Gcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcn1
Entrez Gene ID	690632
Full Name	GCN1 eIF2 alpha kinase activator-like 1
General protein information	Preferred Names GCN1 eIF2 alpha kinase activator-like 1 Names eIF-2-alpha kinase activator GCN1 GCN1 general control of amino-acid synthesis 1-like 1 translational activator GCN1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001168664.1	NP_001162135.1	eIF-2-alpha kinase activator GCN1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GCN1L1	XP_534701.2
Danio rerio (zebrafish)	gcn1l1	XP_002667084.2
Caenorhabditis elegans (roundworm)	gcn-1	NP_497506.3
Oryza sativa (rice)	Os03g0721200	NP_001051102.1
Saccharomyces cerevisiae (baker's yeast)	GCN1	NP_011320.3
Arabidopsis thaliana (thale cress)	ILA	NP_176659.6
Schizosaccharomyces pombe (fission yeast)	SPAC18G6.05c	NP_593669.1
Magnaporthe oryzae (rice blast fungus)	MGG_04710	XP_003710832.1
Eremothecium gossypii	AGOS_AFR169W	NP_985716.2
Pan troglodytes (chimpanzee)	GCN1L1	XP_001159608.2
Mus musculus (house mouse)	Gcn1l1	NP_766307.2
Rattus norvegicus (Norway rat)	Gcn1l1	NP_001162135.1
Homo sapiens (human)	GCN1L1	NP_006827.1
Bos taurus (cattle)	GCN1L1	XP_002694568.1
Gallus gallus (chicken)	GCN1L1	XP_003642271.2
Neurospora crassa	NCU05803	XP_960063.1
Drosophila melanogaster (fruit fly)	CG17514	NP_001015316.1
Xenopus tropicalis (tropical clawed frog)	gcn1l1	NP_001096529.1
Kluyveromyces lactis	KLLA0F12430g	XP_455642.1
Macaca mulatta (Rhesus monkey)	GCN1L1	XP_001087027.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001700	XP_321391.4

Gene Ontology
Bibliography

Related articles in PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Cambiaghi TD, Pereira CM, Shanmugam R, Bolech M, Wek RC, Sattlegger E, Castilho BA
Biochemical and biophysical research communications443(2)592-7(2014 Jan)

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2α kinase GCN2 in the modulation of neurite outgrowth.
Roffé M, Hajj GN, Azevedo HF, Alves VS, Castilho BA
The Journal of biological chemistry288(15)10860-9(2013 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa15169	NM_001168664.1 Latest version!	Rattus norvegicus GCN1 activator of EIF2AK4 (Gcn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcn1 ( NM_001168664.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001162135.1 Rattus norvegicus GCN1 activator of EIF2AK4 (Gcn1), mRNA.

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CloneID	ORa15169
Clone ID Related Accession (Same CDS sequence)	NM_001168664.1
Accession Version	NM_001168664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555257600000
Organism	Rattus norvegicus(Norway rat)
Product	eIF-2-alpha kinase activator GCN1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123425.5. On or before Dec 13, 2009 this sequence version replaced XM_001072108.1, XM_001072034.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG ACACGCAGGT CTCCGAGACA CTGAAGCGCT TTGCAGTGAA GGTGACAACA 
GCTAGTGTGA AGGAGCGGAG AGAGATTCTC AGTGAACTGG GGCGATGTAT TGCTGGGAAA 
GATCTGCCAG AGGGAGCAGT GAAAGGGCTC TGCAAGCTGT TCTGCTTGAC CCTGCATCGA 
