gak cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gak
Entrez Gene ID	100151158
Full Name	cyclin G associated kinase
Synonyms	si:dkeyp-1e8.1
General protein information	Preferred Names cyclin G associated kinase Names cyclin-G-associated kinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301213.3	XP_009299488.1	cyclin-G-associated kinase isoform X1
XM_009301214.2	XP_009299489.1	cyclin-G-associated kinase isoform X2
XM_009301213.2	XP_009299488.1	cyclin-G-associated kinase isoform X1
XM_009301214.3	XP_009299489.1	cyclin-G-associated kinase isoform X2
XM_009301215.2	XP_009299490.1	cyclin-G-associated kinase isoform X3
XM_009301215.3	XP_009299490.1	cyclin-G-associated kinase isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gak	NP_112292.1
Homo sapiens (human)	GAK	NP_005246.2
Bos taurus (cattle)	GAK	NP_001039549.1
Danio rerio (zebrafish)	gak	XP_003198642.2
Pan troglodytes (chimpanzee)	GAK	XP_001140835.1
Mus musculus (house mouse)	Gak	NP_705797.1
Gallus gallus (chicken)	GAK	XP_424873.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003715	XP_310244.7
Macaca mulatta (Rhesus monkey)	GAK	XP_001094120.2
Canis lupus familiaris (dog)	GAK	XP_536279.4
Drosophila melanogaster (fruit fly)	aux	NP_001262243.1
Xenopus tropicalis (tropical clawed frog)	gak	NP_001016350.2
Danio rerio (zebrafish)	LOC100151158	XP_001919224.1

Gene Ontology

The following gak gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gak cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa48972	XM_009301213.3 Latest version!	Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa48973	XM_009301214.3 Latest version!	Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa48974	XM_009301215.3 Latest version!	Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa48972	XM_009301213.2	Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa48973	XM_009301214.2	Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa48974	XM_009301215.2	Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gak ( XM_009301213.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299488.1 Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48972
Clone ID Related Accession (Same CDS sequence)	XM_009301213.3 , XM_009301213.2
Accession Version	XM_009301213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cyclin-G-associated kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCAGTACGA CTGCTAACTT GAGCACTGCT 
GCCAACAAAC CTTCTATGAG TGCCTCAGGA GTCTCGTCTT TCTCCAAGGC CAAATCCCAG 
AGTTTTGACC CTTTCGCAGA CCTGGGAAAT CTCGGCTCAA CACTACCTGG CTCCTCCAGT 
GCTAGCTTTT CTGGCTCAGG GTTTAAAACG GCGCCCTCTG CAGGCGGCCC TAAAAACTCA 
GGTCAGCAGT GGCAGCAAAC ACCTCGACCC TCTTCAGGCC CAAACAAACC CTGGATGCCT 
CCGAACTCCA CGGCCCGGCC ACAAGCTGTT CCTCAAGCAG CCAAACCAAA CTACAACCCC 
AGCTTTAGTG TGATCGGCGG ACGGCAGGAG AGGGGCATCA GGGGGCCCGG ATTTGGTCCA 
AAGCCTAAAG TGAAAGAAGA CGACTTTGAG GATCTTCTGT GCACACAAGG TTTTGGCTCC 
AAACCTGACA GGAAGATGCC CAGAACAATC GCTGAGATGA GGAAGCAGGA GCTGTCCAAA 
GACATGGACC CTCTCAAACT ACAGATCTTG GACTGGATCG AGGGGAAGGA GAGGAACATC 
CGCGCTCTTC TGTCCACGCT TCACACAGTC CTGTGGGAGG GCGAGACCCG CTGGAGACCC 
ATCAACATGG CAGATCTGGT CACTCCAGAT CAGGTCAAGA GGGTCTATAG GAAGGCTGTG 
CTCGTCGTGC ATCCAGACAA GGCCACGGGT CAGCCGTACG AGCAGTACGC TAAGATGATC 
TTCATGGAGC TCAGCGACGC CTGGTCCGAG TTCGAGAACC AAGGATCCAA AGCCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299488.1
CDS	218..4060
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASSSYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTSTTANLSTAANKPSMSASGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301213.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301213.3

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCAGTACGA CTGCTAACTT GAGCACTGCT 
GCCAACAAAC CTTCTATGAG TGCCTCAGGA GTCTCGTCTT TCTCCAAGGC CAAATCCCAG 
AGTTTTGACC CTTTCGCAGA CCTGGGAAAT CTCGGCTCAA CACTACCTGG CTCCTCCAGT 
GCTAGCTTTT CTGGCTCAGG GTTTAAAACG GCGCCCTCTG CAGGCGGCCC TAAAAACTCA 
