THBS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS1
Entrez Gene ID	7057
Full Name	thrombospondin 1
Synonyms	THBS,THBS-1,TSP,TSP-1,TSP1
General protein information	Preferred Names thrombospondin 1 Names thrombospondin-1 thrombospondin-1p180 thrombospondin-p50
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q14
Summary	The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	188060

mRNA and Protein(s)

mRNA	Protein	Name
NM_003246.4	NP_003237.2	thrombospondin-1 precursor
NM_003246.3	NP_003237.2	thrombospondin-1 precursor
XM_011521971.1	XP_011520273.1	thrombospondin-1 isoform X1
XM_011521970.1	XP_011520272.1	thrombospondin-1 isoform X1
XM_011521971.2	XP_011520273.1	thrombospondin-1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	THBS1	XP_001093770.2
Mus musculus (house mouse)	Thbs1	NP_035710.2
Gallus gallus (chicken)	THBS1	NP_001186382.1
Pan troglodytes (chimpanzee)	THBS1	XP_510294.3
Canis lupus familiaris (dog)	THBS1	XP_544610.3
Bos taurus (cattle)	THBS1	NP_776621.1
Rattus norvegicus (Norway rat)	Thbs1	NP_001013080.1
Homo sapiens (human)	THBS1	NP_003237.2
Danio rerio (zebrafish)	thbs1	XP_005160819.1
Xenopus tropicalis (tropical clawed frog)	thbs1	XP_002937245.1

Gene Ontology
Bibliography

Related articles in PubMed

[The poor prognosis is correlated with the high expression of vascular endothelial growth factor (VEGF) and low expression of thrombospondin 1 (TSP-1) in patients with breast cancer].
Huang Q, Huang Y, Li M, Zhang W
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology35(9)828-831(2019 Sep)

The Pathological Significance and Prognostic Roles of Thrombospondin-1, and -2, and 4N1K-peptide in Bladder Cancer.
Nakamura Y, Miyata Y, Takehara K, Asai A, Mitsunari K, Araki K, Matsuo T, Ohba K, Sakai H
Anticancer research39(5)2317-2324(2019 May)

Deciphering the complex role of thrombospondin-1 in glioblastoma development.
Daubon T, Léon C, Clarke K, Andrique L, Salabert L, Darbo E, Pineau R, Guérit S, Maitre M, Dedieu S, Jeanne A, Bailly S, Feige JJ, Miletic H, Rossi M, Bello L, Falciani F, Bjerkvig R, Bikfalvi A
Nature communications10(1)1146(2019 03)

The Increase of pFAK and THBS1 Protein and Gene Expression Levels in Vascular Smooth Muscle Cells by Histamine-treated M1 Macrophages.
Khosravi M, Najafi M, Amirfarhangi A, Karimi M, Fattahi F, Shabani M
Iranian journal of allergy, asthma, and immunology18(1)72-79(2019 Feb)

