 
AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA 
GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT 
CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA 
GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG 
TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG 
CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC 
CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG 
ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC 
TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG 
CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC 
TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT 
TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG 
GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC 
AAAACAGAAA GGGACGAGAA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968553.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824007.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa28739
Clone ID Related Accession (Same CDS sequence)	XM_012968553.2
Accession Version	XM_012968553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG 
GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC 
TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT 
CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC 
AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC 
CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG 
CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC 
GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG 
ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG 
CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC 
GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA 
GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG 
AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA 
AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG 
GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA 
AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC 
TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT 
AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC 
ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA 
GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG 
GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT 
CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG 
CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG 
ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG 
GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA 
GAAAGGGACG AGAAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824007.1
CDS	303..3503
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMSENASEEVGEGEYVNLYSSGQSNGESFSFGGDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968553.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968553.2

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTAGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG 
GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC 
TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT 
CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC 
AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC 
CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG 
CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC 
GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG 
ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG 
CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC 
GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA 
GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG 
AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA 
AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG 
GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA 
AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC 
TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT 
AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC 
ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA 
GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG 
GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT 
CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG 
CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG 
ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG 
GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA 
GAAAGGGACG AGAAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968551.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824005.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

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CloneID	OXa63734
Clone ID Related Accession (Same CDS sequence)	XM_012968551.3
Accession Version	XM_012968551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968551.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC 
CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA 
CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC 
GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG 
GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG 
CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC 
TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC 
CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA 
TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC 
TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG 
AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC 
ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT 
TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT 
TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA 
ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT 
TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG 
CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG 
CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC 
GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC 
ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA 
CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG 
AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT 
TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC 
ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC 
CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824005.1
CDS	278..3637
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMQGSFAASSFSSVSYCAQTTKGVFPPEDTSHPQGISLSVSNCFLSRHGSFPVPSSENASEEVGEGEYVNLYSSGQSNGESFSFGGDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968551.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968551.3

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGAGTGA AAATGCCAGC GAGGAGGTGG GCGAGGGGGA ATACGTCAAC 
CTTTATTCCT CTGGGCAAAG CAACGGAGAG TCTTTTTCCT TTGGTGGTGA TTCTCCTTTA 
CCCAATGAAG GGCCTCAGCA GAACTGCACC CTGCCCTGTC TGCCAGGCAA AGACAACCAC 
GAGGCTTCAG AGAGGCCTCC AAAGTCCCCC GATTGCCACA GCGATGAGAA TGGCGAGGGG 
