Dis3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dis3
Entrez Gene ID	306103
Full Name	DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
Synonyms	RGD1304646
General protein information	Preferred Names DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease Names exosome complex exonuclease RRP44 DIS3 exosome endoribonuclease and 3'-5' exoribonuclease DIS3 mitotic control homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770869.2	XP_008769091.1	exosome complex exonuclease RRP44 isoform X2
XM_017599715.1	XP_017455204.1	exosome complex exonuclease RRP44 isoform X1
NM_001127483.1	NP_001120955.1	exosome complex exonuclease RRP44

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DIS3	XP_542610.3
Magnaporthe oryzae (rice blast fungus)	MGG_00193	XP_003718924.1
Xenopus tropicalis (tropical clawed frog)	dis3	NP_001120564.1
Arabidopsis thaliana (thale cress)	EMB2763	NP_001189541.1
Eremothecium gossypii	AGOS_AEL031C	NP_984830.1
Neurospora crassa	NCU03065	XP_963787.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005191	XP_314089.3
Pan troglodytes (chimpanzee)	DIS3	XP_509678.2
Bos taurus (cattle)	DIS3	XP_002691932.2
Mus musculus (house mouse)	Dis3	NP_082591.2
Gallus gallus (chicken)	DIS3	XP_417016.3
Caenorhabditis elegans (roundworm)	dis-3	NP_501835.2
Oryza sativa (rice)	Os03g0129200	NP_001048846.1
Saccharomyces cerevisiae (baker's yeast)	DIS3	NP_014621.1
Kluyveromyces lactis	KLLA0A10835g	XP_451472.1
Homo sapiens (human)	DIS3	NP_055768.3
Macaca mulatta (Rhesus monkey)	DIS3	XP_001083933.1
Rattus norvegicus (Norway rat)	Dis3	NP_001120955.1
Drosophila melanogaster (fruit fly)	Dis3	NP_001262910.1
Schizosaccharomyces pombe (fission yeast)	dis3	NP_596653.1
Danio rerio (zebrafish)	dis3	XP_001336850.2

Gene Ontology
Bibliography

Related articles in PubMed

An overview of pre-ribosomal RNA processing in eukaryotes.
Henras AK, Plisson-Chastang C, O'Donohue MF, Chakraborty A, Gleizes PE
Wiley interdisciplinary reviews. RNA6(2)225-42(2015 Mar-Apr)

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.
Tomecki R, Kristiansen MS, Lykke-Andersen S, Chlebowski A, Larsen KM, Szczesny RJ, Drazkowska K, Pastula A, Andersen JS, Stepien PP, Dziembowski A, Jensen TH
The EMBO journal29(14)2342-57(2010 Jul)

Dis3-like 1: a novel exoribonuclease associated with the human exosome.
Staals RH, Bronkhorst AW, Schilders G, Slomovic S, Schuster G, Heck AJ, Raijmakers R, Pruijn GJ
The EMBO journal29(14)2358-67(2010 Jul)

Addition of poly(A) and poly(A)-rich tails during RNA degradation in the cytoplasm of human cells.
Slomovic S, Fremder E, Staals RH, Pruijn GJ, Schuster G
Proceedings of the National Academy of Sciences of the United States of America107(16)7407-12(2010 Apr)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dis3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dis3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13752	NM_001127483.1 Latest version!	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa46368	XM_008770869.2 Latest version!	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa58598	XM_017599715.1 Latest version!	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dis3 ( NM_001127483.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001120955.1 Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), mRNA.

