Col27a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col27a1
Entrez Gene ID	298101
Full Name	collagen type XXVII alpha 1 chain
General protein information	Preferred Names collagen type XXVII alpha 1 chain Names collagen alpha-1(XXVII) chain collagen type XXVII proalpha 1 chain collagen, type XXVII, alpha 1 procollagen, type XXVII, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q24
Summary	mouse homolog is a fibrillar collagen gene; may be a component of the extracellular matrices of cartilage [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006238245.3	XP_006238307.1	collagen alpha-1(XXVII) chain isoform X1
XM_017593250.1	XP_017448739.1	collagen alpha-1(XXVII) chain isoform X2
NM_198747.1	NP_942042.1	collagen alpha-1(XXVII) chain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL27A1	NP_001193609.1
Rattus norvegicus (Norway rat)	Col27a1	NP_942042.1
Homo sapiens (human)	COL27A1	NP_116277.2
Canis lupus familiaris (dog)	COL27A1	XP_005627059.1
Pan troglodytes (chimpanzee)	COL27A1	XP_520208.3
Gallus gallus (chicken)	COL27A1	XP_415514.3
Mus musculus (house mouse)	Col27a1	NP_079961.3
Xenopus tropicalis (tropical clawed frog)	LOC100490770	XP_004916755.1

Gene Ontology
Bibliography

Related articles in PubMed

Collagen XXVII organises the pericellular matrix in the growth plate.
Plumb DA, Ferrara L, Torbica T, Knowles L, Mironov A Jr, Kadler KE, Briggs MD, Boot-Handford RP
PloS one6(12)e29422(2011)

Collagen XXVII is developmentally regulated and forms thin fibrillar structures distinct from those of classical vertebrate fibrillar collagens.
Plumb DA, Dhir V, Mironov A, Ferrara L, Poulsom R, Kadler KE, Thornton DJ, Briggs MD, Boot-Handford RP
The Journal of biological chemistry282(17)12791-5(2007 Apr)

Identification, characterization and expression analysis of a new fibrillar collagen gene, COL27A1.
Pace JM, Corrado M, Missero C, Byers PH
Matrix biology : journal of the International Society for Matrix Biology22(1)3-14(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Col27a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col27a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42505	NM_198747.1 Latest version!	Rattus norvegicus collagen type XXVII alpha 1 chain (Col27a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa28225	XM_006238245.3 Latest version!	Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61150	XM_017593250.1 Latest version!	Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col27a1 ( NM_198747.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942042.1 Rattus norvegicus collagen type XXVII alpha 1 chain (Col27a1), mRNA.

