col27a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col27a1
Entrez Gene ID	100490770
Full Name	collagen, type XXVII, alpha 1
General protein information	Preferred Names collagen, type XXVII, alpha 1 Names collagen alpha-1(XXVII) chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096813.1	XP_017952302.1	collagen alpha-1(XXVII) chain isoform X4
XM_018096812.1	XP_017952301.1	collagen alpha-1(XXVII) chain isoform X3
XM_002935498.4	XP_002935544.2	collagen alpha-1(XXVII) chain isoform X2
XM_002935498.5	XP_002935544.2	collagen alpha-1(XXVII) chain isoform X2
XM_004916698.3	XP_004916755.1	collagen alpha-1(XXVII) chain isoform X1
XM_004916698.4	XP_004916755.1	collagen alpha-1(XXVII) chain isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL27A1	NP_001193609.1
Rattus norvegicus (Norway rat)	Col27a1	NP_942042.1
Homo sapiens (human)	COL27A1	NP_116277.2
Canis lupus familiaris (dog)	COL27A1	XP_005627059.1
Pan troglodytes (chimpanzee)	COL27A1	XP_520208.3
Gallus gallus (chicken)	COL27A1	XP_415514.3
Mus musculus (house mouse)	Col27a1	NP_079961.3
Xenopus tropicalis (tropical clawed frog)	LOC100490770	XP_004916755.1

Gene Ontology

The following col27a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col27a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14127	XM_004916698.4 Latest version!	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa14128	XM_002935498.5 Latest version!	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa46430	XM_018096812.1 Latest version!	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa46431	XM_018096813.1 Latest version!	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OXa14128	XM_002935498.4	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa14127	XM_004916698.3	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col27a1 ( XM_004916698.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916755.1 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.

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CloneID	OXa14127
Clone ID Related Accession (Same CDS sequence)	XM_004916698.3 , XM_004916698.4
Accession Version	XM_004916698.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916755.1
CDS	261..5351
Translation	MEPVSVPGFRLKAAAGGGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALMDSSGTSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGMTIHLEVGSVCFL

Target ORF information:

RefSeq Version	XM_004916698.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916698.3

ORF Insert Sequence:

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col27a1 ( XM_004916698.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916755.1 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.

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CloneID	OXa14127
Clone ID Related Accession (Same CDS sequence)	XM_004916698.3 , XM_004916698.4
Accession Version	XM_004916698.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916698.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916755.1
CDS	252..5342
Translation	MEPVSVPGFRLKAAAGGGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALMDSSGTSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGMTIHLEVGSVCFL

Target ORF information:

RefSeq Version	XM_004916698.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916698.4

ORF Insert Sequence:

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col27a1 ( XM_002935498.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935544.2 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa14128
Clone ID Related Accession (Same CDS sequence)	XM_002935498.4 , XM_002935498.5
Accession Version	XM_002935498.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAG GGGCTGGAAG GAGAGACTCC ACTTCTCACC TCACCACCAA GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935544.2
CDS	313..5403
Translation	MHLGAGRRDSTSHLTTKGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALMDSSGTSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGMTIHLEVGSVCFL

Target ORF information:

RefSeq Version	XM_002935498.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935498.4

ORF Insert Sequence:

ATGCATTTAG GGGCTGGAAG GAGAGACTCC ACTTCTCACC TCACCACCAA GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col27a1 ( XM_002935498.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935544.2 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.

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CloneID	OXa14128
Clone ID Related Accession (Same CDS sequence)	XM_002935498.4 , XM_002935498.5
Accession Version	XM_002935498.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935498.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTAG GGGCTGGAAG GAGAGACTCC ACTTCTCACC TCACCACCAA GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935544.2
CDS	347..5437
Translation	MHLGAGRRDSTSHLTTKGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALMDSSGTSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGMTIHLEVGSVCFL

Target ORF information:

RefSeq Version	XM_002935498.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935498.5

ORF Insert Sequence:

ATGCATTTAG GGGCTGGAAG GAGAGACTCC ACTTCTCACC TCACCACCAA GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGTGCAA GACGGACGCT GGCATCAGAC TGTTCTTATT 
TTTCGGACAC AGGACCCGAC TCAGTTCCCA ATTGTTGACG TGCTAAATCT ACCGCTACCA 
TCACCTGGCA TGACCATTCA TCTGGAAGTG GGAAGTGTCT GCTTCCTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col27a1 ( XM_018096812.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952301.1 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X3, mRNA.

