thbs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	thbs1
Entrez Gene ID	100170153
Full Name	thrombospondin 1
Synonyms	thbs,thbs-1,tsp,tsp-1,tsp1
General protein information	Preferred Names thrombospondin 1 Names thrombospondin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937199.4	XP_002937245.1	thrombospondin-1
XM_002937199.3	XP_002937245.1	thrombospondin-1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	THBS1	XP_001093770.2
Mus musculus (house mouse)	Thbs1	NP_035710.2
Gallus gallus (chicken)	THBS1	NP_001186382.1
Pan troglodytes (chimpanzee)	THBS1	XP_510294.3
Canis lupus familiaris (dog)	THBS1	XP_544610.3
Bos taurus (cattle)	THBS1	NP_776621.1
Rattus norvegicus (Norway rat)	Thbs1	NP_001013080.1
Homo sapiens (human)	THBS1	NP_003237.2
Danio rerio (zebrafish)	thbs1	XP_005160819.1
Xenopus tropicalis (tropical clawed frog)	thbs1	XP_002937245.1

Gene Ontology

The following thbs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thbs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa15101	XM_002937199.3 Latest version!	Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa64263	XM_002937199.4 Latest version!	Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thbs1 ( XM_002937199.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937245.1 Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.

Price & Availability↑

CloneID	OXa15101
Clone ID Related Accession (Same CDS sequence)	XM_002937199.3
Accession Version	XM_002937199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATGA TGAAGGGCAT TTTCCTACTG TTGATGTTGG CCGTGCCTCA AACATATCAA 
GCAGCAGAAT CTGGGAATGA TGATAACAGT GTATTTGATC TGTTTGAGTT AACGGGATAC 
AACCGCAAAA CTGGTTCTCG TAAGCCTGAA GGACTCCATT TAGTAAAGGG ACCTGACCCT 
TCAAGCCCAG CTTATAGAAT TGAAGATGCT GACTTGATCC CACCACTGCC AGAAGTCAAA 
TTTCAAGATT TGCTGGATGC AATACGTGCT GATAGGGGGT TCATTTTGTT GGCGACTCTG 
CGTCAAGCAA AGAAAAGTCG AGGGGCACTT CTGTCTGTGG AGAGAAAAGA TGGCGCAGGG 
CACATCTTCA GTCTGATCTC CAATGGCCGG GCTGGAACCC TAGATTTAAG TTTAAGTGGA 
GAAAGGAAGC AGCAGGTGGT GTCTGTAGAA GATGCTCTCC TAGCTACTGG CCACTGGAAG 
AACATCACCC TCTTTGTACA AGATGACCGT GCACAGCTCT ATGTAGGATG TGACAAGATG 
GAAAATGCTG AGCTAGATGT GCCAATACAG AAAATATTTA CTGAGGACCT GGCAAGCAAG 
GCTCATCTTC GAGTAGCAAA GGGAGGAGTA AAGGACAACT TTCAGGGAGT CCTTCAGAAT 
GTACGCTTTG TGTTTGGAAC CACATTGGAA GCTATTCTGA GAAACAAAGG CTGCTCAAGC 
ATGACAAACT CAGTCATCAC TTTGGACAAC CCAGTGAACG GCTCAAGCCC AGCCATCCGA 
ACGAATTACA TTGGCCACAA AACAAAGGAC CTGCATGCTG TGTGTGGCAT TTCCTGCGAT 
GATCTGTCCA AACTGTTTGC AGAAATGAAA GGCTTACGGA CGCTGGTCAC ATCTCTCCAG 
GACCAAGTCA CCAAAGAGAC GGAGAGGAAT GAACTTATTG CTCAGCGAGT AACAATGACG 
CCAGGTGCTT GTCTCCACAA TGGAGTCTTG CATAAGAACA GAGATGAGTG GACCGTTGAC 
AGCTGCACTG AATGCACATG TCAGAACTCA GCTACCATAT GCCGTAAGGT ATCTTGCCCT 
CTGATGCCCT GTACCAATGC CACCATTCCA GATGGGGAAT GCTGTCCCAG ATGTTGGCCA 
