TOPBP1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPBP1
Entrez Gene ID	420694
Full Name	topoisomerase (DNA) II binding protein 1
General protein information	Preferred Names topoisomerase (DNA) II binding protein 1 Names DNA topoisomerase 2-binding protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015281749.1	XP_015137235.1	DNA topoisomerase 2-binding protein 1
XM_015281750.1	XP_015137236.1	DNA topoisomerase 2-binding protein 1
XM_015281749.2	XP_015137235.1	DNA topoisomerase 2-binding protein 1
XM_015281750.2	XP_015137236.1	DNA topoisomerase 2-binding protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TOPBP1	NP_001193695.1
Rattus norvegicus (Norway rat)	Topbp1	XP_236578.6
Homo sapiens (human)	TOPBP1	NP_008958.2
Danio rerio (zebrafish)	topbp1	NP_001186667.1
Xenopus tropicalis (tropical clawed frog)	topbp1	XP_004915426.1
Macaca mulatta (Rhesus monkey)	TOPBP1	XP_001112636.2
Mus musculus (house mouse)	Topbp1	NP_795953.2
Gallus gallus (chicken)	TOPBP1	XP_418794.1
Pan troglodytes (chimpanzee)	TOPBP1	XP_516761.3
Canis lupus familiaris (dog)	TOPBP1	XP_005634503.1

Gene Ontology

The following TOPBP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPBP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa04441	XM_015281750.2 Latest version!	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa04441	XM_015281749.2 Latest version!	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OGa04441	XM_015281749.1	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa04441	XM_015281750.1	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPBP1 ( XM_015281749.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137235.1 Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa04441
Clone ID Related Accession (Same CDS sequence)	XM_015281749.1 , XM_015281750.1 , XM_015281749.2 , XM_015281750.2
Accession Version	XM_015281749.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137235.1
CDS	139..4698
Translation	MKGSKEVFLVKFVKSSGSSEYFLKALESIKEFQSEEHLQILEEEAALNIKENDKSLYICDPFTGVVFNHLKKLGCRIVGPQVVLYCMQSQRCVPRAEYPVYNMTMADVTISCTTLDKDVREEVHKYVQMMGGRVYRDLNMSVTHLIAGEVGSKKYLVAASLKKPVLLPSWVKTLWDKSQQRMMRYTDVNMEDYACPVFLGCTICVTGLSSSDRKEVQRLTAEHGGQYSGQLKMNECTHLIVQEPKGQKYECAKKWNVHCVPVQWFSDSIEKGFCQDETMYKIESGSKLSSTPSTSTPTSHASKLDTHSLSDVSCISNVNLSRVNETACSSAMSSRLDPPPDELENLDISSFQAPEDLLDGCRVYLCGFSGRKLDKMRRLINCGGGVRFNQLNEDVTHVILGENSDELKHFLDKTLHRPHIVTAKWLLESFNKGYLCPVEQYIPQNYQLLANTILEQPDAKLVLPKNNSMSKKKAVNVVKLQKAAEDDFLSQYVSNDSTLVEAEKLASGSFNDVTHVTVQEENHSSVCNGSLGEPSALDEGLFARKRFLLLGFGEEDESCIADLIKEHAGKTVPLQSRIIADYAVVPLLGCSVESTVGDVVTNTWLVTCVEQQLLLDPQSNPLFTPVPVMEGVTPLVDCVLSFSQFTGAERDSLVYLAGLLGARVQEFFVRKANAKKGMFASTHLVVKEPDGSKYEAAKKWNLPAVTVAWLLQSARTGKRADESKFLVEHADAEDESSITKLSKTPPTTKFPDSPERPTYLREAGKKPAVTPLDINRFQSKAFQAVISHHIGKATTSPPPRGLLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGLGQKNRKLSTPLSEVIGRNLKLALANSTRNTEALTASPQLKAAQPEVEEEPKPLAGVVICVSKKLTKKQSELNAIAASLGADYRWCFDETVTHFIYKGGQNDSNKEYRSVKERGIHIVSEHWLLECAQEYKRLPESLFPHTYNPKMSLDISAVQDFRPSSSSELPPTGKPAEENEIIAVDEDDAEDDLTTDQIKETAGTGEEHNGISESKGVLTQALEMRENFQRQLQEIMSATSIVKPQGQRGSLSRNSFDSSPATPDSARSVRNGRSRALEALRQSRQAFTDINTEPSQSEQIIWDDPTAREERARLVSNFQWPNSPSQYTEQAQSNVNRNTDESTFKGSIADAEIAGIVVPEAGDGDTIEGLKNPVCRDPETPIKDHLIPTPQAPSIAFPLANPPVAPQPKEKAVTEDEKVDEEPEKCRKFQLSSLNPQERFDYCHLIEELGGIVLEKQCFDPSCTHIVVGHPLRNEKFLASMAAGKWVLHRSYLEACRGAGCFVQEEDYEWGSDSILNVLPGINVNQKKLALAAMRWRKRIHKGRQETGITEGAFSGWKVILNVDHTKEAGFRRLLQSGGAKVFSGHSVSFFKEATHLFADFSKLKPDDTRVNIAEAAEQGVNCLKPEYIADFLIQDPPPPMESYCLPEAERCFQNNKERGTGLSQKRKAPGEMSRVKRSRMH