TACAGGGATG CAGCTTCTCG CAGAGCCCTG CAGGCAGCCA TTCAGCAGCT GGCTGAGGCC 
CAGCCAGAAG CCACAGCCAA GAACCTCCTG CACTCTCTTC AGTCCTCAGG GGTCGGCTCC 
AAAGCGTGTG TTCCCAGCAA AAGCAGCGGC TCAGCCGCCT TGCTGGCCCT GACCTGGACC 
TGCCTTCTTG TGCGGATTGT CTTTCCCCTG AAGGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAGCTGGTGG AGGTGCAGTG TCTGCTGCTG CTGGAGGTGC TGGGCGGCTC CCACAAGCAT 
GCTGTGGACG GCGCTGTGAA GAAACTCACT AAGCTATGGA AAGAGAACCC TGGGCTGGTG 
GAGCAGTACT TCTCAGCCAT CCTTAGCCTA GAACCCAACC AAAACTATGC CGGCATGCTG 
GGGCTGCTCG TGCAGTTCTG TACAAATCAC AAAGAGATGG ACGTGGTCAA TCAGCACAAG 
AGTACCCTGC TGGAGTTCTA CGTGAAGCAC ATTCTGATGA GCAAAGCCAA GCCTCCCAAG 
TACTTGTTGG ACAACTGCGC CCCGCTGCTC CGGTTCATGT CCCACTCTGA GTTTAAGGAC 
CTGATTCTGC CTACCATACA GAAGTCCTTG CTGAGGAGCC CGGAGAATGT TATAGAGACT 
ATCTCCAGCC TGCTGGCTTC AGTGACACTT GATCTCAGCC AGTATGCCCT GGACATCGTG 
AAAGGCTTGG CAAATCAGCT GAAGTCCAAC AGCCCCCGCC TGATGGACGA AGCGGTCCTG 
GCACTGCGTA ACCTGTCCCG TCAGTGCAGT GACTCGTCCG CCACCGAGGC CCTGACTAAG 
CATCTGTTTG CAATCCTTGG AGGCTCCGAA GGGAAGCTGA CCATCATAGC CCAGAAGATG 
AGCGTCCTTT CAGGGATTGG AAGCCTCAGT CATCATGGGG TATCGGGGCC TTCAGGTCAA 
GTTCTGAATG GGTGTGTGGC TGAGCTGTTC ATCCCGTTCC TGCAACAGGA AGTGCACGAA 
GGGACCTTGG TACATGCTGT CTCTGTCCTG GCGCTCTGGT GCAACCGGTT CACGACAGAA 
GTGCCCAAGA AGCTCACTGA CTGGTTCAAG AAAGTTTTCA GCCTTAAAAC CTCCACTTCT 
GCAGTGAGGC ACGCCTACCT GCAGTGCATG CTGGCCTCCT TCCGAGGTGA CACACTCTTG 
CAGGCCTTGG ACTTTCTGCC CTTGCTCATG CAGACAGTAG AGAAGGCGGC CTCCCAGAGC 
ACTCAGGTCC CCACAGTCAC CGAAGGCGTC GCCGCAGCCC TGTTGGTCTC CAAGCTCTCT 
GTGGCCGACT CACAAGCTGA GGCCAAACTG AGCAACTTTT GGCAGCTGGT TGTGGATGAG 
AAGAAGCAGA TTTTCACATC TGAGAAGTTT CTGCTCCTGG CTTCAGAGGA GGCCCTGTGT 
ACTGTGCTGC ATCTGACCGA GAGGCTTTTC CTTGACCACG CCCACAGGCT CACCAACAGC 
AGAGTACAGC AGTACTACCG GGTCCTGGTG GCTGTGCTCT TGAGCCGCAC CTGGCATGTG 
CGCAGGCAGG CCCAGCAGAC AGTCCGGAAG CTGCTGTCCT CTCTTGGGGG CATTAAGCTA 
GCCAACGGCC TTTTGGAGGA GCTGAAGACT GTCCTCAACT CTCACAAGGT GCTACCCTTG 
GAGACTCTTG TGACAGATGC TGGGGAGGTA ATGGAGACAG GCAAGACCTA TGTGCCTCCT 
CGAGTTCTGC AGGAAGCTCT GTGTGTCATC TCCGGCGTTC CGGGGCTCAA GGGCGACATC 
ACCAGCACCG AGCAGCTGGC CCAGGAGATG CTGATCATCT CCCACCACCC GTCCTTAGTG 
GCGGTGCAGT CTGGACTCTG GCCAGCACTT CTCACCAGGA TGAAGATTGA CCCTGAAGCC 
TTTATCACCA GGCACCTGGA CCAGATCATC CCCAGGATCA CTACACAGAG CCCCCTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTT TCCATCCTGT CTCCTGATCG AGTCCTCCCT 
CAGCTCATCA GCAGTATCAC CGCGTCTGTG CAGAATCCTG CGCTGTGCCT CGTGACTCGG 
GAGGAGTTTG CTGTCATGCA GACCCCTGCC GGAGAGCTGT TTGATAAATC CATCATCCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA GGCCTACTCT 
TTTAAGGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAAA TAAAGAAGAA GAAAGGCATA 
AAAGAGGAGG TTCAGCTGAC GAGTAAGCAG AAGGAGATGC TGCAGGCCCA GATGGACAAG 
GAGGCGCAAA TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AACTAGAAGC AGCGCTTGGA 
CTCCTAGACG CCATTATGAC CAGGAACCCA CGTGGCCTGA CACAGTACAT CCCTGTTCTG 
GTTGACGCCT TCCTGCCCTT GCTGAAGTCT CCTCTGGCTG CTCCCAGGGT CAAAGGCCCC 
TTCCTGTCTT TGGCTGCCTG TGTCATGCCC CCTAGACTCA AGACCTTGGG CACCTTGGTG 
AGTCATGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGCTC TGGATCAGTC CTGGTGCCAA 
