MAP3K1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K1
Entrez Gene ID	100913571
Full Name	mitogen-activated protein kinase kinase kinase 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003759374.3	XP_003759422.1	mitogen-activated protein kinase kinase kinase 1 isoform X2
XM_023495762.1	XP_023351530.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_031965235.1	XP_031821095.1	mitogen-activated protein kinase kinase kinase 1 isoform X1
XM_003759374.2	XP_003759422.1	mitogen-activated protein kinase kinase kinase 1
XM_003759374.4	XP_003759422.1	mitogen-activated protein kinase kinase kinase 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following MAP3K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa120979	XM_003759374.4 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa158311	XM_023495762.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa219732	XM_031965235.1 Latest version!	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OSa120979	XM_003759374.2	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSa120979	XM_003759374.3	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K1 ( XM_003759374.3 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003759422.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa120979
Clone ID Related Accession (Same CDS sequence)	XM_003759374.3 , XM_003759374.2 , XM_003759374.4
Accession Version	XM_003759374.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823171.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_003759374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003759422.1
CDS	699..4715
Translation	MENKETLKGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEINKLTPESQTEGHTNTSSPTPKGRRSPSPSSSSSSNRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLESSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRSTIQKFVSRMSNSHTLSSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSPSVLRAAQQQTVQQPPSVGSQRRNQDSNFNLTHYGVQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGNSGNNNGNTPSAGAASGSSQAGVSGDIVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQKLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQAGELAVGREILKSGSIGIGGIDYVLNCILGSQTESSNWQALLGRLCLIDRMLLEFPAEFYPHIVSGEVLQADAVVDRYKKLLSLLNFALQFIDNSHSMVGKLSRRVFLSAARMVARVPHVFLKLLEMLSVISSTHYTRMRRRLMAIADEMEIAEAIQLGMEDSLDCRQDGFLQPSAPDNSPETTENNSPQHTVQLEKTRKPLCDAKSSASPEEISDALAGISVGLPGSVTMEQPKPALQTKGRPHSQCLNSSSSSHPQLLFPTLPSPPSSAPSVPAGSVTDVSKPRTQGFVPCKIPTASPQMQRKFSLQILRNCSEIKEPDKLSPVFTQARPMPSSQIHRPKPSRPTPGDVHKQGEVSKSSMTLDLNNITQCDGGGGAVIPSEETVFTPVEEKGRLDVNTELNSSIEDLLEAMPASDTTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALSIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMTCAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_003759374.3
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003759374.3

ORF Insert Sequence:

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_003759374.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003759422.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.

Price & Availability↑

CloneID	OSa120979
Clone ID Related Accession (Same CDS sequence)	XM_003759374.3 , XM_003759374.2 , XM_003759374.4
Accession Version	XM_003759374.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823171.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003759374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003759422.1
CDS	98..4114
Translation	MENKETLKGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEINKLTPESQTEGHTNTSSPTPKGRRSPSPSSSSSSNRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLESSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRSTIQKFVSRMSNSHTLSSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSPSVLRAAQQQTVQQPPSVGSQRRNQDSNFNLTHYGVQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGNSGNNNGNTPSAGAASGSSQAGVSGDIVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQKLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQAGELAVGREILKSGSIGIGGIDYVLNCILGSQTESSNWQALLGRLCLIDRMLLEFPAEFYPHIVSGEVLQADAVVDRYKKLLSLLNFALQFIDNSHSMVGKLSRRVFLSAARMVARVPHVFLKLLEMLSVISSTHYTRMRRRLMAIADEMEIAEAIQLGMEDSLDCRQDGFLQPSAPDNSPETTENNSPQHTVQLEKTRKPLCDAKSSASPEEISDALAGISVGLPGSVTMEQPKPALQTKGRPHSQCLNSSSSSHPQLLFPTLPSPPSSAPSVPAGSVTDVSKPRTQGFVPCKIPTASPQMQRKFSLQILRNCSEIKEPDKLSPVFTQARPMPSSQIHRPKPSRPTPGDVHKQGEVSKSSMTLDLNNITQCDGGGGAVIPSEETVFTPVEEKGRLDVNTELNSSIEDLLEAMPASDTTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALSIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMTCAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_003759374.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase (MAP3K1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003759374.2

