ngef cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ngef
Entrez Gene ID	100487663
Full Name	neuronal guanine nucleotide exchange factor
General protein information	Preferred Names neuronal guanine nucleotide exchange factor Names ephexin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933353.2	XP_002933399.1	ephexin-1 isoform X1
XM_004917796.4	XP_004917853.1	ephexin-1 isoform X2
XM_031902564.1	XP_031758424.1	ephexin-1 isoform X1
XM_002933353.3	XP_002933399.1	ephexin-1 isoform X1
XM_004917796.3	XP_004917853.1	ephexin-1 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NGEF	XP_005635921.1
Danio rerio (zebrafish)	LOC569090	XP_697546.3
Xenopus tropicalis (tropical clawed frog)	ngef	XP_004917852.1
Bos taurus (cattle)	NGEF	NP_001070394.1
Rattus norvegicus (Norway rat)	Ngef	NP_001129713.1
Pan troglodytes (chimpanzee)	NGEF	XP_516157.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007452	XP_308411.4
Homo sapiens (human)	NGEF	NP_062824.2
Macaca mulatta (Rhesus monkey)	LOC713917	XP_001114604.1
Mus musculus (house mouse)	Ngef	NP_001104784.1
Gallus gallus (chicken)	NGEF	NP_001010841.1

Gene Ontology

The following ngef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ngef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16053	XM_002933353.3 Latest version!	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa16053	XM_031902564.1 Latest version!	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa16054	XM_004917796.4 Latest version!	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa16053	XM_002933353.2	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa16054	XM_004917796.3	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ngef ( XM_002933353.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933399.1 Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16053
Clone ID Related Accession (Same CDS sequence)	XM_002933353.2 , XM_031902564.1 , XM_002933353.3
Accession Version	XM_002933353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002933353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933399.1
CDS	205..2280
Translation	MRRFMRETLTQSRDSRMGRDALTASSVINMREPQIKNEEESEDENQMDESDSQTRHMPIRRNSRFYRSMRVRNRHRRDRLSSREGMEIDNQTTAQQTYDGSRSIHLSVHYNPTTGPAGYREYLPSEDTSNPHEDSSSPSECATSNSEAPPTIYPLPSDSFKTIIDHIEFLYQEYRDKSTQHEIETRRLQDNYVQSAETSNNSQNTPLGSPPPIENQRANSTFSRFESSCSGFNLWQDLPDVRKSGVLALLQPEEVKLQEAMFELLTSEASYYKSLRLVVSHFMDSERLRASLHQSEIHFIFSNVLEVLATSERFLLALEQRFEENIVISDVCDIVYEHAEKHFVVYVTYVCNQTYQERAYRKLLQERPEFRESVGHLETDPVCRGLPFSSFLILPFQRITRLKLLVQNILTKVEEGSEREVSALQAHRELEKLVRACNDGVRKMSRTEQLISIEKTLEFKIKSVPIISHSRWLMKQGELQQMSGPRTSRTLRTKKLFRPVYLFLFNDLLLMCKPLTGERYQVFDVAARGLLRVEELEDQGQTLANVFILKLLENAHEREFTYMLKATSQSEMKRWMTSLVPNRRTKFVSMSAKLLDCPQVQCVHPYVSQEPDELSLDLADILNVLDKTEDGWVFGERLHDQEKGWFPRTVVEEILNPNIRTQNLKECFRVHKPEEGSQNKDRRKMGSRTRQ

Target ORF information:

RefSeq Version	XM_002933353.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933353.2

ORF Insert Sequence:

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ngef ( XM_031902564.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758424.1 Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16053
Clone ID Related Accession (Same CDS sequence)	XM_002933353.2 , XM_031902564.1 , XM_002933353.3
Accession Version	XM_031902564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758424.1
CDS	207..2282
Translation	MRRFMRETLTQSRDSRMGRDALTASSVINMREPQIKNEEESEDENQMDESDSQTRHMPIRRNSRFYRSMRVRNRHRRDRLSSREGMEIDNQTTAQQTYDGSRSIHLSVHYNPTTGPAGYREYLPSEDTSNPHEDSSSPSECATSNSEAPPTIYPLPSDSFKTIIDHIEFLYQEYRDKSTQHEIETRRLQDNYVQSAETSNNSQNTPLGSPPPIENQRANSTFSRFESSCSGFNLWQDLPDVRKSGVLALLQPEEVKLQEAMFELLTSEASYYKSLRLVVSHFMDSERLRASLHQSEIHFIFSNVLEVLATSERFLLALEQRFEENIVISDVCDIVYEHAEKHFVVYVTYVCNQTYQERAYRKLLQERPEFRESVGHLETDPVCRGLPFSSFLILPFQRITRLKLLVQNILTKVEEGSEREVSALQAHRELEKLVRACNDGVRKMSRTEQLISIEKTLEFKIKSVPIISHSRWLMKQGELQQMSGPRTSRTLRTKKLFRPVYLFLFNDLLLMCKPLTGERYQVFDVAARGLLRVEELEDQGQTLANVFILKLLENAHEREFTYMLKATSQSEMKRWMTSLVPNRRTKFVSMSAKLLDCPQVQCVHPYVSQEPDELSLDLADILNVLDKTEDGWVFGERLHDQEKGWFPRTVVEEILNPNIRTQNLKECFRVHKPEEGSQNKDRRKMGSRTRQ

Target ORF information:

RefSeq Version	XM_031902564.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902564.1

ORF Insert Sequence:

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ngef ( XM_002933353.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933399.1 Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16053
Clone ID Related Accession (Same CDS sequence)	XM_002933353.2 , XM_031902564.1 , XM_002933353.3
Accession Version	XM_002933353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933399.1
CDS	200..2275
Translation	MRRFMRETLTQSRDSRMGRDALTASSVINMREPQIKNEEESEDENQMDESDSQTRHMPIRRNSRFYRSMRVRNRHRRDRLSSREGMEIDNQTTAQQTYDGSRSIHLSVHYNPTTGPAGYREYLPSEDTSNPHEDSSSPSECATSNSEAPPTIYPLPSDSFKTIIDHIEFLYQEYRDKSTQHEIETRRLQDNYVQSAETSNNSQNTPLGSPPPIENQRANSTFSRFESSCSGFNLWQDLPDVRKSGVLALLQPEEVKLQEAMFELLTSEASYYKSLRLVVSHFMDSERLRASLHQSEIHFIFSNVLEVLATSERFLLALEQRFEENIVISDVCDIVYEHAEKHFVVYVTYVCNQTYQERAYRKLLQERPEFRESVGHLETDPVCRGLPFSSFLILPFQRITRLKLLVQNILTKVEEGSEREVSALQAHRELEKLVRACNDGVRKMSRTEQLISIEKTLEFKIKSVPIISHSRWLMKQGELQQMSGPRTSRTLRTKKLFRPVYLFLFNDLLLMCKPLTGERYQVFDVAARGLLRVEELEDQGQTLANVFILKLLENAHEREFTYMLKATSQSEMKRWMTSLVPNRRTKFVSMSAKLLDCPQVQCVHPYVSQEPDELSLDLADILNVLDKTEDGWVFGERLHDQEKGWFPRTVVEEILNPNIRTQNLKECFRVHKPEEGSQNKDRRKMGSRTRQ

Target ORF information:

RefSeq Version	XM_002933353.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933353.3

ORF Insert Sequence:

ATGAGGCGTT TTATGAGAGA GACTCTGACG CAATCCAGAG ATAGCAGAAT GGGACGAGAT 
GCTCTTACAG CTTCTTCAGT CATTAACATG CGTGAACCTC AGATCAAGAA TGAAGAAGAA 
AGTGAAGATG AAAACCAGAT GGACGAGTCT GACAGTCAGA CCCGGCACAT GCCCATCCGC 
AGGAACTCTC GATTCTACAG ATCCATGAGG GTACGGAACA GGCATCGGAG GGACAGACTG 
AGCAGCAGGG AAGGAATGGA AATTGATAAC CAAACTACAG CCCAACAGAC ATATGATGGC 
TCCAGAAGTA TTCATCTCAG TGTGCACTAC AACCCCACTA CAGGCCCTGC AGGGTACAGG 
GAATATTTGC CAAGTGAAGA CACAAGCAAT CCCCATGAAG ACAGTTCGTC CCCTTCTGAG 
TGTGCCACTT CTAATTCAGA GGCACCACCA ACTATTTACC CCTTGCCCTC AGATTCCTTT 
AAAACCATTA TTGACCACAT AGAGTTCCTT TACCAGGAAT ACAGAGATAA ATCTACACAG 
CATGAGATTG AGACTCGAAG GCTACAGGAT AATTATGTAC AATCTGCTGA GACCTCCAAC 
AACTCTCAGA ACACACCTTT GGGCTCCCCG CCACCGATCG AGAATCAAAG GGCAAACTCT 
ACATTTAGCC GGTTCGAGAG CTCCTGTTCT GGATTTAACT TGTGGCAGGA CCTCCCGGAC 
GTCAGGAAAA GTGGAGTGCT GGCTCTGTTG CAGCCCGAAG AAGTTAAACT CCAGGAGGCC 
ATGTTTGAGC TGTTGACCTC TGAAGCCTCG TACTACAAGA GCCTTCGCTT GGTGGTATCT 
CACTTCATGG ACAGTGAGCG ACTGCGAGCG TCACTGCACC AGTCAGAGAT TCACTTCATC 
TTCTCAAATG TATTGGAGGT TCTGGCCACT AGTGAGAGGT TCCTCCTGGC CCTGGAGCAA 
CGGTTTGAGG AAAACATTGT GATATCAGAT GTGTGTGACA TCGTTTATGA GCATGCTGAG 
AAACATTTTG TCGTTTATGT CACCTACGTG TGCAATCAGA CCTATCAGGA GAGAGCGTAT 
AGAAAGCTCC TTCAGGAGAG GCCAGAATTC CGAGAATCGG TGGGACATTT GGAGACAGAT 
CCTGTATGCC GTGGGCTTCC TTTTTCATCA TTTCTCATCC TTCCATTCCA GAGGATCACA 
CGACTCAAGC TTCTGGTGCA GAATATCCTG ACCAAGGTGG AAGAAGGCTC AGAGCGAGAA 
GTGTCAGCTC TACAGGCACA CCGGGAGCTG GAGAAGTTAG TGCGTGCCTG CAATGACGGG 
GTGAGGAAGA TGAGCCGCAC AGAGCAGCTG ATCAGCATAG AGAAGACGCT TGAGTTTAAG 
ATTAAGTCTG TCCCCATCAT TTCACACTCA CGCTGGCTAA TGAAGCAGGG AGAGCTACAG 
CAGATGTCAG GGCCACGTAC GTCCCGCACT CTACGCACTA AGAAGCTTTT CCGCCCAGTC 
TATCTCTTCC TATTCAATGA CCTTCTCCTG ATGTGCAAGC CTCTCACAGG GGAACGATAT 
CAGGTGTTTG ATGTGGCTGC TCGGGGTCTG CTGCGTGTAG AGGAGCTGGA GGATCAGGGC 