Price & Availability↑

CloneID	ORa13752
Clone ID Related Accession (Same CDS sequence)	NM_001127483.1
Accession Version	NM_001127483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	exosome complex exonuclease RRP44
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166824.1. On or before May 22, 2008 this sequence version replaced XM_001073902.1, XM_224449.4. ##Evidence-Data-START## Transcript exon combination :: BC166824.1, FQ210067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTCAAGT CCAAAACATT CTTAAAGAAG ACCCGCGCGG GCGGCGTGGT GAAGATCGTG 
CGCGAGCACT ACCTGCGGGA TGACATCGGC TGCGGCACGC CGGCTTGCTC GGCCTGTGGC 
GCGGCGCACG CGGGCCCGGC CCTGGAGCTG CAGCCCCGGG ACCAGGCGAG CAGCCTCTGC 
CCATGGCCGC ACTACCTCCT GCCGGACACC AATGTGCTGC TGCACCAGAT TGATGTTCTT 
GAGGACCCTG CTATCAGGAA CGTAATTGTG CTTCAGACAG TCCTGCAGGA AGTGAGAAAC 
CGGAGTGCCC CCATATATAA GCGAATCAGG GATGTGACGA ATAACCAGGA GAAGCATTTC 
TATACGTTCA CTAACGAGCA CCATAAAGAA ACCTACATAG AGCAAGAACA GGGGGAGAAT 
GCCAATGACA GAAATGACAG AGCCATTCGA GTAGCAGCAA AGTGGTACAA TGAACATCTA 
AAGATGTCAG CAGAAAATCA GCTACAAGTT ATCCTTATAA CAAACGACAA GAAGAACAAA 
GAAAAGGCTG CAGAAGAAGG AATACCAGCT TTCACATGTG AAGAATATGT AAAGAGCCTG 
ACTGCTAACC CTGAACTTAT AGATCGTCTT GCTTGTTTGT CTGATGAGAT GAATGAAATA 
GAAAGTGGGA AAATAATATT TTCAGAGCAT CTTCCTTTAA GCAAGCTCCA ACAAGGCATA 
AAATCTGGTT CATACCTTCA AGGAACATTC AGAGCTAGCA GGGAAAATTA CTTAGAAGCT 
ACAGTGTGGA TTCATGGAGA CAAAGAAGAT GAAAAAGAGA TACTTATACA AGGACTTAAA 
CATCTAAACA GAGCTATTCA TGAAGATATT GTGGCTGTGG AACTATTGCC CAAGAGTCAG 
TGGGTGGCAC CATCTTCTGT GGTTTTACAT GATGAAGGTC AAAATGAAGA TGATGTGGAG 
AAAGATGAGG AGAGAGAACT CCTGCTTAAG ACTGCTGTAA GTGAAAAAAT GTTGAGGCCT 
ACAGGTAGAG TTGTGGGGAT AATAAAGAGG AATTGGAGAC CGTACTGTGG CATGCTTTCC 
AAGTCTGATA TTAAGGAGTC AAGAAGACAT CTCTTTACAC CTGCTGATAA GAGAATTCCA 
CGAATTCGGA TAGAAACCAG ACAGGCTTCT GCATTAGAAG GACGGAGAAT TATTGTCGCT 
ATTGATGGTT GGCCCAGAAA TTCTAGATAT CCAAATGGAC ACTTTGTAAA AAATTTAGGT 
GATGTTGGAG AGAAAGAGAC GGAAACGGAA GTTTTGCTGC TTGAGCATGA CGTTCCTCAT 
CAGCCCTTTT CCCAGGCTGT TCTTAGCTTC CTGCCGAAGA TGCCCTGGAG CATTACTGAG 
GAGGACATGA AAAACCGAGA AGATCTGAGA CATCTGTGTG TTTGCAGTGT GGATCCTCCA 
GGATGTACTG ACATAGATGA TGCCCTGCAC TGCAGAGAAC TCAGCAATGG AAACTTGGAG 
GTTGGTGTTC ATATTGCGGA TGTTAGCCAT TTTATCAGGC CAGGAAATGC ATTGGATCAA 
GAATCAGCAA GAAGAGGAAC AACTGTTTAT CTTTGTGAAA AGAGGATTGA CATGGTCCCA 
GAGCTGCTCA GCTCCAACCT CTGTTCCTTA AGATCCAACG TGGACAGGTT GGCATTTTCC 
TGTATTTGGG AAATGAATCA TAATGCTGAA ATATTAAAAA CGAGATTTAC CAAAAGTGTC 
ATTAATTCAA AGGCTTCTCT TACATATGCT GAAGCACAGA TGAGAATTGA TTCAGCCGCT 
ATGAATGATG ACATTACCAC TAGTCTCCGT GGACTGAATA AACTAGCTAA AATTCTAAAA 
AGGGGAAGGA TTGAAAAGGG GGCTTTGACA CTCTCTTCTC CAGAAATTCG ATTTCACATG 
GACAGTGAAA CTCACGACCC AATTGACCTG CAGACCAAGG AGCTGAGAGA AACAAATTCC 
ATGGTGGAAG AATTTATGTT ACTCGCTAAT ATTTCTGTTG CAAAAAAAAT TCATGAAGAG 
TTTTCTGAAC ATGCTTTGCT TCGAAAACAT CCAGCCCCAC CTCCCTCCAA TTATGAAGTT 
CTTGTTAAGG CCGCTAAGTC CAAGAATTTG GAAATTCGGA CTGATACAGC CAAATCTTTG 
GCTGACTCTT TGGACCGGGC TGAATGTCCT GATTTCCCAT ACCTGAATAC TCTGTTAAGG 
ATATTGGCCA CTCGCTGTAT GATGCAAGCT GTCTACTTCT GTTCTGGGAT GGATAGTGAC 
TTTCATCACT ATGGCTTAGC CTCCCCAATA TACACACATT TCACTTCTCC TATCAGAAGA 
TATGCAGACA TAATTGTTCA TCGGCTATTA GCTGTGGCGA TTGGGGCTGA TTGTACTTAT 
CCAGAGTTGA CGGACAAACA CAAGCTTTCA GATATATGTA AAAACCTCAA TTTCCGGCAT 
AAAATGGCTC AGTATGCCCA GCGTGCGTCA GTAGCTTTTC ATACACAGTT GTTTTTCAAA 
AGCAAAGGAA TAGTAAGCGA AGAAGCCTAT ATTCTCTTTG TAAGAAAAAA TGCAATTGTA 
GTGTTAATTC CAAAGTATGG CTTAGAAGGT ACAGTTTTTT TTGAAGAAAA AGATAAACCA 
AAGCCACGCC TTACTTATGA TGATGAGATA CCTTCACTCA GAATAGAAGG TACAGTGTTC 
CATGTGTTTG ATAAAGTTAA AGTGAAAATC ACATTAGATT CATCAAATCT TCAACACCAG 
AAAATCCGCA TGGCCCTTGT AGAACCACAG ATACCAGGAA TAAATATGCC TCCTGATGTT 
TCAGACATGG ATCTTAATGG ACCAGGGGGA AAGAAGAGGA AACTTGAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120955.1
CDS	87..2960
Translation	MLKSKTFLKKTRAGGVVKIVREHYLRDDIGCGTPACSACGAAHAGPALELQPRDQASSLCPWPHYLLPDTNVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPIYKRIRDVTNNQEKHFYTFTNEHHKETYIEQEQGENANDRNDRAIRVAAKWYNEHLKMSAENQLQVILITNDKKNKEKAAEEGIPAFTCEEYVKSLTANPELIDRLACLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEDEKEILIQGLKHLNRAIHEDIVAVELLPKSQWVAPSSVVLHDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDALHCRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEMNHNAEILKTRFTKSVINSKASLTYAEAQMRIDSAAMNDDITTSLRGLNKLAKILKRGRIEKGALTLSSPEIRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEVLVKAAKSKNLEIRTDTAKSLADSLDRAECPDFPYLNTLLRILATRCMMQAVYFCSGMDSDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAIGADCTYPELTDKHKLSDICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPKPRLTYDDEIPSLRIEGTVFHVFDKVKVKITLDSSNLQHQKIRMALVEPQIPGINMPPDVSDMDLNGPGGKKRKLEK

Target ORF information:

RefSeq Version	NM_001127483.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127483.1

ORF Insert Sequence:

ATGCTCAAGT CCAAAACATT CTTAAAGAAG ACCCGCGCGG GCGGCGTGGT GAAGATCGTG 
CGCGAGCACT ACCTGCGGGA TGACATCGGC TGCGGCACGC CGGCTTGCTC GGCCTGTGGC 
GCGGCGCACG CGGGCCCGGC CCTGGAGCTG CAGCCCCGGG ACCAGGCGAG CAGCCTCTGC 
CCATGGCCGC ACTACCTCCT GCCGGACACC AATGTGCTGC TGCACCAGAT TGATGTTCTT 
GAGGACCCTG CTATCAGGAA CGTAATTGTG CTTCAGACAG TCCTGCAGGA AGTGAGAAAC 
CGGAGTGCCC CCATATATAA GCGAATCAGG GATGTGACGA ATAACCAGGA GAAGCATTTC 
TATACGTTCA CTAACGAGCA CCATAAAGAA ACCTACATAG AGCAAGAACA GGGGGAGAAT 
GCCAATGACA GAAATGACAG AGCCATTCGA GTAGCAGCAA AGTGGTACAA TGAACATCTA 
AAGATGTCAG CAGAAAATCA GCTACAAGTT ATCCTTATAA CAAACGACAA GAAGAACAAA 
GAAAAGGCTG CAGAAGAAGG AATACCAGCT TTCACATGTG AAGAATATGT AAAGAGCCTG 
ACTGCTAACC CTGAACTTAT AGATCGTCTT GCTTGTTTGT CTGATGAGAT GAATGAAATA 
GAAAGTGGGA AAATAATATT TTCAGAGCAT CTTCCTTTAA GCAAGCTCCA ACAAGGCATA 
AAATCTGGTT CATACCTTCA AGGAACATTC AGAGCTAGCA GGGAAAATTA CTTAGAAGCT 
ACAGTGTGGA TTCATGGAGA CAAAGAAGAT GAAAAAGAGA TACTTATACA AGGACTTAAA 
CATCTAAACA GAGCTATTCA TGAAGATATT GTGGCTGTGG AACTATTGCC CAAGAGTCAG 
TGGGTGGCAC CATCTTCTGT GGTTTTACAT GATGAAGGTC AAAATGAAGA TGATGTGGAG 
AAAGATGAGG AGAGAGAACT CCTGCTTAAG ACTGCTGTAA GTGAAAAAAT GTTGAGGCCT 
ACAGGTAGAG TTGTGGGGAT AATAAAGAGG AATTGGAGAC CGTACTGTGG CATGCTTTCC 
AAGTCTGATA TTAAGGAGTC AAGAAGACAT CTCTTTACAC CTGCTGATAA GAGAATTCCA 
CGAATTCGGA TAGAAACCAG ACAGGCTTCT GCATTAGAAG GACGGAGAAT TATTGTCGCT 
ATTGATGGTT GGCCCAGAAA TTCTAGATAT CCAAATGGAC ACTTTGTAAA AAATTTAGGT 
GATGTTGGAG AGAAAGAGAC GGAAACGGAA GTTTTGCTGC TTGAGCATGA CGTTCCTCAT 
CAGCCCTTTT CCCAGGCTGT TCTTAGCTTC CTGCCGAAGA TGCCCTGGAG CATTACTGAG 
GAGGACATGA AAAACCGAGA AGATCTGAGA CATCTGTGTG TTTGCAGTGT GGATCCTCCA 
GGATGTACTG ACATAGATGA TGCCCTGCAC TGCAGAGAAC TCAGCAATGG AAACTTGGAG 
GTTGGTGTTC ATATTGCGGA TGTTAGCCAT TTTATCAGGC CAGGAAATGC ATTGGATCAA 
GAATCAGCAA GAAGAGGAAC AACTGTTTAT CTTTGTGAAA AGAGGATTGA CATGGTCCCA 
GAGCTGCTCA GCTCCAACCT CTGTTCCTTA AGATCCAACG TGGACAGGTT GGCATTTTCC 
TGTATTTGGG AAATGAATCA TAATGCTGAA ATATTAAAAA CGAGATTTAC CAAAAGTGTC 
ATTAATTCAA AGGCTTCTCT TACATATGCT GAAGCACAGA TGAGAATTGA TTCAGCCGCT 
ATGAATGATG ACATTACCAC TAGTCTCCGT GGACTGAATA AACTAGCTAA AATTCTAAAA 
AGGGGAAGGA TTGAAAAGGG GGCTTTGACA CTCTCTTCTC CAGAAATTCG ATTTCACATG 
GACAGTGAAA CTCACGACCC AATTGACCTG CAGACCAAGG AGCTGAGAGA AACAAATTCC 
ATGGTGGAAG AATTTATGTT ACTCGCTAAT ATTTCTGTTG CAAAAAAAAT TCATGAAGAG 
TTTTCTGAAC ATGCTTTGCT TCGAAAACAT CCAGCCCCAC CTCCCTCCAA TTATGAAGTT 
CTTGTTAAGG CCGCTAAGTC CAAGAATTTG GAAATTCGGA CTGATACAGC CAAATCTTTG 
GCTGACTCTT TGGACCGGGC TGAATGTCCT GATTTCCCAT ACCTGAATAC TCTGTTAAGG 
ATATTGGCCA CTCGCTGTAT GATGCAAGCT GTCTACTTCT GTTCTGGGAT GGATAGTGAC 
