GCN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN1
Entrez Gene ID	10985
Full Name	GCN1, eIF2 alpha kinase activator homolog
Synonyms	GCN1L,GCN1L1,PRIC295
General protein information	Preferred Names GCN1, eIF2 alpha kinase activator homolog Names eIF-2-alpha kinase activator GCN1 GCN1 (general control of amino-acid synthesis 1, yeast)-like 1 GCN1 eIF-2-alpha kinase activator homolog GCN1 general control of amino-acid synthesis 1-like 1 GCN1-like protein 1 general control of amino-acid synthesis 1-like protein 1 hsGCN1 peroxisome proliferator activated receptor interacting complex protein translational activator GCN1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.23
Disorder MIM:	605614

mRNA and Protein(s)

mRNA	Protein	Name
NM_006836.1	NP_006827.1	eIF-2-alpha kinase activator GCN1
NM_006836.2	NP_006827.1	eIF-2-alpha kinase activator GCN1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GCN1L1	XP_534701.2
Danio rerio (zebrafish)	gcn1l1	XP_002667084.2
Caenorhabditis elegans (roundworm)	gcn-1	NP_497506.3
Oryza sativa (rice)	Os03g0721200	NP_001051102.1
Saccharomyces cerevisiae (baker's yeast)	GCN1	NP_011320.3
Arabidopsis thaliana (thale cress)	ILA	NP_176659.6
Schizosaccharomyces pombe (fission yeast)	SPAC18G6.05c	NP_593669.1
Magnaporthe oryzae (rice blast fungus)	MGG_04710	XP_003710832.1
Eremothecium gossypii	AGOS_AFR169W	NP_985716.2
Pan troglodytes (chimpanzee)	GCN1L1	XP_001159608.2
Mus musculus (house mouse)	Gcn1l1	NP_766307.2
Rattus norvegicus (Norway rat)	Gcn1l1	NP_001162135.1
Homo sapiens (human)	GCN1L1	NP_006827.1
Bos taurus (cattle)	GCN1L1	XP_002694568.1
Gallus gallus (chicken)	GCN1L1	XP_003642271.2
Neurospora crassa	NCU05803	XP_960063.1
Drosophila melanogaster (fruit fly)	CG17514	NP_001015316.1
Xenopus tropicalis (tropical clawed frog)	gcn1l1	NP_001096529.1
Kluyveromyces lactis	KLLA0F12430g	XP_455642.1
Macaca mulatta (Rhesus monkey)	GCN1L1	XP_001087027.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001700	XP_321391.4

Gene Ontology
Bibliography

Related articles in PubMed

Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response.
Silva RC, Sattlegger E, Castilho BA
Journal of cell science129(24)4521-4533(2016 12)

Interactome analysis of herpes simplex virus 1 envelope glycoprotein H.
Hirohata Y, Kato A, Oyama M, Kozuka-Hata H, Koyanagi N, Arii J, Kawaguchi Y
Microbiology and immunology59(6)331-7(2015 Jun)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05723	NM_006836.2 Latest version!	Homo sapiens GCN1 activator of EIF2AK4 (GCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu05723	NM_006836.1	Homo sapiens GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN1 ( NM_006836.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_006827.1 Homo sapiens GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.

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CloneID	OHu05723
Clone ID Related Accession (Same CDS sequence)	NM_006836.1 , NM_006836.2
Accession Version	NM_006836.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase activator GCN1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D86973.2, U88837.1, BQ929459.1, BU186578.1 and BC021129.1. On Oct 25, 2004 this sequence version replaced XM_045792.11. ##Evidence-Data-START## Transcript exon combination :: BC153881.1, BK006575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACAACA 
GCCAGTGTAA AGGAACGGAG AGAAATCCTC AGTGAACTTG GGAAGTGTGT TGCTGGAAAA 
GATCTTCCAG AGGGAGCAGT GAAGGGGCTC TGCAAATTGT TCTGCTTGAC TCTGCATCGA 
TATAGAGATG CAGCCTCCCG CAGGGCCTTG CAGGCAGCCA TCCAGCAGTT GGCTGAGGCC 
CAGCCAGAAG CCACTGCTAA GAACCTTCTA CACTCTCTGC AGTCTTCTGG TATAGGCTCC 
AAAGCAGGTG TTCCCAGTAA GAGCAGTGGC TCTGCCGCCT TGCTGGCCTT GACCTGGACC 
TGCCTCCTGG TGCGCATTGT CTTTCCATCG AGAGCCAAGC GACAAGGAGA CATCTGGAAC 
AAACTGGTGG AAGTGCAGTG CCTGCTCTTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGATG GTGCTGTGAA GAAACTCACG AAGCTGTGGA AAGAGAACCC CGGGCTGGTG 
GAACAGTACT TGTCAGCCAT TCTCAGCCTA GAGCCCAACC AGAACTATGC TGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGTCAC AAGGAGATGG ACGTGGTCAG TCAGCACAAG 
AGCGCCCTAC TGGACTTTTA CATGAAGAAC ATCCTGATGA GCAAAGTCAA GCCTCCGAAG 
TACCTGTTGG ATAGCTGTGC CCCTCTGCTC CGATACCTGT CCCACTCAGA ATTTAAGGAT 
CTGATACTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT TATTGAAACT 
ATTTCTAGTC TGCTGGCATC AGTGACGCTT GACCTCAGCC AGTATGCCAT GGACATCGTG 
AAAGGACTGG CTGGTCACCT GAAATCCAAC AGTCCCCGCC TGATGGATGA AGCTGTGCTG 
