parp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	parp1
Entrez Gene ID	100490557
Full Name	poly(ADP-ribose) polymerase 1
Synonyms	adprt,adprt1,padprt-1,parp,parp-1,ppol
General protein information	Preferred Names poly(ADP-ribose) polymerase 1 Names poly [ADP-ribose] polymerase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002941128.4	XP_002941174.2	poly [ADP-ribose] polymerase 1
XM_031902056.1	XP_031757916.1	poly [ADP-ribose] polymerase 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PARP1	NP_850165.1
Oryza sativa (rice)	Os07g0413700	NP_001059453.1
Drosophila melanogaster (fruit fly)	Parp	NP_001104452.1
Homo sapiens (human)	PARP1	NP_001609.2
Pan troglodytes (chimpanzee)	PARP1	XP_003949754.1
Canis lupus familiaris (dog)	PARP1	XP_547506.3
Bos taurus (cattle)	PARP1	NP_777176.1
Macaca mulatta (Rhesus monkey)	PARP1	XP_001090984.1
Gallus gallus (chicken)	PARP1	NP_990594.1
Xenopus tropicalis (tropical clawed frog)	parp1	XP_002941174.2
Danio rerio (zebrafish)	parp1	NP_001038407.1
Caenorhabditis elegans (roundworm)	pme-1	NP_491072.1
Mus musculus (house mouse)	Parp1	NP_031441.2
Rattus norvegicus (Norway rat)	Parp1	NP_037195.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003230	XP_312938.4

Gene Ontology

The following parp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the parp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa10960	XM_002941128.4 Latest version!	Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa58355	XM_031902056.1 Latest version!	Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

parp1 ( XM_002941128.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941174.2 Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.

