Gak cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gak
Entrez Gene ID	81659
Full Name	cyclin G associated kinase
General protein information	Preferred Names cyclin G associated kinase Names cyclin-G-associated kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	may be a serine/threonine protein kinase; may be involved with nucleotide metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599389.1	XP_017454878.1	cyclin-G-associated kinase isoform X3
XM_006250584.3	XP_006250646.1	cyclin-G-associated kinase isoform X2
XM_006250583.3	XP_006250645.1	cyclin-G-associated kinase isoform X1
NM_031030.2	NP_112292.1	cyclin-G-associated kinase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
Macaca mulatta (Rhesus monkey)	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1

Gene Ontology
Bibliography

Related articles in PubMed

Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
Beilina A, Rudenko IN, Kaganovich A, Civiero L, Chau H, Kalia SK, Kalia LV, Lobbestael E, Chia R, Ndukwe K, Ding J, Nalls MA, , , Olszewski M, Hauser DN, Kumaran R, Lozano AM, Baekelandt V, Greene LE, Taymans JM, Greggio E, Cookson MR
Proceedings of the National Academy of Sciences of the United States of America111(7)2626-31(2014 Feb)

Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice.
Yim YI, Sun T, Wu LG, Raimondi A, De Camilli P, Eisenberg E, Greene LE
Proceedings of the National Academy of Sciences of the United States of America107(9)4412-7(2010 Mar)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Essential role of cyclin-G-associated kinase (Auxilin-2) in developing and mature mice.
Lee DW, Zhao X, Yim YI, Eisenberg E, Greene LE
Molecular biology of the cell19(7)2766-76(2008 Jul)

Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events.
Zhang CX, Engqvist-Goldstein AE, Carreno S, Owen DJ, Smythe E, Drubin DG
Traffic (Copenhagen, Denmark)6(12)1103-13(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gak gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gak cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07425	NM_031030.2 Latest version!	Rattus norvegicus cyclin G associated kinase (Gak), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa35813	XM_006250583.3 Latest version!	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa35814	XM_006250584.3 Latest version!	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa58430	XM_017599389.1 Latest version!	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gak ( NM_031030.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112292.1 Rattus norvegicus cyclin G associated kinase (Gak), mRNA.

Price & Availability↑

CloneID	ORa07425
Clone ID Related Accession (Same CDS sequence)	NM_031030.2
Accession Version	NM_031030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Rattus norvegicus(Norway rat)
Product	cyclin-G-associated kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D38560.1. On Aug 31, 2012 this sequence version replaced NM_031030.1. ##Evidence-Data-START## Transcript exon combination :: D38560.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTTGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAA CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCACC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGGGAAACCA GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCAGAG GCTGAGATCA CAGAGTCGGA CTCACCACAG 
AGCAGCAGCA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAC 
CCAGAGACTG GTGTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTAACCT GATTGCAGAT 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT GCCCTAGTGA AGAGAGAAAG 
CCTGGGGCTG GAGAAGATAC ACCAAGGCTG GCAGCTGGGA CCAGACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCTGAAGA AGGTGTCGAT 
CTCCTGGGGC TGCACTCTGA AGGGGACTTA AGGCCTGCTG CTCCCTTGCA GGCTAGCGGG 
GTCCAGTCTA GCAACACTGA CCTGTTGAGC TCCCTTCTTG AACCATCTGA TGCTTCTCAA 
GTGGGACCTC CTGGTGACCT GCTTGGTGGT GAGACTCCTC TGCTGTTAGC AAGCCCAGTT 
TCTCTTCTTG GGGTGCAGAG CAACCTGCAA GGAAAAGTCC CTGACACTGT GGACCCATTT 
GACCAGTTCC TGCTGCCATC CAGCTCGGAC ACCCAGCCCT GCTCCAAGCC TGATCTCTTT 
GGAGAGTTTC TCAACTCTGA CTCTGTAGCT TCCTCAACTG CCTTCCCATC GACCCACAGC 
GCCCCACCCC CATCCTGCAG CACTGCCTTC CTGCACCTGG GAGATCTGCC AGCAGAGCCC 
AACAAGGTGA TTGCTTCATC CAGCCACCCA GATCTGCTAG GAGGATGGGA TACGTGGGCT 
GAGACTGCTC TACCTGGGCC GGCCTCCATG CCAGTACCAG AAGGTACTCT CTTCTCTTCT 
GCAGGTCACC CAGCCCCTCC AGGCCCCAAC CCCAGCCAAA CCAAGTCTCA GAACCCAGAC 
CCATTTGCTG ACCTCAGTGA TCTCAGCTCT AGCCTCCAAG GCTTGCCTGC TGGACTTCCT 
GCAGGGAGCT TCGTTGGCAC ATCAGCCACC ACTCACAAAA GCAACAGCTC CTGGCAGACA 
ACTCGTCCCA CAGCCCCTGG AACCTCATGG CCCCCGCAGG CCAAGCCAGC CCCCAGAGCC 
TCTGAACAAC TAAGGTCTCA CTTTAGTGTG ATTGGGGCCC GAGAAGAGAG AGGTGTCCGT 
GCGCCCAGCT TTGCCCAAAA GCCAAAGGTC TCAGAAAATG ATTTTGAAGA TCTGCTGCCT 
AATCAAGGCT TCTCTAAGTC TGACAAGAAG GGGCCAAAGA CCATGGCAGA GATGCGGAAA 
CAGGAACTTG CCAGAGATAC AGACCCATTC AAATTGAAGC TTTTGGACTG GATTGAAGGC 
AAAGAGAGGA ATATTCGTGC ACTGCTGTCC ACTCTGCACA CAGTATTGTG GGATGGGGAG 
AGCCGCTGGA CACCTGTGAG TATGGCTGAC CTGGTGACTC CAGAGCAGGT GAAGAAGCAG 
TACCGCCGTG CAGTGCTGGT AGTGCATCCT GATAAGGCCA CAGGGCAGCC ATATGAACAG 
TCTGCCAAGA TGATCTTCAT GGAGCTGAAT GATGCATGGT CTGAGTTTGA AAACCAGGGC 
TCAAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112292.1
CDS	105..4022
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPSGGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGITVQGDVLTIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERKPGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLSSLLEPSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASMPVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQTTRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	NM_031030.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclin G associated kinase (Gak), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031030.2

