dis3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dis3
Entrez Gene ID	100145718
Full Name	DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
Synonyms	ba555g22.1,dis3p,rrp44
General protein information	Preferred Names DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease Names exosome complex exonuclease RRP44 DIS3 exosome endoribonuclease and 3'-5' exoribonuclease DIS3 mitotic control homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956497.2	XP_012811951.1	exosome complex exonuclease RRP44 isoform X1
NM_001127092.1	NP_001120564.1	exosome complex exonuclease RRP44
XM_012956497.3	XP_012811951.1	exosome complex exonuclease RRP44 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DIS3	XP_542610.3
Magnaporthe oryzae (rice blast fungus)	MGG_00193	XP_003718924.1
Xenopus tropicalis (tropical clawed frog)	dis3	NP_001120564.1
Arabidopsis thaliana (thale cress)	EMB2763	NP_001189541.1
Eremothecium gossypii	AGOS_AEL031C	NP_984830.1
Neurospora crassa	NCU03065	XP_963787.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005191	XP_314089.3
Pan troglodytes (chimpanzee)	DIS3	XP_509678.2
Bos taurus (cattle)	DIS3	XP_002691932.2
Mus musculus (house mouse)	Dis3	NP_082591.2
Gallus gallus (chicken)	DIS3	XP_417016.3
Caenorhabditis elegans (roundworm)	dis-3	NP_501835.2
Oryza sativa (rice)	Os03g0129200	NP_001048846.1
Saccharomyces cerevisiae (baker's yeast)	DIS3	NP_014621.1
Kluyveromyces lactis	KLLA0A10835g	XP_451472.1
Homo sapiens (human)	DIS3	NP_055768.3
Macaca mulatta (Rhesus monkey)	DIS3	XP_001083933.1
Rattus norvegicus (Norway rat)	Dis3	NP_001120955.1
Drosophila melanogaster (fruit fly)	Dis3	NP_001262910.1
Schizosaccharomyces pombe (fission yeast)	dis3	NP_596653.1
Danio rerio (zebrafish)	dis3	XP_001336850.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dis3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dis3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa02117	NM_001127092.1 Latest version!	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa02117	XM_012956497.3 Latest version!	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa02117	XM_012956497.2	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dis3 ( NM_001127092.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120564.1 Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), mRNA.

Price & Availability↑

CloneID	OXa02117
Clone ID Related Accession (Same CDS sequence)	XM_012956497.2 , NM_001127092.1 , XM_012956497.3
Accession Version	NM_001127092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	exosome complex exonuclease RRP44
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161523.1. ##Evidence-Data-START## Transcript exon combination :: BC161523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120564.1
CDS	171..3026
Translation	MLKSKTFMKKTRSGGILKIVREHYLRDDITCGCKGCDECQQERAILELEPILESSLCTTAHYLLPDTNVLLHQIDILEDPILKNVILLQTVLQEVRSRSAPVYKRIKDLLHNPDKCFYAFTNEHHRETYLEQEQGENANDRNDRAIRTATKWYNKHLKKSPKMADVQVILITNDRRNKEKAVEEGIVAYTCEEYIKSLTANPELVDRLACVNDEKNEVESGRLIFAEHLPLSKLQQGIKSGAYLQGTFRASRDNYLEATVWVHGDSAENKEIIVQGLRNLNRAMHEDIVAVQLLDKENWVTMSSVVLQDEGDSDDIESVEEKEKMLKTAVNSVKKPSGQVVGIIKRNWRPYCGMLSKSQIKESTRHLFTPAERRIPRIRIETRQASTLEGQRIIVAVDGWPRNSRYPNGHFVKSLGTAGDKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWTITPEDMKNRIDLRHLYVCSVDPPGCTDIDDALHCRELENGTLEVGVHIADVSHFIRPGNALDQESSNRGTTVYLCDKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEVDENANILNTKFTKSVINSKASLTYAEAQMRIDSPNMNDEITQSLRLLNKLAKILKKRRIDNGALTLSSPEVRFHMDSETHDPIDLQTKELKETNSMVEEFMLLANISVAQKIYDEFSEYALLRKHPSPPPANYDILVKAAKSKNLDIKTDSAKALADSLDKAESPGFPYMNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAVGADSTYPDLTDKHKIADICKNINFRHKMAQYSQRASVAFHTQLFFKNRGIVNEEGFILFVRKNAIVVLIPKFGLEGTVFFEEKDKKKTNLVFNDEVPFLKVENTTFHMFDKVKVKISLDASNIQHQKIRMALVEPEIPGVTSHEDIATNTNGEPVPKKSKS