GGTCAGCAGT GGCAGCAAAC ACCTCGACCC TCTTCAGGCC CAAACAAACC CTGGATGCCT 
CCGAACTCCA CGGCCCGGCC ACAAGCTGTT CCTCAAGCAG CCAAACCAAA CTACAACCCC 
AGCTTTAGTG TGATCGGCGG ACGGCAGGAG AGGGGCATCA GGGGGCCCGG ATTTGGTCCA 
AAGCCTAAAG TGAAAGAAGA CGACTTTGAG GATCTTCTGT GCACACAAGG TTTTGGCTCC 
AAACCTGACA GGAAGATGCC CAGAACAATC GCTGAGATGA GGAAGCAGGA GCTGTCCAAA 
GACATGGACC CTCTCAAACT ACAGATCTTG GACTGGATCG AGGGGAAGGA GAGGAACATC 
CGCGCTCTTC TGTCCACGCT TCACACAGTC CTGTGGGAGG GCGAGACCCG CTGGAGACCC 
ATCAACATGG CAGATCTGGT CACTCCAGAT CAGGTCAAGA GGGTCTATAG GAAGGCTGTG 
CTCGTCGTGC ATCCAGACAA GGCCACGGGT CAGCCGTACG AGCAGTACGC TAAGATGATC 
TTCATGGAGC TCAGCGACGC CTGGTCCGAG TTCGAGAACC AAGGATCCAA AGCCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gak ( XM_009301213.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299488.1 Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48972
Clone ID Related Accession (Same CDS sequence)	XM_009301213.3 , XM_009301213.2
Accession Version	XM_009301213.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cyclin-G-associated kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCAGTACGA CTGCTAACTT GAGCACTGCT 
GCCAACAAAC CTTCTATGAG TGCCTCAGGA GTCTCGTCTT TCTCCAAGGC CAAATCCCAG 
AGTTTTGACC CTTTCGCAGA CCTGGGAAAT CTCGGCTCAA CACTACCTGG CTCCTCCAGT 
GCTAGCTTTT CTGGCTCAGG GTTTAAAACG GCGCCCTCTG CAGGCGGCCC TAAAAACTCA 
GGTCAGCAGT GGCAGCAAAC ACCTCGACCC TCTTCAGGCC CAAACAAACC CTGGATGCCT 
CCGAACTCCA CGGCCCGGCC ACAAGCTGTT CCTCAAGCAG CCAAACCAAA CTACAACCCC 
AGCTTTAGTG TGATCGGCGG ACGGCAGGAG AGGGGCATCA GGGGGCCCGG ATTTGGTCCA 
AAGCCTAAAG TGAAAGAAGA CGACTTTGAG GATCTTCTGT GCACACAAGG TTTTGGCTCC 
AAACCTGACA GGAAGATGCC CAGAACAATC GCTGAGATGA GGAAGCAGGA GCTGTCCAAA 
GACATGGACC CTCTCAAACT ACAGATCTTG GACTGGATCG AGGGGAAGGA GAGGAACATC 
CGCGCTCTTC TGTCCACGCT TCACACAGTC CTGTGGGAGG GCGAGACCCG CTGGAGACCC 
ATCAACATGG CAGATCTGGT CACTCCAGAT CAGGTCAAGA GGGTCTATAG GAAGGCTGTG 
CTCGTCGTGC ATCCAGACAA GGCCACGGGT CAGCCGTACG AGCAGTACGC TAAGATGATC 
TTCATGGAGC TCAGCGACGC CTGGTCCGAG TTCGAGAACC AAGGATCCAA AGCCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299488.1
CDS	210..4052
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASSSYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTSTTANLSTAANKPSMSASGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301213.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301213.2

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCAGTACGA CTGCTAACTT GAGCACTGCT 
GCCAACAAAC CTTCTATGAG TGCCTCAGGA GTCTCGTCTT TCTCCAAGGC CAAATCCCAG 
AGTTTTGACC CTTTCGCAGA CCTGGGAAAT CTCGGCTCAA CACTACCTGG CTCCTCCAGT 
GCTAGCTTTT CTGGCTCAGG GTTTAAAACG GCGCCCTCTG CAGGCGGCCC TAAAAACTCA 
GGTCAGCAGT GGCAGCAAAC ACCTCGACCC TCTTCAGGCC CAAACAAACC CTGGATGCCT 
CCGAACTCCA CGGCCCGGCC ACAAGCTGTT CCTCAAGCAG CCAAACCAAA CTACAACCCC 
AGCTTTAGTG TGATCGGCGG ACGGCAGGAG AGGGGCATCA GGGGGCCCGG ATTTGGTCCA 
AAGCCTAAAG TGAAAGAAGA CGACTTTGAG GATCTTCTGT GCACACAAGG TTTTGGCTCC 
AAACCTGACA GGAAGATGCC CAGAACAATC GCTGAGATGA GGAAGCAGGA GCTGTCCAAA 
GACATGGACC CTCTCAAACT ACAGATCTTG GACTGGATCG AGGGGAAGGA GAGGAACATC 
CGCGCTCTTC TGTCCACGCT TCACACAGTC CTGTGGGAGG GCGAGACCCG CTGGAGACCC 
ATCAACATGG CAGATCTGGT CACTCCAGAT CAGGTCAAGA GGGTCTATAG GAAGGCTGTG 
CTCGTCGTGC ATCCAGACAA GGCCACGGGT CAGCCGTACG AGCAGTACGC TAAGATGATC 
TTCATGGAGC TCAGCGACGC CTGGTCCGAG TTCGAGAACC AAGGATCCAA AGCCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gak ( XM_009301214.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299489.1 Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.

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CloneID	ODa48973
Clone ID Related Accession (Same CDS sequence)	XM_009301214.2 , XM_009301214.3
Accession Version	XM_009301214.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cyclin-G-associated kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301214.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGTTA TGCTAAAGGA 
GACCTGGACC TGTCCTACAT CACCTCCAGA CTAGCAGTCA TGTCTTTCCC AGCTGAAGGT 