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CloneID	ORa42505
Clone ID Related Accession (Same CDS sequence)	NM_198747.1
Accession Version	NM_198747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XXVII) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY232999.1. On Dec 17, 2003 this sequence version replaced XM_216414.2. ##Evidence-Data-START## Transcript exon combination :: AY232999.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGCCTGG CGCGGGCGAC CGCGGGGCTG GGGCCGTGCT GTCCGCCTGC TCCGGCGCTC 
CTGGGCGCAG GGCTGCGCTG GGGGGGATTT CTCTTCGCCT GGATCTTGGT CTCATTTTCC 
TGTCATCTGG CCTCCACCCA AGGAGCTCCT GAAGATGTGG ATGTCCTCCA GCGGCTGGGC 
CTCAGCTGGA CGAAAGCTGG GGGTGGCCGG AGCCCCGCAC CCCCTGGTGT CATTCCTTTC 
CCATCTGGCT TCATCTTCAC ACAGCGGGCC AAGCTGCAGG CCCCTACTAC CAATGTCCTT 
CCCACCACCC TGGGGCGGGA GCTGGCATTG GTGTTGAGCC TCTGTTCGCA CCGTGTGAAC 
CATGCCTTCC TCTTCGCCAT CCGCAGCAGG AAACACAGGC TGCAGCTGGG TCTGCAGTTC 
CTCCCCGGCA GGACTCTCGT CCACCTGGGA CCTCGGCAAT CTGTGGCCTT CGACTTGGAT 
GTGCATGATG GGCGCTGGCA CCACCTGGCC CTAGAGTTGC GTGGTCGCAC GGTCACATTG 
GTGACAGCCT GTGGGCAGCA CAGGGTACCT GTTCCGCTGC CTTCCCGTAG GGACTCCATG 
CTTGACCCCC AGGGCTCCTT TCTCTTGGGG AAGATGAACC CCCGAGCTGT CCAGTTTGAA 
GGTGCACTCT GTCAGTTCAG CATCCATCCC GTGGCGCAGG TCGCTCACAA TTACTGTGCC 
CACCTGAGAG AACGGTGCCG ACAGGTGGAC ACGTATGGTC CCCAGGTGGG AGCCCTCTTT 
CCCTGGGACT CTGGCCCAGC CTTTGCTTTG CATCCTGAAC CAGCCTTGCT TGGTCTGGGG 
AACCTGACCA GAAACCCAGC AACCCTAGGG TCCAGGCCTA TAAGCAGGGG ACTCATGGTG 
ACTATGGCTC CTGCCGTGCC CACCAAGCCC CTAAGGATGG TCCACCAAGA TGTCTCCAAA 
CTCGGCTCTT CTCAGACCCC ATTGGTCCCT GCCAAACAGT CAGCCAGAAA AACACCTTCC 
CCCTTCCCTT CAGCTGCTCT GGCTAATTCC ACCAGGGTTT TTCACTCAGC TCCTGCCCAG 
CCACGACAAA TCACAGCTAC TAGCCCCACC AAACGTCCCC CGACCAAGCC TTCTGTTTCT 
TCCCTTTCAG TTACCCCCAT GAAAAGTCCC CAGGCAATCC AGAAAGCAGG TACCCCTTCA 
TTCTCAAGGC CCATTCCAAC CACCCAGAAG CCAACACCCC TCACCTCCCA CCCATCACCC 
TCCAAAGTCT CTAGTGCCAC TGTGAGGCCT GTCCAGAAGA CTTTCATGAC ACCCCAACCT 
CCAACCCTGA GTCCCCAAGC CCTGCACCCT ATTACTGGCT TACCTAAGAA GTTCACTATC 
CCCACAGTAG CAAAACCTCA GTCCAAGATG ACCAGTTGGG CCAGCAAGCC AGTCTTGGCC 
CGCACCAATG TCCCTAAGGC TCTTGAGCAA ACTGTTGTGG CCCAGTCCTC TGTTTCCTAT 
CTGGGCTCTC AGACACTGGC TACAGCCCTC CCTCCTCTGG GTGTTGGAAA TTCTAGAATG 
ATGCCTTCCA CACGTGACTC TACTTCAACT CCTGCTGGAA GCAAGAAAAT CACAGGATTG 
GAAGCTTCCA AGAAAACCAG ACATAAGAGC AGCCCCCGGA AGCCCATACC TCTCAGCTCT 
GGGAAGACAG CCAGGGATGC TTCACCAAGA GACCTGACAA CCAAGCCTAG CCAGCTGTCC 
ACTCCAGCCC TGGTCCTTGC CCCAGCCCAC CTCCTGTCCT CCAGCCCCCA GCCCACCAGC 
AGCAGCTTTT CTTTCTTCCA CCTGCCGGAA CCTACACCTT TCCTGATGCT GATGGGGCCT 
CCTGGCTCCA AGGGAGACTG TGGCTTGCCG GGCCCTCCTG GGCTTCCAGG ATTACCTGGA 
TCTCCGGGTC CACGAGGTCC TCGGGGTCCT CCTGGGCCAT TTGGGAACCC AGGTCTACCT 
GGACCTCCTG GAGCCAAAGG ACAGAAAGGG GACCCGGGAC TCTCACCAGG ACAGGCTCAC 
GATGGGGCAA AGGGCAACAT GGGCTTGCCT GGGCTCGCCG GAAATCCAGG ACCCATGGGA 
CGGAAGGGAC ACAAGGGCCA CCCTGGAGCG GCAGGGCACC CTGGAGAACA GGGGCAGCCA 
GGACCTGAGG GCAGTCCAGG GGCCAAAGGT TACCCTGGCA GGCAGGGGTT CCCTGGACCT 
GTAGGAGACC CTGGCCCCAA AGGCAGCAGG GGTTACATTG GGCTTCCGGG GCTCTTTGGC 
CTGCCAGGGT CGGATGGAGA GCGGGGTCTC CCCGGCATTC CTGGCAAGAG GGGCGAGATG 
GGTAGGCCGG GCTTCCCTGG AGACTTTGGG GAAAGAGGTC CCCCTGGACT TGATGGGAAC 
CCTGGGGAAA TTGGCCTGCC AGGACCACCA GGAGTGCTCG GGCTCCTTGG TGACATGGGA 
GCATTAGGTC CAGTTGGCTA CCCGGGACCA AAAGGCATGA AGGGACTGAT GGGCGGCGTG 
GGGGAGCCCG GACTGAAAGG TGATAAGGGT GAACAAGGGG TGCCAGGTGT ATCGGGAGAT 
CCTGGCTTCC AAGGAGACAA GGGGAGTCAT GGCTTGCCAG GGTTCCCAGG TGCCCGGGGG 
AAGCCAGGGC CTATGGGCAA AGCTGGGGAC AAAGGCTCAC TTGGGCTTCC TGGACCTCCT 
GGTCCTGAGG GTTTCCCAGG AGACATTGGC CCCCCAGGGG ACAATGGCCC TGAAGGCATG 
AAGGGTAAGC CTGGAGCCCG AGGCCTACCT GGACCTCCTG GGCAGCTGGG GCCTGAGGGA 
GATGAAGGAC CCATGGGGCC ACCAGGAGTC CCAGGCTTGG AGGGTCAACC TGGAAGGAAG 
GGGTTCCCGG GGAGGCCTGG CCTGGACGGC TCGAAGGGGG AACCAGGGGA TCCTGGACGG 
CCAGGGCCTG TGGGTGAACA GGGGCTTATG GGATTTGTTG GTCTGGTTGG AGAGCCTGGA 
ATTGTTGGGG AGAAGGGAGA CCGTGGAGTG ATGGGACCCC CAGGGGCACC TGGACCCAAG 
GGGTCGATGG GCCATCCTGG AACACCTGGT GGTGTTGGGG ACCCTGGAGA GCCTGGACCC 
TGGGGCCCTC CAGGATCTCG AGGACTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC 
CCTCGAGGAC CTGATGGACC AGCTGGGGAG CAGGGGTCCA AGGGCCTGAA GGGTCGTGTA 