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTTGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAA CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCACC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGGGAAACCA GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCAGAG GCTGAGATCA CAGAGTCGGA CTCACCACAG 
AGCAGCAGCA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAC 
CCAGAGACTG GTGTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTAACCT GATTGCAGAT 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT GCCCTAGTGA AGAGAGAAAG 
CCTGGGGCTG GAGAAGATAC ACCAAGGCTG GCAGCTGGGA CCAGACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCTGAAGA AGGTGTCGAT 
CTCCTGGGGC TGCACTCTGA AGGGGACTTA AGGCCTGCTG CTCCCTTGCA GGCTAGCGGG 
GTCCAGTCTA GCAACACTGA CCTGTTGAGC TCCCTTCTTG AACCATCTGA TGCTTCTCAA 
GTGGGACCTC CTGGTGACCT GCTTGGTGGT GAGACTCCTC TGCTGTTAGC AAGCCCAGTT 
TCTCTTCTTG GGGTGCAGAG CAACCTGCAA GGAAAAGTCC CTGACACTGT GGACCCATTT 
GACCAGTTCC TGCTGCCATC CAGCTCGGAC ACCCAGCCCT GCTCCAAGCC TGATCTCTTT 
GGAGAGTTTC TCAACTCTGA CTCTGTAGCT TCCTCAACTG CCTTCCCATC GACCCACAGC 
GCCCCACCCC CATCCTGCAG CACTGCCTTC CTGCACCTGG GAGATCTGCC AGCAGAGCCC 
AACAAGGTGA TTGCTTCATC CAGCCACCCA GATCTGCTAG GAGGATGGGA TACGTGGGCT 
GAGACTGCTC TACCTGGGCC GGCCTCCATG CCAGTACCAG AAGGTACTCT CTTCTCTTCT 
GCAGGTCACC CAGCCCCTCC AGGCCCCAAC CCCAGCCAAA CCAAGTCTCA GAACCCAGAC 
CCATTTGCTG ACCTCAGTGA TCTCAGCTCT AGCCTCCAAG GCTTGCCTGC TGGACTTCCT 
GCAGGGAGCT TCGTTGGCAC ATCAGCCACC ACTCACAAAA GCAACAGCTC CTGGCAGACA 
ACTCGTCCCA CAGCCCCTGG AACCTCATGG CCCCCGCAGG CCAAGCCAGC CCCCAGAGCC 
TCTGAACAAC TAAGGTCTCA CTTTAGTGTG ATTGGGGCCC GAGAAGAGAG AGGTGTCCGT 
GCGCCCAGCT TTGCCCAAAA GCCAAAGGTC TCAGAAAATG ATTTTGAAGA TCTGCTGCCT 
AATCAAGGCT TCTCTAAGTC TGACAAGAAG GGGCCAAAGA CCATGGCAGA GATGCGGAAA 
CAGGAACTTG CCAGAGATAC AGACCCATTC AAATTGAAGC TTTTGGACTG GATTGAAGGC 
AAAGAGAGGA ATATTCGTGC ACTGCTGTCC ACTCTGCACA CAGTATTGTG GGATGGGGAG 
AGCCGCTGGA CACCTGTGAG TATGGCTGAC CTGGTGACTC CAGAGCAGGT GAAGAAGCAG 
TACCGCCGTG CAGTGCTGGT AGTGCATCCT GATAAGGCCA CAGGGCAGCC ATATGAACAG 
TCTGCCAAGA TGATCTTCAT GGAGCTGAAT GATGCATGGT CTGAGTTTGA AAACCAGGGC 
TCAAGGCCCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006250583.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250645.1 Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35813
Clone ID Related Accession (Same CDS sequence)	XM_006250583.3
Accession Version	XM_006250583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAG CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCATC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGGGAAACCA GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCAGAG GCTGAGATCA CAGAGTCGGA CTCACCACAG 
AGCAGCAGCA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAC 
CCAGAGACTG GTGTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTAACCT GATTGCAGAT 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT GCCCTAGTGA AGAGAGAAAG 
CCTGGGGCTG GAGAAGATAC ACCAAGGCTG GCAGCTGGGA CCAGACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCTGAAGA AGGTGTCGAT 
CTCCTGGGGC TGCACTCTGA AGGGGACTTA AGGCCTGCTG CTCCCTTGCA GGCTAGCGGG 
GTCCAGTCTA GCAACACTGA CCTGTTGAGC TCCCTTCTTG AACCATCTGA TGCTTCTCAA 
GTGGGACCTC CTGGTGACCT GCTTGGTGTG GACCCATTTG ACCAGTTCCT GCTGCCATCC 
AGCTCGGACA CCCAGCCCTG CTCCAAGCCT GATCTCTTTG GAGAGTTTCT CAACTCTGAC 
TCTGTAGCTT CCTCAACTGC CTTCCCATCG ACCCACAGCG CCCCACCCCC ATCCTGCAGC 
ACTGCCTTCC TGCACCTGGG AGATCTGCCA GCAGAGCCCA ACAAGGTGAT TGCTTCATCC 
AGCCACCCAG ATCTGCTAGG AGGATGGGAT ACGTGGGCTG AGACTGCTCT ACCTGGGCCG 
GCCTCCATGC CAGTACCAGA AGGTACTCTC TTCTCTTCTG CAGGTCACCC AGCCCCTCCA 
GGCCCCAACC CCAGCCAAAC CAAGTCTCAG AACCCAGACC CATTTGCTGA CCTCAGTGAT 
CTCAGCTCTA GCCTCCAAGG CTTGCCTGCT GGACTTCCTG CAGGGAGCTT CGTTGGCACA 
TCAGCCACCA CTCACAAAAG CAACAGCTCC TGGCAGACAA CTCGTCCCAC AGCCCCTGGA 
ACCTCATGGC CCCCGCAGGC CAAGCCAGCC CCCAGAGCCT CTGAACAACT AAGGTCTCAC 
TTTAGTGTGA TTGGGGCCCG AGAAGAGAGA GGTGTCCGTG CGCCCAGCTT TGCCCAAAAG 
CCAAAGGTCT CAGAAAATGA TTTTGAAGAT CTGCTGCCTA ATCAAGGCTT CTCTAAGTCT 
GACAAGAAGG GGCCAAAGAC CATGGCAGAG ATGCGGAAAC AGGAACTTGC CAGAGATACA 
GACCCATTCA AATTGAAGCT TTTGGACTGG ATTGAAGGCA AAGAGAGGAA TATTCGTGCA 
CTGCTGTCCA CTCTGCACAC AGTATTGTGG GATGGGGAGA GCCGCTGGAC ACCTGTGAGT 
ATGGCTGACC TGGTGACTCC AGAGCAGGTG AAGAAGCAGT ACCGCCGTGC AGTGCTGGTA 
GTGCATCCTG ATAAGGCCAC AGGGCAGCCA TATGAACAGT CTGCCAAGAT GATCTTCATG 
GAGCTGAATG ATGCATGGTC TGAGTTTGAA AACCAGGGCT CAAGGCCCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250645.1
CDS	107..3943
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPSGGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERKPGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLSSLLEPSDASQVGPPGDLLGVDPFDQFLLPSSSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASMPVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQTTRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006250583.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250583.3