AGCGACTCAG CTGATGATGA TTGGTCTCCA TGGTCAGATT GGACACCTTG CTCTGTGACA 
TGTGGACATG GCATCCAGCA GAGAGGACGT TCCTGCGACA GCCTTAATAA CCGCTGCGAA 
GGTTCTTCTG TACAGACACG ATCTTGCCAT ATCCAGGAAT GTGACAAGAG ATTTAAGCAA 
GATGGTGGAT GGAGCCACTG GTCACCTTGG TCATCATGCT CAGTTACCTG CGGAAGTGGT 
CAAATCACCA GAATCCGTCT CTGCAACTCC CCTGTTCCTC AGCTGAATGG AAAGCCATGC 
GAAGGGGAAG GAAGGGAGAA CAAGCCATGT CAGAAAGACC CATGCCCAAT TAATGGTCAG 
TGGGGTCCTT GGTCACTGTG GGACACATGC ACTGTTACTT GCGGTGGTGG AATGATGAAG 
AGGGAGCGTC TCTGCAATAA CCCCAAACCT CAGTATGGGG GTAAAGAGTG TATTGGAGAT 
CCTACTGATT CTCAGATCTG CAACAAACAG GACTGTCCCA TAGATGGATG TCTCTCTAAC 
CCTTGCTTTG CTGGAGTCAA ATGCACCAGC TTCATTGATG GATCTTGGAA ATGTGGTTCC 
TGCCCACCAG GGTACAGAGG AAATGGAATA ACATGCAAAG ATATTGATGA GTGCAAAGAG 
GTGCCTGATG CCTGCTTCAG CCTGAATGGA GTTCATAGAT GTGAGAATAC AGAACCAGGA 
TATAATTGTC TCCCATGCCC TCCTCGGTTC ACAGGAACTC AGCCATTTGG CAAAGGCATT 
GATGAAGCTA AAGCCAATAA GCAGGTTTGC AAGCCTCGCA ACCCATGCGC CGATGGTACC 
CACGACTGTC ACAGGAACGC CAGATGTATC TACCTTGGAC ACTACAGCGA CCCCATGTAC 
CGATGTGAGT GCAAACCTGG ATACGCAGGA AATGGAATCA TTTGTGGCGA AGACACAGAT 
TTGGATGGCT GGCCAAACGA GAACCTCACA TGTGTTGCTA ATGCTACCTA TCACTGCATG 
AAAGATAACT GCCCCAACCT CCCCAACTCA GGCCAGGAAG ACTATGATAA GGATGGAATG 
GGAGATGCCT GCGATAAAGA CGATGACAAT GATGGAATCC TAGATGACAG GGACAACTGT 
CAGTTTGTGT ATAACCCAGC ACAGTACGAC TATGATCGTG ATGACGTGGG AGACCGGTGT 
GACAACTGCC CTTACAACCA CAACCCAGAC CAGGCTGACA CAGACCGCAA TGGAGAAGGA 
GATGCCTGTG CTGTGGACAT TGATGGAGAT GGGATCCTAA ACGAGAGGGA TAACTGCCCC 
TATGTGTACA ATGTCGACCA GAGAGACACA GATAAGGATG GTGTTGGAGA CCAATGTGAT 
AACTGCCCAC TGGAACACAA TCCAGAACAG ACTGACTCTG ATTCTGACCT CATTGGCGAT 
AAGTGTGACA ACAACCAAGA TATAGATGAG GATGGGCATC AAAATAACCT AGATAACTGC 
CCCTACATCC CCAATGCCAA CCAAGCAGAT CATGATAAGG ATGGCAAGGG TGATGCATGT 
GACCATGATG ATGACAATGA TGGTGTTCCT GATGACAAAG ATAACTGCCG ATTGGTCCCT 
AACCCAGACC AAACAGATTC TAATGGTGAT GGCCGTGGAG ATGCTTGCCA ATATGACTTT 
GATGATGATA ACATACCCGA TGCAGAAGAT GTTTGCCCAG AGAATGTGGA AATCAGCACT 
ACTGACTTCC GTAAATTCCA GATGGTGCCT TTGGATCCCA AAGGGACATC ACAGATTGAT 
CCTAACTGGG TGGTGAGACA CCAAGGCAAA GAACTGGTTC AGACTGTTAA CTGTGATCCT 
GGAATTGCAG TTGGTTTTGA CGAGTTCAGT GCTGTTGACT TCAGTGGCAC ATTTTTCATC 
AACACCGAGA GAGATGACGA TTATGCCGGC TTTGTGTTTG GATACCAATC CAGCAGCAGG 
TTTTACGTAG TCATGTGGAA GCAAATCACT CAGACATACT GGGACACAAC TCCCACTGTA 
GCTCAGGGCT ACTCTGGCCT CTCAATCAAG GTTGTGAACT CTACCAGCGG ACCAGGGGAG 
CACCTACGAA ATGCACTGTG GCACACAGGA AACACCCCTG GGCAGGTTCG CACCTTGTGG 
CATGATCCTC ACCAGATCGG CTGGAAAGAT TTTACAGCTT ACAGATGGCA CCTGACCCAC 
AGACCAAAGA CCGGTTTCAT TAGAGTCGTC ATGTATGAAG GCAAGAAAGT TATGGCTGAC 
TCAGGACCAA TCTATGATAA AACATATGCT GGAGGAAGAT TAGGATTATT CGTCTTCTCA 
CAAGAGATGG TCTTCTTTTC TGATCTCAAA TACGAGTGCA GAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937245.1
