NGEF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NGEF
Entrez Gene ID	540756
Full Name	neuronal guanine nucleotide exchange factor
General protein information	Preferred Names neuronal guanine nucleotide exchange factor Names ephexin-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076926.1	NP_001070394.1	ephexin-1
XM_024989649.1	XP_024845417.1	ephexin-1 isoform X1
XM_024989652.1	XP_024845420.1	ephexin-1 isoform X1
XM_024989651.1	XP_024845419.1	ephexin-1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NGEF	XP_005635921.1
Danio rerio (zebrafish)	LOC569090	XP_697546.3
Xenopus tropicalis (tropical clawed frog)	ngef	XP_004917852.1
Bos taurus (cattle)	NGEF	NP_001070394.1
Rattus norvegicus (Norway rat)	Ngef	NP_001129713.1
Pan troglodytes (chimpanzee)	NGEF	XP_516157.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007452	XP_308411.4
Homo sapiens (human)	NGEF	NP_062824.2
Macaca mulatta (Rhesus monkey)	LOC713917	XP_001114604.1
Mus musculus (house mouse)	Ngef	NP_001104784.1
Gallus gallus (chicken)	NGEF	NP_001010841.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40304	NM_001076926.1 Latest version!	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa232949	XM_024989652.1 Latest version!	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa232949	XM_024989649.1 Latest version!	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa232949	XM_024989651.1 Latest version!	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NGEF ( NM_001076926.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001070394.1 Bos taurus neuronal guanine nucleotide exchange factor (NGEF), mRNA.

Price & Availability↑

CloneID	OBa40304
Clone ID Related Accession (Same CDS sequence)	NM_001076926.1
Accession Version	NM_001076926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	ephexin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123858.1. On Oct 11, 2006 this sequence version replaced XM_612979.2. ##Evidence-Data-START## Transcript exon combination :: BC123858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTGC TGGCCGCAGC CTTCAACGCC GCCTGCGCCG TGGACCACGA CAGCTCCACC 
TCGGAGAGCG ACGCGCGCGA CTCAGCGGCG GGACACCTGC CCGGCAGCGA GTCGTCCTCT 
ACCCCAGGAA ATGGGGCTAC GCCCGAGGAG TTCCCGGCCC TCGCAGACAG CCCCACCACG 
CTCACCGAGG CCTTGCAGAT GATCCACCCT ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CAACTCTCCA AGAGATTGAG 
ACCAGGAGGC AACAGGATGC AGAAATACAA GGTGCTGCTG ACGGGTGCCC ATCCAGCGAG 
GAGGCTCCAG AGGAAGATGA AGGGGAGGAG GAGGAACCAG CAAGCCCACC TGAGAGGAAG 
GCCCTGCCCC AGATCTGCCT GCTCAGCAAC CCCCACTCGA GATTCAACCT CTGGCAGGAT 
CTCCCGGAGA TTCAGAGCAG CGGGGTGCTG GACATCCTCC AGCCGGAGGA GATCAGGCTG 
CAAGAGGCCA TGTTCGAGCT CATCACTTCT GAGGCCTCCT ACTATAAAAG CCTGAACCTT 
CTGGTGTCCC ACTTCATGGA GAACGAGCGG CTGAAGAAGA TCCTGCACCC GTCTGAGGCC 
CACATCCTCT TCTCCAACGT GCTGGATGTC ATGGCCGTCA GTGAGCGGTT TCTGTTGGAG 
CTGGAGCGCC GGATGGAGGA AAACATCGTC ATCTCGGACG TGTGTGACAT CGTGTACCGC 
TACGCCGCTG ACCACTTCTC CGTCTACATC ACCTACGTCA GCAACCAGAC CTACCAGGAG 
CGGACCTATA AGCAGCTGCT CCAGGAGAAG GCAGCCTTCC GGGAGCTGAT CGCGCAGCTG 
GAGTTGGACC CCAAGTGCAG GGGGCTGCCC CTCTCCTCCT TCCTCATCCT GCCTTTCCAG 
AGGATCACGC GCCTCAAGCT GTTGGTCCAG AACATCCTGA AGAGGGTGGA GGAAAGGTCT 
GAGCGCGAAG GCACCGCCTT GGACGCCCAC AAGGAACTGG AGCTGGTGGT GAAAGCATGC 
AACGAGGGCG TCAGGAAGAT GAGCCGCACG GAGCAGATGA TCAGCATCCA GAAGAAGATG 
GAGTTCAAGA TCAAGTCGGT GCCCATCATC TCACACTCCC GCTGGCTGCT GAAGCAGGGC 
GAGCTGCAGC AGATGTCCGG CCCCAAGACC TCGCGGACCC TGCGGACCAA GAAACTCTTC 
CGGGAGATTT ACCTCTTTCT CTTCAACGAC CTGCTGGTGA TTTGCCGGCA GATTCCAGGA 
GACAAGTACC AGGTGTTTGA CTCGGCCCCG CGAGGCCTGC TGCGCGCGGA GGAACTGGAG 
GACCAGGGCC AGACGCTGGC CAACGTCTTC ATCCTACGGC TGCTCGAGAA CTCAGACGAT 
CGGGAGGCCA CTTACATGCT GAAGGCGTCC TCTCAGAGCG AGATGAAGCG CTGGATGACC 
TCGCTGGCCC CCAACCGAAG AACTAAGTTC GTTTCCTTCA CGTCCCGGCT GCTCGACTGC 
CCCCAGGTCC AGTGTGTGCA CCCGTATGTG GCCCAGCAGC CGGATGAGCT GACGCTGGAG 
CTGGCCGACA TCCTCAACAT CCTGGACAAG ACAGAGGACG GCTGGATCTT TGGCGAGCGT 
CTGCATGACC AGGAGCGAGG CTGGTTCCCC AGCAATATGA CCGAGGAGAT CTTGAACCCC 
AAGATCCGGT CCCAGAACCT CAAGGAGTGT TTCCGTGTCC ACAAGATGGA CGACCCTCAG 
CGCAGCCAGA ATAAGGACCG CAGGAAGCTG GGCAGCCGGA ATCGGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070394.1
CDS	125..1975
Translation	MELLAAAFNAACAVDHDSSTSESDARDSAAGHLPGSESSSTPGNGATPEEFPALADSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGAADGCPSSEEAPEEDEGEEEEPASPPERKALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELITSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELERRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPLSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELELVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRAEELEDQGQTLANVFILRLLENSDDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTEDGWIFGERLHDQERGWFPSNMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	NM_001076926.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076926.1