CAGACACTGG CCAATGTCTT CATTCTGAAG CTTCTGGAGA ATGCACATGA AAGGGAATTC 
ACCTACATGC TAAAGGCCAC CTCCCAGAGT GAGATGAAGC GCTGGATGAC ATCACTGGTT 
CCAAACCGAA GAACCAAGTT TGTCTCAATG TCCGCAAAGC TTCTGGACTG CCCTCAGGTA 
CAGTGTGTCC ATCCGTACGT TTCCCAGGAG CCAGATGAGC TGTCGCTAGA CCTTGCCGAT 
ATTCTCAACG TCCTGGATAA AACGGAAGAC GGTTGGGTTT TCGGAGAGCG TTTGCATGAC 
CAGGAGAAAG GCTGGTTCCC CCGCACCGTT GTAGAAGAAA TACTGAACCC CAATATCCGG 
ACACAGAACC TCAAGGAATG TTTTCGGGTG CACAAACCTG AGGAAGGCAG CCAGAACAAA 
GACCGACGGA AGATGGGAAG CAGAACACGG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ngef ( XM_004917796.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917853.1 Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16054
Clone ID Related Accession (Same CDS sequence)	XM_004917796.4 , XM_004917796.3
Accession Version	XM_004917796.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephexin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917796.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCCC TGGCAGTGTC CCAGCAGGAG ACAGAAGATC CTGCATTTGA CCCTTTAAGC 
CCTGGCGAGG AAGACCCCAC AGAAGGGGAA TATTTGCCAA GTGAAGACAC AAGCAATCCC 
CATGAAGACA GTTCGTCCCC TTCTGAGTGT GCCACTTCTA ATTCAGAGGC ACCACCAACT 
ATTTACCCCT TGCCCTCAGA TTCCTTTAAA ACCATTATTG ACCACATAGA GTTCCTTTAC 
CAGGAATACA GAGATAAATC TACACAGCAT GAGATTGAGA CTCGAAGGCT ACAGGATAAT 
TATGTACAAT CTGCTGAGAC CTCCAACAAC TCTCAGAACA CACCTTTGGG CTCCCCGCCA 
CCGATCGAGA ATCAAAGGGC AAACTCTACA TTTAGCCGGT TCGAGAGCTC CTGTTCTGGA 
TTTAACTTGT GGCAGGACCT CCCGGACGTC AGGAAAAGTG GAGTGCTGGC TCTGTTGCAG 
CCCGAAGAAG TTAAACTCCA GGAGGCCATG TTTGAGCTGT TGACCTCTGA AGCCTCGTAC 
TACAAGAGCC TTCGCTTGGT GGTATCTCAC TTCATGGACA GTGAGCGACT GCGAGCGTCA 
CTGCACCAGT CAGAGATTCA CTTCATCTTC TCAAATGTAT TGGAGGTTCT GGCCACTAGT 
GAGAGGTTCC TCCTGGCCCT GGAGCAACGG TTTGAGGAAA ACATTGTGAT ATCAGATGTG 
TGTGACATCG TTTATGAGCA TGCTGAGAAA CATTTTGTCG TTTATGTCAC CTACGTGTGC 