CDS	157..3687
Translation	MGMMKGIFLLLMLAVPQTYQAAESGNDDNSVFDLFELTGYNRKTGSRKPEGLHLVKGPDPSSPAYRIEDADLIPPLPEVKFQDLLDAIRADRGFILLATLRQAKKSRGALLSVERKDGAGHIFSLISNGRAGTLDLSLSGERKQQVVSVEDALLATGHWKNITLFVQDDRAQLYVGCDKMENAELDVPIQKIFTEDLASKAHLRVAKGGVKDNFQGVLQNVRFVFGTTLEAILRNKGCSSMTNSVITLDNPVNGSSPAIRTNYIGHKTKDLHAVCGISCDDLSKLFAEMKGLRTLVTSLQDQVTKETERNELIAQRVTMTPGACLHNGVLHKNRDEWTVDSCTECTCQNSATICRKVSCPLMPCTNATIPDGECCPRCWPSDSADDDWSPWSDWTPCSVTCGHGIQQRGRSCDSLNNRCEGSSVQTRSCHIQECDKRFKQDGGWSHWSPWSSCSVTCGSGQITRIRLCNSPVPQLNGKPCEGEGRENKPCQKDPCPINGQWGPWSLWDTCTVTCGGGMMKRERLCNNPKPQYGGKECIGDPTDSQICNKQDCPIDGCLSNPCFAGVKCTSFIDGSWKCGSCPPGYRGNGITCKDIDECKEVPDACFSLNGVHRCENTEPGYNCLPCPPRFTGTQPFGKGIDEAKANKQVCKPRNPCADGTHDCHRNARCIYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLTCVANATYHCMKDNCPNLPNSGQEDYDKDGMGDACDKDDDNDGILDDRDNCQFVYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDRNGEGDACAVDIDGDGILNERDNCPYVYNVDQRDTDKDGVGDQCDNCPLEHNPEQTDSDSDLIGDKCDNNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDHDDDNDGVPDDKDNCRLVPNPDQTDSNGDGRGDACQYDFDDDNIPDAEDVCPENVEISTTDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFSAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWDTTPTVAQGYSGLSIKVVNSTSGPGEHLRNALWHTGNTPGQVRTLWHDPHQIGWKDFTAYRWHLTHRPKTGFIRVVMYEGKKVMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_002937199.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937199.3

ORF Insert Sequence:

ATGGGGATGA TGAAGGGCAT TTTCCTACTG TTGATGTTGG CCGTGCCTCA AACATATCAA 
GCAGCAGAAT CTGGGAATGA TGATAACAGT GTATTTGATC TGTTTGAGTT AACGGGATAC 
AACCGCAAAA CTGGTTCTCG TAAGCCTGAA GGACTCCATT TAGTAAAGGG ACCTGACCCT 
TCAAGCCCAG CTTATAGAAT TGAAGATGCT GACTTGATCC CACCACTGCC AGAAGTCAAA 
TTTCAAGATT TGCTGGATGC AATACGTGCT GATAGGGGGT TCATTTTGTT GGCGACTCTG 
CGTCAAGCAA AGAAAAGTCG AGGGGCACTT CTGTCTGTGG AGAGAAAAGA TGGCGCAGGG 
CACATCTTCA GTCTGATCTC CAATGGCCGG GCTGGAACCC TAGATTTAAG TTTAAGTGGA 
GAAAGGAAGC AGCAGGTGGT GTCTGTAGAA GATGCTCTCC TAGCTACTGG CCACTGGAAG 
AACATCACCC TCTTTGTACA AGATGACCGT GCACAGCTCT ATGTAGGATG TGACAAGATG 
GAAAATGCTG AGCTAGATGT GCCAATACAG AAAATATTTA CTGAGGACCT GGCAAGCAAG 
GCTCATCTTC GAGTAGCAAA GGGAGGAGTA AAGGACAACT TTCAGGGAGT CCTTCAGAAT 
GTACGCTTTG TGTTTGGAAC CACATTGGAA GCTATTCTGA GAAACAAAGG CTGCTCAAGC 
ATGACAAACT CAGTCATCAC TTTGGACAAC CCAGTGAACG GCTCAAGCCC AGCCATCCGA 
ACGAATTACA TTGGCCACAA AACAAAGGAC CTGCATGCTG TGTGTGGCAT TTCCTGCGAT 
GATCTGTCCA AACTGTTTGC AGAAATGAAA GGCTTACGGA CGCTGGTCAC ATCTCTCCAG 
GACCAAGTCA CCAAAGAGAC GGAGAGGAAT GAACTTATTG CTCAGCGAGT AACAATGACG 
CCAGGTGCTT GTCTCCACAA TGGAGTCTTG CATAAGAACA GAGATGAGTG GACCGTTGAC 
AGCTGCACTG AATGCACATG TCAGAACTCA GCTACCATAT GCCGTAAGGT ATCTTGCCCT 
CTGATGCCCT GTACCAATGC CACCATTCCA GATGGGGAAT GCTGTCCCAG ATGTTGGCCA 
AGCGACTCAG CTGATGATGA TTGGTCTCCA TGGTCAGATT GGACACCTTG CTCTGTGACA 