ORF Insert Sequence:

ATGGAGCTGC TGGCCGCAGC CTTCAACGCC GCCTGCGCCG TGGACCACGA CAGCTCCACC 
TCGGAGAGCG ACGCGCGCGA CTCAGCGGCG GGACACCTGC CCGGCAGCGA GTCGTCCTCT 
ACCCCAGGAA ATGGGGCTAC GCCCGAGGAG TTCCCGGCCC TCGCAGACAG CCCCACCACG 
CTCACCGAGG CCTTGCAGAT GATCCACCCT ATTCCCGCCG ACTCCTGGAG AAACCTCATT 
GAACAAATAG GGCTCCTGTA TCAGGAATAC CGAGATAAAT CAACTCTCCA AGAGATTGAG 
ACCAGGAGGC AACAGGATGC AGAAATACAA GGTGCTGCTG ACGGGTGCCC ATCCAGCGAG 
GAGGCTCCAG AGGAAGATGA AGGGGAGGAG GAGGAACCAG CAAGCCCACC TGAGAGGAAG 
GCCCTGCCCC AGATCTGCCT GCTCAGCAAC CCCCACTCGA GATTCAACCT CTGGCAGGAT 
CTCCCGGAGA TTCAGAGCAG CGGGGTGCTG GACATCCTCC AGCCGGAGGA GATCAGGCTG 
CAAGAGGCCA TGTTCGAGCT CATCACTTCT GAGGCCTCCT ACTATAAAAG CCTGAACCTT 
CTGGTGTCCC ACTTCATGGA GAACGAGCGG CTGAAGAAGA TCCTGCACCC GTCTGAGGCC 
CACATCCTCT TCTCCAACGT GCTGGATGTC ATGGCCGTCA GTGAGCGGTT TCTGTTGGAG 
CTGGAGCGCC GGATGGAGGA AAACATCGTC ATCTCGGACG TGTGTGACAT CGTGTACCGC 
TACGCCGCTG ACCACTTCTC CGTCTACATC ACCTACGTCA GCAACCAGAC CTACCAGGAG 
CGGACCTATA AGCAGCTGCT CCAGGAGAAG GCAGCCTTCC GGGAGCTGAT CGCGCAGCTG 
GAGTTGGACC CCAAGTGCAG GGGGCTGCCC CTCTCCTCCT TCCTCATCCT GCCTTTCCAG 
AGGATCACGC GCCTCAAGCT GTTGGTCCAG AACATCCTGA AGAGGGTGGA GGAAAGGTCT 
GAGCGCGAAG GCACCGCCTT GGACGCCCAC AAGGAACTGG AGCTGGTGGT GAAAGCATGC 
AACGAGGGCG TCAGGAAGAT GAGCCGCACG GAGCAGATGA TCAGCATCCA GAAGAAGATG 
GAGTTCAAGA TCAAGTCGGT GCCCATCATC TCACACTCCC GCTGGCTGCT GAAGCAGGGC 
GAGCTGCAGC AGATGTCCGG CCCCAAGACC TCGCGGACCC TGCGGACCAA GAAACTCTTC 
CGGGAGATTT ACCTCTTTCT CTTCAACGAC CTGCTGGTGA TTTGCCGGCA GATTCCAGGA 
GACAAGTACC AGGTGTTTGA CTCGGCCCCG CGAGGCCTGC TGCGCGCGGA GGAACTGGAG 
GACCAGGGCC AGACGCTGGC CAACGTCTTC ATCCTACGGC TGCTCGAGAA CTCAGACGAT 
CGGGAGGCCA CTTACATGCT GAAGGCGTCC TCTCAGAGCG AGATGAAGCG CTGGATGACC 
TCGCTGGCCC CCAACCGAAG AACTAAGTTC GTTTCCTTCA CGTCCCGGCT GCTCGACTGC 
CCCCAGGTCC AGTGTGTGCA CCCGTATGTG GCCCAGCAGC CGGATGAGCT GACGCTGGAG 
CTGGCCGACA TCCTCAACAT CCTGGACAAG ACAGAGGACG GCTGGATCTT TGGCGAGCGT 
CTGCATGACC AGGAGCGAGG CTGGTTCCCC AGCAATATGA CCGAGGAGAT CTTGAACCCC 
AAGATCCGGT CCCAGAACCT CAAGGAGTGT TTCCGTGTCC ACAAGATGGA CGACCCTCAG 
CGCAGCCAGA ATAAGGACCG CAGGAAGCTG GGCAGCCGGA ATCGGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_024989649.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845417.1 Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232949
Clone ID Related Accession (Same CDS sequence)	XM_024989649.1 , XM_024989652.1 , XM_024989651.1
Accession Version	XM_024989649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845417.1
CDS	493..2496
Translation	MPHTDQEAQDKASHTKPIQRNSTFNRAVRLRSRGKARGTSEGNPGYPADPLENENSVNEPLTLNLPQSRKHLWRIPIQTDPGAERTSESSSTPGNGATPEEFPALADSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGAADGCPSSEEAPEEDEGEEEEPASPPERKALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELITSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELERRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPLSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELELVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRAEELEDQGQTLANVFILRLLENSDDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTEDGWIFGERLHDQERGWFPSNMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_024989649.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989649.1