GCACTGCGGA ACCTGGCACG CCAGTGCAGT GACTCTTCGG CCATGGAATC CCTGACCAAG 
CACCTATTTG CTATCCTCGG AGGCTCGGAA GGAAAACTAA CTGTTGTAGC CCAGAAGATG 
AGCGTCCTCT CAGGGATTGG GAGCGTCAGT CATCACGTGG TGTCTGGACC TTCCAGTCAG 
GTCCTGAATG GGATCGTGGC TGAGCTGTTC ATCCCGTTCC TTCAGCAGGA AGTTCATGAA 
GGGACCTTGG TACACGCTGT CTCAGTCCTG GCTCTCTGGT GTAACCGATT CACTATGGAA 
GTGCCCAAGA AGCTCACTGA ATGGTTCAAA AAAGCTTTCA GCCTTAAAAC CTCCACATCT 
GCGGTGAGGC ATGCCTACCT GCAGTGCATG TTGGCCTCTT ACCGGGGTGA CACGCTGTTG 
CAGGCCCTGG ACTTACTGCC CTTGCTCATC CAGACAGTGG AGAAGGCAGC CTCCCAAAGC 
ACTCAGGTTC CCACCATCAC TGAAGGGGTT GCCGCAGCCT TGTTGCTCTT AAAGTTGTCA 
GTGGCTGACT CACAGGCTGA GGCCAAACTG AGCAGTTTCT GGCAGTTGAT TGTGGATGAG 
AAAAAGCAGG TTTTCACTTC TGAGAAATTC CTGGTCATGG CTTCAGAGGA TGCCCTGTGT 
ACTGTGTTGC ATCTGACAGA GAGACTTTTC CTTGACCACC CGCATAGACT CACTGGCAAC 
AAAGTTCAGC AGTACCACCG GGCTCTGGTG GCGGTGCTCC TGAGCCGCAC CTGGCACGTC 
CGCAGGCAGG CTCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG CTTTAAGCTG 
GCGCACGGAC TCTTGGAGGA GCTGAAGACT GTCCTCAGTT CTCACAAGGT GCTGCCCTTA 
GAGGCTTTGG TGACTGATGC TGGAGAGGTG ACTGAGGCAG GCAAGGCCTA CGTGCCTCCA 
CGGGTCCTGC AGGAGGCTCT GTGTGTCATC TCCGGTGTGC CAGGGCTCAA GGGTGATGTC 
ACCGACACTG AACAACTGGC CCAGGAAATG CTGATCATCT CCCACCACCC ATCCTTAGTT 
GCCGTGCAGT CTGGACTTTG GCCAGCACTT CTTGCCAGGA TGAAGATCGA TCCTGAAGCC 
TTTATCACCA GGCACCTGGA TCAGATCATT CCCAGGATGA CCACACAGAG TCCCCTAAAC 
CAGTCCTCCA TGAATGCCAT GGGCTCCCTT TCCGTCCTGT CGCCGGACCG GGTCCTCCCA 
CAGCTCATCA GCACCATCAC TGCCTCCGTG CAGAACCCTG CACTGCGCCT GGTGACGCGG 
GAGGAGTTTG CCATTATGCA GACCCCTGCT GGGGAGCTGT ATGACAAATC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTATTCC 
TTCAAAGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATC 
AAAGAGGAGG TGCAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAGGCCCA GCTAGACAGG 
GAGGCGCAGG TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AGCTGGAGGC GGCGCTTGGA 
CTGCTGGACA TCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCTGTTTTG 
GTCGACTCTT TTCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGAACCCC 
TTCTTGTCCT TGGCTGCCTG TGTCATGCCC TCTAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCACGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGTCC TGGATAAGTC CTGGTGCCAG 
GAAGAGCTGT CGGTGGCTGT GAAGAGGGCG GTGATGCTGC TGCACACCCA CACCATCACC 
AGCAGGGTGG GCAAGGGGGA GCCAGGTGCT GCGCCCTTGT CCGCGCCAGC CTTCTCCTTA 
GTCTTCCCGT TTCTGAAGAT GGTGCTGACG GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAGTGGATGG CCCAGATTCT TCAGATCCTC ACTGTCCAAG CCCAGCTGAG GGCCTCCCCC 
AACACCCCAC CCGGGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGTCTTC TGACTTGGGT GATCGGGACG GGCTCGCCTC GCTTACAGGT TCTGGCTTCA 
GACACCCTGA CCACCCTGTG TGCCAGCAGC AGTGGTGATG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGTGCC TTGCAGTCCC CGTGTGCCAG CGTGCGGGAA 
ACCGTGCTCC GGGGGCTGAT GGAACTCCAC ATGGTATTGC CAGCACCTGA TACTGATGAG 
AAGAATGGCC TGAACCTTCT GCGGAGACTC TGGGTGGTCA AGTTTGACAA GGAGGAGGAG 
ATCCGGAAGC TGGCTGAGAG GCTCTGGTCA ATGATGGGCC TAGACCTGCA GCCAGACCTC 
TGCTCCTTGC TGATTGACGA CGTGATCTAT CATGAGGCGG CTGTAAGGCA GGCAGGGGCC 
GAAGCCCTCT CCCAAGCAGT GGCACGTTAC CAGCGGCAGG CGGCGGAGGT TATGGGCAGG 
CTCATGGAGA TTTACCAGGA AAAGCTCTAC CGGCCGCCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTTATTT CAGAATCTCC TCCAGATCAG TGGGAAGCCA GGTGTGGCTT GGCGTTGGCC 
CTCAACAAGC TCTCCCAGTA TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTCCCTGATG CCCTCAATGA CCGACACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCC 
CTCGCAACGC TCAACACTCA TGGGAAGGAG AACGTCAACT CGCTGTTGCC AGTATTCGAG 
GAGTTCCTGA AGAACGCGCC CAATGATGCC AGCTATGATG CTGTGCGACA GAGTGTGGTG 
GTCCTGATGG GCTCTCTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCCATT 
GTTGCCAAGC TCATCGCTGC CCTCTCCACC CCCTCCCAGC AGGTCCAGGA GTCCGTAGCC 
AGCTGCTTGC CACCCCTTGT GCCAGCCATC AAGGAGGATG CTGGAGGGAT GATCCAGAGG 
CTTATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAAGGGGC CGCCTATGGC 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTCTCGCTGA AGCAACAGGA GATGATGGCG 
GCACTGACTG ATGCCATCCA AGATAAGAAG AACTTCCGCC GGCGAGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAACTTTTTG AGCCGTATGT GGTTCACGTG 
CTGCCCCATC TGCTCCTGTG CTTTGGGGAT GGAAACCAGT ATGTGCGTGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACTTG AGTGCTCACG GGGTGAAGCT GGTGCTCCCC 
TCCTTACTGG CTGCCCTGGA GGAGGAATCG TGGCGGACCA AAGCTGGGTC AGTGGAGCTT 
CTTGGGGCAA TGGCGTACTG TGCTCCTAAG CAGCTGTCAT CCTGTCTACC CAACATTGTG 