GAAGAGGAAG AAGAGCTCGA TGAACTCTCC CTTGTTGACC ATAACGAGAT TATGGCTAAG 
CTGACCCTGA AGCAAGAGGG TGATGATGGA CCGGACGTGC GGGGCGGCTC GGGTGACATC 
TTGCTGGTCC ATGCAACGGA AACCGATCGC AAAGATCTCG TCCTGTACTT CGAAGCCTTC 
CTGACAACAT ACCGCACCTT TATTACCCCC GAGGAGCTGA TTCAGAAGCT CCAGTACAGA 
TATGAGAGGT TCTGTCACTT TCAAGACACT TTCAAGCAGC GCGTGAGCAA GAACACCTTC 
TTTGTGCTGG TGAGAGTGGT GGACGAGTTA TGCTTGGTCG AGATGACAGA TGAGATCCTG 
AAGCTGCTGA TGGAACTCGT CTTCCGTCTG GTGTGTAAAG GGGAACTTAG CCTGGCTCGC 
ATCTTGCGCA AGAACATTCT GGAAAAGGTG GAGAATAAGA GAATGCTGCA CCACGCCAAT 
TCTGCACTTA AGCCCCTGGC TGCCAGAGGA GTAGCAGCAA GGCCAGGGAC GCTCCACGAT 
TTCCACAGTC TTGAAATTGC AGAGCAGCTG ACTCTGCTGG ATGCAGAACT CTTCTATAAA 
ATAGAGATTC CTGAGGTTCT GCTTTGGGCA AAAGAGCAAA ACGAGGAGAA GAGCCCCAAT 
TTAACACAGT TCACAGAGCA CTTTAACAAC ATGTCATACT GGGTCCGATC TATTATCATG 
CTTCAGGAGA AAGCCCAGGA CCGAGAGCGA TTGCTGCTTA AATTCATTAA AATCATGAAG 
CACCTTCGGA AACTGAACAA CTTTAACTCC TACCTTGCCA TCCTGTCCGC GCTGGACTCC 
GCCCCCATTC GCAGGCTAGA ATGGCAGAAG CAGACGTCAG AGGGCCTGGC GGAATACTGC 
ACATTGATTG ACAGCTCGTC CTCCTTCAGA GCCTATAGGG CAGCCCTGGC TGAAGTTGAA 
CCCCCCTGTA TCCCCTACTT AGGCCTGATC CTGCAGGATC TTACCTTTGT ACATCTGGGG 
AACCCCGACC ACATTGATGG GAAAGTCAAC TTCTCCAAGC GTTGGCAGCA GTTCAACATT 
TTGGACAGTA TGAGGCGATT CCAGCAAGTA CATTACGAGA TCCGCCGCAA CGACGAAATC 
ATCTCCTTCT TCAACGACTT CAGCGACCAC CTGGCCGAGG AGGCTCTGTG GGAACTGTCC 
CTAAAGATAA AACCTCGCAA CATCACCCGG CGCAAAACAG AAAGGGACGA GAAAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968552.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824006.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63735
Clone ID Related Accession (Same CDS sequence)	XM_012968552.3
Accession Version	XM_012968552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG 
CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT 
TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT 
GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG 
GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA 
GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG 
GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC 
AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC 
TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG 
TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG 
AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA 
GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT 
CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA 
GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG 
TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG 
CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC 
CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG 
ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC 
TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG 
CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC 
TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT 
TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG 
GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC 
AAAACAGAAA GGGACGAGAA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824006.1
CDS	278..3544
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMQGSFAASSFSSVSYCAQTTKGVFPPEDTSHPQGISLSVSNCFLSRHGSFPVPSDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968552.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968552.3