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAG CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCATC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGGGAAACCA GAGCTACCCC GGCAGCCGGG CTCCACAGCT 
CAGTATGATG CTGAGGCAGG GTCTCCAGAG GCTGAGATCA CAGAGTCGGA CTCACCACAG 
AGCAGCAGCA CGGACACCAA CCACTTTCTT CACACACTGG ATTGGCAGGA GGAAAAAGAC 
CCAGAGACTG GTGTAGACAA TACCTCTCCT AAGGAGAGTC AGTCTAACCT GATTGCAGAT 
GGAGATGGAA GTGAAGTATC AGATGAAGAA GAGGCTTCAT GCCCTAGTGA AGAGAGAAAG 
CCTGGGGCTG GAGAAGATAC ACCAAGGCTG GCAGCTGGGA CCAGACAGCA AGACTTAATA 
TTTGATGTGG GCATGCTGGC TGCCCCACAG GAGCCTGTAC AGCCTGAAGA AGGTGTCGAT 
CTCCTGGGGC TGCACTCTGA AGGGGACTTA AGGCCTGCTG CTCCCTTGCA GGCTAGCGGG 
GTCCAGTCTA GCAACACTGA CCTGTTGAGC TCCCTTCTTG AACCATCTGA TGCTTCTCAA 
GTGGGACCTC CTGGTGACCT GCTTGGTGTG GACCCATTTG ACCAGTTCCT GCTGCCATCC 
AGCTCGGACA CCCAGCCCTG CTCCAAGCCT GATCTCTTTG GAGAGTTTCT CAACTCTGAC 
TCTGTAGCTT CCTCAACTGC CTTCCCATCG ACCCACAGCG CCCCACCCCC ATCCTGCAGC 
ACTGCCTTCC TGCACCTGGG AGATCTGCCA GCAGAGCCCA ACAAGGTGAT TGCTTCATCC 
AGCCACCCAG ATCTGCTAGG AGGATGGGAT ACGTGGGCTG AGACTGCTCT ACCTGGGCCG 
GCCTCCATGC CAGTACCAGA AGGTACTCTC TTCTCTTCTG CAGGTCACCC AGCCCCTCCA 
GGCCCCAACC CCAGCCAAAC CAAGTCTCAG AACCCAGACC CATTTGCTGA CCTCAGTGAT 
CTCAGCTCTA GCCTCCAAGG CTTGCCTGCT GGACTTCCTG CAGGGAGCTT CGTTGGCACA 
TCAGCCACCA CTCACAAAAG CAACAGCTCC TGGCAGACAA CTCGTCCCAC AGCCCCTGGA 
ACCTCATGGC CCCCGCAGGC CAAGCCAGCC CCCAGAGCCT CTGAACAACT AAGGTCTCAC 
TTTAGTGTGA TTGGGGCCCG AGAAGAGAGA GGTGTCCGTG CGCCCAGCTT TGCCCAAAAG 
CCAAAGGTCT CAGAAAATGA TTTTGAAGAT CTGCTGCCTA ATCAAGGCTT CTCTAAGTCT 
GACAAGAAGG GGCCAAAGAC CATGGCAGAG ATGCGGAAAC AGGAACTTGC CAGAGATACA 
GACCCATTCA AATTGAAGCT TTTGGACTGG ATTGAAGGCA AAGAGAGGAA TATTCGTGCA 
CTGCTGTCCA CTCTGCACAC AGTATTGTGG GATGGGGAGA GCCGCTGGAC ACCTGTGAGT 
ATGGCTGACC TGGTGACTCC AGAGCAGGTG AAGAAGCAGT ACCGCCGTGC AGTGCTGGTA 
GTGCATCCTG ATAAGGCCAC AGGGCAGCCA TATGAACAGT CTGCCAAGAT GATCTTCATG 
GAGCTGAATG ATGCATGGTC TGAGTTTGAA AACCAGGGCT CAAGGCCCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_006250584.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250646.1 Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X2, mRNA.