CCCAAGCTTA CGGAGGTGCT GACCGACTCC CATGTCAAAG TCCAGAAGGC TGGACAGCAG 
GCGCTCAGGC AGATCGGCTC CGTTATCAGG AACCCGGAGA TCCTGGCCAT TGCTCCAGTC 
CTCCTGGATG CCCTGACGGA TCCCTCCAGG AAGACCCAGA AGTGCTTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGATGCC CCATCCCTGG CCCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACGCGGAAGA TGGCAGCCCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCGT ACCTGCCCAG CGTGACGCCT 
GGCCTGAAAG CATCGCTTTT GGACCCTGTG CCTGAGGTGC GGACCGTATC TGCAAAGGCC 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTATGA GCAGAGCTCT GTGGATCGCT CAGGCGCTGC ACAGGGGTTG 
GCTGAGGTCA TGGCCGGTTT GGGGGTGGAG AAGTTGGAGA AGTTGATGCC AGAAATCGTG 
GCTACAGCCA GCAAAGTGGA CATTGCACCC CATGTCCGAG ATGGCTACAT TATGATGTTT 
AACTACCTGC CCATCACCTT TGGAGACAAG TTTACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAT GAGTTTGTGC GTGACACCGC CCTGCGCGCG 
GGCCAGCGGG TTATCTCCAT GTACGCTGAG ACAGCCATCG CCCTGCTGCT GCCCCAGCTA 
GAGCAAGGCC TCTTTGATGA CCTTTGGAGA ATCAGGTTCA GCTCTGTTCA GCTCCTTGGG 
GATCTCCTGT TTCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCTGAG 
GATGATAACT TTGGAACTGC CCAGTCCAAC AAGGCGATCA TCACTGCCCT GGGGGTAGAG 
CGGCGGAACC GGGTGTTGGC AGGGCTGTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
CGGCAGGCGT CCCTGCATGT CTGGAAGATT GTTGTCTCCA ATACCCCCCG CACCTTGCGT 
GAGATCCTAC CCACTCTCTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCGAG AACATTGGGA GATCTTGTGC GGAAGTTAGG GGAGAAAATC 
CTCCCCGAGA TCATCCCCAT CCTTGAGGAA GGCCTGAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGTGTGT GCATTGGCCT AAGTGAGATC ATGAAGTCCA CCAGCCGGGA TGCCGTGCTG 
TATTTCTCTG AATCCCTCGT GCCCACGGCA AGGAAGGCTT TGTGTGACCC ACTGGAGGAG 
GTCAGAGAGG CGGCAGCCAA GACTTTCGAG CAGCTGCATT CCACCATCGG CCACCAGGCT 
CTGGAGGACA TTCTCCCATT TTTACTAAAG CAGCTGGATG ACGAGGAGGT GTCAGAGTTT 
GCCTTGGATG GTCTGAAGCA AGTCATGGCT ATTAAGAGTC GTGTGGTGCT GCCCTACCTT 
GTGCCCAAGC TGACAACGCC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT TTCGTCAGTG 
GCTGGTGATG CCCTCACCCG TCATCTTGGC GTGATCCTCC CAGCGGTCAT GCTGGCCCTG 
AAGGAAAAGC TTGGGACCCC AGATGAGCAG CTGGAGATGG CCAATTGTCA GGCTGTGATC 
CTCTCCGTAG AGGATGACAC AGGGCACCGG ATCATCATCG AGGATCTGCT GGAGGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAAGCT GCTGCCATCA TCCTCAACAT CTACTGTTCC 
CGCTCAAAGG CTGACTACAC CAGCCACCTG CGGAGCCTGG TCTCGGGCCT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ATGCCCTAAA TGCCATCACT 
AAGAAGCTGG ATGCTGGCAA CCAGTTGGCA CTCATTGAAG AGCTGCACAA GGAAATCCGG 
CTCATAGGGA ACGAGAGCAA AGGCGAGCAT GTGCCAGGAT TCTGCCTCCC GAAGAAGGGA 
GTGACCTCCA TCCTTCCAGT GTTGCGGGAA GGAGTCCTGA CTGGCAGCCC TGAGCAGAAG 
GAGGAGGCAG CCAAAGCCTT AGGCTTGGTA ATCCGCCTGA CCTCGGCTGA CGCCCTGAGG 
CCCTCCGTGG TCAGCATCAC TGGCCCTCTG ATCCGCATCC TGGGGGACAG GTTCAGCTGG 
AATGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGT TGGCTAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACTTTCACCA AAGCCCTGCA GGACTCCAAC 
CGGGGGGTGC GCCTGAAGGC CGCAGATGCT CTGGGGAAGC TCATTTCCAT CCACATTAAG 
GTGGACCCCC TCTTCACAGA GCTGCTCAAT GGCATCCGCG CCATGGAGGA CCCAGGTGTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCAGGGGC CAAAGTGGAT 
GCCGTCATCC GGAAAAACAT CGTCTCACTC CTGCTGAGCA TGCTGGGACA CGATGAGGAC 
AACACTCGCA TCTCCTCAGC CGGGTGCCTA GGGGAACTGT GTGCCTTTTT GACTGAAGAG 
GAGCTTAGTG CCGTTCTACA GCAGTGCTTG CTGGCGGACG TGTCCGGCAT TGACTGGATG 
GTTCGGCACG GGCGGAGCCT GGCACTTTCC GTGGCTGTGA ATGTGGCTCC TGGCAGACTT 
TGTGCCGGCA GATATAGCAG TGATGTTCAG GAAATGATCC TGAGCAGTGC CACGGCGGAC 
AGGATCCCCA TTGCGGTGAG CGGGGTCCGG GGCATGGGCT TTCTCATGAG ACACCACATC 
GAGACAGGCG GAGGGCAGTT GCCGGCCAAA CTTTCCAGCC TGTTCGTTAA GTGTCTGCAG 
AACCCATCCA GCGACATCAG GCTGGTGGCT GAGAAGATGA TCTGGTGGGC AAATAAGGAC 
CCACTGCCTC CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT TCTTGACAAC 
ACCAAGGATA AGAACACCGT GGTCAGGGCC TACAGCGACC AGGCAATTGT CAACCTCCTC 
AAGATGCGGC AGGGTGAAGA GGTGTTTCAG TCCCTCTCCA AGATCCTGGA TGTGGCCAGT 
TTGGAGGTGC TGAACGAGGT TAACCGAAGG TCCCTGAAGA AGCTGGCCAG CCAGGCCGAC 
TCCACGGAGC AGGTGGACGA CACCATCCTG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006827.1
CDS	14..8029