ORF Insert Sequence:

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_003759374.4 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003759422.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

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CloneID	OSa120979
Clone ID Related Accession (Same CDS sequence)	XM_003759374.3 , XM_003759374.2 , XM_003759374.4
Accession Version	XM_003759374.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_003759374.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003759422.1
CDS	693..4709
Translation	MENKETLKGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEINKLTPESQTEGHTNTSSPTPKGRRSPSPSSSSSSNRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLESSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRSTIQKFVSRMSNSHTLSSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSPSVLRAAQQQTVQQPPSVGSQRRNQDSNFNLTHYGVQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGNSGNNNGNTPSAGAASGSSQAGVSGDIVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQKLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQAGELAVGREILKSGSIGIGGIDYVLNCILGSQTESSNWQALLGRLCLIDRMLLEFPAEFYPHIVSGEVLQADAVVDRYKKLLSLLNFALQFIDNSHSMVGKLSRRVFLSAARMVARVPHVFLKLLEMLSVISSTHYTRMRRRLMAIADEMEIAEAIQLGMEDSLDCRQDGFLQPSAPDNSPETTENNSPQHTVQLEKTRKPLCDAKSSASPEEISDALAGISVGLPGSVTMEQPKPALQTKGRPHSQCLNSSSSSHPQLLFPTLPSPPSSAPSVPAGSVTDVSKPRTQGFVPCKIPTASPQMQRKFSLQILRNCSEIKEPDKLSPVFTQARPMPSSQIHRPKPSRPTPGDVHKQGEVSKSSMTLDLNNITQCDGGGGAVIPSEETVFTPVEEKGRLDVNTELNSSIEDLLEAMPASDTTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALSIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMTCAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_003759374.4
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003759374.4

ORF Insert Sequence:

ATGGAGAATA AAGAAACTCT CAAGGGATTG CAGAAAATGG ATGACCGCCC AGAAGAACGA 
ATGATCAGGG AAAAACTCAA GGCCACATGT ATGCCAGCCT GGAAACATGA ATGGCTGGAA 
AGGAGGAGTA GGAGAGGCCC TGTGGTAGTA AAACCAATTC CTGTCAAAGG AGATGGTTCT 
GAAATCAACA AGTTAACACC AGAATCTCAA ACTGAAGGGC ATACAAATAC TTCTTCACCT 
ACTCCAAAAG GAAGGCGAAG TCCTTCTCCA AGTAGCTCTT CTTCTTCAAA TCGTACTGTT 
AAATCAGAAT CTCCTGGGGT AAGAAGAAAA AGAGTATCTC CAGTGCCTTT TCAGAGTGGG 
AGAATCACAC CACCCCGAAG AGCTCCATCT CCAGATGGCT TCTCTCCATA TAGTCCTGAG 
GAAACAAATC GTCGAGTCAA CAAAGTCATG AGGGCTCGGT TGTACTTACT GCAGCAGATA 
GGACCCAACT CTTTCCTCAT TGGAGGAGAT AGCCCAGACA ATAAATACAG GGTGTTTATT 
GGGCCTCAGA CCTGTAGCTG TGGGCGTGGA ACATTTTGTA TCCATTTGTT ATTTGTTATG 
CTCCGAGTAT TTCAACTAGA ATCCTCAGAT CCAATGTTGT GGAGGAAAAC TTTGAAAAAT 
TTTGAGGTTG AAAGTTTGTT CCAGAAATAT CACAGTAGGC GTAGCTCAAG GATCAAAGCT 
CCATCTCGTA GCACCATCCA GAAGTTTGTA TCACGCATGT CAAACTCTCA TACGTTGTCA 
TCATCTAGTA CTTCTACATC TAGTTCAGAA AACAGTATGA AGGATGAAGA GGAACAGATG 
TGCCCCATTT GTCTGTTAGG AATGCTTGAT GAAGAGAGCC TTACAGTATG TGAAGATGGC 
TGTAGGAACA AGTTGCACCA CCACTGCATG TCAATTTGGG CAGAAGAATG TAGAAGAAAT 
AGAGAGCCTC TGATATGTCC TCTTTGTAGA TCCAAGTGGA GGTCCCATGA TTTCTACAGT 
CATGAATTGC CAAGTCCTGT GGATTCTCCT TCTGTTCTCC GAGCTGCCCA ACAACAAACT 
GTACAGCAGC CACCTTCTGT TGGATCACAA AGAAGAAACC AGGACAGTAA CTTTAACCTT 
ACTCATTATG GAGTTCAGCA GATCCCTCCT GCCTACAAAG ATTTAGCTGA GCCATGGATT 
CAGGTATTTG GAATGGAGCT TGTGGGCTGC TTATTTTCTC GAAACTGGAA TATAAGAGAG 
ATGGCACTCA GGCGTCTTTC CCATGATGTT AGTGGTGCTC TGCTATTGGC TAATGGGGAA 
AGCACTGGAA ATTCTGGGAA CAACAATGGA AACACCCCAA GTGCTGGAGC AGCAAGTGGG 
TCATCTCAGG CCGGTGTCTC TGGAGACATT GTTGTGGAGG CTTGCTGCAG TGTTCTGTCA 
ATGGTGTGTG CTGACCCTGT CTACAAAGTC TATGTTGCTG CTTTAAAAAC ATTAAGAGCC 
ATGTTGGTGT ACACTCCTTG CCACAGTTTG GCAGAAAGAA TAAAACTTCA GAAACTTCTT 
AGGCCAGTTG TAGAAACCAT ATTAGTCAAA TGTGCAGATG CCAATAGCCG CACAAGTCAG 
CTTTCAGTTT CAACACTGTT GGAACTCTGC AAAGGCCAAG CAGGAGAACT GGCAGTTGGC 
AGAGAAATAC TTAAATCAGG ATCCATTGGT ATTGGTGGTA TTGATTATGT TTTAAATTGT 
ATTCTTGGAA GCCAAACTGA ATCAAGTAAC TGGCAAGCAC TATTGGGGCG CCTTTGTCTT 
ATAGACAGAA TGTTGTTGGA GTTTCCTGCA GAATTTTATC CCCATATTGT TAGTGGAGAA 
GTCTTACAAG CTGATGCTGT AGTAGACAGA TATAAGAAGC TGCTGTCCCT CTTGAACTTT 