GAAGAGCTGC CGGTGGCTGT CAGGAGGGCT GTGTCACTGC TGCACACACA CACCATTCCC 
AGCAGGGTGG GCAAGGGCGA GCCAGATGCT GCACCGCTGT CTGCACCCGC CTTCTCCTTG 
ATCTTCCCGA TGTTAAAGAT GGTGCTCACT GAGATGCCCT ACCACAGCGA GGAGGAAGAG 
GAGCAGATGG CCCAGATCCT TCAGATCCTC ACTGTCCACG CTCAGCTGAG GGCTTCCCCT 
GACACCCCGC CCGAGCGGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG AGTGGCCATG 
CTACGTCTGC TGACGTGGGT GATCGGAACG GGCTCCCCTC GCCTACAGGT TCTTGCCTCA 
GACACTCTGA CCGCCCTATG TGCCAGCAGC AGTGGGGAGG ACGGCTGTGC CTTTGCGGAG 
CAGGAGGAGG TGGATGTGCT GCTCGCTGCC TTGCAGTCCC CATGTGCCAG CGTGCGCGAG 
ACTGCGCTCC GGGGGCTGAT GGAGCTCCAC CTGGTGTTGC CAGCCCCTGA CACTGATGAG 
AAGAACGGCC TGAACCTGCT GCGGAGACTC TGGGTTGTGA AGTTTGACAA GGAGGAGGAG 
ATACGGAAGC TGGCTGAGAG GCTTTGGACG ACCATGGGCC TCGATCTGCA GTCAGATCTC 
TGCTCGTTGC TGATCGATGA TGTGATCTAT CACGAGGCTG CTGTGAGGCA GGCTGGGGCT 
GAAGCCCTTT CGCAGGCAGT GGCACGATAC CAGCGGCAAG CAGCAGAGGT TATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCACCCC CAGTGCTGGA TGCTTTGGGC 
CGAGTGATTT CAGAATCCCC TCCAGACCAG TGGGAAGCCA GGTGTGGCCT GGCCTTGGCC 
CTGAACAAGC TCTCACAGTA TTTGGATAGC TCTCAGGTGA AGCCGCTCTT TCAGTTTTTT 
GTCCCGGATG CCCTCAATGA CCGAAACCCA GATGTGCGGA AGTGCATGTT AGATGCAGCG 
CTTGCCACAC TCAATGCCCA TGGGAAGGAG AATGTCAACT CACTGCTGCC CGTGTTTGAG 
GAGTTCCTGA AGGACGCGCC CAATGATGCC AGCTACGACG CCGTGCGACA GAGTGTGGTG 
GTTCTGATGG GCTCTTTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAACCCATT 
GTTGCCAAGC TCATTGCCGC CCTCTCCACA CCTTCTCAGC AGGTCCAGGA GTCAGTCGCC 
AGCTGCTTAC CGCCACTTGT GCCTGCCGTC AAGGAGGACG CTGGGGGCAT GATTCAGCGG 
CTCATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAGGGGGC CGCGTACGGG 
CTGGCGGGCC TGGTGAAAGG CCTGGGCATC CTTTCCCTGA AGCAGCAGGA AATGATGGCT 
GCACTCACCG ATGCTATCCA GGACAAGAAG AACTTCCGAA GGCGAGAGGG AGCTCTGTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTCG AGCCCTATGT GGTCCACGTG 
CTGCCCCATC TATTGCTGTG CTTTGGAGAT GGCAATCAGT ACGTACGCGA GGCTGCAGAT 
GACTGTGCCA AAGCTGTGAT GAGCAATTTG AGTGCTCATG GTGTGAAGTT GGTGCTCCCG 
TCCCTGTTAG CTGCCCTGGA GGAGGAGTCC TGGAGGACCA AAGCTGGGTC AGTAGAGCTG 
CTCGGGGCGA TGGCGTACTG TGCACCTAAG CAGCTGTCCT CCTGCCTGCC CAACATTGTG 
CCCAAGCTGA CAGAGGTGCT GACAGACTCC CACGTCAAGG TGCAGAAGGC TGGGCAGCAA 
GCCCTCAGGC AGATCGGCTC TGTCATCAGG AACCCGGAGA TTCTCGCCAT TGCCCCGGTC 
CTGCTGGACG CTCTGACGGA CCCATCGAGG AAGACTCAGA AGTGCCTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGACGCC CCGTCCCTGG CACTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGATCG CTCCACAGAC ACACGCAAGA TGGCCGCGCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCCAG TGTGACACCT 
GGCCTGAAAG CCTCACTCTT AGACCCTGTC CCTGAGGTGC GCACAGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTGAAGGG CATGGGAGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACTC TGACATATGA GCAGAGCTCT GTGGACCGCT CAGGCGCTGC CCAAGGGTTG 
GCTGAGGTAA TGGCTGGCTT AGGAGTAGAG AAGTTGGAAA AATTGATGCC AGAAATTGTG 
GCCACTGCCA GCAAAGTGGA CATCGCACCC CATGTCCGAG ACGGCTACAT CATGATGTTC 
AACTACCTGC CTATCACCTT TGGGGACAAG TTTACCCCTT ATGTGGGGCC CATCATCCCT 
TGTATCCTCA AAGCTCTGGC TGATGAGAAT GAGTTCGTTC GTGACACCGC CCTCCGTGCC 
GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACAGCCATTG CCCTGCTGCT GCCCCAGCTG 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATCCGGTTCA GCTCTGTTCA ACTCCTTGGG 