Target ORF information:

RefSeq Version	NM_001127092.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127092.1

ORF Insert Sequence:

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dis3 ( XM_012956497.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811951.1 Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa02117
Clone ID Related Accession (Same CDS sequence)	XM_012956497.2 , NM_001127092.1 , XM_012956497.3
Accession Version	XM_012956497.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	exosome complex exonuclease RRP44 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956497.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811951.1
CDS	199..3054
Translation	MLKSKTFMKKTRSGGILKIVREHYLRDDITCGCKGCDECQQERAILELEPILESSLCTTAHYLLPDTNVLLHQIDILEDPILKNVILLQTVLQEVRSRSAPVYKRIKDLLHNPDKCFYAFTNEHHRETYLEQEQGENANDRNDRAIRTATKWYNKHLKKSPKMADVQVILITNDRRNKEKAVEEGIVAYTCEEYIKSLTANPELVDRLACVNDEKNEVESGRLIFAEHLPLSKLQQGIKSGAYLQGTFRASRDNYLEATVWVHGDSAENKEIIVQGLRNLNRAMHEDIVAVQLLDKENWVTMSSVVLQDEGDSDDIESVEEKEKMLKTAVNSVKKPSGQVVGIIKRNWRPYCGMLSKSQIKESTRHLFTPAERRIPRIRIETRQASTLEGQRIIVAVDGWPRNSRYPNGHFVKSLGTAGDKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWTITPEDMKNRIDLRHLYVCSVDPPGCTDIDDALHCRELENGTLEVGVHIADVSHFIRPGNALDQESSNRGTTVYLCDKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEVDENANILNTKFTKSVINSKASLTYAEAQMRIDSPNMNDEITQSLRLLNKLAKILKKRRIDNGALTLSSPEVRFHMDSETHDPIDLQTKELKETNSMVEEFMLLANISVAQKIYDEFSEYALLRKHPSPPPANYDILVKAAKSKNLDIKTDSAKALADSLDKAESPGFPYMNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAVGADSTYPDLTDKHKIADICKNINFRHKMAQYSQRASVAFHTQLFFKNRGIVNEEGFILFVRKNAIVVLIPKFGLEGTVFFEEKDKKKTNLVFNDEVPFLKVENTTFHMFDKVKVKISLDASNIQHQKIRMALVEPEIPGVTSHEDIATNTNGEPVPKKSKS

Target ORF information:

RefSeq Version	XM_012956497.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956497.2

ORF Insert Sequence:

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dis3 ( XM_012956497.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811951.1 Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.