GCTTTGCAAT TCATTGATAA TTCCCACTCA ATGGTTGGTA AACTGTCCCG AAGAGTGTTT 
TTAAGTGCTG CAAGAATGGT TGCCAGAGTA CCCCATGTGT TTTTAAAACT CTTGGAAATG 
TTGAGTGTTA TAAGCTCTAC ACATTACACA AGGATGCGTC GTCGTTTGAT GGCTATTGCA 
GATGAGATGG AAATTGCTGA AGCCATCCAG CTGGGCATGG AAGATTCTTT GGATTGCAGG 
CAGGATGGCT TTTTGCAGCC ATCCGCCCCA GATAACTCCC CAGAAACAAC AGAAAACAAT 
TCTCCTCAGC ACACAGTCCA GTTAGAGAAA ACCAGAAAGC CTTTGTGTGA TGCAAAATCA 
AGTGCCAGTC CAGAAGAGAT CTCGGATGCC TTAGCTGGCA TTTCTGTGGG ACTTCCCGGT 
TCAGTAACAA TGGAGCAACC AAAGCCAGCC CTTCAGACGA AAGGTAGACC CCACAGTCAG 
TGTTTGAACT CTTCTTCATC CAGTCACCCC CAGTTATTGT TCCCAACTTT GCCCTCCCCT 
CCTTCCTCTG CCCCATCTGT ACCAGCTGGC TCTGTAACAG ATGTCTCTAA ACCCAGAACT 
CAGGGATTTG TTCCCTGCAA AATACCCACA GCTTCTCCAC AAATGCAAAG AAAATTTTCT 
CTACAGATTC TTAGAAACTG TTCTGAAATC AAAGAACCAG ATAAACTTTC CCCAGTTTTT 
ACGCAGGCAA GACCCATGCC TTCCAGTCAG ATACACAGGC CAAAGCCTTC TCGGCCCACT 
CCAGGAGATG TGCACAAGCA GGGAGAAGTC TCCAAAAGTA GCATGACCCT GGACCTGAAC 
AACATCACCC AGTGCGATGG CGGTGGTGGT GCCGTCATCC CCAGTGAAGA GACGGTGTTC 
ACCCCGGTGG AGGAGAAGGG CCGCTTAGAT GTCAACACGG AGCTCAACTC CAGCATCGAG 
GATCTGCTTG AGGCCATGCC CGCCAGTGAC ACCACCGTGA CCTTCAAGTC AGAAGTGGCT 
GTCCTTTCTC CCGAGAGGGC CGAAAATGAT GACACCTACA AAGATGATGT AAATCACAAT 
CAGAAGTGCA AAGAGAAGAT GGAAGCGGAA GAAGAGGAAG CGTTATCCAT CGCCATGGCA 
ATGTCTGCCT CCCAGGATGC CCTGCCAATA GTTCCTCAGC TACAAGTTGA AAATGGAGAG 
GATATAATCA TTATACAGCA AGATACGCCA GAGACTCTTC CAGGACACAC CAAAGCAAAG 
CACCATTATA GAGAAGATGC CGAGTGGCTC AAAGGTCAGC AGATAGGTCT CGGAGCATTT 
TCTTCTTGTT ATCAAGCTCA AGATGTAGGA ACTGGAACTT TAATGGCTGT GAAACAGGTG 
ACATATGTCA GGAATACATC TTCTGAGCAA GAGGAGGTAG TTGAAGCTCT GAGAGAAGAG 
ATCAGGATGA TGAGCCACCT GAATCATCCT AATATCATTC GAATGTTGGG TGCTACATGT 
GAGAAGAGCA ATTATAATCT TTTCATAGAA TGGATGGCAG GAGGATCTGT GGCTCATTTA 
TTGAGTAAAT ATGGAGCTTT CAAGGAGTCA GTAGTTATTA ACTATACTGA GCAATTGCTT 
CGTGGCCTTT CTTATCTCCA TGAGAATCAG ATCATTCACA GAGATGTCAA AGGTGCCAAT 
TTACTAATTG ACAGCACAGG TCAGAGATTG AGAATTGCTG ACTTTGGAGC TGCAGCTAGA 
TTGGCATCAA AAGGAACGGG CGCTGGAGAG TTTCAGGGAC AGTTGTTGGG AACAATTGCA 
TTTATGGCAC CTGAGGTACT AAGAGGTCAA CAGTATGGTA GGAGTTGTGA TGTATGGAGT 
GTTGGCTGTG CTATTATAGA AATGACTTGT GCTAAACCAC CTTGGAATGC AGAAAAACAC 
TCTAATCATC TTGCTTTGAT ATTTAAGATT GCTAGTGCAA CTACTGCTCC TTCAATACCC 
TCCCATCTAT CTCCTGGCTT ACGAGATGTG ACTCTTCGTT GTTTAGAACT TCAACCTCAG 
GACAGACCTC CTTCAAGAGA GCTACTGAAG CATCCAGTCT TCCGTACTAC CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_023495762.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351530.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa158311
Clone ID Related Accession (Same CDS sequence)	XM_023495762.1
Accession Version	XM_023495762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823171.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGCA TTTTTTTCTA TTTTAGTCGA GATATGGAGA ATAAAGAAAC TCTCAAGGGA 
TTGCAGAAAA TGGATGACCG CCCAGAAGAA CGAATGATCA GGGAAAAACT CAAGGCCACA 
TGTATGCCAG CCTGGAAACA TGAATGGCTG GAAAGGAGGA GTAGGAGAGG CCCTGTGGTA 
GTAAAACCAA TTCCTGTCAA AGGAGATGGT TCTGAAATCA ACAAGTTAAC ACCAGAATCT 
CAAACTGAAG GGCATACAAA TACTTCTTCA CCTACTCCAA AAGGAAGGCG AAGTCCTTCT 
CCAAGTAGCT CTTCTTCTTC AAATCGTACT GTTAAATCAG AATCTCCTGG GGTAAGAAGA 
AAAAGAGTAT CTCCAGTGCC TTTTCAGAGT GGGAGAATCA CACCACCCCG AAGAGCTCCA 
TCTCCAGATG GCTTCTCTCC ATATAGTCCT GAGGAAACAA ATCGTCGAGT CAACAAAGTC 
ATGAGGGCTC GGTTGTACTT ACTGCAGCAG ATAGGACCCA ACTCTTTCCT CATTGGAGGA 
GATAGCCCAG ACAATAAATA CAGGGTGTTT ATTGGGCCTC AGACCTGTAG CTGTGGGCGT 
GGAACATTTT GTATCCATTT GTTATTTGTT ATGCTCCGAG TATTTCAACT AGAATCCTCA 
GATCCAATGT TGTGGAGGAA AACTTTGAAA AATTTTGAGG TTGAAAGTTT GTTCCAGAAA 
TATCACAGTA GGCGTAGCTC AAGGATCAAA GCTCCATCTC GTAGCACCAT CCAGAAGTTT 
GTATCACGCA TGTCAAACTC TCATACGTTG TCATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAGGATGA AGAGGAACAG ATGTGCCCCA TTTGTCTGTT AGGAATGCTT 
GATGAAGAGA GCCTTACAGT ATGTGAAGAT GGCTGTAGGA ACAAGTTGCA CCACCACTGC 
ATGTCAATTT GGGCAGAAGA ATGTAGAAGA AATAGAGAGC CTCTGATATG TCCTCTTTGT 
AGATCCAAGT GGAGGTCCCA TGATTTCTAC AGTCATGAAT TGCCAAGTCC TGTGGATTCT 
CCTTCTGTTC TCCGAGCTGC CCAACAACAA ACTGTACAGC AGCCACCTTC TGTTGGATCA 
CAAAGAAGAA ACCAGGACAG TAACTTTAAC CTTACTCATT ATGGAGTTCA GCAGATCCCT 
CCTGCCTACA AAGATTTAGC TGAGCCATGG ATTCAGGTAT TTGGAATGGA GCTTGTGGGC 
TGCTTATTTT CTCGAAACTG GAATATAAGA GAGATGGCAC TCAGGCGTCT TTCCCATGAT 
GTTAGTGGTG CTCTGCTATT GGCTAATGGG GAAAGCACTG GAAATTCTGG GAACAACAAT 