GGGCCTCGGG GCAGACCTGG CCAGCCAGGA CAGCAGGGTG CAGCTGGCGA GCGAGGCCAC 
TCAGGTGCAA AAGGCTTCCT TGGCATTCCT GGTCCCTCAG GCCCTCCAGG TGCCAAGGGC 
CTCCCAGGAG AACCGGGCTC TCAGGGACCT CAGGGGCCAG TTGGTCCTCC AGGAGAGATG 
GGACCCAAGG GGCCGCCTGG TGCAGTGGGA GAACCTGGCC TTCCTGGGGA CTCCGGTATG 
AAGGGTGACC TTGGACCTCT GGGCCCTCCT GGGGAACAAG GGCTCATTGG GCAACGGGGA 
GAGCCAGGCC TCGAGGGAGA CCTTGGCCCA GTGGGGCCAG ATGGGCTGAA GGGGGACAGG 
GGTGACCCAG GTCCTGATGG TGAACATGGT GAAAAGGGTC AGGAAGGACT GAAAGGAGAG 
GAGGGGCTCC CTGGGCCCCC AGGCATCACT GGTGTCCGGG GTCGTGAAGG GAAGCCTGGC 
AGTCAGGGTG AGAAGGGCCA GAGGGGAGCT AAGGGTGCCA AGGGCTACCA AGGACAGCTG 
GGAGAGATGG GCATCCCTGG AGACCCTGGC CCCCCTGGCA CCCCAGGTCC TAAAGGGTCC 
CGGGGCACCC TAGGACCAAT GGGTGCTCCA GGACGGATGG GAGCCCAAGG AGAACCAGGA 
TTGGCTGGTT ATAATGGGCA TAAAGGCATC ACGGGACCTC TCGGGCCTCC CGGGCCCAAA 
GGCGAGAAGG GGGAACAGGG CGAGGATGGC AAGACTGAGG GGGCCCCCGG GCCACCAGGA 
GAGCGGGGTC CTGTGGGTGA TCGAGGAGAC CGTGGGGAGC CAGGCGACCC TGGATACCCT 
GGTCAAGAGG GTGTTCAAGG CCTCCGTGGA GAACCAGGCC AGCAGGGCCA GCCCGGGCAT 
CCAGGACCCC GGGGACGCCC AGGACCCAAA GGATCAAAAG GCGAAGAGGG CCCAAAGGGA 
AAGCCAGGCA AGGCGGGGGC ATCAGGCCGG AGGGGGACCC AGGGGCTTCA AGGGCTGCCA 
GGGCCGAGAG GTGTAGTAGG GAGACAGGGC CCTGAGGGCA TGGCTGGACA AGATGGGAAT 
CCTGGCAGGG ACGGTCGGCC AGGATATCAG GGAGAGCAGG GAAATGATGG GGACCCTGGC 
CCCGTGGGCC CTGCTGGGAG AAGAGGGAAT CCAGGTGTGG CTGGCTTGCC TGGAGCACAG 
GGGCCTCCAG GATTCAAGGG TGAAAGTGGG TTACCTGGGC AACTGGGTCC CCCTGGAAAA 
CGAGGGACAG AAGGTGGAAC GGGGCTTCCT GGGAACCAGG GGGAGCCAGG ATCCAAAGGC 
CAGCCGGGTG ACTCTGGCGA GATGGGCTTC CCAGGAGTGG CTGGCCTCTT TGGACCCAAG 
GGTCCCCCTG GAGACATTGG CTTCAAAGGC ATCCAAGGCC CTCGGGGTCC TCCTGGCTTG 
ATGGGAAAGG AAGGTATCAT TGGGCCCCCC GGAATGCTGG GACCTTCTGG ACTCCCGGGT 
CCCAAAGGTG ACAGAGGCAG CCGAGGGGAC TGGGGACTGC AAGGCCCAAG GGGTCCTCCT 
GGTCCGAGGG GGCGGCCAGG TCCACCGGGC CCTCCTTGGC ATCCTGTCCA GTTTCAACAA 
GATGACCTTG AGGCAGCTTT CCAAACGTGG ATGGATGCTC ACGGAGCAGT CAGATTGGAG 
CAGGGGTACA GCTATCCGGA CCAGCTGATG CTAGACCAGG GAGGGGAGAT CTTCAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCTTGGGCAC CAAGGAGAAC 
CCAGCCCGGG TCTGCCGGGA CCTCATGGAC TGCGAACAGA AGATGGCGGA TGGTATCTAC 
TGGGTGGACC CCAACCTCGG CTGCTCCTCT GATACCATCG AAGTCTCCTG CAACTTTACA 
CATGGTGGGC AGACTTGCCT GAAGCCTATC ACGGCCTCCA AGGCAGAGTT TGCTGTTAGT 
CGGGTCCAGA TGAATTTCTT GCACCTGCTC AGCTCTGAGG GGACACAGCA CATCACAATC 
CACTGCCTGA ACATGACGGT GTGGCAGGAG GGACCTGCAC GCCCCTCTGC CAGGCAAGCT 
GTGCGCTTCC GTGCCTGGAA CGGACAGGTC TTTGAAGCTG GGGGTCAATT CAGGCCAGAG 
GTGTCTATGG ATGGCTGCAA GGTCCATGAT GGCCGCTGGC ATCAGACACT ATTCACCTTC 
CGGACCCAGG ACCCCCAGCA GCTGCCCATT GTCAGTGTGG ACAACCTTCC ACCTGTCTCG 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCATGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942042.1
CDS	1..5568
Translation	MGLARATAGLGPCCPPAPALLGAGLRWGGFLFAWILVSFSCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPAPPGVIPFPSGFIFTQRAKLQAPTTNVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHRLQLGLQFLPGRTLVHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTLVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKMNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYGPQVGALFPWDSGPAFALHPEPALLGLGNLTRNPATLGSRPISRGLMVTMAPAVPTKPLRMVHQDVSKLGSSQTPLVPAKQSARKTPSPFPSAALANSTRVFHSAPAQPRQITATSPTKRPPTKPSVSSLSVTPMKSPQAIQKAGTPSFSRPIPTTQKPTPLTSHPSPSKVSSATVRPVQKTFMTPQPPTLSPQALHPITGLPKKFTIPTVAKPQSKMTSWASKPVLARTNVPKALEQTVVAQSSVSYLGSQTLATALPPLGVGNSRMMPSTRDSTSTPAGSKKITGLEASKKTRHKSSPRKPIPLSSGKTARDASPRDLTTKPSQLSTPALVLAPAHLLSSSPQPTSSSFSFFHLPEPTPFLMLMGPPGSKGDCGLPGPPGLPGLPGSPGPRGPRGPPGPFGNPGLPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGTLGPMGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGEQGEDGKTEGAPGPPGERGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRPGYQGEQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDWGLQGPRGPPGPRGRPGPPGPPWHPVQFQQDDLEAAFQTWMDAHGAVRLEQGYSYPDQLMLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMADGIYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPARPSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	NM_198747.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XXVII alpha 1 chain (Col27a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198747.1

ORF Insert Sequence:

ATGGGCCTGG CGCGGGCGAC CGCGGGGCTG GGGCCGTGCT GTCCGCCTGC TCCGGCGCTC 
CTGGGCGCAG GGCTGCGCTG GGGGGGATTT CTCTTCGCCT GGATCTTGGT CTCATTTTCC 
TGTCATCTGG CCTCCACCCA AGGAGCTCCT GAAGATGTGG ATGTCCTCCA GCGGCTGGGC 
CTCAGCTGGA CGAAAGCTGG GGGTGGCCGG AGCCCCGCAC CCCCTGGTGT CATTCCTTTC 
CCATCTGGCT TCATCTTCAC ACAGCGGGCC AAGCTGCAGG CCCCTACTAC CAATGTCCTT 
CCCACCACCC TGGGGCGGGA GCTGGCATTG GTGTTGAGCC TCTGTTCGCA CCGTGTGAAC 
CATGCCTTCC TCTTCGCCAT CCGCAGCAGG AAACACAGGC TGCAGCTGGG TCTGCAGTTC 
CTCCCCGGCA GGACTCTCGT CCACCTGGGA CCTCGGCAAT CTGTGGCCTT CGACTTGGAT 
GTGCATGATG GGCGCTGGCA CCACCTGGCC CTAGAGTTGC GTGGTCGCAC GGTCACATTG 
GTGACAGCCT GTGGGCAGCA CAGGGTACCT GTTCCGCTGC CTTCCCGTAG GGACTCCATG 
CTTGACCCCC AGGGCTCCTT TCTCTTGGGG AAGATGAACC CCCGAGCTGT CCAGTTTGAA 
GGTGCACTCT GTCAGTTCAG CATCCATCCC GTGGCGCAGG TCGCTCACAA TTACTGTGCC 
CACCTGAGAG AACGGTGCCG ACAGGTGGAC ACGTATGGTC CCCAGGTGGG AGCCCTCTTT 
CCCTGGGACT CTGGCCCAGC CTTTGCTTTG CATCCTGAAC CAGCCTTGCT TGGTCTGGGG 
AACCTGACCA GAAACCCAGC AACCCTAGGG TCCAGGCCTA TAAGCAGGGG ACTCATGGTG 
ACTATGGCTC CTGCCGTGCC CACCAAGCCC CTAAGGATGG TCCACCAAGA TGTCTCCAAA 
CTCGGCTCTT CTCAGACCCC ATTGGTCCCT GCCAAACAGT CAGCCAGAAA AACACCTTCC 
CCCTTCCCTT CAGCTGCTCT GGCTAATTCC ACCAGGGTTT TTCACTCAGC TCCTGCCCAG 
CCACGACAAA TCACAGCTAC TAGCCCCACC AAACGTCCCC CGACCAAGCC TTCTGTTTCT 
TCCCTTTCAG TTACCCCCAT GAAAAGTCCC CAGGCAATCC AGAAAGCAGG TACCCCTTCA 
TTCTCAAGGC CCATTCCAAC CACCCAGAAG CCAACACCCC TCACCTCCCA CCCATCACCC 
TCCAAAGTCT CTAGTGCCAC TGTGAGGCCT GTCCAGAAGA CTTTCATGAC ACCCCAACCT 
CCAACCCTGA GTCCCCAAGC CCTGCACCCT ATTACTGGCT TACCTAAGAA GTTCACTATC 
CCCACAGTAG CAAAACCTCA GTCCAAGATG ACCAGTTGGG CCAGCAAGCC AGTCTTGGCC 
CGCACCAATG TCCCTAAGGC TCTTGAGCAA ACTGTTGTGG CCCAGTCCTC TGTTTCCTAT 
CTGGGCTCTC AGACACTGGC TACAGCCCTC CCTCCTCTGG GTGTTGGAAA TTCTAGAATG 
ATGCCTTCCA CACGTGACTC TACTTCAACT CCTGCTGGAA GCAAGAAAAT CACAGGATTG 
GAAGCTTCCA AGAAAACCAG ACATAAGAGC AGCCCCCGGA AGCCCATACC TCTCAGCTCT 
GGGAAGACAG CCAGGGATGC TTCACCAAGA GACCTGACAA CCAAGCCTAG CCAGCTGTCC 
ACTCCAGCCC TGGTCCTTGC CCCAGCCCAC CTCCTGTCCT CCAGCCCCCA GCCCACCAGC 
AGCAGCTTTT CTTTCTTCCA CCTGCCGGAA CCTACACCTT TCCTGATGCT GATGGGGCCT 
CCTGGCTCCA AGGGAGACTG TGGCTTGCCG GGCCCTCCTG GGCTTCCAGG ATTACCTGGA 
TCTCCGGGTC CACGAGGTCC TCGGGGTCCT CCTGGGCCAT TTGGGAACCC AGGTCTACCT 
GGACCTCCTG GAGCCAAAGG ACAGAAAGGG GACCCGGGAC TCTCACCAGG ACAGGCTCAC 
GATGGGGCAA AGGGCAACAT GGGCTTGCCT GGGCTCGCCG GAAATCCAGG ACCCATGGGA 
CGGAAGGGAC ACAAGGGCCA CCCTGGAGCG GCAGGGCACC CTGGAGAACA GGGGCAGCCA 
GGACCTGAGG GCAGTCCAGG GGCCAAAGGT TACCCTGGCA GGCAGGGGTT CCCTGGACCT 
GTAGGAGACC CTGGCCCCAA AGGCAGCAGG GGTTACATTG GGCTTCCGGG GCTCTTTGGC 
CTGCCAGGGT CGGATGGAGA GCGGGGTCTC CCCGGCATTC CTGGCAAGAG GGGCGAGATG 
GGTAGGCCGG GCTTCCCTGG AGACTTTGGG GAAAGAGGTC CCCCTGGACT TGATGGGAAC 
CCTGGGGAAA TTGGCCTGCC AGGACCACCA GGAGTGCTCG GGCTCCTTGG TGACATGGGA 
GCATTAGGTC CAGTTGGCTA CCCGGGACCA AAAGGCATGA AGGGACTGAT GGGCGGCGTG 
GGGGAGCCCG GACTGAAAGG TGATAAGGGT GAACAAGGGG TGCCAGGTGT ATCGGGAGAT 
CCTGGCTTCC AAGGAGACAA GGGGAGTCAT GGCTTGCCAG GGTTCCCAGG TGCCCGGGGG 
AAGCCAGGGC CTATGGGCAA AGCTGGGGAC AAAGGCTCAC TTGGGCTTCC TGGACCTCCT 
GGTCCTGAGG GTTTCCCAGG AGACATTGGC CCCCCAGGGG ACAATGGCCC TGAAGGCATG 
AAGGGTAAGC CTGGAGCCCG AGGCCTACCT GGACCTCCTG GGCAGCTGGG GCCTGAGGGA 
GATGAAGGAC CCATGGGGCC ACCAGGAGTC CCAGGCTTGG AGGGTCAACC TGGAAGGAAG 
GGGTTCCCGG GGAGGCCTGG CCTGGACGGC TCGAAGGGGG AACCAGGGGA TCCTGGACGG 
CCAGGGCCTG TGGGTGAACA GGGGCTTATG GGATTTGTTG GTCTGGTTGG AGAGCCTGGA 
ATTGTTGGGG AGAAGGGAGA CCGTGGAGTG ATGGGACCCC CAGGGGCACC TGGACCCAAG 
GGGTCGATGG GCCATCCTGG AACACCTGGT GGTGTTGGGG ACCCTGGAGA GCCTGGACCC 
TGGGGCCCTC CAGGATCTCG AGGACTGCCA GGCATGAGGG GAGCCAAGGG GCACCGGGGC 
CCTCGAGGAC CTGATGGACC AGCTGGGGAG CAGGGGTCCA AGGGCCTGAA GGGTCGTGTA 
GGGCCTCGGG GCAGACCTGG CCAGCCAGGA CAGCAGGGTG CAGCTGGCGA GCGAGGCCAC 
TCAGGTGCAA AAGGCTTCCT TGGCATTCCT GGTCCCTCAG GCCCTCCAGG TGCCAAGGGC 
CTCCCAGGAG AACCGGGCTC TCAGGGACCT CAGGGGCCAG TTGGTCCTCC AGGAGAGATG 
GGACCCAAGG GGCCGCCTGG TGCAGTGGGA GAACCTGGCC TTCCTGGGGA CTCCGGTATG 
AAGGGTGACC TTGGACCTCT GGGCCCTCCT GGGGAACAAG GGCTCATTGG GCAACGGGGA 
GAGCCAGGCC TCGAGGGAGA CCTTGGCCCA GTGGGGCCAG ATGGGCTGAA GGGGGACAGG 
GGTGACCCAG GTCCTGATGG TGAACATGGT GAAAAGGGTC AGGAAGGACT GAAAGGAGAG 
GAGGGGCTCC CTGGGCCCCC AGGCATCACT GGTGTCCGGG GTCGTGAAGG GAAGCCTGGC 
AGTCAGGGTG AGAAGGGCCA GAGGGGAGCT AAGGGTGCCA AGGGCTACCA AGGACAGCTG 
GGAGAGATGG GCATCCCTGG AGACCCTGGC CCCCCTGGCA CCCCAGGTCC TAAAGGGTCC 
CGGGGCACCC TAGGACCAAT GGGTGCTCCA GGACGGATGG GAGCCCAAGG AGAACCAGGA 
TTGGCTGGTT ATAATGGGCA TAAAGGCATC ACGGGACCTC TCGGGCCTCC CGGGCCCAAA 
GGCGAGAAGG GGGAACAGGG CGAGGATGGC AAGACTGAGG GGGCCCCCGG GCCACCAGGA 
GAGCGGGGTC CTGTGGGTGA TCGAGGAGAC CGTGGGGAGC CAGGCGACCC TGGATACCCT 
GGTCAAGAGG GTGTTCAAGG CCTCCGTGGA GAACCAGGCC AGCAGGGCCA GCCCGGGCAT 
CCAGGACCCC GGGGACGCCC AGGACCCAAA GGATCAAAAG GCGAAGAGGG CCCAAAGGGA 
AAGCCAGGCA AGGCGGGGGC ATCAGGCCGG AGGGGGACCC AGGGGCTTCA AGGGCTGCCA 
GGGCCGAGAG GTGTAGTAGG GAGACAGGGC CCTGAGGGCA TGGCTGGACA AGATGGGAAT 
CCTGGCAGGG ACGGTCGGCC AGGATATCAG GGAGAGCAGG GAAATGATGG GGACCCTGGC 
CCCGTGGGCC CTGCTGGGAG AAGAGGGAAT CCAGGTGTGG CTGGCTTGCC TGGAGCACAG 
GGGCCTCCAG GATTCAAGGG TGAAAGTGGG TTACCTGGGC AACTGGGTCC CCCTGGAAAA 
CGAGGGACAG AAGGTGGAAC GGGGCTTCCT GGGAACCAGG GGGAGCCAGG ATCCAAAGGC 
CAGCCGGGTG ACTCTGGCGA GATGGGCTTC CCAGGAGTGG CTGGCCTCTT TGGACCCAAG 
GGTCCCCCTG GAGACATTGG CTTCAAAGGC ATCCAAGGCC CTCGGGGTCC TCCTGGCTTG 
ATGGGAAAGG AAGGTATCAT TGGGCCCCCC GGAATGCTGG GACCTTCTGG ACTCCCGGGT 
CCCAAAGGTG ACAGAGGCAG CCGAGGGGAC TGGGGACTGC AAGGCCCAAG GGGTCCTCCT 
GGTCCGAGGG GGCGGCCAGG TCCACCGGGC CCTCCTTGGC ATCCTGTCCA GTTTCAACAA 
GATGACCTTG AGGCAGCTTT CCAAACGTGG ATGGATGCTC ACGGAGCAGT CAGATTGGAG 
CAGGGGTACA GCTATCCGGA CCAGCTGATG CTAGACCAGG GAGGGGAGAT CTTCAAAACC 
TTACACTACC TCAGCAACCT CATCCAGAGC ATTAAGACGC CCTTGGGCAC CAAGGAGAAC 
CCAGCCCGGG TCTGCCGGGA CCTCATGGAC TGCGAACAGA AGATGGCGGA TGGTATCTAC 
TGGGTGGACC CCAACCTCGG CTGCTCCTCT GATACCATCG AAGTCTCCTG CAACTTTACA 
CATGGTGGGC AGACTTGCCT GAAGCCTATC ACGGCCTCCA AGGCAGAGTT TGCTGTTAGT 
CGGGTCCAGA TGAATTTCTT GCACCTGCTC AGCTCTGAGG GGACACAGCA CATCACAATC 
CACTGCCTGA ACATGACGGT GTGGCAGGAG GGACCTGCAC GCCCCTCTGC CAGGCAAGCT 
GTGCGCTTCC GTGCCTGGAA CGGACAGGTC TTTGAAGCTG GGGGTCAATT CAGGCCAGAG 
GTGTCTATGG ATGGCTGCAA GGTCCATGAT GGCCGCTGGC ATCAGACACT ATTCACCTTC 
CGGACCCAGG ACCCCCAGCA GCTGCCCATT GTCAGTGTGG ACAACCTTCC ACCTGTCTCG 
TCAGGGAAGC AGTACCGCCT GGAAGTTGGA CCTGCATGCT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_006238245.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238307.1 Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

