Smpd3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smpd3
Entrez Gene ID	94338
Full Name	sphingomyelin phosphodiesterase 3
Synonyms	cca1
General protein information	Preferred Names sphingomyelin phosphodiesterase 3 Names sphingomyelin phosphodiesterase 3 confluent 3Y1 cell-associated 1 confluent 3Y1 cell-associated protein 1 nSMase-2 nSMase2 neutral sphingomyelin phosphodiesterase 3 neutral sphingomyelinase 2 neutral sphingomyelinase II sphingomyelin phosphodiesterase 3, neutral membrane
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	mRNA accumulation occurs in a growth arrested confluent monolayer but not in subconfluent cells; may play a role in regulation of cell growth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255543.3	XP_006255605.1	sphingomyelin phosphodiesterase 3 isoform X1
XM_017601396.1	XP_017456885.1	sphingomyelin phosphodiesterase 3 isoform X1
XM_008772541.2	XP_008770763.1	sphingomyelin phosphodiesterase 3 isoform X1
NM_053605.1	NP_446057.1	sphingomyelin phosphodiesterase 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SMPD3	NP_001179292.1
Mus musculus (house mouse)	Smpd3	NP_067466.1
Danio rerio (zebrafish)	smpd3	NP_001116222.1
Xenopus tropicalis (tropical clawed frog)	smpd3	XP_002939922.1
Pan troglodytes (chimpanzee)	SMPD3	XP_001167790.1
Canis lupus familiaris (dog)	SMPD3	XP_546863.1
Gallus gallus (chicken)	SMPD3	XP_429344.2
Homo sapiens (human)	SMPD3	NP_061137.1
Macaca mulatta (Rhesus monkey)	SMPD3	XP_001091683.1
Rattus norvegicus (Norway rat)	Smpd3	NP_446057.1

Gene Ontology
Bibliography

Related articles in PubMed

Enriched Expression of Neutral Sphingomyelinase 2 in the Striatum is Essential for Regulation of Lipid Raft Content and Motor Coordination.
Tan LH, Tan AJ, Ng YY, Chua JJ, Chew WS, Muralidharan S, Torta F, Dutta B, Sze SK, Herr DR, Ong WY
Molecular neurobiology55(7)5741-5756(2018 Jul)

Activation of neutral sphingomyelinase 2 by starvation induces cell-protective autophagy via an increase in Golgi-localized ceramide.
Back MJ, Ha HC, Fu Z, Choi JM, Piao Y, Won JH, Jang JM, Shin IC, Kim DK
Cell death & disease9(6)670(2018 06)

Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions.
Dickens AM, Tovar-Y-Romo LB, Yoo SW, Trout AL, Bae M, Kanmogne M, Megra B, Williams DW, Witwer KW, Gacias M, Tabatadze N, Cole RN, Casaccia P, Berman JW, Anthony DC, Haughey NJ
Science signaling10(473)(2017 Apr)

Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development.
Li J, Manickam G, Ray S, Oh CD, Yasuda H, Moffatt P, Murshed M
Molecular and cellular biology36(17)2282-99(2016 09)

Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state.
Dotson PP 2nd, Karakashian AA, Nikolova-Karakashian MN
The Biochemical journal465(3)371-82(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Smpd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smpd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10047	NM_053605.1 Latest version!	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa38893	XM_008772541.2 Latest version!	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa38893	XM_006255543.3 Latest version!	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa38893	XM_017601396.1 Latest version!	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smpd3 ( NM_053605.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446057.1 Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), mRNA.