GTTGAATCTG CTATTAAGAA TAACATTGAG GATGTGCGTC TGTTCCTGGA CTCTCGACAC 
GCGGGTCATT ACGCCGTTTA TAACCTGTCC AAACGCTCCT ACAGACCAGC CAGGTTTCAC 
AACAGGGTAT CCGAGTGCGG TTGGCAGCCA AGGCGAGCTC CTACACTGAA GAACTTGTAC 
AGTATATGTA AAAACATGCA TCAGTGGCTC AAACAGGACC AGAGGAACAT CTGCATCGTA 
CACTGTCTGG ACGGACGGGC AGCGTCTGCT GTGGTGGTTT GTGCCTTCCT CTGTTTCTGT 
CGTCTCTTCT CCACCGCTGA GGCTGCAGTT TACATGTTCA GCATGAAGCG ATGTCCACCG 
GGCATCTCAG CCTCACACAA AAGGTATATT GAATACATGT GTGATATGAT GGCAGAAGAG 
CCCATAATCC CCCACAGCAA GCCTATAACC ATTCGTTCAG TAGCCATGAC GCCCGTACCG 
CTCTTTAATA AGCAGAGGAA TGGCTGTCGG CCGTTCTGTG AGGTCTATGT TGGAGATGAG 
CGTGTCGCCA CCACCTCACA GGAGTACGAC AGAATGAGGG ATTTTAAGAT GGAGGACGGG 
CGTGCGGAGA TTCCTCTGAA TATCACCGTG CAGGGTGACG TTTTGATTGT GATTTATCAT 
GCACGATCCA CGCTCGGCGG ACGCCTGCAG GCCAAAATGG CATCCATGAA AATGTTTCAG 
ATTCAGTTTC ATACAGGCTT TGTGCCCAGA AATGCCTCCA CTGTCAAATT TGCAAAGTAT 
GATCTGGACG CGTGTGACAT CCAGGAGAAA TATCCTGACC TCTTCCAGGT GCACGTCAAT 
GTGGAGGTGG AGCCTCAGGA TCGTCCCAGC AACAAGACTC CACCCTGGGA GAACTTCGCC 
ACCAAAGGAC TCAATCCCAA GATCCTCTTC TCCAGCCGAG ATGAACAGCA GGAGATTCTG 
ACTAAATTTG GTAAACCAGA GCTTCCTCGT CAGCCTGGTT CGACAGCCTG TTATGCCTCT 
GACCCACCGC AGTCGGAGAA CTCTCCAGAT GACCCGCAGA CTGAGTCGGA CTCACCGTCA 
CCCCAGAGCA CAGACACCAA CGCCAACAAC TTCTTTCAGA CGCTGGACTG GGAAGACACA 
AGTCGACACT TTGACCACGC TGACGGCCAC TTGGATGACC AGGAAGATTT GAGCGGCGGC 
TCGGAGGAGG AGTCAGTGTC GTACTCTGCC CCTATTGAAG GGACTCTGTC ACGGGACACC 
AGTGAGCCGC TGTTTGAAGC AGACTTTACA AACACTCCGC CTGAAGCTCA ACCCGACACT 
GAGGACCTGC TGGGTCTGAA CTCTGAGCCC GGTCCTGCTG TTCCAGAGAG CGGTTTGAAG 
AGCTCCGCCA GTAACAGTAA TTTACTGAAC GATCTACTGT TCGCCCCTCA GACCAACACT 
GTGCCTTCAG CGAGCAATGA AGACCTGATG GGGGAAGATG CGGGAGAAGA TCTCTTCTTT 
TCCAACTCGT CCAGCCAGCC GCCACCAGCC ACTAATAAAG ATTTCTTCGA CCCATTCGGC 
GTGGGTTCGG CCTCCTCAGG GTCTGATTTG TTTGGTGATC TGCTGGGTTC AGATGCTGCT 
AACAGTGGCT TCAAAGCTCC AGCTCCTGCA TCAAACTCCA GCCTCTTCAA CCTCAACAAG 
TTAGCAAGTG AAACCCCGAA GATGACCTCA TCAGCGAGTC AGCCTGATCT GTTGAGTGGG 
TGGGACAGCT GGGCCACCAG CGGAACCAGT ACGACTGCTA ACTTGAGCAC TGCTGCCAAC 
AAACCTTCTA TGAGTGCCTC AGGAGTCTCG TCTTTCTCCA AGGCCAAATC CCAGAGTTTT 
GACCCTTTCG CAGACCTGGG AAATCTCGGC TCAACACTAC CTGGCTCCTC CAGTGCTAGC 
TTTTCTGGCT CAGGGTTTAA AACGGCGCCC TCTGCAGGCG GCCCTAAAAA CTCAGGTCAG 
CAGTGGCAGC AAACACCTCG ACCCTCTTCA GGCCCAAACA AACCCTGGAT GCCTCCGAAC 
TCCACGGCCC GGCCACAAGC TGTTCCTCAA GCAGCCAAAC CAAACTACAA CCCCAGCTTT 
AGTGTGATCG GCGGACGGCA GGAGAGGGGC ATCAGGGGGC CCGGATTTGG TCCAAAGCCT 
AAAGTGAAAG AAGACGACTT TGAGGATCTT CTGTGCACAC AAGGTTTTGG CTCCAAACCT 
GACAGGAAGA TGCCCAGAAC AATCGCTGAG ATGAGGAAGC AGGAGCTGTC CAAAGACATG 
GACCCTCTCA AACTACAGAT CTTGGACTGG ATCGAGGGGA AGGAGAGGAA CATCCGCGCT 
CTTCTGTCCA CGCTTCACAC AGTCCTGTGG GAGGGCGAGA CCCGCTGGAG ACCCATCAAC 
ATGGCAGATC TGGTCACTCC AGATCAGGTC AAGAGGGTCT ATAGGAAGGC TGTGCTCGTC 
GTGCATCCAG ACAAGGCCAC GGGTCAGCCG TACGAGCAGT ACGCTAAGAT GATCTTCATG 
GAGCTCAGCG ACGCCTGGTC CGAGTTCGAG AACCAAGGAT CCAAAGCCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299489.1
CDS	210..4046
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTSTTANLSTAANKPSMSASGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301214.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301214.2

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGTTA TGCTAAAGGA 
GACCTGGACC TGTCCTACAT CACCTCCAGA CTAGCAGTCA TGTCTTTCCC AGCTGAAGGT 
GTTGAATCTG CTATTAAGAA TAACATTGAG GATGTGCGTC TGTTCCTGGA CTCTCGACAC 
GCGGGTCATT ACGCCGTTTA TAACCTGTCC AAACGCTCCT ACAGACCAGC CAGGTTTCAC 
AACAGGGTAT CCGAGTGCGG TTGGCAGCCA AGGCGAGCTC CTACACTGAA GAACTTGTAC 
AGTATATGTA AAAACATGCA TCAGTGGCTC AAACAGGACC AGAGGAACAT CTGCATCGTA 
CACTGTCTGG ACGGACGGGC AGCGTCTGCT GTGGTGGTTT GTGCCTTCCT CTGTTTCTGT 
CGTCTCTTCT CCACCGCTGA GGCTGCAGTT TACATGTTCA GCATGAAGCG ATGTCCACCG 
GGCATCTCAG CCTCACACAA AAGGTATATT GAATACATGT GTGATATGAT GGCAGAAGAG 
CCCATAATCC CCCACAGCAA GCCTATAACC ATTCGTTCAG TAGCCATGAC GCCCGTACCG 