TGTGGACATG GCATCCAGCA GAGAGGACGT TCCTGCGACA GCCTTAATAA CCGCTGCGAA 
GGTTCTTCTG TACAGACACG ATCTTGCCAT ATCCAGGAAT GTGACAAGAG ATTTAAGCAA 
GATGGTGGAT GGAGCCACTG GTCACCTTGG TCATCATGCT CAGTTACCTG CGGAAGTGGT 
CAAATCACCA GAATCCGTCT CTGCAACTCC CCTGTTCCTC AGCTGAATGG AAAGCCATGC 
GAAGGGGAAG GAAGGGAGAA CAAGCCATGT CAGAAAGACC CATGCCCAAT TAATGGTCAG 
TGGGGTCCTT GGTCACTGTG GGACACATGC ACTGTTACTT GCGGTGGTGG AATGATGAAG 
AGGGAGCGTC TCTGCAATAA CCCCAAACCT CAGTATGGGG GTAAAGAGTG TATTGGAGAT 
CCTACTGATT CTCAGATCTG CAACAAACAG GACTGTCCCA TAGATGGATG TCTCTCTAAC 
CCTTGCTTTG CTGGAGTCAA ATGCACCAGC TTCATTGATG GATCTTGGAA ATGTGGTTCC 
TGCCCACCAG GGTACAGAGG AAATGGAATA ACATGCAAAG ATATTGATGA GTGCAAAGAG 
GTGCCTGATG CCTGCTTCAG CCTGAATGGA GTTCATAGAT GTGAGAATAC AGAACCAGGA 
TATAATTGTC TCCCATGCCC TCCTCGGTTC ACAGGAACTC AGCCATTTGG CAAAGGCATT 
GATGAAGCTA AAGCCAATAA GCAGGTTTGC AAGCCTCGCA ACCCATGCGC CGATGGTACC 
CACGACTGTC ACAGGAACGC CAGATGTATC TACCTTGGAC ACTACAGCGA CCCCATGTAC 
CGATGTGAGT GCAAACCTGG ATACGCAGGA AATGGAATCA TTTGTGGCGA AGACACAGAT 
TTGGATGGCT GGCCAAACGA GAACCTCACA TGTGTTGCTA ATGCTACCTA TCACTGCATG 
AAAGATAACT GCCCCAACCT CCCCAACTCA GGCCAGGAAG ACTATGATAA GGATGGAATG 
GGAGATGCCT GCGATAAAGA CGATGACAAT GATGGAATCC TAGATGACAG GGACAACTGT 
CAGTTTGTGT ATAACCCAGC ACAGTACGAC TATGATCGTG ATGACGTGGG AGACCGGTGT 
GACAACTGCC CTTACAACCA CAACCCAGAC CAGGCTGACA CAGACCGCAA TGGAGAAGGA 
GATGCCTGTG CTGTGGACAT TGATGGAGAT GGGATCCTAA ACGAGAGGGA TAACTGCCCC 
TATGTGTACA ATGTCGACCA GAGAGACACA GATAAGGATG GTGTTGGAGA CCAATGTGAT 
AACTGCCCAC TGGAACACAA TCCAGAACAG ACTGACTCTG ATTCTGACCT CATTGGCGAT 
AAGTGTGACA ACAACCAAGA TATAGATGAG GATGGGCATC AAAATAACCT AGATAACTGC 
CCCTACATCC CCAATGCCAA CCAAGCAGAT CATGATAAGG ATGGCAAGGG TGATGCATGT 
GACCATGATG ATGACAATGA TGGTGTTCCT GATGACAAAG ATAACTGCCG ATTGGTCCCT 
AACCCAGACC AAACAGATTC TAATGGTGAT GGCCGTGGAG ATGCTTGCCA ATATGACTTT 
GATGATGATA ACATACCCGA TGCAGAAGAT GTTTGCCCAG AGAATGTGGA AATCAGCACT 
ACTGACTTCC GTAAATTCCA GATGGTGCCT TTGGATCCCA AAGGGACATC ACAGATTGAT 
CCTAACTGGG TGGTGAGACA CCAAGGCAAA GAACTGGTTC AGACTGTTAA CTGTGATCCT 
GGAATTGCAG TTGGTTTTGA CGAGTTCAGT GCTGTTGACT TCAGTGGCAC ATTTTTCATC 
AACACCGAGA GAGATGACGA TTATGCCGGC TTTGTGTTTG GATACCAATC CAGCAGCAGG 
TTTTACGTAG TCATGTGGAA GCAAATCACT CAGACATACT GGGACACAAC TCCCACTGTA 
GCTCAGGGCT ACTCTGGCCT CTCAATCAAG GTTGTGAACT CTACCAGCGG ACCAGGGGAG 
CACCTACGAA ATGCACTGTG GCACACAGGA AACACCCCTG GGCAGGTTCG CACCTTGTGG 
CATGATCCTC ACCAGATCGG CTGGAAAGAT TTTACAGCTT ACAGATGGCA CCTGACCCAC 
AGACCAAAGA CCGGTTTCAT TAGAGTCGTC ATGTATGAAG GCAAGAAAGT TATGGCTGAC 
TCAGGACCAA TCTATGATAA AACATATGCT GGAGGAAGAT TAGGATTATT CGTCTTCTCA 
CAAGAGATGG TCTTCTTTTC TGATCTCAAA TACGAGTGCA GAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thbs1 ( XM_002937199.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937245.1 Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.