Translation	MAADTQVSETLKRFAGKVTTASVKERREILSELGKCVAGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGIGSKAGVPSKSSGSAALLALTWTCLLVRIVFPSRAKRQGDIWNKLVEVQCLLLLEVLGGSHKHAVDGAVKKLTKLWKENPGLVEQYLSAILSLEPNQNYAGMLGLLVQFCTSHKEMDVVSQHKSALLDFYMKNILMSKVKPPKYLLDSCAPLLRYLSHSEFKDLILPTIQKSLLRSPENVIETISSLLASVTLDLSQYAMDIVKGLAGHLKSNSPRLMDEAVLALRNLARQCSDSSAMESLTKHLFAILGGSEGKLTVVAQKMSVLSGIGSVSHHVVSGPSSQVLNGIVAELFIPFLQQEVHEGTLVHAVSVLALWCNRFTMEVPKKLTEWFKKAFSLKTSTSAVRHAYLQCMLASYRGDTLLQALDLLPLLIQTVEKAASQSTQVPTITEGVAAALLLLKLSVADSQAEAKLSSFWQLIVDEKKQVFTSEKFLVMASEDALCTVLHLTERLFLDHPHRLTGNKVQQYHRALVAVLLSRTWHVRRQAQQTVRKLLSSLGGFKLAHGLLEELKTVLSSHKVLPLEALVTDAGEVTEAGKAYVPPRVLQEALCVISGVPGLKGDVTDTEQLAQEMLIISHHPSLVAVQSGLWPALLARMKIDPEAFITRHLDQIIPRMTTQSPLNQSSMNAMGSLSVLSPDRVLPQLISTITASVQNPALRLVTREEFAIMQTPAGELYDKSIIQSAQQDSIKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKEEVQLTSKQKEMLQAQLDREAQVRRRLQELDGELEAALGLLDIILAKNPSGLTQYIPVLVDSFLPLLKSPLAAPRIKNPFLSLAACVMPSRLKALGTLVSHVTLRLLKPECVLDKSWCQEELSVAVKRAVMLLHTHTITSRVGKGEPGAAPLSAPAFSLVFPFLKMVLTEMPHHSEEEEEWMAQILQILTVQAQLRASPNTPPGRVDENGPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTTLCASSSGDDGCAFAEQEEVDVLLCALQSPCASVRETVLRGLMELHMVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAERLWSMMGLDLQPDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVPDALNDRHPDVRKCMLDAALATLNTHGKENVNSLLPVFEEFLKNAPNDASYDAVRQSVVVLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAIKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNIYCSRSKADYTSHLRSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGNQLALIEELHKEIRLIGNESKGEHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALRPSVVSITGPLIRILGDRFSWNVKAALLETLSLLLAKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISIHIKVDPLFTELLNGIRAMEDPGVRDTMLQALRFVIQGAGAKVDAVIRKNIVSLLLSMLGHDEDNTRISSAGCLGELCAFLTEEELSAVLQQCLLADVSGIDWMVRHGRSLALSVAVNVAPGRLCAGRYSSDVQEMILSSATADRIPIAVSGVRGMGFLMRHHIETGGGQLPAKLSSLFVKCLQNPSSDIRLVAEKMIWWANKDPLPPLDPQAIKPILKALLDNTKDKNTVVRAYSDQAIVNLLKMRQGEEVFQSLSKILDVASLEVLNEVNRRSLKKLASQADSTEQVDDTILT

Target ORF information:

RefSeq Version	NM_006836.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006836.1

ORF Insert Sequence:

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACAACA 
GCCAGTGTAA AGGAACGGAG AGAAATCCTC AGTGAACTTG GGAAGTGTGT TGCTGGAAAA 
GATCTTCCAG AGGGAGCAGT GAAGGGGCTC TGCAAATTGT TCTGCTTGAC TCTGCATCGA 
TATAGAGATG CAGCCTCCCG CAGGGCCTTG CAGGCAGCCA TCCAGCAGTT GGCTGAGGCC 
CAGCCAGAAG CCACTGCTAA GAACCTTCTA CACTCTCTGC AGTCTTCTGG TATAGGCTCC 
AAAGCAGGTG TTCCCAGTAA GAGCAGTGGC TCTGCCGCCT TGCTGGCCTT GACCTGGACC 
TGCCTCCTGG TGCGCATTGT CTTTCCATCG AGAGCCAAGC GACAAGGAGA CATCTGGAAC 
AAACTGGTGG AAGTGCAGTG CCTGCTCTTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGATG GTGCTGTGAA GAAACTCACG AAGCTGTGGA AAGAGAACCC CGGGCTGGTG 
GAACAGTACT TGTCAGCCAT TCTCAGCCTA GAGCCCAACC AGAACTATGC TGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGTCAC AAGGAGATGG ACGTGGTCAG TCAGCACAAG 
AGCGCCCTAC TGGACTTTTA CATGAAGAAC ATCCTGATGA GCAAAGTCAA GCCTCCGAAG 
TACCTGTTGG ATAGCTGTGC CCCTCTGCTC CGATACCTGT CCCACTCAGA ATTTAAGGAT 
CTGATACTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT TATTGAAACT 
ATTTCTAGTC TGCTGGCATC AGTGACGCTT GACCTCAGCC AGTATGCCAT GGACATCGTG 
AAAGGACTGG CTGGTCACCT GAAATCCAAC AGTCCCCGCC TGATGGATGA AGCTGTGCTG 
GCACTGCGGA ACCTGGCACG CCAGTGCAGT GACTCTTCGG CCATGGAATC CCTGACCAAG 
CACCTATTTG CTATCCTCGG AGGCTCGGAA GGAAAACTAA CTGTTGTAGC CCAGAAGATG 
AGCGTCCTCT CAGGGATTGG GAGCGTCAGT CATCACGTGG TGTCTGGACC TTCCAGTCAG 
GTCCTGAATG GGATCGTGGC TGAGCTGTTC ATCCCGTTCC TTCAGCAGGA AGTTCATGAA 
GGGACCTTGG TACACGCTGT CTCAGTCCTG GCTCTCTGGT GTAACCGATT CACTATGGAA 
GTGCCCAAGA AGCTCACTGA ATGGTTCAAA AAAGCTTTCA GCCTTAAAAC CTCCACATCT 
GCGGTGAGGC ATGCCTACCT GCAGTGCATG TTGGCCTCTT ACCGGGGTGA CACGCTGTTG 
CAGGCCCTGG ACTTACTGCC CTTGCTCATC CAGACAGTGG AGAAGGCAGC CTCCCAAAGC 
ACTCAGGTTC CCACCATCAC TGAAGGGGTT GCCGCAGCCT TGTTGCTCTT AAAGTTGTCA 
GTGGCTGACT CACAGGCTGA GGCCAAACTG AGCAGTTTCT GGCAGTTGAT TGTGGATGAG 
AAAAAGCAGG TTTTCACTTC TGAGAAATTC CTGGTCATGG CTTCAGAGGA TGCCCTGTGT 
ACTGTGTTGC ATCTGACAGA GAGACTTTTC CTTGACCACC CGCATAGACT CACTGGCAAC 
AAAGTTCAGC AGTACCACCG GGCTCTGGTG GCGGTGCTCC TGAGCCGCAC CTGGCACGTC 
CGCAGGCAGG CTCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG CTTTAAGCTG 
GCGCACGGAC TCTTGGAGGA GCTGAAGACT GTCCTCAGTT CTCACAAGGT GCTGCCCTTA 
GAGGCTTTGG TGACTGATGC TGGAGAGGTG ACTGAGGCAG GCAAGGCCTA CGTGCCTCCA 
CGGGTCCTGC AGGAGGCTCT GTGTGTCATC TCCGGTGTGC CAGGGCTCAA GGGTGATGTC 
ACCGACACTG AACAACTGGC CCAGGAAATG CTGATCATCT CCCACCACCC ATCCTTAGTT 
GCCGTGCAGT CTGGACTTTG GCCAGCACTT CTTGCCAGGA TGAAGATCGA TCCTGAAGCC 
TTTATCACCA GGCACCTGGA TCAGATCATT CCCAGGATGA CCACACAGAG TCCCCTAAAC 
CAGTCCTCCA TGAATGCCAT GGGCTCCCTT TCCGTCCTGT CGCCGGACCG GGTCCTCCCA 
CAGCTCATCA GCACCATCAC TGCCTCCGTG CAGAACCCTG CACTGCGCCT GGTGACGCGG 
GAGGAGTTTG CCATTATGCA GACCCCTGCT GGGGAGCTGT ATGACAAATC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTATTCC 
TTCAAAGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATC 
AAAGAGGAGG TGCAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAGGCCCA GCTAGACAGG 
GAGGCGCAGG TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AGCTGGAGGC GGCGCTTGGA 
CTGCTGGACA TCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCTGTTTTG 
GTCGACTCTT TTCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGAACCCC 
TTCTTGTCCT TGGCTGCCTG TGTCATGCCC TCTAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCACGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGTCC TGGATAAGTC CTGGTGCCAG 
GAAGAGCTGT CGGTGGCTGT GAAGAGGGCG GTGATGCTGC TGCACACCCA CACCATCACC 
AGCAGGGTGG GCAAGGGGGA GCCAGGTGCT GCGCCCTTGT CCGCGCCAGC CTTCTCCTTA 
GTCTTCCCGT TTCTGAAGAT GGTGCTGACG GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAGTGGATGG CCCAGATTCT TCAGATCCTC ACTGTCCAAG CCCAGCTGAG GGCCTCCCCC 
AACACCCCAC CCGGGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGTCTTC TGACTTGGGT GATCGGGACG GGCTCGCCTC GCTTACAGGT TCTGGCTTCA 
GACACCCTGA CCACCCTGTG TGCCAGCAGC AGTGGTGATG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGTGCC TTGCAGTCCC CGTGTGCCAG CGTGCGGGAA 
ACCGTGCTCC GGGGGCTGAT GGAACTCCAC ATGGTATTGC CAGCACCTGA TACTGATGAG 
AAGAATGGCC TGAACCTTCT GCGGAGACTC TGGGTGGTCA AGTTTGACAA GGAGGAGGAG 
ATCCGGAAGC TGGCTGAGAG GCTCTGGTCA ATGATGGGCC TAGACCTGCA GCCAGACCTC 
TGCTCCTTGC TGATTGACGA CGTGATCTAT CATGAGGCGG CTGTAAGGCA GGCAGGGGCC 
GAAGCCCTCT CCCAAGCAGT GGCACGTTAC CAGCGGCAGG CGGCGGAGGT TATGGGCAGG 
CTCATGGAGA TTTACCAGGA AAAGCTCTAC CGGCCGCCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTTATTT CAGAATCTCC TCCAGATCAG TGGGAAGCCA GGTGTGGCTT GGCGTTGGCC 
CTCAACAAGC TCTCCCAGTA TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTCCCTGATG CCCTCAATGA CCGACACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCC 
CTCGCAACGC TCAACACTCA TGGGAAGGAG AACGTCAACT CGCTGTTGCC AGTATTCGAG 
GAGTTCCTGA AGAACGCGCC CAATGATGCC AGCTATGATG CTGTGCGACA GAGTGTGGTG 
GTCCTGATGG GCTCTCTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCCATT 
GTTGCCAAGC TCATCGCTGC CCTCTCCACC CCCTCCCAGC AGGTCCAGGA GTCCGTAGCC 
AGCTGCTTGC CACCCCTTGT GCCAGCCATC AAGGAGGATG CTGGAGGGAT GATCCAGAGG 
CTTATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAAGGGGC CGCCTATGGC 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTCTCGCTGA AGCAACAGGA GATGATGGCG 
GCACTGACTG ATGCCATCCA AGATAAGAAG AACTTCCGCC GGCGAGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAACTTTTTG AGCCGTATGT GGTTCACGTG 
CTGCCCCATC TGCTCCTGTG CTTTGGGGAT GGAAACCAGT ATGTGCGTGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACTTG AGTGCTCACG GGGTGAAGCT GGTGCTCCCC 
TCCTTACTGG CTGCCCTGGA GGAGGAATCG TGGCGGACCA AAGCTGGGTC AGTGGAGCTT 
CTTGGGGCAA TGGCGTACTG TGCTCCTAAG CAGCTGTCAT CCTGTCTACC CAACATTGTG 
CCCAAGCTTA CGGAGGTGCT GACCGACTCC CATGTCAAAG TCCAGAAGGC TGGACAGCAG 
GCGCTCAGGC AGATCGGCTC CGTTATCAGG AACCCGGAGA TCCTGGCCAT TGCTCCAGTC 
CTCCTGGATG CCCTGACGGA TCCCTCCAGG AAGACCCAGA AGTGCTTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGATGCC CCATCCCTGG CCCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACGCGGAAGA TGGCAGCCCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCGT ACCTGCCCAG CGTGACGCCT 
GGCCTGAAAG CATCGCTTTT GGACCCTGTG CCTGAGGTGC GGACCGTATC TGCAAAGGCC 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTATGA GCAGAGCTCT GTGGATCGCT CAGGCGCTGC ACAGGGGTTG 
GCTGAGGTCA TGGCCGGTTT GGGGGTGGAG AAGTTGGAGA AGTTGATGCC AGAAATCGTG 
GCTACAGCCA GCAAAGTGGA CATTGCACCC CATGTCCGAG ATGGCTACAT TATGATGTTT 
AACTACCTGC CCATCACCTT TGGAGACAAG TTTACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAT GAGTTTGTGC GTGACACCGC CCTGCGCGCG 
GGCCAGCGGG TTATCTCCAT GTACGCTGAG ACAGCCATCG CCCTGCTGCT GCCCCAGCTA 
GAGCAAGGCC TCTTTGATGA CCTTTGGAGA ATCAGGTTCA GCTCTGTTCA GCTCCTTGGG 
GATCTCCTGT TTCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCTGAG 
GATGATAACT TTGGAACTGC CCAGTCCAAC AAGGCGATCA TCACTGCCCT GGGGGTAGAG 
CGGCGGAACC GGGTGTTGGC AGGGCTGTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
CGGCAGGCGT CCCTGCATGT CTGGAAGATT GTTGTCTCCA ATACCCCCCG CACCTTGCGT 
GAGATCCTAC CCACTCTCTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCGAG AACATTGGGA GATCTTGTGC GGAAGTTAGG GGAGAAAATC 
CTCCCCGAGA TCATCCCCAT CCTTGAGGAA GGCCTGAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGTGTGT GCATTGGCCT AAGTGAGATC ATGAAGTCCA CCAGCCGGGA TGCCGTGCTG 
TATTTCTCTG AATCCCTCGT GCCCACGGCA AGGAAGGCTT TGTGTGACCC ACTGGAGGAG 