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CloneID	OXa46430
Clone ID Related Accession (Same CDS sequence)	XM_018096812.1
Accession Version	XM_018096812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGGAAAA CAAAAATCTC TCCGTATAGC TGGAACGGAA 
GTGGAGATTG GCCTGAAAAC ACGAAAGCAG GCATTTTCGG GCAGGCCGAA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952301.1
CDS	262..5298
Translation	MEPVSVPGFRLKAAAGGGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALMDSSGTSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKGKQKSLRIAGTEVEIGLKTRKQAFSGRPKL

Target ORF information:

RefSeq Version	XM_018096812.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096812.1

ORF Insert Sequence:

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT AGGCAAGAGG ACTTTGCTGC TTCTTACCAG 
GCCCTCATGG ACTCCAGTGG AACATCCTAT CAGAGTACGA ACTACATGAT GGTGGACAAT 
GGATCAGAAA TATTTAAGAC CCTACACTAC CTCAGTAGCC TTATCCAGAG CATAAAAAGC 
CCACTTGGAA CGAGGGATAA CCCTGCTCGC ATCTGCAGAG ATTTGATGAA CTGCCAACAG 
AAAATGGGAG ATGGCACTTA TTGGATTGAC CCGAATATTG GCTGTTCTTC AGACACCATA 
GAAGTCACAT GCAATTTTAC AGAAGGTGGC CAAACCTGCC TTAAACCGAT CACAAAATCT 
AAAATGGAGT TTGAGGTTGG CAGGGTTCAG ATGAACTTCC TTCACCTGCT GAGTTCTGAG 
GCTATGCAGA GCATTACTTT TCACTGTCTG AATGTGCCAG CATGGGAGAA ATCTTCCAAG 
GCAGTACGAT TCAAAGCATG GAGTGGACAT ATATTTGGCA CAGAAGGCAT GTTTCAGCCA 
GAGGTGTCTG TGGATGGCTG CAAGGGAAAA CAAAAATCTC TCCGTATAGC TGGAACGGAA 
GTGGAGATTG GCCTGAAAAC ACGAAAGCAG GCATTTTCGG GCAGGCCGAA GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col27a1 ( XM_018096813.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952302.1 Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X4, mRNA.

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CloneID	OXa46431
Clone ID Related Accession (Same CDS sequence)	XM_018096813.1
Accession Version	XM_018096813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-1(XXVII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT TCCTATCAGA GTACGAACTA CATGATGGTG 
GACAATGGAT CAGAAATATT TAAGACCCTA CACTACCTCA GTAGCCTTAT CCAGAGCATA 
AAAAGCCCAC TTGGAACGAG GGATAACCCT GCTCGCATCT GCAGAGATTT GATGAACTGC 
CAACAGAAAA TGGGAGATGG CACTTATTGG ATTGACCCGA ATATTGGCTG TTCTTCAGAC 
ACCATAGAAG TCACATGCAA TTTTACAGAA GGTGGCCAAA CCTGCCTTAA ACCGATCACA 
AAATCTAAAA TGGAGTTTGA GGTTGGCAGG GTTCAGATGA ACTTCCTTCA CCTGCTGAGT 
TCTGAGGCTA TGCAGAGCAT TACTTTTCAC TGTCTGAATG TGCCAGCATG GGAGAAATCT 
TCCAAGGCAG TACGATTCAA AGCATGGAGT GGACATATAT TTGGCACAGA AGGCATGTTT 
CAGCCAGAGG TGTCTGTGGA TGGCTGCAAG GTGCAAGACG GACGCTGGCA TCAGACTGTT 
CTTATTTTTC GGACACAGGA CCCGACTCAG TTCCCAATTG TTGACGTGCT AAATCTACCG 
CTACCATCAC CTGGCATGAC CATTCATCTG GAAGTGGGAA GTGTCTGCTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952302.1
CDS	261..5297
Translation	MEPVSVPGFRLKAAAGGGTLFAWIVLYISCCLDISQGATEDVNVLQKLGLMGEKSSVRSVPQGVIPFKLGVILTQRARVEAPVHSILPPSLGTNLTLVLSLSSHRINNAFLFAVKSKKKKLELGVQFIPGKIIVYVGHKRSVYFDYNVHDGQWHNMALGIQGQTVTLYTSCGKQRVNASLHSRKVGPLDSDGVFILGKMNQHSIQFEGALCQFDIYPPAKAAHNYCKYLKKQCRQTDTYRPNLPHLIPLILNESRFHLGTEMPSRSVHNLTTILPRAELIGTSFSTSTKRIASGRLPFVKMTTKSPSLLASTSRPPYRIDTQLSSSTVTRTSTTVSGSSTSLPVNPLLEETVFNMAKSGLTPSQSTKKVYQKTTTKPSVTSAASKQVKKTTGSRRSTNTITPKQAVEKLNSGSSLKNHSYVDWKVKSNTSVLYTKPPSSSVPPSTHMVSASSPFAHDYYWLDPTPFPFLMGPPGPKGNQGSPGPPGLPGLTGSPGKRGPRGPPGPHGNPGPPGMHGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYSYQSTNYMMVDNGSEIFKTLHYLSSLIQSIKSPLGTRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKSKMEFEVGRVQMNFLHLLSSEAMQSITFHCLNVPAWEKSSKAVRFKAWSGHIFGTEGMFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGMTIHLEVGSVCFL

Target ORF information:

RefSeq Version	XM_018096813.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type XXVII, alpha 1 (col27a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096813.1

ORF Insert Sequence:

ATGGAGCCGG TATCAGTCCC GGGCTTCCGA CTGAAAGCAG CCGCTGGGGG GGGAACACTT 
TTTGCTTGGA TAGTTTTATA TATCTCCTGT TGCCTGGATA TTTCTCAGGG AGCTACTGAA 
GATGTTAACG TTCTTCAGAA GCTGGGCCTC ATGGGAGAGA AGTCATCTGT CCGCTCAGTG 
CCTCAGGGGG TCATCCCATT TAAGTTGGGG GTCATTTTGA CACAACGAGC AAGAGTGGAA 
GCTCCAGTTC ACTCCATTTT ACCGCCAAGC TTAGGAACAA ATCTCACTCT GGTGCTGAGC 
CTCAGCTCCC ACCGCATAAA TAATGCTTTT CTATTTGCCG TCAAAAGCAA GAAGAAGAAA 
CTCGAACTTG GGGTGCAGTT TATCCCAGGA AAAATTATAG TTTATGTGGG TCACAAGCGG 
TCTGTGTATT TTGATTACAA TGTTCACGAT GGCCAGTGGC ACAACATGGC CCTTGGTATT 
CAAGGCCAAA CGGTGACTCT ATATACTTCT TGCGGGAAGC AAAGAGTTAA TGCCAGTTTG 
CATTCAAGGA AGGTTGGCCC ATTGGATTCT GATGGGGTTT TTATACTTGG TAAAATGAAC 
CAGCACTCTA TTCAATTTGA AGGCGCCCTT TGCCAATTTG ATATTTATCC TCCAGCCAAG 
GCAGCTCATA ATTACTGTAA ATATCTTAAG AAGCAGTGCA GGCAGACAGA TACCTACAGG 
CCGAACCTAC CCCATCTAAT TCCGCTGATT CTTAATGAAA GCAGATTCCA TCTTGGCACT 
GAGATGCCTT CAAGATCTGT GCATAACCTT ACCACCATTT TACCCCGGGC AGAGTTGATT 
GGAACAAGCT TTAGTACCAG CACAAAAAGG ATAGCTTCTG GTAGGCTTCC TTTTGTAAAA 
ATGACTACCA AATCACCATC TTTGCTTGCC TCAACATCTC GGCCTCCTTA CAGAATTGAT 
ACACAGCTTT CTTCAAGCAC CGTAACAAGG ACTTCTACCA CTGTGTCCGG ATCATCAACA 
TCTCTGCCAG TTAATCCTCT GTTAGAAGAG ACTGTCTTCA ACATGGCAAA GTCAGGTCTA 
ACACCTAGTC AAAGCACCAA GAAGGTCTAT CAAAAAACAA CCACCAAGCC ATCAGTGACA 
TCTGCTGCTT CAAAGCAGGT TAAAAAGACC ACAGGGAGCA GAAGGAGTAC AAACACCATT 
ACCCCAAAAC AAGCTGTGGA GAAACTGAAT AGTGGGTCCA GTCTAAAGAA TCACTCTTAT 
GTGGACTGGA AGGTTAAATC AAACACTTCT GTACTTTACA CCAAACCACC ATCCTCTTCT 
GTTCCACCAT CAACTCACAT GGTTTCAGCC AGTTCTCCCT TTGCTCACGA TTACTATTGG 
CTTGATCCAA CACCATTTCC ATTTCTGATG GGACCTCCAG GCCCCAAAGG CAATCAGGGT 
AGTCCAGGTC CTCCTGGTTT ACCTGGTCTC ACTGGCTCAC CTGGGAAAAG AGGCCCACGG 
GGTCCGCCTG GTCCTCATGG AAATCCTGGC CCACCTGGTA TGCATGGCCC AAAGGGGCAA 
AAGGGTGATC CTGGGCTTTC ACCAGGAAAA GCAATAAATG GATTAAAGGG AGATCCTGGT 