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CloneID	ORa28225
Clone ID Related Accession (Same CDS sequence)	XM_006238245.3
Accession Version	XM_006238245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XXVII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGG GATCGGCGAG GGGGGCCCGA GGCACAGCGG CGACAGGATG CGGGGGGGGA 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT TCCTGTCATC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAAGC TGGGGGTGGC 
CGGAGCCCCG CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCTAC TACCAATGTC CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC GCACCGTGTG AACCATGCCT TCCTCTTCGC CATCCGCAGC 
AGGAAACACA GGCTGCAGCT GGGTCTGCAG TTCCTCCCCG GCAGGACTCT CGTCCACCTG 
GGACCTCGGC AATCTGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGTCG CACGGTCACA TTGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTTCCGC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGATGA ACCCCCGAGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAT 
CCCGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAACGGTG CCGACAGGTG 
GACACGTATG GTCCCCAGGT GGGAGCCCTC TTTCCCTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGTCTG GGGAACCTGA CCAGAAACCC AGCAACCCTA 
GGGTCCAGGC CTATAAGCAG GGGACTCATG GTGACTATGG CTCCTGCCGT GCCCACCAAG 
CCCCTAAGGA TGGTCCACCA AGATGTCTCC AAACTCGGCT CTTCTCAGAC CCCATTGGTC 
CCTGCCAAAC AGTCAGCCAG AAAAACACCT TCCCCCTTCC CTTCAGCTGC TCTGGCTAAT 
TCCACCAGGG TTTTTCACTC AGCTCCTGCC CAGCCACGAC AAATCACAGC TACTAGCCCC 
ACCAAACGTC CCCCGACCAA GCCTTCTGTT TCTTCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCAGGCAA TCCAGAAAGC AGGTACCCCT TCATTCTCAA GGCCCATTCC AACCACCCAG 
AAGCCAACAC CCCTCACCTC CCACCCATCA CCCTCCAAAG TCTCTAGTGC CACTGTGAGG 
CCTGTCCAGA AGACTTTCAT GACACCCCAA CCTCCAACCC TGAGTCCCCA AGCCCTGCAC 
CCTATTACTG GCTTACCTAA GAAGTTCACT ATCCCCACAG TAGCAAAACC TCAGTCCAAG 
ATGACCAGTT GGGCCAGCAA GCCAGTCTTG GCCCGCACCA ATGTCCCTAA GGCTCTTGAG 
CAAACTGTTG TGGCCCAGTC CTCTGTTTCC TATCTGGGCT CTCAGACACT GGCTACAGCC 
CTCCCTCCTC TGGGTGTTGG AAATTCTAGA ATGATGCCTT CCACACGTGA CTCTACTTCA 
ACTCCTGCTG GAAGCAAGAA AATCACAGGA TTGGAAGCTT CCAAGAAAAC CAGACATAAG 
AGCAGCCCCC GGAAGCCCAT ACCTCTCAGC TCTGGGAAGA CAGCCAGGGA TGCTTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCAGCTG TCCACTCCAG CCCTGGTCCT TGCCCCAGCC 
CACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TTTCTTTCTT CCACCTGCCG 
GAACCTACAC CTTTCCTGAT GCTGATGGGG CCTCCTGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGCCCTC CTGGGCTTCC AGGATTACCT GGATCTCCGG GTCCACGAGG TCCTCGGGGT 
CCTCCTGGGC CATTTGGGAA CCCAGGTCTA CCTGGACCTC CTGGAGCCAA AGGACAGAAA 
GGGGACCCGG GACTCTCACC AGGACAGGCT CACGATGGGG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCG CCGGAAATCC AGGACCCATG GGACGGAAGG GACACAAGGG CCACCCTGGA 
GCGGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGGCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCCGGCA TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GTCCCCCTGG ACTTGATGGG AACCCTGGGG AAATTGGCCT GCCAGGACCA 
CCAGGAGTGC TCGGGCTCCT TGGTGACATG GGAGCATTAG GTCCAGTTGG CTACCCGGGA 
CCAAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTATCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGTTCCC AGGTGCCCGG GGGAAGCCAG GGCCTATGGG CAAAGCTGGG 
GACAAAGGCT CACTTGGGCT TCCTGGACCT CCTGGTCCTG AGGGTTTCCC AGGAGACATT 
GGCCCCCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTA 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA ACCTGGAAGG AAGGGGTTCC CGGGGAGGCC TGGCCTGGAC 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGGC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTTG TTGGTCTGGT TGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGA 
GTGATGGGAC CCCCAGGGGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCT 
GGTGGTGTTG GGGACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGACTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGGT CCAAGGGCCT GAAGGGTCGT GTAGGGCCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG CGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATT 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAG ATGGGACCCA AGGGGCCGCC TGGTGCAGTG 
GGAGAACCTG GCCTTCCTGG GGACTCCGGT ATGAAGGGTG ACCTTGGACC TCTGGGCCCT 
CCTGGGGAAC AAGGGCTCAT TGGGCAACGG GGAGAGCCAG GCCTCGAGGG AGACCTTGGC 
CCAGTGGGGC CAGATGGGCT GAAGGGGGAC AGGGGTGACC CAGGTCCTGA TGGTGAACAT 
GGTGAAAAGG GTCAGGAAGG ACTGAAAGGA GAGGAGGGGC TCCCTGGGCC CCCAGGCATC 
ACTGGTGTCC GGGGTCGTGA AGGGAAGCCT GGCAGTCAGG GTGAGAAGGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCTA CCAAGGACAG CTGGGAGAGA TGGGCATCCC TGGAGACCCT 
GGCCCCCCTG GCACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AATGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCTG GTTATAATGG GCATAAAGGC 
ATCACGGGAC CTCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGAACA GGGCGAGGAT 
GGCAAGACTG AGGGGGCCCC CGGGCCACCA GGAGAGCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGTGTTCA AGGCCTCCGT 
GGAGAACCAG GCCAGCAGGG CCAGCCCGGG CATCCAGGAC CCCGGGGACG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCGGG GGCATCAGGC 
CGGAGGGGGA CCCAGGGGCT TCAAGGGCTG CCAGGGCCGA GAGGTGTAGT AGGGAGACAG 
GGCCCTGAGG GCATGGCTGG ACAAGATGGG AATCCTGGCA GGGACGGTCG GCCAGGATAT 
CAGGGAGAGC AGGGAAATGA TGGGGACCCT GGCCCCGTGG GCCCTGCTGG GAGAAGAGGG 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CAGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACGGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGTCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GCCCTCGGGG TCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGGCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGG 
GACTGGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCGA GGGGGCGGCC AGGTCCACCG 
GGCCCTCCTT GGCATCCTGT CCAGTTTCAA CAAGATGACC TTGAGGCAGC TTTCCAAACG 
TGGATGGATG CTCACGGAGC AGTCAGATTG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
ATGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CGCCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGCGAAC AGAAGATGGC GGATGGTATC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGATACCA TCGAAGTCTC CTGCAACTTT ACACATGGTG GGCAGACTTG CCTGAAGCCT 
ATCACGGCCT CCAAGGCAGA GTTTGCTGTT AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTCAGCTCTG AGGGGACACA GCACATCACA ATCCACTGCC TGAACATGAC GGTGTGGCAG 
GAGGGACCTG CACGCCCCTC TGCCAGGCAA GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTTGAAG CTGGGGGTCA ATTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTATTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATTGTCAGTG TGGACAACCT TCCACCTGTC TCGTCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCAT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238307.1
CDS	996..6536
Translation	MGTGSARGARGTAATGCGGGFLFAWILVSFSCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPAPPGVIPFPSGFIFTQRAKLQAPTTNVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHRLQLGLQFLPGRTLVHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTLVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKMNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYGPQVGALFPWDSGPAFALHPEPALLGLGNLTRNPATLGSRPISRGLMVTMAPAVPTKPLRMVHQDVSKLGSSQTPLVPAKQSARKTPSPFPSAALANSTRVFHSAPAQPRQITATSPTKRPPTKPSVSSLSVTPMKSPQAIQKAGTPSFSRPIPTTQKPTPLTSHPSPSKVSSATVRPVQKTFMTPQPPTLSPQALHPITGLPKKFTIPTVAKPQSKMTSWASKPVLARTNVPKALEQTVVAQSSVSYLGSQTLATALPPLGVGNSRMMPSTRDSTSTPAGSKKITGLEASKKTRHKSSPRKPIPLSSGKTARDASPRDLTTKPSQLSTPALVLAPAHLLSSSPQPTSSSFSFFHLPEPTPFLMLMGPPGSKGDCGLPGPPGLPGLPGSPGPRGPRGPPGPFGNPGLPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGTLGPMGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGEQGEDGKTEGAPGPPGERGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRPGYQGEQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDWGLQGPRGPPGPRGRPGPPGPPWHPVQFQQDDLEAAFQTWMDAHGAVRLEQGYSYPDQLMLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMADGIYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPARPSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_006238245.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238245.3