Price & Availability↑

CloneID	ORa10047
Clone ID Related Accession (Same CDS sequence)	NM_053605.1
Accession Version	NM_053605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575820800000
Organism	Rattus norvegicus(Norway rat)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB000215.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB000215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAC GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGCTGTGC CCTGACTGGA 
AGGCTGAGGT GGAAGAATTC AGTTTTATCA CCCAGCTGTC CGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446057.1
CDS	363..2270
Translation	MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYEKRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQTPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGCALTGRLRWKNSVLSPSCPA

Target ORF information:

RefSeq Version	NM_053605.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053605.1

ORF Insert Sequence:

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAC GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGCTGTGC CCTGACTGGA 
AGGCTGAGGT GGAAGAATTC AGTTTTATCA CCCAGCTGTC CGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smpd3 ( XM_006255543.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255605.1 Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38893
Clone ID Related Accession (Same CDS sequence)	XM_006255543.3 , XM_017601396.1 , XM_008772541.2
Accession Version	XM_006255543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sphingomyelin phosphodiesterase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255605.1
CDS	471..2438
Translation	MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYEKRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQMPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSAGEEEA

Target ORF information:

RefSeq Version	XM_006255543.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255543.3

ORF Insert Sequence:

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smpd3 ( XM_017601396.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456885.1 Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38893
Clone ID Related Accession (Same CDS sequence)	XM_006255543.3 , XM_017601396.1 , XM_008772541.2
Accession Version	XM_017601396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sphingomyelin phosphodiesterase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456885.1
CDS	2287..4254
Translation	MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYEKRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQMPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSAGEEEA

Target ORF information:

RefSeq Version	XM_017601396.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601396.1

ORF Insert Sequence:

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smpd3 ( XM_008772541.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770763.1 Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X1, mRNA.

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CloneID	ORa38893
Clone ID Related Accession (Same CDS sequence)	XM_006255543.3 , XM_017601396.1 , XM_008772541.2
Accession Version	XM_008772541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	sphingomyelin phosphodiesterase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770763.1
CDS	405..2372
Translation	MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYEKRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQMPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFITQLSGLTDHLPVAMRLMVSAGEEEA

Target ORF information:

RefSeq Version	XM_008772541.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingomyelin phosphodiesterase 3 (Smpd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772541.2

ORF Insert Sequence:

ATGGTTTTGT ACACGACCCC CTTTCCTAAC AGCTGTCTGT CTGCCCTCCA CGCTGTGTCC 
TGGGCCCTCA TCTTCCCATG TTACTGGCTG GTGGACCGGC TGGTCGCCTC CTTCATACCC 
ACCACCTATG AGAAGCGCCA GAGGGCAGAT GACCCTTGCT ACCTGCAGCT GTTCTGCACC 
GTACTCTTCA CGCCAGTCTA CCTGGCTCTG CTTGTGGCTG CGCTGCCTTT TGCCTTTCTT 
GGGTTCATCT TCTGGTCTCC ACTGCAGTCT GCCCGCCGGC CCTACTCCTA CTCCCGGCTA 
GAAGACAAGA GCCCAGCTGG TGGGGCAGCC CTGCTTAGTG AATGGAAGGG CACAGGGGCT 
GGCAAAAGCT TTTGCTTTGC CACAGCCAAT GTCTGCCTCC TCCCTGACTC GCTCGCAAGG 
CTCAATAATG TTTTCAACAC CCAAGCCCGG GCCAAAGAGA TTGGGCAGAG AATCCGCAAT 
GGGGCCGCCC GGCCTCAGAT CAAAATCTAC ATTGATTCCC CCACCAACAC CTCCATCAGT 
GCAGCCAGCT TCAGCAGCCT GGTGTCGCCG CAGGGCAGTG ATGGGGCCAG AGCTGTTCCT 
GGGAGCATTA AGAGGACAGC CTCTGTGGAA TACAAGGGAG ATGGTGGGCG TCACCCCAGC 
GATGAGGCTG CCAATGGCCC AGCCTCTGGG GAACAGGCTG ATGGAAGCCT TGAGGACAGC 
TGCATTGTGC GAATTGGTGG TGAGGAAGGA GGCCGGGCAC AGGAAGCTGA TGACCCTGCT 
CCTGGGAGCC AAGCCAGGAA TGGGGCTGGT GGGACCCCAA AGGGCCAGAT GCCCAACCAC 
AATCAGCGGG ACGGGGATTC TGGGAGCCTG GGCAGCCCCT CCGCCTCCAG GGAGTCCTTG 
GTGAAGGCAC GGGCTGGACA AGACAGTGGT GGCAGCGGAG AGCCAGGCAG TAACAGTAAG 
CTGCTGTATA AGACCTCTGT GGTGAAGAAG GCAGCAGCCC GCAGGAGGCG CCACCCTGAT 
GAAGCCTTCG ACCATGAGGT CTCAGCCTTC TTCCCAGCCA ACCTGGACTT CCTGTGCCTG 
CAGGAGGTGT TTGACAAGCG TGCAGCTGCC AAGTTGAAAG AGCAGCTACA TGGCTACTTC 
GAGTACATCC TGTATGATGT TGGGGTCTAT GGCTGCCATG GTTGCTGCAA TTTCAAATGT 
CTCAACAGCG GTCTCTTCTT TGCCAGCCGC TACCCTGTTA TGGACGTGGC CTATCACTGT 
TACCCCAATG GGTGCAGCTT CGATGCCCTG GCCTCTAAGG GAGCTCTGTT TCTCAAGGTG 
CAGGTGGGAA GCACACCTCA GGACCAAAGA ATTGTTGGGT ACATCGCCTG TACACACCTG 
CATGCCCCAC CAGAGGACAG TGCCATCCGG TGTGAGCAGC TGGACCTGCT TCAGGACTGG 
CTGGCTGATT TCCGAAAATC TACCTCCTCG ACCAGCACAG CCAACCCCGA GGAGCTGGTG 
GTGTTTGACG TCATCTGTGG AGATTTGAAC TTTGATAACT GCTCTTCTGA TGACAAGCTG 
GAGCAGCAGC ATTCACTGTT TACTCGCTAC AAGGACCCCT GCCGCCTGGG GCCTGGGGAG 
GAAAAGCCTT GGGCCATTGG CACCCTGCTG GACATAAACG GTCTCTATGA TGAGGATGTG 
TGTACCCCAG ACAATCTTCA AAAGGTCTTG GAGAGTGAGG AAGGCCGCAG GGAGTATCTG 
GCCTTTCCCA CCAGCAAGAG CCCAGGGGCT GGACAGAAGG GACGGAAAGA TCTGTTGAAG 
GGCAATGGCC GCCGCATCGA CTACATGCTG CACGCTGAAG AGGGGCTGTG CCCTGACTGG 
AAGGCTGAGG TGGAAGAATT CAGTTTTATC ACCCAGCTGT CCGGCCTGAC CGACCACCTC 
CCCGTGGCCA TGCGGCTGAT GGTGTCTGCA GGGGAAGAGG AGGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enriched Expression of Neutral Sphingomyelinase 2 in the Striatum is Essential for Regulation of Lipid Raft Content and Motor Coordination.
Molecular neurobiology55(7)5741-5756(2018 Jul)
Tan LH,Tan AJ,Ng YY,Chua JJ,Chew WS,Muralidharan S,Torta F,Dutta B,Sze SK,Herr DR,Ong WY

Activation of neutral sphingomyelinase 2 by starvation induces cell-protective autophagy via an increase in Golgi-localized ceramide.
Cell death & disease9(6)670(2018 06)
Back MJ,Ha HC,Fu Z,Choi JM,Piao Y,Won JH,Jang JM,Shin IC,Kim DK

Astrocyte-shed extracellular vesicles regulate the peripheral leukocyte response to inflammatory brain lesions.
Science signaling10(473)(2017 Apr)
Dickens AM,Tovar-Y-Romo LB,Yoo SW,Trout AL,Bae M,Kanmogne M,Megra B,Williams DW,Witwer KW,Gacias M,Tabatadze N,Cole RN,Casaccia P,Berman JW,Anthony DC,Haughey NJ

Smpd3 Expression in both Chondrocytes and Osteoblasts Is Required for Normal Endochondral Bone Development.
Molecular and cellular biology36(17)2282-99(2016 09)
Li J,Manickam G,Ray S,Oh CD,Yasuda H,Moffatt P,Murshed M

Neutral sphingomyelinase-2 is a redox sensitive enzyme: role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state.
The Biochemical journal465(3)371-82(2015 Feb)
Dotson PP 2nd,Karakashian AA,Nikolova-Karakashian MN

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Smpd3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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