GTCAGAGAGG CGGCAGCCAA GACTTTCGAG CAGCTGCATT CCACCATCGG CCACCAGGCT 
CTGGAGGACA TTCTCCCATT TTTACTAAAG CAGCTGGATG ACGAGGAGGT GTCAGAGTTT 
GCCTTGGATG GTCTGAAGCA AGTCATGGCT ATTAAGAGTC GTGTGGTGCT GCCCTACCTT 
GTGCCCAAGC TGACAACGCC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT TTCGTCAGTG 
GCTGGTGATG CCCTCACCCG TCATCTTGGC GTGATCCTCC CAGCGGTCAT GCTGGCCCTG 
AAGGAAAAGC TTGGGACCCC AGATGAGCAG CTGGAGATGG CCAATTGTCA GGCTGTGATC 
CTCTCCGTAG AGGATGACAC AGGGCACCGG ATCATCATCG AGGATCTGCT GGAGGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAAGCT GCTGCCATCA TCCTCAACAT CTACTGTTCC 
CGCTCAAAGG CTGACTACAC CAGCCACCTG CGGAGCCTGG TCTCGGGCCT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ATGCCCTAAA TGCCATCACT 
AAGAAGCTGG ATGCTGGCAA CCAGTTGGCA CTCATTGAAG AGCTGCACAA GGAAATCCGG 
CTCATAGGGA ACGAGAGCAA AGGCGAGCAT GTGCCAGGAT TCTGCCTCCC GAAGAAGGGA 
GTGACCTCCA TCCTTCCAGT GTTGCGGGAA GGAGTCCTGA CTGGCAGCCC TGAGCAGAAG 
GAGGAGGCAG CCAAAGCCTT AGGCTTGGTA ATCCGCCTGA CCTCGGCTGA CGCCCTGAGG 
CCCTCCGTGG TCAGCATCAC TGGCCCTCTG ATCCGCATCC TGGGGGACAG GTTCAGCTGG 
AATGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGT TGGCTAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACTTTCACCA AAGCCCTGCA GGACTCCAAC 
CGGGGGGTGC GCCTGAAGGC CGCAGATGCT CTGGGGAAGC TCATTTCCAT CCACATTAAG 
GTGGACCCCC TCTTCACAGA GCTGCTCAAT GGCATCCGCG CCATGGAGGA CCCAGGTGTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCAGGGGC CAAAGTGGAT 
GCCGTCATCC GGAAAAACAT CGTCTCACTC CTGCTGAGCA TGCTGGGACA CGATGAGGAC 
AACACTCGCA TCTCCTCAGC CGGGTGCCTA GGGGAACTGT GTGCCTTTTT GACTGAAGAG 
GAGCTTAGTG CCGTTCTACA GCAGTGCTTG CTGGCGGACG TGTCCGGCAT TGACTGGATG 
GTTCGGCACG GGCGGAGCCT GGCACTTTCC GTGGCTGTGA ATGTGGCTCC TGGCAGACTT 
TGTGCCGGCA GATATAGCAG TGATGTTCAG GAAATGATCC TGAGCAGTGC CACGGCGGAC 
AGGATCCCCA TTGCGGTGAG CGGGGTCCGG GGCATGGGCT TTCTCATGAG ACACCACATC 
GAGACAGGCG GAGGGCAGTT GCCGGCCAAA CTTTCCAGCC TGTTCGTTAA GTGTCTGCAG 
AACCCATCCA GCGACATCAG GCTGGTGGCT GAGAAGATGA TCTGGTGGGC AAATAAGGAC 
CCACTGCCTC CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT TCTTGACAAC 
ACCAAGGATA AGAACACCGT GGTCAGGGCC TACAGCGACC AGGCAATTGT CAACCTCCTC 
AAGATGCGGC AGGGTGAAGA GGTGTTTCAG TCCCTCTCCA AGATCCTGGA TGTGGCCAGT 
TTGGAGGTGC TGAACGAGGT TAACCGAAGG TCCCTGAAGA AGCTGGCCAG CCAGGCCGAC 
TCCACGGAGC AGGTGGACGA CACCATCCTG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCN1 ( NM_006836.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006827.1 Homo sapiens GCN1 activator of EIF2AK4 (GCN1), mRNA.

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CloneID	OHu05723
Clone ID Related Accession (Same CDS sequence)	NM_006836.1 , NM_006836.2
Accession Version	NM_006836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase activator GCN1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004263.1, D86973.2, U88837.1, BQ929459.1, BU186578.1, BC021129.1 and AC004812.1. On Nov 23, 2018 this sequence version replaced NM_006836.1. ##Evidence-Data-START## Transcript exon combination :: BC153881.1, BK006575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000300648.7/ ENSP00000300648.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACAACA 
GCCAGTGTAA AGGAACGGAG AGAAATCCTC AGTGAACTTG GGAAGTGTGT TGCTGGAAAA 
GATCTTCCAG AGGGAGCAGT GAAGGGGCTC TGCAAATTGT TCTGCTTGAC TCTGCATCGA 
TATAGAGATG CAGCCTCCCG CAGGGCCTTG CAGGCAGCCA TCCAGCAGTT GGCTGAGGCC 
CAGCCAGAAG CCACTGCTAA GAACCTTCTA CACTCTCTGC AGTCTTCTGG TATAGGCTCC 
AAAGCAGGTG TTCCCAGTAA GAGCAGTGGC TCTGCCGCCT TGCTGGCCTT GACCTGGACC 
TGCCTCCTGG TGCGCATTGT CTTTCCATCG AGAGCCAAGC GACAAGGAGA CATCTGGAAC 
AAACTGGTGG AAGTGCAGTG CCTGCTCTTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGATG GTGCTGTGAA GAAACTCACG AAGCTGTGGA AAGAGAACCC CGGGCTGGTG 
GAACAGTACT TGTCAGCCAT TCTCAGCCTA GAGCCCAACC AGAACTATGC TGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGTCAC AAGGAGATGG ACGTGGTCAG TCAGCACAAG 
AGCGCCCTAC TGGACTTTTA CATGAAGAAC ATCCTGATGA GCAAAGTCAA GCCTCCGAAG 
TACCTGTTGG ATAGCTGTGC CCCTCTGCTC CGATACCTGT CCCACTCAGA ATTTAAGGAT 
CTGATACTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT TATTGAAACT 
ATTTCTAGTC TGCTGGCATC AGTGACGCTT GACCTCAGCC AGTATGCCAT GGACATCGTG 
AAAGGACTGG CTGGTCACCT GAAATCCAAC AGTCCCCGCC TGATGGATGA AGCTGTGCTG 
GCACTGCGGA ACCTGGCACG CCAGTGCAGT GACTCTTCGG CCATGGAATC CCTGACCAAG 
CACCTATTTG CTATCCTCGG AGGCTCGGAA GGAAAACTAA CTGTTGTAGC CCAGAAGATG 
AGCGTCCTCT CAGGGATTGG GAGCGTCAGT CATCACGTGG TGTCTGGACC TTCCAGTCAG 
GTCCTGAATG GGATCGTGGC TGAGCTGTTC ATCCCGTTCC TTCAGCAGGA AGTTCATGAA 