AATCAGACCT ATCAGGAGAG AGCGTATAGA AAGCTCCTTC AGGAGAGGCC AGAATTCCGA 
GAATCGGTGG GACATTTGGA GACAGATCCT GTATGCCGTG GGCTTCCTTT TTCATCATTT 
CTCATCCTTC CATTCCAGAG GATCACACGA CTCAAGCTTC TGGTGCAGAA TATCCTGACC 
AAGGTGGAAG AAGGCTCAGA GCGAGAAGTG TCAGCTCTAC AGGCACACCG GGAGCTGGAG 
AAGTTAGTGC GTGCCTGCAA TGACGGGGTG AGGAAGATGA GCCGCACAGA GCAGCTGATC 
AGCATAGAGA AGACGCTTGA GTTTAAGATT AAGTCTGTCC CCATCATTTC ACACTCACGC 
TGGCTAATGA AGCAGGGAGA GCTACAGCAG ATGTCAGGGC CACGTACGTC CCGCACTCTA 
CGCACTAAGA AGCTTTTCCG CCCAGTCTAT CTCTTCCTAT TCAATGACCT TCTCCTGATG 
TGCAAGCCTC TCACAGGGGA ACGATATCAG GTGTTTGATG TGGCTGCTCG GGGTCTGCTG 
CGTGTAGAGG AGCTGGAGGA TCAGGGCCAG ACACTGGCCA ATGTCTTCAT TCTGAAGCTT 
CTGGAGAATG CACATGAAAG GGAATTCACC TACATGCTAA AGGCCACCTC CCAGAGTGAG 
ATGAAGCGCT GGATGACATC ACTGGTTCCA AACCGAAGAA CCAAGTTTGT CTCAATGTCC 
GCAAAGCTTC TGGACTGCCC TCAGGTACAG TGTGTCCATC CGTACGTTTC CCAGGAGCCA 
GATGAGCTGT CGCTAGACCT TGCCGATATT CTCAACGTCC TGGATAAAAC GGAAGACGGT 
TGGGTTTTCG GAGAGCGTTT GCATGACCAG GAGAAAGGCT GGTTCCCCCG CACCGTTGTA 
GAAGAAATAC TGAACCCCAA TATCCGGACA CAGAACCTCA AGGAATGTTT TCGGGTGCAC 
AAACCTGAGG AAGGCAGCCA GAACAAAGAC CGACGGAAGA TGGGAAGCAG AACACGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917853.1
CDS	237..2039
Translation	MEPLAVSQQETEDPAFDPLSPGEEDPTEGEYLPSEDTSNPHEDSSSPSECATSNSEAPPTIYPLPSDSFKTIIDHIEFLYQEYRDKSTQHEIETRRLQDNYVQSAETSNNSQNTPLGSPPPIENQRANSTFSRFESSCSGFNLWQDLPDVRKSGVLALLQPEEVKLQEAMFELLTSEASYYKSLRLVVSHFMDSERLRASLHQSEIHFIFSNVLEVLATSERFLLALEQRFEENIVISDVCDIVYEHAEKHFVVYVTYVCNQTYQERAYRKLLQERPEFRESVGHLETDPVCRGLPFSSFLILPFQRITRLKLLVQNILTKVEEGSEREVSALQAHRELEKLVRACNDGVRKMSRTEQLISIEKTLEFKIKSVPIISHSRWLMKQGELQQMSGPRTSRTLRTKKLFRPVYLFLFNDLLLMCKPLTGERYQVFDVAARGLLRVEELEDQGQTLANVFILKLLENAHEREFTYMLKATSQSEMKRWMTSLVPNRRTKFVSMSAKLLDCPQVQCVHPYVSQEPDELSLDLADILNVLDKTEDGWVFGERLHDQEKGWFPRTVVEEILNPNIRTQNLKECFRVHKPEEGSQNKDRRKMGSRTRQ