Price & Availability↑

CloneID	OXa64263
Clone ID Related Accession (Same CDS sequence)	XM_002937199.4
Accession Version	XM_002937199.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thrombospondin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937199.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGATGA TGAAGGGCAT TTTCCTACTG TTGATGTTGG CCGTGCCTCA AACATATCAA 
GCAGCAGAAT CTGGGAATGA TGATAACAGT GTATTTGATC TGTTTGAGTT AACGGGATAC 
AACCGCAAAA CTGGTTCTCG TAAGCCTGAA GGACTCCATT TAGTAAAGGG ACCTGACCCT 
TCAAGCCCAG CTTATAGAAT TGAAGATGCT GACTTGATCC CACCACTGCC AGAAGTCAAA 
TTTCAAGATT TGCTGGATGC AATACGTGCT GATAGGGGGT TCATTTTGTT GGCGACTCTG 
CGTCAAGCAA AGAAAAGTCG AGGGGCACTT CTGTCTGTGG AGAGAAAAGA TGGCGCAGGG 
CACATCTTCA GTCTGATCTC CAATGGCCGG GCTGGAACCC TAGATTTAAG TTTAAGTGGA 
GAAAGGAAGC AGCAGGTGGT GTCTGTAGAA GATGCTCTCC TAGCTACTGG CCACTGGAAG 
AACATCACCC TCTTTGTACA AGATGACCGC GCACAGCTCT ATGTAGGATG TGACAAGATG 
GAAAATGCTG AGCTAGATGT GCCAATACAG AAAATATTTA CTGAGGACCT GGCAAGCAAA 
GCTCATCTTC GAGTAGCAAA GGGAGGAGTA AAGGACAACT TTCAGGGAGT CCTTCAGAAT 
GTACGCTTTG TGTTTGGAAC CACATTGGAA GCTATTCTGA GAAACAAAGG CTGCTCAAGC 
ATGACAAACT CAGTCATCAC TTTGGACAAC CCAGTGAACG GCTCAAGCCC AGCCATCCGA 
ACGAATTACA TTGGCCACAA AACAAAGGAC CTGCATGCTG TGTGTGGCAT TTCCTGCGAT 
GATCTGTCCA AACTGTTTGC AGAAATGAAA GGCTTACGGA CGCTGGTCAC ATCTCTCCAG 
GACCAAGTCA CCAAAGAGAC GGAGAGGAAT GAACTTATTG CTCAGCGAGT AACAATGACG 
CCAGGTGCTT GTCTCCACAA TGGAGTCTTG CATAAGAACA GAGATGAGTG GACCGTTGAC 
AGCTGCACTG AATGCACATG TCAGAACTCA GCTACCATAT GCCGTAAGGT ATCTTGCCCT 
CTGATGCCCT GTACCAATGC CACCATTCCA GATGGGGAAT GCTGTCCCAG ATGTTGGCCA 
AGCGACTCAG CTGATGATGA TTGGTCTCCA TGGTCAGATT GGACACCTTG CTCTGTGACA 
TGTGGTCATG GCATCCAGCA GAGAGGACGT TCTTGCGACA GCCTTAATAA CCGCTGCGAA 
GGTTCTTCTG TACAGACACG ATCTTGCCAT ATCCAGGAAT GTGACAAGAG ATTTAAGCAA 
GATGGTGGAT GGAGCCACTG GTCACCTTGG TCATCATGCT CAGTTACCTG CGGAAGTGGT 
CAAATCACCA GAATCCGTCT CTGCAACTCC CCTGTTCCTC AGCTGAATGG AAAGCCATGC 
GAAGGGGAAG GAAGGGAGAA CAAGCCATGT CAGAAAGACC CATGCCCAAT TAATGGTCAG 
TGGGGTCCTT GGTCACTGTG GGACACATGC ACTGTTACTT GCGGTGGTGG AATGATGAAG 
AGGGAGCGTC TCTGCAATAA CCCCAAACCT CAGTATGGGG GTAAAGAGTG TATTGGAGAT 
CCTACTGATT CTCAGATCTG CAACAAACAG GACTGTCCCA TAGATGGATG TCTCTCTAAC 
CCTTGCTTTG CTGGAGTCAA ATGCACCAGC TTCATTGATG GATCTTGGAA ATGTGGTTCC 
TGCCCACCAG GGTACAGAGG AAACGGAATA ACATGCAAAG ATATTGATGA GTGCAAAGAG 
GTGCCTGATG CCTGCTTCAG CCTGAATGGA GTTCATAGAT GTGAGAATAC AGAACCAGGA 
TATAATTGTC TCCCATGCCC TCCTCGGTTC ACAGGAACTC AGCCATTTGG CAAAGGCATT 
