prpf8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	prpf8
Entrez Gene ID	100380187
Full Name	pre-mRNA processing factor 8
Synonyms	hprp8,prp8,prpc8,rp13
General protein information	Preferred Names pre-mRNA processing factor 8 Names pre-mRNA-processing-splicing factor 8 PRP8 pre-mRNA processing factor 8 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936059.2	XP_002936105.1	pre-mRNA-processing-splicing factor 8
XM_002936059.3	XP_002936105.2	pre-mRNA-processing-splicing factor 8
XM_004911666.3	XP_004911723.1	pre-mRNA-processing-splicing factor 8
XM_004911666.4	XP_004911723.2	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
Neurospora crassa	NCU07832	XP_962953.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Macaca mulatta (Rhesus monkey)	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Homo sapiens (human)	PRPF8	NP_006436.3
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology

The following prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05611	XM_002936059.2 Latest version!	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa05611	XM_004911666.3 Latest version!	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa52155	XM_004911666.4 Latest version!	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa52155	XM_002936059.3 Latest version!	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prpf8 ( XM_002936059.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936105.1 Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05611
Clone ID Related Accession (Same CDS sequence)	XM_002936059.2 , XM_004911666.3
Accession Version	XM_002936059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002936059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936105.1
CDS	243..7250
Translation	MAGVFPYRPQAPPMPGPPSGTPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVTDWFYDHQPLRDNRKYVNGTTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKDTPLYSDNTANGIALLWAPRPFNLRSGRTRRAIDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWDTTEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDVATESAHPIRLFCRYIDRIHIFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGGKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWVHTQPNESPQLSPQDVTTHAKVMADNPAWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_002936059.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936059.2

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prpf8 ( XM_004911666.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911723.1 Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

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CloneID	OXa05611
Clone ID Related Accession (Same CDS sequence)	XM_002936059.2 , XM_004911666.3
Accession Version	XM_004911666.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911723.1
CDS	105..7112
Translation	MAGVFPYRPQAPPMPGPPSGTPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVTDWFYDHQPLRDNRKYVNGTTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKDTPLYSDNTANGIALLWAPRPFNLRSGRTRRAIDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWDTTEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDVATESAHPIRLFCRYIDRIHIFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGGKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWVHTQPNESPQLSPQDVTTHAKVMADNPAWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_004911666.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911666.3

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAAGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGACAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACAGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CTATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA GGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAATGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCATGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AATCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACGGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACGGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATATC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prpf8 ( XM_002936059.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936105.2 Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

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CloneID	OXa52155
Clone ID Related Accession (Same CDS sequence)	XM_002936059.3 , XM_004911666.4
Accession Version	XM_002936059.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936105.2
CDS	243..7250
Translation	MAGVFPYRPQAPPMPGPPGGTPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVTDWFYDHQPLRDNRKYVNGTTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKDTPLYSDNTANGIALLWAPRPFNLRSGRTRRAIDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWDTTEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDVATESAHPIRLFCRYIDRIHIFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGGKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWVHTQPNESPQLSPQDVTTHAKVMADNPAWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_002936059.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936059.3

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prpf8 ( XM_004911666.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911723.2 Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

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CloneID	OXa52155
Clone ID Related Accession (Same CDS sequence)	XM_002936059.3 , XM_004911666.4
Accession Version	XM_004911666.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911723.2
CDS	107..7114
Translation	MAGVFPYRPQAPPMPGPPGGTPDYMSEEKLQEKARKWQQLQAKRYSEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYIAQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVTDWFYDHQPLRDNRKYVNGTTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEYVEPFLKDTPLYSDNTANGIALLWAPRPFNLRSGRTRRAIDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWDTTEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDVATESAHPIRLFCRYIDRIHIFFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGGKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWVHTQPNESPQLSPQDVTTHAKVMADNPAWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQASWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_004911666.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis pre-mRNA processing factor 8 (prpf8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911666.4

ORF Insert Sequence:

ATGGCCGGGG TGTTTCCATA TAGACCACAG GCACCGCCAA TGCCCGGCCC CCCAGGTGGA 
ACACCGGACT ACATGTCTGA AGAGAAGCTG CAGGAAAAAG CACGAAAATG GCAGCAGCTA 
CAGGCAAAAC GCTATTCAGA GAAAAGAAAA TTTGGGTTTG TGGATGCACA GAAAGAAGAT 
ATGCCTCCAG AACATGTTCG CAAAATTATT CGTGATCATG GAGACATGAC CAACAGAAAG 
TTCCGACATG ATAAGAGAGT TTACCTTGGA GCATTGAAGT ATATGCCACA TGCTGTCTTA 
AAGTTACTTG AAAACATGCC CATGCCTTGG GAGCAGATCC GTGATGTCCC TGTGCTGTAT 
CATATAACAG GAGCCATCTC ATTTGTTAAT GAGATTCCAT GGGTCATTGA GCCTGTCTAC 
ATAGCACAGT GGGGGTCAAT GTGGATTATG ATGAGGCGAG AAAAAAGAGA CAGACGGCAC 
TTCAAGAGGA TGAGGTTTCC TCCATTTGAT GATGAGGAGC CTCCACTGGA TTATGCTGAC 
AATATCCTTG ATGTTGAACC CCTGGAAGCC ATTCAGCTTG AACTGGACCC AGAGGAAGAT 
GCTCCAGTCA CTGACTGGTT CTATGATCAC CAGCCCTTAA GAGACAACAG AAAGTATGTG 
AATGGTACTA CTTATCAGCG GTGGCAGTTT ACACTTCCGA TGATGTCTAC ATTATATCGA 
TTGGCTAATC AGCTTCTGAC TGACCTGGTT GATGATAACT ACTTCTACCT GTTTGACCTA 
AAGGCATTCT TTACCTCCAA AGCATTAAAC ATGGCTATTC CTGGAGGGCC AAAATTTGAG 
CCTCTTGTTC GTGACATTAA CCTGCAGGAT GAAGATTGGA ATGAGTTTAA TGATATTAAC 
AAGATAATTA TCCGACAGCC AATTCGAACA GAATACAAGA TTGCTTTTCC TTACCTGTAC 
AACAATCTTC CTCACCATGT ACATTTAACC TGGTATCACA CACCAAATGT GGTTTTTATC 
AAAACTGAGG ATCCTGATTT ACCAGCATTC TATTTTGACC CACTGATTAA TCCCATATCA 
CACAGGCATT CTGTAAAGAG CCAAGAGCCA TTGCCAGATG ATGATGAGGA ATTTGAATTG 
CCAGAATATG TTGAACCATT CCTTAAAGAC ACTCCTCTGT ATTCAGATAA CACCGCTAAT 
GGAATTGCTT TACTTTGGGC TCCTCGTCCT TTCAACTTGC GTTCTGGTAG GACCAGGAGG 
GCTATTGATA TTCCTCTTGT GAAAAACTGG TACAGAGAGC ATTGTCCTGC TGGCCAGCCA 
GTAAAAGTCA GAGTATCTTA CCAGAAATTG CTGAAGTACT ATGTGCTAAA TGCTTTGAAA 
CACAGGCCTC CAAAAGCTCA GAAGAAAAGG TATCTCTTCC GTTCATTTAA AGCCACAAAA 
TTCTTTCAGT CGACAAAGTT GGACTGGGTG GAAGTAGGCC TGCAGGTTTG CCGCCAAGGC 
TATAACATGC TGAACTTGCT GATTCACAGA AAGAACCTTA ACTATCTTCA TCTGGATTAC 
AATTTCAATT TGAAGCCAGT AAAAACGCTG ACCACAAAGG AACGCAAAAA GTCTCGGTTC 
GGCAATGCCT TCCATCTTTG CCGCGAGGTC CTGCGTCTAA CCAAACTAGT TGTGGACAGC 
CATGTGCAAT ACAGACTGGG GAATGTTGAT GCTTTCCAGT TGGCAGACGG ATTACAGTAT 
ATTTTTGCCC ATGTGGGGCA GCTGACTGGA ATGTACCGTT ACAAGTACAA ACTAATGAGA 