Target ORF information:

RefSeq Version	XM_015281749.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281749.1

ORF Insert Sequence:

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_015281750.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137236.1 Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa04441
Clone ID Related Accession (Same CDS sequence)	XM_015281749.1 , XM_015281750.1 , XM_015281749.2 , XM_015281750.2
Accession Version	XM_015281750.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137236.1
CDS	135..4694
Translation	MKGSKEVFLVKFVKSSGSSEYFLKALESIKEFQSEEHLQILEEEAALNIKENDKSLYICDPFTGVVFNHLKKLGCRIVGPQVVLYCMQSQRCVPRAEYPVYNMTMADVTISCTTLDKDVREEVHKYVQMMGGRVYRDLNMSVTHLIAGEVGSKKYLVAASLKKPVLLPSWVKTLWDKSQQRMMRYTDVNMEDYACPVFLGCTICVTGLSSSDRKEVQRLTAEHGGQYSGQLKMNECTHLIVQEPKGQKYECAKKWNVHCVPVQWFSDSIEKGFCQDETMYKIESGSKLSSTPSTSTPTSHASKLDTHSLSDVSCISNVNLSRVNETACSSAMSSRLDPPPDELENLDISSFQAPEDLLDGCRVYLCGFSGRKLDKMRRLINCGGGVRFNQLNEDVTHVILGENSDELKHFLDKTLHRPHIVTAKWLLESFNKGYLCPVEQYIPQNYQLLANTILEQPDAKLVLPKNNSMSKKKAVNVVKLQKAAEDDFLSQYVSNDSTLVEAEKLASGSFNDVTHVTVQEENHSSVCNGSLGEPSALDEGLFARKRFLLLGFGEEDESCIADLIKEHAGKTVPLQSRIIADYAVVPLLGCSVESTVGDVVTNTWLVTCVEQQLLLDPQSNPLFTPVPVMEGVTPLVDCVLSFSQFTGAERDSLVYLAGLLGARVQEFFVRKANAKKGMFASTHLVVKEPDGSKYEAAKKWNLPAVTVAWLLQSARTGKRADESKFLVEHADAEDESSITKLSKTPPTTKFPDSPERPTYLREAGKKPAVTPLDINRFQSKAFQAVISHHIGKATTSPPPRGLLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGLGQKNRKLSTPLSEVIGRNLKLALANSTRNTEALTASPQLKAAQPEVEEEPKPLAGVVICVSKKLTKKQSELNAIAASLGADYRWCFDETVTHFIYKGGQNDSNKEYRSVKERGIHIVSEHWLLECAQEYKRLPESLFPHTYNPKMSLDISAVQDFRPSSSSELPPTGKPAEENEIIAVDEDDAEDDLTTDQIKETAGTGEEHNGISESKGVLTQALEMRENFQRQLQEIMSATSIVKPQGQRGSLSRNSFDSSPATPDSARSVRNGRSRALEALRQSRQAFTDINTEPSQSEQIIWDDPTAREERARLVSNFQWPNSPSQYTEQAQSNVNRNTDESTFKGSIADAEIAGIVVPEAGDGDTIEGLKNPVCRDPETPIKDHLIPTPQAPSIAFPLANPPVAPQPKEKAVTEDEKVDEEPEKCRKFQLSSLNPQERFDYCHLIEELGGIVLEKQCFDPSCTHIVVGHPLRNEKFLASMAAGKWVLHRSYLEACRGAGCFVQEEDYEWGSDSILNVLPGINVNQKKLALAAMRWRKRIHKGRQETGITEGAFSGWKVILNVDHTKEAGFRRLLQSGGAKVFSGHSVSFFKEATHLFADFSKLKPDDTRVNIAEAAEQGVNCLKPEYIADFLIQDPPPPMESYCLPEAERCFQNNKERGTGLSQKRKAPGEMSRVKRSRMH