Price & Availability↑

CloneID	OXa10960
Clone ID Related Accession (Same CDS sequence)	XM_002941128.4
Accession Version	XM_002941128.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	poly [ADP-ribose] polymerase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCTCGGAGAA ACTTTATCGG GCTGAGTACG CCAAGAGCGG CCGCGCCTCC 
TGTAAGAAAT GCGGAGACAA CATCGCCAAG GAGTCCCTGC GGCTTGCCCT CATGGTGCAG 
TCCCCAATGT TCGATGGCAA AGTGCCACAC TGGCACCACT ACTCCTGTTT CTGGAAACGC 
GCCCGGGTCT TATCCCATGG GGATATCGAT GGTTTCACTG AGCTCCGATG GGACGATCAG 
GAAAAAATCA AGAAAGCCGT TGAAACCGGA GGGGCTTCTG CAGGGGCCGG AGGGGATGCC 
AAGGGAGGCA AAGGGGAGAT GACGCTGAAT GATTTTGCAG TAGAATATGC AAAGTCTAAC 
AGAAGCACCT GCAAAGGCTG TGAGAAGAAA ATAGAAAAGG GGCAGATCAG AATATCCAAG 
AAGATGGTGG ATGTGGAGCG GCCCCAGCTG GGAATGATCG ACCGGTGGTA TCACTCAGAC 
TGCTTTGTGA GCTGCAGGGA GGAGCTGGGC TTTCTCCCCT CCTACAGCGC CAGCCAGCTC 
AAGGCTTTCT CCATTCTAAA AGCTGAGGAC AAAGATGCCC TGAAGGCAAT GCTTCCCGCT 
GTAAAGAATG AAGGGAAGAG GAAGGCAGAT GAGGTGGATG CAAGTTCCGC TGGAACCAAA 
AAGAAATTAA AGAAAGAGAA GGAAAAAGAG TCAAAGCTGG AAAAGTTGCT AAAGGAGCAG 
ACAGAGCTTA TTTGGCACAT TAAGGATGAG CTGAAAAAAG TTTGCAGCAC CAACGATTTG 
AAGGAGCTTC TCATCGCCAA CAAACAGCAA GTGCCGTCCG GTGAGACCAA CATTTTGGAC 
CGGGTATCAG ATGGCATGGC ATTTGGAGCA CTGCTTCCGT GCGAGGAGTG CAGCGGGCAG 
TTTGTTTTCA AGAGCGACGC TTATTATTGC ACCGGGGACC TCAGCGCCTG GACAAAATGT 
GTTGCTAAAA CACAGACTCC CAACAGAAAG GATTGGGTGA CACCTAAGGA GTTCCATGAA 
GTCCCTTATT TGAAGAAATT TAAGTTCAAA AGACAAGACA GAGCTTTCCC GCCTTGTGCC 
GCTCCAACAC CTATCAGCCC CCCGGCTGCC CCTAAACCAG CGCCGACTGT GGAGGAAACT 
GTTCCTGAAG GGAAACCTTT AACCAATGCA AAGGTGCTTG TCATTGGGAA GCTGTCCAAA 
AACAAGGATG AGGTAAAGGC GTTGATTGAA GGACTGGGCG GCAAAGTCAC CGGCTCTGCA 
CACAAGGCCA CGCTGTGCAT CAGCACTAAA AAGGAAGTTG AGAAGATGAG TAAGAAGATG 
GAGGATGTGA AAGCAGCCAA TGTGCGTGTA GTAAGTGACG ACTTCCTGAA GGAAGTGGAG 
TCGGGGAAGA GCGTGCAGGA ATTGTTGTCC CAATACGGAA TATCCTCATG GGGTGCTGAG 
GTCAAGCAAG AGGCCGTGCA GCCAACGGAG AAGCAGCCGT CCAGCGCCCC CGCGGCTGGG 
AAGAGTTCGG GCAAAGTGAA AGAAGAGAAA GGGAGCAACA AGTCTGAAAA GAAAATGAAA 
TTAACTGTTA AAGGGGGAGC AGCCATTGAC CCCGATTCTG GGCTGGAAGA TTCATGTCAC 
GTCTTGGAAA AAGGAGGGAA GATTTTTAGT GCAACTCTGG GCCTGGTGGA TATAACCAGA 
GGGACAAATT CTTATTATAA GCTGCAACTT ATAGAGCACG ACAGAAATTC CAGGTACTGG 
GTGTTCCGAT CCTGGGGTCG AGTGGGCACT GTAATAGGCA GTAACAAGCT AGAAGAGATG 
TCTTCCAAGG AAGATGCCAT TGACCATTTC CTCAACCTGT ATCAGGAGAA AACCGGCAAT 
GCATGGCACT CCCCCAATTT CACCAAATAT CCCAACAAGT TCTATCCTCT GGAAATAGAC 