Thrombospondin 1 Is Increased in the Aorta and Plasma of Patients With Acute Aortic Dissection.
Zeng T, Yuan J, Gan J, Liu Y, Shi L, Lu Z, Xue Y, Xiong R, Huang M, Yang Z, Lin Y, Liu L
The Canadian journal of cardiology35(1)42-50(2019 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu60983	XM_011521971.2 Latest version!	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu27122	NM_003246.4 Latest version!	Homo sapiens thrombospondin 1 (THBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu27122	XM_011521970.1 Latest version!	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OHu27122	NM_003246.3	Homo sapiens thrombospondin 1 (THBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
OHu60983	XM_011521971.1	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS1 ( XM_011521971.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520273.1 Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60983
Clone ID Related Accession (Same CDS sequence)	XM_011521971.1 , XM_011521971.2
Accession Version	XM_011521971.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC AATGGATGCC TGTCCAATCC CTGCTTTGCC 
GGCGTGAAGT GTACTAGCTA CCCTGATGGC AGCTGGAAAT GTGGTGCTTG TCCCCCTGGT 
TACAGTGGAA ATGGCATCCA GTGCACAGAT GTTGATGAGT GCAAAGAAGT GCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACCGGTGT GAGAACACGG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCCC CACGCTTCAC CGGCTCACAG CCCTTCGGCC AGGGTGTCGA ACATGCCACG 
GCCAACAAAC AGGTGTGCAA GCCCCGTAAC CCCTGCACGG ATGGGACCCA CGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TATAGCGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCTGGCAA TGGCATCATC TGCGGGGAGG ACACAGACCT GGATGGCTGG 
CCCAATGAGA ACCTGGTGTG CGTGGCCAAT GCGACTTACC ACTGCAAAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATTGG TGATGCCTGT 
GATGATGACG ATGACAATGA TAAAATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCTC AGTATGACTA TGACAGAGAT GATGTGGGAG ACCGCTGTGA CAACTGTCCC 
TACAACCACA ACCCAGATCA GGCAGACACA GACAACAATG GGGAAGGAGA CGCCTGTGCT 
GCAGACATTG ATGGAGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA CGTCTACAAT 
GTGGACCAGA GAGACACTGA TATGGATGGG GTTGGAGATC AGTGTGACAA TTGCCCCTTG 
GAACACAATC CGGATCAGCT GGACTCTGAC TCAGACCGCA TTGGAGATAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA TGGCCACCAG AACAATCTGG ACAACTGTCC CTATGTGCCC 
AATGCCAACC AGGCTGACCA TGACAAAGAT GGCAAGGGAG ATGCCTGTGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACAAGGAC AACTGCAGAC TCGTGCCCAA TCCCGACCAG 
AAGGACTCTG ACGGCGATGG TCGAGGTGAT GCCTGCAAAG ATGATTTTGA CCATGACAGT 
GTGCCAGACA TCGATGACAT CTGTCCTGAG AATGTTGACA TCAGTGAGAC CGATTTCCGC 
CGATTCCAGA TGATTCCTCT GGACCCCAAA GGGACATCCC AAAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGATCCTGG ACTCGCTGTA 
GGTTATGATG AGTTTAATGC TGTGGACTTC AGTGGCACCT TCTTCATCAA CACCGAAAGG 
GACGATGACT ATGCTGGATT TGTCTTTGGC TACCAGTCCA GCAGCCGCTT TTATGTTGTG 
ATGTGGAAGC AAGTCACCCA GTCCTACTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCGGGCCTTT CTGTGAAAGT TGTAAACTCC ACCACAGGGC CTGGCGAGCA CCTGCGGAAC 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CCCTGTGGCA TGACCCTCGT 
CACATAGGCT GGAAAGATTT CACCGCCTAC AGATGGCGTC TCAGCCACAG GCCAAAGACG 
GGTTTCATTA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGATAAAA CCTATGCTGG TGGTAGACTA GGGTTGTTTG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCTG ACCTGAAATA CGAATGTAGA GATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520273.1
CDS	176..3514
Translation	MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPNGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	XM_011521971.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521971.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC AATGGATGCC TGTCCAATCC CTGCTTTGCC 
GGCGTGAAGT GTACTAGCTA CCCTGATGGC AGCTGGAAAT GTGGTGCTTG TCCCCCTGGT 
TACAGTGGAA ATGGCATCCA GTGCACAGAT GTTGATGAGT GCAAAGAAGT GCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACCGGTGT GAGAACACGG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCCC CACGCTTCAC CGGCTCACAG CCCTTCGGCC AGGGTGTCGA ACATGCCACG 
GCCAACAAAC AGGTGTGCAA GCCCCGTAAC CCCTGCACGG ATGGGACCCA CGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TATAGCGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCTGGCAA TGGCATCATC TGCGGGGAGG ACACAGACCT GGATGGCTGG 
CCCAATGAGA ACCTGGTGTG CGTGGCCAAT GCGACTTACC ACTGCAAAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATTGG TGATGCCTGT 
