topbp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	topbp1
Entrez Gene ID	100380063
Full Name	DNA topoisomerase II binding protein 1
Synonyms	Xmus101,cut5,mus101,top2bp1
General protein information	Preferred Names DNA topoisomerase II binding protein 1 Names DNA topoisomerase 2-binding protein 1 topoisomerase (DNA) II binding protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937745.4	XP_002937791.3	DNA topoisomerase 2-binding protein 1 isoform X1
XM_018094989.1	XP_017950478.1	DNA topoisomerase 2-binding protein 1 isoform X3
XM_002937745.3	XP_002937791.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_004915369.3	XP_004915426.2	DNA topoisomerase 2-binding protein 1 isoform X2
XM_004915368.3	XP_004915425.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_004915368.4	XP_004915425.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_004915369.2	XP_004915426.1	DNA topoisomerase 2-binding protein 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TOPBP1	NP_001193695.1
Rattus norvegicus (Norway rat)	Topbp1	XP_236578.6
Homo sapiens (human)	TOPBP1	NP_008958.2
Danio rerio (zebrafish)	topbp1	NP_001186667.1
Xenopus tropicalis (tropical clawed frog)	topbp1	XP_004915426.1
Macaca mulatta (Rhesus monkey)	TOPBP1	XP_001112636.2
Mus musculus (house mouse)	Topbp1	NP_795953.2
Gallus gallus (chicken)	TOPBP1	XP_418794.1
Pan troglodytes (chimpanzee)	TOPBP1	XP_516761.3
Canis lupus familiaris (dog)	TOPBP1	XP_005634503.1

Gene Ontology

The following topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45147	XM_018094989.1 Latest version!	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa11878	XM_002937745.3 Latest version!	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa11878	XM_004915368.3 Latest version!	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa11879	XM_004915369.2 Latest version!	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60131	XM_002937745.4 Latest version!	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60131	XM_004915368.4 Latest version!	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60132	XM_004915369.3 Latest version!	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