TATGGGCAGG AAGAAGATGT GGTGAAGAAA CTCTCGGTTG GGGCCGGCAC AAAATCCAAG 
CTTGCCCAGC CTGTCCAGGA ACTGATCAAG TTAATATTTG ATGTGGAGAG CATGAAGAAG 
GCCATGGTGG AGTTTGAGAT CGATCTCCAG AAGATGCCTT TGGGGAAGCT CAGCAAGCGG 
CAGATTCAGA GTGCATACTC AATACTCAAT CAAGTGCAGC AGGCCGTCTC GGAATCCTCA 
AGCGAAGCTC GGTTATTGGA TCTCTCCAAT CAGTTTTACA CACTCATACC TCATGATTTT 
GGAATGAAGA AGCCCCCGCT GCTAAATAAT CTAGAGTACA TACAGGCTAA AGTGCAGATG 
CTGGATAATC TGCTTGACAT TGAAGTTGCA TACAGCCTGT TGAGAGGCGG CGCCGATGAT 
GGAGAAAAAG ATCCCATTGA TGTGAAATAT GAAAAGATTA AGACTGACAT TAAGGTTGTC 
GATAAAGAAT CGGAAGAATC TAGAATAATA CGTGACTATG TGAAGAACAC GCACGCCGAG 
ACGCACAATG CCTACGATCT TGAGGTCCTC GAGATATTCA AAATCGAGCG TGAAGGTGAA 
TATCAGCGGT ATAAGCCATT CAAACAGCTA CACAACCGCC AGCTGCTTTG GCACGGATCC 
CGAACCACAA ATTTTGCAGG GATATTGTCT CAGGGTCTCC GAATTGCTCC ACCGGAAGCT 
CCCGTTACTG GCTATATGTT TGGCAAAGGT ATCTATTTTG CCGATATGGT ATCCAAAAGT 
GCAAACTATT GTAATGCAAT GCCGGGTAAT CCCATTGGGC TGATCTTACT GGGAGAGGTG 
GCCCTTGGTA ACATGCATGA GCTGAGAGCA GCATCACACA TTACGAAACT ACCCAAGGGC 
AAACACAGTG TCAAAGGTTT GGGTCGAACC GCGCCTGATC CCTCAGCTAC TGTAAAACTG 
GATGGAGTGG ACGTTCCTCT TGGGAAAGGA AGTGCTACAA ATATCACTGA CGCCAGTCTG 
TTATATAACG AATACATTGT GTATGATGTT GCTCAGGTTA ACCTGAAGTA CCTGCTGAAG 
CTGAAGTTCA ACTACAAAGA CAAAGGAGGG TTGCTTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941174.2
CDS	129..3170
Translation	MAEASEKLYRAEYAKSGRASCKKCGDNIAKESLRLALMVQSPMFDGKVPHWHHYSCFWKRARVLSHGDIDGFTELRWDDQEKIKKAVETGGASAGAGGDAKGGKGEMTLNDFAVEYAKSNRSTCKGCEKKIEKGQIRISKKMVDVERPQLGMIDRWYHSDCFVSCREELGFLPSYSASQLKAFSILKAEDKDALKAMLPAVKNEGKRKADEVDASSAGTKKKLKKEKEKESKLEKLLKEQTELIWHIKDELKKVCSTNDLKELLIANKQQVPSGETNILDRVSDGMAFGALLPCEECSGQFVFKSDAYYCTGDLSAWTKCVAKTQTPNRKDWVTPKEFHEVPYLKKFKFKRQDRAFPPCAAPTPISPPAAPKPAPTVEETVPEGKPLTNAKVLVIGKLSKNKDEVKALIEGLGGKVTGSAHKATLCISTKKEVEKMSKKMEDVKAANVRVVSDDFLKEVESGKSVQELLSQYGISSWGAEVKQEAVQPTEKQPSSAPAAGKSSGKVKEEKGSNKSEKKMKLTVKGGAAIDPDSGLEDSCHVLEKGGKIFSATLGLVDITRGTNSYYKLQLIEHDRNSRYWVFRSWGRVGTVIGSNKLEEMSSKEDAIDHFLNLYQEKTGNAWHSPNFTKYPNKFYPLEIDYGQEEDVVKKLSVGAGTKSKLAQPVQELIKLIFDVESMKKAMVEFEIDLQKMPLGKLSKRQIQSAYSILNQVQQAVSESSSEARLLDLSNQFYTLIPHDFGMKKPPLLNNLEYIQAKVQMLDNLLDIEVAYSLLRGGADDGEKDPIDVKYEKIKTDIKVVDKESEESRIIRDYVKNTHAETHNAYDLEVLEIFKIEREGEYQRYKPFKQLHNRQLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCNAMPGNPIGLILLGEVALGNMHELRAASHITKLPKGKHSVKGLGRTAPDPSATVKLDGVDVPLGKGSATNITDASLLYNEYIVYDVAQVNLKYLLKLKFNYKDKGGLLW