Target ORF information:

RefSeq Version	XM_015281750.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281750.1

ORF Insert Sequence:

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_015281749.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137235.1 Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

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CloneID	OGa04441
Clone ID Related Accession (Same CDS sequence)	XM_015281749.1 , XM_015281750.1 , XM_015281749.2 , XM_015281750.2
Accession Version	XM_015281749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281749.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137235.1
CDS	145..4704
Translation	MKGSKEVFLVKFVKSSGSSEYFLKALESIKEFQSEEHLQILEEEAALNIKENDKSLYICDPFTGVVFNHLKKLGCRIVGPQVVLYCMQSQRCVPRAEYPVYNMTMADVTISCTTLDKDVREEVHKYVQMMGGRVYRDLNMSVTHLIAGEVGSKKYLVAASLKKPVLLPSWVKTLWDKSQQRMMRYTDVNMEDYACPVFLGCTICVTGLSSSDRKEVQRLTAEHGGQYSGQLKMNECTHLIVQEPKGQKYECAKKWNVHCVPVQWFSDSIEKGFCQDETMYKIESGSKLSSTPSTSTPTSHASKLDTHSLSDVSCISNVNLSRVNETACSSAMSSRLDPPPDELENLDISSFQAPEDLLDGCRVYLCGFSGRKLDKMRRLINCGGGVRFNQLNEDVTHVILGENSDELKHFLDKTLHRPHIVTAKWLLESFNKGYLCPVEQYIPQNYQLLANTILEQPDAKLVLPKNNSMSKKKAVNVVKLQKAAEDDFLSQYVSNDSTLVEAEKLASGSFNDVTHVTVQEENHSSVCNGSLGEPSALDEGLFARKRFLLLGFGEEDESCIADLIKEHAGKTVPLQSRIIADYAVVPLLGCSVESTVGDVVTNTWLVTCVEQQLLLDPQSNPLFTPVPVMEGVTPLVDCVLSFSQFTGAERDSLVYLAGLLGARVQEFFVRKANAKKGMFASTHLVVKEPDGSKYEAAKKWNLPAVTVAWLLQSARTGKRADESKFLVEHADAEDESSITKLSKTPPTTKFPDSPERPTYLREAGKKPAVTPLDINRFQSKAFQAVISHHIGKATTSPPPRGLLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGLGQKNRKLSTPLSEVIGRNLKLALANSTRNTEALTASPQLKAAQPEVEEEPKPLAGVVICVSKKLTKKQSELNAIAASLGADYRWCFDETVTHFIYKGGQNDSNKEYRSVKERGIHIVSEHWLLECAQEYKRLPESLFPHTYNPKMSLDISAVQDFRPSSSSELPPTGKPAEENEIIAVDEDDAEDDLTTDQIKETAGTGEEHNGISESKGVLTQALEMRENFQRQLQEIMSATSIVKPQGQRGSLSRNSFDSSPATPDSARSVRNGRSRALEALRQSRQAFTDINTEPSQSEQIIWDDPTAREERARLVSNFQWPNSPSQYTEQAQSNVNRNTDESTFKGSIADAEIAGIVVPEAGDGDTIEGLKNPVCRDPETPIKDHLIPTPQAPSIAFPLANPPVAPQPKEKAVTEDEKVDEEPEKCRKFQLSSLNPQERFDYCHLIEELGGIVLEKQCFDPSCTHIVVGHPLRNEKFLASMAAGKWVLHRSYLEACRGAGCFVQEEDYEWGSDSILNVLPGINVNQKKLALAAMRWRKRIHKGRQETGITEGAFSGWKVILNVDHTKEAGFRRLLQSGGAKVFSGHSVSFFKEATHLFADFSKLKPDDTRVNIAEAAEQGVNCLKPEYIADFLIQDPPPPMESYCLPEAERCFQNNKERGTGLSQKRKAPGEMSRVKRSRMH