CTCTTTAATA AGCAGAGGAA TGGCTGTCGG CCGTTCTGTG AGGTCTATGT TGGAGATGAG 
CGTGTCGCCA CCACCTCACA GGAGTACGAC AGAATGAGGG ATTTTAAGAT GGAGGACGGG 
CGTGCGGAGA TTCCTCTGAA TATCACCGTG CAGGGTGACG TTTTGATTGT GATTTATCAT 
GCACGATCCA CGCTCGGCGG ACGCCTGCAG GCCAAAATGG CATCCATGAA AATGTTTCAG 
ATTCAGTTTC ATACAGGCTT TGTGCCCAGA AATGCCTCCA CTGTCAAATT TGCAAAGTAT 
GATCTGGACG CGTGTGACAT CCAGGAGAAA TATCCTGACC TCTTCCAGGT GCACGTCAAT 
GTGGAGGTGG AGCCTCAGGA TCGTCCCAGC AACAAGACTC CACCCTGGGA GAACTTCGCC 
ACCAAAGGAC TCAATCCCAA GATCCTCTTC TCCAGCCGAG ATGAACAGCA GGAGATTCTG 
ACTAAATTTG GTAAACCAGA GCTTCCTCGT CAGCCTGGTT CGACAGCCTG TTATGCCTCT 
GACCCACCGC AGTCGGAGAA CTCTCCAGAT GACCCGCAGA CTGAGTCGGA CTCACCGTCA 
CCCCAGAGCA CAGACACCAA CGCCAACAAC TTCTTTCAGA CGCTGGACTG GGAAGACACA 
AGTCGACACT TTGACCACGC TGACGGCCAC TTGGATGACC AGGAAGATTT GAGCGGCGGC 
TCGGAGGAGG AGTCAGTGTC GTACTCTGCC CCTATTGAAG GGACTCTGTC ACGGGACACC 
AGTGAGCCGC TGTTTGAAGC AGACTTTACA AACACTCCGC CTGAAGCTCA ACCCGACACT 
GAGGACCTGC TGGGTCTGAA CTCTGAGCCC GGTCCTGCTG TTCCAGAGAG CGGTTTGAAG 
AGCTCCGCCA GTAACAGTAA TTTACTGAAC GATCTACTGT TCGCCCCTCA GACCAACACT 
GTGCCTTCAG CGAGCAATGA AGACCTGATG GGGGAAGATG CGGGAGAAGA TCTCTTCTTT 
TCCAACTCGT CCAGCCAGCC GCCACCAGCC ACTAATAAAG ATTTCTTCGA CCCATTCGGC 
GTGGGTTCGG CCTCCTCAGG GTCTGATTTG TTTGGTGATC TGCTGGGTTC AGATGCTGCT 
AACAGTGGCT TCAAAGCTCC AGCTCCTGCA TCAAACTCCA GCCTCTTCAA CCTCAACAAG 
TTAGCAAGTG AAACCCCGAA GATGACCTCA TCAGCGAGTC AGCCTGATCT GTTGAGTGGG 
TGGGACAGCT GGGCCACCAG CGGAACCAGT ACGACTGCTA ACTTGAGCAC TGCTGCCAAC 
AAACCTTCTA TGAGTGCCTC AGGAGTCTCG TCTTTCTCCA AGGCCAAATC CCAGAGTTTT 
GACCCTTTCG CAGACCTGGG AAATCTCGGC TCAACACTAC CTGGCTCCTC CAGTGCTAGC 
TTTTCTGGCT CAGGGTTTAA AACGGCGCCC TCTGCAGGCG GCCCTAAAAA CTCAGGTCAG 
CAGTGGCAGC AAACACCTCG ACCCTCTTCA GGCCCAAACA AACCCTGGAT GCCTCCGAAC 
TCCACGGCCC GGCCACAAGC TGTTCCTCAA GCAGCCAAAC CAAACTACAA CCCCAGCTTT 
AGTGTGATCG GCGGACGGCA GGAGAGGGGC ATCAGGGGGC CCGGATTTGG TCCAAAGCCT 
AAAGTGAAAG AAGACGACTT TGAGGATCTT CTGTGCACAC AAGGTTTTGG CTCCAAACCT 
GACAGGAAGA TGCCCAGAAC AATCGCTGAG ATGAGGAAGC AGGAGCTGTC CAAAGACATG 
GACCCTCTCA AACTACAGAT CTTGGACTGG ATCGAGGGGA AGGAGAGGAA CATCCGCGCT 
CTTCTGTCCA CGCTTCACAC AGTCCTGTGG GAGGGCGAGA CCCGCTGGAG ACCCATCAAC 
ATGGCAGATC TGGTCACTCC AGATCAGGTC AAGAGGGTCT ATAGGAAGGC TGTGCTCGTC 
GTGCATCCAG ACAAGGCCAC GGGTCAGCCG TACGAGCAGT ACGCTAAGAT GATCTTCATG 
GAGCTCAGCG ACGCCTGGTC CGAGTTCGAG AACCAAGGAT CCAAAGCCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gak ( XM_009301214.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299489.1 Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa48973
Clone ID Related Accession (Same CDS sequence)	XM_009301214.2 , XM_009301214.3
Accession Version	XM_009301214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cyclin-G-associated kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGTTA TGCTAAAGGA 
GACCTGGACC TGTCCTACAT CACCTCCAGA CTAGCAGTCA TGTCTTTCCC AGCTGAAGGT 
GTTGAATCTG CTATTAAGAA TAACATTGAG GATGTGCGTC TGTTCCTGGA CTCTCGACAC 
GCGGGTCATT ACGCCGTTTA TAACCTGTCC AAACGCTCCT ACAGACCAGC CAGGTTTCAC 
AACAGGGTAT CCGAGTGCGG TTGGCAGCCA AGGCGAGCTC CTACACTGAA GAACTTGTAC 
AGTATATGTA AAAACATGCA TCAGTGGCTC AAACAGGACC AGAGGAACAT CTGCATCGTA 
CACTGTCTGG ACGGACGGGC AGCGTCTGCT GTGGTGGTTT GTGCCTTCCT CTGTTTCTGT 
CGTCTCTTCT CCACCGCTGA GGCTGCAGTT TACATGTTCA GCATGAAGCG ATGTCCACCG 
GGCATCTCAG CCTCACACAA AAGGTATATT GAATACATGT GTGATATGAT GGCAGAAGAG 
CCCATAATCC CCCACAGCAA GCCTATAACC ATTCGTTCAG TAGCCATGAC GCCCGTACCG 
CTCTTTAATA AGCAGAGGAA TGGCTGTCGG CCGTTCTGTG AGGTCTATGT TGGAGATGAG 
CGTGTCGCCA CCACCTCACA GGAGTACGAC AGAATGAGGG ATTTTAAGAT GGAGGACGGG 
CGTGCGGAGA TTCCTCTGAA TATCACCGTG CAGGGTGACG TTTTGATTGT GATTTATCAT 
GCACGATCCA CGCTCGGCGG ACGCCTGCAG GCCAAAATGG CATCCATGAA AATGTTTCAG 
ATTCAGTTTC ATACAGGCTT TGTGCCCAGA AATGCCTCCA CTGTCAAATT TGCAAAGTAT 
GATCTGGACG CGTGTGACAT CCAGGAGAAA TATCCTGACC TCTTCCAGGT GCACGTCAAT 
GTGGAGGTGG AGCCTCAGGA TCGTCCCAGC AACAAGACTC CACCCTGGGA GAACTTCGCC 
ACCAAAGGAC TCAATCCCAA GATCCTCTTC TCCAGCCGAG ATGAACAGCA GGAGATTCTG 