GACCTCCTGT TCCACATCTC TGGGGTCACT GGGAAAATGA CCACAGAAAC AGCCTCTGAG 
GATGATAACT TTGGAACTGC TCAGTCTAAC AAGGCAATCA TCACGGCCCT GGGGGTCGAT 
CGGCGGAACC GGGTCCTGGC AGGGCTCTAT ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
AGGCAGGCGT CACTGCACGT GTGGAAGATT GTCGTTTCCA ACACTCCCCG CACCCTGCGA 
GAGATCCTGC CCACTCTCTT TGGTCTGCTG CTGGGTTTCC TGGCTAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCCAG GACGTTGGGA GATCTTGTCC GGAAGCTGGG GGAGAAGATC 
CTCCCTGAGA TCATCCCCAT CCTCGAAGAA GGGCTAAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGCGTGT GCATCGGGCT CAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCTGTGCTC 
TATTTCTCTG AGTCCCTTGT GCCCACGGCA CGGAAGGCAC TATGTGATCC CCTGGAGGAG 
GTCCGGGAGG CGGCGGCCAA AACCTTTGAG CAGCTGCACT CCACCATTGG CCACCAGGCT 
TTGGAGGACA TTCTCCCGTT CCTGCTGAAG CAGCTGGATG ACGAGGAGGT GTCAGAATTC 
GCCCTGGATG GTCTGAAGCA GGTCATGGCC GTTAAGAGCC GTGTGGTGCT GCCCTATCTG 
GTACCCAAGC TGACGACACC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT CTCATCTGTG 
GCTGGCGATG CTCTGACGCG TCACCTTGGT GTGATTCTCC CAGCAGTCAT GTTAGCGCTG 
AAGGAAAAGC TTGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCTGTCATC 
CTCTCGGTAG AGGATGACAC TGGGCACCGG ATCATCATTG AGGATCTGCT GGAGGCCACT 
CGAAGCCCTG AGGTGGGCAT GAGGCAGGCC GCCGCCATCA TCCTCAACAT GTACTGCTCC 
CGCTCCAAGG CTGACTATAC CAGCCACCTG CGCAGCCTGG TCTCCGGCTT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGGG GAGAGCTGGG ATGCTCTCAA CGCCATCACT 
AAGAAGCTGG ATGCCGGCAA CCAGCTGGCC CTGATCGAAG AATTCCATAA GGAAATCCGC 
TACATAGGCA ACGAGTGCCG AGGGGAGCAT GTGCCGGGCT TCTGCCTTCC AAAGAAGGGA 
GTAACCTCCA TCCTTCCAGT GCTACGGGAA GGAGTCCTCA CAGGCAGTCC TGAGCAAAAG 
GAAGAAGCAG CCAAGGCCTT GGGCTTGGTG ATCCGCCTGA CGTCGGCCGA TGCCCTGAGG 
CCCTCTGTGG TCAGCATCAC TGGCCCTCTG ATTCGCATCC TTGGGGACCG GTTCAGCTGG 
AGTGTGAAGG CGGCCCTGCT GGAGACACTC AGCCTCTTGC TGGGCAAGGT CGGGATCGCC 
CTGAAGCCCT TCCTGCCCCA GCTTCAGACC ACCTTCACCA AAGCTCTGCA GGACTCCAAT 
CGGGGCGTAC GCCTCAAGGC TGCGGATGCC CTAGGGAAAC TCATTTCCAT CCACATCAAG 
GTGGACCCCC TTTTTACAGA GCTGCTCAAT GGAATCCGTG TGGTGGAGGA CCCAGGTATA 
AGGGACACCA TGTTGCAGGC CCTCAGGTTT GTGATTCAGG GTGCGGGCGC CAAAGTGGAT 
GCTGCCATCC GGAAAAACAT TGTCTCACTC CTGCTGGGCA TGCTGGGCCA CGACGAGGAC 
AACACTCGGA TCTCTTCAGC CGGGTGCCTT GGGGAGCTGT GTGCCTTTCT GACTGAGGAG 
GAGCTCAACA CGGTCCTGCA GCAGTGCTTG CTGGCTGACG TATCTGGCAT TGACTGGATG 
GTTCGGCACG GCCGGAGCTT GGCGCTGTCT GTGGCTGTGA ACGTGGCTCC CAGCAGACTC 
TGCACAGGCA AATACAGCAA TGAGGTTCAG GACATGGTCC TCAGCAACGC TGTGGCAGAC 
AGGATCCCCA TTGCCGTGAG TGGGATTCGG GGCATGGGCT TCCTCATGAA GTATCACATC 
GAGACAGGAG GGGGACAGCT GCCACCCAGA CTCTCCACCC TGCTCATTAA GTGTCTGCAG 
AACCCGTCCA GTGACATCAG GCTGGTTGCC GAGAAGATGA TCTGGTGGGC CAACAAGGAG 
CCCCGGCCTG CCCTGGAGCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT ACTCGACAAC 
ACCAAGGACA AGAACACGGT AGTGAGGGCC TACAGCGAGC AGGCCATCGT GAATCTGCTC 
AAACTGAGGC AGGGCGAGGA GCTGCTGCAG TCACTCTCCA AGATCCTGGA TGTGGCCAGC 
TTGGAGGCGC TGAATGAGTG CAGCCGGCGG TCGCTGAAGA AGCTGGCCTG CCAGGCGGAC 
TCTGTGGAGC AGGTGGATGA CACCATCCTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162135.1