Target ORF information:

RefSeq Version	XM_015281749.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281749.2

ORF Insert Sequence:

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPBP1 ( XM_015281750.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137236.1 Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

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CloneID	OGa04441
Clone ID Related Accession (Same CDS sequence)	XM_015281749.1 , XM_015281750.1 , XM_015281749.2 , XM_015281750.2
Accession Version	XM_015281750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-binding protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137236.1
CDS	129..4688
Translation	MKGSKEVFLVKFVKSSGSSEYFLKALESIKEFQSEEHLQILEEEAALNIKENDKSLYICDPFTGVVFNHLKKLGCRIVGPQVVLYCMQSQRCVPRAEYPVYNMTMADVTISCTTLDKDVREEVHKYVQMMGGRVYRDLNMSVTHLIAGEVGSKKYLVAASLKKPVLLPSWVKTLWDKSQQRMMRYTDVNMEDYACPVFLGCTICVTGLSSSDRKEVQRLTAEHGGQYSGQLKMNECTHLIVQEPKGQKYECAKKWNVHCVPVQWFSDSIEKGFCQDETMYKIESGSKLSSTPSTSTPTSHASKLDTHSLSDVSCISNVNLSRVNETACSSAMSSRLDPPPDELENLDISSFQAPEDLLDGCRVYLCGFSGRKLDKMRRLINCGGGVRFNQLNEDVTHVILGENSDELKHFLDKTLHRPHIVTAKWLLESFNKGYLCPVEQYIPQNYQLLANTILEQPDAKLVLPKNNSMSKKKAVNVVKLQKAAEDDFLSQYVSNDSTLVEAEKLASGSFNDVTHVTVQEENHSSVCNGSLGEPSALDEGLFARKRFLLLGFGEEDESCIADLIKEHAGKTVPLQSRIIADYAVVPLLGCSVESTVGDVVTNTWLVTCVEQQLLLDPQSNPLFTPVPVMEGVTPLVDCVLSFSQFTGAERDSLVYLAGLLGARVQEFFVRKANAKKGMFASTHLVVKEPDGSKYEAAKKWNLPAVTVAWLLQSARTGKRADESKFLVEHADAEDESSITKLSKTPPTTKFPDSPERPTYLREAGKKPAVTPLDINRFQSKAFQAVISHHIGKATTSPPPRGLLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGLGQKNRKLSTPLSEVIGRNLKLALANSTRNTEALTASPQLKAAQPEVEEEPKPLAGVVICVSKKLTKKQSELNAIAASLGADYRWCFDETVTHFIYKGGQNDSNKEYRSVKERGIHIVSEHWLLECAQEYKRLPESLFPHTYNPKMSLDISAVQDFRPSSSSELPPTGKPAEENEIIAVDEDDAEDDLTTDQIKETAGTGEEHNGISESKGVLTQALEMRENFQRQLQEIMSATSIVKPQGQRGSLSRNSFDSSPATPDSARSVRNGRSRALEALRQSRQAFTDINTEPSQSEQIIWDDPTAREERARLVSNFQWPNSPSQYTEQAQSNVNRNTDESTFKGSIADAEIAGIVVPEAGDGDTIEGLKNPVCRDPETPIKDHLIPTPQAPSIAFPLANPPVAPQPKEKAVTEDEKVDEEPEKCRKFQLSSLNPQERFDYCHLIEELGGIVLEKQCFDPSCTHIVVGHPLRNEKFLASMAAGKWVLHRSYLEACRGAGCFVQEEDYEWGSDSILNVLPGINVNQKKLALAAMRWRKRIHKGRQETGITEGAFSGWKVILNVDHTKEAGFRRLLQSGGAKVFSGHSVSFFKEATHLFADFSKLKPDDTRVNIAEAAEQGVNCLKPEYIADFLIQDPPPPMESYCLPEAERCFQNNKERGTGLSQKRKAPGEMSRVKRSRMH