GGAAACACCC CAAGTGCTGG AGCAGCAAGT GGGTCATCTC AGGCCGGTGT CTCTGGAGAC 
ATTGTTGTGG AGGCTTGCTG CAGTGTTCTG TCAATGGTGT GTGCTGACCC TGTCTACAAA 
GTCTATGTTG CTGCTTTAAA AACATTAAGA GCCATGTTGG TGTACACTCC TTGCCACAGT 
TTGGCAGAAA GAATAAAACT TCAGAAACTT CTTAGGCCAG TTGTAGAAAC CATATTAGTC 
AAATGTGCAG ATGCCAATAG CCGCACAAGT CAGCTTTCAG TTTCAACACT GTTGGAACTC 
TGCAAAGGCC AAGCAGGAGA ACTGGCAGTT GGCAGAGAAA TACTTAAATC AGGATCCATT 
GGTATTGGTG GTATTGATTA TGTTTTAAAT TGTATTCTTG GAAGCCAAAC TGAATCAAGT 
AACTGGCAAG CACTATTGGG GCGCCTTTGT CTTATAGACA GAATGTTGTT GGAGTTTCCT 
GCAGAATTTT ATCCCCATAT TGTTAGTGGA GAAGTCTTAC AAGCTGATGC TGTAGTAGAC 
AGATATAAGA AGCTGCTGTC CCTCTTGAAC TTTGCTTTGC AATTCATTGA TAATTCCCAC 
TCAATGGTTG GTAAACTGTC CCGAAGAGTG TTTTTAAGTG CTGCAAGAAT GGTTGCCAGA 
GTACCCCATG TGTTTTTAAA ACTCTTGGAA ATGTTGAGTG TTATAAGCTC TACACATTAC 
ACAAGGATGC GTCGTCGTTT GATGGCTATT GCAGATGAGA TGGAAATTGC TGAAGCCATC 
CAGCTGGGCA TGGAAGATTC TTTGGATTGC AGGCAGGATG GCTTTTTGCA GCCATCCGCC 
CCAGATAACT CCCCAGAAAC AACAGAAAAC AATTCTCCTC AGCACACAGT CCAGTTAGAG 
AAAACCAGAA AGCCTTTGTG TGATGCAAAA TCAAGTGCCA GTCCAGAAGA GATCTCGGAT 
GCCTTAGCTG GCATTTCTGT GGGACTTCCC GGTTCAGTAA CAATGGAGCA ACCAAAGCCA 
GCCCTTCAGA CGAAAGGTAG ACCCCACAGT CAGTGTTTGA ACTCTTCTTC ATCCAGTCAC 
CCCCAGTTAT TGTTCCCAAC TTTGCCCTCC CCTCCTTCCT CTGCCCCATC TGTACCAGCT 
GGCTCTGTAA CAGATGTCTC TAAACCCAGA ACTCAGGGAT TTGTTCCCTG CAAAATACCC 
ACAGCTTCTC CACAAATGCA AAGAAAATTT TCTCTACAGA TTCTTAGAAA CTGTTCTGAA 
ATCAAAGAAC CAGATAAACT TTCCCCAGTT TTTACGCAGG CAAGACCCAT GCCTTCCAGT 
CAGATACACA GGCCAAAGCC TTCTCGGCCC ACTCCAGGAG ATGTGCACAA GCAGGGAGAA 
GTCTCCAAAA GTAGCATGAC CCTGGACCTG AACAACATCA CCCAGTGCGA TGGCGGTGGT 
GGTGCCGTCA TCCCCAGTGA AGAGACGGTG TTCACCCCGG TGGAGGAGAA GGGCCGCTTA 
GATGTCAACA CGGAGCTCAA CTCCAGCATC GAGGATCTGC TTGAGGCCAT GCCCGCCAGT 
GACACCACCG TGACCTTCAA GTCAGAAGTG GCTGTCCTTT CTCCCGAGAG GGCCGAAAAT 
GATGACACCT ACAAAGATGA TGTAAATCAC AATCAGAAGT GCAAAGAGAA GATGGAAGCG 
GAAGAAGAGG AAGCGTTATC CATCGCCATG GCAATGTCTG CCTCCCAGGA TGCCCTGCCA 
ATAGTTCCTC AGCTACAAGT TGAAAATGGA GAGGATATAA TCATTATACA GCAAGATACG 
CCAGAGACTC TTCCAGGACA CACCAAAGCA AAGCACCATT ATAGAGAAGA TGCCGAGTGG 
CTCAAAGGTC AGCAGATAGG TCTCGGAGCA TTTTCTTCTT GTTATCAAGC TCAAGATGTA 
GGAACTGGAA CTTTAATGGC TGTGAAACAG GTGACATATG TCAGGAATAC ATCTTCTGAG 
CAAGAGGAGG TAGTTGAAGC TCTGAGAGAA GAGATCAGGA TGATGAGCCA CCTGAATCAT 
CCTAATATCA TTCGAATGTT GGGTGCTACA TGTGAGAAGA GCAATTATAA TCTTTTCATA 
GAATGGATGG CAGGAGGATC TGTGGCTCAT TTATTGAGTA AATATGGAGC TTTCAAGGAG 
TCAGTAGTTA TTAACTATAC TGAGCAATTG CTTCGTGGCC TTTCTTATCT CCATGAGAAT 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTACTAA TTGACAGCAC AGGTCAGAGA 
TTGAGAATTG CTGACTTTGG AGCTGCAGCT AGATTGGCAT CAAAAGGAAC GGGCGCTGGA 
GAGTTTCAGG GACAGTTGTT GGGAACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT 
CAACAGTATG GTAGGAGTTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGACT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CACTCTAATC ATCTTGCTTT GATATTTAAG 
ATTGCTAGTG CAACTACTGC TCCTTCAATA CCCTCCCATC TATCTCCTGG CTTACGAGAT 
GTGACTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCTTCAAG AGAGCTACTG 
AAGCATCCAG TCTTCCGTAC TACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351530.1
CDS	4123..8172
Translation	MKCIFFYFSRDMENKETLKGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEINKLTPESQTEGHTNTSSPTPKGRRSPSPSSSSSSNRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLESSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRSTIQKFVSRMSNSHTLSSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSPSVLRAAQQQTVQQPPSVGSQRRNQDSNFNLTHYGVQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGNSGNNNGNTPSAGAASGSSQAGVSGDIVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQKLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQAGELAVGREILKSGSIGIGGIDYVLNCILGSQTESSNWQALLGRLCLIDRMLLEFPAEFYPHIVSGEVLQADAVVDRYKKLLSLLNFALQFIDNSHSMVGKLSRRVFLSAARMVARVPHVFLKLLEMLSVISSTHYTRMRRRLMAIADEMEIAEAIQLGMEDSLDCRQDGFLQPSAPDNSPETTENNSPQHTVQLEKTRKPLCDAKSSASPEEISDALAGISVGLPGSVTMEQPKPALQTKGRPHSQCLNSSSSSHPQLLFPTLPSPPSSAPSVPAGSVTDVSKPRTQGFVPCKIPTASPQMQRKFSLQILRNCSEIKEPDKLSPVFTQARPMPSSQIHRPKPSRPTPGDVHKQGEVSKSSMTLDLNNITQCDGGGGAVIPSEETVFTPVEEKGRLDVNTELNSSIEDLLEAMPASDTTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALSIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMTCAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_023495762.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495762.1