GATGATGACG ATGACAATGA TAAAATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCTC AGTATGACTA TGACAGAGAT GATGTGGGAG ACCGCTGTGA CAACTGTCCC 
TACAACCACA ACCCAGATCA GGCAGACACA GACAACAATG GGGAAGGAGA CGCCTGTGCT 
GCAGACATTG ATGGAGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA CGTCTACAAT 
GTGGACCAGA GAGACACTGA TATGGATGGG GTTGGAGATC AGTGTGACAA TTGCCCCTTG 
GAACACAATC CGGATCAGCT GGACTCTGAC TCAGACCGCA TTGGAGATAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA TGGCCACCAG AACAATCTGG ACAACTGTCC CTATGTGCCC 
AATGCCAACC AGGCTGACCA TGACAAAGAT GGCAAGGGAG ATGCCTGTGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACAAGGAC AACTGCAGAC TCGTGCCCAA TCCCGACCAG 
AAGGACTCTG ACGGCGATGG TCGAGGTGAT GCCTGCAAAG ATGATTTTGA CCATGACAGT 
GTGCCAGACA TCGATGACAT CTGTCCTGAG AATGTTGACA TCAGTGAGAC CGATTTCCGC 
CGATTCCAGA TGATTCCTCT GGACCCCAAA GGGACATCCC AAAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGATCCTGG ACTCGCTGTA 
GGTTATGATG AGTTTAATGC TGTGGACTTC AGTGGCACCT TCTTCATCAA CACCGAAAGG 
GACGATGACT ATGCTGGATT TGTCTTTGGC TACCAGTCCA GCAGCCGCTT TTATGTTGTG 
ATGTGGAAGC AAGTCACCCA GTCCTACTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCGGGCCTTT CTGTGAAAGT TGTAAACTCC ACCACAGGGC CTGGCGAGCA CCTGCGGAAC 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CCCTGTGGCA TGACCCTCGT 
CACATAGGCT GGAAAGATTT CACCGCCTAC AGATGGCGTC TCAGCCACAG GCCAAAGACG 
GGTTTCATTA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGATAAAA CCTATGCTGG TGGTAGACTA GGGTTGTTTG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCTG ACCTGAAATA CGAATGTAGA GATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_011521971.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520273.1 Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60983
Clone ID Related Accession (Same CDS sequence)	XM_011521971.1 , XM_011521971.2
Accession Version	XM_011521971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	thrombospondin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC AATGGATGCC TGTCCAATCC CTGCTTTGCC 
GGCGTGAAGT GTACTAGCTA CCCTGATGGC AGCTGGAAAT GTGGTGCTTG TCCCCCTGGT 
TACAGTGGAA ATGGCATCCA GTGCACAGAT GTTGATGAGT GCAAAGAAGT GCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACCGGTGT GAGAACACGG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCCC CACGCTTCAC CGGCTCACAG CCCTTCGGCC AGGGTGTCGA ACATGCCACG 
GCCAACAAAC AGGTGTGCAA GCCCCGTAAC CCCTGCACGG ATGGGACCCA CGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TATAGCGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCTGGCAA TGGCATCATC TGCGGGGAGG ACACAGACCT GGATGGCTGG 
CCCAATGAGA ACCTGGTGTG CGTGGCCAAT GCGACTTACC ACTGCAAAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATTGG TGATGCCTGT 
GATGATGACG ATGACAATGA TAAAATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCTC AGTATGACTA TGACAGAGAT GATGTGGGAG ACCGCTGTGA CAACTGTCCC 
TACAACCACA ACCCAGATCA GGCAGACACA GACAACAATG GGGAAGGAGA CGCCTGTGCT 
GCAGACATTG ATGGAGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA CGTCTACAAT 
GTGGACCAGA GAGACACTGA TATGGATGGG GTTGGAGATC AGTGTGACAA TTGCCCCTTG 
GAACACAATC CGGATCAGCT GGACTCTGAC TCAGACCGCA TTGGAGATAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA TGGCCACCAG AACAATCTGG ACAACTGTCC CTATGTGCCC 
AATGCCAACC AGGCTGACCA TGACAAAGAT GGCAAGGGAG ATGCCTGTGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACAAGGAC AACTGCAGAC TCGTGCCCAA TCCCGACCAG 
AAGGACTCTG ACGGCGATGG TCGAGGTGAT GCCTGCAAAG ATGATTTTGA CCATGACAGT 
GTGCCAGACA TCGATGACAT CTGTCCTGAG AATGTTGACA TCAGTGAGAC CGATTTCCGC 
CGATTCCAGA TGATTCCTCT GGACCCCAAA GGGACATCCC AAAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGATCCTGG ACTCGCTGTA 
GGTTATGATG AGTTTAATGC TGTGGACTTC AGTGGCACCT TCTTCATCAA CACCGAAAGG 
GACGATGACT ATGCTGGATT TGTCTTTGGC TACCAGTCCA GCAGCCGCTT TTATGTTGTG 
ATGTGGAAGC AAGTCACCCA GTCCTACTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCGGGCCTTT CTGTGAAAGT TGTAAACTCC ACCACAGGGC CTGGCGAGCA CCTGCGGAAC 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CCCTGTGGCA TGACCCTCGT 
CACATAGGCT GGAAAGATTT CACCGCCTAC AGATGGCGTC TCAGCCACAG GCCAAAGACG 
GGTTTCATTA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGATAAAA CCTATGCTGG TGGTAGACTA GGGTTGTTTG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCTG ACCTGAAATA CGAATGTAGA GATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520273.1
CDS	180..3518
Translation	MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPNGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	XM_011521971.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521971.2