topbp1 ( XM_018094989.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950478.1 Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa45147
Clone ID Related Accession (Same CDS sequence)	XM_018094989.1
Accession Version	XM_018094989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAT ATTTGTGCCC AATATTTCGT GGTTGCACTA TCTGTGTCAC AGGATTAAAC 
AGTTTAGACA GAAAGGAGGT GCAACGTCTT ACAACCCTCC ATGGAGGAGA GTACACAGGG 
CAACTCAAAA TGAATGAATC TACACACCTT ATTGTACAAG AGGCTAAAGG CCAGAAATAT 
GAGTGTGCCA GAAAGTGGAA TGTACACTGT GTCTCCGTTC AATGGTTTTT CGACAGTCTG 
GACAAAGGAT TCTGTCAGGA TGAAACAATG TATAAAATTG AGCCTGCTAC TAGTTTAAAA 
TCTGTGCCAG ATACATCCAC TCCTACAGGT GACAATAAGA AACCAGATAG TCGGGCCATT 
TATGATGTCA GCCAAATTTC AAATATAAGT ACAAGCTGCG TTAACGAATC GGCTTTTAAT 
TCTGTGATGA CCAGCAGACT GGATCCTCCA GCAGATGCCC TTGAGAACTT GGATATCAGT 
TCATTACAAG CTCCAGAAGA CCTTCTTGAT GGCTGCCGAA TATATTTGTG CGGGTTTGGG 
GGAAGAAAGT TGGACAAGTT GAGAAGGCTA ATCAACAGTG GTGGGGGTGT GCGATTTAGT 
CAGCTTACCG CAGATGTAAC CCATATTATT GTTGGAGAAA CTGATGAAGA ACTAAAGCAG 
TTTTTGAGCA AAACTCAACA CAGGCCTTAT GTGTTAACTG TAAAATGGCT ACTGGAAAGC 
TTTTCAAAAG GACATCTGCA ACCTGAGAAC ATATATTCCC ATTCAAGCTA TCAACAAACT 
GAGCTTTCTT CACCAGTTCA GCCTGCCTTT AATTTAACTG CTCAAAAGAT CAGTAGTACA 
CGAGGGCCTC TAAACCACAC TCACAACCAA CAGGCAGATG AGGACCTGCT GTCTCAGTAC 
ACAGAAAATA ACTCCACTTT AATTGAGGAC GAACATCCTA AAATCTCTAA TACCAACAGT 
ATTTCTCAAA TGAGCATGCA TGAAGATATG ACATCTTGTA CCAGCCAAAG TGGTTTGGCG 
GATACATCCA CCATAATTGA AGGAGGCTTA TTCAGCCGTA AACAATTCTT GGTGCTGGGC 
TTTTTGGAGG AAGATGAGGC ATGCATCATA GACATAATAA AAAAGAATGC TGGCAAGGTG 
CTTTCATCTC AGAAAAGAGC CATTGCTGAT TATGCTGTAG TTCCCTTACT GGGTTGTGAG 
GTGGAATCTA CAGTTGGAGA GGTTGTGACC AATGCTTGGC TGGGCATGTG TATAGAACAA 
GAGAAGCTCC TAGACCCACA TTCAAATGCA CTTTTTACAC CTGTGCCATG TTTGGAAGGT 
AGTCGACCGC TGCAAGAGTG CGTGCTTTCT GTCAGCCAGT TTATGGGCGC TGAAAGGGAT 
TCATTAGTTT ACTTGGCCGG TTTGCTTGGA GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG 
GCCAATCCAA AAAAGGACAT GTTTGCCAGT ACACACTTAG TTCTTAAAGA TGCTGAAGGG 
TCAAAATACG AAGCAGCCAA AAAGTGGAAT TTGCCAGCAG TGACAATGAA GTGGCTTTTG 
CAGTGTGCTA GAACTGGTAA AAAAGCAGAT GAACACCATT ATCTTGTTGA CAGTGTTCCT 
GAAGAAGATG AGAGTTTTAT AAGTCAGACA TACAAACCAC AAGTATCCAG GCGATCAATG 
GATGCACCAT GCCATCAAGA AAACCATCCA CAAGCCCTAA AGAAAGCTGC AGTTACCCCA 
CTTGACATGA ACCGGTTTCA GAGCAAAGCA TTTCAGTCTG TTATTTCTCA GCATAACAAG 
AACCCACAAA CACCTGGAGG GGAAAGCACA GTTCTTCAGA GAGAACCATC TTTGCATCTT 
GACACACCAT CAAAATTTCT TTCCAAAGAC AAACTTTTCA AACCCTCCTT TGATGTTAAG 
GATGCTCTTG CAGCTCTGGA AACACCCGGA GGTCCCAACC AAAAAAGCAG GACACAGAGC 
ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT TTGCAGCTGG CTATTGCAAA CAGCACACGT 
CAAACTGCTG CGGTCACTGC AAGCCCTCAG CTAAAGGCAG CAGAACAAAA AGAGCAGTTA 
GAGAACTCCA AGGTACTGCA TAATGTTGTT ATATGTGTGA GCAAGAAGCT AATTAAAAAA 
CAAGGTGAAC TGAATGGCAT TGCTGCTTCA CTTGGAGCAG AATACAGATG GTGCTTCGAT 
GAAAGCGTGA CCCATTTTAT CTACCACGGA CGACAAAACG ACATGAGTCG AGAATACAAA 
TCTGTGAAAG AACGAAGTGG CATCTGCATA GTTTCTGAAC ACTGGCTATT AGCTTGTTCA 
GAACAACAGA AGAGAGTACC TGAGGCTCTC TATCCCCATA CCTACAATCC TAAAATGAGC 
TTGGATATCA GTGGTGTACA AGATGGATCT CACACAGCCA GCAAATTCTC AGCAGACACC 
TCTCTTCAGC TGGATGAGAA TTGTGAATTA AGGCTTCAGC AGAATGATGA ACTTGGGGAG 
ACATCCGATG ACCAGGTTAA AAAGGAAGCT GATAATAGAG ATCCTCAAAA CCCTTTAAAA 
GAAGTGAAAG GAGCTTTAAC TCAGACTTTA GAGATGAGAG AAAATTTCCA GCGGCAGCTA 
CAAGAATTAA TGTCTGCAAC TTCAGTGGTA AAGCCTCAAG GGCCCCGGGG CTCCTTGACT 
AGAGTTGGCT TTGATAATTC TCCTTGTACA CCTGAAGGGG CACGTTCTAC ACGTAATGGA 
AGAAGTAGAG TTTTGGAAGC ACTAAGACAG TCTCGGCAGG CTACAACAGA CCTCAATACA 
GAGCCATCAC AGAACGAGCA AATAATTTGG GATGATCCTA CTGCCAGAGA AGAAAGAGCA 
AAGCTGGTCA GCAATCTACA GTGGCCCGAC AGTCCCTCCC AGTATTCAGA ACAGCTTCAG 
CTTAATATGA CTGATGCTGG TGGAAATTAT GCAGCAGTAA AGGAATCTTT AACAGATACT 
GAAATAGCAG AATTGGAAGC TTGTGAATTT GAGCATAAAT CATTCACTAG AGCTCCTATG 
ACAGAGAATG ATTTGCAGTC TCCAAACAAA CTGGATCAAC TCACTCCTAC CCCACAAGCT 
CCAAGCATTG CATTTCCACT TGCCAACCCT CCAGTGGCAC CACAACCTAA AGAAAAGCCT 
GTGAAACCAT TTTCAGAGGA GAAAACCATA AAGGAGCGTC GCTTCCAGCT ATCATCCTTG 
GACCCTCAAG AACGAATTGA TTACTCACAG CTTATTGAGG AAATAGGGGG GGTGGTGATA 
GAAAAACAGT GTTTTGATCC AAGCTGCACA CATATCATTG TGGGGCATCC TCTTCGGAAT 
GAGAAATATT TGGCCTCAAT GGCTGCAGGA AAGTGGGTAC TACACCGGTC ATATCTGGAA 
GCCTGCAGAG CTGCAAAACA ATTCATACAG GAGGATGACT ATGAATGGGG AAGCAAGTCT 
ATACTGAGTG CTGTGACCAA CATAAATCCC CAGCAGAGGA TGCTGGCAGA GGCTGCAATG 
AGATGGAGGA AAAAGCTGCA AGGAATAAAG CAAAATATGG GCATTGCTGA GGGTGCATTC 
AGCGGCTGGA AAGTCATTTT AAATGTTGAC CAAACAAAGG AACCTGGTTT CAAACGTTTG 
CTTCAGTCAG GAGGTGCAAA GGTATTTGCT GGCCATTCTT CTGCTCTGTT TAAAGAAGCC 
AACCACCTCT TTGCTGACTT CAGCAAACTG AAACCTGATG AGCCCAAGGT GAATGTGGCC 
GAGGCTGCAG CACAAGGAGT AAACTGCCTA AAGCCAGAGT ATATTGCCGA CTACCTCATG 
AAGGAGCCTC CTCCACCAGT CAACAATTAC TGCCTCCCTG ATGCTCTGCC ATATCTTCAG 
ATCACAGTGA CTGGGCTTTC TCGCAAAAGG AAAAACTCTG GAGACATCTC TGATGTTAAG 
AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950478.1
CDS	110..4087
Translation	MKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESTVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKEQLENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSFTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_018094989.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094989.1