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGGATTC TCCTTTACCC AATGAAGGGC CTCAGCAGAA CTGCACCCTG 
CCCTGTCTGC CAGGCAAAGA CAACCACGAG GCTTCAGAGA GGCCTCCAAA GTCCCCCGAT 
TGCCACAGCG ATGAGAATGG CGAGGGGGAA GAGGAAGAAG AGCTCGATGA ACTCTCCCTT 
GTTGACCATA ACGAGATTAT GGCTAAGCTG ACCCTGAAGC AAGAGGGTGA TGATGGACCG 
GACGTGCGGG GCGGCTCGGG TGACATCTTG CTGGTCCATG CAACGGAAAC CGATCGCAAA 
GATCTCGTCC TGTACTTCGA AGCCTTCCTG ACAACATACC GCACCTTTAT TACCCCCGAG 
GAGCTGATTC AGAAGCTCCA GTACAGATAT GAGAGGTTCT GTCACTTTCA AGACACTTTC 
AAGCAGCGCG TGAGCAAGAA CACCTTCTTT GTGCTGGTGA GAGTGGTGGA CGAGTTATGC 
TTGGTCGAGA TGACAGATGA GATCCTGAAG CTGCTGATGG AACTCGTCTT CCGTCTGGTG 
TGTAAAGGGG AACTTAGCCT GGCTCGCATC TTGCGCAAGA ACATTCTGGA AAAGGTGGAG 
AATAAGAGAA TGCTGCACCA CGCCAATTCT GCACTTAAGC CCCTGGCTGC CAGAGGAGTA 
GCAGCAAGGC CAGGGACGCT CCACGATTTC CACAGTCTTG AAATTGCAGA GCAGCTGACT 
CTGCTGGATG CAGAACTCTT CTATAAAATA GAGATTCCTG AGGTTCTGCT TTGGGCAAAA 
GAGCAAAACG AGGAGAAGAG CCCCAATTTA ACACAGTTCA CAGAGCACTT TAACAACATG 
TCATACTGGG TCCGATCTAT TATCATGCTT CAGGAGAAAG CCCAGGACCG AGAGCGATTG 
CTGCTTAAAT TCATTAAAAT CATGAAGCAC CTTCGGAAAC TGAACAACTT TAACTCCTAC 
CTTGCCATCC TGTCCGCGCT GGACTCCGCC CCCATTCGCA GGCTAGAATG GCAGAAGCAG 
ACGTCAGAGG GCCTGGCGGA ATACTGCACA TTGATTGACA GCTCGTCCTC CTTCAGAGCC 
TATAGGGCAG CCCTGGCTGA AGTTGAACCC CCCTGTATCC CCTACTTAGG CCTGATCCTG 
CAGGATCTTA CCTTTGTACA TCTGGGGAAC CCCGACCACA TTGATGGGAA AGTCAACTTC 
TCCAAGCGTT GGCAGCAGTT CAACATTTTG GACAGTATGA GGCGATTCCA GCAAGTACAT 
TACGAGATCC GCCGCAACGA CGAAATCATC TCCTTCTTCA ACGACTTCAG CGACCACCTG 
GCCGAGGAGG CTCTGTGGGA ACTGTCCCTA AAGATAAAAC CTCGCAACAT CACCCGGCGC 
AAAACAGAAA GGGACGAGAA AACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_031890514.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746374.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa63736
Clone ID Related Accession (Same CDS sequence)	XM_031890514.1
Accession Version	XM_031890514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGTACAA ATCTGTTTTC CGCTCCTACT CCCAGGATTT TGCCCCTCAT 
AACCAAGCCC CACCACTTCT GGCCCCTGCC ATCTTCTACT CCTCCCGCTT CTCCCCCGTG 
CTCTCCGACT CCGCCTCTTC TCCCCCCGCC ATGCCCCAAG TACCCCTCCG CCCCCCCTTA 
CCTCCCTCTC AGGGTGGCAG CTCCTGCGGG ACCCCACCTA CCTGCCTTGC CTATGAGCCT 
TCTCTCTCCG GCACCTTGCA AACATCAGCG GATTCTCCTT TACCCAATGA AGGGCCTCAG 
CAGAACTGCA CCCTGCCCTG TCTGCCAGGC AAAGACAACC ACGAGGCTTC AGAGAGGCCT 
CCAAAGTCCC CCGATTGCCA CAGCGATGAG AATGGCGAGG GGGAAGAGGA AGAAGAGCTC 
GATGAACTCT CCCTTGTTGA CCATAACGAG ATTATGGCTA AGCTGACCCT GAAGCAAGAG 
GGTGATGATG GACCGGACGT GCGGGGCGGC TCGGGTGACA TCTTGCTGGT CCATGCAACG 
GAAACCGATC GCAAAGATCT CGTCCTGTAC TTCGAAGCCT TCCTGACAAC ATACCGCACC 
TTTATTACCC CCGAGGAGCT GATTCAGAAG CTCCAGTACA GATATGAGAG GTTCTGTCAC 
TTTCAAGACA CTTTCAAGCA GCGCGTGAGC AAGAACACCT TCTTTGTGCT GGTGAGAGTG 
GTGGACGAGT TATGCTTGGT CGAGATGACA GATGAGATCC TGAAGCTGCT GATGGAACTC 
GTCTTCCGTC TGGTGTGTAA AGGGGAACTT AGCCTGGCTC GCATCTTGCG CAAGAACATT 
CTGGAAAAGG TGGAGAATAA GAGAATGCTG CACCACGCCA ATTCTGCACT TAAGCCCCTG 
GCTGCCAGAG GAGTAGCAGC AAGGCCAGGG ACGCTCCACG ATTTCCACAG TCTTGAAATT 
GCAGAGCAGC TGACTCTGCT GGATGCAGAA CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
CTGCTTTGGG CAAAAGAGCA AAACGAGGAG AAGAGCCCCA ATTTAACACA GTTCACAGAG 
CACTTTAACA ACATGTCATA CTGGGTCCGA TCTATTATCA TGCTTCAGGA GAAAGCCCAG 
GACCGAGAGC GATTGCTGCT TAAATTCATT AAAATCATGA AGCACCTTCG GAAACTGAAC 
AACTTTAACT CCTACCTTGC CATCCTGTCC GCGCTGGACT CCGCCCCCAT TCGCAGGCTA 