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC AATGGATGCC TGTCCAATCC CTGCTTTGCC 
GGCGTGAAGT GTACTAGCTA CCCTGATGGC AGCTGGAAAT GTGGTGCTTG TCCCCCTGGT 
TACAGTGGAA ATGGCATCCA GTGCACAGAT GTTGATGAGT GCAAAGAAGT GCCTGATGCC 
TGCTTCAACC ACAATGGAGA GCACCGGTGT GAGAACACGG ACCCCGGCTA CAACTGCCTG 
CCCTGCCCCC CACGCTTCAC CGGCTCACAG CCCTTCGGCC AGGGTGTCGA ACATGCCACG 
GCCAACAAAC AGGTGTGCAA GCCCCGTAAC CCCTGCACGG ATGGGACCCA CGACTGCAAC 
AAGAACGCCA AGTGCAACTA CCTGGGCCAC TATAGCGACC CCATGTACCG CTGCGAGTGC 
AAGCCTGGCT ACGCTGGCAA TGGCATCATC TGCGGGGAGG ACACAGACCT GGATGGCTGG 
CCCAATGAGA ACCTGGTGTG CGTGGCCAAT GCGACTTACC ACTGCAAAAA GGATAATTGC 
CCCAACCTTC CCAACTCAGG GCAGGAAGAC TATGACAAGG ATGGAATTGG TGATGCCTGT 
GATGATGACG ATGACAATGA TAAAATTCCA GATGACAGGG ACAACTGTCC ATTCCATTAC 
AACCCAGCTC AGTATGACTA TGACAGAGAT GATGTGGGAG ACCGCTGTGA CAACTGTCCC 
TACAACCACA ACCCAGATCA GGCAGACACA GACAACAATG GGGAAGGAGA CGCCTGTGCT 
GCAGACATTG ATGGAGACGG TATCCTCAAT GAACGGGACA ACTGCCAGTA CGTCTACAAT 
GTGGACCAGA GAGACACTGA TATGGATGGG GTTGGAGATC AGTGTGACAA TTGCCCCTTG 
GAACACAATC CGGATCAGCT GGACTCTGAC TCAGACCGCA TTGGAGATAC CTGTGACAAC 
AATCAGGATA TTGATGAAGA TGGCCACCAG AACAATCTGG ACAACTGTCC CTATGTGCCC 
AATGCCAACC AGGCTGACCA TGACAAAGAT GGCAAGGGAG ATGCCTGTGA CCACGATGAT 
GACAACGATG GCATTCCTGA TGACAAGGAC AACTGCAGAC TCGTGCCCAA TCCCGACCAG 
AAGGACTCTG ACGGCGATGG TCGAGGTGAT GCCTGCAAAG ATGATTTTGA CCATGACAGT 
GTGCCAGACA TCGATGACAT CTGTCCTGAG AATGTTGACA TCAGTGAGAC CGATTTCCGC 
CGATTCCAGA TGATTCCTCT GGACCCCAAA GGGACATCCC AAAATGACCC TAACTGGGTT 
GTACGCCATC AGGGTAAAGA ACTCGTCCAG ACTGTCAACT GTGATCCTGG ACTCGCTGTA 
GGTTATGATG AGTTTAATGC TGTGGACTTC AGTGGCACCT TCTTCATCAA CACCGAAAGG 
GACGATGACT ATGCTGGATT TGTCTTTGGC TACCAGTCCA GCAGCCGCTT TTATGTTGTG 
ATGTGGAAGC AAGTCACCCA GTCCTACTGG GACACCAACC CCACGAGGGC TCAGGGATAC 
TCGGGCCTTT CTGTGAAAGT TGTAAACTCC ACCACAGGGC CTGGCGAGCA CCTGCGGAAC 
GCCCTGTGGC ACACAGGAAA CACCCCTGGC CAGGTGCGCA CCCTGTGGCA TGACCCTCGT 
CACATAGGCT GGAAAGATTT CACCGCCTAC AGATGGCGTC TCAGCCACAG GCCAAAGACG 
GGTTTCATTA GAGTGGTGAT GTATGAAGGG AAGAAAATCA TGGCTGACTC AGGACCCATC 
TATGATAAAA CCTATGCTGG TGGTAGACTA GGGTTGTTTG TCTTCTCTCA AGAAATGGTG 
TTCTTCTCTG ACCTGAAATA CGAATGTAGA GATCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( NM_003246.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003237.2 Homo sapiens thrombospondin 1 (THBS1), mRNA.