ACTAAATTTG GTAAACCAGA GCTTCCTCGT CAGCCTGGTT CGACAGCCTG TTATGCCTCT 
GACCCACCGC AGTCGGAGAA CTCTCCAGAT GACCCGCAGA CTGAGTCGGA CTCACCGTCA 
CCCCAGAGCA CAGACACCAA CGCCAACAAC TTCTTTCAGA CGCTGGACTG GGAAGACACA 
AGTCGACACT TTGACCACGC TGACGGCCAC TTGGATGACC AGGAAGATTT GAGCGGCGGC 
TCGGAGGAGG AGTCAGTGTC GTACTCTGCC CCTATTGAAG GGACTCTGTC ACGGGACACC 
AGTGAGCCGC TGTTTGAAGC AGACTTTACA AACACTCCGC CTGAAGCTCA ACCCGACACT 
GAGGACCTGC TGGGTCTGAA CTCTGAGCCC GGTCCTGCTG TTCCAGAGAG CGGTTTGAAG 
AGCTCCGCCA GTAACAGTAA TTTACTGAAC GATCTACTGT TCGCCCCTCA GACCAACACT 
GTGCCTTCAG CGAGCAATGA AGACCTGATG GGGGAAGATG CGGGAGAAGA TCTCTTCTTT 
TCCAACTCGT CCAGCCAGCC GCCACCAGCC ACTAATAAAG ATTTCTTCGA CCCATTCGGC 
GTGGGTTCGG CCTCCTCAGG GTCTGATTTG TTTGGTGATC TGCTGGGTTC AGATGCTGCT 
AACAGTGGCT TCAAAGCTCC AGCTCCTGCA TCAAACTCCA GCCTCTTCAA CCTCAACAAG 
TTAGCAAGTG AAACCCCGAA GATGACCTCA TCAGCGAGTC AGCCTGATCT GTTGAGTGGG 
TGGGACAGCT GGGCCACCAG CGGAACCAGT ACGACTGCTA ACTTGAGCAC TGCTGCCAAC 
AAACCTTCTA TGAGTGCCTC AGGAGTCTCG TCTTTCTCCA AGGCCAAATC CCAGAGTTTT 
GACCCTTTCG CAGACCTGGG AAATCTCGGC TCAACACTAC CTGGCTCCTC CAGTGCTAGC 
TTTTCTGGCT CAGGGTTTAA AACGGCGCCC TCTGCAGGCG GCCCTAAAAA CTCAGGTCAG 
CAGTGGCAGC AAACACCTCG ACCCTCTTCA GGCCCAAACA AACCCTGGAT GCCTCCGAAC 
TCCACGGCCC GGCCACAAGC TGTTCCTCAA GCAGCCAAAC CAAACTACAA CCCCAGCTTT 
AGTGTGATCG GCGGACGGCA GGAGAGGGGC ATCAGGGGGC CCGGATTTGG TCCAAAGCCT 
AAAGTGAAAG AAGACGACTT TGAGGATCTT CTGTGCACAC AAGGTTTTGG CTCCAAACCT 
GACAGGAAGA TGCCCAGAAC AATCGCTGAG ATGAGGAAGC AGGAGCTGTC CAAAGACATG 
GACCCTCTCA AACTACAGAT CTTGGACTGG ATCGAGGGGA AGGAGAGGAA CATCCGCGCT 
CTTCTGTCCA CGCTTCACAC AGTCCTGTGG GAGGGCGAGA CCCGCTGGAG ACCCATCAAC 
ATGGCAGATC TGGTCACTCC AGATCAGGTC AAGAGGGTCT ATAGGAAGGC TGTGCTCGTC 
GTGCATCCAG ACAAGGCCAC GGGTCAGCCG TACGAGCAGT ACGCTAAGAT GATCTTCATG 
GAGCTCAGCG ACGCCTGGTC CGAGTTCGAG AACCAAGGAT CCAAAGCCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299489.1
CDS	218..4054
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTSTTANLSTAANKPSMSASGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301214.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301214.3

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGTTA TGCTAAAGGA 
GACCTGGACC TGTCCTACAT CACCTCCAGA CTAGCAGTCA TGTCTTTCCC AGCTGAAGGT 
GTTGAATCTG CTATTAAGAA TAACATTGAG GATGTGCGTC TGTTCCTGGA CTCTCGACAC 
GCGGGTCATT ACGCCGTTTA TAACCTGTCC AAACGCTCCT ACAGACCAGC CAGGTTTCAC 
AACAGGGTAT CCGAGTGCGG TTGGCAGCCA AGGCGAGCTC CTACACTGAA GAACTTGTAC 
AGTATATGTA AAAACATGCA TCAGTGGCTC AAACAGGACC AGAGGAACAT CTGCATCGTA 
CACTGTCTGG ACGGACGGGC AGCGTCTGCT GTGGTGGTTT GTGCCTTCCT CTGTTTCTGT 
CGTCTCTTCT CCACCGCTGA GGCTGCAGTT TACATGTTCA GCATGAAGCG ATGTCCACCG 
GGCATCTCAG CCTCACACAA AAGGTATATT GAATACATGT GTGATATGAT GGCAGAAGAG 
CCCATAATCC CCCACAGCAA GCCTATAACC ATTCGTTCAG TAGCCATGAC GCCCGTACCG 
CTCTTTAATA AGCAGAGGAA TGGCTGTCGG CCGTTCTGTG AGGTCTATGT TGGAGATGAG 
CGTGTCGCCA CCACCTCACA GGAGTACGAC AGAATGAGGG ATTTTAAGAT GGAGGACGGG 
CGTGCGGAGA TTCCTCTGAA TATCACCGTG CAGGGTGACG TTTTGATTGT GATTTATCAT 
GCACGATCCA CGCTCGGCGG ACGCCTGCAG GCCAAAATGG CATCCATGAA AATGTTTCAG 
ATTCAGTTTC ATACAGGCTT TGTGCCCAGA AATGCCTCCA CTGTCAAATT TGCAAAGTAT 
GATCTGGACG CGTGTGACAT CCAGGAGAAA TATCCTGACC TCTTCCAGGT GCACGTCAAT 
GTGGAGGTGG AGCCTCAGGA TCGTCCCAGC AACAAGACTC CACCCTGGGA GAACTTCGCC 
ACCAAAGGAC TCAATCCCAA GATCCTCTTC TCCAGCCGAG ATGAACAGCA GGAGATTCTG 
ACTAAATTTG GTAAACCAGA GCTTCCTCGT CAGCCTGGTT CGACAGCCTG TTATGCCTCT 
GACCCACCGC AGTCGGAGAA CTCTCCAGAT GACCCGCAGA CTGAGTCGGA CTCACCGTCA 
CCCCAGAGCA CAGACACCAA CGCCAACAAC TTCTTTCAGA CGCTGGACTG GGAAGACACA 
AGTCGACACT TTGACCACGC TGACGGCCAC TTGGATGACC AGGAAGATTT GAGCGGCGGC 
TCGGAGGAGG AGTCAGTGTC GTACTCTGCC CCTATTGAAG GGACTCTGTC ACGGGACACC 
AGTGAGCCGC TGTTTGAAGC AGACTTTACA AACACTCCGC CTGAAGCTCA ACCCGACACT 
GAGGACCTGC TGGGTCTGAA CTCTGAGCCC GGTCCTGCTG TTCCAGAGAG CGGTTTGAAG 
AGCTCCGCCA GTAACAGTAA TTTACTGAAC GATCTACTGT TCGCCCCTCA GACCAACACT 
GTGCCTTCAG CGAGCAATGA AGACCTGATG GGGGAAGATG CGGGAGAAGA TCTCTTCTTT 