ORF Insert Sequence:

ATGAAATGCA TTTTTTTCTA TTTTAGTCGA GATATGGAGA ATAAAGAAAC TCTCAAGGGA 
TTGCAGAAAA TGGATGACCG CCCAGAAGAA CGAATGATCA GGGAAAAACT CAAGGCCACA 
TGTATGCCAG CCTGGAAACA TGAATGGCTG GAAAGGAGGA GTAGGAGAGG CCCTGTGGTA 
GTAAAACCAA TTCCTGTCAA AGGAGATGGT TCTGAAATCA ACAAGTTAAC ACCAGAATCT 
CAAACTGAAG GGCATACAAA TACTTCTTCA CCTACTCCAA AAGGAAGGCG AAGTCCTTCT 
CCAAGTAGCT CTTCTTCTTC AAATCGTACT GTTAAATCAG AATCTCCTGG GGTAAGAAGA 
AAAAGAGTAT CTCCAGTGCC TTTTCAGAGT GGGAGAATCA CACCACCCCG AAGAGCTCCA 
TCTCCAGATG GCTTCTCTCC ATATAGTCCT GAGGAAACAA ATCGTCGAGT CAACAAAGTC 
ATGAGGGCTC GGTTGTACTT ACTGCAGCAG ATAGGACCCA ACTCTTTCCT CATTGGAGGA 
GATAGCCCAG ACAATAAATA CAGGGTGTTT ATTGGGCCTC AGACCTGTAG CTGTGGGCGT 
GGAACATTTT GTATCCATTT GTTATTTGTT ATGCTCCGAG TATTTCAACT AGAATCCTCA 
GATCCAATGT TGTGGAGGAA AACTTTGAAA AATTTTGAGG TTGAAAGTTT GTTCCAGAAA 
TATCACAGTA GGCGTAGCTC AAGGATCAAA GCTCCATCTC GTAGCACCAT CCAGAAGTTT 
GTATCACGCA TGTCAAACTC TCATACGTTG TCATCATCTA GTACTTCTAC ATCTAGTTCA 
GAAAACAGTA TGAAGGATGA AGAGGAACAG ATGTGCCCCA TTTGTCTGTT AGGAATGCTT 
GATGAAGAGA GCCTTACAGT ATGTGAAGAT GGCTGTAGGA ACAAGTTGCA CCACCACTGC 
ATGTCAATTT GGGCAGAAGA ATGTAGAAGA AATAGAGAGC CTCTGATATG TCCTCTTTGT 
AGATCCAAGT GGAGGTCCCA TGATTTCTAC AGTCATGAAT TGCCAAGTCC TGTGGATTCT 
CCTTCTGTTC TCCGAGCTGC CCAACAACAA ACTGTACAGC AGCCACCTTC TGTTGGATCA 
CAAAGAAGAA ACCAGGACAG TAACTTTAAC CTTACTCATT ATGGAGTTCA GCAGATCCCT 
CCTGCCTACA AAGATTTAGC TGAGCCATGG ATTCAGGTAT TTGGAATGGA GCTTGTGGGC 
TGCTTATTTT CTCGAAACTG GAATATAAGA GAGATGGCAC TCAGGCGTCT TTCCCATGAT 
GTTAGTGGTG CTCTGCTATT GGCTAATGGG GAAAGCACTG GAAATTCTGG GAACAACAAT 
GGAAACACCC CAAGTGCTGG AGCAGCAAGT GGGTCATCTC AGGCCGGTGT CTCTGGAGAC 
ATTGTTGTGG AGGCTTGCTG CAGTGTTCTG TCAATGGTGT GTGCTGACCC TGTCTACAAA 
GTCTATGTTG CTGCTTTAAA AACATTAAGA GCCATGTTGG TGTACACTCC TTGCCACAGT 
TTGGCAGAAA GAATAAAACT TCAGAAACTT CTTAGGCCAG TTGTAGAAAC CATATTAGTC 
AAATGTGCAG ATGCCAATAG CCGCACAAGT CAGCTTTCAG TTTCAACACT GTTGGAACTC 
TGCAAAGGCC AAGCAGGAGA ACTGGCAGTT GGCAGAGAAA TACTTAAATC AGGATCCATT 
GGTATTGGTG GTATTGATTA TGTTTTAAAT TGTATTCTTG GAAGCCAAAC TGAATCAAGT 
AACTGGCAAG CACTATTGGG GCGCCTTTGT CTTATAGACA GAATGTTGTT GGAGTTTCCT 
GCAGAATTTT ATCCCCATAT TGTTAGTGGA GAAGTCTTAC AAGCTGATGC TGTAGTAGAC 
AGATATAAGA AGCTGCTGTC CCTCTTGAAC TTTGCTTTGC AATTCATTGA TAATTCCCAC 
TCAATGGTTG GTAAACTGTC CCGAAGAGTG TTTTTAAGTG CTGCAAGAAT GGTTGCCAGA 
GTACCCCATG TGTTTTTAAA ACTCTTGGAA ATGTTGAGTG TTATAAGCTC TACACATTAC 
ACAAGGATGC GTCGTCGTTT GATGGCTATT GCAGATGAGA TGGAAATTGC TGAAGCCATC 
CAGCTGGGCA TGGAAGATTC TTTGGATTGC AGGCAGGATG GCTTTTTGCA GCCATCCGCC 
CCAGATAACT CCCCAGAAAC AACAGAAAAC AATTCTCCTC AGCACACAGT CCAGTTAGAG 
AAAACCAGAA AGCCTTTGTG TGATGCAAAA TCAAGTGCCA GTCCAGAAGA GATCTCGGAT 
GCCTTAGCTG GCATTTCTGT GGGACTTCCC GGTTCAGTAA CAATGGAGCA ACCAAAGCCA 
GCCCTTCAGA CGAAAGGTAG ACCCCACAGT CAGTGTTTGA ACTCTTCTTC ATCCAGTCAC 
CCCCAGTTAT TGTTCCCAAC TTTGCCCTCC CCTCCTTCCT CTGCCCCATC TGTACCAGCT 
GGCTCTGTAA CAGATGTCTC TAAACCCAGA ACTCAGGGAT TTGTTCCCTG CAAAATACCC 
ACAGCTTCTC CACAAATGCA AAGAAAATTT TCTCTACAGA TTCTTAGAAA CTGTTCTGAA 
ATCAAAGAAC CAGATAAACT TTCCCCAGTT TTTACGCAGG CAAGACCCAT GCCTTCCAGT 
CAGATACACA GGCCAAAGCC TTCTCGGCCC ACTCCAGGAG ATGTGCACAA GCAGGGAGAA 
GTCTCCAAAA GTAGCATGAC CCTGGACCTG AACAACATCA CCCAGTGCGA TGGCGGTGGT 
GGTGCCGTCA TCCCCAGTGA AGAGACGGTG TTCACCCCGG TGGAGGAGAA GGGCCGCTTA 
GATGTCAACA CGGAGCTCAA CTCCAGCATC GAGGATCTGC TTGAGGCCAT GCCCGCCAGT 
GACACCACCG TGACCTTCAA GTCAGAAGTG GCTGTCCTTT CTCCCGAGAG GGCCGAAAAT 
GATGACACCT ACAAAGATGA TGTAAATCAC AATCAGAAGT GCAAAGAGAA GATGGAAGCG 
GAAGAAGAGG AAGCGTTATC CATCGCCATG GCAATGTCTG CCTCCCAGGA TGCCCTGCCA 
ATAGTTCCTC AGCTACAAGT TGAAAATGGA GAGGATATAA TCATTATACA GCAAGATACG 
CCAGAGACTC TTCCAGGACA CACCAAAGCA AAGCACCATT ATAGAGAAGA TGCCGAGTGG 
CTCAAAGGTC AGCAGATAGG TCTCGGAGCA TTTTCTTCTT GTTATCAAGC TCAAGATGTA 
GGAACTGGAA CTTTAATGGC TGTGAAACAG GTGACATATG TCAGGAATAC ATCTTCTGAG 
CAAGAGGAGG TAGTTGAAGC TCTGAGAGAA GAGATCAGGA TGATGAGCCA CCTGAATCAT 
CCTAATATCA TTCGAATGTT GGGTGCTACA TGTGAGAAGA GCAATTATAA TCTTTTCATA 
GAATGGATGG CAGGAGGATC TGTGGCTCAT TTATTGAGTA AATATGGAGC TTTCAAGGAG 
TCAGTAGTTA TTAACTATAC TGAGCAATTG CTTCGTGGCC TTTCTTATCT CCATGAGAAT 
CAGATCATTC ACAGAGATGT CAAAGGTGCC AATTTACTAA TTGACAGCAC AGGTCAGAGA 
TTGAGAATTG CTGACTTTGG AGCTGCAGCT AGATTGGCAT CAAAAGGAAC GGGCGCTGGA 