ORF Insert Sequence:

ATGAAAGAAT ATTTGTGCCC AATATTTCGT GGTTGCACTA TCTGTGTCAC AGGATTAAAC 
AGTTTAGACA GAAAGGAGGT GCAACGTCTT ACAACCCTCC ATGGAGGAGA GTACACAGGG 
CAACTCAAAA TGAATGAATC TACACACCTT ATTGTACAAG AGGCTAAAGG CCAGAAATAT 
GAGTGTGCCA GAAAGTGGAA TGTACACTGT GTCTCCGTTC AATGGTTTTT CGACAGTCTG 
GACAAAGGAT TCTGTCAGGA TGAAACAATG TATAAAATTG AGCCTGCTAC TAGTTTAAAA 
TCTGTGCCAG ATACATCCAC TCCTACAGGT GACAATAAGA AACCAGATAG TCGGGCCATT 
TATGATGTCA GCCAAATTTC AAATATAAGT ACAAGCTGCG TTAACGAATC GGCTTTTAAT 
TCTGTGATGA CCAGCAGACT GGATCCTCCA GCAGATGCCC TTGAGAACTT GGATATCAGT 
TCATTACAAG CTCCAGAAGA CCTTCTTGAT GGCTGCCGAA TATATTTGTG CGGGTTTGGG 
GGAAGAAAGT TGGACAAGTT GAGAAGGCTA ATCAACAGTG GTGGGGGTGT GCGATTTAGT 
CAGCTTACCG CAGATGTAAC CCATATTATT GTTGGAGAAA CTGATGAAGA ACTAAAGCAG 
TTTTTGAGCA AAACTCAACA CAGGCCTTAT GTGTTAACTG TAAAATGGCT ACTGGAAAGC 
TTTTCAAAAG GACATCTGCA ACCTGAGAAC ATATATTCCC ATTCAAGCTA TCAACAAACT 
GAGCTTTCTT CACCAGTTCA GCCTGCCTTT AATTTAACTG CTCAAAAGAT CAGTAGTACA 
CGAGGGCCTC TAAACCACAC TCACAACCAA CAGGCAGATG AGGACCTGCT GTCTCAGTAC 
ACAGAAAATA ACTCCACTTT AATTGAGGAC GAACATCCTA AAATCTCTAA TACCAACAGT 
ATTTCTCAAA TGAGCATGCA TGAAGATATG ACATCTTGTA CCAGCCAAAG TGGTTTGGCG 
GATACATCCA CCATAATTGA AGGAGGCTTA TTCAGCCGTA AACAATTCTT GGTGCTGGGC 
TTTTTGGAGG AAGATGAGGC ATGCATCATA GACATAATAA AAAAGAATGC TGGCAAGGTG 
CTTTCATCTC AGAAAAGAGC CATTGCTGAT TATGCTGTAG TTCCCTTACT GGGTTGTGAG 
GTGGAATCTA CAGTTGGAGA GGTTGTGACC AATGCTTGGC TGGGCATGTG TATAGAACAA 
GAGAAGCTCC TAGACCCACA TTCAAATGCA CTTTTTACAC CTGTGCCATG TTTGGAAGGT 
AGTCGACCGC TGCAAGAGTG CGTGCTTTCT GTCAGCCAGT TTATGGGCGC TGAAAGGGAT 
TCATTAGTTT ACTTGGCCGG TTTGCTTGGA GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG 
GCCAATCCAA AAAAGGACAT GTTTGCCAGT ACACACTTAG TTCTTAAAGA TGCTGAAGGG 
TCAAAATACG AAGCAGCCAA AAAGTGGAAT TTGCCAGCAG TGACAATGAA GTGGCTTTTG 
CAGTGTGCTA GAACTGGTAA AAAAGCAGAT GAACACCATT ATCTTGTTGA CAGTGTTCCT 
GAAGAAGATG AGAGTTTTAT AAGTCAGACA TACAAACCAC AAGTATCCAG GCGATCAATG 
GATGCACCAT GCCATCAAGA AAACCATCCA CAAGCCCTAA AGAAAGCTGC AGTTACCCCA 
CTTGACATGA ACCGGTTTCA GAGCAAAGCA TTTCAGTCTG TTATTTCTCA GCATAACAAG 
AACCCACAAA CACCTGGAGG GGAAAGCACA GTTCTTCAGA GAGAACCATC TTTGCATCTT 
GACACACCAT CAAAATTTCT TTCCAAAGAC AAACTTTTCA AACCCTCCTT TGATGTTAAG 
GATGCTCTTG CAGCTCTGGA AACACCCGGA GGTCCCAACC AAAAAAGCAG GACACAGAGC 
ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT TTGCAGCTGG CTATTGCAAA CAGCACACGT 
CAAACTGCTG CGGTCACTGC AAGCCCTCAG CTAAAGGCAG CAGAACAAAA AGAGCAGTTA 
GAGAACTCCA AGGTACTGCA TAATGTTGTT ATATGTGTGA GCAAGAAGCT AATTAAAAAA 
CAAGGTGAAC TGAATGGCAT TGCTGCTTCA CTTGGAGCAG AATACAGATG GTGCTTCGAT 
GAAAGCGTGA CCCATTTTAT CTACCACGGA CGACAAAACG ACATGAGTCG AGAATACAAA 