Target ORF information:

RefSeq Version	XM_015281750.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II binding protein 1 (TOPBP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281750.2

ORF Insert Sequence:

ATGAAAGGCA GCAAGGAGGT GTTCTTGGTG AAATTCGTAA AATCTTCTGG GAGCTCGGAG 
TATTTCTTGA AAGCTTTGGA GTCTATAAAA GAATTTCAGT CAGAAGAACA TCTCCAGATT 
CTTGAAGAAG AAGCAGCACT CAATATAAAA GAAAATGATA AATCCCTTTA CATCTGTGAT 
CCTTTTACAG GTGTTGTTTT CAATCATCTC AAAAAGCTTG GTTGTAGAAT TGTTGGACCA 
CAGGTAGTCC TGTACTGCAT GCAGTCCCAG AGATGTGTCC CAAGAGCTGA GTATCCTGTG 
TACAACATGA CCATGGCTGA TGTAACGATA TCGTGTACCA CCTTAGATAA AGATGTTCGG 
GAGGAAGTTC ATAAGTATGT ACAGATGATG GGTGGACGTG TGTACAGAGA CCTCAACATG 
TCAGTAACTC ATCTTATAGC TGGAGAAGTT GGCAGCAAGA AATACTTAGT AGCTGCTTCT 
CTGAAAAAAC CTGTTTTGCT TCCTTCTTGG GTTAAGACGC TATGGGATAA ATCTCAGCAA 
AGAATGATGA GATACACTGA TGTTAACATG GAAGACTACG CTTGTCCTGT GTTTCTTGGC 
TGTACCATTT GCGTAACTGG CTTAAGTAGT TCAGACAGGA AAGAAGTCCA GCGCCTCACG 
GCTGAACATG GTGGGCAGTA TTCAGGGCAG CTCAAGATGA ATGAATGTAC TCACCTCATA 
GTTCAAGAGC CAAAAGGTCA GAAATACGAA TGTGCCAAAA AATGGAATGT CCACTGCGTG 
CCTGTGCAGT GGTTTTCTGA CAGCATTGAG AAGGGCTTCT GTCAAGATGA GACAATGTAT 
AAAATAGAGA GTGGATCAAA ACTAAGTAGC ACACCCAGTA CCTCAACACC CACCAGCCAC 
GCCAGCAAGC TTGACACTCA TTCCCTTTCG GATGTCAGCT GCATTTCCAA TGTAAATTTG 
AGTAGAGTTA ATGAAACTGC TTGTAGTTCT GCAATGAGCA GCAGACTGGA TCCTCCTCCT 
GATGAGTTGG AAAACTTGGA CATAAGCTCT TTTCAAGCTC CTGAAGATCT ATTAGATGGC 
TGTCGGGTCT ATCTGTGTGG CTTCAGTGGC AGGAAGCTGG ACAAGATGAG AAGGCTTATT 
AACTGTGGTG GTGGTGTTCG ATTTAATCAG CTTAATGAAG ATGTTACCCA CGTCATTCTG 
GGGGAAAACA GTGACGAACT GAAACACTTT TTGGACAAGA CGCTTCACAG GCCTCATATA 
GTGACAGCAA AATGGCTACT GGAGTCATTT AATAAAGGCT ATCTCTGTCC AGTGGAGCAG 
TATATCCCTC AAAACTACCA GCTGTTAGCG AATACAATTT TGGAACAACC CGATGCAAAG 
TTAGTTCTTC CCAAAAACAA CAGCATGTCG AAGAAGAAAG CTGTAAATGT TGTGAAGCTC 
