SMPD3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMPD3
Entrez Gene ID	568381
Full Name	sphingomyelin phosphodiesterase 3, neutral
Synonyms	si:ch73-316n6.2
General protein information	Preferred Names sphingomyelin phosphodiesterase 3, neutral Names sphingomyelin phosphodiesterase 3 neutral smase2 neutral sphingomyelinase 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163072.4	XP_005163129.1	sphingomyelin phosphodiesterase 3 isoform X1
NM_001122750.1	NP_001116222.1	sphingomyelin phosphodiesterase 3
XM_005163072.3	XP_005163129.1	sphingomyelin phosphodiesterase 3 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SMPD3	NP_001179292.1
Mus musculus (house mouse)	Smpd3	NP_067466.1
Danio rerio (zebrafish)	smpd3	NP_001116222.1
Xenopus tropicalis (tropical clawed frog)	smpd3	XP_002939922.1
Pan troglodytes (chimpanzee)	SMPD3	XP_001167790.1
Canis lupus familiaris (dog)	SMPD3	XP_546863.1
Gallus gallus (chicken)	SMPD3	XP_429344.2
Homo sapiens (human)	SMPD3	NP_061137.1
Macaca mulatta (Rhesus monkey)	SMPD3	XP_001091683.1
Rattus norvegicus (Norway rat)	Smpd3	NP_446057.1

Gene Ontology
Bibliography

Related articles in PubMed

Chemokine C-C motif ligand 33 is a key regulator of teleost fish barbel development.
Zhou T, Li N, Jin Y, Zeng Q, Prabowo W, Liu Y, Tian C, Bao L, Liu S, Yuan Z, Fu Q, Gao S, Gao D, Dunham R, Shubin NH, Liu Z
Proceedings of the National Academy of Sciences of the United States of America115(22)E5018-E5027(2018 05)

Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity.
Xu B, Zhang Y, Du XF, Li J, Zi HX, Bu JW, Yan Y, Han H, Du JL
Cell research27(7)882-897(2017 Jul)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Critical determinants of mitochondria-associated neutral sphingomyelinase (MA-nSMase) for mitochondrial localization.
Rajagopalan V, Canals D, Luberto C, Snider J, Voelkel-Johnson C, Obeid LM, Hannun YA
Biochimica et biophysica acta1850(4)628-39(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMPD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMPD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa29215	NM_001122750.1 Latest version!	Danio rerio sphingomyelin phosphodiesterase 3 (smpd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa29215	XM_005163072.4 Latest version!	Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ODa29215	XM_005163072.3	Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMPD3 ( NM_001122750.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001116222.1 Danio rerio sphingomyelin phosphodiesterase 3 (smpd3), mRNA.

Price & Availability↑

CloneID	ODa29215
Clone ID Related Accession (Same CDS sequence)	XM_005163072.4 , NM_001122750.1 , XM_005163072.3
Accession Version	NM_001122750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562947200000
Organism	Danio rerio(zebrafish)
Product	sphingomyelin phosphodiesterase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB361067.1. On Mar 19, 2008 this sequence version replaced XM_691706.2. ##Evidence-Data-START## Transcript exon combination :: AB361067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116222.1
CDS	1..2055
Translation	MVLHTSPYPSAFLSFLSGLSWAFVFPCYWLLDRLLASCVATSLEKRRRSQDPCSFLALGVLISTPLYLVLLLASLPFAFIGFLLWAPLQSIRKPYIYSHQKPDKHGVEQGSAAGTGAALAEWRPQGRSFCFGSANVCLLPDSLARFNNLADTQRRAREVGKRIRNGASRPQIKIYIDSPTNTSISAVSFCSLSTQGFRRTSSLDHRPDPVVTTENETEALTECPIHTSGGTSSDCPIHSTGVQNSSECPLHPNEEEHAPDCAMHQSSDSECPVHSNIMQNSEDCPLHPSGVQISITSPDSEPAEAEKGNHQTGDGDTGSLDSRTASRESLVRFHAADGGISPNNTLSQHRTSVFKRPIGRKRRHGDDGFDHEISAFFPANLDFLCLQEVFDRRAADRLRRQLHRYFPFVLSDVGRYGWKGCCSRFKFLNSGLLLASRYPILDAHYECFPNGRSEDALAAKGVLFAKVQVGSSAQDQRVVGYITCTHLHAIEGDAAVRCEQLDMLLEWGAEFRRVTSCPADEEKVLEDQVAFDVILGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLGPGEDKPWALGTYLDTSGIYDEEVSSPESLQKVMENEEGRKEYLVFPTSKNHCPSQKGRKIPLKGNGRRIDYILYKEEGLHQDWKVDIEEFSFITQLAGLTDHLSVAARLVVSTGEEET

Target ORF information:

RefSeq Version	NM_001122750.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sphingomyelin phosphodiesterase 3 (smpd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122750.1

ORF Insert Sequence:

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMPD3 ( XM_005163072.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163129.1 Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29215
Clone ID Related Accession (Same CDS sequence)	XM_005163072.4 , NM_001122750.1 , XM_005163072.3
Accession Version	XM_005163072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	sphingomyelin phosphodiesterase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163072.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163129.1
CDS	439..2493
Translation	MVLHTSPYPSAFLSFLSGLSWAFVFPCYWLLDRLLASCVATSLEKRRRSQDPCSFLALGVLISTPLYLVLLLASLPFAFIGFLLWAPLQSIRKPYIYSHQKPDKHGVEQGSAAGTGAALAEWRPQGRSFCFGSANVCLLPDSLARFNNLADTQRRAREVGKRIRNGASRPQIKIYIDSPTNTSISAVSFCSLSTQGFRRTSSLDHRPDPVVTTENETEALTECPIHTSGGTSSDCPIHSTGVQNSSECPLHPNEEEHAPDCAMHQSSDSECPVHSNIMQNSEDCPLHPSGVQISITSPDSEPAEAEKGNHQTGDGDTGSLDSRTASRESLVRFHAADGGISPNNTLSQHRTSVFKRPIGRKRRHGDDGFDHEISAFFPANLDFLCLQEVFDRRAADRLRRQLHRYFPFVLSDVGRYGWKGCCSRFKFLNSGLLLASRYPILDAHYECFPNGRSEDALAAKGVLFAKVQVGSSAQDQRVVGYITCTHLHAIEGDAAVRCEQLDMLLEWGAEFRRVTSCPADEEKVLEDQVAFDVILGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLGPGEDKPWALGTYLDTSGIYDEEVSSPESLQKVMENEEGRKEYLVFPTSKNHCPSQKGRKIPLKGNGRRIDYILYKEEGLHQDWKVDIEEFSFITQLAGLTDHLSVAARLVVSTGEEET

Target ORF information:

RefSeq Version	XM_005163072.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163072.4

ORF Insert Sequence:

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMPD3 ( XM_005163072.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163129.1 Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29215
Clone ID Related Accession (Same CDS sequence)	XM_005163072.4 , NM_001122750.1 , XM_005163072.3
Accession Version	XM_005163072.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	sphingomyelin phosphodiesterase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163129.1
CDS	432..2486
Translation	MVLHTSPYPSAFLSFLSGLSWAFVFPCYWLLDRLLASCVATSLEKRRRSQDPCSFLALGVLISTPLYLVLLLASLPFAFIGFLLWAPLQSIRKPYIYSHQKPDKHGVEQGSAAGTGAALAEWRPQGRSFCFGSANVCLLPDSLARFNNLADTQRRAREVGKRIRNGASRPQIKIYIDSPTNTSISAVSFCSLSTQGFRRTSSLDHRPDPVVTTENETEALTECPIHTSGGTSSDCPIHSTGVQNSSECPLHPNEEEHAPDCAMHQSSDSECPVHSNIMQNSEDCPLHPSGVQISITSPDSEPAEAEKGNHQTGDGDTGSLDSRTASRESLVRFHAADGGISPNNTLSQHRTSVFKRPIGRKRRHGDDGFDHEISAFFPANLDFLCLQEVFDRRAADRLRRQLHRYFPFVLSDVGRYGWKGCCSRFKFLNSGLLLASRYPILDAHYECFPNGRSEDALAAKGVLFAKVQVGSSAQDQRVVGYITCTHLHAIEGDAAVRCEQLDMLLEWGAEFRRVTSCPADEEKVLEDQVAFDVILGDLNFDNCSSEDKLEQQHSLFTHYKDPCRLGPGEDKPWALGTYLDTSGIYDEEVSSPESLQKVMENEEGRKEYLVFPTSKNHCPSQKGRKIPLKGNGRRIDYILYKEEGLHQDWKVDIEEFSFITQLAGLTDHLSVAARLVVSTGEEET

Target ORF information:

RefSeq Version	XM_005163072.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sphingomyelin phosphodiesterase 3, neutral (smpd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163072.3

ORF Insert Sequence:

ATGGTCTTGC ACACCTCTCC CTACCCCAGT GCCTTCCTCA GCTTTCTGAG TGGTCTGTCT 
TGGGCCTTTG TTTTCCCTTG CTACTGGCTA TTGGATCGCT TATTGGCCTC TTGTGTAGCA 
ACATCACTAG AGAAACGCAG ACGCTCTCAG GATCCCTGCT CTTTCCTGGC ACTTGGGGTT 
CTGATCTCTA CACCACTTTA CCTGGTGCTG CTACTAGCAT CCTTACCCTT TGCCTTCATT 
GGTTTTCTCT TGTGGGCACC ACTGCAGAGC ATCAGGAAAC CTTATATCTA CTCTCACCAG 
AAACCAGACA AGCATGGTGT GGAGCAGGGC TCTGCGGCTG GAACTGGGGC AGCACTTGCT 
GAGTGGCGTC CACAAGGACG CAGCTTTTGC TTTGGCAGTG CCAACGTTTG CTTGCTGCCA 
GACTCACTTG CACGCTTTAA CAACCTAGCG GACACTCAAC GCCGGGCTAG GGAAGTGGGC 
AAGCGAATTC GGAACGGTGC CAGCAGACCC CAAATCAAGA TATACATCGA CTCGCCCACA 
AATACTTCAA TCAGTGCTGT ATCCTTCTGC AGCTTGTCTA CCCAGGGCTT CCGCCGGACC 
TCTTCTTTGG ACCATCGTCC CGATCCTGTA GTCACCACTG AAAATGAGAC TGAGGCCTTA 
ACCGAGTGTC CCATTCACAC CTCAGGAGGT ACAAGTTCAG ATTGCCCCAT CCACAGCACT 
GGAGTCCAGA ATTCCTCAGA GTGCCCTCTC CATCCTAATG AAGAAGAGCA TGCTCCTGAT 
TGTGCCATGC ACCAGTCTTC AGATTCAGAG TGTCCAGTAC ATAGCAACAT AATGCAGAAC 
TCAGAAGATT GTCCACTCCA TCCATCTGGA GTGCAAATCA GCATCACCTC TCCTGATTCT 
GAGCCGGCTG AGGCTGAAAA AGGAAACCAT CAAACCGGGG ACGGGGACAC AGGTAGTCTA 
GACAGCCGCA CAGCCTCGCG AGAATCCCTG GTTCGCTTCC ATGCAGCCGA TGGAGGGATA 
TCTCCCAACA ACACCCTTTC ACAACACCGC ACCTCAGTCT TCAAAAGACC AATAGGACGC 
AAACGTCGTC ATGGAGACGA TGGCTTTGAC CACGAGATAT CTGCCTTCTT CCCAGCCAAC 
CTTGACTTCC TCTGTCTTCA GGAAGTCTTT GACAGGCGGG CCGCCGATAG GCTCAGGAGG 
CAGTTGCACC GCTATTTTCC CTTTGTACTG AGCGATGTAG GACGCTACGG CTGGAAAGGC 
TGTTGCTCCA GATTCAAGTT CTTAAACAGC GGTCTCCTGT TGGCCAGTCG CTACCCTATA 
TTGGATGCTC ATTATGAATG CTTCCCCAAT GGGCGCAGCG AAGATGCTCT GGCTGCTAAA 
GGAGTACTTT TTGCCAAGGT GCAGGTGGGA AGCTCCGCTC AGGACCAGCG TGTTGTTGGA 
TACATCACCT GTACTCATCT GCATGCTATT GAAGGCGATG CGGCGGTGCG CTGCGAGCAG 
TTGGACATGT TGTTGGAGTG GGGCGCAGAG TTTCGAAGGG TCACCTCGTG TCCCGCAGAT 
GAGGAGAAGG TGCTGGAGGA TCAGGTGGCC TTCGATGTGA TCCTCGGAGA CCTCAACTTC 
GACAACTGTT CCTCAGAGGA TAAGTTGGAG CAGCAGCATT CGCTCTTCAC TCATTATAAA 
GACCCGTGTC GTCTGGGACC AGGAGAGGAC AAACCCTGGG CTCTAGGTAC ATATTTGGAC 
ACAAGTGGGA TCTATGATGA GGAAGTCAGT TCACCAGAGA GCCTACAAAA GGTGATGGAG 
AATGAAGAGG GCAGAAAAGA GTATTTGGTG TTCCCCACAA GCAAGAACCA TTGTCCCAGT 
CAGAAGGGGC GCAAAATACC CCTGAAGGGA AACGGCAGGA GAATCGATTA CATCCTTTAT 
AAAGAGGAAG GCCTTCACCA GGACTGGAAA GTGGATATAG AGGAGTTCAG CTTCATCACT 
CAACTGGCTG GATTGACTGA CCATCTCTCA GTGGCTGCAA GATTGGTTGT GTCAACTGGA 
GAAGAGGAGA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chemokine C-C motif ligand 33 is a key regulator of teleost fish barbel development.
Proceedings of the National Academy of Sciences of the United States of America115(22)E5018-E5027(2018 05)
Zhou T,Li N,Jin Y,Zeng Q,Prabowo W,Liu Y,Tian C,Bao L,Liu S,Yuan Z,Fu Q,Gao S,Gao D,Dunham R,Shubin NH,Liu Z

Neurons secrete miR-132-containing exosomes to regulate brain vascular integrity.
Cell research27(7)882-897(2017 Jul)
Xu B,Zhang Y,Du XF,Li J,Zi HX,Bu JW,Yan Y,Han H,Du JL

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Critical determinants of mitochondria-associated neutral sphingomyelinase (MA-nSMase) for mitochondrial localization.
Biochimica et biophysica acta1850(4)628-39(2015 Apr)
Rajagopalan V,Canals D,Luberto C,Snider J,Voelkel-Johnson C,Obeid LM,Hannun YA

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SMPD3 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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