CDS	1..8016
Translation	MAADTQVSETLKRFAVKVTTASVKERREILSELGRCIAGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGVGSKACVPSKSSGSAALLALTWTCLLVRIVFPLKAKRQGDIWNKLVEVQCLLLLEVLGGSHKHAVDGAVKKLTKLWKENPGLVEQYFSAILSLEPNQNYAGMLGLLVQFCTNHKEMDVVNQHKSTLLEFYVKHILMSKAKPPKYLLDNCAPLLRFMSHSEFKDLILPTIQKSLLRSPENVIETISSLLASVTLDLSQYALDIVKGLANQLKSNSPRLMDEAVLALRNLSRQCSDSSATEALTKHLFAILGGSEGKLTIIAQKMSVLSGIGSLSHHGVSGPSGQVLNGCVAELFIPFLQQEVHEGTLVHAVSVLALWCNRFTTEVPKKLTDWFKKVFSLKTSTSAVRHAYLQCMLASFRGDTLLQALDFLPLLMQTVEKAASQSTQVPTVTEGVAAALLVSKLSVADSQAEAKLSNFWQLVVDEKKQIFTSEKFLLLASEEALCTVLHLTERLFLDHAHRLTNSRVQQYYRVLVAVLLSRTWHVRRQAQQTVRKLLSSLGGIKLANGLLEELKTVLNSHKVLPLETLVTDAGEVMETGKTYVPPRVLQEALCVISGVPGLKGDITSTEQLAQEMLIISHHPSLVAVQSGLWPALLTRMKIDPEAFITRHLDQIIPRITTQSPLNQSSMNAMGSLSILSPDRVLPQLISSITASVQNPALCLVTREEFAVMQTPAGELFDKSIIQSAQQDSIKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKEEVQLTSKQKEMLQAQMDKEAQIRRRLQELDGELEAALGLLDAIMTRNPRGLTQYIPVLVDAFLPLLKSPLAAPRVKGPFLSLAACVMPPRLKTLGTLVSHVTLRLLKPECALDQSWCQEELPVAVRRAVSLLHTHTIPSRVGKGEPDAAPLSAPAFSLIFPMLKMVLTEMPYHSEEEEEQMAQILQILTVHAQLRASPDTPPERVDENGPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTALCASSSGEDGCAFAEQEEVDVLLAALQSPCASVRETALRGLMELHLVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAERLWTTMGLDLQSDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVPDALNDRNPDVRKCMLDAALATLNAHGKENVNSLLPVFEEFLKDAPNDASYDAVRQSVVVLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAVKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVDRRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAVKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNMYCSRSKADYTSHLRSLVSGLIRLFNDSSPVVLGESWDALNAITKKLDAGNQLALIEEFHKEIRYIGNECRGEHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALRPSVVSITGPLIRILGDRFSWSVKAALLETLSLLLGKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISIHIKVDPLFTELLNGIRVVEDPGIRDTMLQALRFVIQGAGAKVDAAIRKNIVSLLLGMLGHDEDNTRISSAGCLGELCAFLTEEELNTVLQQCLLADVSGIDWMVRHGRSLALSVAVNVAPSRLCTGKYSNEVQDMVLSNAVADRIPIAVSGIRGMGFLMKYHIETGGGQLPPRLSTLLIKCLQNPSSDIRLVAEKMIWWANKEPRPALEPQAIKPILKALLDNTKDKNTVVRAYSEQAIVNLLKLRQGEELLQSLSKILDVASLEALNECSRRSLKKLACQADSVEQVDDTILT

Target ORF information:

RefSeq Version	NM_001168664.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GCN1 activator of EIF2AK4 (Gcn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168664.1

ORF Insert Sequence:

ATGGCGGCGG ACACGCAGGT CTCCGAGACA CTGAAGCGCT TTGCAGTGAA GGTGACAACA 
GCTAGTGTGA AGGAGCGGAG AGAGATTCTC AGTGAACTGG GGCGATGTAT TGCTGGGAAA 
GATCTGCCAG AGGGAGCAGT GAAAGGGCTC TGCAAGCTGT TCTGCTTGAC CCTGCATCGA 
TACAGGGATG CAGCTTCTCG CAGAGCCCTG CAGGCAGCCA TTCAGCAGCT GGCTGAGGCC 
CAGCCAGAAG CCACAGCCAA GAACCTCCTG CACTCTCTTC AGTCCTCAGG GGTCGGCTCC 
AAAGCGTGTG TTCCCAGCAA AAGCAGCGGC TCAGCCGCCT TGCTGGCCCT GACCTGGACC 
TGCCTTCTTG TGCGGATTGT CTTTCCCCTG AAGGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAGCTGGTGG AGGTGCAGTG TCTGCTGCTG CTGGAGGTGC TGGGCGGCTC CCACAAGCAT 
GCTGTGGACG GCGCTGTGAA GAAACTCACT AAGCTATGGA AAGAGAACCC TGGGCTGGTG 
GAGCAGTACT TCTCAGCCAT CCTTAGCCTA GAACCCAACC AAAACTATGC CGGCATGCTG 
GGGCTGCTCG TGCAGTTCTG TACAAATCAC AAAGAGATGG ACGTGGTCAA TCAGCACAAG 