CAAATTCGCA TGTGCAAGGA CCTGAAGCAT CTGATCTACT ACAGGTTCAA TACAGGTCCA 
GTTGGAAAGG GGCCTGGCTG TGGATTTTGG GCAGCAGGCT GGAGAGTCTG GCTCTTTTTT 
ATGCGTGGTA TCACACCTTT GCTGGAAAGG TGGCTTGGAA ATCTGCTTGC TAGACAATTT 
GAAGGTCGCC ATTCCAAAGG AGTTGCGAAA ACTGTGACAA AACAGCGAGT GGAGTCTCAC 
TTTGACTTGG AGCTCAGAGC TGCTGTTATG CATGACATAT TGGACATGAT GCCAGAGGGA 
ATCAAGCAGA ACAAAGCCAG AACCATCTTG CAGCACTTGA GTGAAGCTTG GAGATGCTGG 
AAAGCTAACA TTCCCTGGAA GGTACCAGGT CTTCCAACAC CCATTGAAAA CATGATTCTG 
CGATATGTAA AGGCCAAAGC GGACTGGTGG ACAAATACTG CGCATTACAA CAGAGAACGT 
ATTAGACGTG GTGCCACTGT CGACAAAACT GTGTGTAAGA AGAATCTGGG CCGTCTGACT 
CGTTTGTACT TGAAAGCAGA ACAAGAGAGA CAGCACAACT ACCTGAAGGA TGGGCCTTAC 
ATTACTGCAG AGGAGGCAGT TGCTGTGTAC ACTACCACTG TCCACTGGCT AGAGAGTAGG 
AGGTTTTCTC CAATTCCTTT TCCTCCTCTT TCCTACAAAC ATGATACAAA ACTACTCATT 
CTAGCACTTG AGAGACTGAA AGAAGCATAC AGTGTGAAGT CGCGTTTGAA TCAGTCTCAG 
AGGGAAGAAT TGGGTTTAAT CGAACAGGCG TATGACAACC CTCACGAAGC TCTGTCCAGA 
ATCAAGCGTC ACTTGCTCAC CCAAAGAGCT TTCAAGGAGG TTGGAATAGA GTTTATGGAT 
CTCTACAGCC ACCTTGTTCC TGTTTATGAC GTGGAACCAC TGGAAAAAAT TACTGATGCC 
TACCTGGATC AGTACCTGTG GTATGAAGCA GATAAAAGAA GGCTTTTCCC TCCATGGATC 
AAGCCTGCAG ATACAGAGCC ACCTCCCTTG TTGGTCTACA AGTGGTGCCA AGGCATAAAT 
AACCTGCAGG ATGTTTGGGA CACCACAGAA GGTGAATGCA ATGTGATGCT GGAGTCACGC 
TTTGAAAAGA TGTATGAAAA GATTGACTTG ACTCTGCTGA ATCGTCTGCT GCGTCTAATT 
GTTGATCACA ATATTGCTGA TTACATGACT GCAAAAAACA ACGTTGTGAT AAATTACAAG 
GATATGAACC ACACAAATTC CTATGGCATT ATTCGTGGTC TCCAGTTTGC TTCGTTTATT 
GTCCAGTACT ATGGTCTGGT GATGGATCTT CTGGTTCTTG GTTTACACAG AGCTAGTGAA 
ATGGCTGGGC CACCACAAAT GCCAAATGAC TTTCTAAGTT TCCAAGATGT GGCAACAGAA 
TCGGCCCATC CTATCCGCCT GTTTTGTCGA TACATTGACC GCATTCACAT TTTTTTCAGA 
TTTTCAGCTG ATGAAGCCAG AGACCTTATT CAGAGATATT TAACAGAACA TCCAGATCCC 
AATAATGAGA ATATTGTGGG CTACAACAAT AAAAAGTGTT GGCCAAGGGA TGCCAGAATG 
CGCCTCATGA AACATGATGT AAATCTGGGC CGTGCAGTGT TTTGGGATAT AAAAAATAGA 
CTGCCTCGTT CTGTCACCAC TGTGCAGTGG GAGAACAGTT TTGTGTCTGT GTACAGTAAA 
GACAATCCAA ACCTGCTGTT TAATATGTGC GGCTTTGAAT GCCGCATACT TCCCAAATGC 