TTTCATCACT ATGGCTTAGC CTCCCCAATA TACACACATT TCACTTCTCC TATCAGAAGA 
TATGCAGACA TAATTGTTCA TCGGCTATTA GCTGTGGCGA TTGGGGCTGA TTGTACTTAT 
CCAGAGTTGA CGGACAAACA CAAGCTTTCA GATATATGTA AAAACCTCAA TTTCCGGCAT 
AAAATGGCTC AGTATGCCCA GCGTGCGTCA GTAGCTTTTC ATACACAGTT GTTTTTCAAA 
AGCAAAGGAA TAGTAAGCGA AGAAGCCTAT ATTCTCTTTG TAAGAAAAAA TGCAATTGTA 
GTGTTAATTC CAAAGTATGG CTTAGAAGGT ACAGTTTTTT TTGAAGAAAA AGATAAACCA 
AAGCCACGCC TTACTTATGA TGATGAGATA CCTTCACTCA GAATAGAAGG TACAGTGTTC 
CATGTGTTTG ATAAAGTTAA AGTGAAAATC ACATTAGATT CATCAAATCT TCAACACCAG 
AAAATCCGCA TGGCCCTTGT AGAACCACAG ATACCAGGAA TAAATATGCC TCCTGATGTT 
TCAGACATGG ATCTTAATGG ACCAGGGGGA AAGAAGAGGA AACTTGAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dis3 ( XM_008770869.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769091.1 Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa46368
Clone ID Related Accession (Same CDS sequence)	XM_008770869.2
Accession Version	XM_008770869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	exosome complex exonuclease RRP44 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGT CCAAAACATT CTTAAAGAAG ACCCGCGCGG GCGGCGTGGT GAAGATCGTG 
CGCGAGCACT ACCTGCGGGA TGACATCGGC TGCGGCACGC CGGCTTGCTC GGCCTGTGGC 
GCGGCGCACG CGGGCCCGGC CCTGGAGCTG CAGCCCCGGG ACCAGGCGAG CAGCCTCTGC 
CCATGGCCGC ACTACCTCCT GCCGGACACC AATGTGCTGC TGCACCAGAT TATAAGTGCC 
TGGAAGCCGG GGACCTGGGC TTCTGTGGTC TCCAGATTGC GACCCCCAGG CAGCTTAGAA 
ACCTACATAG AGCAAGAACA GGGGGAGAAT GCCAATGACA GAAATGACAG AGCCATTCGA 
GTAGCAGCAA AGTGGTACAA TGAACATCTA AAGATGTCAG CAGAAAATCA GCTACAAGTT 
ATCCTTATAA CAAACGACAA GAAGAACAAA GAAAAGGCTG CAGAAGAAGG AATACCAGCT 
TTCACATGTG AAGAATATGT AAAGAGCCTG ACTGCTAACC CTGAACTTAT AGATCGTCTT 
GCTTGTTTGT CTGATGAGAT GAATGAAATA GAAAGTGGGA AAATAATATT TTCAGAGCAT 
CTTCCTTTAA GCAAGCTCCA ACAAGGCATA AAATCTGGTT CATACCTTCA AGGAACATTC 
AGAGCTAGCA GGGAAAATTA CTTAGAAGCT ACAGTGTGGA TTCATGGAGA CAAAGAAGAT 
GAAAAAGAGA TACTTATACA AGGACTTAAA CATCTAAACA GAGCTATTCA TGAAGATATT 
GTGGCTGTGG AACTATTGCC CAAGAGTCAG TGGGTGGCAC CATCTTCTGT GGTTTTACAT 
GATGAAGGTC AAAATGAAGA TGATGTGGAG AAAGATGAGG AGAGAGAACT CCTGCTTAAG 
ACTGCTGTAA GTGAAAAAAT GTTGAGGCCT ACAGGTAGAG TTGTGGGGAT AATAAAGAGG 
AATTGGAGAC CGTACTGTGG CATGCTTTCC AAGTCTGATA TTAAGGAGTC AAGAAGACAT 
CTCTTTACAC CTGCTGATAA GAGAATTCCA CGAATTCGGA TAGAAACCAG ACAGGCTTCT 
GCATTAGAAG GACGGAGAAT TATTGTCGCT ATTGATGGTT GGCCCAGAAA TTCTAGATAT 
CCAAATGGAC ACTTTGTAAA AAATTTAGGT GATGTTGGAG AGAAAGAGAC GGAAACGGAA 
GTTTTGCTGC TTGAGCATGA CGTTCCTCAT CAGCCCTTTT CCCAGGCTGT TCTTAGCTTC 
CTGCCGAAGA TGCCCTGGAG CATTACTGAG GAGGACATGA AAAACCGAGA AGATCTGAGA 