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CloneID	ORa35814
Clone ID Related Accession (Same CDS sequence)	XM_006250584.3
Accession Version	XM_006250584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAG CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCATC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GACCCAGAGA CTGGTGTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTAA CCTGATTGCA GATGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATGCCCTAG TGAAGAGAGA AAGCCTGGGG CTGGAGAAGA TACACCAAGG 
CTGGCAGCTG GGACCAGACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCTGA AGAAGGTGTC GATCTCCTGG GGCTGCACTC TGAAGGGGAC 
TTAAGGCCTG CTGCTCCCTT GCAGGCTAGC GGGGTCCAGT CTAGCAACAC TGACCTGTTG 
AGCTCCCTTC TTGAACCATC TGATGCTTCT CAAGTGGGAC CTCCTGGTGA CCTGCTTGGT 
GGTGAGACTC CTCTGCTGTT AGCAAGCCCA GTTTCTCTTC TTGGGGTGCA GAGCAACCTG 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGCC ATCCAGCTCG 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCGACCCAC AGCGCCCCAC CCCCATCCTG CAGCACTGCC 
TTCCTGCACC TGGGAGATCT GCCAGCAGAG CCCAACAAGG TGATTGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGAGACTG CTCTACCTGG GCCGGCCTCC 
ATGCCAGTAC CAGAAGGTAC TCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCAGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCCA GACCCATTTG CTGACCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCTTGCC TGCTGGACTT CCTGCAGGGA GCTTCGTTGG CACATCAGCC 
ACCACTCACA AAAGCAACAG CTCCTGGCAG ACAACTCGTC CCACAGCCCC TGGAACCTCA 
TGGCCCCCGC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AACTAAGGTC TCACTTTAGT 
GTGATTGGGG CCCGAGAAGA GAGAGGTGTC CGTGCGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTGCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
TTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAAGAGA GGAATATTCG TGCACTGCTG 
TCCACTCTGC ACACAGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCATATGAA CAGTCTGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCAAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250646.1
CDS	107..3877
Translation	MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPSGGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERKPGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLSSLLEPSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASMPVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQTTRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_006250584.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250584.3

ORF Insert Sequence:

ATGTCGCTGC TGCAGTCTGC ACTGGACTTC CTGGCGGGCC CTGGTTCTCT TGGCGGAGCT 
GCCGGCCGTG ACCAGAGTGA CTTCGTGGGG CAGACTGTGG AGCTGGGCGA GCTGCGTCTG 
CGGGTGCGGC GGGTCCTGGC CGAGGGAGGG TTTGCATTTG TTTATGAAGC TCAAGATCTG 
GGAAGTGGCA GAGAGTATGC ATTAAAGAGA TTACTATCCA ATGAAGAGGA AAAGAACAGA 
GCCATCATTC AGGAAGTATG TTTCTTGAAA AAACTTTCTG GCCACCCCAA TATTGTCCAG 
TTCTGCTCTG CAGCATCCAT AGGAAAAGAG GAATCGGACA CTGGGCAGGC TGAGTTCCTC 
CTGCTTACGG AGCTCTGTAA AGGACAGCTG GTGGAGTTTC TCAGGAGAGT TGAATGTAAA 
GGCCCTCTAT CCTGCGACAG CATTCTGAAG ATCTTCTACC AGACATGCAG AGCAGTGCAG 
CACATGCACA GGCAGAAACC ACCCATCATC CACAGGGATC TCAAGGTTGA AAACTTACTG 
CTTAGTAACC AGGGGACCAT TAAGCTGTGT GACTTTGGCA GTGCCACAAC CATCTCCCAT 
TATCCTGACT ACAGCTGGAG CGCCCAGAAG CGAGCAATGG TGGAGGAAGA GATCACGAGG 
AACACCACAC CCATGTACAG AACGCCAGAA ATTGTAGACC TGTATTCTAA CTTCCCTATT 
GGCGAAAAGC AGGATATCTG GGCACTGGGC TGTATCTTAT ACCTGCTGTG TTTCCGGCAG 
CATCCTTTTG AAGATGGAGC AAAACTTCGG ATAGTCAATG GGAAGTATTC CATTCCTGTG 
AATGACACTC GTTACACAGT CTTCCATGAC CTTATTCGTG GCATGCTAAA GGTCAATCCA 
GAAGAGAGGC TATCCATTGC TGAAGTGGTC CGACAACTGC AGGAAATTGC AGCAGCCCGG 
AATGTGAACC CCAAAGCCCC CATCACAGAG CTTCTGGAGC AGAATGGTGG CTATGGGAAC 
TCAGGGCCTT CCCGAGCACA ACCACCTTCT GGGGGCCCTG TGAACAGCAG TGGAGTTTTG 
GCTCTGGCAG AGTATGACCA GCCCTATGGT GGGTTTCTCG ATATCCTACG GGGTGGGACA 
GAACGGCTCT TCACCAACCT CAAGGATACT TCCTCCAAGG TCATCCAGTC TGTGGCTAAC 
TATGCAAAGG GCGATCTTGA CATATCTTAC ATCACATCCA GGATTGCAGT GATGTCGTTC 
CCAGCAGAAG GTGTGGAGTC AGCAATCAAA AACAATATAG AGGATGTACG ATTGTTTCTG 
GATGCCAAGC ATCCAGGACA TTATGCTGTC TACAACCTTT CTCCAAGAAT ATACCGGGCT 
TCCAAGTTCC ACAATCGGGT CACTGAGTGT GGCTGGGCAG TCAGGCGGGC ACCACATCTC 
CACAGTTTGT ATACTCTATG CAGGAGCATG CATGCCTGGC TCCGGGAAGA CCACAGGAAC 
GTCTGTGTTG TACATTGCAT GGATGGGAGA GCTGCGTCTG CTGTGGCAGT CTGTGCATTC 
CTGTGCTTCT GCCGTCTCTT CAGCACTGCA GAGGCTGCTG TGTACATGTT CAGCATGAAG 
CGCTGCCCAC CAGGCATTTG GCCATCCCAC AAAAGGTACA TTGAATACGT GTGTGACATG 
GTGGCAGAGG AACCCATCAC GCCCCACAGC AAGCCAATGC TGGTGAAATC TGTTGTCATG 
ACCCCTGTGC CACTGTTCAG CAAGCAGAGG AATGGCTGCC GACCATTCTG TGAGGTCTAT 
GTTGGGGAGG AGCGTGTAAC CACCACATCC CAGGAGTATG ACAGGATGAA GGAATTTAAA 
ATTGAGGATG GCAAGGCTGT CATCCCCCTG GGCATAACAG TTCAAGGCGA TGTCCTCATC 
ATCATTTACC ATGCCAGATC CACTTTAGGA GGGAGACTGC AGGCCAAGAT GGCGTCCATG 
AAAATGTTCC AGATTCAGTT CCACACTGGG TTCGTGCCTC GAAACGCAAC CACTGTGAAA 
TTTGCAAAGT ATGACCTGGA TGCTTGTGAC ATTCAAGAGA AGTACCCAGA TCTGTTCCAG 
GTGAACCTGG AAGTGGAGGT GGAGCCTAGA GACAGGCCCA GCCGAGACGT TCCACCTTGG 
GAGAATACCA GCCTAAGGGG GTTAAACCCC AAGATCCTCT TTTCCAACAG GGAAGAGCAA 
CAGGACATTC TGTCTAAGTT TGAGGAAAAA GACCCAGAGA CTGGTGTAGA CAATACCTCT 
CCTAAGGAGA GTCAGTCTAA CCTGATTGCA GATGGAGATG GAAGTGAAGT ATCAGATGAA 
GAAGAGGCTT CATGCCCTAG TGAAGAGAGA AAGCCTGGGG CTGGAGAAGA TACACCAAGG 
CTGGCAGCTG GGACCAGACA GCAAGACTTA ATATTTGATG TGGGCATGCT GGCTGCCCCA 
CAGGAGCCTG TACAGCCTGA AGAAGGTGTC GATCTCCTGG GGCTGCACTC TGAAGGGGAC 
TTAAGGCCTG CTGCTCCCTT GCAGGCTAGC GGGGTCCAGT CTAGCAACAC TGACCTGTTG 
AGCTCCCTTC TTGAACCATC TGATGCTTCT CAAGTGGGAC CTCCTGGTGA CCTGCTTGGT 
GGTGAGACTC CTCTGCTGTT AGCAAGCCCA GTTTCTCTTC TTGGGGTGCA GAGCAACCTG 
CAAGGAAAAG TCCCTGACAC TGTGGACCCA TTTGACCAGT TCCTGCTGCC ATCCAGCTCG 
GACACCCAGC CCTGCTCCAA GCCTGATCTC TTTGGAGAGT TTCTCAACTC TGACTCTGTA 
GCTTCCTCAA CTGCCTTCCC ATCGACCCAC AGCGCCCCAC CCCCATCCTG CAGCACTGCC 
TTCCTGCACC TGGGAGATCT GCCAGCAGAG CCCAACAAGG TGATTGCTTC ATCCAGCCAC 
CCAGATCTGC TAGGAGGATG GGATACGTGG GCTGAGACTG CTCTACCTGG GCCGGCCTCC 
ATGCCAGTAC CAGAAGGTAC TCTCTTCTCT TCTGCAGGTC ACCCAGCCCC TCCAGGCCCC 
AACCCCAGCC AAACCAAGTC TCAGAACCCA GACCCATTTG CTGACCTCAG TGATCTCAGC 
TCTAGCCTCC AAGGCTTGCC TGCTGGACTT CCTGCAGGGA GCTTCGTTGG CACATCAGCC 
ACCACTCACA AAAGCAACAG CTCCTGGCAG ACAACTCGTC CCACAGCCCC TGGAACCTCA 
TGGCCCCCGC AGGCCAAGCC AGCCCCCAGA GCCTCTGAAC AACTAAGGTC TCACTTTAGT 
GTGATTGGGG CCCGAGAAGA GAGAGGTGTC CGTGCGCCCA GCTTTGCCCA AAAGCCAAAG 
GTCTCAGAAA ATGATTTTGA AGATCTGCTG CCTAATCAAG GCTTCTCTAA GTCTGACAAG 
AAGGGGCCAA AGACCATGGC AGAGATGCGG AAACAGGAAC TTGCCAGAGA TACAGACCCA 
TTCAAATTGA AGCTTTTGGA CTGGATTGAA GGCAAAGAGA GGAATATTCG TGCACTGCTG 
TCCACTCTGC ACACAGTATT GTGGGATGGG GAGAGCCGCT GGACACCTGT GAGTATGGCT 
GACCTGGTGA CTCCAGAGCA GGTGAAGAAG CAGTACCGCC GTGCAGTGCT GGTAGTGCAT 
CCTGATAAGG CCACAGGGCA GCCATATGAA CAGTCTGCCA AGATGATCTT CATGGAGCTG 
AATGATGCAT GGTCTGAGTT TGAAAACCAG GGCTCAAGGC CCCTCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gak ( XM_017599389.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454878.1 Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