AGTACCCTGC TGGAGTTCTA CGTGAAGCAC ATTCTGATGA GCAAAGCCAA GCCTCCCAAG 
TACTTGTTGG ACAACTGCGC CCCGCTGCTC CGGTTCATGT CCCACTCTGA GTTTAAGGAC 
CTGATTCTGC CTACCATACA GAAGTCCTTG CTGAGGAGCC CGGAGAATGT TATAGAGACT 
ATCTCCAGCC TGCTGGCTTC AGTGACACTT GATCTCAGCC AGTATGCCCT GGACATCGTG 
AAAGGCTTGG CAAATCAGCT GAAGTCCAAC AGCCCCCGCC TGATGGACGA AGCGGTCCTG 
GCACTGCGTA ACCTGTCCCG TCAGTGCAGT GACTCGTCCG CCACCGAGGC CCTGACTAAG 
CATCTGTTTG CAATCCTTGG AGGCTCCGAA GGGAAGCTGA CCATCATAGC CCAGAAGATG 
AGCGTCCTTT CAGGGATTGG AAGCCTCAGT CATCATGGGG TATCGGGGCC TTCAGGTCAA 
GTTCTGAATG GGTGTGTGGC TGAGCTGTTC ATCCCGTTCC TGCAACAGGA AGTGCACGAA 
GGGACCTTGG TACATGCTGT CTCTGTCCTG GCGCTCTGGT GCAACCGGTT CACGACAGAA 
GTGCCCAAGA AGCTCACTGA CTGGTTCAAG AAAGTTTTCA GCCTTAAAAC CTCCACTTCT 
GCAGTGAGGC ACGCCTACCT GCAGTGCATG CTGGCCTCCT TCCGAGGTGA CACACTCTTG 
CAGGCCTTGG ACTTTCTGCC CTTGCTCATG CAGACAGTAG AGAAGGCGGC CTCCCAGAGC 
ACTCAGGTCC CCACAGTCAC CGAAGGCGTC GCCGCAGCCC TGTTGGTCTC CAAGCTCTCT 
GTGGCCGACT CACAAGCTGA GGCCAAACTG AGCAACTTTT GGCAGCTGGT TGTGGATGAG 
AAGAAGCAGA TTTTCACATC TGAGAAGTTT CTGCTCCTGG CTTCAGAGGA GGCCCTGTGT 
ACTGTGCTGC ATCTGACCGA GAGGCTTTTC CTTGACCACG CCCACAGGCT CACCAACAGC 
AGAGTACAGC AGTACTACCG GGTCCTGGTG GCTGTGCTCT TGAGCCGCAC CTGGCATGTG 
CGCAGGCAGG CCCAGCAGAC AGTCCGGAAG CTGCTGTCCT CTCTTGGGGG CATTAAGCTA 
GCCAACGGCC TTTTGGAGGA GCTGAAGACT GTCCTCAACT CTCACAAGGT GCTACCCTTG 
GAGACTCTTG TGACAGATGC TGGGGAGGTA ATGGAGACAG GCAAGACCTA TGTGCCTCCT 
CGAGTTCTGC AGGAAGCTCT GTGTGTCATC TCCGGCGTTC CGGGGCTCAA GGGCGACATC 
ACCAGCACCG AGCAGCTGGC CCAGGAGATG CTGATCATCT CCCACCACCC GTCCTTAGTG 
GCGGTGCAGT CTGGACTCTG GCCAGCACTT CTCACCAGGA TGAAGATTGA CCCTGAAGCC 
TTTATCACCA GGCACCTGGA CCAGATCATC CCCAGGATCA CTACACAGAG CCCCCTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTT TCCATCCTGT CTCCTGATCG AGTCCTCCCT 
CAGCTCATCA GCAGTATCAC CGCGTCTGTG CAGAATCCTG CGCTGTGCCT CGTGACTCGG 
GAGGAGTTTG CTGTCATGCA GACCCCTGCC GGAGAGCTGT TTGATAAATC CATCATCCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA GGCCTACTCT 
TTTAAGGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAAA TAAAGAAGAA GAAAGGCATA 
AAAGAGGAGG TTCAGCTGAC GAGTAAGCAG AAGGAGATGC TGCAGGCCCA GATGGACAAG 
GAGGCGCAAA TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AACTAGAAGC AGCGCTTGGA 
CTCCTAGACG CCATTATGAC CAGGAACCCA CGTGGCCTGA CACAGTACAT CCCTGTTCTG 
GTTGACGCCT TCCTGCCCTT GCTGAAGTCT CCTCTGGCTG CTCCCAGGGT CAAAGGCCCC 
TTCCTGTCTT TGGCTGCCTG TGTCATGCCC CCTAGACTCA AGACCTTGGG CACCTTGGTG 
AGTCATGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGCTC TGGATCAGTC CTGGTGCCAA 
GAAGAGCTGC CGGTGGCTGT CAGGAGGGCT GTGTCACTGC TGCACACACA CACCATTCCC 
AGCAGGGTGG GCAAGGGCGA GCCAGATGCT GCACCGCTGT CTGCACCCGC CTTCTCCTTG 
ATCTTCCCGA TGTTAAAGAT GGTGCTCACT GAGATGCCCT ACCACAGCGA GGAGGAAGAG 
GAGCAGATGG CCCAGATCCT TCAGATCCTC ACTGTCCACG CTCAGCTGAG GGCTTCCCCT 
GACACCCCGC CCGAGCGGGT GGATGAGAAT GGCCCGGAGT TGCTGCCCCG AGTGGCCATG 
CTACGTCTGC TGACGTGGGT GATCGGAACG GGCTCCCCTC GCCTACAGGT TCTTGCCTCA 
GACACTCTGA CCGCCCTATG TGCCAGCAGC AGTGGGGAGG ACGGCTGTGC CTTTGCGGAG 
CAGGAGGAGG TGGATGTGCT GCTCGCTGCC TTGCAGTCCC CATGTGCCAG CGTGCGCGAG 