Target ORF information:

RefSeq Version	XM_004917796.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917796.4

ORF Insert Sequence:

ATGGAACCCC TGGCAGTGTC CCAGCAGGAG ACAGAAGATC CTGCATTTGA CCCTTTAAGC 
CCTGGCGAGG AAGACCCCAC AGAAGGGGAA TATTTGCCAA GTGAAGACAC AAGCAATCCC 
CATGAAGACA GTTCGTCCCC TTCTGAGTGT GCCACTTCTA ATTCAGAGGC ACCACCAACT 
ATTTACCCCT TGCCCTCAGA TTCCTTTAAA ACCATTATTG ACCACATAGA GTTCCTTTAC 
CAGGAATACA GAGATAAATC TACACAGCAT GAGATTGAGA CTCGAAGGCT ACAGGATAAT 
TATGTACAAT CTGCTGAGAC CTCCAACAAC TCTCAGAACA CACCTTTGGG CTCCCCGCCA 
CCGATCGAGA ATCAAAGGGC AAACTCTACA TTTAGCCGGT TCGAGAGCTC CTGTTCTGGA 
TTTAACTTGT GGCAGGACCT CCCGGACGTC AGGAAAAGTG GAGTGCTGGC TCTGTTGCAG 
CCCGAAGAAG TTAAACTCCA GGAGGCCATG TTTGAGCTGT TGACCTCTGA AGCCTCGTAC 
TACAAGAGCC TTCGCTTGGT GGTATCTCAC TTCATGGACA GTGAGCGACT GCGAGCGTCA 
CTGCACCAGT CAGAGATTCA CTTCATCTTC TCAAATGTAT TGGAGGTTCT GGCCACTAGT 
GAGAGGTTCC TCCTGGCCCT GGAGCAACGG TTTGAGGAAA ACATTGTGAT ATCAGATGTG 
TGTGACATCG TTTATGAGCA TGCTGAGAAA CATTTTGTCG TTTATGTCAC CTACGTGTGC 
AATCAGACCT ATCAGGAGAG AGCGTATAGA AAGCTCCTTC AGGAGAGGCC AGAATTCCGA 
GAATCGGTGG GACATTTGGA GACAGATCCT GTATGCCGTG GGCTTCCTTT TTCATCATTT 
CTCATCCTTC CATTCCAGAG GATCACACGA CTCAAGCTTC TGGTGCAGAA TATCCTGACC 
AAGGTGGAAG AAGGCTCAGA GCGAGAAGTG TCAGCTCTAC AGGCACACCG GGAGCTGGAG 
AAGTTAGTGC GTGCCTGCAA TGACGGGGTG AGGAAGATGA GCCGCACAGA GCAGCTGATC 
AGCATAGAGA AGACGCTTGA GTTTAAGATT AAGTCTGTCC CCATCATTTC ACACTCACGC 
TGGCTAATGA AGCAGGGAGA GCTACAGCAG ATGTCAGGGC CACGTACGTC CCGCACTCTA 
CGCACTAAGA AGCTTTTCCG CCCAGTCTAT CTCTTCCTAT TCAATGACCT TCTCCTGATG 
TGCAAGCCTC TCACAGGGGA ACGATATCAG GTGTTTGATG TGGCTGCTCG GGGTCTGCTG 
CGTGTAGAGG AGCTGGAGGA TCAGGGCCAG ACACTGGCCA ATGTCTTCAT TCTGAAGCTT 
CTGGAGAATG CACATGAAAG GGAATTCACC TACATGCTAA AGGCCACCTC CCAGAGTGAG 
ATGAAGCGCT GGATGACATC ACTGGTTCCA AACCGAAGAA CCAAGTTTGT CTCAATGTCC 
GCAAAGCTTC TGGACTGCCC TCAGGTACAG TGTGTCCATC CGTACGTTTC CCAGGAGCCA 
GATGAGCTGT CGCTAGACCT TGCCGATATT CTCAACGTCC TGGATAAAAC GGAAGACGGT 
TGGGTTTTCG GAGAGCGTTT GCATGACCAG GAGAAAGGCT GGTTCCCCCG CACCGTTGTA 
GAAGAAATAC TGAACCCCAA TATCCGGACA CAGAACCTCA AGGAATGTTT TCGGGTGCAC 
AAACCTGAGG AAGGCAGCCA GAACAAAGAC CGACGGAAGA TGGGAAGCAG AACACGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ngef ( XM_004917796.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917853.1 Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16054
Clone ID Related Accession (Same CDS sequence)	XM_004917796.4 , XM_004917796.3
Accession Version	XM_004917796.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ephexin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917796.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCCC TGGCAGTGTC CCAGCAGGAG ACAGAAGATC CTGCATTTGA CCCTTTAAGC 
