hmgcl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hmgcl
Entrez Gene ID	100494904
Full Name	3-hydroxymethyl-3-methylglutaryl-CoA lyase
General protein information	Preferred Names 3-hydroxymethyl-3-methylglutaryl-CoA lyase Names hydroxymethylglutaryl-CoA lyase, mitochondrial-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004915984.3	XP_004916041.1	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1
XM_002934559.5	XP_002934605.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
XM_031899112.1	XP_031754972.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
XM_002934559.4	XP_002934605.1	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1
XM_018092380.1	XP_017947869.1	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2
XM_031899113.1	XP_031754973.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X3
XM_031899111.1	XP_031754971.1	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	HMGCL	XP_001104722.2
Gallus gallus (chicken)	HMGCL	NP_001185643.1
Drosophila melanogaster (fruit fly)	CG10399	NP_609089.1
Canis lupus familiaris (dog)	HMGCL	XP_003638949.1
Pan troglodytes (chimpanzee)	HMGCL	XP_001166273.1
Homo sapiens (human)	HMGCL	NP_000182.2
Bos taurus (cattle)	HMGCL	NP_001068600.1
Mus musculus (house mouse)	Hmgcl	NP_032280.2
Rattus norvegicus (Norway rat)	Hmgcl	NP_077362.1
Arabidopsis thaliana (thale cress)	AT2G26800	NP_850087.1
Xenopus tropicalis (tropical clawed frog)	LOC100494904	XP_002934605.1
Danio rerio (zebrafish)	hmgcl	NP_957509.1
Caenorhabditis elegans (roundworm)	Y71G12B.10	NP_490889.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008717	XP_314824.4
Oryza sativa (rice)	Os01g0269000	NP_001042696.1

Gene Ontology

The following hmgcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hmgcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa12928	XM_004915984.3 Latest version!	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa12928	XM_002934559.4 Latest version!	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa43333	XM_018092380.1 Latest version!	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa54976	XM_031899112.1 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OXa54976	XM_031899111.1 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OXa54977	XM_002934559.5 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa54978	XM_031899113.1 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hmgcl ( XM_004915984.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916041.1 Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa12928
Clone ID Related Accession (Same CDS sequence)	XM_004915984.3 , XM_002934559.4
Accession Version	XM_004915984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915984.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916041.1
CDS	17..1012
Translation	MLAVSVRRAVALGWGAGWTPLAILRQLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_004915984.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915984.3

ORF Insert Sequence:

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_002934559.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934605.1 Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa12928
Clone ID Related Accession (Same CDS sequence)	XM_004915984.3 , XM_002934559.4
Accession Version	XM_002934559.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934559.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934605.1
CDS	17..1012
Translation	MLAVSVRRAVALGWGAGWTPLAILRQLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_002934559.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934559.4

ORF Insert Sequence:

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_018092380.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947869.1 Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa43333
Clone ID Related Accession (Same CDS sequence)	XM_018092380.1
Accession Version	XM_018092380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACACTTGTC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947869.1
CDS	17..922
Translation	MLAVSVRRAVALGWGAGWTPLAILRQLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTLVL

Target ORF information:

RefSeq Version	XM_018092380.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxymethyl-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092380.1

ORF Insert Sequence:

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC TGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACACTTGTC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_031899112.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754972.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54976
Clone ID Related Accession (Same CDS sequence)	XM_031899112.1 , XM_031899111.1
Accession Version	XM_031899112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTAT TAACGTTCAG AAGCACAGTT GGCAGCCTGC ACTTTTCCCT ACAGTCAGTT 
GCACTTAACC CTCTTTCATT AAAGCTCAGT TCCCTGCCAA TACAATCATT CCCTAAAGAG 
GTAAAGGTTG TGGAAGTTGG ACCCAGGGAT GGACTGCAAA ATGAAAAGAC AGTTGTCCCA 
TCTGAAGTGA AAATTCATCT CATTAACCTG TTATCTGAAG CAGGGCTTCA AGCTATTGAA 
GCCACCAGCT TTGTCTCTCC TAAATGGGTG CCTCAGATGG CCGATCATAA AAATGTCATT 
CAGGGAATAA AGAAGTACCC AAATGTAACC TACCCAGTCC TTACTCCAAA TCTTACTGGA 
TTTCAAGCTG CGGTTGAATG TGGTGCAAAG GAAGTAGCCA TATTTGGAGC TGCATCAGAG 
CTCTTTAGCA AGAAGAACAT TAACTGTTCT ATTGATGAAA GTTTGCAAAG ATTCAAGGAT 
GTTATTGCAG CAGCTAAGGA AGCAAACATC CCAGTGCGAG GGTATGTTTC ATGTGTTCTT 
GGATGCCCAT ATGAAGGCAA GGTAGCTCCT AGTAAAGTGG CTGAAGTGGC ATACAAGATG 
TTTTCTATGG GCTGCTATGA GATCTCTTTG GGTGACACGA TTGGAGTGGG AACTCCCGGC 
GGTATGAGGG ACATGCTGAG AGCTGTGTTG GATGTTGTTC CAGCCAAAGC TCTGGCTGTT 
CATTGCCATG ACACATATGG ACAGGCACTG GCAAATATAC TAGTTGCATT GCAGATGGGA 
GTGCAGGTTG TTGATGCTTC AGTTGCAGGT TTGGGAGGAT GCCCATATGC TCAGGGAGCT 
TCAGGGAATG TGGCAACTGA AGATGTTGTG TATATGCTGC ATGGTCTTGG AATTCAAACA 
GGTATTGACA TAAAGAAACT GACAGAAGCT GGAGCTTTTA TCTGCAAAGC ACTAGGAAAG 
AAGAGCAACT CAAAAGTGGC TCAAGCAATG TGCAAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754972.1
CDS	608..1609
Translation	MGLLTFRSTVGSLHFSLQSVALNPLSLKLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_031899112.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899112.1

ORF Insert Sequence:

ATGGGATTAT TAACGTTCAG AAGCACAGTT GGCAGCCTGC ACTTTTCCCT ACAGTCAGTT 
GCACTTAACC CTCTTTCATT AAAGCTCAGT TCCCTGCCAA TACAATCATT CCCTAAAGAG 
GTAAAGGTTG TGGAAGTTGG ACCCAGGGAT GGACTGCAAA ATGAAAAGAC AGTTGTCCCA 
TCTGAAGTGA AAATTCATCT CATTAACCTG TTATCTGAAG CAGGGCTTCA AGCTATTGAA 
GCCACCAGCT TTGTCTCTCC TAAATGGGTG CCTCAGATGG CCGATCATAA AAATGTCATT 
CAGGGAATAA AGAAGTACCC AAATGTAACC TACCCAGTCC TTACTCCAAA TCTTACTGGA 
TTTCAAGCTG CGGTTGAATG TGGTGCAAAG GAAGTAGCCA TATTTGGAGC TGCATCAGAG 
CTCTTTAGCA AGAAGAACAT TAACTGTTCT ATTGATGAAA GTTTGCAAAG ATTCAAGGAT 
GTTATTGCAG CAGCTAAGGA AGCAAACATC CCAGTGCGAG GGTATGTTTC ATGTGTTCTT 
GGATGCCCAT ATGAAGGCAA GGTAGCTCCT AGTAAAGTGG CTGAAGTGGC ATACAAGATG 
TTTTCTATGG GCTGCTATGA GATCTCTTTG GGTGACACGA TTGGAGTGGG AACTCCCGGC 
GGTATGAGGG ACATGCTGAG AGCTGTGTTG GATGTTGTTC CAGCCAAAGC TCTGGCTGTT 
CATTGCCATG ACACATATGG ACAGGCACTG GCAAATATAC TAGTTGCATT GCAGATGGGA 
GTGCAGGTTG TTGATGCTTC AGTTGCAGGT TTGGGAGGAT GCCCATATGC TCAGGGAGCT 
TCAGGGAATG TGGCAACTGA AGATGTTGTG TATATGCTGC ATGGTCTTGG AATTCAAACA 
GGTATTGACA TAAAGAAACT GACAGAAGCT GGAGCTTTTA TCTGCAAAGC ACTAGGAAAG 
AAGAGCAACT CAAAAGTGGC TCAAGCAATG TGCAAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_031899111.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754971.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X1, mRNA.