CAGAAAGCTG CAGAAGACGA CTTCCTCTCT CAGTATGTCA GTAATGATTC TACATTAGTT 
GAAGCTGAGA AACTAGCATC TGGTAGCTTC AATGATGTTA CTCATGTGAC TGTTCAAGAA 
GAGAATCATT CTTCTGTCTG TAATGGTTCT TTGGGAGAGC CTTCTGCACT GGATGAAGGT 
TTATTTGCCA GGAAGAGATT TCTTCTTTTG GGTTTTGGAG AAGAAGATGA GTCCTGCATT 
GCAGATCTGA TTAAGGAGCA TGCTGGGAAA ACTGTGCCGC TGCAAAGCAG AATCATTGCA 
GACTATGCTG TGGTACCTTT ATTGGGGTGC TCTGTGGAGT CAACTGTTGG TGATGTTGTC 
ACGAATACAT GGCTGGTAAC GTGTGTGGAA CAGCAGCTCC TCTTAGATCC CCAGTCCAAT 
CCACTTTTCA CACCAGTCCC AGTAATGGAA GGAGTTACTC CTTTGGTGGA CTGTGTTCTT 
TCTTTTAGCC AATTTACTGG TGCAGAGAGA GACTCCCTGG TTTATCTGGC AGGACTTCTA 
GGAGCAAGAG TCCAGGAATT CTTCGTGAGA AAAGCCAATG CAAAAAAAGG AATGTTTGCA 
AGTACCCATC TTGTGGTGAA AGAGCCGGAT GGTTCCAAGT ATGAGGCTGC AAAGAAGTGG 
AATTTGCCTG CGGTCACTGT AGCTTGGCTT TTGCAGTCTG CAAGGACAGG AAAGAGAGCA 
GATGAAAGCA AGTTTTTGGT TGAACATGCA GATGCTGAAG ATGAGAGTTC CATTACTAAA 
CTTAGCAAGA CACCACCAAC TACTAAATTT CCTGATTCTC CAGAGCGACC TACTTATCTC 
CGCGAAGCTG GAAAAAAACC AGCCGTCACC CCTCTTGATA TCAACAGGTT TCAGAGTAAA 
GCTTTCCAAG CTGTAATCTC TCATCATATT GGGAAGGCAA CAACTTCTCC TCCACCAAGA 
GGGCTGCTAC AAAAAGAACC ATCTTTACAC CTTGATACAC CATCAAAATT TCTTTCCAAA 
GACAAGTTGT TCAAGCCTTC TTTTGATGTA AAGGATGCTC TGGCAGCTTT GGAAACTCCA 
GGAGGTCTTG GTCAAAAGAA CAGGAAGCTG AGCACACCTC TTTCTGAAGT CATTGGCAGA 
AACCTGAAAT TGGCACTGGC AAACAGCACT CGGAATACAG AAGCTCTTAC TGCCAGCCCT 
CAGCTGAAGG CTGCACAGCC AGAAGTGGAA GAAGAGCCCA AGCCTCTAGC TGGTGTTGTT 
ATATGTGTTA GTAAAAAACT TACTAAGAAG CAAAGTGAAC TGAACGCTAT AGCAGCTTCT 
CTTGGTGCAG ACTACAGATG GTGTTTTGAT GAAACAGTAA CACACTTCAT CTACAAGGGA 
GGACAAAACG ACAGCAACAA GGAATATAGA TCTGTTAAAG AACGAGGAAT ACATATTGTT 
TCAGAACACT GGCTTTTAGA GTGTGCCCAA GAATATAAAC GGCTTCCTGA ATCTCTCTTT 
CCTCACACTT ACAATCCCAA AATGAGCCTG GACATCAGCG CGGTGCAAGA TTTCAGGCCC 
TCCTCTTCCA GTGAGCTTCC ACCCACTGGA AAACCAGCAG AAGAAAATGA GATTATTGCA 