GATGAAGCTA AAGCCAATAA GCAGGTTTGC AAGCCTCGCA ACCCATGCGC CGATGGTACC 
CACGACTGTC ACAGGAACGC CAGATGTATC TACCTTGGAC ACTACAGCGA CCCCATGTAC 
CGCTGTGAGT GCAAACCTGG ATACGCAGGA AATGGAATCA TTTGTGGTGA AGACACAGAT 
TTGGATGGCT GGCCAAACGA GAACCTCACA TGTGTTGCTA ATGCTACCTA TCACTGCATG 
AAAGATAACT GCCCCAACCT CCCCAACTCA GGCCAGGAAG ACTATGATAA GGATGGAATG 
GGAGATGCCT GCGATAAAGA CGATGACAAT GATGGAATCC TAGATGACAG GGACAACTGT 
CAGTTTGTGT ATAACCCAGC ACAGTATGAC TATGATCGTG ATGACGTGGG AGACCGGTGT 
GACAACTGCC CTTACAACCA CAACCCAGAC CAGGCTGACA CAGACCGCAA TGGAGAAGGA 
GATGCCTGTG CTGTGGACAT TGATGGAGAT GGGATCCTAA ACGAGAGGGA TAACTGCCCC 
TATGTGTACA ATGTCGACCA GAGAGACACA GATAAGGATG GTGTTGGAGA CCAATGTGAT 
AACTGCCCAC TGGAACACAA TCCAGAACAG ACTGACTCTG ATTCTGACCT CATTGGCGAT 
AAGTGTGACA ACAACCAAGA TATAGATGAG GATGGGCATC AAAATAACCT AGATAACTGC 
CCCTACATCC CCAATGCCAA CCAAGCAGAT CATGATAAGG ATGGCAAGGG TGATGCATGT 
GACCATGATG ATGACAATGA TGGTGTTCCT GATGACAAAG ATAACTGCCG ATTGGTCCCT 
AACCCAGACC AAACAGATTC TAATGGTGAT GGCCGTGGAG ATGCTTGCCA ATATGACTTT 
GATGATGATA ACATACCCGA TGCAGAAGAT GTTTGCCCAG AGAATGTGGA AATCAGCACT 
ACTGACTTCC GTAAATTCCA GATGGTGCCT TTGGATCCCA AAGGGACATC ACAGATTGAT 
CCTAACTGGG TGGTGAGACA CCAAGGCAAA GAATTGGTTC AGACTGTTAA CTGTGATCCT 
GGAATTGCAG TTGGTTTTGA CGAGTTCAGT GCTGTTGACT TCAGTGGCAC ATTTTTCATC 
AACACCGAGA GAGATGACGA TTATGCCGGC TTTGTGTTTG GATACCAATC CAGCAGCAGG 
TTTTACGTAG TCATGTGGAA GCAAATCACT CAGACATACT GGGACACAAC TCCCACTGTA 
GCTCAGGGCT ACTCTGGCCT CTCAATCAAG GTTGTGAACT CTACCAGCGG ACCAGGGGAG 
CACCTACGAA ATGCACTGTG GCACACAGGA AACACCCCTG GGCAGGTTCG CACCTTGTGG 
CATGATCCTC ACCAGATCGG CTGGAAAGAT TTTACAGCTT ACAGATGGCA CCTGACCCAC 
AGACCAAAGA CCGGTTTCAT TAGAGTCGTC ATGTATGAAG GCAAGAAAGT TATGGCTGAC 
TCAGGACCAA TCTATGATAA AACATATGCT GGAGGAAGAT TAGGATTATT CGTCTTCTCA 
CAAGAGATGG TCTTCTTTTC TGATCTCAAA TACGAGTGCA GAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937245.1
CDS	181..3711
Translation	MGMMKGIFLLLMLAVPQTYQAAESGNDDNSVFDLFELTGYNRKTGSRKPEGLHLVKGPDPSSPAYRIEDADLIPPLPEVKFQDLLDAIRADRGFILLATLRQAKKSRGALLSVERKDGAGHIFSLISNGRAGTLDLSLSGERKQQVVSVEDALLATGHWKNITLFVQDDRAQLYVGCDKMENAELDVPIQKIFTEDLASKAHLRVAKGGVKDNFQGVLQNVRFVFGTTLEAILRNKGCSSMTNSVITLDNPVNGSSPAIRTNYIGHKTKDLHAVCGISCDDLSKLFAEMKGLRTLVTSLQDQVTKETERNELIAQRVTMTPGACLHNGVLHKNRDEWTVDSCTECTCQNSATICRKVSCPLMPCTNATIPDGECCPRCWPSDSADDDWSPWSDWTPCSVTCGHGIQQRGRSCDSLNNRCEGSSVQTRSCHIQECDKRFKQDGGWSHWSPWSSCSVTCGSGQITRIRLCNSPVPQLNGKPCEGEGRENKPCQKDPCPINGQWGPWSLWDTCTVTCGGGMMKRERLCNNPKPQYGGKECIGDPTDSQICNKQDCPIDGCLSNPCFAGVKCTSFIDGSWKCGSCPPGYRGNGITCKDIDECKEVPDACFSLNGVHRCENTEPGYNCLPCPPRFTGTQPFGKGIDEAKANKQVCKPRNPCADGTHDCHRNARCIYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLTCVANATYHCMKDNCPNLPNSGQEDYDKDGMGDACDKDDDNDGILDDRDNCQFVYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDRNGEGDACAVDIDGDGILNERDNCPYVYNVDQRDTDKDGVGDQCDNCPLEHNPEQTDSDSDLIGDKCDNNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDHDDDNDGVPDDKDNCRLVPNPDQTDSNGDGRGDACQYDFDDDNIPDAEDVCPENVEISTTDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFSAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWDTTPTVAQGYSGLSIKVVNSTSGPGEHLRNALWHTGNTPGQVRTLWHDPHQIGWKDFTAYRWHLTHRPKTGFIRVVMYEGKKVMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	XM_002937199.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thrombospondin 1 (thbs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937199.4

ORF Insert Sequence:

ATGGGGATGA TGAAGGGCAT TTTCCTACTG TTGATGTTGG CCGTGCCTCA AACATATCAA 
GCAGCAGAAT CTGGGAATGA TGATAACAGT GTATTTGATC TGTTTGAGTT AACGGGATAC 
AACCGCAAAA CTGGTTCTCG TAAGCCTGAA GGACTCCATT TAGTAAAGGG ACCTGACCCT 
TCAAGCCCAG CTTATAGAAT TGAAGATGCT GACTTGATCC CACCACTGCC AGAAGTCAAA 
TTTCAAGATT TGCTGGATGC AATACGTGCT GATAGGGGGT TCATTTTGTT GGCGACTCTG 
CGTCAAGCAA AGAAAAGTCG AGGGGCACTT CTGTCTGTGG AGAGAAAAGA TGGCGCAGGG 
CACATCTTCA GTCTGATCTC CAATGGCCGG GCTGGAACCC TAGATTTAAG TTTAAGTGGA 
GAAAGGAAGC AGCAGGTGGT GTCTGTAGAA GATGCTCTCC TAGCTACTGG CCACTGGAAG 
AACATCACCC TCTTTGTACA AGATGACCGC GCACAGCTCT ATGTAGGATG TGACAAGATG 
GAAAATGCTG AGCTAGATGT GCCAATACAG AAAATATTTA CTGAGGACCT GGCAAGCAAA 
GCTCATCTTC GAGTAGCAAA GGGAGGAGTA AAGGACAACT TTCAGGGAGT CCTTCAGAAT 
GTACGCTTTG TGTTTGGAAC CACATTGGAA GCTATTCTGA GAAACAAAGG CTGCTCAAGC 
ATGACAAACT CAGTCATCAC TTTGGACAAC CCAGTGAACG GCTCAAGCCC AGCCATCCGA 
ACGAATTACA TTGGCCACAA AACAAAGGAC CTGCATGCTG TGTGTGGCAT TTCCTGCGAT 
GATCTGTCCA AACTGTTTGC AGAAATGAAA GGCTTACGGA CGCTGGTCAC ATCTCTCCAG 
GACCAAGTCA CCAAAGAGAC GGAGAGGAAT GAACTTATTG CTCAGCGAGT AACAATGACG 
CCAGGTGCTT GTCTCCACAA TGGAGTCTTG CATAAGAACA GAGATGAGTG GACCGTTGAC 
AGCTGCACTG AATGCACATG TCAGAACTCA GCTACCATAT GCCGTAAGGT ATCTTGCCCT 
CTGATGCCCT GTACCAATGC CACCATTCCA GATGGGGAAT GCTGTCCCAG ATGTTGGCCA 
AGCGACTCAG CTGATGATGA TTGGTCTCCA TGGTCAGATT GGACACCTTG CTCTGTGACA 
TGTGGTCATG GCATCCAGCA GAGAGGACGT TCTTGCGACA GCCTTAATAA CCGCTGCGAA 
GGTTCTTCTG TACAGACACG ATCTTGCCAT ATCCAGGAAT GTGACAAGAG ATTTAAGCAA 
GATGGTGGAT GGAGCCACTG GTCACCTTGG TCATCATGCT CAGTTACCTG CGGAAGTGGT 
CAAATCACCA GAATCCGTCT CTGCAACTCC CCTGTTCCTC AGCTGAATGG AAAGCCATGC 
GAAGGGGAAG GAAGGGAGAA CAAGCCATGT CAGAAAGACC CATGCCCAAT TAATGGTCAG 