CCTGGCGAGG AAGACCCCAC AGAAGGGGAA TATTTGCCAA GTGAAGACAC AAGCAATCCC 
CATGAAGACA GTTCGTCCCC TTCTGAGTGT GCCACTTCTA ATTCAGAGGC ACCACCAACT 
ATTTACCCCT TGCCCTCAGA TTCCTTTAAA ACCATTATTG ACCACATAGA GTTCCTTTAC 
CAGGAATACA GAGATAAATC TACACAGCAT GAGATTGAGA CTCGAAGGCT ACAGGATAAT 
TATGTACAAT CTGCTGAGAC CTCCAACAAC TCTCAGAACA CACCTTTGGG CTCCCCGCCA 
CCGATCGAGA ATCAAAGGGC AAACTCTACA TTTAGCCGGT TCGAGAGCTC CTGTTCTGGA 
TTTAACTTGT GGCAGGACCT CCCGGACGTC AGGAAAAGTG GAGTGCTGGC TCTGTTGCAG 
CCCGAAGAAG TTAAACTCCA GGAGGCCATG TTTGAGCTGT TGACCTCTGA AGCCTCGTAC 
TACAAGAGCC TTCGCTTGGT GGTATCTCAC TTCATGGACA GTGAGCGACT GCGAGCGTCA 
CTGCACCAGT CAGAGATTCA CTTCATCTTC TCAAATGTAT TGGAGGTTCT GGCCACTAGT 
GAGAGGTTCC TCCTGGCCCT GGAGCAACGG TTTGAGGAAA ACATTGTGAT ATCAGATGTG 
TGTGACATCG TTTATGAGCA TGCTGAGAAA CATTTTGTCG TTTATGTCAC CTACGTGTGC 
AATCAGACCT ATCAGGAGAG AGCGTATAGA AAGCTCCTTC AGGAGAGGCC AGAATTCCGA 
GAATCGGTGG GACATTTGGA GACAGATCCT GTATGCCGTG GGCTTCCTTT TTCATCATTT 
CTCATCCTTC CATTCCAGAG GATCACACGA CTCAAGCTTC TGGTGCAGAA TATCCTGACC 
AAGGTGGAAG AAGGCTCAGA GCGAGAAGTG TCAGCTCTAC AGGCACACCG GGAGCTGGAG 
AAGTTAGTGC GTGCCTGCAA TGACGGGGTG AGGAAGATGA GCCGCACAGA GCAGCTGATC 
AGCATAGAGA AGACGCTTGA GTTTAAGATT AAGTCTGTCC CCATCATTTC ACACTCACGC 
TGGCTAATGA AGCAGGGAGA GCTACAGCAG ATGTCAGGGC CACGTACGTC CCGCACTCTA 
CGCACTAAGA AGCTTTTCCG CCCAGTCTAT CTCTTCCTAT TCAATGACCT TCTCCTGATG 
TGCAAGCCTC TCACAGGGGA ACGATATCAG GTGTTTGATG TGGCTGCTCG GGGTCTGCTG 
CGTGTAGAGG AGCTGGAGGA TCAGGGCCAG ACACTGGCCA ATGTCTTCAT TCTGAAGCTT 
CTGGAGAATG CACATGAAAG GGAATTCACC TACATGCTAA AGGCCACCTC CCAGAGTGAG 
ATGAAGCGCT GGATGACATC ACTGGTTCCA AACCGAAGAA CCAAGTTTGT CTCAATGTCC 
GCAAAGCTTC TGGACTGCCC TCAGGTACAG TGTGTCCATC CGTACGTTTC CCAGGAGCCA 
GATGAGCTGT CGCTAGACCT TGCCGATATT CTCAACGTCC TGGATAAAAC GGAAGACGGT 
TGGGTTTTCG GAGAGCGTTT GCATGACCAG GAGAAAGGCT GGTTCCCCCG CACCGTTGTA 
GAAGAAATAC TGAACCCCAA TATCCGGACA CAGAACCTCA AGGAATGTTT TCGGGTGCAC 
AAACCTGAGG AAGGCAGCCA GAACAAAGAC CGACGGAAGA TGGGAAGCAG AACACGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917853.1
CDS	221..2023
Translation	MEPLAVSQQETEDPAFDPLSPGEEDPTEGEYLPSEDTSNPHEDSSSPSECATSNSEAPPTIYPLPSDSFKTIIDHIEFLYQEYRDKSTQHEIETRRLQDNYVQSAETSNNSQNTPLGSPPPIENQRANSTFSRFESSCSGFNLWQDLPDVRKSGVLALLQPEEVKLQEAMFELLTSEASYYKSLRLVVSHFMDSERLRASLHQSEIHFIFSNVLEVLATSERFLLALEQRFEENIVISDVCDIVYEHAEKHFVVYVTYVCNQTYQERAYRKLLQERPEFRESVGHLETDPVCRGLPFSSFLILPFQRITRLKLLVQNILTKVEEGSEREVSALQAHRELEKLVRACNDGVRKMSRTEQLISIEKTLEFKIKSVPIISHSRWLMKQGELQQMSGPRTSRTLRTKKLFRPVYLFLFNDLLLMCKPLTGERYQVFDVAARGLLRVEELEDQGQTLANVFILKLLENAHEREFTYMLKATSQSEMKRWMTSLVPNRRTKFVSMSAKLLDCPQVQCVHPYVSQEPDELSLDLADILNVLDKTEDGWVFGERLHDQEKGWFPRTVVEEILNPNIRTQNLKECFRVHKPEEGSQNKDRRKMGSRTRQ