GTTGATGAAG ACGATGCTGA AGATGATTTA ACTACTGATC AAATAAAGGA AACAGCTGGC 
ACAGGGGAAG AGCACAATGG AATAAGTGAA TCCAAGGGAG TTTTAACCCA GGCGCTAGAA 
ATGAGGGAGA ACTTCCAGAG GCAGCTGCAG GAAATCATGT CTGCGACATC AATAGTGAAA 
CCACAAGGAC AAAGAGGATC CCTGTCAAGG AACAGTTTTG ATAGTTCTCC AGCCACTCCT 
GATAGCGCCC GGTCTGTGCG AAATGGCCGC AGCAGAGCCT TGGAAGCTCT AAGGCAATCT 
CGTCAGGCTT TCACAGATAT CAACACGGAG CCATCCCAGA GTGAGCAGAT CATCTGGGAT 
GATCCTACAG CAAGAGAGGA AAGAGCAAGA CTTGTCAGCA ACTTCCAGTG GCCTAACAGT 
CCTTCCCAGT ACACAGAGCA AGCTCAGAGT AACGTCAATA GGAACACGGA TGAGTCTACC 
TTCAAAGGAT CCATAGCTGA TGCAGAGATT GCTGGTATAG TTGTTCCTGA GGCTGGGGAT 
GGAGATACGA TTGAAGGTCT GAAGAATCCT GTATGTAGAG ATCCTGAAAC ACCCATTAAA 
GATCACTTGA TCCCCACTCC ACAGGCTCCC AGCATTGCAT TCCCACTGGC TAACCCTCCT 
GTGGCACCAC AGCCCAAAGA AAAGGCTGTT ACAGAAGATG AGAAGGTAGA TGAAGAACCA 
GAAAAATGCC GTAAATTTCA GCTGTCTTCT CTTAATCCTC AAGAAAGATT TGACTACTGC 
CATCTGATTG AGGAATTAGG TGGAATAGTA CTTGAGAAAC AGTGCTTTGA TCCAAGTTGC 
ACACACATTG TTGTGGGACA TCCTCTTCGA AATGAGAAAT TCCTGGCCTC GATGGCAGCT 
GGAAAGTGGG TGCTTCATCG TTCCTACCTG GAGGCATGCA GAGGAGCTGG CTGCTTTGTT 
CAGGAAGAAG ATTATGAGTG GGGAAGTGAT TCCATACTTA ATGTTTTGCC TGGAATCAAT 
GTAAACCAGA AGAAGCTAGC GCTCGCAGCC ATGAGGTGGA GGAAAAGAAT TCATAAAGGA 
AGGCAAGAAA CTGGAATTAC TGAGGGAGCC TTCAGTGGCT GGAAGGTGAT CCTGAATGTT 
GATCACACCA AGGAAGCAGG ATTCCGACGC CTTCTTCAGT CAGGAGGAGC AAAAGTGTTT 
TCTGGTCATT CTGTGTCTTT CTTCAAAGAA GCGACTCATC TCTTCGCTGA CTTCAGTAAG 
CTGAAGCCAG ATGACACCAG GGTGAACATA GCAGAGGCGG CAGAGCAGGG AGTGAACTGC 
TTGAAGCCAG AGTACATTGC AGACTTCCTC ATCCAGGATC CACCTCCCCC AATGGAGTCT 
TACTGCCTGC CAGAAGCTGA GCGTTGCTTC CAGAACAACA AAGAACGTGG GACTGGATTA 
TCCCAGAAAA GAAAAGCACC GGGAGAAATG AGCAGAGTCA AGCGATCCAG AATGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TOPBP1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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