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CloneID	ORa58430
Clone ID Related Accession (Same CDS sequence)	XM_017599389.1
Accession Version	XM_017599389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGGC AGAAACCACC CATCATCCAC AGGGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGCGC CCAGAAGCGA GCAATGGTGG AGGAAGAGAT CACGAGGAAC 
ACCACACCCA TGTACAGAAC GCCAGAAATT GTAGACCTGT ATTCTAACTT CCCTATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAAG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGGCA TGCTAAAGGT CAATCCAGAA 
GAGAGGCTAT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCCCGGAAT 
GTGAACCCCA AAGCCCCCAT CACAGAGCTT CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTCTGGG GGCCCTGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTATGGTGGG TTTCTCGATA TCCTACGGGG TGGGACAGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGCG ATCTTGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCGTTCCCA 
GCAGAAGGTG TGGAGTCAGC AATCAAAAAC AATATAGAGG ATGTACGATT GTTTCTGGAT 
GCCAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGAATATA CCGGGCTTCC 
AAGTTCCACA ATCGGGTCAC TGAGTGTGGC TGGGCAGTCA GGCGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGCAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTAC ATTGCATGGA TGGGAGAGCT GCGTCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATACGTGTG TGACATGGTG 
GCAGAGGAAC CCATCACGCC CCACAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCTGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CATTCTGTGA GGTCTATGTT 
GGGGAGGAGC GTGTAACCAC CACATCCCAG GAGTATGACA GGATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC ATAACAGTTC AAGGCGATGT CCTCATCATC 
ATTTACCATG CCAGATCCAC TTTAGGAGGG AGACTGCAGG CCAAGATGGC GTCCATGAAA 
ATGTTCCAGA TTCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAGTATG ACCTGGATGC TTGTGACATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTGGA GCCTAGAGAC AGGCCCAGCC GAGACGTTCC ACCTTGGGAG 
AATACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAACAG 
GACATTCTGT CTAAGTTTGG GAAACCAGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCAGAGGCT GAGATCACAG AGTCGGACTC ACCACAGAGC 
AGCAGCACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGACCCA 
GAGACTGGTG TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTAACCTGAT TGCAGATGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATGCC CTAGTGAAGA GAGAAAGCCT 
GGGGCTGGAG AAGATACACC AAGGCTGGCA GCTGGGACCA GACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CTGAAGAAGG TGTCGATCTC 
CTGGGGCTGC ACTCTGAAGG GGACTTAAGG CCTGCTGCTC CCTTGCAGGC TAGCGGGGTC 
CAGTCTAGCA ACACTGACCT GTTGAGCTCC CTTCTTGAAC CATCTGATGC TTCTCAAGTG 
GGACCTCCTG GTGACCTGCT TGGTGGTGAG ACTCCTCTGC TGTTAGCAAG CCCAGTTTCT 
CTTCTTGGGG TGCAGAGCAA CCTGCAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGCCATCCAG CTCGGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCGAC CCACAGCGCC 
CCACCCCCAT CCTGCAGCAC TGCCTTCCTG CACCTGGGAG ATCTGCCAGC AGAGCCCAAC 
AAGGTGATTG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAG 
ACTGCTCTAC CTGGGCCGGC CTCCATGCCA GTACCAGAAG GTACTCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCAGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCCAGACCCA 
TTTGCTGACC TCAGTGATCT CAGCTCTAGC CTCCAAGGCT TGCCTGCTGG ACTTCCTGCA 
GGGAGCTTCG TTGGCACATC AGCCACCACT CACAAAAGCA ACAGCTCCTG GCAGACAACT 
CGTCCCACAG CCCCTGGAAC CTCATGGCCC CCGCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAACTAA GGTCTCACTT TAGTGTGATT GGGGCCCGAG AAGAGAGAGG TGTCCGTGCG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT GCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCATTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAA 
GAGAGGAATA TTCGTGCACT GCTGTCCACT CTGCACACAG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCATA TGAACAGTCT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCA 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454878.1
CDS	185..3619
Translation	MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPSGGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEEKDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERKPGAGEDTPRLAAGTRQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLSSLLEPSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHPDLLGGWDTWAETALPGPASMPVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDLSSSLQGLPAGLPAGSFVGTSATTHKSNSSWQTTRPTAPGTSWPPQAKPAPRASEQLRSHFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF

Target ORF information:

RefSeq Version	XM_017599389.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclin G associated kinase (Gak), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599389.1

ORF Insert Sequence:

ATGCACAGGC AGAAACCACC CATCATCCAC AGGGATCTCA AGGTTGAAAA CTTACTGCTT 
AGTAACCAGG GGACCATTAA GCTGTGTGAC TTTGGCAGTG CCACAACCAT CTCCCATTAT 
CCTGACTACA GCTGGAGCGC CCAGAAGCGA GCAATGGTGG AGGAAGAGAT CACGAGGAAC 
ACCACACCCA TGTACAGAAC GCCAGAAATT GTAGACCTGT ATTCTAACTT CCCTATTGGC 
GAAAAGCAGG ATATCTGGGC ACTGGGCTGT ATCTTATACC TGCTGTGTTT CCGGCAGCAT 
CCTTTTGAAG ATGGAGCAAA ACTTCGGATA GTCAATGGGA AGTATTCCAT TCCTGTGAAT 
GACACTCGTT ACACAGTCTT CCATGACCTT ATTCGTGGCA TGCTAAAGGT CAATCCAGAA 
GAGAGGCTAT CCATTGCTGA AGTGGTCCGA CAACTGCAGG AAATTGCAGC AGCCCGGAAT 
GTGAACCCCA AAGCCCCCAT CACAGAGCTT CTGGAGCAGA ATGGTGGCTA TGGGAACTCA 
GGGCCTTCCC GAGCACAACC ACCTTCTGGG GGCCCTGTGA ACAGCAGTGG AGTTTTGGCT 
CTGGCAGAGT ATGACCAGCC CTATGGTGGG TTTCTCGATA TCCTACGGGG TGGGACAGAA 
CGGCTCTTCA CCAACCTCAA GGATACTTCC TCCAAGGTCA TCCAGTCTGT GGCTAACTAT 
GCAAAGGGCG ATCTTGACAT ATCTTACATC ACATCCAGGA TTGCAGTGAT GTCGTTCCCA 
GCAGAAGGTG TGGAGTCAGC AATCAAAAAC AATATAGAGG ATGTACGATT GTTTCTGGAT 
GCCAAGCATC CAGGACATTA TGCTGTCTAC AACCTTTCTC CAAGAATATA CCGGGCTTCC 
AAGTTCCACA ATCGGGTCAC TGAGTGTGGC TGGGCAGTCA GGCGGGCACC ACATCTCCAC 
AGTTTGTATA CTCTATGCAG GAGCATGCAT GCCTGGCTCC GGGAAGACCA CAGGAACGTC 
TGTGTTGTAC ATTGCATGGA TGGGAGAGCT GCGTCTGCTG TGGCAGTCTG TGCATTCCTG 
TGCTTCTGCC GTCTCTTCAG CACTGCAGAG GCTGCTGTGT ACATGTTCAG CATGAAGCGC 
TGCCCACCAG GCATTTGGCC ATCCCACAAA AGGTACATTG AATACGTGTG TGACATGGTG 
GCAGAGGAAC CCATCACGCC CCACAGCAAG CCAATGCTGG TGAAATCTGT TGTCATGACC 
CCTGTGCCAC TGTTCAGCAA GCAGAGGAAT GGCTGCCGAC CATTCTGTGA GGTCTATGTT 
GGGGAGGAGC GTGTAACCAC CACATCCCAG GAGTATGACA GGATGAAGGA ATTTAAAATT 
GAGGATGGCA AGGCTGTCAT CCCCCTGGGC ATAACAGTTC AAGGCGATGT CCTCATCATC 
ATTTACCATG CCAGATCCAC TTTAGGAGGG AGACTGCAGG CCAAGATGGC GTCCATGAAA 
ATGTTCCAGA TTCAGTTCCA CACTGGGTTC GTGCCTCGAA ACGCAACCAC TGTGAAATTT 
GCAAAGTATG ACCTGGATGC TTGTGACATT CAAGAGAAGT ACCCAGATCT GTTCCAGGTG 
AACCTGGAAG TGGAGGTGGA GCCTAGAGAC AGGCCCAGCC GAGACGTTCC ACCTTGGGAG 
AATACCAGCC TAAGGGGGTT AAACCCCAAG ATCCTCTTTT CCAACAGGGA AGAGCAACAG 
GACATTCTGT CTAAGTTTGG GAAACCAGAG CTACCCCGGC AGCCGGGCTC CACAGCTCAG 
TATGATGCTG AGGCAGGGTC TCCAGAGGCT GAGATCACAG AGTCGGACTC ACCACAGAGC 
AGCAGCACGG ACACCAACCA CTTTCTTCAC ACACTGGATT GGCAGGAGGA AAAAGACCCA 
GAGACTGGTG TAGACAATAC CTCTCCTAAG GAGAGTCAGT CTAACCTGAT TGCAGATGGA 
GATGGAAGTG AAGTATCAGA TGAAGAAGAG GCTTCATGCC CTAGTGAAGA GAGAAAGCCT 
GGGGCTGGAG AAGATACACC AAGGCTGGCA GCTGGGACCA GACAGCAAGA CTTAATATTT 
GATGTGGGCA TGCTGGCTGC CCCACAGGAG CCTGTACAGC CTGAAGAAGG TGTCGATCTC 