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CloneID	OXa54976
Clone ID Related Accession (Same CDS sequence)	XM_031899112.1 , XM_031899111.1
Accession Version	XM_031899111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATTAT TAACGTTCAG AAGCACAGTT GGCAGCCTGC ACTTTTCCCT ACAGTCAGTT 
GCACTTAACC CTCTTTCATT AAAGCTCAGT TCCCTGCCAA TACAATCATT CCCTAAAGAG 
GTAAAGGTTG TGGAAGTTGG ACCCAGGGAT GGACTGCAAA ATGAAAAGAC AGTTGTCCCA 
TCTGAAGTGA AAATTCATCT CATTAACCTG TTATCTGAAG CAGGGCTTCA AGCTATTGAA 
GCCACCAGCT TTGTCTCTCC TAAATGGGTG CCTCAGATGG CCGATCATAA AAATGTCATT 
CAGGGAATAA AGAAGTACCC AAATGTAACC TACCCAGTCC TTACTCCAAA TCTTACTGGA 
TTTCAAGCTG CGGTTGAATG TGGTGCAAAG GAAGTAGCCA TATTTGGAGC TGCATCAGAG 
CTCTTTAGCA AGAAGAACAT TAACTGTTCT ATTGATGAAA GTTTGCAAAG ATTCAAGGAT 
GTTATTGCAG CAGCTAAGGA AGCAAACATC CCAGTGCGAG GGTATGTTTC ATGTGTTCTT 
GGATGCCCAT ATGAAGGCAA GGTAGCTCCT AGTAAAGTGG CTGAAGTGGC ATACAAGATG 
TTTTCTATGG GCTGCTATGA GATCTCTTTG GGTGACACGA TTGGAGTGGG AACTCCCGGC 
GGTATGAGGG ACATGCTGAG AGCTGTGTTG GATGTTGTTC CAGCCAAAGC TCTGGCTGTT 
CATTGCCATG ACACATATGG ACAGGCACTG GCAAATATAC TAGTTGCATT GCAGATGGGA 
GTGCAGGTTG TTGATGCTTC AGTTGCAGGT TTGGGAGGAT GCCCATATGC TCAGGGAGCT 
TCAGGGAATG TGGCAACTGA AGATGTTGTG TATATGCTGC ATGGTCTTGG AATTCAAACA 
GGTATTGACA TAAAGAAACT GACAGAAGCT GGAGCTTTTA TCTGCAAAGC ACTAGGAAAG 
AAGAGCAACT CAAAAGTGGC TCAAGCAATG TGCAAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754971.1
CDS	608..1609
Translation	MGLLTFRSTVGSLHFSLQSVALNPLSLKLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_031899111.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899111.1

ORF Insert Sequence:

ATGGGATTAT TAACGTTCAG AAGCACAGTT GGCAGCCTGC ACTTTTCCCT ACAGTCAGTT 
GCACTTAACC CTCTTTCATT AAAGCTCAGT TCCCTGCCAA TACAATCATT CCCTAAAGAG 
GTAAAGGTTG TGGAAGTTGG ACCCAGGGAT GGACTGCAAA ATGAAAAGAC AGTTGTCCCA 
TCTGAAGTGA AAATTCATCT CATTAACCTG TTATCTGAAG CAGGGCTTCA AGCTATTGAA 
GCCACCAGCT TTGTCTCTCC TAAATGGGTG CCTCAGATGG CCGATCATAA AAATGTCATT 
CAGGGAATAA AGAAGTACCC AAATGTAACC TACCCAGTCC TTACTCCAAA TCTTACTGGA 
TTTCAAGCTG CGGTTGAATG TGGTGCAAAG GAAGTAGCCA TATTTGGAGC TGCATCAGAG 
CTCTTTAGCA AGAAGAACAT TAACTGTTCT ATTGATGAAA GTTTGCAAAG ATTCAAGGAT 
GTTATTGCAG CAGCTAAGGA AGCAAACATC CCAGTGCGAG GGTATGTTTC ATGTGTTCTT 
GGATGCCCAT ATGAAGGCAA GGTAGCTCCT AGTAAAGTGG CTGAAGTGGC ATACAAGATG 
TTTTCTATGG GCTGCTATGA GATCTCTTTG GGTGACACGA TTGGAGTGGG AACTCCCGGC 
GGTATGAGGG ACATGCTGAG AGCTGTGTTG GATGTTGTTC CAGCCAAAGC TCTGGCTGTT 
CATTGCCATG ACACATATGG ACAGGCACTG GCAAATATAC TAGTTGCATT GCAGATGGGA 
GTGCAGGTTG TTGATGCTTC AGTTGCAGGT TTGGGAGGAT GCCCATATGC TCAGGGAGCT 
TCAGGGAATG TGGCAACTGA AGATGTTGTG TATATGCTGC ATGGTCTTGG AATTCAAACA 
GGTATTGACA TAAAGAAACT GACAGAAGCT GGAGCTTTTA TCTGCAAAGC ACTAGGAAAG 
AAGAGCAACT CAAAAGTGGC TCAAGCAATG TGCAAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_002934559.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934605.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.

