hmgcr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hmgcr
Entrez Gene ID	100380121
Full Name	3-hydroxy-3-methylglutaryl-CoA reductase
General protein information	Preferred Names 3-hydroxy-3-methylglutaryl-CoA reductase Names 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004910396.2	XP_004910453.1	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_004910396.4	XP_004910453.1	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_004910396.3	XP_004910453.1	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_002934844.3	XP_002934890.2	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_002934844.4	XP_002934890.2	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_002934844.5	XP_002934890.2	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
XM_018090768.1	XP_017946257.1	3-hydroxy-3-methylglutaryl-Coenzyme A reductase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B04642g	XP_451740.1
Homo sapiens (human)	HMGCR	NP_000850.1
Gallus gallus (chicken)	HMGCR	NP_989816.1
Caenorhabditis elegans (roundworm)	hmgr-1	NP_498626.2
Saccharomyces cerevisiae (baker's yeast)	HMG2	NP_013555.1
Pan troglodytes (chimpanzee)	HMGCR	XP_001148405.2
Bos taurus (cattle)	HMGCR	NP_001099083.1
Xenopus tropicalis (tropical clawed frog)	hmgcr	XP_004910453.1
Oryza sativa (rice)	Os08g0512700	NP_001062221.1
Oryza sativa (rice)	Os09g0492700	NP_001063541.1
Neurospora crassa	NCU00712	XP_964546.1
Magnaporthe oryzae (rice blast fungus)	MGG_08975	XP_003714014.1
Macaca mulatta (Rhesus monkey)	HMGCR	XP_002804463.1
Canis lupus familiaris (dog)	HMGCR	XP_536323.3
Drosophila melanogaster (fruit fly)	Hmgcr	NP_732900.1
Arabidopsis thaliana (thale cress)	HMG1	NP_177775.2
Mus musculus (house mouse)	Hmgcr	NP_032281.2
Rattus norvegicus (Norway rat)	Hmgcr	NP_037266.2
Danio rerio (zebrafish)	hmgcra	NP_001073446.1
Arabidopsis thaliana (thale cress)	HMG2	NP_179329.1
Eremothecium gossypii	AGOS_AER152W	NP_985010.2
Schizosaccharomyces pombe (fission yeast)	hmg1	NP_588235.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002288	XP_307890.5
Saccharomyces cerevisiae (baker's yeast)	HMG1	NP_013636.1

Gene Ontology

The following hmgcr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hmgcr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03353	XM_018090768.1 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03353	XM_004910396.4 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03353	XM_002934844.5 Latest version!	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa03353	XM_004910396.2	Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03353	XM_004910396.3	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03353	XM_002934844.3	Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03353	XM_002934844.4	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hmgcr ( XM_004910396.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910453.1 Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_004910396.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1434902400000
Organism	Xenopus (Silurana) tropicalis(Silurana) tropicalis (western clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_004910453.1

Translation

MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_004910396.2
Organism	Xenopus (Silurana) tropicalis(Silurana) tropicalis (western clawed frog)
Definition	Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910396.2

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_004910396.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910453.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_004910396.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910396.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910453.1
CDS	113..2764
Translation	MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_004910396.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910396.4

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_004910396.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910453.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

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CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_004910396.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910453.1
CDS	117..2768
Translation	MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_004910396.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910396.3

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_002934844.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934890.2 Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_002934844.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1434902400000
Organism	Xenopus (Silurana) tropicalis(Silurana) tropicalis (western clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_002934890.2

Translation

MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_002934844.3
Organism	Xenopus (Silurana) tropicalis(Silurana) tropicalis (western clawed frog)
Definition	Xenopus (Silurana) tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934844.3

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_002934844.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934890.2 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_002934844.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934844.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934890.2
CDS	158..2809
Translation	MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_002934844.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934844.4

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_002934844.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934890.2 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_002934844.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934844.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934890.2
CDS	121..2772
Translation	MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_002934844.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934844.5

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmgcr ( XM_018090768.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946257.1 Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X3, mRNA.