CATCTGTGTG TTTGCAGTGT GGATCCTCCA GGATGTACTG ACATAGATGA TGCCCTGCAC 
TGCAGAGAAC TCAGCAATGG AAACTTGGAG GTTGGTGTTC ATATTGCGGA TGTTAGCCAT 
TTTATCAGGC CAGGAAATGC ATTGGATCAA GAATCAGCAA GAAGAGGAAC AACTGTTTAT 
CTTTGTGAAA AGAGGATTGA CATGGTCCCA GAGCTGCTCA GCTCCAACCT CTGTTCCTTA 
AGATCCAACG TGGACAGGTT GGCATTTTCC TGTATTTGGG AAATGAATCA TAATGCTGAA 
ATATTAAAAA CGAGATTTAC CAAAAGTGTC ATTAATTCAA AGGCTTCTCT TACATATGCT 
GAAGCACAGA TGAGAATTGA TTCAGCCGCT ATGAATGATG ACATTACCAC TAGTCTCCGT 
GGACTGAATA AACTAGCTAA AATTCTAAAA AGGGGAAGGA TTGAAAAGGG GGCTTTGACA 
CTCTCTTCTC CAGAAATTCG ATTTCACATG GACAGTGAAA CTCACGACCC AATTGACCTG 
CAGACCAAGG AGCTGAGAGA AACAAATTCC ATGGTGGAAG AATTTATGTT ACTCGCTAAT 
ATTTCTGTTG CAAAAAAAAT TCATGAAGAG TTTTCTGAAC ATGCTTTGCT TCGAAAACAT 
CCAGCCCCAC CTCCCTCCAA TTATGAAGTT CTTGTTAAGG CCGCTAAGTC CAAGAATTTG 
GAAATTCGGA CTGATACAGC CAAATCTTTG GCTGACTCTT TGGACCGGGC TGAATGTCCT 
GATTTCCCAT ACCTGAATAC TCTGTTAAGG ATATTGGCCA CTCGCTGTAT GATGCAAGCT 
GTCTACTTCT GTTCTGGGAT GGATAGTGAC TTTCATCACT ATGGCTTAGC CTCCCCAATA 
TACACACATT TCACTTCTCC TATCAGAAGA TATGCAGACA TAATTGTTCA TCGGCTATTA 
GCTGTGGCGA TTGGGGCTGA TTGTACTTAT CCAGAGTTGA CGGACAAACA CAAGCTTTCA 
GATATATGTA AAAACCTCAA TTTCCGGCAT AAAATGGCTC AGTATGCCCA GCGTGCGTCA 
GTAGCTTTTC ATACACAGTT GTTTTTCAAA AGCAAAGGAA TAGTAAGCGA AGAAGCCTAT 
ATTCTCTTTG TAAGAAAAAA TGCAATTGTA GTGTTAATTC CAAAGTATGG CTTAGAAGGT 
ACAGTTTTTT TTGAAGAAAA AGATAAACCA AAGCCACGCC TTACTTATGA TGATGAGATA 
CCTTCACTCA GAATAGAAGG TACAGTGTTC CATGTGTTTG ATAAAGTTAA AGTGAAAATC 
ACATTAGATT CATCAAATCT TCAACACCAG AAAATCCGCA TGGCCCTTGT AGAACCACAG 
ATACCAGGAA TAAATATGCC TCCTGATGTT TCAGACATGG ATCTTAATGG ACCAGGGGGA 
AAGAAGAGGA AACTTGAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769091.1
CDS	26..2809
Translation	MLKSKTFLKKTRAGGVVKIVREHYLRDDIGCGTPACSACGAAHAGPALELQPRDQASSLCPWPHYLLPDTNVLLHQIISAWKPGTWASVVSRLRPPGSLETYIEQEQGENANDRNDRAIRVAAKWYNEHLKMSAENQLQVILITNDKKNKEKAAEEGIPAFTCEEYVKSLTANPELIDRLACLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEDEKEILIQGLKHLNRAIHEDIVAVELLPKSQWVAPSSVVLHDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDALHCRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEMNHNAEILKTRFTKSVINSKASLTYAEAQMRIDSAAMNDDITTSLRGLNKLAKILKRGRIEKGALTLSSPEIRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEVLVKAAKSKNLEIRTDTAKSLADSLDRAECPDFPYLNTLLRILATRCMMQAVYFCSGMDSDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAIGADCTYPELTDKHKLSDICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPKPRLTYDDEIPSLRIEGTVFHVFDKVKVKITLDSSNLQHQKIRMALVEPQIPGINMPPDVSDMDLNGPGGKKRKLEK