ORF Insert Sequence:

ATGGGCACGG GATCGGCGAG GGGGGCCCGA GGCACAGCGG CGACAGGATG CGGGGGGGGA 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT TCCTGTCATC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAAGC TGGGGGTGGC 
CGGAGCCCCG CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCTAC TACCAATGTC CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC GCACCGTGTG AACCATGCCT TCCTCTTCGC CATCCGCAGC 
AGGAAACACA GGCTGCAGCT GGGTCTGCAG TTCCTCCCCG GCAGGACTCT CGTCCACCTG 
GGACCTCGGC AATCTGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGTCG CACGGTCACA TTGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTTCCGC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGATGA ACCCCCGAGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAT 
CCCGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAACGGTG CCGACAGGTG 
GACACGTATG GTCCCCAGGT GGGAGCCCTC TTTCCCTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGTCTG GGGAACCTGA CCAGAAACCC AGCAACCCTA 
GGGTCCAGGC CTATAAGCAG GGGACTCATG GTGACTATGG CTCCTGCCGT GCCCACCAAG 
CCCCTAAGGA TGGTCCACCA AGATGTCTCC AAACTCGGCT CTTCTCAGAC CCCATTGGTC 
CCTGCCAAAC AGTCAGCCAG AAAAACACCT TCCCCCTTCC CTTCAGCTGC TCTGGCTAAT 
TCCACCAGGG TTTTTCACTC AGCTCCTGCC CAGCCACGAC AAATCACAGC TACTAGCCCC 
ACCAAACGTC CCCCGACCAA GCCTTCTGTT TCTTCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCAGGCAA TCCAGAAAGC AGGTACCCCT TCATTCTCAA GGCCCATTCC AACCACCCAG 
AAGCCAACAC CCCTCACCTC CCACCCATCA CCCTCCAAAG TCTCTAGTGC CACTGTGAGG 
CCTGTCCAGA AGACTTTCAT GACACCCCAA CCTCCAACCC TGAGTCCCCA AGCCCTGCAC 
CCTATTACTG GCTTACCTAA GAAGTTCACT ATCCCCACAG TAGCAAAACC TCAGTCCAAG 
ATGACCAGTT GGGCCAGCAA GCCAGTCTTG GCCCGCACCA ATGTCCCTAA GGCTCTTGAG 
CAAACTGTTG TGGCCCAGTC CTCTGTTTCC TATCTGGGCT CTCAGACACT GGCTACAGCC 
CTCCCTCCTC TGGGTGTTGG AAATTCTAGA ATGATGCCTT CCACACGTGA CTCTACTTCA 
ACTCCTGCTG GAAGCAAGAA AATCACAGGA TTGGAAGCTT CCAAGAAAAC CAGACATAAG 
AGCAGCCCCC GGAAGCCCAT ACCTCTCAGC TCTGGGAAGA CAGCCAGGGA TGCTTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCAGCTG TCCACTCCAG CCCTGGTCCT TGCCCCAGCC 
CACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TTTCTTTCTT CCACCTGCCG 
GAACCTACAC CTTTCCTGAT GCTGATGGGG CCTCCTGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGCCCTC CTGGGCTTCC AGGATTACCT GGATCTCCGG GTCCACGAGG TCCTCGGGGT 
CCTCCTGGGC CATTTGGGAA CCCAGGTCTA CCTGGACCTC CTGGAGCCAA AGGACAGAAA 
GGGGACCCGG GACTCTCACC AGGACAGGCT CACGATGGGG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCG CCGGAAATCC AGGACCCATG GGACGGAAGG GACACAAGGG CCACCCTGGA 
GCGGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGGCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCCGGCA TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GTCCCCCTGG ACTTGATGGG AACCCTGGGG AAATTGGCCT GCCAGGACCA 
CCAGGAGTGC TCGGGCTCCT TGGTGACATG GGAGCATTAG GTCCAGTTGG CTACCCGGGA 
CCAAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTATCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGTTCCC AGGTGCCCGG GGGAAGCCAG GGCCTATGGG CAAAGCTGGG 
GACAAAGGCT CACTTGGGCT TCCTGGACCT CCTGGTCCTG AGGGTTTCCC AGGAGACATT 
GGCCCCCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTA 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA ACCTGGAAGG AAGGGGTTCC CGGGGAGGCC TGGCCTGGAC 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGGC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTTG TTGGTCTGGT TGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGA 
GTGATGGGAC CCCCAGGGGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCT 
GGTGGTGTTG GGGACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGACTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGGT CCAAGGGCCT GAAGGGTCGT GTAGGGCCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG CGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATT 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAG ATGGGACCCA AGGGGCCGCC TGGTGCAGTG 
GGAGAACCTG GCCTTCCTGG GGACTCCGGT ATGAAGGGTG ACCTTGGACC TCTGGGCCCT 
CCTGGGGAAC AAGGGCTCAT TGGGCAACGG GGAGAGCCAG GCCTCGAGGG AGACCTTGGC 
CCAGTGGGGC CAGATGGGCT GAAGGGGGAC AGGGGTGACC CAGGTCCTGA TGGTGAACAT 
GGTGAAAAGG GTCAGGAAGG ACTGAAAGGA GAGGAGGGGC TCCCTGGGCC CCCAGGCATC 
ACTGGTGTCC GGGGTCGTGA AGGGAAGCCT GGCAGTCAGG GTGAGAAGGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCTA CCAAGGACAG CTGGGAGAGA TGGGCATCCC TGGAGACCCT 
GGCCCCCCTG GCACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AATGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCTG GTTATAATGG GCATAAAGGC 
ATCACGGGAC CTCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGAACA GGGCGAGGAT 
GGCAAGACTG AGGGGGCCCC CGGGCCACCA GGAGAGCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGTGTTCA AGGCCTCCGT 
GGAGAACCAG GCCAGCAGGG CCAGCCCGGG CATCCAGGAC CCCGGGGACG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCGGG GGCATCAGGC 
CGGAGGGGGA CCCAGGGGCT TCAAGGGCTG CCAGGGCCGA GAGGTGTAGT AGGGAGACAG 
GGCCCTGAGG GCATGGCTGG ACAAGATGGG AATCCTGGCA GGGACGGTCG GCCAGGATAT 
CAGGGAGAGC AGGGAAATGA TGGGGACCCT GGCCCCGTGG GCCCTGCTGG GAGAAGAGGG 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CAGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACGGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGTCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GCCCTCGGGG TCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGGCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGG 
GACTGGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCGA GGGGGCGGCC AGGTCCACCG 
GGCCCTCCTT GGCATCCTGT CCAGTTTCAA CAAGATGACC TTGAGGCAGC TTTCCAAACG 
TGGATGGATG CTCACGGAGC AGTCAGATTG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
ATGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CGCCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGCGAAC AGAAGATGGC GGATGGTATC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGATACCA TCGAAGTCTC CTGCAACTTT ACACATGGTG GGCAGACTTG CCTGAAGCCT 
ATCACGGCCT CCAAGGCAGA GTTTGCTGTT AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTCAGCTCTG AGGGGACACA GCACATCACA ATCCACTGCC TGAACATGAC GGTGTGGCAG 
GAGGGACCTG CACGCCCCTC TGCCAGGCAA GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTTGAAG CTGGGGGTCA ATTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTATTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATTGTCAGTG TGGACAACCT TCCACCTGTC TCGTCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCAT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col27a1 ( XM_017593250.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448739.1 Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

