PRPF8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF8
Entrez Gene ID	507371
Full Name	pre-mRNA processing factor 8
General protein information	Preferred Names pre-mRNA processing factor 8 Names pre-mRNA-processing-splicing factor 8 PRP8 pre-mRNA processing factor 8 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105638.1	NP_001099108.1	pre-mRNA-processing-splicing factor 8
XM_024979809.1	XP_024835577.1	pre-mRNA-processing-splicing factor 8 isoform X1
XM_005220080.2	XP_005220137.1	pre-mRNA-processing-splicing factor 8 isoform X1
XM_005220080.3	XP_005220137.1	pre-mRNA-processing-splicing factor 8 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
Neurospora crassa	NCU07832	XP_962953.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Macaca mulatta (Rhesus monkey)	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Homo sapiens (human)	PRPF8	NP_006436.3
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa29516	NM_001105638.1 Latest version!	Bos taurus pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa29516	XM_005220080.3 Latest version!	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa29516	XM_024979809.1 Latest version!	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OBa29516	XM_005220080.2	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF8 ( NM_001105638.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001099108.1 Bos taurus pre-mRNA processing factor 8 (PRPF8), mRNA.

Price & Availability↑

CloneID	OBa29516
Clone ID Related Accession (Same CDS sequence)	NM_001105638.1 , XM_024979809.1 , XM_005220080.2 , XM_005220080.3
Accession Version	NM_001105638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526140800000
Organism	Bos taurus(cattle)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153223.1. On or before Dec 11, 2007 this sequence version replaced XR_027872.1, XR_027883.1. ##Evidence-Data-START## Transcript exon combination :: BC153223.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099108.1
CDS	212..7219
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	NM_001105638.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105638.1

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_024979809.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835577.1 Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

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CloneID	OBa29516
Clone ID Related Accession (Same CDS sequence)	NM_001105638.1 , XM_024979809.1 , XM_005220080.2 , XM_005220080.3
Accession Version	XM_024979809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	pre-mRNA-processing-splicing factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835577.1
CDS	93..7100
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_024979809.1
Organism	Bos taurus(cattle)
Definition	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979809.1

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_005220080.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220137.1 Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OBa29516
Clone ID Related Accession (Same CDS sequence)	NM_001105638.1 , XM_024979809.1 , XM_005220080.2 , XM_005220080.3
Accession Version	XM_005220080.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	pre-mRNA-processing-splicing factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005220080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220137.1
CDS	118..7125
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_005220080.2
Organism	Bos taurus(cattle)
Definition	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220080.2

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_005220080.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220137.1 Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OBa29516
Clone ID Related Accession (Same CDS sequence)	NM_001105638.1 , XM_024979809.1 , XM_005220080.2 , XM_005220080.3
Accession Version	XM_005220080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	pre-mRNA-processing-splicing factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220137.1
CDS	169..7176
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_005220080.3
Organism	Bos taurus(cattle)
Definition	Bos taurus pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220080.3

ORF Insert Sequence:

ATGGCCGGAG TATTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCTGGCCC TTTAGCCCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CCCGGAAATG GCAACAATTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCGCA GAAGGAAGAC 
ATGCCTCCAG AACATGTCAG GAAGATCATC CGAGACCATG GAGACATGAC CAACAGGAAG 
TTTCGGCATG ATAAAAGGGT GTACTTGGGG GCCCTCAAGT ACATGCCGCA TGCAGTCCTC 
AAGCTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATCCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGGG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CACCTTTGGA CTATGCTGAC 
AATATTCTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCATTAA GGGATAGCCG GAAGTATGTA 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACATTGCCTA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTCTTGAC AGACTTGGTT GATGACAATT ACTTCTATCT GTTTGATCTG 
AAGGCCTTTT TTACATCCAA GGCACTTAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGACATCAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACC GAGTACAAGA TTGCGTTTCC TTACCTGTAC 
AACAACCTGC CGCACCATGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGATTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATTTCC 
CATAGACACT CTGTCAAGAG CCAGGAACCA TTGCCAGATG ACGATGAGGA GTTTGAGCTC 
CCAGAATTTG TGGAGCCCTT TCTGAAGGAC ACACCCCTCT ACACAGACAA TACAGCCAAT 
GGCATCGCCC TGCTCTGGGC CCCCCGGCCC TTCAACCTAC GCTCTGGTCG CACTCGCCGG 
GCCCTAGACA TCCCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTCAA TGCCCTGAAG 
CACCGGCCCC CGAAAGCCCA AAAGAAGAGG TATCTATTCC GATCCTTCAA AGCCACCAAA 
TTCTTCCAGT CTACCAAGCT GGATTGGGTG GAGGTGGGGC TACAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGA AAGAACCTCA ACTATCTGCA CCTGGATTAC 
AACTTCAACC TTAAACCTGT TAAGACGCTC ACCACCAAGG AAAGGAAGAA ATCTCGTTTT 
GGAAATGCTT TCCATCTGTG TCGAGAAGTT CTGCGTCTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAT 
ATCTTCGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGCT ACAAATACAA GCTGATGCGG 
CAGATTCGTA TGTGCAAGGA CTTGAAGCAC CTTATCTATT ATCGCTTTAA CACGGGTCCT 
GTAGGGAAGG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTTTTC 
ATGCGTGGAA TCACCCCTTT GCTGGAGCGA TGGCTGGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGCCGAC ACTCAAAGGG GGTGGCCAAG ACGGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTTCGGGC CGCTGTGATG CATGACATCC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACGATCCTG CAGCACCTCA GTGAGGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CGATAGAGAA TATGATTCTT 
CGGTACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACCG CCCACTACAA CCGAGAGCGC 
ATCCGCCGCG GGGCCACCGT AGACAAGACT GTGTGCAAAA AGAACCTGGG CCGCCTGACT 
CGGCTCTACC TGAAGGCAGA GCAGGAGCGG CAGCACAACT ACCTAAAGGA CGGTCCTTAC 
ATCACAGCGG AGGAAGCGGT GGCGGTGTAT ACCACTACTG TGCATTGGCT GGAAAGCCGT 
AGATTCTCAC CTATCCCCTT CCCCCCGCTC TCCTACAAGC ATGACACCAA GTTGCTGATC 
TTGGCTTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CTCGATTAAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TATGACAACC CCCATGAGGC GCTGTCCCGC 
ATCAAGCGAC ACCTCCTTAC ACAGAGAGCC TTTAAAGAAG TGGGCATTGA GTTCATGGAC 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCAC TGGAGAAGAT CACTGATGCC 
TACCTGGACC AGTACCTGTG GTACGAAGCT GACAAGCGCC GCCTGTTCCC ACCCTGGATC 
AAGCCTGCAG ACACAGAACC ACCTCCTCTG CTTGTTTACA AATGGTGCCA AGGCATCAAT 
AACCTGCAGG ATGTGTGGGA GACAAGCGAG GGCGAGTGCA ATGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATTGACTTG ACCTTGCTCA ATAGGCTGCT GCGTCTAATC 
GTTGACCACA ATATAGCTGA CTACATGACA GCCAAGAACA ACGTCGTTAT CAATTACAAG 
GACATGAACC ATACAAATTC ATACGGGATC ATCAGAGGCC TGCAGTTTGC CTCTTTCATT 
GTACAGTACT ACGGCCTGGT GATGGACTTG CTTGTGTTGG GGTTGCACCG AGCCAGTGAG 
ATGGCCGGGC CACCTCAGAT GCCAAATGAT TTTCTCAGTT TCCAAGACAT AGCCACCGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATC CAGCGTTACC TGACAGAGCA CCCTGACCCC 
AACAACGAAA ACATCGTTGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA CGCCCGTATG 