CGCACAAGCT ATGAAGAGTT TACGCACAAG GATGGTGTCT GGAACCTGCA AAATGAAGTG 
ACCAAAGAAC GCACAGCTCA GTGCTTCTTA AGAGTTGATG ACGAGTCCAT GCAGAGATTT 
CACAACAGAG TCCGTCAAAT TCTTATGGCT TCCGGGTCCA CAACCTTTAC AAAAATTGTG 
AACAAGTGGA ATACAGCACT TATTGGCCTA ATGACTTATT TCCGTGAGGC TGTGGTCAAC 
ACCCAAGAAC TACTTGATCT GCTGGTGAAA TGTGAGAACA AAATCCAAAC AAGAATAAAG 
ATTGGGCTGA ACTCTAAAAT GCCAAGCAGG TTCCCGCCTG TTGTTTTTTA CACACCAAAG 
GAACTTGGAG GTTTGGGCAT GTTGTCCATG GGACATGTTT TAATTCCACA GTCTGACCTA 
AGGTGGTCTA AGCAGACAGA TGTTGGCATA ACCCATTTCC GTTCTGGCAT GAGTCACGAG 
GAGGATCAAC TGATTCCTAA TCTGTATCGT TACATTCAGC CTTGGGAAAG TGAGTTTATA 
GACTCTCAGA GAGTCTGGGC TGAGTATGCC TTAAAGAGGC AGGAGGCGAT TGCTCAAAAC 
AGACGCCTGA CGCTTGAAGA CTTAGAAGAC TCATGGGACA GGGGAATTCC ACGTATTAAT 
ACACTGTTTC AGAAAGATCG GCACACACTT GCTTATGACA AGGGTTGGAG AGTTCGCACA 
GACTTTAAAC AGTACCAGGT GCTGAAACAA AACCCGTTCT GGTGGACACA TCAGCGTCAT 
GATGGAAAGC TATGGAATCT GAACAACTAC CGCACAGATA TGATTCAGGC CCTTGGAGGG 
GTGGAGGGTA TACTGGAGCA CACGCTATTT AAAGGCACTT ACTTTCCCAC ATGGGAGGGT 
CTTTTCTGGG AAAAGGCCAG CGGCTTTGAA GAATCAATGA AATGGAAGAA ACTGACCAAT 
GCTCAGCGTT CTGGTTTAAA CCAGATACCA AACAGAAGAT TTACACTTTG GTGGTCTCCC 
ACAATCAACA GAGCCAACGT CTATGTTGGA TTCCAGGTGC AACTGGATTT GACTGGGATT 
TTCATGCATG GCAAGATTCC TACATTAAAA ATCTCATTGA TTCAGATATT CAGGGCCCAC 
TTGTGGCAAA AGATTCATGA AAGCATTGTC ATGGATTTAT GTCAGGTGTT TGATCAGGAG 
CTGGATGCTT TGGAGATTGA GACTGTGCAG AAAGAGACCA TCCACCCTAG GAAGTCCTAT 
AAGATGAACT CGTCCTGTGC TGATATTCTG CTCTTTGCCT CCTACAAATG GAATGTGTCT 
CGTCCCTCCT TGCTTGCTGA CTCAAAGGAT GTAATGGACA GCACAACCAC CCAGAAGTAC 
TGGATTGATA TACAGCTGAG ATGGGGTGAC TATGACTCCC ACGACATTGA AAGATATGCA 
CGTGCCAAAT TTTTGGATTA TACAACAGAT AACATGAGTA TCTATCCCTC ACCTACTGGT 
GTTCTTATTG CCATTGACCT TGCCTATAAC CTGCACAGTG CATATGGAAA CTGGTTCCCA 
GGGGGAAAGC CTCTAATACA GCAAGCAATG GCAAAGATAA TGAAGGCAAA CCCTGCCCTC 
TACGTGCTGC GGGAGCGCAT CCGTAAAGGA TTACAGCTTT ACTCTTCTGA GCCCACTGAG 
CCTTACCTGT CTTCTCAGAA CTATGGGGAA CTCTTCTCCA ATCAAATCAT CTGGTTTGTG 
GATGACACAA ATGTATACAG AGTCACCATC CACAAGACCT TTGAAGGTAA CCTGACAACC 