TCTGTGAAAG AACGAAGTGG CATCTGCATA GTTTCTGAAC ACTGGCTATT AGCTTGTTCA 
GAACAACAGA AGAGAGTACC TGAGGCTCTC TATCCCCATA CCTACAATCC TAAAATGAGC 
TTGGATATCA GTGGTGTACA AGATGGATCT CACACAGCCA GCAAATTCTC AGCAGACACC 
TCTCTTCAGC TGGATGAGAA TTGTGAATTA AGGCTTCAGC AGAATGATGA ACTTGGGGAG 
ACATCCGATG ACCAGGTTAA AAAGGAAGCT GATAATAGAG ATCCTCAAAA CCCTTTAAAA 
GAAGTGAAAG GAGCTTTAAC TCAGACTTTA GAGATGAGAG AAAATTTCCA GCGGCAGCTA 
CAAGAATTAA TGTCTGCAAC TTCAGTGGTA AAGCCTCAAG GGCCCCGGGG CTCCTTGACT 
AGAGTTGGCT TTGATAATTC TCCTTGTACA CCTGAAGGGG CACGTTCTAC ACGTAATGGA 
AGAAGTAGAG TTTTGGAAGC ACTAAGACAG TCTCGGCAGG CTACAACAGA CCTCAATACA 
GAGCCATCAC AGAACGAGCA AATAATTTGG GATGATCCTA CTGCCAGAGA AGAAAGAGCA 
AAGCTGGTCA GCAATCTACA GTGGCCCGAC AGTCCCTCCC AGTATTCAGA ACAGCTTCAG 
CTTAATATGA CTGATGCTGG TGGAAATTAT GCAGCAGTAA AGGAATCTTT AACAGATACT 
GAAATAGCAG AATTGGAAGC TTGTGAATTT GAGCATAAAT CATTCACTAG AGCTCCTATG 
ACAGAGAATG ATTTGCAGTC TCCAAACAAA CTGGATCAAC TCACTCCTAC CCCACAAGCT 
CCAAGCATTG CATTTCCACT TGCCAACCCT CCAGTGGCAC CACAACCTAA AGAAAAGCCT 
GTGAAACCAT TTTCAGAGGA GAAAACCATA AAGGAGCGTC GCTTCCAGCT ATCATCCTTG 
GACCCTCAAG AACGAATTGA TTACTCACAG CTTATTGAGG AAATAGGGGG GGTGGTGATA 
GAAAAACAGT GTTTTGATCC AAGCTGCACA CATATCATTG TGGGGCATCC TCTTCGGAAT 
GAGAAATATT TGGCCTCAAT GGCTGCAGGA AAGTGGGTAC TACACCGGTC ATATCTGGAA 
GCCTGCAGAG CTGCAAAACA ATTCATACAG GAGGATGACT ATGAATGGGG AAGCAAGTCT 
ATACTGAGTG CTGTGACCAA CATAAATCCC CAGCAGAGGA TGCTGGCAGA GGCTGCAATG 
AGATGGAGGA AAAAGCTGCA AGGAATAAAG CAAAATATGG GCATTGCTGA GGGTGCATTC 
AGCGGCTGGA AAGTCATTTT AAATGTTGAC CAAACAAAGG AACCTGGTTT CAAACGTTTG 
CTTCAGTCAG GAGGTGCAAA GGTATTTGCT GGCCATTCTT CTGCTCTGTT TAAAGAAGCC 
AACCACCTCT TTGCTGACTT CAGCAAACTG AAACCTGATG AGCCCAAGGT GAATGTGGCC 
GAGGCTGCAG CACAAGGAGT AAACTGCCTA AAGCCAGAGT ATATTGCCGA CTACCTCATG 
AAGGAGCCTC CTCCACCAGT CAACAATTAC TGCCTCCCTG ATGCTCTGCC ATATCTTCAG 
ATCACAGTGA CTGGGCTTTC TCGCAAAAGG AAAAACTCTG GAGACATCTC TGATGTTAAG 
AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_002937745.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937791.2 Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa11878
Clone ID Related Accession (Same CDS sequence)	XM_002937745.3 , XM_004915368.3
Accession Version	XM_002937745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937791.2
CDS	140..4687
Translation	MARSENEPFCVKFIKSSENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESTVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKEQLENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSFTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_002937745.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937745.3