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CloneID	OXa54977
Clone ID Related Accession (Same CDS sequence)	XM_002934559.5
Accession Version	XM_002934559.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934559.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC CGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934605.1
CDS	111..1106
Translation	MLAVSVRRAVALGWGAGWTPLAILRQLSSLPIQSFPKEVKVVEVGPRDGLQNEKTVVPSEVKIHLINLLSEAGLQAIEATSFVSPKWVPQMADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_002934559.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934559.5

ORF Insert Sequence:

ATGTTAGCGG TGTCTGTTCG TAGAGCTGTA GCTTTGGGTT GGGGAGCGGG TTGGACACCT 
TTGGCGATCT TGCGGCAGCT CAGTTCCCTG CCAATACAAT CATTCCCTAA AGAGGTAAAG 
GTTGTGGAAG TTGGACCCAG GGATGGACTG CAAAATGAAA AGACAGTTGT CCCATCTGAA 
GTGAAAATTC ATCTCATTAA CCTGTTATCT GAAGCAGGGC TTCAAGCTAT TGAAGCCACC 
AGCTTTGTCT CTCCTAAATG GGTGCCTCAG ATGGCCGATC ATAAAAATGT CATTCAGGGA 
ATAAAGAAGT ACCCAAATGT AACCTACCCA GTCCTTACTC CAAATCTTAC TGGATTTCAA 
GCTGCGGTTG AATGTGGTGC AAAGGAAGTA GCCATATTTG GAGCTGCATC AGAGCTCTTT 
AGCAAGAAGA ACATTAACTG TTCTATTGAT GAAAGTTTGC AAAGATTCAA GGATGTTATT 
GCAGCAGCTA AGGAAGCAAA CATCCCAGTG CGAGGGTATG TTTCATGTGT TCTTGGATGC 
CCATATGAAG GCAAGGTAGC TCCTAGTAAA GTGGCTGAAG TGGCATACAA GATGTTTTCT 
ATGGGCTGCT ATGAGATCTC TTTGGGTGAC ACGATTGGAG TGGGAACTCC CGGCGGTATG 
AGGGACATGC TGAGAGCTGT GTTGGATGTT GTTCCAGCCA AAGCTCTGGC TGTTCATTGC 
CATGACACAT ATGGACAGGC ACTGGCAAAT ATACTAGTTG CATTGCAGAT GGGAGTGCAG 
GTTGTTGATG CTTCAGTTGC AGGTTTGGGA GGATGCCCAT ATGCTCAGGG AGCTTCAGGG 
AATGTGGCAA CTGAAGATGT TGTGTATATG CTGCATGGTC TTGGAATTCA AACAGGTATT 
GACATAAAGA AACTGACAGA AGCTGGAGCT TTTATCTGCA AAGCACTAGG AAAGAAGAGC 
AACTCAAAAG TGGCTCAAGC AATGTGCAAG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcl ( XM_031899113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754973.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54978
Clone ID Related Accession (Same CDS sequence)	XM_031899113.1
Accession Version	XM_031899113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATC ATAAAAATGT CATTCAGGGA ATAAAGAAGT ACCCAAATGT AACCTACCCA 
GTCCTTACTC CAAATCTTAC TGGATTTCAA GCTGCGGTTG AATGTGGTGC AAAGGAAGTA 
GCCATATTTG GAGCTGCATC AGAGCTCTTT AGCAAGAAGA ACATTAACTG TTCTATTGAT 
GAAAGTTTGC AAAGATTCAA GGATGTTATT GCAGCAGCTA AGGAAGCAAA CATCCCAGTG 
CGAGGGTATG TTTCATGTGT TCTTGGATGC CCATATGAAG GCAAGGTAGC TCCTAGTAAA 
GTGGCTGAAG TGGCATACAA GATGTTTTCT ATGGGCTGCT ATGAGATCTC TTTGGGTGAC 
ACGATTGGAG TGGGAACTCC CGGCGGTATG AGGGACATGC TGAGAGCTGT GTTGGATGTT 
GTTCCAGCCA AAGCTCTGGC TGTTCATTGC CATGACACAT ATGGACAGGC ACTGGCAAAT 
ATACTAGTTG CATTGCAGAT GGGAGTGCAG GTTGTTGATG CTTCAGTTGC AGGTTTGGGA 
GGATGCCCAT ATGCTCAGGG AGCTTCAGGG AATGTGGCAA CTGAAGATGT TGTGTATATG 
CTGCATGGTC TTGGAATTCA AACAGGTATT GACATAAAGA AACTGACAGA AGCTGGAGCT 
TTTATCTGCA AAGCACTAGG AAAGAAGAGC AACTCAAAAG TGGCTCAAGC AATGTGCAAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754973.1
CDS	302..1027
Translation	MADHKNVIQGIKKYPNVTYPVLTPNLTGFQAAVECGAKEVAIFGAASELFSKKNINCSIDESLQRFKDVIAAAKEANIPVRGYVSCVLGCPYEGKVAPSKVAEVAYKMFSMGCYEISLGDTIGVGTPGGMRDMLRAVLDVVPAKALAVHCHDTYGQALANILVALQMGVQVVDASVAGLGGCPYAQGASGNVATEDVVYMLHGLGIQTGIDIKKLTEAGAFICKALGKKSNSKVAQAMCKL