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CloneID	OXa02117
Clone ID Related Accession (Same CDS sequence)	XM_012956497.2 , NM_001127092.1 , XM_012956497.3
Accession Version	XM_012956497.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	exosome complex exonuclease RRP44 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811951.1
CDS	214..3069
Translation	MLKSKTFMKKTRSGGILKIVREHYLRDDITCGCKGCDECQQERAILELEPILESSLCTTAHYLLPDTNVLLHQIDILEDPILKNVILLQTVLQEVRSRSAPVYKRIKDLLHNPDKCFYAFTNEHHRETYLEQEQGENANDRNDRAIRTATKWYNKHLKKSPKMADVQVILITNDRRNKEKAVEEGIVAYTCEEYIKSLTANPELVDRLACVNDEKNEVESGRLIFAEHLPLSKLQQGIKSGAYLQGTFRASRDNYLEATVWVHGDSAENKEIIVQGLRNLNRAMHEDIVAVQLLDKENWVTMSSVVLQDEGDSDDIESVEEKEKMLKTAVNSVKKPSGQVVGIIKRNWRPYCGMLSKSQIKESTRHLFTPAERRIPRIRIETRQASTLEGQRIIVAVDGWPRNSRYPNGHFVKSLGTAGDKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWTITPEDMKNRIDLRHLYVCSVDPPGCTDIDDALHCRELENGTLEVGVHIADVSHFIRPGNALDQESSNRGTTVYLCDKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEVDENANILNTKFTKSVINSKASLTYAEAQMRIDSPNMNDEITQSLRLLNKLAKILKKRRIDNGALTLSSPEVRFHMDSETHDPIDLQTKELKETNSMVEEFMLLANISVAQKIYDEFSEYALLRKHPSPPPANYDILVKAAKSKNLDIKTDSAKALADSLDKAESPGFPYMNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAVGADSTYPDLTDKHKIADICKNINFRHKMAQYSQRASVAFHTQLFFKNRGIVNEEGFILFVRKNAIVVLIPKFGLEGTVFFEEKDKKKTNLVFNDEVPFLKVENTTFHMFDKVKVKISLDASNIQHQKIRMALVEPEIPGVTSHEDIATNTNGEPVPKKSKS

Target ORF information:

RefSeq Version	XM_012956497.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (dis3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956497.3

ORF Insert Sequence:

ATGTTGAAGT CAAAAACGTT TATGAAGAAA ACCCGTTCTG GTGGCATTTT AAAGATTGTC 
AGAGAACATT ATCTGCGTGA TGATATTACC TGCGGTTGCA AAGGATGTGA CGAGTGCCAG 
CAGGAGAGAG CAATCTTGGA GCTTGAGCCC ATTTTAGAGA GCTCACTTTG CACAACAGCG 
CACTATTTGC TACCAGACAC CAATGTACTA CTCCATCAGA TTGATATCCT TGAGGACCCC 
ATCCTTAAAA ATGTGATTTT ACTGCAGACT GTTCTACAGG AAGTAAGGAG TCGTAGTGCT 
CCAGTTTACA AAAGAATTAA AGATCTCTTA CACAATCCTG ATAAGTGTTT CTATGCATTC 
ACCAATGAGC ATCATAGAGA AACCTACTTA GAACAGGAGC AGGGAGAAAA TGCAAATGAT 
CGCAATGACA GAGCCATTAG AACTGCGACT AAATGGTACA ATAAGCACTT AAAAAAATCT 
CCAAAAATGG CTGATGTCCA GGTGATTTTA ATAACAAATG ACAGACGAAA CAAAGAGAAG 
GCAGTGGAAG AAGGAATTGT GGCATATACA TGTGAAGAAT ACATTAAAAG TTTGACTGCT 
AACCCTGAAC TTGTTGACCG ACTTGCCTGT GTTAATGATG AAAAGAATGA AGTAGAAAGT 
GGAAGGTTAA TTTTTGCCGA ACACCTTCCG TTGTCAAAGT TGCAACAAGG TATAAAGTCT 
GGTGCATACC TGCAAGGAAC CTTCCGTGCA AGCAGGGACA ACTACCTGGA GGCCACAGTA 
TGGGTTCATG GGGATTCAGC AGAAAACAAA GAGATCATTG TACAAGGACT GAGGAATTTA 
AACAGAGCCA TGCATGAAGA TATTGTCGCT GTGCAGTTGC TGGACAAAGA GAACTGGGTG 
ACTATGTCAT CTGTCGTACT GCAGGATGAA GGTGATAGTG ATGATATTGA GTCTGTAGAG 
GAAAAAGAAA AAATGCTGAA GACTGCTGTT AATAGCGTAA AAAAACCTTC TGGACAAGTA 
GTGGGGATTA TAAAGAGGAA CTGGAGACCA TATTGCGGCA TGCTGTCCAA ATCACAGATT 
AAGGAGTCCA CGAGGCATTT ATTCACGCCT GCAGAAAGGA GAATACCACG AATCCGTATA 
GAGACAAGAC AGGCGTCTAC TTTAGAAGGA CAGAGGATCA TTGTTGCTGT TGACGGCTGG 
CCTAGGAACT CCAGATATCC TAATGGACAC TTTGTAAAGA GCCTGGGAAC TGCTGGTGAT 
AAAGAAACAG AGACAGAAGT GTTACTCCTG GAACATGATG TTCCTCACCA ACCATTCTCC 
CAGGCTGTTC TCAGTTTTCT TCCCAAAATG CCGTGGACCA TCACACCAGA GGATATGAAG 
AACAGAATTG ATCTGAGGCA TCTTTACGTG TGCAGTGTTG ATCCTCCTGG CTGCACTGAT 
ATTGATGATG CACTACATTG TAGAGAGTTG GAAAATGGAA CTTTAGAGGT CGGAGTCCAC 
ATCGCAGATG TCAGCCATTT TATTAGGCCT GGAAATGCTC TGGACCAGGA ATCTTCTAAC 
AGAGGAACAA CTGTATATCT CTGTGACAAA AGAATTGACA TGGTTCCAGA ACTGCTGAGC 
TCAAATCTCT GTTCGTTAAG GTCTAACGTA GACAGATTGG CATTTTCCTG TATTTGGGAA 
GTGGATGAAA ACGCTAATAT TTTAAATACA AAGTTTACTA AAAGTGTCAT TAATTCAAAG 
GCTTCCCTTA CATATGCAGA AGCACAGATG AGAATTGATT CACCAAACAT GAACGATGAG 
ATCACTCAGA GTCTTCGATT GCTAAATAAA CTAGCTAAGA TTTTGAAGAA ACGGCGAATA 
GATAATGGTG CTTTAACGCT GTCTTCGCCT GAGGTTCGAT TCCACATGGA CAGTGAAACC 
CATGATCCCA TAGACCTGCA AACTAAGGAA CTGAAGGAAA CCAATTCTAT GGTTGAAGAA 
TTCATGTTGT TGGCCAATAT TTCTGTGGCA CAGAAGATTT ACGATGAGTT CTCAGAATAT 
GCCCTGCTTC GCAAACATCC ATCTCCTCCT CCTGCTAATT ATGATATTCT GGTTAAAGCA 
GCCAAATCTA AGAATTTGGA CATTAAGACT GACTCTGCCA AAGCTCTAGC TGATTCTTTA 
GACAAAGCTG AATCACCGGG GTTTCCTTAC ATGAACACTC TATTGAGAAT TCTGGCTACA 
CGATGCATGA TGCAGGCAGT TTATTTCTGC TCTGGAATGG ATAACGATTT CCATCACTAT 
GGTCTAGCTT CTCCTATTTA CACCCACTTC ACTTCTCCCA TCAGAAGGTA TGCTGATATT 
ATAGTACATA GACTTCTTGC TGTAGCCGTG GGAGCTGATT CCACTTACCC AGATCTTACA 
GACAAACACA AAATAGCAGA CATCTGCAAA AATATCAACT TCCGGCATAA AATGGCGCAG 
TATTCGCAAA GGGCATCTGT TGCGTTTCAT ACTCAGTTGT TTTTCAAAAA CAGAGGAATA 
GTAAATGAAG AGGGCTTTAT CCTCTTTGTT CGAAAGAATG CAATAGTGGT TCTAATTCCA 
AAATTTGGCT TGGAAGGAAC AGTCTTTTTT GAAGAAAAGG ACAAGAAAAA AACAAATTTG 
GTTTTCAATG ATGAGGTTCC CTTTTTGAAA GTAGAAAATA CAACTTTTCA TATGTTTGAC 
AAAGTAAAAG TAAAAATCTC ATTAGATGCA TCCAACATCC AGCATCAGAA GATCCGCATG 
GCTCTTGTTG AACCCGAGAT ACCTGGAGTG ACATCACATG AAGATATTGC AACAAACACA 
AATGGGGAGC CAGTTCCCAA AAAGAGTAAA TCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dis3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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