TGGGGTCCTT GGTCACTGTG GGACACATGC ACTGTTACTT GCGGTGGTGG AATGATGAAG 
AGGGAGCGTC TCTGCAATAA CCCCAAACCT CAGTATGGGG GTAAAGAGTG TATTGGAGAT 
CCTACTGATT CTCAGATCTG CAACAAACAG GACTGTCCCA TAGATGGATG TCTCTCTAAC 
CCTTGCTTTG CTGGAGTCAA ATGCACCAGC TTCATTGATG GATCTTGGAA ATGTGGTTCC 
TGCCCACCAG GGTACAGAGG AAACGGAATA ACATGCAAAG ATATTGATGA GTGCAAAGAG 
GTGCCTGATG CCTGCTTCAG CCTGAATGGA GTTCATAGAT GTGAGAATAC AGAACCAGGA 
TATAATTGTC TCCCATGCCC TCCTCGGTTC ACAGGAACTC AGCCATTTGG CAAAGGCATT 
GATGAAGCTA AAGCCAATAA GCAGGTTTGC AAGCCTCGCA ACCCATGCGC CGATGGTACC 
CACGACTGTC ACAGGAACGC CAGATGTATC TACCTTGGAC ACTACAGCGA CCCCATGTAC 
CGCTGTGAGT GCAAACCTGG ATACGCAGGA AATGGAATCA TTTGTGGTGA AGACACAGAT 
TTGGATGGCT GGCCAAACGA GAACCTCACA TGTGTTGCTA ATGCTACCTA TCACTGCATG 
AAAGATAACT GCCCCAACCT CCCCAACTCA GGCCAGGAAG ACTATGATAA GGATGGAATG 
GGAGATGCCT GCGATAAAGA CGATGACAAT GATGGAATCC TAGATGACAG GGACAACTGT 
CAGTTTGTGT ATAACCCAGC ACAGTATGAC TATGATCGTG ATGACGTGGG AGACCGGTGT 
GACAACTGCC CTTACAACCA CAACCCAGAC CAGGCTGACA CAGACCGCAA TGGAGAAGGA 
GATGCCTGTG CTGTGGACAT TGATGGAGAT GGGATCCTAA ACGAGAGGGA TAACTGCCCC 
TATGTGTACA ATGTCGACCA GAGAGACACA GATAAGGATG GTGTTGGAGA CCAATGTGAT 
AACTGCCCAC TGGAACACAA TCCAGAACAG ACTGACTCTG ATTCTGACCT CATTGGCGAT 
AAGTGTGACA ACAACCAAGA TATAGATGAG GATGGGCATC AAAATAACCT AGATAACTGC 
CCCTACATCC CCAATGCCAA CCAAGCAGAT CATGATAAGG ATGGCAAGGG TGATGCATGT 
GACCATGATG ATGACAATGA TGGTGTTCCT GATGACAAAG ATAACTGCCG ATTGGTCCCT 
AACCCAGACC AAACAGATTC TAATGGTGAT GGCCGTGGAG ATGCTTGCCA ATATGACTTT 
GATGATGATA ACATACCCGA TGCAGAAGAT GTTTGCCCAG AGAATGTGGA AATCAGCACT 
ACTGACTTCC GTAAATTCCA GATGGTGCCT TTGGATCCCA AAGGGACATC ACAGATTGAT 
CCTAACTGGG TGGTGAGACA CCAAGGCAAA GAATTGGTTC AGACTGTTAA CTGTGATCCT 
GGAATTGCAG TTGGTTTTGA CGAGTTCAGT GCTGTTGACT TCAGTGGCAC ATTTTTCATC 
AACACCGAGA GAGATGACGA TTATGCCGGC TTTGTGTTTG GATACCAATC CAGCAGCAGG 
TTTTACGTAG TCATGTGGAA GCAAATCACT CAGACATACT GGGACACAAC TCCCACTGTA 
GCTCAGGGCT ACTCTGGCCT CTCAATCAAG GTTGTGAACT CTACCAGCGG ACCAGGGGAG 
CACCTACGAA ATGCACTGTG GCACACAGGA AACACCCCTG GGCAGGTTCG CACCTTGTGG 
CATGATCCTC ACCAGATCGG CTGGAAAGAT TTTACAGCTT ACAGATGGCA CCTGACCCAC 
AGACCAAAGA CCGGTTTCAT TAGAGTCGTC ATGTATGAAG GCAAGAAAGT TATGGCTGAC 
TCAGGACCAA TCTATGATAA AACATATGCT GGAGGAAGAT TAGGATTATT CGTCTTCTCA 
CAAGAGATGG TCTTCTTTTC TGATCTCAAA TACGAGTGCA GAGACTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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thbs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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