CGCCTCATGA AGCATGATGT CAACCTAGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAATCCCA ACCTGCTATT CAACATGTGT GGTTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ACGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAACGAGGTG 
ACTAAAGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAAAT TCTGATGGCC TCTGGCTCCA CCACCTTCAC GAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGAGAGGC TGTGGTAAAC 
ACCCAGGAGC TCCTGGACTT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGAATCAAG 
ATTGGCCTCA ACTCCAAGAT GCCGAGTCGA TTCCCCCCTG TCGTTTTCTA CACCCCTAAG 
GAGTTGGGTG GACTTGGCAT GCTTTCAATG GGCCATGTGC TCATTCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTCC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TGATTCCCAA TTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGATTGA CCCTAGAAGA TCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAC 
ACTCTGTTCC AGAAGGACAG GCACACACTG GCTTATGATA AGGGCTGGCG TGTTCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AATCCTTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACCGACA TGATCCAGGC CCTGGGTGGT 
GTGGAAGGCA TTCTGGAACA CACCCTCTTC AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAAGCCAG TGGTTTTGAG GAGTCCATGA AGTGGAAGAA GCTCACCAAT 
GCCCAGCGAT CAGGATTGAA CCAGATTCCC AATCGTAGGT TCACCCTCTG GTGGTCCCCA 
ACCATCAACC GAGCCAACGT GTACGTGGGC TTTCAGGTGC AGCTGGACCT GACCGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAGATCGA GACAGTTCAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAATT CTTCCTGTGC AGATATCCTG CTGTTTGCAT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCCGA CTCTAAGGAT GTGATGGATA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TACAGTTGCG CTGGGGGGAC TACGATTCCC ATGACATCGA GCGTTATGCC 
CGGGCCAAGT TCTTGGACTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACTGGT 
GTGCTCATTG CCATTGACCT GGCATATAAC CTGCATAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA TCCTGCCTTG 
TATGTGTTAC GTGAACGGAT CCGTAAGGGG CTGCAGCTCT ATTCATCGGA GCCCACTGAG 
CCCTATCTGT CCTCTCAGAA CTACGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TTGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTTCTCAAG 
ATAATTCACA CATCTGTGTG GGCTGGACAG AAGCGTTTGG GACAGTTGGC TAAATGGAAG 
ACAGCTGAGG AGGTGGCTGC CCTAATCCGA TCTCTGCCCG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CTAGGAAAGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATTGTGA TTAAAGGATC AGAGCTCCAG CTCCCCTTCC AGGCTTGCCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAGCCACAGA TGGTTCTCTT TAACCTCTAT 
GACGACTGGC TCAAGACTAT CTCATCTTAC ACGGCCTTCT CCCGCCTCAT CCTGATCCTG 
CGCGCCCTGC ACGTAAACAA CGACCGCGCG AAGGTGATCC TGAAGCCGGA CAAGACCACG 
ATCACAGAGC CGCACCACAT CTGGCCCACT CTGACCGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCGCTGACAC AGTCAGAAAT TCGGGACATC ATCCTGGGGA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AGCAGATTGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCAACCCAGA CTCGCACTGT CAATAAGCAT GGTGACGAAA TAATCACCTC TACCACCAGC 
AACTACGAGA CCCAGACTTT CTCCTCTAAG ACTGAATGGA GGGTCAGGGC CATCTCTGCC 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGACAT CAAGGAGACT 
GGGTACACCT ATATCCTTCC CAAGAACGTG CTTAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGTTA CCTGTATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTACCACAG TGGGGCACTC ACCAGACTGT GCACCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAACG AGTCTCCTCA GCTGTCACCC CAGGATGTCA CCACCCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CGGATCGCTT CCTTGGCTTC TTTATGGTGC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTCCG GCATGATCCC AACATGAAGT ACGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGG CCCTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGCG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PRPF8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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