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CloneID	OHu27122
Clone ID Related Accession (Same CDS sequence)	NM_003246.4 , NM_003246.3 , XM_011521970.1
Accession Version	NM_003246.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Homo sapiens(human)
Product	thrombospondin-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304754.1, BC136470.1, X14787.1, BU676185.1, BM784452.1, BM512145.1, BG115371.1, BC015134.1, AI168683.1, AI147670.1 and AC037198.7. On May 17, 2019 this sequence version replaced NM_003246.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.50755.1, SRR1803615.23668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260356.6/ ENSP00000260356.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003237.2
CDS	180..3692
Translation	MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	NM_003246.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin 1 (THBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003246.4

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( NM_003246.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003237.2 Homo sapiens thrombospondin 1 (THBS1), mRNA.

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CloneID	OHu27122
Clone ID Related Accession (Same CDS sequence)	NM_003246.4 , NM_003246.3 , XM_011521970.1
Accession Version	NM_003246.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551542400000
Organism	Homo sapiens(human)
Product	thrombospondin-1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304754.1, BC136470.1, X14787.1, BU676185.1, BM784452.1, BM512145.1, BG115371.1, BC015134.1, AI168683.1 and AI147670.1. On Mar 17, 2015 this sequence version replaced NM_003246.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.50755.1, X14787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003237.2
CDS	180..3692
Translation	MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	NM_003246.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thrombospondin 1 (THBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003246.3

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

THBS1 ( XM_011521970.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520272.1 Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

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CloneID	OHu27122
Clone ID Related Accession (Same CDS sequence)	NM_003246.4 , NM_003246.3 , XM_011521970.1
Accession Version	XM_011521970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426003200000
Organism	Homo sapiens(Human)
Product	thrombospondin-1 isoform X1
Comment