GAGTTTCAGG GACAGTTGTT GGGAACAATT GCATTTATGG CACCTGAGGT ACTAAGAGGT 
CAACAGTATG GTAGGAGTTG TGATGTATGG AGTGTTGGCT GTGCTATTAT AGAAATGACT 
TGTGCTAAAC CACCTTGGAA TGCAGAAAAA CACTCTAATC ATCTTGCTTT GATATTTAAG 
ATTGCTAGTG CAACTACTGC TCCTTCAATA CCCTCCCATC TATCTCCTGG CTTACGAGAT 
GTGACTCTTC GTTGTTTAGA ACTTCAACCT CAGGACAGAC CTCCTTCAAG AGAGCTACTG 
AAGCATCCAG TCTTCCGTAC TACCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K1 ( XM_031965235.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031821095.1 Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa219732
Clone ID Related Accession (Same CDS sequence)	XM_031965235.1
Accession Version	XM_031965235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	mitogen-activated protein kinase kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGGAA TCGCACCTCG TCGTCAGGAT TACCCGGCTC CGCGGCGGCG 
GCGGCCAGCC CTGAAGCCGG CGGCGGGCTC CTGCGGGACG CGGGCAGCGG GGGCCGCGAG 
CGGACGGACT GGCGGCGGAG GCAGCTGCGC CAAGTGCGGA GCGTGGAGCT GGAGCCTCCG 
CTCTTCCCCG CCTCCCCGCC GCCCTCCTCG GCGTCCCCCT CCCCGGAGCC CGGGGACCCT 
CCGCTCCCGG GGCCGCCGGC CGCTGCGGGG GGCGGCTTCC AGCCCGCGGC GCCTCCGCCC 
CCGCCGCCGC CGCCCGCCGC TCCCCTCGCC CGCGGCGCCT GCCGGTCCCC CGAGCCGCCG 
CCGCCGGACA GCGGCGCACC GAGCCCGGCG GGAGCCGAGT CCGGAGACCG GAGGGCGGCC 
CCGAGCGAGC CGCCGCCCGC AGCTGCCGGT GCCCCCGCGA GTCGAGATAT GGAGAATAAA 
GAAACTCTCA AGGGATTGCA GAAAATGGAT GACCGCCCAG AAGAACGAAT GATCAGGGAA 
AAACTCAAGG CCACATGTAT GCCAGCCTGG AAACATGAAT GGCTGGAAAG GAGGAGTAGG 
AGAGGCCCTG TGGTAGTAAA ACCAATTCCT GTCAAAGGAG ATGGTTCTGA AATCAACAAG 
TTAACACCAG AATCTCAAAC TGAAGGGCAT ACAAATACTT CTTCACCTAC TCCAAAAGGA 
AGGCGAAGTC CTTCTCCAAG TAGCTCTTCT TCTTCAAATC GTACTGTTAA ATCAGAATCT 
CCTGGGGTAA GAAGAAAAAG AGTATCTCCA GTGCCTTTTC AGAGTGGGAG AATCACACCA 
CCCCGAAGAG CTCCATCTCC AGATGGCTTC TCTCCATATA GTCCTGAGGA AACAAATCGT 
CGAGTCAACA AAGTCATGAG GGCTCGGTTG TACTTACTGC AGCAGATAGG ACCCAACTCT 
TTCCTCATTG GAGGAGATAG CCCAGACAAT AAATACAGGG TGTTTATTGG GCCTCAGACC 
TGTAGCTGTG GGCGTGGAAC ATTTTGTATC CATTTGTTAT TTGTTATGCT CCGAGTATTT 
CAACTAGAAT CCTCAGATCC AATGTTGTGG AGGAAAACTT TGAAAAATTT TGAGGTTGAA 
AGTTTGTTCC AGAAATATCA CAGTAGGCGT AGCTCAAGGA TCAAAGCTCC ATCTCGTAGC 
ACCATCCAGA AGTTTGTATC ACGCATGTCA AACTCTCATA CGTTGTCATC ATCTAGTACT 
TCTACATCTA GTTCAGAAAA CAGTATGAAG GATGAAGAGG AACAGATGTG CCCCATTTGT 
CTGTTAGGAA TGCTTGATGA AGAGAGCCTT ACAGTATGTG AAGATGGCTG TAGGAACAAG 
TTGCACCACC ACTGCATGTC AATTTGGGCA GAAGAATGTA GAAGAAATAG AGAGCCTCTG 
ATATGTCCTC TTTGTAGATC CAAGTGGAGG TCCCATGATT TCTACAGTCA TGAATTGCCA 
AGTCCTGTGG ATTCTCCTTC TGTTCTCCGA GCTGCCCAAC AACAAACTGT ACAGCAGCCA 
CCTTCTGTTG GATCACAAAG AAGAAACCAG GACAGTAACT TTAACCTTAC TCATTATGGA 
GTTCAGCAGA TCCCTCCTGC CTACAAAGAT TTAGCTGAGC CATGGATTCA GGTATTTGGA 
ATGGAGCTTG TGGGCTGCTT ATTTTCTCGA AACTGGAATA TAAGAGAGAT GGCACTCAGG 
CGTCTTTCCC ATGATGTTAG TGGTGCTCTG CTATTGGCTA ATGGGGAAAG CACTGGAAAT 
TCTGGGAACA ACAATGGAAA CACCCCAAGT GCTGGAGCAG CAAGTGGGTC ATCTCAGGCC 
GGTGTCTCTG GAGACATTGT TGTGGAGGCT TGCTGCAGTG TTCTGTCAAT GGTGTGTGCT 
GACCCTGTCT ACAAAGTCTA TGTTGCTGCT TTAAAAACAT TAAGAGCCAT GTTGGTGTAC 
ACTCCTTGCC ACAGTTTGGC AGAAAGAATA AAACTTCAGA AACTTCTTAG GCCAGTTGTA 
GAAACCATAT TAGTCAAATG TGCAGATGCC AATAGCCGCA CAAGTCAGCT TTCAGTTTCA 
ACACTGTTGG AACTCTGCAA AGGCCAAGCA GGAGAACTGG CAGTTGGCAG AGAAATACTT 
AAATCAGGAT CCATTGGTAT TGGTGGTATT GATTATGTTT TAAATTGTAT TCTTGGAAGC 
CAAACTGAAT CAAGTAACTG GCAAGCACTA TTGGGGCGCC TTTGTCTTAT AGACAGAATG 
TTGTTGGAGT TTCCTGCAGA ATTTTATCCC CATATTGTTA GTGGAGAAGT CTTACAAGCT 
GATGCTGTAG TAGACAGATA TAAGAAGCTG CTGTCCCTCT TGAACTTTGC TTTGCAATTC 
ATTGATAATT CCCACTCAAT GGTTGGTAAA CTGTCCCGAA GAGTGTTTTT AAGTGCTGCA 
AGAATGGTTG CCAGAGTACC CCATGTGTTT TTAAAACTCT TGGAAATGTT GAGTGTTATA 
AGCTCTACAC ATTACACAAG GATGCGTCGT CGTTTGATGG CTATTGCAGA TGAGATGGAA 
ATTGCTGAAG CCATCCAGCT GGGCATGGAA GATTCTTTGG ATTGCAGGCA GGATGGCTTT 
TTGCAGCCAT CCGCCCCAGA TAACTCCCCA GAAACAACAG AAAACAATTC TCCTCAGCAC 
ACAGTCCAGT TAGAGAAAAC CAGAAAGCCT TTGTGTGATG CAAAATCAAG TGCCAGTCCA 
GAAGAGATCT CGGATGCCTT AGCTGGCATT TCTGTGGGAC TTCCCGGTTC AGTAACAATG 
GAGCAACCAA AGCCAGCCCT TCAGACGAAA GGTAGACCCC ACAGTCAGTG TTTGAACTCT 
TCTTCATCCA GTCACCCCCA GTTATTGTTC CCAACTTTGC CCTCCCCTCC TTCCTCTGCC 
CCATCTGTAC CAGCTGGCTC TGTAACAGAT GTCTCTAAAC CCAGAACTCA GGGATTTGTT 
CCCTGCAAAA TACCCACAGC TTCTCCACAA ATGCAAAGAA AATTTTCTCT ACAGATTCTT 
AGAAACTGTT CTGAAATCAA AGAACCAGAT AAACTTTCCC CAGTTTTTAC GCAGGCAAGA 