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_004915368.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915425.1 Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

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CloneID	OXa11878
Clone ID Related Accession (Same CDS sequence)	XM_002937745.3 , XM_004915368.3
Accession Version	XM_004915368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915425.1
CDS	98..4645
Translation	MARSENEPFCVKFIKSSENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESTVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKEQLENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSFTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_004915368.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915368.3

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CATTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_004915369.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915426.1 Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

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CloneID	OXa11879
Clone ID Related Accession (Same CDS sequence)	XM_004915369.2
Accession Version	XM_004915369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004915369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA 
TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT 
GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA 
CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT 
TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT 
CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC 
ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG 
CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT 
AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG 
ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG 
CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT 
GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT 
CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT 
GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT 
CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA 
GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG 
CATAAATCAT TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG 
GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA 
GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG 
GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT 
ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT 
ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG 
TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG 
GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG 
CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA 
AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA 
ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC 
CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA 
CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG 
CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC 
CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA 
AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915426.1
CDS	140..4684
Translation	MARSENEPFCVKFIKSSENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESTVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKELENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSFTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_004915369.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II binding protein 1 (topbp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915369.2

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTTC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTTTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAGGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACCCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCACA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA 
TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT 
GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA 
CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT 
TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT 
CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC 
ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG 
CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT 
AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG 
ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG 
CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT 
GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT 
CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT 
GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT 
CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA 
GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG 
CATAAATCAT TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG 
GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA 
GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG 
GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT 
ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT 
ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG 
TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG 
GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG 
CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA 
AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA 
ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC 
CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA 
CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG 
CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC 
CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA 
AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_002937745.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937791.3 Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X1, mRNA.

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CloneID	OXa60131
Clone ID Related Accession (Same CDS sequence)	XM_002937745.4 , XM_004915368.4
Accession Version	XM_002937745.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937745.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937791.3
CDS	138..4685
Translation	MARSENEPFCVKFIKSPENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESKVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKEQLENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSVTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_002937745.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937745.4

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_004915368.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915425.2 Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa60131
Clone ID Related Accession (Same CDS sequence)	XM_002937745.4 , XM_004915368.4
Accession Version	XM_004915368.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915368.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915425.2
CDS	246..4793
Translation	MARSENEPFCVKFIKSPENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESKVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKEQLENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSVTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_004915368.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915368.4

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGCAGTTA GAGAACTCCA AGGTACTGCA TAATGTTGTT 
ATATGTGTGA GCAAGAAGCT AATTAAAAAA CAAGGTGAAC TGAATGGCAT TGCTGCTTCA 
CTTGGAGCAG AATACAGATG GTGCTTCGAT GAAAGCGTGA CCCATTTTAT CTACCACGGA 
CGACAAAACG ACATGAGTCG AGAATACAAA TCTGTGAAAG AACGAAGTGG CATCTGCATA 
GTTTCTGAAC ACTGGCTATT AGCTTGTTCA GAACAACAGA AGAGAGTACC TGAGGCTCTC 
TATCCCCATA CCTACAATCC TAAAATGAGC TTGGATATCA GTGGTGTACA AGATGGATCT 
CACACAGCCA GCAAATTCTC AGCAGACACC TCTCTTCAGC TGGATGAGAA TTGTGAATTA 
AGGCTTCAGC AGAATGATGA ACTTGGGGAG ACATCCGATG ACCAGGTTAA AAAGGAAGCT 
GATAATAGAG ATCCTCAAAA CCCTTTAAAA GAAGTGAAAG GAGCTTTAAC TCAGACTTTA 
GAGATGAGAG AAAATTTCCA GCGGCAGCTA CAAGAATTAA TGTCTGCAAC TTCAGTGGTA 
AAGCCTCAAG GGCCCCGGGG CTCCTTGACT AGAGTTGGCT TTGATAATTC TCCTTGTACA 
CCTGAAGGGG CACGTTCTAC ACGTAATGGA AGAAGTAGAG TTTTGGAAGC ACTAAGACAG 
TCTCGGCAGG CTACAACAGA CCTCAATACA GAGCCATCAC AGAACGAGCA AATAATTTGG 
GATGATCCTA CTGCCAGAGA AGAAAGAGCA AAGCTGGTCA GCAATCTACA GTGGCCCGAC 
AGTCCCTCCC AGTATTCAGA ACAGCTTCAG CTTAATATGA CTGATGCTGG TGGAAATTAT 
GCAGCAGTAA AGGAATCTTT AACAGATACT GAAATAGCAG AATTGGAAGC TTGTGAATTT 
GAGCATAAAT CAGTCACTAG AGCTCCTATG ACAGAGAATG ATTTGCAGTC TCCAAACAAA 
CTGGATCAAC TCACTCCTAC CCCACAAGCT CCAAGCATTG CATTTCCACT TGCCAACCCT 
CCAGTGGCAC CACAACCTAA AGAAAAGCCT GTGAAACCAT TTTCAGAGGA GAAAACCATA 
AAGGAGCGTC GCTTCCAGCT ATCATCCTTG GACCCTCAAG AACGAATTGA TTACTCACAG 
CTTATTGAGG AAATAGGGGG GGTGGTGATA GAAAAACAGT GTTTTGATCC AAGCTGCACA 
CATATCATTG TGGGGCATCC TCTTCGGAAT GAGAAATATT TGGCCTCAAT GGCTGCAGGA 
AAGTGGGTAC TACACCGGTC ATATCTGGAA GCCTGCAGAG CTGCAAAACA ATTCATACAG 
GAGGATGACT ATGAATGGGG AAGCAAGTCT ATACTGAGTG CTGTGACCAA CATAAATCCC 
CAGCAGAGGA TGCTGGCAGA GGCTGCAATG AGATGGAGGA AAAAGCTGCA AGGAATAAAG 
CAAAATATGG GCATTGCTGA GGGTGCATTC AGCGGCTGGA AAGTCATTTT AAATGTTGAC 
CAAACAAAGG AACCTGGTTT CAAACGTTTG CTTCAGTCAG GAGGTGCAAA GGTATTTGCT 
GGCCATTCTT CTGCTCTGTT TAAAGAAGCC AACCACCTCT TTGCTGACTT CAGCAAACTG 
AAACCTGATG AGCCCAAGGT GAATGTGGCC GAGGCTGCAG CACAAGGAGT AAACTGCCTA 
AAGCCAGAGT ATATTGCCGA CTACCTCATG AAGGAGCCTC CTCCACCAGT CAACAATTAC 
TGCCTCCCTG ATGCTCTGCC ATATCTTCAG ATCACAGTGA CTGGGCTTTC TCGCAAAAGG 
AAAAACTCTG GAGACATCTC TGATGTTAAG AGATCACGGC TTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topbp1 ( XM_004915369.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915426.2 Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X3, mRNA.