GAATGGCAGA AGCAGACGTC AGAGGGCCTG GCGGAATACT GCACATTGAT TGACAGCTCG 
TCCTCCTTCA GAGCCTATAG GGCAGCCCTG GCTGAAGTTG AACCCCCCTG TATCCCCTAC 
TTAGGCCTGA TCCTGCAGGA TCTTACCTTT GTACATCTGG GGAACCCCGA CCACATTGAT 
GGGAAAGTCA ACTTCTCCAA GCGTTGGCAG CAGTTCAACA TTTTGGACAG TATGAGGCGA 
TTCCAGCAAG TACATTACGA GATCCGCCGC AACGACGAAA TCATCTCCTT CTTCAACGAC 
TTCAGCGACC ACCTGGCCGA GGAGGCTCTG TGGGAACTGT CCCTAAAGAT AAAACCTCGC 
AACATCACCC GGCGCAAAAC AGAAAGGGAC GAGAAAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746374.1
CDS	288..3809
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMQGSFAASSFSSVSYCAQTTKGVFPPEDTSHPQGISLSVSNCFLSRHGSFPVPSYKSVFRSYSQDFAPHNQAPPLLAPAIFYSSRFSPVLSDSASSPPAMPQVPLRPPLPPSQGGSSCGTPPTCLAYEPSLSGTLQTSADSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_031890514.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890514.1

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGCAGG GCTCCTTTGC TGCCTCTTCC 
TTCTCTTCTG TTTCGTACTG TGCACAGACA ACCAAAGGGG TCTTCCCACC CGAGGACACC 
AGCCACCCTC AGGGCATCAG TCTGTCCGTC TCTAACTGCT TTCTGAGCCG CCACGGCTCC 
TTTCCTGTGC CTTCGTACAA ATCTGTTTTC CGCTCCTACT CCCAGGATTT TGCCCCTCAT 
AACCAAGCCC CACCACTTCT GGCCCCTGCC ATCTTCTACT CCTCCCGCTT CTCCCCCGTG 
CTCTCCGACT CCGCCTCTTC TCCCCCCGCC ATGCCCCAAG TACCCCTCCG CCCCCCCTTA 
CCTCCCTCTC AGGGTGGCAG CTCCTGCGGG ACCCCACCTA CCTGCCTTGC CTATGAGCCT 
TCTCTCTCCG GCACCTTGCA AACATCAGCG GATTCTCCTT TACCCAATGA AGGGCCTCAG 
CAGAACTGCA CCCTGCCCTG TCTGCCAGGC AAAGACAACC ACGAGGCTTC AGAGAGGCCT 
CCAAAGTCCC CCGATTGCCA CAGCGATGAG AATGGCGAGG GGGAAGAGGA AGAAGAGCTC 
GATGAACTCT CCCTTGTTGA CCATAACGAG ATTATGGCTA AGCTGACCCT GAAGCAAGAG 
GGTGATGATG GACCGGACGT GCGGGGCGGC TCGGGTGACA TCTTGCTGGT CCATGCAACG 
GAAACCGATC GCAAAGATCT CGTCCTGTAC TTCGAAGCCT TCCTGACAAC ATACCGCACC 
TTTATTACCC CCGAGGAGCT GATTCAGAAG CTCCAGTACA GATATGAGAG GTTCTGTCAC 
TTTCAAGACA CTTTCAAGCA GCGCGTGAGC AAGAACACCT TCTTTGTGCT GGTGAGAGTG 
GTGGACGAGT TATGCTTGGT CGAGATGACA GATGAGATCC TGAAGCTGCT GATGGAACTC 
GTCTTCCGTC TGGTGTGTAA AGGGGAACTT AGCCTGGCTC GCATCTTGCG CAAGAACATT 
CTGGAAAAGG TGGAGAATAA GAGAATGCTG CACCACGCCA ATTCTGCACT TAAGCCCCTG 
GCTGCCAGAG GAGTAGCAGC AAGGCCAGGG ACGCTCCACG ATTTCCACAG TCTTGAAATT 
GCAGAGCAGC TGACTCTGCT GGATGCAGAA CTCTTCTATA AAATAGAGAT TCCTGAGGTT 
CTGCTTTGGG CAAAAGAGCA AAACGAGGAG AAGAGCCCCA ATTTAACACA GTTCACAGAG 
CACTTTAACA ACATGTCATA CTGGGTCCGA TCTATTATCA TGCTTCAGGA GAAAGCCCAG 
GACCGAGAGC GATTGCTGCT TAAATTCATT AAAATCATGA AGCACCTTCG GAAACTGAAC 
AACTTTAACT CCTACCTTGC CATCCTGTCC GCGCTGGACT CCGCCCCCAT TCGCAGGCTA 
GAATGGCAGA AGCAGACGTC AGAGGGCCTG GCGGAATACT GCACATTGAT TGACAGCTCG 
TCCTCCTTCA GAGCCTATAG GGCAGCCCTG GCTGAAGTTG AACCCCCCTG TATCCCCTAC 
TTAGGCCTGA TCCTGCAGGA TCTTACCTTT GTACATCTGG GGAACCCCGA CCACATTGAT 
GGGAAAGTCA ACTTCTCCAA GCGTTGGCAG CAGTTCAACA TTTTGGACAG TATGAGGCGA 
TTCCAGCAAG TACATTACGA GATCCGCCGC AACGACGAAA TCATCTCCTT CTTCAACGAC 
TTCAGCGACC ACCTGGCCGA GGAGGCTCTG TGGGAACTGT CCCTAAAGAT AAAACCTCGC 
AACATCACCC GGCGCAAAAC AGAAAGGGAC GAGAAAACTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rapgef1 ( XM_012968553.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824007.1 Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X4, mRNA.

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CloneID	OXa63737
Clone ID Related Accession (Same CDS sequence)	XM_012968553.3