TCCAACTCGT CCAGCCAGCC GCCACCAGCC ACTAATAAAG ATTTCTTCGA CCCATTCGGC 
GTGGGTTCGG CCTCCTCAGG GTCTGATTTG TTTGGTGATC TGCTGGGTTC AGATGCTGCT 
AACAGTGGCT TCAAAGCTCC AGCTCCTGCA TCAAACTCCA GCCTCTTCAA CCTCAACAAG 
TTAGCAAGTG AAACCCCGAA GATGACCTCA TCAGCGAGTC AGCCTGATCT GTTGAGTGGG 
TGGGACAGCT GGGCCACCAG CGGAACCAGT ACGACTGCTA ACTTGAGCAC TGCTGCCAAC 
AAACCTTCTA TGAGTGCCTC AGGAGTCTCG TCTTTCTCCA AGGCCAAATC CCAGAGTTTT 
GACCCTTTCG CAGACCTGGG AAATCTCGGC TCAACACTAC CTGGCTCCTC CAGTGCTAGC 
TTTTCTGGCT CAGGGTTTAA AACGGCGCCC TCTGCAGGCG GCCCTAAAAA CTCAGGTCAG 
CAGTGGCAGC AAACACCTCG ACCCTCTTCA GGCCCAAACA AACCCTGGAT GCCTCCGAAC 
TCCACGGCCC GGCCACAAGC TGTTCCTCAA GCAGCCAAAC CAAACTACAA CCCCAGCTTT 
AGTGTGATCG GCGGACGGCA GGAGAGGGGC ATCAGGGGGC CCGGATTTGG TCCAAAGCCT 
AAAGTGAAAG AAGACGACTT TGAGGATCTT CTGTGCACAC AAGGTTTTGG CTCCAAACCT 
GACAGGAAGA TGCCCAGAAC AATCGCTGAG ATGAGGAAGC AGGAGCTGTC CAAAGACATG 
GACCCTCTCA AACTACAGAT CTTGGACTGG ATCGAGGGGA AGGAGAGGAA CATCCGCGCT 
CTTCTGTCCA CGCTTCACAC AGTCCTGTGG GAGGGCGAGA CCCGCTGGAG ACCCATCAAC 
ATGGCAGATC TGGTCACTCC AGATCAGGTC AAGAGGGTCT ATAGGAAGGC TGTGCTCGTC 
GTGCATCCAG ACAAGGCCAC GGGTCAGCCG TACGAGCAGT ACGCTAAGAT GATCTTCATG 
GAGCTCAGCG ACGCCTGGTC CGAGTTCGAG AACCAAGGAT CCAAAGCCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gak ( XM_009301215.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299490.1 Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48974
Clone ID Related Accession (Same CDS sequence)	XM_009301215.2 , XM_009301215.3
Accession Version	XM_009301215.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	cyclin-G-associated kinase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCACCTCAG GAGTCTCGTC TTTCTCCAAG 
GCCAAATCCC AGAGTTTTGA CCCTTTCGCA GACCTGGGAA ATCTCGGCTC AACACTACCT 
GGCTCCTCCA GTGCTAGCTT TTCTGGCTCA GGGTTTAAAA CGGCGCCCTC TGCAGGCGGC 
CCTAAAAACT CAGGTCAGCA GTGGCAGCAA ACACCTCGAC CCTCTTCAGG CCCAAACAAA 
CCCTGGATGC CTCCGAACTC CACGGCCCGG CCACAAGCTG TTCCTCAAGC AGCCAAACCA 
AACTACAACC CCAGCTTTAG TGTGATCGGC GGACGGCAGG AGAGGGGCAT CAGGGGGCCC 
GGATTTGGTC CAAAGCCTAA AGTGAAAGAA GACGACTTTG AGGATCTTCT GTGCACACAA 
GGTTTTGGCT CCAAACCTGA CAGGAAGATG CCCAGAACAA TCGCTGAGAT GAGGAAGCAG 
GAGCTGTCCA AAGACATGGA CCCTCTCAAA CTACAGATCT TGGACTGGAT CGAGGGGAAG 
GAGAGGAACA TCCGCGCTCT TCTGTCCACG CTTCACACAG TCCTGTGGGA GGGCGAGACC 
CGCTGGAGAC CCATCAACAT GGCAGATCTG GTCACTCCAG ATCAGGTCAA GAGGGTCTAT 
AGGAAGGCTG TGCTCGTCGT GCATCCAGAC AAGGCCACGG GTCAGCCGTA CGAGCAGTAC 
GCTAAGATGA TCTTCATGGA GCTCAGCGAC GCCTGGTCCG AGTTCGAGAA CCAAGGATCC 
AAAGCCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299490.1
CDS	210..4004
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASSSYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTTSGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301215.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301215.2

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCACCTCAG GAGTCTCGTC TTTCTCCAAG 
GCCAAATCCC AGAGTTTTGA CCCTTTCGCA GACCTGGGAA ATCTCGGCTC AACACTACCT 
GGCTCCTCCA GTGCTAGCTT TTCTGGCTCA GGGTTTAAAA CGGCGCCCTC TGCAGGCGGC 
CCTAAAAACT CAGGTCAGCA GTGGCAGCAA ACACCTCGAC CCTCTTCAGG CCCAAACAAA 
CCCTGGATGC CTCCGAACTC CACGGCCCGG CCACAAGCTG TTCCTCAAGC AGCCAAACCA 
AACTACAACC CCAGCTTTAG TGTGATCGGC GGACGGCAGG AGAGGGGCAT CAGGGGGCCC 
GGATTTGGTC CAAAGCCTAA AGTGAAAGAA GACGACTTTG AGGATCTTCT GTGCACACAA 
GGTTTTGGCT CCAAACCTGA CAGGAAGATG CCCAGAACAA TCGCTGAGAT GAGGAAGCAG 
GAGCTGTCCA AAGACATGGA CCCTCTCAAA CTACAGATCT TGGACTGGAT CGAGGGGAAG 
GAGAGGAACA TCCGCGCTCT TCTGTCCACG CTTCACACAG TCCTGTGGGA GGGCGAGACC 
CGCTGGAGAC CCATCAACAT GGCAGATCTG GTCACTCCAG ATCAGGTCAA GAGGGTCTAT 
AGGAAGGCTG TGCTCGTCGT GCATCCAGAC AAGGCCACGG GTCAGCCGTA CGAGCAGTAC 
GCTAAGATGA TCTTCATGGA GCTCAGCGAC GCCTGGTCCG AGTTCGAGAA CCAAGGATCC 
AAAGCCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gak ( XM_009301215.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299490.1 Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.