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CloneID	OXa60132
Clone ID Related Accession (Same CDS sequence)	XM_004915369.3
Accession Version	XM_004915369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA 
TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT 
GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA 
CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT 
TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT 
CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC 
ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG 
CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT 
AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG 
ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG 
CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT 
GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT 
CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT 
GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT 
CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA 
GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG 
CATAAATCAG TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG 
GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA 
GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG 
GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT 
ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT 
ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG 
TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG 
GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG 
CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA 
AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA 
ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC 
CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA 
CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG 
CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC 
CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA 
AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915426.2
CDS	133..4677
Translation	MARSENEPFCVKFIKSPENSEYFFKAYEAIKQIQSDESLQLTEESEALLLKEKDKSLYICDPFSGAVFNHLKKLGCRIVGPQVVIFCMEHQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHNYVQLMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKALWDQSNQQIIRYCDVNMKEYLCPIFRGCTICVTGLNSLDRKEVQRLTTLHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCVSVQWFFDSLDKGFCQDETMYKIEPATSLKSVPDTSTPTGDNKKPDSRAIYDVSQISNISTSCVNESAFNSVMTSRLDPPADALENLDISSLQAPEDLLDGCRIYLCGFGGRKLDKLRRLINSGGGVRFSQLTADVTHIIVGETDEELKQFLSKTQHRPYVLTVKWLLESFSKGHLQPENIYSHSSYQQTELSSPVQPAFNLTAQKISSTRGPLNHTHNQQADEDLLSQYTENNSTLIEDEHPKISNTNSISQMSMHEDMTSCTSQSGLADTSTIIEGGLFSRKQFLVLGFLEEDEACIIDIIKKNAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPCLEGSRPLQECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKDMFASTHLVLKDAEGSKYEAAKKWNLPAVTMKWLLQCARTGKKADEHHYLVDSVPEEDESFISQTYKPQVSRRSMDAPCHQENHPQALKKAAVTPLDMNRFQSKAFQSVISQHNKNPQTPGGESKVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKSRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEQKELENSKVLHNVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGICIVSEHWLLACSEQQKRVPEALYPHTYNPKMSLDISGVQDGSHTASKFSADTSLQLDENCELRLQQNDELGETSDDQVKKEADNRDPQNPLKEVKGALTQTLEMRENFQRQLQELMSATSVVKPQGPRGSLTRVGFDNSPCTPEGARSTRNGRSRVLEALRQSRQATTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQLNMTDAGGNYAAVKESLTDTEIAELEACEFEHKSVTRAPMTENDLQSPNKLDQLTPTPQAPSIAFPLANPPVAPQPKEKPVKPFSEEKTIKERRFQLSSLDPQERIDYSQLIEEIGGVVIEKQCFDPSCTHIIVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKQFIQEDDYEWGSKSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSALFKEANHLFADFSKLKPDEPKVNVAEAAAQGVNCLKPEYIADYLMKEPPPPVNNYCLPDALPYLQITVTGLSRKRKNSGDISDVKRSRLY

Target ORF information:

RefSeq Version	XM_004915369.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase II binding protein 1 (topbp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915369.3

ORF Insert Sequence:

ATGGCTAGGA GTGAAAATGA GCCATTTTGT GTGAAATTTA TCAAGTCTCC TGAAAATTCT 
GAATACTTCT TTAAAGCTTA TGAGGCAATC AAGCAGATTC AGTCTGATGA ATCTCTTCAG 
CTAACTGAAG AAAGTGAGGC ACTTCTCTTG AAAGAAAAAG ACAAATCTCT CTACATTTGT 
GATCCTTTTA GTGGTGCTGT CTTTAACCAT CTGAAAAAGC TTGGCTGTAG GATAGTTGGG 
CCACAAGTTG TCATTTTTTG TATGGAGCAT CAAAGACGTG TCCCTCGTGC TGAATATCCT 
GTCTATAATA TGGCAATGGC TGATGTGACA ATTTCCTGTA CCAGTCTCGA TAAAGAAACT 
AGGGAAGATG TGCATAATTA TGTACAACTC ATGGGAGGCT GTGTATACAG GGATCTTAAT 
GTTTCTGTTA CACACCTAAT TGCTGGTGAA GTGGGCAGTA AAAAATACCT GGTGGCTGCA 
AGCCTAGAGA AGCCCATTCT TCTTCCGTCT TGGGTGAAAG CATTATGGGA TCAATCAAAT 
CAGCAGATTA TTCGATACTG TGATGTAAAC ATGAAAGAAT ATTTGTGCCC AATATTTCGT 
GGTTGCACTA TCTGTGTCAC AGGATTAAAC AGTTTAGACA GAAAGGAGGT GCAACGTCTT 
ACAACCCTCC ATGGAGGAGA GTACACAGGG CAACTCAAAA TGAATGAATC TACACACCTT 
ATTGTACAAG AGGCTAAAGG CCAGAAATAT GAGTGTGCCA GAAAGTGGAA TGTACACTGT 
GTCTCCGTTC AATGGTTCTT CGACAGTCTG GACAAAGGAT TCTGTCAGGA TGAAACAATG 
TATAAAATTG AGCCTGCTAC TAGTTTAAAA TCTGTGCCAG ATACATCCAC TCCTACAGGT 
GACAATAAGA AACCAGATAG TCGGGCCATT TATGATGTCA GCCAAATTTC AAATATAAGT 
ACAAGCTGCG TTAACGAATC GGCTTTTAAT TCTGTGATGA CCAGCAGACT GGATCCTCCA 
GCAGATGCCC TTGAGAACTT GGATATCAGT TCATTACAAG CTCCAGAAGA CCTTCTTGAT 
GGCTGCCGAA TATATTTGTG CGGGTTTGGG GGAAGAAAGT TGGACAAGTT GAGAAGGCTA 
ATCAACAGTG GTGGGGGTGT GCGATTTAGT CAGCTTACCG CAGATGTAAC CCATATTATT 
GTTGGAGAAA CTGATGAAGA ACTAAAGCAG TTTTTGAGCA AAACTCAACA CAGGCCTTAT 
GTGTTAACTG TAAAATGGCT ACTGGAAAGC TTTTCAAAAG GACATCTGCA ACCTGAGAAC 
ATATATTCCC ATTCAAGCTA TCAACAAACT GAGCTTTCTT CACCAGTTCA GCCTGCCTTT 
AATTTAACTG CTCAAAAGAT CAGTAGTACA CGAGGGCCTC TAAACCACAC TCACAACCAA 
CAGGCAGATG AGGACCTGCT GTCTCAGTAC ACAGAAAATA ACTCCACTTT AATTGAGGAC 
GAACATCCTA AAATCTCTAA TACCAACAGT ATTTCTCAAA TGAGCATGCA TGAAGATATG 
ACATCTTGTA CCAGCCAAAG TGGTTTGGCG GATACATCCA CCATAATTGA AGGAGGCTTA 
TTCAGCCGTA AACAATTCTT GGTGCTGGGC TTTTTGGAGG AAGATGAGGC ATGCATCATA 
GACATAATAA AAAAGAATGC TGGCAAGGTG CTTTCATCTC AGAAAAGAGC CATTGCTGAT 
TATGCTGTAG TTCCCTTACT GGGTTGTGAG GTGGAATCTA CAGTTGGAGA GGTTGTGACC 
AATGCTTGGC TGGGCATGTG TATAGAACAA GAGAAGCTCC TAGACCCACA TTCAAATGCA 
CTTTTTACAC CTGTGCCATG TTTGGAAGGT AGTCGACCGC TGCAAGAGTG CGTGCTTTCT 
GTCAGCCAGT TTATGGGCGC TGAAAGGGAT TCATTAGTTT ACTTGGCCGG TTTGCTTGGA 
GCAAAAGTTC AAGAGTTTTT TGTGCGGAAG GCCAATCCAA AAAAAGACAT GTTTGCCAGT 
ACACACTTAG TTCTTAAAGA TGCTGAAGGG TCAAAATACG AAGCAGCCAA AAAGTGGAAT 
TTGCCAGCAG TGACAATGAA GTGGCTTTTG CAGTGTGCTA GAACTGGTAA AAAAGCAGAT 
GAACACCATT ATCTTGTTGA CAGTGTTCCT GAAGAAGATG AGAGTTTTAT AAGTCAGACA 
TACAAACCAC AAGTATCCAG GCGATCAATG GATGCACCAT GCCATCAAGA AAACCATCCA 
CAAGCCCTAA AGAAAGCTGC AGTTACTCCA CTTGACATGA ACCGGTTTCA GAGCAAAGCA 