ACTGCGCTCC GGGGGCTGAT GGAGCTCCAC CTGGTGTTGC CAGCCCCTGA CACTGATGAG 
AAGAACGGCC TGAACCTGCT GCGGAGACTC TGGGTTGTGA AGTTTGACAA GGAGGAGGAG 
ATACGGAAGC TGGCTGAGAG GCTTTGGACG ACCATGGGCC TCGATCTGCA GTCAGATCTC 
TGCTCGTTGC TGATCGATGA TGTGATCTAT CACGAGGCTG CTGTGAGGCA GGCTGGGGCT 
GAAGCCCTTT CGCAGGCAGT GGCACGATAC CAGCGGCAAG CAGCAGAGGT TATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCACCCC CAGTGCTGGA TGCTTTGGGC 
CGAGTGATTT CAGAATCCCC TCCAGACCAG TGGGAAGCCA GGTGTGGCCT GGCCTTGGCC 
CTGAACAAGC TCTCACAGTA TTTGGATAGC TCTCAGGTGA AGCCGCTCTT TCAGTTTTTT 
GTCCCGGATG CCCTCAATGA CCGAAACCCA GATGTGCGGA AGTGCATGTT AGATGCAGCG 
CTTGCCACAC TCAATGCCCA TGGGAAGGAG AATGTCAACT CACTGCTGCC CGTGTTTGAG 
GAGTTCCTGA AGGACGCGCC CAATGATGCC AGCTACGACG CCGTGCGACA GAGTGTGGTG 
GTTCTGATGG GCTCTTTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAACCCATT 
GTTGCCAAGC TCATTGCCGC CCTCTCCACA CCTTCTCAGC AGGTCCAGGA GTCAGTCGCC 
AGCTGCTTAC CGCCACTTGT GCCTGCCGTC AAGGAGGACG CTGGGGGCAT GATTCAGCGG 
CTCATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAGGGGGC CGCGTACGGG 
CTGGCGGGCC TGGTGAAAGG CCTGGGCATC CTTTCCCTGA AGCAGCAGGA AATGATGGCT 
GCACTCACCG ATGCTATCCA GGACAAGAAG AACTTCCGAA GGCGAGAGGG AGCTCTGTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTCG AGCCCTATGT GGTCCACGTG 
CTGCCCCATC TATTGCTGTG CTTTGGAGAT GGCAATCAGT ACGTACGCGA GGCTGCAGAT 
GACTGTGCCA AAGCTGTGAT GAGCAATTTG AGTGCTCATG GTGTGAAGTT GGTGCTCCCG 
TCCCTGTTAG CTGCCCTGGA GGAGGAGTCC TGGAGGACCA AAGCTGGGTC AGTAGAGCTG 
CTCGGGGCGA TGGCGTACTG TGCACCTAAG CAGCTGTCCT CCTGCCTGCC CAACATTGTG 
CCCAAGCTGA CAGAGGTGCT GACAGACTCC CACGTCAAGG TGCAGAAGGC TGGGCAGCAA 
GCCCTCAGGC AGATCGGCTC TGTCATCAGG AACCCGGAGA TTCTCGCCAT TGCCCCGGTC 
CTGCTGGACG CTCTGACGGA CCCATCGAGG AAGACTCAGA AGTGCCTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGACGCC CCGTCCCTGG CACTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGATCG CTCCACAGAC ACACGCAAGA TGGCCGCGCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCCAG TGTGACACCT 
GGCCTGAAAG CCTCACTCTT AGACCCTGTC CCTGAGGTGC GCACAGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTGAAGGG CATGGGAGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACTC TGACATATGA GCAGAGCTCT GTGGACCGCT CAGGCGCTGC CCAAGGGTTG 
GCTGAGGTAA TGGCTGGCTT AGGAGTAGAG AAGTTGGAAA AATTGATGCC AGAAATTGTG 
GCCACTGCCA GCAAAGTGGA CATCGCACCC CATGTCCGAG ACGGCTACAT CATGATGTTC 
AACTACCTGC CTATCACCTT TGGGGACAAG TTTACCCCTT ATGTGGGGCC CATCATCCCT 
TGTATCCTCA AAGCTCTGGC TGATGAGAAT GAGTTCGTTC GTGACACCGC CCTCCGTGCC 
GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACAGCCATTG CCCTGCTGCT GCCCCAGCTG 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATCCGGTTCA GCTCTGTTCA ACTCCTTGGG 
GACCTCCTGT TCCACATCTC TGGGGTCACT GGGAAAATGA CCACAGAAAC AGCCTCTGAG 
GATGATAACT TTGGAACTGC TCAGTCTAAC AAGGCAATCA TCACGGCCCT GGGGGTCGAT 
CGGCGGAACC GGGTCCTGGC AGGGCTCTAT ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
AGGCAGGCGT CACTGCACGT GTGGAAGATT GTCGTTTCCA ACACTCCCCG CACCCTGCGA 
GAGATCCTGC CCACTCTCTT TGGTCTGCTG CTGGGTTTCC TGGCTAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCCAG GACGTTGGGA GATCTTGTCC GGAAGCTGGG GGAGAAGATC 