CCCATGCCTT CCAGTCAGAT ACACAGGCCA AAGCCTTCTC GGCCCACTCC AGGAGATGTG 
CACAAGCAGG GAGAAGTCTC CAAAAGTAGC ATGACCCTGG ACCTGAACAA CATCACCCAG 
TGCGATGGCG GTGGTGGTGC CGTCATCCCC AGTGAAGAGA CGGTGTTCAC CCCGGTGGAG 
GAGAAGGGCC GCTTAGATGT CAACACGGAG CTCAACTCCA GCATCGAGGA TCTGCTTGAG 
GCCATGCCCG CCAGTGACAC CACCGTGACC TTCAAGTCAG AAGTGGCTGT CCTTTCTCCC 
GAGAGGGCCG AAAATGATGA CACCTACAAA GATGATGTAA ATCACAATCA GAAGTGCAAA 
GAGAAGATGG AAGCGGAAGA AGAGGAAGCG TTATCCATCG CCATGGCAAT GTCTGCCTCC 
CAGGATGCCC TGCCAATAGT TCCTCAGCTA CAAGTTGAAA ATGGAGAGGA TATAATCATT 
ATACAGCAAG ATACGCCAGA GACTCTTCCA GGACACACCA AAGCAAAGCA CCATTATAGA 
GAAGATGCCG AGTGGCTCAA AGGTCAGCAG ATAGGTCTCG GAGCATTTTC TTCTTGTTAT 
CAAGCTCAAG ATGTAGGAAC TGGAACTTTA ATGGCTGTGA AACAGGTGAC ATATGTCAGG 
AATACATCTT CTGAGCAAGA GGAGGTAGTT GAAGCTCTGA GAGAAGAGAT CAGGATGATG 
AGCCACCTGA ATCATCCTAA TATCATTCGA ATGTTGGGTG CTACATGTGA GAAGAGCAAT 
TATAATCTTT TCATAGAATG GATGGCAGGA GGATCTGTGG CTCATTTATT GAGTAAATAT 
GGAGCTTTCA AGGAGTCAGT AGTTATTAAC TATACTGAGC AATTGCTTCG TGGCCTTTCT 
TATCTCCATG AGAATCAGAT CATTCACAGA GATGTCAAAG GTGCCAATTT ACTAATTGAC 
AGCACAGGTC AGAGATTGAG AATTGCTGAC TTTGGAGCTG CAGCTAGATT GGCATCAAAA 
GGAACGGGCG CTGGAGAGTT TCAGGGACAG TTGTTGGGAA CAATTGCATT TATGGCACCT 
GAGGTACTAA GAGGTCAACA GTATGGTAGG AGTTGTGATG TATGGAGTGT TGGCTGTGCT 
ATTATAGAAA TGACTTGTGC TAAACCACCT TGGAATGCAG AAAAACACTC TAATCATCTT 
GCTTTGATAT TTAAGATTGC TAGTGCAACT ACTGCTCCTT CAATACCCTC CCATCTATCT 
CCTGGCTTAC GAGATGTGAC TCTTCGTTGT TTAGAACTTC AACCTCAGGA CAGACCTCCT 
TCAAGAGAGC TACTGAAGCA TCCAGTCTTC CGTACTACCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031821095.1
CDS	58..4542
Translation	MAAAAGNRTSSSGLPGSAAAAASPEAGGGLLRDAGSGGRERTDWRRRQLRQVRSVELEPPLFPASPPPSSASPSPEPGDPPLPGPPAAAGGGFQPAAPPPPPPPPAAPLARGACRSPEPPPPDSGAPSPAGAESGDRRAAPSEPPPAAAGAPASRDMENKETLKGLQKMDDRPEERMIREKLKATCMPAWKHEWLERRSRRGPVVVKPIPVKGDGSEINKLTPESQTEGHTNTSSPTPKGRRSPSPSSSSSSNRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETNRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQTCSCGRGTFCIHLLFVMLRVFQLESSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRSTIQKFVSRMSNSHTLSSSSTSTSSSENSMKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELPSPVDSPSVLRAAQQQTVQQPPSVGSQRRNQDSNFNLTHYGVQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNIREMALRRLSHDVSGALLLANGESTGNSGNNNGNTPSAGAASGSSQAGVSGDIVVEACCSVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQKLLRPVVETILVKCADANSRTSQLSVSTLLELCKGQAGELAVGREILKSGSIGIGGIDYVLNCILGSQTESSNWQALLGRLCLIDRMLLEFPAEFYPHIVSGEVLQADAVVDRYKKLLSLLNFALQFIDNSHSMVGKLSRRVFLSAARMVARVPHVFLKLLEMLSVISSTHYTRMRRRLMAIADEMEIAEAIQLGMEDSLDCRQDGFLQPSAPDNSPETTENNSPQHTVQLEKTRKPLCDAKSSASPEEISDALAGISVGLPGSVTMEQPKPALQTKGRPHSQCLNSSSSSHPQLLFPTLPSPPSSAPSVPAGSVTDVSKPRTQGFVPCKIPTASPQMQRKFSLQILRNCSEIKEPDKLSPVFTQARPMPSSQIHRPKPSRPTPGDVHKQGEVSKSSMTLDLNNITQCDGGGGAVIPSEETVFTPVEEKGRLDVNTELNSSIEDLLEAMPASDTTVTFKSEVAVLSPERAENDDTYKDDVNHNQKCKEKMEAEEEEALSIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKHHYREDAEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMTCAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVTLRCLELQPQDRPPSRELLKHPVFRTTW

Target ORF information:

RefSeq Version	XM_031965235.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii mitogen-activated protein kinase kinase kinase 1 (MAP3K1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031965235.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGGAA TCGCACCTCG TCGTCAGGAT TACCCGGCTC CGCGGCGGCG 
GCGGCCAGCC CTGAAGCCGG CGGCGGGCTC CTGCGGGACG CGGGCAGCGG GGGCCGCGAG 
CGGACGGACT GGCGGCGGAG GCAGCTGCGC CAAGTGCGGA GCGTGGAGCT GGAGCCTCCG 
CTCTTCCCCG CCTCCCCGCC GCCCTCCTCG GCGTCCCCCT CCCCGGAGCC CGGGGACCCT 
CCGCTCCCGG GGCCGCCGGC CGCTGCGGGG GGCGGCTTCC AGCCCGCGGC GCCTCCGCCC 
CCGCCGCCGC CGCCCGCCGC TCCCCTCGCC CGCGGCGCCT GCCGGTCCCC CGAGCCGCCG 
CCGCCGGACA GCGGCGCACC GAGCCCGGCG GGAGCCGAGT CCGGAGACCG GAGGGCGGCC 
CCGAGCGAGC CGCCGCCCGC AGCTGCCGGT GCCCCCGCGA GTCGAGATAT GGAGAATAAA 
GAAACTCTCA AGGGATTGCA GAAAATGGAT GACCGCCCAG AAGAACGAAT GATCAGGGAA 
AAACTCAAGG CCACATGTAT GCCAGCCTGG AAACATGAAT GGCTGGAAAG GAGGAGTAGG 
AGAGGCCCTG TGGTAGTAAA ACCAATTCCT GTCAAAGGAG ATGGTTCTGA AATCAACAAG 
TTAACACCAG AATCTCAAAC TGAAGGGCAT ACAAATACTT CTTCACCTAC TCCAAAAGGA 
AGGCGAAGTC CTTCTCCAAG TAGCTCTTCT TCTTCAAATC GTACTGTTAA ATCAGAATCT 
CCTGGGGTAA GAAGAAAAAG AGTATCTCCA GTGCCTTTTC AGAGTGGGAG AATCACACCA 