TTTCAGTCTG TTATTTCTCA GCATAACAAG AACCCACAAA CACCTGGAGG GGAAAGCAAA 
GTTCTTCAGA GAGAACCATC TTTGCATCTT GACACACCAT CAAAATTTCT TTCCAAAGAC 
AAACTTTTCA AACCCTCCTT TGATGTTAAG GATGCTCTTG CAGCTCTGGA AACACCCGGA 
GGTCCCAACC AAAAAAGCAG GACACAGAGC ACTCCCCTGT CTGAAGTTAT TGGTAGGAAT 
TTGCAGCTGG CTATTGCAAA CAGCACACGT CAAACTGCTG CGGTCACTGC AAGCCCTCAG 
CTAAAGGCAG CAGAACAAAA AGAGTTAGAG AACTCCAAGG TACTGCATAA TGTTGTTATA 
TGTGTGAGCA AGAAGCTAAT TAAAAAACAA GGTGAACTGA ATGGCATTGC TGCTTCACTT 
GGAGCAGAAT ACAGATGGTG CTTCGATGAA AGCGTGACCC ATTTTATCTA CCACGGACGA 
CAAAACGACA TGAGTCGAGA ATACAAATCT GTGAAAGAAC GAAGTGGCAT CTGCATAGTT 
TCTGAACACT GGCTATTAGC TTGTTCAGAA CAACAGAAGA GAGTACCTGA GGCTCTCTAT 
CCCCATACCT ACAATCCTAA AATGAGCTTG GATATCAGTG GTGTACAAGA TGGATCTCAC 
ACAGCCAGCA AATTCTCAGC AGACACCTCT CTTCAGCTGG ATGAGAATTG TGAATTAAGG 
CTTCAGCAGA ATGATGAACT TGGGGAGACA TCCGATGACC AGGTTAAAAA GGAAGCTGAT 
AATAGAGATC CTCAAAACCC TTTAAAAGAA GTGAAAGGAG CTTTAACTCA GACTTTAGAG 
ATGAGAGAAA ATTTCCAGCG GCAGCTACAA GAATTAATGT CTGCAACTTC AGTGGTAAAG 
CCTCAAGGGC CCCGGGGCTC CTTGACTAGA GTTGGCTTTG ATAATTCTCC TTGTACACCT 
GAAGGGGCAC GTTCTACACG TAATGGAAGA AGTAGAGTTT TGGAAGCACT AAGACAGTCT 
CGGCAGGCTA CAACAGACCT CAATACAGAG CCATCACAGA ACGAGCAAAT AATTTGGGAT 
GATCCTACTG CCAGAGAAGA AAGAGCAAAG CTGGTCAGCA ATCTACAGTG GCCCGACAGT 
CCCTCCCAGT ATTCAGAACA GCTTCAGCTT AATATGACTG ATGCTGGTGG AAATTATGCA 
GCAGTAAAGG AATCTTTAAC AGATACTGAA ATAGCAGAAT TGGAAGCTTG TGAATTTGAG 
CATAAATCAG TCACTAGAGC TCCTATGACA GAGAATGATT TGCAGTCTCC AAACAAACTG 
GATCAACTCA CTCCTACCCC ACAAGCTCCA AGCATTGCAT TTCCACTTGC CAACCCTCCA 
GTGGCACCAC AACCTAAAGA AAAGCCTGTG AAACCATTTT CAGAGGAGAA AACCATAAAG 
GAGCGTCGCT TCCAGCTATC ATCCTTGGAC CCTCAAGAAC GAATTGATTA CTCACAGCTT 
ATTGAGGAAA TAGGGGGGGT GGTGATAGAA AAACAGTGTT TTGATCCAAG CTGCACACAT 
ATCATTGTGG GGCATCCTCT TCGGAATGAG AAATATTTGG CCTCAATGGC TGCAGGAAAG 
TGGGTACTAC ACCGGTCATA TCTGGAAGCC TGCAGAGCTG CAAAACAATT CATACAGGAG 
GATGACTATG AATGGGGAAG CAAGTCTATA CTGAGTGCTG TGACCAACAT AAATCCCCAG 
CAGAGGATGC TGGCAGAGGC TGCAATGAGA TGGAGGAAAA AGCTGCAAGG AATAAAGCAA 
AATATGGGCA TTGCTGAGGG TGCATTCAGC GGCTGGAAAG TCATTTTAAA TGTTGACCAA 
ACAAAGGAAC CTGGTTTCAA ACGTTTGCTT CAGTCAGGAG GTGCAAAGGT ATTTGCTGGC 
CATTCTTCTG CTCTGTTTAA AGAAGCCAAC CACCTCTTTG CTGACTTCAG CAAACTGAAA 
CCTGATGAGC CCAAGGTGAA TGTGGCCGAG GCTGCAGCAC AAGGAGTAAA CTGCCTAAAG 
CCAGAGTATA TTGCCGACTA CCTCATGAAG GAGCCTCCTC CACCAGTCAA CAATTACTGC 
CTCCCTGATG CTCTGCCATA TCTTCAGATC ACAGTGACTG GGCTTTCTCG CAAAAGGAAA 
AACTCTGGAG ACATCTCTGA TGTTAAGAGA TCACGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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topbp1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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