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011520272.1

Translation

MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	XM_011521970.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens thrombospondin 1 (THBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521970.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT AGGCGTCCTG TTCCTGATGC ATGTGTGTGG CACCAACCGC 
ATTCCAGAGT CTGGCGGAGA CAACAGCGTG TTTGACATCT TTGAACTCAC CGGGGCCGCC 
CGCAAGGGGT CTGGGCGCCG ACTGGTGAAG GGCCCCGACC CTTCCAGCCC AGCTTTCCGC 
ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGATGACA AGTTCCAAGA CCTGGTGGAT 
GCTGTGCGGG CAGAAAAGGG TTTCCTCCTT CTGGCATCCC TGAGGCAGAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCCT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGGTG 
TCCAATGGCA AGGCGGGCAC CCTGGACCTC AGCCTGACCG TCCAAGGAAA GCAGCACGTG 
GTGTCTGTGG AAGAAGCTCT CCTGGCAACC GGCCAGTGGA AGAGCATCAC CCTGTTTGTG 
CAGGAAGACA GGGCCCAGCT GTACATCGAC TGTGAAAAGA TGGAGAATGC TGAGTTGGAC 
GTCCCCATCC AAAGCGTCTT CACCAGAGAC CTGGCCAGCA TCGCCAGACT CCGCATCGCA 
AAGGGGGGCG TCAATGACAA TTTCCAGGGG GTGCTGCAGA ATGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCCTCCTC 
ACCCTTGACA ACAACGTGGT GAATGGTTCC AGCCCTGCCA TCCGCACTAA CTACATTGGC 
CACAAGACAA AGGACTTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAAC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAAT GAGCTGAGGC GGCCTCCCCT ATGCTATCAC 
AACGGAGTTC AGTACAGAAA TAACGAGGAA TGGACTGTTG ATAGCTGCAC TGAGTGTCAC 
TGTCAGAACT CAGTTACCAT CTGCAAAAAG GTGTCCTGCC CCATCATGCC CTGCTCCAAT 
GCCACAGTTC CTGATGGAGA ATGCTGTCCT CGCTGTTGGC CCAGCGACTC TGCGGACGAT 
GGCTGGTCTC CATGGTCCGA GTGGACCTCC TGTTCTACGA GCTGTGGCAA TGGAATTCAG 
CAGCGCGGCC GCTCCTGCGA TAGCCTCAAC AACCGATGTG AGGGCTCCTC GGTCCAGACA 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGATGGTGG CTGGAGCCAC 
TGGTCCCCGT GGTCATCTTG TTCTGTGACA TGTGGTGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGCCC CCAGATGAAC GGGAAACCCT GTGAAGGCGA AGCGCGGGAG 
ACCAAAGCCT GCAAGAAAGA CGCCTGCCCC ATCAATGGAG GCTGGGGTCC TTGGTCACCA 
TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGTCG TCTCTGCAAC 
AACCCCACAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTAACAGA AAACCAGATC 
TGCAACAAGC AGGACTGTCC AATTGATGGA TGCCTGTCCA ATCCCTGCTT TGCCGGCGTG 
AAGTGTACTA GCTACCCTGA TGGCAGCTGG AAATGTGGTG CTTGTCCCCC TGGTTACAGT 
GGAAATGGCA TCCAGTGCAC AGATGTTGAT GAGTGCAAAG AAGTGCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACCG GTGTGAGAAC ACGGACCCCG GCTACAACTG CCTGCCCTGC 
CCCCCACGCT TCACCGGCTC ACAGCCCTTC GGCCAGGGTG TCGAACATGC CACGGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACGGATGGGA CCCACGACTG CAACAAGAAC 
GCCAAGTGCA ACTACCTGGG CCACTATAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT 
GGCTACGCTG GCAATGGCAT CATCTGCGGG GAGGACACAG ACCTGGATGG CTGGCCCAAT 
GAGAACCTGG TGTGCGTGGC CAATGCGACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TTGGTGATGC CTGTGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCTCAGTATG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAACTG TCCCTACAAC 
CACAACCCAG ATCAGGCAGA CACAGACAAC AATGGGGAAG GAGACGCCTG TGCTGCAGAC 
ATTGATGGAG ACGGTATCCT CAATGAACGG GACAACTGCC AGTACGTCTA CAATGTGGAC 
CAGAGAGACA CTGATATGGA TGGGGTTGGA GATCAGTGTG ACAATTGCCC CTTGGAACAC 
AATCCGGATC AGCTGGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA CCAGAACAAT CTGGACAACT GTCCCTATGT GCCCAATGCC 
AACCAGGCTG ACCATGACAA AGATGGCAAG GGAGATGCCT GTGACCACGA TGATGACAAC 
GATGGCATTC CTGATGACAA GGACAACTGC AGACTCGTGC CCAATCCCGA CCAGAAGGAC 
TCTGACGGCG ATGGTCGAGG TGATGCCTGC AAAGATGATT TTGACCATGA CAGTGTGCCA 
GACATCGATG ACATCTGTCC TGAGAATGTT GACATCAGTG AGACCGATTT CCGCCGATTC 
CAGATGATTC CTCTGGACCC CAAAGGGACA TCCCAAAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGATC CTGGACTCGC TGTAGGTTAT 
GATGAGTTTA ATGCTGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA AAGGGACGAT 
GACTATGCTG GATTTGTCTT TGGCTACCAG TCCAGCAGCC GCTTTTATGT TGTGATGTGG 
AAGCAAGTCA CCCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG ATACTCGGGC 
CTTTCTGTGA AAGTTGTAAA CTCCACCACA GGGCCTGGCG AGCACCTGCG GAACGCCCTG 
TGGCACACAG GAAACACCCC TGGCCAGGTG CGCACCCTGT GGCATGACCC TCGTCACATA 
GGCTGGAAAG ATTTCACCGC CTACAGATGG CGTCTCAGCC ACAGGCCAAA GACGGGTTTC 
ATTAGAGTGG TGATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAT 
AAAACCTATG CTGGTGGTAG ACTAGGGTTG TTTGTCTTCT CTCAAGAAAT GGTGTTCTTC 
TCTGACCTGA AATACGAATG TAGAGATCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[The poor prognosis is correlated with the high expression of vascular endothelial growth factor (VEGF) and low expression of thrombospondin 1 (TSP-1) in patients with breast cancer].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology35(9)828-831(2019 Sep)
Huang Q,Huang Y,Li M,Zhang W