Target ORF information:

RefSeq Version	XM_002941128.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941128.4

ORF Insert Sequence:

ATGGCCGAGG CCTCGGAGAA ACTTTATCGG GCTGAGTACG CCAAGAGCGG CCGCGCCTCC 
TGTAAGAAAT GCGGAGACAA CATCGCCAAG GAGTCCCTGC GGCTTGCCCT CATGGTGCAG 
TCCCCAATGT TCGATGGCAA AGTGCCACAC TGGCACCACT ACTCCTGTTT CTGGAAACGC 
GCCCGGGTCT TATCCCATGG GGATATCGAT GGTTTCACTG AGCTCCGATG GGACGATCAG 
GAAAAAATCA AGAAAGCCGT TGAAACCGGA GGGGCTTCTG CAGGGGCCGG AGGGGATGCC 
AAGGGAGGCA AAGGGGAGAT GACGCTGAAT GATTTTGCAG TAGAATATGC AAAGTCTAAC 
AGAAGCACCT GCAAAGGCTG TGAGAAGAAA ATAGAAAAGG GGCAGATCAG AATATCCAAG 
AAGATGGTGG ATGTGGAGCG GCCCCAGCTG GGAATGATCG ACCGGTGGTA TCACTCAGAC 
TGCTTTGTGA GCTGCAGGGA GGAGCTGGGC TTTCTCCCCT CCTACAGCGC CAGCCAGCTC 
AAGGCTTTCT CCATTCTAAA AGCTGAGGAC AAAGATGCCC TGAAGGCAAT GCTTCCCGCT 
GTAAAGAATG AAGGGAAGAG GAAGGCAGAT GAGGTGGATG CAAGTTCCGC TGGAACCAAA 
AAGAAATTAA AGAAAGAGAA GGAAAAAGAG TCAAAGCTGG AAAAGTTGCT AAAGGAGCAG 
ACAGAGCTTA TTTGGCACAT TAAGGATGAG CTGAAAAAAG TTTGCAGCAC CAACGATTTG 
AAGGAGCTTC TCATCGCCAA CAAACAGCAA GTGCCGTCCG GTGAGACCAA CATTTTGGAC 
CGGGTATCAG ATGGCATGGC ATTTGGAGCA CTGCTTCCGT GCGAGGAGTG CAGCGGGCAG 
TTTGTTTTCA AGAGCGACGC TTATTATTGC ACCGGGGACC TCAGCGCCTG GACAAAATGT 
GTTGCTAAAA CACAGACTCC CAACAGAAAG GATTGGGTGA CACCTAAGGA GTTCCATGAA 
GTCCCTTATT TGAAGAAATT TAAGTTCAAA AGACAAGACA GAGCTTTCCC GCCTTGTGCC 
GCTCCAACAC CTATCAGCCC CCCGGCTGCC CCTAAACCAG CGCCGACTGT GGAGGAAACT 
GTTCCTGAAG GGAAACCTTT AACCAATGCA AAGGTGCTTG TCATTGGGAA GCTGTCCAAA 
AACAAGGATG AGGTAAAGGC GTTGATTGAA GGACTGGGCG GCAAAGTCAC CGGCTCTGCA 
CACAAGGCCA CGCTGTGCAT CAGCACTAAA AAGGAAGTTG AGAAGATGAG TAAGAAGATG 
GAGGATGTGA AAGCAGCCAA TGTGCGTGTA GTAAGTGACG ACTTCCTGAA GGAAGTGGAG 
TCGGGGAAGA GCGTGCAGGA ATTGTTGTCC CAATACGGAA TATCCTCATG GGGTGCTGAG 
GTCAAGCAAG AGGCCGTGCA GCCAACGGAG AAGCAGCCGT CCAGCGCCCC CGCGGCTGGG 
AAGAGTTCGG GCAAAGTGAA AGAAGAGAAA GGGAGCAACA AGTCTGAAAA GAAAATGAAA 
TTAACTGTTA AAGGGGGAGC AGCCATTGAC CCCGATTCTG GGCTGGAAGA TTCATGTCAC 
GTCTTGGAAA AAGGAGGGAA GATTTTTAGT GCAACTCTGG GCCTGGTGGA TATAACCAGA 
GGGACAAATT CTTATTATAA GCTGCAACTT ATAGAGCACG ACAGAAATTC CAGGTACTGG 
GTGTTCCGAT CCTGGGGTCG AGTGGGCACT GTAATAGGCA GTAACAAGCT AGAAGAGATG 
TCTTCCAAGG AAGATGCCAT TGACCATTTC CTCAACCTGT ATCAGGAGAA AACCGGCAAT 
GCATGGCACT CCCCCAATTT CACCAAATAT CCCAACAAGT TCTATCCTCT GGAAATAGAC 
TATGGGCAGG AAGAAGATGT GGTGAAGAAA CTCTCGGTTG GGGCCGGCAC AAAATCCAAG 
CTTGCCCAGC CTGTCCAGGA ACTGATCAAG TTAATATTTG ATGTGGAGAG CATGAAGAAG 
GCCATGGTGG AGTTTGAGAT CGATCTCCAG AAGATGCCTT TGGGGAAGCT CAGCAAGCGG 
CAGATTCAGA GTGCATACTC AATACTCAAT CAAGTGCAGC AGGCCGTCTC GGAATCCTCA 
AGCGAAGCTC GGTTATTGGA TCTCTCCAAT CAGTTTTACA CACTCATACC TCATGATTTT 
GGAATGAAGA AGCCCCCGCT GCTAAATAAT CTAGAGTACA TACAGGCTAA AGTGCAGATG 
CTGGATAATC TGCTTGACAT TGAAGTTGCA TACAGCCTGT TGAGAGGCGG CGCCGATGAT 
GGAGAAAAAG ATCCCATTGA TGTGAAATAT GAAAAGATTA AGACTGACAT TAAGGTTGTC 
GATAAAGAAT CGGAAGAATC TAGAATAATA CGTGACTATG TGAAGAACAC GCACGCCGAG 
ACGCACAATG CCTACGATCT TGAGGTCCTC GAGATATTCA AAATCGAGCG TGAAGGTGAA 
TATCAGCGGT ATAAGCCATT CAAACAGCTA CACAACCGCC AGCTGCTTTG GCACGGATCC 
CGAACCACAA ATTTTGCAGG GATATTGTCT CAGGGTCTCC GAATTGCTCC ACCGGAAGCT 
CCCGTTACTG GCTATATGTT TGGCAAAGGT ATCTATTTTG CCGATATGGT ATCCAAAAGT 
GCAAACTATT GTAATGCAAT GCCGGGTAAT CCCATTGGGC TGATCTTACT GGGAGAGGTG 
GCCCTTGGTA ACATGCATGA GCTGAGAGCA GCATCACACA TTACGAAACT ACCCAAGGGC 
AAACACAGTG TCAAAGGTTT GGGTCGAACC GCGCCTGATC CCTCAGCTAC TGTAAAACTG 
GATGGAGTGG ACGTTCCTCT TGGGAAAGGA AGTGCTACAA ATATCACTGA CGCCAGTCTG 
TTATATAACG AATACATTGT GTATGATGTT GCTCAGGTTA ACCTGAAGTA CCTGCTGAAG 
CTGAAGTTCA ACTACAAAGA CAAAGGAGGG TTGCTTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