GGGACCTTGG TACACGCTGT CTCAGTCCTG GCTCTCTGGT GTAACCGATT CACTATGGAA 
GTGCCCAAGA AGCTCACTGA ATGGTTCAAA AAAGCTTTCA GCCTTAAAAC CTCCACATCT 
GCGGTGAGGC ATGCCTACCT GCAGTGCATG TTGGCCTCTT ACCGGGGTGA CACGCTGTTG 
CAGGCCCTGG ACTTACTGCC CTTGCTCATC CAGACAGTGG AGAAGGCAGC CTCCCAAAGC 
ACTCAGGTTC CCACCATCAC TGAAGGGGTT GCCGCAGCCT TGTTGCTCTT AAAGTTGTCA 
GTGGCTGACT CACAGGCTGA GGCCAAACTG AGCAGTTTCT GGCAGTTGAT TGTGGATGAG 
AAAAAGCAGG TTTTCACTTC TGAGAAATTC CTGGTCATGG CTTCAGAGGA TGCCCTGTGT 
ACTGTGTTGC ATCTGACAGA GAGACTTTTC CTTGACCACC CGCATAGACT CACTGGCAAC 
AAAGTTCAGC AGTACCACCG GGCTCTGGTG GCGGTGCTCC TGAGCCGCAC CTGGCACGTC 
CGCAGGCAGG CTCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG CTTTAAGCTG 
GCGCACGGAC TCTTGGAGGA GCTGAAGACT GTCCTCAGTT CTCACAAGGT GCTGCCCTTA 
GAGGCTTTGG TGACTGATGC TGGAGAGGTG ACTGAGGCAG GCAAGGCCTA CGTGCCTCCA 
CGGGTCCTGC AGGAGGCTCT GTGTGTCATC TCCGGTGTGC CAGGGCTCAA GGGTGATGTC 
ACCGACACTG AACAACTGGC CCAGGAAATG CTGATCATCT CCCACCACCC ATCCTTAGTT 
GCCGTGCAGT CTGGACTTTG GCCAGCACTT CTTGCCAGGA TGAAGATCGA TCCTGAAGCC 
TTTATCACCA GGCACCTGGA TCAGATCATT CCCAGGATGA CCACACAGAG TCCCCTAAAC 
CAGTCCTCCA TGAATGCCAT GGGCTCCCTT TCCGTCCTGT CGCCGGACCG GGTCCTCCCA 
CAGCTCATCA GCACCATCAC TGCCTCCGTG CAGAACCCTG CACTGCGCCT GGTGACGCGG 
GAGGAGTTTG CCATTATGCA GACCCCTGCT GGGGAGCTGT ATGACAAATC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTATTCC 
TTCAAAGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATC 
AAAGAGGAGG TGCAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAGGCCCA GCTAGACAGG 
GAGGCGCAGG TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AGCTGGAGGC GGCGCTTGGA 
CTGCTGGACA TCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCTGTTTTG 
GTCGACTCTT TTCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGAACCCC 
TTCTTGTCCT TGGCTGCCTG TGTCATGCCC TCTAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCACGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGTCC TGGATAAGTC CTGGTGCCAG 
GAAGAGCTGT CGGTGGCTGT GAAGAGGGCG GTGATGCTGC TGCACACCCA CACCATCACC 
AGCAGGGTGG GCAAGGGGGA GCCAGGTGCT GCGCCCTTGT CCGCGCCAGC CTTCTCCTTA 
GTCTTCCCGT TTCTGAAGAT GGTGCTGACG GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAGTGGATGG CCCAGATTCT TCAGATCCTC ACTGTCCAAG CCCAGCTGAG GGCCTCCCCC 
AACACCCCAC CCGGGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGTCTTC TGACTTGGGT GATCGGGACG GGCTCGCCTC GCTTACAGGT TCTGGCTTCA 
GACACCCTGA CCACCCTGTG TGCCAGCAGC AGTGGTGATG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGTGCC TTGCAGTCCC CGTGTGCCAG CGTGCGGGAA 
ACCGTGCTCC GGGGGCTGAT GGAACTCCAC ATGGTATTGC CAGCACCTGA TACTGATGAG 
AAGAATGGCC TGAACCTTCT GCGGAGACTC TGGGTGGTCA AGTTTGACAA GGAGGAGGAG 
ATCCGGAAGC TGGCTGAGAG GCTCTGGTCA ATGATGGGCC TAGACCTGCA GCCAGACCTC 
TGCTCCTTGC TGATTGACGA CGTGATCTAT CATGAGGCGG CTGTAAGGCA GGCAGGGGCC 
GAAGCCCTCT CCCAAGCAGT GGCACGTTAC CAGCGGCAGG CGGCGGAGGT TATGGGCAGG 
CTCATGGAGA TTTACCAGGA AAAGCTCTAC CGGCCGCCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTTATTT CAGAATCTCC TCCAGATCAG TGGGAAGCCA GGTGTGGCTT GGCGTTGGCC 
CTCAACAAGC TCTCCCAGTA TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTCCCTGATG CCCTCAATGA CCGACACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCC 
CTCGCAACGC TCAACACTCA TGGGAAGGAG AACGTCAACT CGCTGTTGCC AGTATTCGAG 
GAGTTCCTGA AGAACGCGCC CAATGATGCC AGCTATGATG CTGTGCGACA GAGTGTGGTG 
GTCCTGATGG GCTCTCTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCCATT 
GTTGCCAAGC TCATCGCTGC CCTCTCCACC CCCTCCCAGC AGGTCCAGGA GTCCGTAGCC 
AGCTGCTTGC CACCCCTTGT GCCAGCCATC AAGGAGGATG CTGGAGGGAT GATCCAGAGG 
CTTATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAAGGGGC CGCCTATGGC 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTCTCGCTGA AGCAACAGGA GATGATGGCG 
GCACTGACTG ATGCCATCCA AGATAAGAAG AACTTCCGCC GGCGAGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAACTTTTTG AGCCGTATGT GGTTCACGTG 
CTGCCCCATC TGCTCCTGTG CTTTGGGGAT GGAAACCAGT ATGTGCGTGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACTTG AGTGCTCACG GGGTGAAGCT GGTGCTCCCC 
TCCTTACTGG CTGCCCTGGA GGAGGAATCG TGGCGGACCA AAGCTGGGTC AGTGGAGCTT 
CTTGGGGCAA TGGCGTACTG TGCTCCTAAG CAGCTGTCAT CCTGTCTACC CAACATTGTG 
CCCAAGCTTA CGGAGGTGCT GACCGACTCC CATGTCAAAG TCCAGAAGGC TGGACAGCAG 
GCGCTCAGGC AGATCGGCTC CGTTATCAGG AACCCGGAGA TCCTGGCCAT TGCTCCAGTC 
CTCCTGGATG CCCTGACGGA TCCCTCCAGG AAGACCCAGA AGTGCTTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGATGCC CCATCCCTGG CCCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACGCGGAAGA TGGCAGCCCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCGT ACCTGCCCAG CGTGACGCCT 