Target ORF information:

RefSeq Version	XM_004917796.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neuronal guanine nucleotide exchange factor (ngef), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917796.3

ORF Insert Sequence:

ATGGAACCCC TGGCAGTGTC CCAGCAGGAG ACAGAAGATC CTGCATTTGA CCCTTTAAGC 
CCTGGCGAGG AAGACCCCAC AGAAGGGGAA TATTTGCCAA GTGAAGACAC AAGCAATCCC 
CATGAAGACA GTTCGTCCCC TTCTGAGTGT GCCACTTCTA ATTCAGAGGC ACCACCAACT 
ATTTACCCCT TGCCCTCAGA TTCCTTTAAA ACCATTATTG ACCACATAGA GTTCCTTTAC 
CAGGAATACA GAGATAAATC TACACAGCAT GAGATTGAGA CTCGAAGGCT ACAGGATAAT 
TATGTACAAT CTGCTGAGAC CTCCAACAAC TCTCAGAACA CACCTTTGGG CTCCCCGCCA 
CCGATCGAGA ATCAAAGGGC AAACTCTACA TTTAGCCGGT TCGAGAGCTC CTGTTCTGGA 
TTTAACTTGT GGCAGGACCT CCCGGACGTC AGGAAAAGTG GAGTGCTGGC TCTGTTGCAG 
CCCGAAGAAG TTAAACTCCA GGAGGCCATG TTTGAGCTGT TGACCTCTGA AGCCTCGTAC 
TACAAGAGCC TTCGCTTGGT GGTATCTCAC TTCATGGACA GTGAGCGACT GCGAGCGTCA 
CTGCACCAGT CAGAGATTCA CTTCATCTTC TCAAATGTAT TGGAGGTTCT GGCCACTAGT 
GAGAGGTTCC TCCTGGCCCT GGAGCAACGG TTTGAGGAAA ACATTGTGAT ATCAGATGTG 
TGTGACATCG TTTATGAGCA TGCTGAGAAA CATTTTGTCG TTTATGTCAC CTACGTGTGC 
AATCAGACCT ATCAGGAGAG AGCGTATAGA AAGCTCCTTC AGGAGAGGCC AGAATTCCGA 
GAATCGGTGG GACATTTGGA GACAGATCCT GTATGCCGTG GGCTTCCTTT TTCATCATTT 
CTCATCCTTC CATTCCAGAG GATCACACGA CTCAAGCTTC TGGTGCAGAA TATCCTGACC 
AAGGTGGAAG AAGGCTCAGA GCGAGAAGTG TCAGCTCTAC AGGCACACCG GGAGCTGGAG 
AAGTTAGTGC GTGCCTGCAA TGACGGGGTG AGGAAGATGA GCCGCACAGA GCAGCTGATC 
AGCATAGAGA AGACGCTTGA GTTTAAGATT AAGTCTGTCC CCATCATTTC ACACTCACGC 
TGGCTAATGA AGCAGGGAGA GCTACAGCAG ATGTCAGGGC CACGTACGTC CCGCACTCTA 
CGCACTAAGA AGCTTTTCCG CCCAGTCTAT CTCTTCCTAT TCAATGACCT TCTCCTGATG 
TGCAAGCCTC TCACAGGGGA ACGATATCAG GTGTTTGATG TGGCTGCTCG GGGTCTGCTG 
CGTGTAGAGG AGCTGGAGGA TCAGGGCCAG ACACTGGCCA ATGTCTTCAT TCTGAAGCTT 
CTGGAGAATG CACATGAAAG GGAATTCACC TACATGCTAA AGGCCACCTC CCAGAGTGAG 
ATGAAGCGCT GGATGACATC ACTGGTTCCA AACCGAAGAA CCAAGTTTGT CTCAATGTCC 
GCAAAGCTTC TGGACTGCCC TCAGGTACAG TGTGTCCATC CGTACGTTTC CCAGGAGCCA 
GATGAGCTGT CGCTAGACCT TGCCGATATT CTCAACGTCC TGGATAAAAC GGAAGACGGT 
TGGGTTTTCG GAGAGCGTTT GCATGACCAG GAGAAAGGCT GGTTCCCCCG CACCGTTGTA 
GAAGAAATAC TGAACCCCAA TATCCGGACA CAGAACCTCA AGGAATGTTT TCGGGTGCAC 
AAACCTGAGG AAGGCAGCCA GAACAAAGAC CGACGGAAGA TGGGAAGCAG AACACGGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ngef cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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