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CloneID	ODa48974
Clone ID Related Accession (Same CDS sequence)	XM_009301215.2 , XM_009301215.3
Accession Version	XM_009301215.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	cyclin-G-associated kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301215.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCACCTCAG GAGTCTCGTC TTTCTCCAAG 
GCCAAATCCC AGAGTTTTGA CCCTTTCGCA GACCTGGGAA ATCTCGGCTC AACACTACCT 
GGCTCCTCCA GTGCTAGCTT TTCTGGCTCA GGGTTTAAAA CGGCGCCCTC TGCAGGCGGC 
CCTAAAAACT CAGGTCAGCA GTGGCAGCAA ACACCTCGAC CCTCTTCAGG CCCAAACAAA 
CCCTGGATGC CTCCGAACTC CACGGCCCGG CCACAAGCTG TTCCTCAAGC AGCCAAACCA 
AACTACAACC CCAGCTTTAG TGTGATCGGC GGACGGCAGG AGAGGGGCAT CAGGGGGCCC 
GGATTTGGTC CAAAGCCTAA AGTGAAAGAA GACGACTTTG AGGATCTTCT GTGCACACAA 
GGTTTTGGCT CCAAACCTGA CAGGAAGATG CCCAGAACAA TCGCTGAGAT GAGGAAGCAG 
GAGCTGTCCA AAGACATGGA CCCTCTCAAA CTACAGATCT TGGACTGGAT CGAGGGGAAG 
GAGAGGAACA TCCGCGCTCT TCTGTCCACG CTTCACACAG TCCTGTGGGA GGGCGAGACC 
CGCTGGAGAC CCATCAACAT GGCAGATCTG GTCACTCCAG ATCAGGTCAA GAGGGTCTAT 
AGGAAGGCTG TGCTCGTCGT GCATCCAGAC AAGGCCACGG GTCAGCCGTA CGAGCAGTAC 
GCTAAGATGA TCTTCATGGA GCTCAGCGAC GCCTGGTCCG AGTTCGAGAA CCAAGGATCC 
AAAGCCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299490.1
CDS	218..4012
Translation	MSLFQSALDFLAGPGGSGGAASRDQNDFVGQVVELGELKLRIKRVIAEGGFAFVYEAQDLGSSKDYALKRLLSNEEEKNKAIIQEVCFMKKLSGHPNVVQFCSAASISKEESDTGQAEFLILTELCRGQLVDFVKKVEQKGPMSCDTVLKIFYQSCRAVQHMHKQSPAVIHRDLKIENLLISHQGTIKLCDFGSATTLAHYPDYSWSAHKRSMVEDEITRNTTPAYRTPEMIDLYSNYPINEKQDIWALGCILYLLCFKQHPFEEGAKLQIVNGKYSIPQNDTKYTVFHQLIRSMLKINPEERLSINELVNQLQEIAAARNVNPKSPITELLEQNGGFGNNSAQLPSQINNIHNSGVYDTDPTMGGGFLDILKGGTERFLTNIKDTSSKVIQSVASSSYAKGDLDLSYITSRLAVMSFPAEGVESAIKNNIEDVRLFLDSRHAGHYAVYNLSKRSYRPARFHNRVSECGWQPRRAPTLKNLYSICKNMHQWLKQDQRNICIVHCLDGRAASAVVVCAFLCFCRLFSTAEAAVYMFSMKRCPPGISASHKRYIEYMCDMMAEEPIIPHSKPITIRSVAMTPVPLFNKQRNGCRPFCEVYVGDERVATTSQEYDRMRDFKMEDGRAEIPLNITVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNASTVKFAKYDLDACDIQEKYPDLFQVHVNVEVEPQDRPSNKTPPWENFATKGLNPKILFSSRDEQQEILTKFGKPELPRQPGSTACYASDPPQSENSPDDPQTESDSPSPQSTDTNANNFFQTLDWEDTSRHFDHADGHLDDQEDLSGGSEEESVSYSAPIEGTLSRDTSEPLFEADFTNTPPEAQPDTEDLLGLNSEPGPAVPESGLKSSASNSNLLNDLLFAPQTNTVPSASNEDLMGEDAGEDLFFSNSSSQPPPATNKDFFDPFGVGSASSGSDLFGDLLGSDAANSGFKAPAPASNSSLFNLNKLASETPKMTSSASQPDLLSGWDSWATSGTTSGVSSFSKAKSQSFDPFADLGNLGSTLPGSSSASFSGSGFKTAPSAGGPKNSGQQWQQTPRPSSGPNKPWMPPNSTARPQAVPQAAKPNYNPSFSVIGGRQERGIRGPGFGPKPKVKEDDFEDLLCTQGFGSKPDRKMPRTIAEMRKQELSKDMDPLKLQILDWIEGKERNIRALLSTLHTVLWEGETRWRPINMADLVTPDQVKRVYRKAVLVVHPDKATGQPYEQYAKMIFMELSDAWSEFENQGSKALF

Target ORF information:

RefSeq Version	XM_009301215.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cyclin G associated kinase (gak), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301215.3

ORF Insert Sequence:

ATGTCCCTGT TCCAGTCAGC GCTCGATTTC CTGGCCGGGC CCGGCGGATC CGGTGGCGCC 
GCTAGCCGGG ATCAGAACGA TTTCGTCGGG CAGGTGGTGG AGCTCGGAGA GCTGAAGCTC 
CGGATCAAAC GAGTCATCGC GGAAGGTGGA TTTGCATTCG TGTATGAAGC CCAGGATTTA 
GGAAGCAGTA AGGACTATGC GCTCAAGAGG CTTCTGTCCA ACGAAGAGGA AAAGAACAAG 
GCCATCATTC AGGAAGTCTG CTTCATGAAA AAGCTGTCTG GTCATCCTAA TGTTGTACAG 
TTCTGCTCCG CTGCGTCCAT CAGTAAAGAA GAGTCTGACA CGGGCCAGGC CGAGTTCCTC 
ATCCTAACGG AGCTCTGTAG AGGCCAGCTG GTGGATTTCG TCAAGAAGGT GGAGCAGAAA 
GGACCGATGT CATGTGACAC AGTGCTGAAG ATCTTTTATC AGTCTTGTCG GGCCGTGCAG 
CACATGCACA AACAGTCTCC AGCAGTCATA CACCGAGACC TGAAGATTGA GAATTTGTTG 
ATCAGTCACC AGGGCACCAT AAAGCTTTGT GACTTTGGCA GCGCGACTAC ATTGGCACAT 
TATCCAGACT ACAGCTGGTC TGCGCACAAG AGATCTATGG TGGAAGATGA GATAACAAGA 
AACACAACTC CAGCGTACAG GACACCAGAG ATGATCGACC TGTATTCTAA CTACCCAATA 