CTCCCTGAGA TCATCCCCAT CCTCGAAGAA GGGCTAAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGCGTGT GCATCGGGCT CAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCTGTGCTC 
TATTTCTCTG AGTCCCTTGT GCCCACGGCA CGGAAGGCAC TATGTGATCC CCTGGAGGAG 
GTCCGGGAGG CGGCGGCCAA AACCTTTGAG CAGCTGCACT CCACCATTGG CCACCAGGCT 
TTGGAGGACA TTCTCCCGTT CCTGCTGAAG CAGCTGGATG ACGAGGAGGT GTCAGAATTC 
GCCCTGGATG GTCTGAAGCA GGTCATGGCC GTTAAGAGCC GTGTGGTGCT GCCCTATCTG 
GTACCCAAGC TGACGACACC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT CTCATCTGTG 
GCTGGCGATG CTCTGACGCG TCACCTTGGT GTGATTCTCC CAGCAGTCAT GTTAGCGCTG 
AAGGAAAAGC TTGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCTGTCATC 
CTCTCGGTAG AGGATGACAC TGGGCACCGG ATCATCATTG AGGATCTGCT GGAGGCCACT 
CGAAGCCCTG AGGTGGGCAT GAGGCAGGCC GCCGCCATCA TCCTCAACAT GTACTGCTCC 
CGCTCCAAGG CTGACTATAC CAGCCACCTG CGCAGCCTGG TCTCCGGCTT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGGG GAGAGCTGGG ATGCTCTCAA CGCCATCACT 
AAGAAGCTGG ATGCCGGCAA CCAGCTGGCC CTGATCGAAG AATTCCATAA GGAAATCCGC 
TACATAGGCA ACGAGTGCCG AGGGGAGCAT GTGCCGGGCT TCTGCCTTCC AAAGAAGGGA 
GTAACCTCCA TCCTTCCAGT GCTACGGGAA GGAGTCCTCA CAGGCAGTCC TGAGCAAAAG 
GAAGAAGCAG CCAAGGCCTT GGGCTTGGTG ATCCGCCTGA CGTCGGCCGA TGCCCTGAGG 
CCCTCTGTGG TCAGCATCAC TGGCCCTCTG ATTCGCATCC TTGGGGACCG GTTCAGCTGG 
AGTGTGAAGG CGGCCCTGCT GGAGACACTC AGCCTCTTGC TGGGCAAGGT CGGGATCGCC 
CTGAAGCCCT TCCTGCCCCA GCTTCAGACC ACCTTCACCA AAGCTCTGCA GGACTCCAAT 
CGGGGCGTAC GCCTCAAGGC TGCGGATGCC CTAGGGAAAC TCATTTCCAT CCACATCAAG 
GTGGACCCCC TTTTTACAGA GCTGCTCAAT GGAATCCGTG TGGTGGAGGA CCCAGGTATA 
AGGGACACCA TGTTGCAGGC CCTCAGGTTT GTGATTCAGG GTGCGGGCGC CAAAGTGGAT 
GCTGCCATCC GGAAAAACAT TGTCTCACTC CTGCTGGGCA TGCTGGGCCA CGACGAGGAC 
AACACTCGGA TCTCTTCAGC CGGGTGCCTT GGGGAGCTGT GTGCCTTTCT GACTGAGGAG 
GAGCTCAACA CGGTCCTGCA GCAGTGCTTG CTGGCTGACG TATCTGGCAT TGACTGGATG 
GTTCGGCACG GCCGGAGCTT GGCGCTGTCT GTGGCTGTGA ACGTGGCTCC CAGCAGACTC 
TGCACAGGCA AATACAGCAA TGAGGTTCAG GACATGGTCC TCAGCAACGC TGTGGCAGAC 
AGGATCCCCA TTGCCGTGAG TGGGATTCGG GGCATGGGCT TCCTCATGAA GTATCACATC 
GAGACAGGAG GGGGACAGCT GCCACCCAGA CTCTCCACCC TGCTCATTAA GTGTCTGCAG 
AACCCGTCCA GTGACATCAG GCTGGTTGCC GAGAAGATGA TCTGGTGGGC CAACAAGGAG 
CCCCGGCCTG CCCTGGAGCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT ACTCGACAAC 
ACCAAGGACA AGAACACGGT AGTGAGGGCC TACAGCGAGC AGGCCATCGT GAATCTGCTC 
AAACTGAGGC AGGGCGAGGA GCTGCTGCAG TCACTCTCCA AGATCCTGGA TGTGGCCAGC 
TTGGAGGCGC TGAATGAGTG CAGCCGGCGG TCGCTGAAGA AGCTGGCCTG CCAGGCGGAC 
TCTGTGGAGC AGGTGGATGA CACCATCCTG ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.
Biochemical and biophysical research communications443(2)592-7(2014 Jan)
Cambiaghi TD,Pereira CM,Shanmugam R,Bolech M,Wek RC,Sattlegger E,Castilho BA

IMPACT is a developmentally regulated protein in neurons that opposes the eukaryotic initiation factor 2α kinase GCN2 in the modulation of neurite outgrowth.
The Journal of biological chemistry288(15)10860-9(2013 Apr)
Roffé M,Hajj GN,Azevedo HF,Alves VS,Castilho BA

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Gcn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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