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CloneID	ORa61150
Clone ID Related Accession (Same CDS sequence)	XM_017593250.1
Accession Version	XM_017593250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XXVII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTAG GGACCCGCCT AAGAACCGAC CGGCCAGCGT CGCACTTAGG GACCCAGGGA 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT TCCTGTCATC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAAGC TGGGGGTGGC 
CGGAGCCCCG CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCTAC TACCAATGTC CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC GCACCGTGTG AACCATGCCT TCCTCTTCGC CATCCGCAGC 
AGGAAACACA GGCTGCAGCT GGGTCTGCAG TTCCTCCCCG GCAGGACTCT CGTCCACCTG 
GGACCTCGGC AATCTGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGTCG CACGGTCACA TTGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTTCCGC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGATGA ACCCCCGAGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAT 
CCCGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAACGGTG CCGACAGGTG 
GACACGTATG GTCCCCAGGT GGGAGCCCTC TTTCCCTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGTCTG GGGAACCTGA CCAGAAACCC AGCAACCCTA 
GGGTCCAGGC CTATAAGCAG GGGACTCATG GTGACTATGG CTCCTGCCGT GCCCACCAAG 
CCCCTAAGGA TGGTCCACCA AGATGTCTCC AAACTCGGCT CTTCTCAGAC CCCATTGGTC 
CCTGCCAAAC AGTCAGCCAG AAAAACACCT TCCCCCTTCC CTTCAGCTGC TCTGGCTAAT 
TCCACCAGGG TTTTTCACTC AGCTCCTGCC CAGCCACGAC AAATCACAGC TACTAGCCCC 
ACCAAACGTC CCCCGACCAA GCCTTCTGTT TCTTCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCAGGCAA TCCAGAAAGC AGGTACCCCT TCATTCTCAA GGCCCATTCC AACCACCCAG 
AAGCCAACAC CCCTCACCTC CCACCCATCA CCCTCCAAAG TCTCTAGTGC CACTGTGAGG 
CCTGTCCAGA AGACTTTCAT GACACCCCAA CCTCCAACCC TGAGTCCCCA AGCCCTGCAC 
CCTATTACTG GCTTACCTAA GAAGTTCACT ATCCCCACAG TAGCAAAACC TCAGTCCAAG 
ATGACCAGTT GGGCCAGCAA GCCAGTCTTG GCCCGCACCA ATGTCCCTAA GGCTCTTGAG 
CAAACTGTTG TGGCCCAGTC CTCTGTTTCC TATCTGGGCT CTCAGACACT GGCTACAGCC 
CTCCCTCCTC TGGGTGTTGG AAATTCTAGA ATGATGCCTT CCACACGTGA CTCTACTTCA 
ACTCCTGCTG GAAGCAAGAA AATCACAGGA TTGGAAGCTT CCAAGAAAAC CAGACATAAG 
AGCAGCCCCC GGAAGCCCAT ACCTCTCAGC TCTGGGAAGA CAGCCAGGGA TGCTTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCAGCTG TCCACTCCAG CCCTGGTCCT TGCCCCAGCC 
CACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TTTCTTTCTT CCACCTGCCG 
GAACCTACAC CTTTCCTGAT GCTGATGGGG CCTCCTGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGCCCTC CTGGGCTTCC AGGATTACCT GGATCTCCGG GTCCACGAGG TCCTCGGGGT 
CCTCCTGGGC CATTTGGGAA CCCAGGTCTA CCTGGACCTC CTGGAGCCAA AGGACAGAAA 
GGGGACCCGG GACTCTCACC AGGACAGGCT CACGATGGGG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCG CCGGAAATCC AGGACCCATG GGACGGAAGG GACACAAGGG CCACCCTGGA 
GCGGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGGCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCCGGCA TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GTCCCCCTGG ACTTGATGGG AACCCTGGGG AAATTGGCCT GCCAGGACCA 
CCAGGAGTGC TCGGGCTCCT TGGTGACATG GGAGCATTAG GTCCAGTTGG CTACCCGGGA 
CCAAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTATCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGTTCCC AGGTGCCCGG GGGAAGCCAG GGCCTATGGG CAAAGCTGGG 
GACAAAGGCT CACTTGGGCT TCCTGGACCT CCTGGTCCTG AGGGTTTCCC AGGAGACATT 
GGCCCCCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTA 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA ACCTGGAAGG AAGGGGTTCC CGGGGAGGCC TGGCCTGGAC 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGGC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTTG TTGGTCTGGT TGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGA 
GTGATGGGAC CCCCAGGGGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCT 
GGTGGTGTTG GGGACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGACTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGGT CCAAGGGCCT GAAGGGTCGT GTAGGGCCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG CGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATT 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAG ATGGGACCCA AGGGGCCGCC TGGTGCAGTG 
GGAGAACCTG GCCTTCCTGG GGACTCCGGT ATGAAGGGTG ACCTTGGACC TCTGGGCCCT 
CCTGGGGAAC AAGGGCTCAT TGGGCAACGG GGAGAGCCAG GCCTCGAGGG AGACCTTGGC 
CCAGTGGGGC CAGATGGGCT GAAGGGGGAC AGGGGTGACC CAGGTCCTGA TGGTGAACAT 
GGTGAAAAGG GTCAGGAAGG ACTGAAAGGA GAGGAGGGGC TCCCTGGGCC CCCAGGCATC 
ACTGGTGTCC GGGGTCGTGA AGGGAAGCCT GGCAGTCAGG GTGAGAAGGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCTA CCAAGGACAG CTGGGAGAGA TGGGCATCCC TGGAGACCCT 
GGCCCCCCTG GCACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AATGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCTG GTTATAATGG GCATAAAGGC 
ATCACGGGAC CTCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGAACA GGGCGAGGAT 
GGCAAGACTG AGGGGGCCCC CGGGCCACCA GGAGAGCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGTGTTCA AGGCCTCCGT 
GGAGAACCAG GCCAGCAGGG CCAGCCCGGG CATCCAGGAC CCCGGGGACG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCGGG GGCATCAGGC 
CGGAGGGGGA CCCAGGGGCT TCAAGGGCTG CCAGGGCCGA GAGGTGTAGT AGGGAGACAG 
GGCCCTGAGG GCATGGCTGG ACAAGATGGG AATCCTGGCA GGGACGGTCG GCCAGGATAT 
CAGGGAGAGC AGGGAAATGA TGGGGACCCT GGCCCCGTGG GCCCTGCTGG GAGAAGAGGG 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CAGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACGGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGTCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GCCCTCGGGG TCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGGCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGG 
GACTGGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCGA GGGGGCGGCC AGGTCCACCG 
GGCCCTCCTT GGCATCCTGT CCAGTTTCAA CAAGATGACC TTGAGGCAGC TTTCCAAACG 
TGGATGGATG CTCACGGAGC AGTCAGATTG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
ATGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CGCCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGCGAAC AGAAGATGGC GGATGGTATC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGATACCA TCGAAGTCTC CTGCAACTTT ACACATGGTG GGCAGACTTG CCTGAAGCCT 
ATCACGGCCT CCAAGGCAGA GTTTGCTGTT AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTCAGCTCTG AGGGGACACA GCACATCACA ATCCACTGCC TGAACATGAC GGTGTGGCAG 
GAGGGACCTG CACGCCCCTC TGCCAGGCAA GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTTGAAG CTGGGGGTCA ATTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTATTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATTGTCAGTG TGGACAACCT TCCACCTGTC TCGTCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCAT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448739.1
CDS	239..5779
Translation	MPLGTRLRTDRPASHLGTQGFLFAWILVSFSCHLASTQGAPEDVDVLQRLGLSWTKAGGGRSPAPPGVIPFPSGFIFTQRAKLQAPTTNVLPTTLGRELALVLSLCSHRVNHAFLFAIRSRKHRLQLGLQFLPGRTLVHLGPRQSVAFDLDVHDGRWHHLALELRGRTVTLVTACGQHRVPVPLPSRRDSMLDPQGSFLLGKMNPRAVQFEGALCQFSIHPVAQVAHNYCAHLRERCRQVDTYGPQVGALFPWDSGPAFALHPEPALLGLGNLTRNPATLGSRPISRGLMVTMAPAVPTKPLRMVHQDVSKLGSSQTPLVPAKQSARKTPSPFPSAALANSTRVFHSAPAQPRQITATSPTKRPPTKPSVSSLSVTPMKSPQAIQKAGTPSFSRPIPTTQKPTPLTSHPSPSKVSSATVRPVQKTFMTPQPPTLSPQALHPITGLPKKFTIPTVAKPQSKMTSWASKPVLARTNVPKALEQTVVAQSSVSYLGSQTLATALPPLGVGNSRMMPSTRDSTSTPAGSKKITGLEASKKTRHKSSPRKPIPLSSGKTARDASPRDLTTKPSQLSTPALVLAPAHLLSSSPQPTSSSFSFFHLPEPTPFLMLMGPPGSKGDCGLPGPPGLPGLPGSPGPRGPRGPPGPFGNPGLPGPPGAKGQKGDPGLSPGQAHDGAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGTLGPMGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGEQGEDGKTEGAPGPPGERGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRPGYQGEQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDWGLQGPRGPPGPRGRPGPPGPPWHPVQFQQDDLEAAFQTWMDAHGAVRLEQGYSYPDQLMLDQGGEIFKTLHYLSNLIQSIKTPLGTKENPARVCRDLMDCEQKMADGIYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKAEFAVSRVQMNFLHLLSSEGTQHITIHCLNMTVWQEGPARPSARQAVRFRAWNGQVFEAGGQFRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSGKQYRLEVGPACFL

Target ORF information:

RefSeq Version	XM_017593250.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type XXVII, alpha 1 (Col27a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593250.1

ORF Insert Sequence:

ATGCCGTTAG GGACCCGCCT AAGAACCGAC CGGCCAGCGT CGCACTTAGG GACCCAGGGA 
TTTCTCTTCG CCTGGATCTT GGTCTCATTT TCCTGTCATC TGGCCTCCAC CCAAGGAGCT 
CCTGAAGATG TGGATGTCCT CCAGCGGCTG GGCCTCAGCT GGACGAAAGC TGGGGGTGGC 
CGGAGCCCCG CACCCCCTGG TGTCATTCCT TTCCCATCTG GCTTCATCTT CACACAGCGG 
GCCAAGCTGC AGGCCCCTAC TACCAATGTC CTTCCCACCA CCCTGGGGCG GGAGCTGGCA 
TTGGTGTTGA GCCTCTGTTC GCACCGTGTG AACCATGCCT TCCTCTTCGC CATCCGCAGC 
AGGAAACACA GGCTGCAGCT GGGTCTGCAG TTCCTCCCCG GCAGGACTCT CGTCCACCTG 
GGACCTCGGC AATCTGTGGC CTTCGACTTG GATGTGCATG ATGGGCGCTG GCACCACCTG 
GCCCTAGAGT TGCGTGGTCG CACGGTCACA TTGGTGACAG CCTGTGGGCA GCACAGGGTA 
CCTGTTCCGC TGCCTTCCCG TAGGGACTCC ATGCTTGACC CCCAGGGCTC CTTTCTCTTG 
GGGAAGATGA ACCCCCGAGC TGTCCAGTTT GAAGGTGCAC TCTGTCAGTT CAGCATCCAT 
CCCGTGGCGC AGGTCGCTCA CAATTACTGT GCCCACCTGA GAGAACGGTG CCGACAGGTG 
GACACGTATG GTCCCCAGGT GGGAGCCCTC TTTCCCTGGG ACTCTGGCCC AGCCTTTGCT 
TTGCATCCTG AACCAGCCTT GCTTGGTCTG GGGAACCTGA CCAGAAACCC AGCAACCCTA 
GGGTCCAGGC CTATAAGCAG GGGACTCATG GTGACTATGG CTCCTGCCGT GCCCACCAAG 
CCCCTAAGGA TGGTCCACCA AGATGTCTCC AAACTCGGCT CTTCTCAGAC CCCATTGGTC 
CCTGCCAAAC AGTCAGCCAG AAAAACACCT TCCCCCTTCC CTTCAGCTGC TCTGGCTAAT 
TCCACCAGGG TTTTTCACTC AGCTCCTGCC CAGCCACGAC AAATCACAGC TACTAGCCCC 
ACCAAACGTC CCCCGACCAA GCCTTCTGTT TCTTCCCTTT CAGTTACCCC CATGAAAAGT 
CCCCAGGCAA TCCAGAAAGC AGGTACCCCT TCATTCTCAA GGCCCATTCC AACCACCCAG 
AAGCCAACAC CCCTCACCTC CCACCCATCA CCCTCCAAAG TCTCTAGTGC CACTGTGAGG 
CCTGTCCAGA AGACTTTCAT GACACCCCAA CCTCCAACCC TGAGTCCCCA AGCCCTGCAC 
CCTATTACTG GCTTACCTAA GAAGTTCACT ATCCCCACAG TAGCAAAACC TCAGTCCAAG 
ATGACCAGTT GGGCCAGCAA GCCAGTCTTG GCCCGCACCA ATGTCCCTAA GGCTCTTGAG 
CAAACTGTTG TGGCCCAGTC CTCTGTTTCC TATCTGGGCT CTCAGACACT GGCTACAGCC 
CTCCCTCCTC TGGGTGTTGG AAATTCTAGA ATGATGCCTT CCACACGTGA CTCTACTTCA 
ACTCCTGCTG GAAGCAAGAA AATCACAGGA TTGGAAGCTT CCAAGAAAAC CAGACATAAG 
AGCAGCCCCC GGAAGCCCAT ACCTCTCAGC TCTGGGAAGA CAGCCAGGGA TGCTTCACCA 
AGAGACCTGA CAACCAAGCC TAGCCAGCTG TCCACTCCAG CCCTGGTCCT TGCCCCAGCC 
CACCTCCTGT CCTCCAGCCC CCAGCCCACC AGCAGCAGCT TTTCTTTCTT CCACCTGCCG 
GAACCTACAC CTTTCCTGAT GCTGATGGGG CCTCCTGGCT CCAAGGGAGA CTGTGGCTTG 
CCGGGCCCTC CTGGGCTTCC AGGATTACCT GGATCTCCGG GTCCACGAGG TCCTCGGGGT 
CCTCCTGGGC CATTTGGGAA CCCAGGTCTA CCTGGACCTC CTGGAGCCAA AGGACAGAAA 
GGGGACCCGG GACTCTCACC AGGACAGGCT CACGATGGGG CAAAGGGCAA CATGGGCTTG 
CCTGGGCTCG CCGGAAATCC AGGACCCATG GGACGGAAGG GACACAAGGG CCACCCTGGA 
GCGGCAGGGC ACCCTGGAGA ACAGGGGCAG CCAGGACCTG AGGGCAGTCC AGGGGCCAAA 
GGTTACCCTG GCAGGCAGGG GTTCCCTGGA CCTGTAGGAG ACCCTGGCCC CAAAGGCAGC 
AGGGGTTACA TTGGGCTTCC GGGGCTCTTT GGCCTGCCAG GGTCGGATGG AGAGCGGGGT 
CTCCCCGGCA TTCCTGGCAA GAGGGGCGAG ATGGGTAGGC CGGGCTTCCC TGGAGACTTT 
GGGGAAAGAG GTCCCCCTGG ACTTGATGGG AACCCTGGGG AAATTGGCCT GCCAGGACCA 
CCAGGAGTGC TCGGGCTCCT TGGTGACATG GGAGCATTAG GTCCAGTTGG CTACCCGGGA 
CCAAAAGGCA TGAAGGGACT GATGGGCGGC GTGGGGGAGC CCGGACTGAA AGGTGATAAG 
GGTGAACAAG GGGTGCCAGG TGTATCGGGA GATCCTGGCT TCCAAGGAGA CAAGGGGAGT 
CATGGCTTGC CAGGGTTCCC AGGTGCCCGG GGGAAGCCAG GGCCTATGGG CAAAGCTGGG 
GACAAAGGCT CACTTGGGCT TCCTGGACCT CCTGGTCCTG AGGGTTTCCC AGGAGACATT 
GGCCCCCCAG GGGACAATGG CCCTGAAGGC ATGAAGGGTA AGCCTGGAGC CCGAGGCCTA 
CCTGGACCTC CTGGGCAGCT GGGGCCTGAG GGAGATGAAG GACCCATGGG GCCACCAGGA 
GTCCCAGGCT TGGAGGGTCA ACCTGGAAGG AAGGGGTTCC CGGGGAGGCC TGGCCTGGAC 
GGCTCGAAGG GGGAACCAGG GGATCCTGGA CGGCCAGGGC CTGTGGGTGA ACAGGGGCTT 
ATGGGATTTG TTGGTCTGGT TGGAGAGCCT GGAATTGTTG GGGAGAAGGG AGACCGTGGA 
GTGATGGGAC CCCCAGGGGC ACCTGGACCC AAGGGGTCGA TGGGCCATCC TGGAACACCT 
GGTGGTGTTG GGGACCCTGG AGAGCCTGGA CCCTGGGGCC CTCCAGGATC TCGAGGACTG 
CCAGGCATGA GGGGAGCCAA GGGGCACCGG GGCCCTCGAG GACCTGATGG ACCAGCTGGG 
GAGCAGGGGT CCAAGGGCCT GAAGGGTCGT GTAGGGCCTC GGGGCAGACC TGGCCAGCCA 
GGACAGCAGG GTGCAGCTGG CGAGCGAGGC CACTCAGGTG CAAAAGGCTT CCTTGGCATT 
CCTGGTCCCT CAGGCCCTCC AGGTGCCAAG GGCCTCCCAG GAGAACCGGG CTCTCAGGGA 
CCTCAGGGGC CAGTTGGTCC TCCAGGAGAG ATGGGACCCA AGGGGCCGCC TGGTGCAGTG 
GGAGAACCTG GCCTTCCTGG GGACTCCGGT ATGAAGGGTG ACCTTGGACC TCTGGGCCCT 
CCTGGGGAAC AAGGGCTCAT TGGGCAACGG GGAGAGCCAG GCCTCGAGGG AGACCTTGGC 
CCAGTGGGGC CAGATGGGCT GAAGGGGGAC AGGGGTGACC CAGGTCCTGA TGGTGAACAT 
GGTGAAAAGG GTCAGGAAGG ACTGAAAGGA GAGGAGGGGC TCCCTGGGCC CCCAGGCATC 
ACTGGTGTCC GGGGTCGTGA AGGGAAGCCT GGCAGTCAGG GTGAGAAGGG CCAGAGGGGA 
GCTAAGGGTG CCAAGGGCTA CCAAGGACAG CTGGGAGAGA TGGGCATCCC TGGAGACCCT 
GGCCCCCCTG GCACCCCAGG TCCTAAAGGG TCCCGGGGCA CCCTAGGACC AATGGGTGCT 
CCAGGACGGA TGGGAGCCCA AGGAGAACCA GGATTGGCTG GTTATAATGG GCATAAAGGC 
ATCACGGGAC CTCTCGGGCC TCCCGGGCCC AAAGGCGAGA AGGGGGAACA GGGCGAGGAT 
GGCAAGACTG AGGGGGCCCC CGGGCCACCA GGAGAGCGGG GTCCTGTGGG TGATCGAGGA 
GACCGTGGGG AGCCAGGCGA CCCTGGATAC CCTGGTCAAG AGGGTGTTCA AGGCCTCCGT 
GGAGAACCAG GCCAGCAGGG CCAGCCCGGG CATCCAGGAC CCCGGGGACG CCCAGGACCC 
AAAGGATCAA AAGGCGAAGA GGGCCCAAAG GGAAAGCCAG GCAAGGCGGG GGCATCAGGC 
CGGAGGGGGA CCCAGGGGCT TCAAGGGCTG CCAGGGCCGA GAGGTGTAGT AGGGAGACAG 
GGCCCTGAGG GCATGGCTGG ACAAGATGGG AATCCTGGCA GGGACGGTCG GCCAGGATAT 
CAGGGAGAGC AGGGAAATGA TGGGGACCCT GGCCCCGTGG GCCCTGCTGG GAGAAGAGGG 
AATCCAGGTG TGGCTGGCTT GCCTGGAGCA CAGGGGCCTC CAGGATTCAA GGGTGAAAGT 
GGGTTACCTG GGCAACTGGG TCCCCCTGGA AAACGAGGGA CAGAAGGTGG AACGGGGCTT 
CCTGGGAACC AGGGGGAGCC AGGATCCAAA GGCCAGCCGG GTGACTCTGG CGAGATGGGC 
TTCCCAGGAG TGGCTGGCCT CTTTGGACCC AAGGGTCCCC CTGGAGACAT TGGCTTCAAA 
GGCATCCAAG GCCCTCGGGG TCCTCCTGGC TTGATGGGAA AGGAAGGTAT CATTGGGCCC 
CCCGGAATGC TGGGACCTTC TGGACTCCCG GGTCCCAAAG GTGACAGAGG CAGCCGAGGG 
GACTGGGGAC TGCAAGGCCC AAGGGGTCCT CCTGGTCCGA GGGGGCGGCC AGGTCCACCG 
GGCCCTCCTT GGCATCCTGT CCAGTTTCAA CAAGATGACC TTGAGGCAGC TTTCCAAACG 
TGGATGGATG CTCACGGAGC AGTCAGATTG GAGCAGGGGT ACAGCTATCC GGACCAGCTG 
ATGCTAGACC AGGGAGGGGA GATCTTCAAA ACCTTACACT ACCTCAGCAA CCTCATCCAG 
AGCATTAAGA CGCCCTTGGG CACCAAGGAG AACCCAGCCC GGGTCTGCCG GGACCTCATG 
GACTGCGAAC AGAAGATGGC GGATGGTATC TACTGGGTGG ACCCCAACCT CGGCTGCTCC 
TCTGATACCA TCGAAGTCTC CTGCAACTTT ACACATGGTG GGCAGACTTG CCTGAAGCCT 
ATCACGGCCT CCAAGGCAGA GTTTGCTGTT AGTCGGGTCC AGATGAATTT CTTGCACCTG 
CTCAGCTCTG AGGGGACACA GCACATCACA ATCCACTGCC TGAACATGAC GGTGTGGCAG 
GAGGGACCTG CACGCCCCTC TGCCAGGCAA GCTGTGCGCT TCCGTGCCTG GAACGGACAG 
GTCTTTGAAG CTGGGGGTCA ATTCAGGCCA GAGGTGTCTA TGGATGGCTG CAAGGTCCAT 
GATGGCCGCT GGCATCAGAC ACTATTCACC TTCCGGACCC AGGACCCCCA GCAGCTGCCC 
ATTGTCAGTG TGGACAACCT TCCACCTGTC TCGTCAGGGA AGCAGTACCG CCTGGAAGTT 
GGACCTGCAT GCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Collagen XXVII organises the pericellular matrix in the growth plate.
PloS one6(12)e29422(2011)
Plumb DA,Ferrara L,Torbica T,Knowles L,Mironov A Jr,Kadler KE,Briggs MD,Boot-Handford RP

Collagen XXVII is developmentally regulated and forms thin fibrillar structures distinct from those of classical vertebrate fibrillar collagens.
The Journal of biological chemistry282(17)12791-5(2007 Apr)
Plumb DA,Dhir V,Mironov A,Ferrara L,Poulsom R,Kadler KE,Thornton DJ,Briggs MD,Boot-Handford RP

Identification, characterization and expression analysis of a new fibrillar collagen gene, COL27A1.
Matrix biology : journal of the International Society for Matrix Biology22(1)3-14(2003 Mar)
Pace JM,Corrado M,Missero C,Byers PH

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Col27a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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