parp1 ( XM_031902056.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757916.1 Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.

Price & Availability↑

CloneID	OXa58355
Clone ID Related Accession (Same CDS sequence)	XM_031902056.1
Accession Version	XM_031902056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	poly [ADP-ribose] polymerase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCTCGGAGAA ACTTTATCGG GCTGAGTACG CCAAGAGCGG CCGCGCCTCC 
TGTAAGAAAT GCGGAGACAA CATCGCCAAG GAGTCCCTGC GGCTTGCCCT CATGGTGCAG 
TCCCCAATGT TCGATGGCAA AGTGCCACAC TGGCACCACT ACTCCTGTTT CTGGAAACGC 
GCCCGGGTCT TATCCCATGG GGATATTGAT GGTTTCACTG AGCTCCGATG GGACGATCAG 
GAAAAAATCA AGAAAGCCAT TGAAACCGGA GGGGCTTCTG CAGGGGCCGG AGGGGATGCC 
AAGGGAGGCA AAGGGGAGAT GACGCTGAAT GATTTTGCAG TAGAATATGC AAAGTCTAAC 
AGAAGCACCT GCAAAGGCTG TGAGAAGAAA ATAGAAAAGG GGCAGATCAG AATATCCAAG 
AAGATGGTGG ATGTGGAGCG GCCCCAGCTG GGAATGATCG ACCGGTGGTA TCACTCAGAC 
TGCTTTGTGA GCTGCAGGGA GGAGCTGGGC TTTCTCCCCT CCTACAGCGC CAGCCAGCTC 
AAGGCTTTCT CCATTCTAAA AGCTGAGGAC AAAGATGCCC TGAAGGCAAT GCTTCCCGCT 
GTAAAGAATG AAGGGAAGAG GAAGGCAGAT GAGGTGGATG CAAGTTCCGC TGGAACCAAA 
AAGAAATTAA AGAAAGAGAA GGAAAAAGAG TCAAAGCTGG AAAAGTTGCT AAAGGAGCAG 
ACAGAGCTTA TTTGGCACAT TAAGGATGAG CTGAAAAAAG TTTGCAGCAC CAACGATTTG 
AAGGAGCTTC TCATCGCCAA CAAACAGCAA GTGCCGTCCG GTGAGACCAA CATTTTGGAC 
CGGGTATCAG ATGGCATGGC ATTTGGAGCA CTGCTTCCGT GCGAGGAGTG CAGCGGGCAG 
TTTGTTTTCA AGAGCGACGC TTATTATTGC ACCGGGGACC TCAGCGCCTG GACAAAATGT 
GTTGCTAAAA CACAGACTCC CAACAGAAAG GATTGGGTGA CACCTAAGGA GTTCCATGAA 
GTCCCTTATT TGAAGAAATT TAAGTTCAAA AGACAAGACA GAGCTTTCCC GCCTTGTGCC 
GCTCCAACAC CTATCAGCCC CCCGGCTGCC CCTAAACCAG CGCCGACTGT GGAGGAAACT 
GTTCCTGAAG GGAAACCTTT AACCAATGCA AAGGTGCTTG TCATTGGGAA GCTGTCCAAA 
AACAAGGATG AGGTAAAGGC GTTGATTGAA GGACTGGGCG GCAAAGTCAC CGGCTCTGCA 
CACAAGGCCA CGCTGTGCAT CAGCACTAAA AAGGAAGTTG AGAAGATGAG TAAGAAGATG 
GAGGATGTGA AAGCAGCCAA TGTGCGTGTA GTAAGTGACG ACTTCCTGAA GGAAGTGGAG 
TCGGGGAAGA GCGTGCAGGA ATTGTTGTCC CAATACGGAA TATCCTCATG GGGTGCTGAG 
GTCAAGCAAG AGGCCGTGCA GCCAACGGAG AAGCAGCCGT CCAGCGCCCC CGCGGCTGGG 
AAGAGTTCGG GCAAAGTGAA AGAAGAGAAA GGGAGCAACA AGTCTGAAAA GAAAATGAAA 
TTAACTGTTA AAGGGGGAGC AGCCATTGAC CCCGATTCTG GGCTGGAAGA TTCATGTCAC 
GTCTTGGAAA AAGGAGGGAA GATTTTTAGT GCAACTCTGG GCCTGGTGGA TATAACCAGA 
GGGACAAATT CTTATTATAA GCTGCAACTT ATAGAGCACG ACAGAAATTC CAGGTACTGG 
GTGTTCCGAT CCTGGGGTCG AGTGGGCACT GTAATAGGCA GTAACAAGCT AGAAGAGATG 
TCTTCCAAGG AAGATGCCAT TGACCATTTC CTCAACCTGT ATCAGGAGAA AACCGGCAAT 