AAGCCAATCA ATGGAGCCAT TTTCATCTTT AATCCCAGGA CTGGCCAGCT CTTCTTAAAA 
ATTATTCATA CTTCTGTGTG GGCTGGACAG AAGAGATTGG GTCAGCTGGC CAAATGGAAA 
ACTGCAGAAG AAGTAGCTGC ATTGATTCGA TCTCTTCCAG TTGAGGAGCA GCCGAAACAA 
ATCATTGTCA CCAGGAAAGG CATGTTGGAT CCACTCGAGG TGCACTTGCT TGACTTCCCA 
AACATTGTCA TCAAAGGATC AGAATTACAG TTGCCTTTCC AGGCCTGTCT GAAGGTGGAG 
AAATTTGGTG ATCTGATCTT AAAGGCTACA GAGCCTCAGA TGGTTCTGTT CAATCTATAT 
GATGACTGGC TGAAGACGAT CTCTTCATAC ACGGCATTCT CTCGATTAAT TTTAATTCTA 
AGAGCCTTAC ATGTCAACAA TGACCGTGCA AAAGTGATCT TGAAACCTGA CAAGACCACT 
GTCACAGAGC CCCATCATAT CTGGCCCACA CTGACAGATG AGGAATGGAT TAAGGTTGAA 
GTTCAGCTAA AGGATTTGAT TTTAGCAGAT TATGGAAAGA AGAACAACGT AAATGTGGCC 
TCACTGACAC AGTCTGAAAT CAGAGACATC ATTCTGGGTA TGGAAATTTC TGCTCCATCC 
CAGCAAAGGC AGCAGATTGC AGAAATTGAA AAGCAAACAA AAGAACAGTC TCAGCTCACT 
GCTACTCAGA CGCGCACTGT CAACAAACAC GGAGATGAGA TTATCACATC TACCACCAGC 
AACTACGAAA CACAGACGTT CTCTTCAAAG ACTGAGTGGC GAGTCAGAGC AATATCTGCT 
GCCAACCTTC ACCTCCGAAC CAATCACATC TATGTATCGT CTGATGACAT TAAGGAAACT 
GGATACACCT ACATATTGCC CAAAAATGTG CTTAAGAAAT TCATTTGCAT CTCTGATCTT 
AGAGCACAGA TTGCTGGTTA CCTGTATGGT GTGAGCCCCC CAGATAATCC CCAAGTGAAG 
GAAATCCGTT GCATTGTTAT GGTGCCCCAG TGGGGAACTC ATCAAACTGT CCACCTGCCA 
AGCCAGCTGC CCCAACATGA ATACCTTAAG GAAATGGAGC CATTAGGATG GGTTCACACT 
CAGCCAAATG AGTCCCCACA GTTGTCACCT CAAGATGTCA CCACCCATGC CAAAGTAATG 
GCTGACAATC CTGCCTGGGA TGGAGAGAAA ACCATAATCA TTACTTGCAG CTTTACTCCA 
GGTTCATGCA CGTTAACCGC TTATAAACTG ACTCCCAGTG GTTATGAATG GGGACGGCAA 
AACACAGATA AAGGAAACAA TCCCAAGGGC TACTTGCCAT CGCATTATGA GAGGGTCCAG 
ATGCTGCTGT CTGATCGCTT CCTTGGGTTC TTCATGGTGC CAGCACAAGC GTCCTGGAAC 
TACAACTTCA TGGGTGTGAG ACATGACCCA AACATGAAAT ATGAGTTACA GCTGGCCAAC 
CCGAAGGAGT TTTACCATGA AGTACACCGG CCATCGCATT TCCTAAACTT TGCTCTGCTG 
CAGGAGGGTG AGGTGTATTC GGCTGACCGG GAAGATCTGT ATGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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prpf8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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