Target ORF information:

RefSeq Version	XM_008770869.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770869.2

ORF Insert Sequence:

ATGCTCAAGT CCAAAACATT CTTAAAGAAG ACCCGCGCGG GCGGCGTGGT GAAGATCGTG 
CGCGAGCACT ACCTGCGGGA TGACATCGGC TGCGGCACGC CGGCTTGCTC GGCCTGTGGC 
GCGGCGCACG CGGGCCCGGC CCTGGAGCTG CAGCCCCGGG ACCAGGCGAG CAGCCTCTGC 
CCATGGCCGC ACTACCTCCT GCCGGACACC AATGTGCTGC TGCACCAGAT TATAAGTGCC 
TGGAAGCCGG GGACCTGGGC TTCTGTGGTC TCCAGATTGC GACCCCCAGG CAGCTTAGAA 
ACCTACATAG AGCAAGAACA GGGGGAGAAT GCCAATGACA GAAATGACAG AGCCATTCGA 
GTAGCAGCAA AGTGGTACAA TGAACATCTA AAGATGTCAG CAGAAAATCA GCTACAAGTT 
ATCCTTATAA CAAACGACAA GAAGAACAAA GAAAAGGCTG CAGAAGAAGG AATACCAGCT 
TTCACATGTG AAGAATATGT AAAGAGCCTG ACTGCTAACC CTGAACTTAT AGATCGTCTT 
GCTTGTTTGT CTGATGAGAT GAATGAAATA GAAAGTGGGA AAATAATATT TTCAGAGCAT 
CTTCCTTTAA GCAAGCTCCA ACAAGGCATA AAATCTGGTT CATACCTTCA AGGAACATTC 
AGAGCTAGCA GGGAAAATTA CTTAGAAGCT ACAGTGTGGA TTCATGGAGA CAAAGAAGAT 
GAAAAAGAGA TACTTATACA AGGACTTAAA CATCTAAACA GAGCTATTCA TGAAGATATT 
GTGGCTGTGG AACTATTGCC CAAGAGTCAG TGGGTGGCAC CATCTTCTGT GGTTTTACAT 
GATGAAGGTC AAAATGAAGA TGATGTGGAG AAAGATGAGG AGAGAGAACT CCTGCTTAAG 
ACTGCTGTAA GTGAAAAAAT GTTGAGGCCT ACAGGTAGAG TTGTGGGGAT AATAAAGAGG 
AATTGGAGAC CGTACTGTGG CATGCTTTCC AAGTCTGATA TTAAGGAGTC AAGAAGACAT 
CTCTTTACAC CTGCTGATAA GAGAATTCCA CGAATTCGGA TAGAAACCAG ACAGGCTTCT 
GCATTAGAAG GACGGAGAAT TATTGTCGCT ATTGATGGTT GGCCCAGAAA TTCTAGATAT 
CCAAATGGAC ACTTTGTAAA AAATTTAGGT GATGTTGGAG AGAAAGAGAC GGAAACGGAA 
GTTTTGCTGC TTGAGCATGA CGTTCCTCAT CAGCCCTTTT CCCAGGCTGT TCTTAGCTTC 
CTGCCGAAGA TGCCCTGGAG CATTACTGAG GAGGACATGA AAAACCGAGA AGATCTGAGA 
CATCTGTGTG TTTGCAGTGT GGATCCTCCA GGATGTACTG ACATAGATGA TGCCCTGCAC 
TGCAGAGAAC TCAGCAATGG AAACTTGGAG GTTGGTGTTC ATATTGCGGA TGTTAGCCAT 
TTTATCAGGC CAGGAAATGC ATTGGATCAA GAATCAGCAA GAAGAGGAAC AACTGTTTAT 
CTTTGTGAAA AGAGGATTGA CATGGTCCCA GAGCTGCTCA GCTCCAACCT CTGTTCCTTA 
AGATCCAACG TGGACAGGTT GGCATTTTCC TGTATTTGGG AAATGAATCA TAATGCTGAA 
ATATTAAAAA CGAGATTTAC CAAAAGTGTC ATTAATTCAA AGGCTTCTCT TACATATGCT 
GAAGCACAGA TGAGAATTGA TTCAGCCGCT ATGAATGATG ACATTACCAC TAGTCTCCGT 
GGACTGAATA AACTAGCTAA AATTCTAAAA AGGGGAAGGA TTGAAAAGGG GGCTTTGACA 
CTCTCTTCTC CAGAAATTCG ATTTCACATG GACAGTGAAA CTCACGACCC AATTGACCTG 
CAGACCAAGG AGCTGAGAGA AACAAATTCC ATGGTGGAAG AATTTATGTT ACTCGCTAAT 
ATTTCTGTTG CAAAAAAAAT TCATGAAGAG TTTTCTGAAC ATGCTTTGCT TCGAAAACAT 
CCAGCCCCAC CTCCCTCCAA TTATGAAGTT CTTGTTAAGG CCGCTAAGTC CAAGAATTTG 
GAAATTCGGA CTGATACAGC CAAATCTTTG GCTGACTCTT TGGACCGGGC TGAATGTCCT 
GATTTCCCAT ACCTGAATAC TCTGTTAAGG ATATTGGCCA CTCGCTGTAT GATGCAAGCT 
GTCTACTTCT GTTCTGGGAT GGATAGTGAC TTTCATCACT ATGGCTTAGC CTCCCCAATA 
TACACACATT TCACTTCTCC TATCAGAAGA TATGCAGACA TAATTGTTCA TCGGCTATTA 
GCTGTGGCGA TTGGGGCTGA TTGTACTTAT CCAGAGTTGA CGGACAAACA CAAGCTTTCA 
GATATATGTA AAAACCTCAA TTTCCGGCAT AAAATGGCTC AGTATGCCCA GCGTGCGTCA 
GTAGCTTTTC ATACACAGTT GTTTTTCAAA AGCAAAGGAA TAGTAAGCGA AGAAGCCTAT 
ATTCTCTTTG TAAGAAAAAA TGCAATTGTA GTGTTAATTC CAAAGTATGG CTTAGAAGGT 
ACAGTTTTTT TTGAAGAAAA AGATAAACCA AAGCCACGCC TTACTTATGA TGATGAGATA 
CCTTCACTCA GAATAGAAGG TACAGTGTTC CATGTGTTTG ATAAAGTTAA AGTGAAAATC 
ACATTAGATT CATCAAATCT TCAACACCAG AAAATCCGCA TGGCCCTTGT AGAACCACAG 
ATACCAGGAA TAAATATGCC TCCTGATGTT TCAGACATGG ATCTTAATGG ACCAGGGGGA 
AAGAAGAGGA AACTTGAAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dis3 ( XM_017599715.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455204.1 Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X1, mRNA.