TTGCCTGGTT TAACTGGAGT TCCTGGGCCA CTAAGTCGTA AGGGACAGAA AGGCTATCCA 
GGACCTCAAG GACATCCTGG TGAACCGGGT GTGCGAGGAC CCGAGGGAAG CCCAGGGGCC 
AAAGGTTATC CTGGCAGGCA GGGCTTGCCT GGACCAATAG GAGTTTCTGG GCCCAAGGGA 
AACCGGGGCT ACATTGGCCC CCCTGGTTTG TTTGGGCTGC CTGGCGCTGA CGGAGAACGT 
GGTTTGCCTG GAGTTCCCGG GAAGAGGGGC ACCATGGGTA GGCCGGGATT CCCTGGTGAT 
TTTGGAGAGA GGGGACCTCC CGGGTTGGAT GGAAACCCAG GTGAATTAGG AGCCCCAGGT 
CCCCCCGGTG TTCCTGGATT CATAGGCGAT ATGGGTGCTG TTGGAGCTGT AGGATTTCCG 
GGACTCAAAG GTTTAAAGGG GCTGCCGGGG AACTCTGGAG AATCTGGACT GAAAGGTGAT 
AAGGGCATTC AGGGATTTCC AGGGATACCC GGAGACATTG GCTTTCAAGG AGACAAGGGC 
AGTCAGGGTC TTCCAGGGCC ACCTGGAATG CGTGGGAAAA CTGGTGTAAT GGGGAAACCA 
GGCGATAAGG GTTTTGCGGG ATTCCCGGGC CCTCCAGGAC CAGAGGGCTT TCCTGGGGAC 
ATTGGGCCTC CGGGAGAAAA CGGTCCAGAA GGTATCAAGG GAAAGCCAGG AGCCAGAGGG 
TTCCCAGGAC CTAGAGGACA GCCAGGGCAT GAGGGGGATG AGGGACCAAT TGGGCCACCA 
GGAGCTCCAG GTCTGGAGGG GTTTCCTGGG CGGAAGGGCT ATCCTGGGAT TCTTGGGGCA 
GAAGGTCCAA AGGGTGAAGC AGGCAACACA GGAAGGTCTG GGAAGATCGG TGAACAGGGG 
CTTCAAGGAT TTATTGGTCC AGTGGGAGAG ACAGGAATTG CAGGGGAGAA GGGAGATCGT 
GGGGGAATTG GGAGTCCAGG ACCACCAGGG GAAAAGGGAA CAATGGGTCA TCCTGGGAAT 
CCAGGACCTC CAGGTTCTCC GGGCCCTCCT GGACCACCGG GTTCACCTGG ATCAAGGGGT 
TCTCCGGGTG TGCGTGGTCC AAAAGGACGC AGAGGTGCTA GAGGCCCTGA TGGACCAGCG 
GGAGAACCTG GACTTGCAGG TACTAAGGGT CCTTCTGGGA TACAGGGCAA GCAAGGTCTG 
ATTGGCCCAC AGGGCAAAAT GGGAGAGAGA GGAATACCAG GGCCAAATGG TTTTCCAGGA 
ATATCAGGAC CTTCCGGCCC ACCCGGGGAG AAGGGAATTA CAGGAAATCC AGGCCCCGCT 
GGAGCACAAG GACCTGTAGG CGCTTTGGGA GAAATTGGAC ACAAGGGACC TCAAGGAGCA 
AAGGGTGAGC CAGGACCTCC AGGTGAAACT GGATTAAAGG GAGACCTTGG TCCATTGGGC 
ATTCCAGGAG AACAAGGTCT GATTGGTCAA AGGGGAGACC CAGGATTAGA GGGGGAGAGT 
GGCTTAACTG GCCCTGATGG CATGAAGGGA GAAATTGGAC AACCCGGACC TGAGGGGGAA 
AAGGGAGAGA AAGGAGTGGA AGGAACAAAA GGAAAAGATG GACCCCCCGG TCCATCTGGA 
ATTCCAGGTG TCAGAGGCGT TGAAGGAAAG CCAGGAAAAC AAGGAGAAAA AGGAAAACCT 
GGGCAAAAGG GGAGCAAAGG CTTTGAGGGC CATTTGGGAG AAACCGGTGT TCCAGGAGAT 