CCCCGAAGAG CTCCATCTCC AGATGGCTTC TCTCCATATA GTCCTGAGGA AACAAATCGT 
CGAGTCAACA AAGTCATGAG GGCTCGGTTG TACTTACTGC AGCAGATAGG ACCCAACTCT 
TTCCTCATTG GAGGAGATAG CCCAGACAAT AAATACAGGG TGTTTATTGG GCCTCAGACC 
TGTAGCTGTG GGCGTGGAAC ATTTTGTATC CATTTGTTAT TTGTTATGCT CCGAGTATTT 
CAACTAGAAT CCTCAGATCC AATGTTGTGG AGGAAAACTT TGAAAAATTT TGAGGTTGAA 
AGTTTGTTCC AGAAATATCA CAGTAGGCGT AGCTCAAGGA TCAAAGCTCC ATCTCGTAGC 
ACCATCCAGA AGTTTGTATC ACGCATGTCA AACTCTCATA CGTTGTCATC ATCTAGTACT 
TCTACATCTA GTTCAGAAAA CAGTATGAAG GATGAAGAGG AACAGATGTG CCCCATTTGT 
CTGTTAGGAA TGCTTGATGA AGAGAGCCTT ACAGTATGTG AAGATGGCTG TAGGAACAAG 
TTGCACCACC ACTGCATGTC AATTTGGGCA GAAGAATGTA GAAGAAATAG AGAGCCTCTG 
ATATGTCCTC TTTGTAGATC CAAGTGGAGG TCCCATGATT TCTACAGTCA TGAATTGCCA 
AGTCCTGTGG ATTCTCCTTC TGTTCTCCGA GCTGCCCAAC AACAAACTGT ACAGCAGCCA 
CCTTCTGTTG GATCACAAAG AAGAAACCAG GACAGTAACT TTAACCTTAC TCATTATGGA 
GTTCAGCAGA TCCCTCCTGC CTACAAAGAT TTAGCTGAGC CATGGATTCA GGTATTTGGA 
ATGGAGCTTG TGGGCTGCTT ATTTTCTCGA AACTGGAATA TAAGAGAGAT GGCACTCAGG 
CGTCTTTCCC ATGATGTTAG TGGTGCTCTG CTATTGGCTA ATGGGGAAAG CACTGGAAAT 
TCTGGGAACA ACAATGGAAA CACCCCAAGT GCTGGAGCAG CAAGTGGGTC ATCTCAGGCC 
GGTGTCTCTG GAGACATTGT TGTGGAGGCT TGCTGCAGTG TTCTGTCAAT GGTGTGTGCT 
GACCCTGTCT ACAAAGTCTA TGTTGCTGCT TTAAAAACAT TAAGAGCCAT GTTGGTGTAC 
ACTCCTTGCC ACAGTTTGGC AGAAAGAATA AAACTTCAGA AACTTCTTAG GCCAGTTGTA 
GAAACCATAT TAGTCAAATG TGCAGATGCC AATAGCCGCA CAAGTCAGCT TTCAGTTTCA 
ACACTGTTGG AACTCTGCAA AGGCCAAGCA GGAGAACTGG CAGTTGGCAG AGAAATACTT 
AAATCAGGAT CCATTGGTAT TGGTGGTATT GATTATGTTT TAAATTGTAT TCTTGGAAGC 
CAAACTGAAT CAAGTAACTG GCAAGCACTA TTGGGGCGCC TTTGTCTTAT AGACAGAATG 
TTGTTGGAGT TTCCTGCAGA ATTTTATCCC CATATTGTTA GTGGAGAAGT CTTACAAGCT 
GATGCTGTAG TAGACAGATA TAAGAAGCTG CTGTCCCTCT TGAACTTTGC TTTGCAATTC 
ATTGATAATT CCCACTCAAT GGTTGGTAAA CTGTCCCGAA GAGTGTTTTT AAGTGCTGCA 
AGAATGGTTG CCAGAGTACC CCATGTGTTT TTAAAACTCT TGGAAATGTT GAGTGTTATA 
AGCTCTACAC ATTACACAAG GATGCGTCGT CGTTTGATGG CTATTGCAGA TGAGATGGAA 
ATTGCTGAAG CCATCCAGCT GGGCATGGAA GATTCTTTGG ATTGCAGGCA GGATGGCTTT 
TTGCAGCCAT CCGCCCCAGA TAACTCCCCA GAAACAACAG AAAACAATTC TCCTCAGCAC 
ACAGTCCAGT TAGAGAAAAC CAGAAAGCCT TTGTGTGATG CAAAATCAAG TGCCAGTCCA 
GAAGAGATCT CGGATGCCTT AGCTGGCATT TCTGTGGGAC TTCCCGGTTC AGTAACAATG 
GAGCAACCAA AGCCAGCCCT TCAGACGAAA GGTAGACCCC ACAGTCAGTG TTTGAACTCT 
TCTTCATCCA GTCACCCCCA GTTATTGTTC CCAACTTTGC CCTCCCCTCC TTCCTCTGCC 
CCATCTGTAC CAGCTGGCTC TGTAACAGAT GTCTCTAAAC CCAGAACTCA GGGATTTGTT 
CCCTGCAAAA TACCCACAGC TTCTCCACAA ATGCAAAGAA AATTTTCTCT ACAGATTCTT 
AGAAACTGTT CTGAAATCAA AGAACCAGAT AAACTTTCCC CAGTTTTTAC GCAGGCAAGA 
CCCATGCCTT CCAGTCAGAT ACACAGGCCA AAGCCTTCTC GGCCCACTCC AGGAGATGTG 
CACAAGCAGG GAGAAGTCTC CAAAAGTAGC ATGACCCTGG ACCTGAACAA CATCACCCAG 
TGCGATGGCG GTGGTGGTGC CGTCATCCCC AGTGAAGAGA CGGTGTTCAC CCCGGTGGAG 
GAGAAGGGCC GCTTAGATGT CAACACGGAG CTCAACTCCA GCATCGAGGA TCTGCTTGAG 
GCCATGCCCG CCAGTGACAC CACCGTGACC TTCAAGTCAG AAGTGGCTGT CCTTTCTCCC 
GAGAGGGCCG AAAATGATGA CACCTACAAA GATGATGTAA ATCACAATCA GAAGTGCAAA 
GAGAAGATGG AAGCGGAAGA AGAGGAAGCG TTATCCATCG CCATGGCAAT GTCTGCCTCC 
CAGGATGCCC TGCCAATAGT TCCTCAGCTA CAAGTTGAAA ATGGAGAGGA TATAATCATT 
ATACAGCAAG ATACGCCAGA GACTCTTCCA GGACACACCA AAGCAAAGCA CCATTATAGA 
GAAGATGCCG AGTGGCTCAA AGGTCAGCAG ATAGGTCTCG GAGCATTTTC TTCTTGTTAT 
CAAGCTCAAG ATGTAGGAAC TGGAACTTTA ATGGCTGTGA AACAGGTGAC ATATGTCAGG 
AATACATCTT CTGAGCAAGA GGAGGTAGTT GAAGCTCTGA GAGAAGAGAT CAGGATGATG 
AGCCACCTGA ATCATCCTAA TATCATTCGA ATGTTGGGTG CTACATGTGA GAAGAGCAAT 
TATAATCTTT TCATAGAATG GATGGCAGGA GGATCTGTGG CTCATTTATT GAGTAAATAT 
GGAGCTTTCA AGGAGTCAGT AGTTATTAAC TATACTGAGC AATTGCTTCG TGGCCTTTCT 
TATCTCCATG AGAATCAGAT CATTCACAGA GATGTCAAAG GTGCCAATTT ACTAATTGAC 
AGCACAGGTC AGAGATTGAG AATTGCTGAC TTTGGAGCTG CAGCTAGATT GGCATCAAAA 
GGAACGGGCG CTGGAGAGTT TCAGGGACAG TTGTTGGGAA CAATTGCATT TATGGCACCT 
GAGGTACTAA GAGGTCAACA GTATGGTAGG AGTTGTGATG TATGGAGTGT TGGCTGTGCT 
ATTATAGAAA TGACTTGTGC TAAACCACCT TGGAATGCAG AAAAACACTC TAATCATCTT 
GCTTTGATAT TTAAGATTGC TAGTGCAACT ACTGCTCCTT CAATACCCTC CCATCTATCT 
CCTGGCTTAC GAGATGTGAC TCTTCGTTGT TTAGAACTTC AACCTCAGGA CAGACCTCCT 
TCAAGAGAGC TACTGAAGCA TCCAGTCTTC CGTACTACCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MAP3K1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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