Accession Version	XM_012968553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rap guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG 
GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC 
TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT 
CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC 
AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC 
CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG 
CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC 
GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG 
ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG 
CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC 
GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA 
GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG 
AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA 
AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG 
GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA 
AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC 
TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT 
AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC 
ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA 
GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG 
GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT 
CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG 
CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG 
ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG 
GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA 
GAAAGGGACG AGAAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824007.1
CDS	278..3478
Translation	MSGKIEKADSQRSHLSSFTMKLKDKFHSPKIKRTPSKKGKPADLTVKTEEKPVNKTLSRLEEQEKEVVNALRYFKTIVDKMAVDKMVLVMLPGSASKVLEAILPLVQVDTRCQESTAISSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKDVIKAVLDGVKELVRLMIEKQEHPSPTTTVKPSLPASKTDSTTELPLSERELEILNKTPEPTSKEAQVDSLESEELAPPKPPLPGIRVMDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPIGINNQDFEGDCYVRRRLSGGSQSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDNYDPDYEFLQEDLSRTDQVPGHGTILSPLPESLESASPFQGQSFQMPAGDTPNSEHSAVETPPALPQKKRRSTAPQTSDSIRGCYERHPSQYDNLCEDDLSPFVSAAMPPFAAVSPFQQGSPSQAEFLLDFVAAESDGIPENPPPLPEKKNKHMMTYMRFVEDYSEPQPSVFYQTPQNEHIYQQKNKHLREVYGFNDSFSSPESGHERAPPPALPPKQRQMSENASEEVGEGEYVNLYSSGQSNGESFSFGGDSPLPNEGPQQNCTLPCLPGKDNHEASERPPKSPDCHSDENGEGEEEEELDELSLVDHNEIMAKLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYFEAFLTTYRTFITPEELIQKLQYRYERFCHFQDTFKQRVSKNTFFVLVRVVDELCLVEMTDEILKLLMELVFRLVCKGELSLARILRKNILEKVENKRMLHHANSALKPLAARGVAARPGTLHDFHSLEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALAEVEPPCIPYLGLILQDLTFVHLGNPDHIDGKVNFSKRWQQFNILDSMRRFQQVHYEIRRNDEIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTERDEKT

Target ORF information:

RefSeq Version	XM_012968553.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rap guanine nucleotide exchange factor 1 (rapgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968553.3

ORF Insert Sequence:

ATGTCCGGGA AAATAGAGAA AGCAGACAGC CAGCGTTCCC ACCTCTCCTC TTTCACCATG 
AAGCTCAAGG ACAAATTCCA CTCCCCCAAA ATCAAGCGCA CCCCGTCCAA GAAGGGGAAG 
CCAGCCGACC TCACCGTCAA AACAGAAGAG AAACCCGTCA ACAAAACCTT AAGCCGCTTG 
GAGGAACAGG AGAAAGAAGT CGTTAATGCC TTGCGTTACT TTAAGACAAT TGTTGACAAG 
ATGGCGGTGG ACAAGATGGT GCTGGTGATG CTGCCAGGGT CGGCGAGCAA AGTGTTGGAA 
GCCATTTTGC CATTGGTGCA AGTGGATACT CGGTGTCAAG AAAGCACCGC CATCTCTTCC 
TGCTACAGCC GCGTGTACCA ATCCCTCGCC AATCTGATTC GCTGGTCTGA CCAGGTCATG 