GCATGGCACT CCCCCAATTT CACCAAATAT CCCAACAAGT TCTATCCTCT GGAAATAGAC 
TATGGGCAGG AAGAAGATGT GGTGAAGAAA CTCTCGGTTG GGGCCGGCAC AAAATCCAAG 
CTTGCCCAGC CTGTCCAGGA ACTGATCAAG TTAATATTTG ATGTGGAGAG CATGAAGAAG 
GCCATGGTGG AGTTTGAGAT CGATCTCCAG AAGATGCCTT TGGGGAAGCT CAGCAAGCGG 
CAGATTCAGA GTGCATACTC AATACTCAAT CAAGTGCAGC AGGCCGTCTC GGAATCCTCA 
AGCGAAGCTC GGTTATTGGA TCTCTCCAAT CAGTTTTACA CACTCATACC TCATGATTTT 
GGAATGAAGA AGCCCCCGCT GCTAAATAAT CTAGAGTACA TACAGGCTAA AGTGCAGATG 
CTGGATAATC TGCTTGACAT TGAAGTTGCA TACAGCCTGT TGAGAGGCGG CGCCGATGAT 
GGAGAAAAAG ATCCCATTGA TGTGAAATAT GAAAAGATTA AGACTGACAT TAAGGTTGTC 
GATAAAGAAT CGGAAGAATC TAGAATAATA CGTGACTATG TGAAGAACAC GCACGCCGAG 
ACGCACAATG CCTACGATCT TGAGGTCCTC GAGATATTCA AAATCGAGCG TGAAGGTGAA 
TATCAGCGGT ATAAGCCATT CAAACAGCTA CACAACCGCC AGCTGCTTTG GCACGGATCC 
CGAACCACAA ATTTTGCAGG GATATTGTCT CAGGGTCTCC GAATTGCTCC ACCGGAAGCT 
CCCGTTACTG GCTATATGTT TGGCAAAGGT ATCTATTTTG CCGATATGGT ATCCAAAAGT 
GCCAACTATT GTAATGCAAT GCCGGGTAAT CCCATTGGGC TGATCTTACT GGGAGAGGTG 
GCCCTTGGTA ACATGCATGA GCTGAGAGCA GCATCACACA TTACGAAACT ACCCAAGGGC 
AAACACAGTG TCAAAGGTTT GGGTAGAACC GCGCCTGATC CCTCAGCTAC TGTAAAACTG 
GATGGAGTGG ACGTTCCTCT TGGGAAAGGA AGTGCTACAA ATATCACTGA CGCCAGTCTG 
TTATATAACG AATACATTGT GTATGATGTT GCTCAGGTTA ACCTGAAGTA CCTGCTGAAG 
CTGAAGTTCA ACTACAAAGA CAAAGGAGGG TTGCTTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757916.1
CDS	115..3156
Translation	MAEASEKLYRAEYAKSGRASCKKCGDNIAKESLRLALMVQSPMFDGKVPHWHHYSCFWKRARVLSHGDIDGFTELRWDDQEKIKKAIETGGASAGAGGDAKGGKGEMTLNDFAVEYAKSNRSTCKGCEKKIEKGQIRISKKMVDVERPQLGMIDRWYHSDCFVSCREELGFLPSYSASQLKAFSILKAEDKDALKAMLPAVKNEGKRKADEVDASSAGTKKKLKKEKEKESKLEKLLKEQTELIWHIKDELKKVCSTNDLKELLIANKQQVPSGETNILDRVSDGMAFGALLPCEECSGQFVFKSDAYYCTGDLSAWTKCVAKTQTPNRKDWVTPKEFHEVPYLKKFKFKRQDRAFPPCAAPTPISPPAAPKPAPTVEETVPEGKPLTNAKVLVIGKLSKNKDEVKALIEGLGGKVTGSAHKATLCISTKKEVEKMSKKMEDVKAANVRVVSDDFLKEVESGKSVQELLSQYGISSWGAEVKQEAVQPTEKQPSSAPAAGKSSGKVKEEKGSNKSEKKMKLTVKGGAAIDPDSGLEDSCHVLEKGGKIFSATLGLVDITRGTNSYYKLQLIEHDRNSRYWVFRSWGRVGTVIGSNKLEEMSSKEDAIDHFLNLYQEKTGNAWHSPNFTKYPNKFYPLEIDYGQEEDVVKKLSVGAGTKSKLAQPVQELIKLIFDVESMKKAMVEFEIDLQKMPLGKLSKRQIQSAYSILNQVQQAVSESSSEARLLDLSNQFYTLIPHDFGMKKPPLLNNLEYIQAKVQMLDNLLDIEVAYSLLRGGADDGEKDPIDVKYEKIKTDIKVVDKESEESRIIRDYVKNTHAETHNAYDLEVLEIFKIEREGEYQRYKPFKQLHNRQLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCNAMPGNPIGLILLGEVALGNMHELRAASHITKLPKGKHSVKGLGRTAPDPSATVKLDGVDVPLGKGSATNITDASLLYNEYIVYDVAQVNLKYLLKLKFNYKDKGGLLW