The Pathological Significance and Prognostic Roles of Thrombospondin-1, and -2, and 4N1K-peptide in Bladder Cancer.
Anticancer research39(5)2317-2324(2019 May)
Nakamura Y,Miyata Y,Takehara K,Asai A,Mitsunari K,Araki K,Matsuo T,Ohba K,Sakai H

Deciphering the complex role of thrombospondin-1 in glioblastoma development.
Nature communications10(1)1146(2019 03)
Daubon T,Léon C,Clarke K,Andrique L,Salabert L,Darbo E,Pineau R,Guérit S,Maitre M,Dedieu S,Jeanne A,Bailly S,Feige JJ,Miletic H,Rossi M,Bello L,Falciani F,Bjerkvig R,Bikfalvi A

The Increase of pFAK and THBS1 Protein and Gene Expression Levels in Vascular Smooth Muscle Cells by Histamine-treated M1 Macrophages.
ian journal of allergy, asthma, and immunology18(1)72-79(2019 Feb)
Khosravi M,Najafi M,Amirfarhangi A,Karimi M,Fattahi F,Shabani M

Thrombospondin 1 Is Increased in the Aorta and Plasma of Patients With Acute Aortic Dissection.
The Canadian journal of cardiology35(1)42-50(2019 01)
Zeng T,Yuan J,Gan J,Liu Y,Shi L,Lu Z,Xue Y,Xiong R,Huang M,Yang Z,Lin Y,Liu L

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THBS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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