CTGGGGCTGC ACTCTGAAGG GGACTTAAGG CCTGCTGCTC CCTTGCAGGC TAGCGGGGTC 
CAGTCTAGCA ACACTGACCT GTTGAGCTCC CTTCTTGAAC CATCTGATGC TTCTCAAGTG 
GGACCTCCTG GTGACCTGCT TGGTGGTGAG ACTCCTCTGC TGTTAGCAAG CCCAGTTTCT 
CTTCTTGGGG TGCAGAGCAA CCTGCAAGGA AAAGTCCCTG ACACTGTGGA CCCATTTGAC 
CAGTTCCTGC TGCCATCCAG CTCGGACACC CAGCCCTGCT CCAAGCCTGA TCTCTTTGGA 
GAGTTTCTCA ACTCTGACTC TGTAGCTTCC TCAACTGCCT TCCCATCGAC CCACAGCGCC 
CCACCCCCAT CCTGCAGCAC TGCCTTCCTG CACCTGGGAG ATCTGCCAGC AGAGCCCAAC 
AAGGTGATTG CTTCATCCAG CCACCCAGAT CTGCTAGGAG GATGGGATAC GTGGGCTGAG 
ACTGCTCTAC CTGGGCCGGC CTCCATGCCA GTACCAGAAG GTACTCTCTT CTCTTCTGCA 
GGTCACCCAG CCCCTCCAGG CCCCAACCCC AGCCAAACCA AGTCTCAGAA CCCAGACCCA 
TTTGCTGACC TCAGTGATCT CAGCTCTAGC CTCCAAGGCT TGCCTGCTGG ACTTCCTGCA 
GGGAGCTTCG TTGGCACATC AGCCACCACT CACAAAAGCA ACAGCTCCTG GCAGACAACT 
CGTCCCACAG CCCCTGGAAC CTCATGGCCC CCGCAGGCCA AGCCAGCCCC CAGAGCCTCT 
GAACAACTAA GGTCTCACTT TAGTGTGATT GGGGCCCGAG AAGAGAGAGG TGTCCGTGCG 
CCCAGCTTTG CCCAAAAGCC AAAGGTCTCA GAAAATGATT TTGAAGATCT GCTGCCTAAT 
CAAGGCTTCT CTAAGTCTGA CAAGAAGGGG CCAAAGACCA TGGCAGAGAT GCGGAAACAG 
GAACTTGCCA GAGATACAGA CCCATTCAAA TTGAAGCTTT TGGACTGGAT TGAAGGCAAA 
GAGAGGAATA TTCGTGCACT GCTGTCCACT CTGCACACAG TATTGTGGGA TGGGGAGAGC 
CGCTGGACAC CTGTGAGTAT GGCTGACCTG GTGACTCCAG AGCAGGTGAA GAAGCAGTAC 
CGCCGTGCAG TGCTGGTAGT GCATCCTGAT AAGGCCACAG GGCAGCCATA TGAACAGTCT 
GCCAAGATGA TCTTCATGGA GCTGAATGAT GCATGGTCTG AGTTTGAAAA CCAGGGCTCA 
AGGCCCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
Proceedings of the National Academy of Sciences of the United States of America111(7)2626-31(2014 Feb)
Beilina A,Rudenko IN,Kaganovich A,Civiero L,Chau H,Kalia SK,Kalia LV,Lobbestael E,Chia R,Ndukwe K,Ding J,Nalls MA,,,Olszewski M,Hauser DN,Kumaran R,Lozano AM,Baekelandt V,Greene LE,Taymans JM,Greggio E,Cookson MR

Endocytosis and clathrin-uncoating defects at synapses of auxilin knockout mice.
Proceedings of the National Academy of Sciences of the United States of America107(9)4412-7(2010 Mar)
Yim YI,Sun T,Wu LG,Raimondi A,De Camilli P,Eisenberg E,Greene LE

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Essential role of cyclin-G-associated kinase (Auxilin-2) in developing and mature mice.
Molecular biology of the cell19(7)2766-76(2008 Jul)
Lee DW,Zhao X,Yim YI,Eisenberg E,Greene LE

Multiple roles for cyclin G-associated kinase in clathrin-mediated sorting events.
Traffic (Copenhagen, Denmark)6(12)1103-13(2005 Dec)
Zhang CX,Engqvist-Goldstein AE,Carreno S,Owen DJ,Smythe E,Drubin DG

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Gak cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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