Target ORF information:

RefSeq Version	XM_031902056.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis poly(ADP-ribose) polymerase 1 (parp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902056.1

ORF Insert Sequence:

ATGGCCGAGG CCTCGGAGAA ACTTTATCGG GCTGAGTACG CCAAGAGCGG CCGCGCCTCC 
TGTAAGAAAT GCGGAGACAA CATCGCCAAG GAGTCCCTGC GGCTTGCCCT CATGGTGCAG 
TCCCCAATGT TCGATGGCAA AGTGCCACAC TGGCACCACT ACTCCTGTTT CTGGAAACGC 
GCCCGGGTCT TATCCCATGG GGATATTGAT GGTTTCACTG AGCTCCGATG GGACGATCAG 
GAAAAAATCA AGAAAGCCAT TGAAACCGGA GGGGCTTCTG CAGGGGCCGG AGGGGATGCC 
AAGGGAGGCA AAGGGGAGAT GACGCTGAAT GATTTTGCAG TAGAATATGC AAAGTCTAAC 
AGAAGCACCT GCAAAGGCTG TGAGAAGAAA ATAGAAAAGG GGCAGATCAG AATATCCAAG 
AAGATGGTGG ATGTGGAGCG GCCCCAGCTG GGAATGATCG ACCGGTGGTA TCACTCAGAC 
TGCTTTGTGA GCTGCAGGGA GGAGCTGGGC TTTCTCCCCT CCTACAGCGC CAGCCAGCTC 
AAGGCTTTCT CCATTCTAAA AGCTGAGGAC AAAGATGCCC TGAAGGCAAT GCTTCCCGCT 
GTAAAGAATG AAGGGAAGAG GAAGGCAGAT GAGGTGGATG CAAGTTCCGC TGGAACCAAA 
AAGAAATTAA AGAAAGAGAA GGAAAAAGAG TCAAAGCTGG AAAAGTTGCT AAAGGAGCAG 
ACAGAGCTTA TTTGGCACAT TAAGGATGAG CTGAAAAAAG TTTGCAGCAC CAACGATTTG 
AAGGAGCTTC TCATCGCCAA CAAACAGCAA GTGCCGTCCG GTGAGACCAA CATTTTGGAC 
CGGGTATCAG ATGGCATGGC ATTTGGAGCA CTGCTTCCGT GCGAGGAGTG CAGCGGGCAG 
TTTGTTTTCA AGAGCGACGC TTATTATTGC ACCGGGGACC TCAGCGCCTG GACAAAATGT 
GTTGCTAAAA CACAGACTCC CAACAGAAAG GATTGGGTGA CACCTAAGGA GTTCCATGAA 
GTCCCTTATT TGAAGAAATT TAAGTTCAAA AGACAAGACA GAGCTTTCCC GCCTTGTGCC 
GCTCCAACAC CTATCAGCCC CCCGGCTGCC CCTAAACCAG CGCCGACTGT GGAGGAAACT 
GTTCCTGAAG GGAAACCTTT AACCAATGCA AAGGTGCTTG TCATTGGGAA GCTGTCCAAA 
AACAAGGATG AGGTAAAGGC GTTGATTGAA GGACTGGGCG GCAAAGTCAC CGGCTCTGCA 
CACAAGGCCA CGCTGTGCAT CAGCACTAAA AAGGAAGTTG AGAAGATGAG TAAGAAGATG 
GAGGATGTGA AAGCAGCCAA TGTGCGTGTA GTAAGTGACG ACTTCCTGAA GGAAGTGGAG 
TCGGGGAAGA GCGTGCAGGA ATTGTTGTCC CAATACGGAA TATCCTCATG GGGTGCTGAG 
GTCAAGCAAG AGGCCGTGCA GCCAACGGAG AAGCAGCCGT CCAGCGCCCC CGCGGCTGGG 