CAAGGGCCCA CTGGGAACCA AGGACCTAAG GGAAGCAGGG GCACTCGTGG ACCCGTTGGA 
TCCCCTGGGA GAATGGGTGC ACAGGGTGAG CCAGGAATGC CAGGCTATCA GGGACATAAG 
GGACCCCCCG GTCCACTAGG ACCACCAGGA ATAAAAGGTG AAAAGGGAGA ACAAGGGGAT 
GATGGGAAGG CAGAAGGTAC CCCTGGAGCT CCTGGAGACA GAGGCCCTGT TGGTGATGGT 
GGAGACAGAG GAGAGCCTGG TGACCCCGGA TATCCTGGTC AAGACGGACC GGAGGGACCT 
AGAGGAAATG CTGGGCAGCA GGGTTTACCG GGTCATTCTG GACCACGAGG TCTTCAGGGC 
TTACGAGGAT CCAAAGGCAC TGTGGGTTTG AAGGGCAAGC CTGGAAAGCC AGGGGAGTCG 
GGGGCACATG GACAGCCTGG TGCTATAGGT GTGACTGGTC CAAGGGGAGT AGTAGGAAGA 
CAGGGCCAGG AGGGCCCTGC TGGACCTGAT GGGATTCCAG GAAAAGATGG CAGTCCCGGA 
CTTAAGGGAG AACAAGGAGA TGATGGGGAA ATTGGCCTTG CTGGAACTTT AGGAAAACGA 
GGAAATACTG GTGTTCCCGG TTTGCCTGGA GCTCAAGGAC CACCAGGATT CAGGGGTGAA 
AGAGGATTGC CTGGTGAAAT TGGACTGCCA GGAAAACGTG GCATGGAAGG CGGAATGGGA 
CTTTCAGGAG CCAAAGGGGA GCTTGGGACA AAAGGCCAGC CTGGTGACCC TGGGGAGATG 
GGGTTTCCAG GCATTGCCGG ACTCTTTGGA CCCAAGGGAC CCCCAGGTGA TGTCGGATTT 
AAGGGCATTC AGGGACCAAA AGGACCTTTA GGTTTAATGG GAAAGGAAGG TGCTGGCGGA 
CCTATGGGTA TTGTTGGACC AAATGGAAAC CCTGGGCCTG TCGGAGACAG AGGAAGCCAA 
GGGGATAATG GAATGCCAGG TCCTCGGGGC CCTCCTGGTC CAAGGGGAAA GCCTGGTCCA 
CCGGGCCCTT CAGTGATCCT TGATTCATAT TCCTATCAGA GTACGAACTA CATGATGGTG 
GACAATGGAT CAGAAATATT TAAGACCCTA CACTACCTCA GTAGCCTTAT CCAGAGCATA 
AAAAGCCCAC TTGGAACGAG GGATAACCCT GCTCGCATCT GCAGAGATTT GATGAACTGC 
CAACAGAAAA TGGGAGATGG CACTTATTGG ATTGACCCGA ATATTGGCTG TTCTTCAGAC 
ACCATAGAAG TCACATGCAA TTTTACAGAA GGTGGCCAAA CCTGCCTTAA ACCGATCACA 
AAATCTAAAA TGGAGTTTGA GGTTGGCAGG GTTCAGATGA ACTTCCTTCA CCTGCTGAGT 
TCTGAGGCTA TGCAGAGCAT TACTTTTCAC TGTCTGAATG TGCCAGCATG GGAGAAATCT 
TCCAAGGCAG TACGATTCAA AGCATGGAGT GGACATATAT TTGGCACAGA AGGCATGTTT 
CAGCCAGAGG TGTCTGTGGA TGGCTGCAAG GTGCAAGACG GACGCTGGCA TCAGACTGTT 
CTTATTTTTC GGACACAGGA CCCGACTCAG TTCCCAATTG TTGACGTGCT AAATCTACCG 
CTACCATCAC CTGGCATGAC CATTCATCTG GAAGTGGGAA GTGTCTGCTT CCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col27a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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