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CloneID	ORa58598
Clone ID Related Accession (Same CDS sequence)	XM_017599715.1
Accession Version	XM_017599715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	exosome complex exonuclease RRP44 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAC TACCTCCTGC CGGACACCAA TGTGCTGCTG CACCAGATTA TAAGTGCCTG 
GAAGCCGGGG ACCTGGGCTT CTGTGGTCTC CAGATTGCGA CCCCCAGGCA GCTTATTGAT 
GTTCTTGAGG ACCCTGCTAT CAGGAACGTA ATTGTGCTTC AGACAGTCCT GCAGGAAGTG 
AGAAACCGGA GTGCCCCCAT ATATAAGCGA ATCAGGGATG TGACGAATAA CCAGGAGAAG 
CATTTCTATA CGTTCACTAA CGAGCACCAT AAAGAAACCT ACATAGAGCA AGAACAGGGG 
GAGAATGCCA ATGACAGAAA TGACAGAGCC ATTCGAGTAG CAGCAAAGTG GTACAATGAA 
CATCTAAAGA TGTCAGCAGA AAATCAGCTA CAAGTTATCC TTATAACAAA CGACAAGAAG 
AACAAAGAAA AGGCTGCAGA AGAAGGAATA CCAGCTTTCA CATGTGAAGA ATATGTAAAG 
AGCCTGACTG CTAACCCTGA ACTTATAGAT CGTCTTGCTT GTTTGTCTGA TGAGATGAAT 
GAAATAGAAA GTGGGAAAAT AATATTTTCA GAGCATCTTC CTTTAAGCAA GCTCCAACAA 
GGCATAAAAT CTGGTTCATA CCTTCAAGGA ACATTCAGAG CTAGCAGGGA AAATTACTTA 
GAAGCTACAG TGTGGATTCA TGGAGACAAA GAAGATGAAA AAGAGATACT TATACAAGGA 
CTTAAACATC TAAACAGAGC TATTCATGAA GATATTGTGG CTGTGGAACT ATTGCCCAAG 
AGTCAGTGGG TGGCACCATC TTCTGTGGTT TTACATGATG AAGGTCAAAA TGAAGATGAT 
GTGGAGAAAG ATGAGGAGAG AGAACTCCTG CTTAAGACTG CTGTAAGTGA AAAAATGTTG 
AGGCCTACAG GTAGAGTTGT GGGGATAATA AAGAGGAATT GGAGACCGTA CTGTGGCATG 
CTTTCCAAGT CTGATATTAA GGAGTCAAGA AGACATCTCT TTACACCTGC TGATAAGAGA 
ATTCCACGAA TTCGGATAGA AACCAGACAG GCTTCTGCAT TAGAAGGACG GAGAATTATT 
GTCGCTATTG ATGGTTGGCC CAGAAATTCT AGATATCCAA ATGGACACTT TGTAAAAAAT 
TTAGGTGATG TTGGAGAGAA AGAGACGGAA ACGGAAGTTT TGCTGCTTGA GCATGACGTT 
CCTCATCAGC CCTTTTCCCA GGCTGTTCTT AGCTTCCTGC CGAAGATGCC CTGGAGCATT 
ACTGAGGAGG ACATGAAAAA CCGAGAAGAT CTGAGACATC TGTGTGTTTG CAGTGTGGAT 
CCTCCAGGAT GTACTGACAT AGATGATGCC CTGCACTGCA GAGAACTCAG CAATGGAAAC 
TTGGAGGTTG GTGTTCATAT TGCGGATGTT AGCCATTTTA TCAGGCCAGG AAATGCATTG 
GATCAAGAAT CAGCAAGAAG AGGAACAACT GTTTATCTTT GTGAAAAGAG GATTGACATG 
GTCCCAGAGC TGCTCAGCTC CAACCTCTGT TCCTTAAGAT CCAACGTGGA CAGGTTGGCA 
TTTTCCTGTA TTTGGGAAAT GAATCATAAT GCTGAAATAT TAAAAACGAG ATTTACCAAA 
AGTGTCATTA ATTCAAAGGC TTCTCTTACA TATGCTGAAG CACAGATGAG AATTGATTCA 
GCCGCTATGA ATGATGACAT TACCACTAGT CTCCGTGGAC TGAATAAACT AGCTAAAATT 
CTAAAAAGGG GAAGGATTGA AAAGGGGGCT TTGACACTCT CTTCTCCAGA AATTCGATTT 
CACATGGACA GTGAAACTCA CGACCCAATT GACCTGCAGA CCAAGGAGCT GAGAGAAACA 
AATTCCATGG TGGAAGAATT TATGTTACTC GCTAATATTT CTGTTGCAAA AAAAATTCAT 
GAAGAGTTTT CTGAACATGC TTTGCTTCGA AAACATCCAG CCCCACCTCC CTCCAATTAT 
GAAGTTCTTG TTAAGGCCGC TAAGTCCAAG AATTTGGAAA TTCGGACTGA TACAGCCAAA 
TCTTTGGCTG ACTCTTTGGA CCGGGCTGAA TGTCCTGATT TCCCATACCT GAATACTCTG 
TTAAGGATAT TGGCCACTCG CTGTATGATG CAAGCTGTCT ACTTCTGTTC TGGGATGGAT 
AGTGACTTTC ATCACTATGG CTTAGCCTCC CCAATATACA CACATTTCAC TTCTCCTATC 
AGAAGATATG CAGACATAAT TGTTCATCGG CTATTAGCTG TGGCGATTGG GGCTGATTGT 
ACTTATCCAG AGTTGACGGA CAAACACAAG CTTTCAGATA TATGTAAAAA CCTCAATTTC 
CGGCATAAAA TGGCTCAGTA TGCCCAGCGT GCGTCAGTAG CTTTTCATAC ACAGTTGTTT 
TTCAAAAGCA AAGGAATAGT AAGCGAAGAA GCCTATATTC TCTTTGTAAG AAAAAATGCA 
ATTGTAGTGT TAATTCCAAA GTATGGCTTA GAAGGTACAG TTTTTTTTGA AGAAAAAGAT 
AAACCAAAGC CACGCCTTAC TTATGATGAT GAGATACCTT CACTCAGAAT AGAAGGTACA 
GTGTTCCATG TGTTTGATAA AGTTAAAGTG AAAATCACAT TAGATTCATC AAATCTTCAA 
CACCAGAAAA TCCGCATGGC CCTTGTAGAA CCACAGATAC CAGGAATAAA TATGCCTCCT 
GATGTTTCAG ACATGGATCT TAATGGACCA GGGGGAAAGA AGAGGAAACT TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455204.1
CDS	91..2850
Translation	MAALPPAGHQCAAAPDYKCLEAGDLGFCGLQIATPRQLIDVLEDPAIRNVIVLQTVLQEVRNRSAPIYKRIRDVTNNQEKHFYTFTNEHHKETYIEQEQGENANDRNDRAIRVAAKWYNEHLKMSAENQLQVILITNDKKNKEKAAEEGIPAFTCEEYVKSLTANPELIDRLACLSDEMNEIESGKIIFSEHLPLSKLQQGIKSGSYLQGTFRASRENYLEATVWIHGDKEDEKEILIQGLKHLNRAIHEDIVAVELLPKSQWVAPSSVVLHDEGQNEDDVEKDEERELLLKTAVSEKMLRPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASALEGRRIIVAIDGWPRNSRYPNGHFVKNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEEDMKNREDLRHLCVCSVDPPGCTDIDDALHCRELSNGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLRSNVDRLAFSCIWEMNHNAEILKTRFTKSVINSKASLTYAEAQMRIDSAAMNDDITTSLRGLNKLAKILKRGRIEKGALTLSSPEIRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEVLVKAAKSKNLEIRTDTAKSLADSLDRAECPDFPYLNTLLRILATRCMMQAVYFCSGMDSDFHHYGLASPIYTHFTSPIRRYADIIVHRLLAVAIGADCTYPELTDKHKLSDICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPKPRLTYDDEIPSLRIEGTVFHVFDKVKVKITLDSSNLQHQKIRMALVEPQIPGINMPPDVSDMDLNGPGGKKRKLEK