ORF Insert Sequence:

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_024989652.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845420.1 Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa232949
Clone ID Related Accession (Same CDS sequence)	XM_024989649.1 , XM_024989652.1 , XM_024989651.1
Accession Version	XM_024989652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845420.1
CDS	337..2340
Translation	MPHTDQEAQDKASHTKPIQRNSTFNRAVRLRSRGKARGTSEGNPGYPADPLENENSVNEPLTLNLPQSRKHLWRIPIQTDPGAERTSESSSTPGNGATPEEFPALADSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGAADGCPSSEEAPEEDEGEEEEPASPPERKALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELITSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELERRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPLSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELELVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRAEELEDQGQTLANVFILRLLENSDDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTEDGWIFGERLHDQERGWFPSNMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_024989652.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989652.1

ORF Insert Sequence:

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NGEF ( XM_024989651.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845419.1 Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.

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CloneID	OBa232949
Clone ID Related Accession (Same CDS sequence)	XM_024989649.1 , XM_024989652.1 , XM_024989651.1
Accession Version	XM_024989651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	ephexin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845419.1
CDS	366..2369
Translation	MPHTDQEAQDKASHTKPIQRNSTFNRAVRLRSRGKARGTSEGNPGYPADPLENENSVNEPLTLNLPQSRKHLWRIPIQTDPGAERTSESSSTPGNGATPEEFPALADSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQQDAEIQGAADGCPSSEEAPEEDEGEEEEPASPPERKALPQICLLSNPHSRFNLWQDLPEIQSSGVLDILQPEEIRLQEAMFELITSEASYYKSLNLLVSHFMENERLKKILHPSEAHILFSNVLDVMAVSERFLLELERRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQEKAAFRELIAQLELDPKCRGLPLSSFLILPFQRITRLKLLVQNILKRVEERSEREGTALDAHKELELVVKACNEGVRKMSRTEQMISIQKKMEFKIKSVPIISHSRWLLKQGELQQMSGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRAEELEDQGQTLANVFILRLLENSDDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCPQVQCVHPYVAQQPDELTLELADILNILDKTEDGWIFGERLHDQERGWFPSNMTEEILNPKIRSQNLKECFRVHKMDDPQRSQNKDRRKLGSRNRQ

Target ORF information:

RefSeq Version	XM_024989651.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neuronal guanine nucleotide exchange factor (NGEF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989651.1

ORF Insert Sequence:

ATGCCCCATA CCGACCAGGA GGCACAAGAC AAAGCATCCC ATACCAAGCC AATCCAGAGA 
AACTCCACCT TCAATCGCGC CGTGAGACTC AGAAGCAGAG GCAAAGCCAG AGGCACTTCT 
GAGGGGAACC CCGGCTACCC AGCAGATCCA CTGGAAAATG AGAACTCTGT CAATGAACCC 
CTGACCTTGA ATTTGCCGCA GAGCAGAAAG CACCTCTGGA GGATACCAAT CCAGACAGAC 
CCAGGAGCCG AGAGAACAAG CGAGTCGTCC TCTACCCCAG GAAATGGGGC TACGCCCGAG 
GAGTTCCCGG CCCTCGCAGA CAGCCCCACC ACGCTCACCG AGGCCTTGCA GATGATCCAC 
CCTATTCCCG CCGACTCCTG GAGAAACCTC ATTGAACAAA TAGGGCTCCT GTATCAGGAA 
TACCGAGATA AATCAACTCT CCAAGAGATT GAGACCAGGA GGCAACAGGA TGCAGAAATA 
CAAGGTGCTG CTGACGGGTG CCCATCCAGC GAGGAGGCTC CAGAGGAAGA TGAAGGGGAG 
GAGGAGGAAC CAGCAAGCCC ACCTGAGAGG AAGGCCCTGC CCCAGATCTG CCTGCTCAGC 
AACCCCCACT CGAGATTCAA CCTCTGGCAG GATCTCCCGG AGATTCAGAG CAGCGGGGTG 
CTGGACATCC TCCAGCCGGA GGAGATCAGG CTGCAAGAGG CCATGTTCGA GCTCATCACT 
TCTGAGGCCT CCTACTATAA AAGCCTGAAC CTTCTGGTGT CCCACTTCAT GGAGAACGAG 
CGGCTGAAGA AGATCCTGCA CCCGTCTGAG GCCCACATCC TCTTCTCCAA CGTGCTGGAT 
GTCATGGCCG TCAGTGAGCG GTTTCTGTTG GAGCTGGAGC GCCGGATGGA GGAAAACATC 
GTCATCTCGG ACGTGTGTGA CATCGTGTAC CGCTACGCCG CTGACCACTT CTCCGTCTAC 
ATCACCTACG TCAGCAACCA GACCTACCAG GAGCGGACCT ATAAGCAGCT GCTCCAGGAG 
AAGGCAGCCT TCCGGGAGCT GATCGCGCAG CTGGAGTTGG ACCCCAAGTG CAGGGGGCTG 
CCCCTCTCCT CCTTCCTCAT CCTGCCTTTC CAGAGGATCA CGCGCCTCAA GCTGTTGGTC 
CAGAACATCC TGAAGAGGGT GGAGGAAAGG TCTGAGCGCG AAGGCACCGC CTTGGACGCC 
CACAAGGAAC TGGAGCTGGT GGTGAAAGCA TGCAACGAGG GCGTCAGGAA GATGAGCCGC 
ACGGAGCAGA TGATCAGCAT CCAGAAGAAG ATGGAGTTCA AGATCAAGTC GGTGCCCATC 
ATCTCACACT CCCGCTGGCT GCTGAAGCAG GGCGAGCTGC AGCAGATGTC CGGCCCCAAG 
ACCTCGCGGA CCCTGCGGAC CAAGAAACTC TTCCGGGAGA TTTACCTCTT TCTCTTCAAC 
GACCTGCTGG TGATTTGCCG GCAGATTCCA GGAGACAAGT ACCAGGTGTT TGACTCGGCC 
CCGCGAGGCC TGCTGCGCGC GGAGGAACTG GAGGACCAGG GCCAGACGCT GGCCAACGTC 
TTCATCCTAC GGCTGCTCGA GAACTCAGAC GATCGGGAGG CCACTTACAT GCTGAAGGCG 
TCCTCTCAGA GCGAGATGAA GCGCTGGATG ACCTCGCTGG CCCCCAACCG AAGAACTAAG 
TTCGTTTCCT TCACGTCCCG GCTGCTCGAC TGCCCCCAGG TCCAGTGTGT GCACCCGTAT 
GTGGCCCAGC AGCCGGATGA GCTGACGCTG GAGCTGGCCG ACATCCTCAA CATCCTGGAC 
AAGACAGAGG ACGGCTGGAT CTTTGGCGAG CGTCTGCATG ACCAGGAGCG AGGCTGGTTC 
CCCAGCAATA TGACCGAGGA GATCTTGAAC CCCAAGATCC GGTCCCAGAA CCTCAAGGAG 
TGTTTCCGTG TCCACAAGAT GGACGACCCT CAGCGCAGCC AGAATAAGGA CCGCAGGAAG 
CTGGGCAGCC GGAATCGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NGEF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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