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CloneID	OXa03353
Clone ID Related Accession (Same CDS sequence)	XM_004910396.2 , XM_004910396.4 , XM_004910396.3 , XM_002934844.3 , XM_002934844.4 , XM_002934844.5 , XM_018090768.1
Accession Version	XM_018090768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946257.1
CDS	36..2687
Translation	MLSRLFRMHGQFVASHPWEVIVGTVTLTICMMSMNMFTGNDKICGWNYACPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKASALAKFALSSNSQDEVRDNIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFAFMTFFPACVSLVLELSRESREGRPIWQLSQFASVLEEEEDNKPNPVTQRVKMIMSLGLVLVHAHSRWISEPSSQNSTSIGDHEVSTMLDDMMPKRVEPSMPLWQFYLSRMVTMDVEQIITLGLALLLAVKYIFFEQTETESTFSLKNPIISPVAVQKKQAESCCRREPEQEKTVIVSSTEDASSKEEKEAVIRPLPLETSPKAKFMVGDSSPVELSPEEKNTSFDLPEEPRPLDECVRILKNPDKGAQYLTDAEVISLVNAKHIPAYKLETMMESPERGVAVRRQMLADKLPQPSALQSLPYKNYNYSLVMGACCENVIGYMPIPVGVAGPLFLNNKEYQVPMATTEGCLVASTNRGCRAIMLGGGARSRVLADGMTRGPVVRLPTACDAAEVKAWLDSMEGFKVIKDAFDSTSRFARLGRLQTCVAGRNLYIRFQSKTGDAMGMNMISKGTEKALSRLQEEFPELHVLAVSGNYCTDKKPAAINWIEGRGKSVVCEAIIPARVVREVLKSSTEALVEVNINKNLIGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITIMEATGPMNEDLYISCTMPSIEIGTVGGGTNLAPQQACLQMLGVQGASTEAPGKNACQLAQIVCGTVMAGELSLMAALAAGHLVKSHMVHNRSKMNLQDLPGTCTKKAA

Target ORF information:

RefSeq Version	XM_018090768.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 3-hydroxy-3-methylglutaryl-CoA reductase (hmgcr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090768.1

ORF Insert Sequence:

ATGCTGTCTA GGCTATTCCG CATGCATGGC CAGTTTGTGG CCTCTCACCC CTGGGAAGTC 
ATCGTGGGGA CTGTGACTCT CACGATCTGC ATGATGTCAA TGAACATGTT CACTGGGAAT 
GACAAAATCT GTGGATGGAA TTATGCTTGC CCCAAATTTG AGGAAGATGT TCTGAGCAGC 
GACATCATCA TCCTGACAAT CACAAGATGC ATCGCCATCC TTTATATTTA TTTCCAGTTC 
CAGAACCTCA GGCAGCTCGG CTCAAAATAC ATTTTAGGGA TTGCTGGGCT TTTCACCATC 
TTCTCAAGCT TTGTCTTCAG CACAGTGGTC ATTCACTTCC TAGATAAAGA ACTAACTGGC 
CTTAATGAAG CTCTTCCATT TTTCCTGCTT CTGATTGATC TTTCCAAGGC TAGTGCTTTA 
GCCAAATTTG CTCTAAGTTC CAACTCACAG GATGAAGTCC GAGACAATAT TGCCCGTGGC 
ATGGCAATTT TAGGACCTAC CTTTACCCTT GATGCACTTG TTGAATGTCT TGTGATTGGA 
GTTGGCACAA TGTCAGGTGT GCGGCAGTTA GAAATCATGT GTTGCTTTGG CTGCATGTCT 
GTCCTTGCCA ACTATTTTGC CTTCATGACC TTCTTTCCAG CTTGTGTTTC ACTGGTGTTA 
GAGCTTTCTA GGGAGAGCCG CGAGGGCCGT CCAATCTGGC AGCTCAGTCA GTTTGCTAGT 
GTACTAGAAG AAGAGGAGGA TAATAAACCA AATCCCGTTA CTCAGAGAGT GAAAATGATT 
ATGTCCTTGG GATTGGTCCT TGTTCATGCT CATAGTCGAT GGATAAGTGA ACCCTCATCC 
CAAAACAGCA CTTCAATTGG TGACCATGAA GTTTCCACCA TGCTGGATGA TATGATGCCA 
AAGAGAGTTG AGCCCAGCAT GCCCCTCTGG CAGTTCTATC TTTCCAGGAT GGTGACCATG 
GATGTAGAGC AGATCATTAC TCTTGGGCTT GCTCTTCTCC TTGCCGTGAA GTACATATTC 
TTTGAGCAAA CTGAGACAGA ATCAACCTTC TCTCTAAAGA ACCCCATAAT CTCCCCCGTA 
GCTGTGCAGA AGAAGCAAGC AGAGAGTTGC TGCAGACGGG AACCTGAACA AGAAAAAACA 
GTCATTGTTT CCTCAACAGA AGACGCTTCA TCAAAGGAGG AAAAAGAGGC AGTTATAAGA 
CCATTACCCC TTGAGACATC ACCCAAAGCC AAGTTTATGG TGGGAGATTC TTCTCCAGTA 
GAATTGTCCC CAGAAGAAAA AAATACATCA TTTGACCTGC CGGAGGAACC CCGTCCACTT 
GATGAATGTG TTCGTATCCT CAAGAATCCA GATAAAGGTG CCCAGTATCT GACTGATGCA 
GAAGTCATCA GCCTAGTGAA TGCCAAGCAT ATCCCAGCCT ACAAACTGGA AACTATGATG 
GAATCTCCTG AGAGGGGCGT GGCTGTTCGT CGGCAAATGT TGGCTGATAA GCTGCCCCAA 
CCATCAGCAC TGCAGAGCTT GCCCTATAAG AACTACAACT ACTCTTTAGT AATGGGCGCT 
TGTTGTGAGA ATGTCATCGG CTACATGCCC ATCCCTGTAG GTGTAGCTGG ACCTCTCTTT 
CTGAACAACA AAGAGTATCA GGTGCCCATG GCAACAACTG AAGGGTGTCT TGTGGCTAGC 
ACCAACAGAG GTTGCAGAGC CATAATGCTG GGAGGAGGTG CCAGAAGTCG TGTTCTAGCC 
GATGGAATGA CTCGTGGACC AGTTGTTAGG TTACCAACTG CCTGTGATGC AGCAGAAGTA 
AAGGCCTGGC TTGACAGCAT GGAGGGATTT AAAGTCATAA AGGATGCTTT TGATAGCACA 
AGCAGATTTG CTCGACTTGG GAGACTACAA ACCTGCGTTG CTGGCAGGAA CCTGTACATT 
CGTTTCCAGT CAAAAACGGG AGATGCCATG GGAATGAACA TGATTTCAAA AGGCACTGAG 
AAAGCTCTCT CTAGACTACA AGAAGAGTTC CCTGAACTCC ATGTCTTGGC CGTCAGTGGC 
AACTACTGTA CAGATAAGAA GCCTGCTGCT ATTAACTGGA TAGAGGGTAG AGGAAAGTCT 
GTGGTGTGTG AAGCAATCAT TCCAGCCAGA GTTGTACGGG AGGTTCTAAA GAGCTCAACA 
GAAGCATTAG TTGAAGTAAA TATCAACAAG AATCTGATTG GTTCTGCAAT GGCGGGAAGC 
ATCGGCGGCT ACAATGCCCA TGCCGCAAAC ATTGTGACTG CCATTTACAT TGCTTGCGGA 
CAGGATGCTG CTCAGAATGT TGGGAGCTCC AATTGCATCA CAATAATGGA GGCCACTGGT 
CCTATGAATG AAGATTTGTA CATCAGCTGC ACAATGCCCT CTATTGAAAT TGGAACTGTA 
GGAGGTGGAA CCAACTTGGC TCCCCAGCAA GCTTGCTTAC AGATGCTGGG GGTGCAAGGA 
GCAAGCACAG AGGCACCAGG GAAGAATGCA TGCCAACTCG CTCAGATCGT CTGTGGCACA 
GTTATGGCAG GGGAACTGTC ACTAATGGCA GCTTTGGCAG CGGGTCATCT AGTCAAGAGT 
CACATGGTCC ACAACAGGTC AAAAATGAAC CTTCAAGACC TGCCAGGCAC ATGCACAAAG 
AAAGCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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hmgcr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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