AAGAGTTCGG GCAAAGTGAA AGAAGAGAAA GGGAGCAACA AGTCTGAAAA GAAAATGAAA 
TTAACTGTTA AAGGGGGAGC AGCCATTGAC CCCGATTCTG GGCTGGAAGA TTCATGTCAC 
GTCTTGGAAA AAGGAGGGAA GATTTTTAGT GCAACTCTGG GCCTGGTGGA TATAACCAGA 
GGGACAAATT CTTATTATAA GCTGCAACTT ATAGAGCACG ACAGAAATTC CAGGTACTGG 
GTGTTCCGAT CCTGGGGTCG AGTGGGCACT GTAATAGGCA GTAACAAGCT AGAAGAGATG 
TCTTCCAAGG AAGATGCCAT TGACCATTTC CTCAACCTGT ATCAGGAGAA AACCGGCAAT 
GCATGGCACT CCCCCAATTT CACCAAATAT CCCAACAAGT TCTATCCTCT GGAAATAGAC 
TATGGGCAGG AAGAAGATGT GGTGAAGAAA CTCTCGGTTG GGGCCGGCAC AAAATCCAAG 
CTTGCCCAGC CTGTCCAGGA ACTGATCAAG TTAATATTTG ATGTGGAGAG CATGAAGAAG 
GCCATGGTGG AGTTTGAGAT CGATCTCCAG AAGATGCCTT TGGGGAAGCT CAGCAAGCGG 
CAGATTCAGA GTGCATACTC AATACTCAAT CAAGTGCAGC AGGCCGTCTC GGAATCCTCA 
AGCGAAGCTC GGTTATTGGA TCTCTCCAAT CAGTTTTACA CACTCATACC TCATGATTTT 
GGAATGAAGA AGCCCCCGCT GCTAAATAAT CTAGAGTACA TACAGGCTAA AGTGCAGATG 
CTGGATAATC TGCTTGACAT TGAAGTTGCA TACAGCCTGT TGAGAGGCGG CGCCGATGAT 
GGAGAAAAAG ATCCCATTGA TGTGAAATAT GAAAAGATTA AGACTGACAT TAAGGTTGTC 
GATAAAGAAT CGGAAGAATC TAGAATAATA CGTGACTATG TGAAGAACAC GCACGCCGAG 
ACGCACAATG CCTACGATCT TGAGGTCCTC GAGATATTCA AAATCGAGCG TGAAGGTGAA 
TATCAGCGGT ATAAGCCATT CAAACAGCTA CACAACCGCC AGCTGCTTTG GCACGGATCC 
CGAACCACAA ATTTTGCAGG GATATTGTCT CAGGGTCTCC GAATTGCTCC ACCGGAAGCT 
CCCGTTACTG GCTATATGTT TGGCAAAGGT ATCTATTTTG CCGATATGGT ATCCAAAAGT 
GCCAACTATT GTAATGCAAT GCCGGGTAAT CCCATTGGGC TGATCTTACT GGGAGAGGTG 
GCCCTTGGTA ACATGCATGA GCTGAGAGCA GCATCACACA TTACGAAACT ACCCAAGGGC 
AAACACAGTG TCAAAGGTTT GGGTAGAACC GCGCCTGATC CCTCAGCTAC TGTAAAACTG 
GATGGAGTGG ACGTTCCTCT TGGGAAAGGA AGTGCTACAA ATATCACTGA CGCCAGTCTG 
TTATATAACG AATACATTGT GTATGATGTT GCTCAGGTTA ACCTGAAGTA CCTGCTGAAG 
CTGAAGTTCA ACTACAAAGA CAAAGGAGGG TTGCTTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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parp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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