Target ORF information:

RefSeq Version	XM_031899113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA lyase (hmgcl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899113.1

ORF Insert Sequence:

ATGGCCGATC ATAAAAATGT CATTCAGGGA ATAAAGAAGT ACCCAAATGT AACCTACCCA 
GTCCTTACTC CAAATCTTAC TGGATTTCAA GCTGCGGTTG AATGTGGTGC AAAGGAAGTA 
GCCATATTTG GAGCTGCATC AGAGCTCTTT AGCAAGAAGA ACATTAACTG TTCTATTGAT 
GAAAGTTTGC AAAGATTCAA GGATGTTATT GCAGCAGCTA AGGAAGCAAA CATCCCAGTG 
CGAGGGTATG TTTCATGTGT TCTTGGATGC CCATATGAAG GCAAGGTAGC TCCTAGTAAA 
GTGGCTGAAG TGGCATACAA GATGTTTTCT ATGGGCTGCT ATGAGATCTC TTTGGGTGAC 
ACGATTGGAG TGGGAACTCC CGGCGGTATG AGGGACATGC TGAGAGCTGT GTTGGATGTT 
GTTCCAGCCA AAGCTCTGGC TGTTCATTGC CATGACACAT ATGGACAGGC ACTGGCAAAT 
ATACTAGTTG CATTGCAGAT GGGAGTGCAG GTTGTTGATG CTTCAGTTGC AGGTTTGGGA 
GGATGCCCAT ATGCTCAGGG AGCTTCAGGG AATGTGGCAA CTGAAGATGT TGTGTATATG 
CTGCATGGTC TTGGAATTCA AACAGGTATT GACATAAAGA AACTGACAGA AGCTGGAGCT 
TTTATCTGCA AAGCACTAGG AAAGAAGAGC AACTCAAAAG TGGCTCAAGC AATGTGCAAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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hmgcl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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