Target ORF information:

RefSeq Version	XM_017599715.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease (Dis3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599715.1

ORF Insert Sequence:

ATGGCCGCAC TACCTCCTGC CGGACACCAA TGTGCTGCTG CACCAGATTA TAAGTGCCTG 
GAAGCCGGGG ACCTGGGCTT CTGTGGTCTC CAGATTGCGA CCCCCAGGCA GCTTATTGAT 
GTTCTTGAGG ACCCTGCTAT CAGGAACGTA ATTGTGCTTC AGACAGTCCT GCAGGAAGTG 
AGAAACCGGA GTGCCCCCAT ATATAAGCGA ATCAGGGATG TGACGAATAA CCAGGAGAAG 
CATTTCTATA CGTTCACTAA CGAGCACCAT AAAGAAACCT ACATAGAGCA AGAACAGGGG 
GAGAATGCCA ATGACAGAAA TGACAGAGCC ATTCGAGTAG CAGCAAAGTG GTACAATGAA 
CATCTAAAGA TGTCAGCAGA AAATCAGCTA CAAGTTATCC TTATAACAAA CGACAAGAAG 
AACAAAGAAA AGGCTGCAGA AGAAGGAATA CCAGCTTTCA CATGTGAAGA ATATGTAAAG 
AGCCTGACTG CTAACCCTGA ACTTATAGAT CGTCTTGCTT GTTTGTCTGA TGAGATGAAT 
GAAATAGAAA GTGGGAAAAT AATATTTTCA GAGCATCTTC CTTTAAGCAA GCTCCAACAA 
GGCATAAAAT CTGGTTCATA CCTTCAAGGA ACATTCAGAG CTAGCAGGGA AAATTACTTA 
GAAGCTACAG TGTGGATTCA TGGAGACAAA GAAGATGAAA AAGAGATACT TATACAAGGA 
CTTAAACATC TAAACAGAGC TATTCATGAA GATATTGTGG CTGTGGAACT ATTGCCCAAG 
AGTCAGTGGG TGGCACCATC TTCTGTGGTT TTACATGATG AAGGTCAAAA TGAAGATGAT 
GTGGAGAAAG ATGAGGAGAG AGAACTCCTG CTTAAGACTG CTGTAAGTGA AAAAATGTTG 
AGGCCTACAG GTAGAGTTGT GGGGATAATA AAGAGGAATT GGAGACCGTA CTGTGGCATG 
CTTTCCAAGT CTGATATTAA GGAGTCAAGA AGACATCTCT TTACACCTGC TGATAAGAGA 
ATTCCACGAA TTCGGATAGA AACCAGACAG GCTTCTGCAT TAGAAGGACG GAGAATTATT 
GTCGCTATTG ATGGTTGGCC CAGAAATTCT AGATATCCAA ATGGACACTT TGTAAAAAAT 
TTAGGTGATG TTGGAGAGAA AGAGACGGAA ACGGAAGTTT TGCTGCTTGA GCATGACGTT 
CCTCATCAGC CCTTTTCCCA GGCTGTTCTT AGCTTCCTGC CGAAGATGCC CTGGAGCATT 
ACTGAGGAGG ACATGAAAAA CCGAGAAGAT CTGAGACATC TGTGTGTTTG CAGTGTGGAT 
CCTCCAGGAT GTACTGACAT AGATGATGCC CTGCACTGCA GAGAACTCAG CAATGGAAAC 
TTGGAGGTTG GTGTTCATAT TGCGGATGTT AGCCATTTTA TCAGGCCAGG AAATGCATTG 
GATCAAGAAT CAGCAAGAAG AGGAACAACT GTTTATCTTT GTGAAAAGAG GATTGACATG 
GTCCCAGAGC TGCTCAGCTC CAACCTCTGT TCCTTAAGAT CCAACGTGGA CAGGTTGGCA 
TTTTCCTGTA TTTGGGAAAT GAATCATAAT GCTGAAATAT TAAAAACGAG ATTTACCAAA 
AGTGTCATTA ATTCAAAGGC TTCTCTTACA TATGCTGAAG CACAGATGAG AATTGATTCA 
GCCGCTATGA ATGATGACAT TACCACTAGT CTCCGTGGAC TGAATAAACT AGCTAAAATT 
CTAAAAAGGG GAAGGATTGA AAAGGGGGCT TTGACACTCT CTTCTCCAGA AATTCGATTT 
CACATGGACA GTGAAACTCA CGACCCAATT GACCTGCAGA CCAAGGAGCT GAGAGAAACA 
AATTCCATGG TGGAAGAATT TATGTTACTC GCTAATATTT CTGTTGCAAA AAAAATTCAT 
GAAGAGTTTT CTGAACATGC TTTGCTTCGA AAACATCCAG CCCCACCTCC CTCCAATTAT 
GAAGTTCTTG TTAAGGCCGC TAAGTCCAAG AATTTGGAAA TTCGGACTGA TACAGCCAAA 
TCTTTGGCTG ACTCTTTGGA CCGGGCTGAA TGTCCTGATT TCCCATACCT GAATACTCTG 
TTAAGGATAT TGGCCACTCG CTGTATGATG CAAGCTGTCT ACTTCTGTTC TGGGATGGAT 
AGTGACTTTC ATCACTATGG CTTAGCCTCC CCAATATACA CACATTTCAC TTCTCCTATC 
AGAAGATATG CAGACATAAT TGTTCATCGG CTATTAGCTG TGGCGATTGG GGCTGATTGT 
ACTTATCCAG AGTTGACGGA CAAACACAAG CTTTCAGATA TATGTAAAAA CCTCAATTTC 
CGGCATAAAA TGGCTCAGTA TGCCCAGCGT GCGTCAGTAG CTTTTCATAC ACAGTTGTTT 
TTCAAAAGCA AAGGAATAGT AAGCGAAGAA GCCTATATTC TCTTTGTAAG AAAAAATGCA 
ATTGTAGTGT TAATTCCAAA GTATGGCTTA GAAGGTACAG TTTTTTTTGA AGAAAAAGAT 
AAACCAAAGC CACGCCTTAC TTATGATGAT GAGATACCTT CACTCAGAAT AGAAGGTACA 
GTGTTCCATG TGTTTGATAA AGTTAAAGTG AAAATCACAT TAGATTCATC AAATCTTCAA 
CACCAGAAAA TCCGCATGGC CCTTGTAGAA CCACAGATAC CAGGAATAAA TATGCCTCCT 
GATGTTTCAG ACATGGATCT TAATGGACCA GGGGGAAAGA AGAGGAAACT TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An overview of pre-ribosomal RNA processing in eukaryotes.
Wiley interdisciplinary reviews. RNA6(2)225-42(2015 Mar-Apr)
Henras AK,Plisson-Chastang C,O'Donohue MF,Chakraborty A,Gleizes PE

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.
The EMBO journal29(14)2342-57(2010 Jul)
Tomecki R,Kristiansen MS,Lykke-Andersen S,Chlebowski A,Larsen KM,Szczesny RJ,Drazkowska K,Pastula A,Andersen JS,Stepien PP,Dziembowski A,Jensen TH

Dis3-like 1: a novel exoribonuclease associated with the human exosome.
The EMBO journal29(14)2358-67(2010 Jul)
Staals RH,Bronkhorst AW,Schilders G,Slomovic S,Schuster G,Heck AJ,Raijmakers R,Pruijn GJ

Addition of poly(A) and poly(A)-rich tails during RNA degradation in the cytoplasm of human cells.
Proceedings of the National Academy of Sciences of the United States of America107(16)7407-12(2010 Apr)
Slomovic S,Fremder E,Staals RH,Pruijn GJ,Schuster G

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Dis3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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