AATGAGAAGC AGGACATATG GGCTCTTGGT TGCATCCTAT ACCTGCTGTG CTTTAAGCAG 
CACCCGTTTG AAGAGGGAGC CAAACTGCAG ATCGTAAACG GGAAATACAG CATTCCTCAA 
AATGACACCA AGTACACAGT CTTTCACCAG CTTATACGAT CAATGTTGAA AATCAACCCT 
GAAGAGCGGC TGTCTATCAA CGAGCTTGTT AACCAACTAC AGGAAATTGC TGCAGCGAGA 
AATGTCAACC CCAAATCACC CATTACTGAG CTCCTGGAGC AGAACGGAGG ATTCGGGAAC 
AACAGCGCGC AGCTTCCCTC ACAGATCAAC AACATACACA ACTCTGGTGT GTATGACACA 
GACCCGACCA TGGGTGGAGG ATTCCTGGAT ATCCTGAAAG GAGGAACTGA ACGATTCCTT 
ACCAACATTA AAGACACTTC CTCTAAAGTC ATCCAGTCGG TGGCCAGCTC CAGTTATGCT 
AAAGGAGACC TGGACCTGTC CTACATCACC TCCAGACTAG CAGTCATGTC TTTCCCAGCT 
GAAGGTGTTG AATCTGCTAT TAAGAATAAC ATTGAGGATG TGCGTCTGTT CCTGGACTCT 
CGACACGCGG GTCATTACGC CGTTTATAAC CTGTCCAAAC GCTCCTACAG ACCAGCCAGG 
TTTCACAACA GGGTATCCGA GTGCGGTTGG CAGCCAAGGC GAGCTCCTAC ACTGAAGAAC 
TTGTACAGTA TATGTAAAAA CATGCATCAG TGGCTCAAAC AGGACCAGAG GAACATCTGC 
ATCGTACACT GTCTGGACGG ACGGGCAGCG TCTGCTGTGG TGGTTTGTGC CTTCCTCTGT 
TTCTGTCGTC TCTTCTCCAC CGCTGAGGCT GCAGTTTACA TGTTCAGCAT GAAGCGATGT 
CCACCGGGCA TCTCAGCCTC ACACAAAAGG TATATTGAAT ACATGTGTGA TATGATGGCA 
GAAGAGCCCA TAATCCCCCA CAGCAAGCCT ATAACCATTC GTTCAGTAGC CATGACGCCC 
GTACCGCTCT TTAATAAGCA GAGGAATGGC TGTCGGCCGT TCTGTGAGGT CTATGTTGGA 
GATGAGCGTG TCGCCACCAC CTCACAGGAG TACGACAGAA TGAGGGATTT TAAGATGGAG 
GACGGGCGTG CGGAGATTCC TCTGAATATC ACCGTGCAGG GTGACGTTTT GATTGTGATT 
TATCATGCAC GATCCACGCT CGGCGGACGC CTGCAGGCCA AAATGGCATC CATGAAAATG 
TTTCAGATTC AGTTTCATAC AGGCTTTGTG CCCAGAAATG CCTCCACTGT CAAATTTGCA 
AAGTATGATC TGGACGCGTG TGACATCCAG GAGAAATATC CTGACCTCTT CCAGGTGCAC 
GTCAATGTGG AGGTGGAGCC TCAGGATCGT CCCAGCAACA AGACTCCACC CTGGGAGAAC 
TTCGCCACCA AAGGACTCAA TCCCAAGATC CTCTTCTCCA GCCGAGATGA ACAGCAGGAG 
ATTCTGACTA AATTTGGTAA ACCAGAGCTT CCTCGTCAGC CTGGTTCGAC AGCCTGTTAT 
GCCTCTGACC CACCGCAGTC GGAGAACTCT CCAGATGACC CGCAGACTGA GTCGGACTCA 
CCGTCACCCC AGAGCACAGA CACCAACGCC AACAACTTCT TTCAGACGCT GGACTGGGAA 
GACACAAGTC GACACTTTGA CCACGCTGAC GGCCACTTGG ATGACCAGGA AGATTTGAGC 
GGCGGCTCGG AGGAGGAGTC AGTGTCGTAC TCTGCCCCTA TTGAAGGGAC TCTGTCACGG 
GACACCAGTG AGCCGCTGTT TGAAGCAGAC TTTACAAACA CTCCGCCTGA AGCTCAACCC 
GACACTGAGG ACCTGCTGGG TCTGAACTCT GAGCCCGGTC CTGCTGTTCC AGAGAGCGGT 
TTGAAGAGCT CCGCCAGTAA CAGTAATTTA CTGAACGATC TACTGTTCGC CCCTCAGACC 
AACACTGTGC CTTCAGCGAG CAATGAAGAC CTGATGGGGG AAGATGCGGG AGAAGATCTC 
TTCTTTTCCA ACTCGTCCAG CCAGCCGCCA CCAGCCACTA ATAAAGATTT CTTCGACCCA 
TTCGGCGTGG GTTCGGCCTC CTCAGGGTCT GATTTGTTTG GTGATCTGCT GGGTTCAGAT 
GCTGCTAACA GTGGCTTCAA AGCTCCAGCT CCTGCATCAA ACTCCAGCCT CTTCAACCTC 
AACAAGTTAG CAAGTGAAAC CCCGAAGATG ACCTCATCAG CGAGTCAGCC TGATCTGTTG 
AGTGGGTGGG ACAGCTGGGC CACCAGCGGA ACCACCTCAG GAGTCTCGTC TTTCTCCAAG 
GCCAAATCCC AGAGTTTTGA CCCTTTCGCA GACCTGGGAA ATCTCGGCTC AACACTACCT 
GGCTCCTCCA GTGCTAGCTT TTCTGGCTCA GGGTTTAAAA CGGCGCCCTC TGCAGGCGGC 
CCTAAAAACT CAGGTCAGCA GTGGCAGCAA ACACCTCGAC CCTCTTCAGG CCCAAACAAA 
CCCTGGATGC CTCCGAACTC CACGGCCCGG CCACAAGCTG TTCCTCAAGC AGCCAAACCA 
AACTACAACC CCAGCTTTAG TGTGATCGGC GGACGGCAGG AGAGGGGCAT CAGGGGGCCC 
GGATTTGGTC CAAAGCCTAA AGTGAAAGAA GACGACTTTG AGGATCTTCT GTGCACACAA 
GGTTTTGGCT CCAAACCTGA CAGGAAGATG CCCAGAACAA TCGCTGAGAT GAGGAAGCAG 
GAGCTGTCCA AAGACATGGA CCCTCTCAAA CTACAGATCT TGGACTGGAT CGAGGGGAAG 
GAGAGGAACA TCCGCGCTCT TCTGTCCACG CTTCACACAG TCCTGTGGGA GGGCGAGACC 
CGCTGGAGAC CCATCAACAT GGCAGATCTG GTCACTCCAG ATCAGGTCAA GAGGGTCTAT 
AGGAAGGCTG TGCTCGTCGT GCATCCAGAC AAGGCCACGG GTCAGCCGTA CGAGCAGTAC 
GCTAAGATGA TCTTCATGGA GCTCAGCGAC GCCTGGTCCG AGTTCGAGAA CCAAGGATCC 
AAAGCCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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gak cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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