CTGGAAGGCG TCAACTCGGA GGACAAGGAG ATGGTCACCA CCGTCAAAGA CGTGATAAAG 
GCCGTCCTGG ATGGCGTGAA GGAACTGGTG CGCCTGATGA TTGAGAAACA GGAACACCCA 
TCTCCCACTA CCACAGTGAA GCCCAGTTTA CCAGCATCCA AAACTGACAG CACCACAGAA 
TTGCCCTTAT CTGAGCGTGA GCTAGAAATC CTCAACAAGA CTCCTGAGCC GACATCTAAG 
GAAGCTCAAG TAGACAGTTT GGAGAGTGAA GAGTTAGCGC CGCCAAAGCC CCCGCTACCA 
GGAATCCGAG TAATGGATAA CAGCCCTCCA CCCGCTTTAC CCCCAAAGAA AAGACAATCT 
GCACCTTCAC CCACCCGAGT GGCAGTGGTG GCACCCATGA GCCGTGCCAC GAGTGGCTCC 
AGCTTGCCCA TTGGAATTAA TAACCAGGAT TTTGAAGGGG ATTGCTACGT ACGTCGCAGG 
TTATCCGGTG GAAGTCAGTC ATACGGGGGA GAGTCGCCGC GCCTTTCTCC CTGCAGTAGT 
ATAGGGAAGC TCAGCAAATC AGACGAGCAG CTCTCTTCGC TGGACAGAGA CAGCGGCCAG 
TGCTCCCGAA ACACCAGCTG TGAAACTCTA GATAACTATG ACCCAGACTA TGAATTCCTG 
CAAGAGGACC TCTCCAGAAC AGACCAAGTG CCGGGCCATG GAACCATTCT CAGCCCTCTC 
CCTGAATCCC TCGAGTCTGC CTCCCCCTTT CAAGGACAGA GCTTCCAGAT GCCCGCCGGC 
GACACCCCAA ATTCAGAGCA TTCGGCTGTA GAAACCCCAC CCGCCCTACC TCAGAAGAAA 
AGGAGGAGTA CGGCCCCCCA GACTTCTGAC AGTATCCGCG GCTGCTACGA AAGGCACCCC 
TCTCAGTATG ATAACCTCTG CGAAGATGAC TTGTCCCCAT TTGTTAGTGC CGCTATGCCT 
CCCTTTGCTG CCGTCTCGCC TTTCCAGCAA GGAAGCCCTT CTCAGGCAGA ATTCCTGTTG 
GACTTTGTTG CCGCAGAGTC GGATGGGATT CCAGAGAATC CTCCCCCACT CCCTGAGAAG 
AAGAACAAAC ATATGATGAC CTACATGCGC TTTGTAGAAG ACTACTCTGA ACCGCAGCCG 
TCCGTCTTCT ACCAAACCCC GCAGAACGAG CACATCTACC AGCAGAAGAA CAAACATCTC 
CGCGAGGTTT ACGGCTTCAA CGACTCCTTC AGCAGCCCCG AATCTGGCCA CGAGCGTGCC 
CCGCCCCCTG CCTTACCCCC AAAGCAAAGG CAAATGAGTG AAAATGCCAG CGAGGAGGTG 
GGCGAGGGGG AATACGTCAA CCTTTATTCC TCTGGGCAAA GCAACGGAGA GTCTTTTTCC 
TTTGGTGGTG ATTCTCCTTT ACCCAATGAA GGGCCTCAGC AGAACTGCAC CCTGCCCTGT 
CTGCCAGGCA AAGACAACCA CGAGGCTTCA GAGAGGCCTC CAAAGTCCCC CGATTGCCAC 
AGCGATGAGA ATGGCGAGGG GGAAGAGGAA GAAGAGCTCG ATGAACTCTC CCTTGTTGAC 
CATAACGAGA TTATGGCTAA GCTGACCCTG AAGCAAGAGG GTGATGATGG ACCGGACGTG 
CGGGGCGGCT CGGGTGACAT CTTGCTGGTC CATGCAACGG AAACCGATCG CAAAGATCTC 
GTCCTGTACT TCGAAGCCTT CCTGACAACA TACCGCACCT TTATTACCCC CGAGGAGCTG 
ATTCAGAAGC TCCAGTACAG ATATGAGAGG TTCTGTCACT TTCAAGACAC TTTCAAGCAG 
CGCGTGAGCA AGAACACCTT CTTTGTGCTG GTGAGAGTGG TGGACGAGTT ATGCTTGGTC 
GAGATGACAG ATGAGATCCT GAAGCTGCTG ATGGAACTCG TCTTCCGTCT GGTGTGTAAA 
GGGGAACTTA GCCTGGCTCG CATCTTGCGC AAGAACATTC TGGAAAAGGT GGAGAATAAG 
AGAATGCTGC ACCACGCCAA TTCTGCACTT AAGCCCCTGG CTGCCAGAGG AGTAGCAGCA 
AGGCCAGGGA CGCTCCACGA TTTCCACAGT CTTGAAATTG CAGAGCAGCT GACTCTGCTG 
GATGCAGAAC TCTTCTATAA AATAGAGATT CCTGAGGTTC TGCTTTGGGC AAAAGAGCAA 
AACGAGGAGA AGAGCCCCAA TTTAACACAG TTCACAGAGC ACTTTAACAA CATGTCATAC 
TGGGTCCGAT CTATTATCAT GCTTCAGGAG AAAGCCCAGG ACCGAGAGCG ATTGCTGCTT 
AAATTCATTA AAATCATGAA GCACCTTCGG AAACTGAACA ACTTTAACTC CTACCTTGCC 
ATCCTGTCCG CGCTGGACTC CGCCCCCATT CGCAGGCTAG AATGGCAGAA GCAGACGTCA 
GAGGGCCTGG CGGAATACTG CACATTGATT GACAGCTCGT CCTCCTTCAG AGCCTATAGG 
GCAGCCCTGG CTGAAGTTGA ACCCCCCTGT ATCCCCTACT TAGGCCTGAT CCTGCAGGAT 
CTTACCTTTG TACATCTGGG GAACCCCGAC CACATTGATG GGAAAGTCAA CTTCTCCAAG 
CGTTGGCAGC AGTTCAACAT TTTGGACAGT ATGAGGCGAT TCCAGCAAGT ACATTACGAG 
ATCCGCCGCA ACGACGAAAT CATCTCCTTC TTCAACGACT TCAGCGACCA CCTGGCCGAG 
GAGGCTCTGT GGGAACTGTC CCTAAAGATA AAACCTCGCA ACATCACCCG GCGCAAAACA 
GAAAGGGACG AGAAAACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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rapgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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