GGCCTGAAAG CATCGCTTTT GGACCCTGTG CCTGAGGTGC GGACCGTATC TGCAAAGGCC 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTATGA GCAGAGCTCT GTGGATCGCT CAGGCGCTGC ACAGGGGTTG 
GCTGAGGTCA TGGCCGGTTT GGGGGTGGAG AAGTTGGAGA AGTTGATGCC AGAAATCGTG 
GCTACAGCCA GCAAAGTGGA CATTGCACCC CATGTCCGAG ATGGCTACAT TATGATGTTT 
AACTACCTGC CCATCACCTT TGGAGACAAG TTTACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAT GAGTTTGTGC GTGACACCGC CCTGCGCGCG 
GGCCAGCGGG TTATCTCCAT GTACGCTGAG ACAGCCATCG CCCTGCTGCT GCCCCAGCTA 
GAGCAAGGCC TCTTTGATGA CCTTTGGAGA ATCAGGTTCA GCTCTGTTCA GCTCCTTGGG 
GATCTCCTGT TTCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCTGAG 
GATGATAACT TTGGAACTGC CCAGTCCAAC AAGGCGATCA TCACTGCCCT GGGGGTAGAG 
CGGCGGAACC GGGTGTTGGC AGGGCTGTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
CGGCAGGCGT CCCTGCATGT CTGGAAGATT GTTGTCTCCA ATACCCCCCG CACCTTGCGT 
GAGATCCTAC CCACTCTCTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCGAG AACATTGGGA GATCTTGTGC GGAAGTTAGG GGAGAAAATC 
CTCCCCGAGA TCATCCCCAT CCTTGAGGAA GGCCTGAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGTGTGT GCATTGGCCT AAGTGAGATC ATGAAGTCCA CCAGCCGGGA TGCCGTGCTG 
TATTTCTCTG AATCCCTCGT GCCCACGGCA AGGAAGGCTT TGTGTGACCC ACTGGAGGAG 
GTCAGAGAGG CGGCAGCCAA GACTTTCGAG CAGCTGCATT CCACCATCGG CCACCAGGCT 
CTGGAGGACA TTCTCCCATT TTTACTAAAG CAGCTGGATG ACGAGGAGGT GTCAGAGTTT 
GCCTTGGATG GTCTGAAGCA AGTCATGGCT ATTAAGAGTC GTGTGGTGCT GCCCTACCTT 
GTGCCCAAGC TGACAACGCC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT TTCGTCAGTG 
GCTGGTGATG CCCTCACCCG TCATCTTGGC GTGATCCTCC CAGCGGTCAT GCTGGCCCTG 
AAGGAAAAGC TTGGGACCCC AGATGAGCAG CTGGAGATGG CCAATTGTCA GGCTGTGATC 
CTCTCCGTAG AGGATGACAC AGGGCACCGG ATCATCATCG AGGATCTGCT GGAGGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAAGCT GCTGCCATCA TCCTCAACAT CTACTGTTCC 
CGCTCAAAGG CTGACTACAC CAGCCACCTG CGGAGCCTGG TCTCGGGCCT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ATGCCCTAAA TGCCATCACT 
AAGAAGCTGG ATGCTGGCAA CCAGTTGGCA CTCATTGAAG AGCTGCACAA GGAAATCCGG 
CTCATAGGGA ACGAGAGCAA AGGCGAGCAT GTGCCAGGAT TCTGCCTCCC GAAGAAGGGA 
GTGACCTCCA TCCTTCCAGT GTTGCGGGAA GGAGTCCTGA CTGGCAGCCC TGAGCAGAAG 
GAGGAGGCAG CCAAAGCCTT AGGCTTGGTA ATCCGCCTGA CCTCGGCTGA CGCCCTGAGG 
CCCTCCGTGG TCAGCATCAC TGGCCCTCTG ATCCGCATCC TGGGGGACAG GTTCAGCTGG 
AATGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGT TGGCTAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACTTTCACCA AAGCCCTGCA GGACTCCAAC 
CGGGGGGTGC GCCTGAAGGC CGCAGATGCT CTGGGGAAGC TCATTTCCAT CCACATTAAG 
GTGGACCCCC TCTTCACAGA GCTGCTCAAT GGCATCCGCG CCATGGAGGA CCCAGGTGTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCAGGGGC CAAAGTGGAT 
GCCGTCATCC GGAAAAACAT CGTCTCACTC CTGCTGAGCA TGCTGGGACA CGATGAGGAC 
AACACTCGCA TCTCCTCAGC CGGGTGCCTA GGGGAACTGT GTGCCTTTTT GACTGAAGAG 
GAGCTTAGTG CCGTTCTACA GCAGTGCTTG CTGGCGGACG TGTCCGGCAT TGACTGGATG 
GTTCGGCACG GGCGGAGCCT GGCACTTTCC GTGGCTGTGA ATGTGGCTCC TGGCAGACTT 
TGTGCCGGCA GATATAGCAG TGATGTTCAG GAAATGATCC TGAGCAGTGC CACGGCGGAC 
AGGATCCCCA TTGCGGTGAG CGGGGTCCGG GGCATGGGCT TTCTCATGAG ACACCACATC 
GAGACAGGCG GAGGGCAGTT GCCGGCCAAA CTTTCCAGCC TGTTCGTTAA GTGTCTGCAG 
AACCCATCCA GCGACATCAG GCTGGTGGCT GAGAAGATGA TCTGGTGGGC AAATAAGGAC 
CCACTGCCTC CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT TCTTGACAAC 
ACCAAGGATA AGAACACCGT GGTCAGGGCC TACAGCGACC AGGCAATTGT CAACCTCCTC 
AAGATGCGGC AGGGTGAAGA GGTGTTTCAG TCCCTCTCCA AGATCCTGGA TGTGGCCAGT 
TTGGAGGTGC TGAACGAGGT TAACCGAAGG TCCCTGAAGA AGCTGGCCAG CCAGGCCGAC 
TCCACGGAGC AGGTGGACGA CACCATCCTG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006827.1
CDS	19..8034
Translation	MAADTQVSETLKRFAGKVTTASVKERREILSELGKCVAGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGIGSKAGVPSKSSGSAALLALTWTCLLVRIVFPSRAKRQGDIWNKLVEVQCLLLLEVLGGSHKHAVDGAVKKLTKLWKENPGLVEQYLSAILSLEPNQNYAGMLGLLVQFCTSHKEMDVVSQHKSALLDFYMKNILMSKVKPPKYLLDSCAPLLRYLSHSEFKDLILPTIQKSLLRSPENVIETISSLLASVTLDLSQYAMDIVKGLAGHLKSNSPRLMDEAVLALRNLARQCSDSSAMESLTKHLFAILGGSEGKLTVVAQKMSVLSGIGSVSHHVVSGPSSQVLNGIVAELFIPFLQQEVHEGTLVHAVSVLALWCNRFTMEVPKKLTEWFKKAFSLKTSTSAVRHAYLQCMLASYRGDTLLQALDLLPLLIQTVEKAASQSTQVPTITEGVAAALLLLKLSVADSQAEAKLSSFWQLIVDEKKQVFTSEKFLVMASEDALCTVLHLTERLFLDHPHRLTGNKVQQYHRALVAVLLSRTWHVRRQAQQTVRKLLSSLGGFKLAHGLLEELKTVLSSHKVLPLEALVTDAGEVTEAGKAYVPPRVLQEALCVISGVPGLKGDVTDTEQLAQEMLIISHHPSLVAVQSGLWPALLARMKIDPEAFITRHLDQIIPRMTTQSPLNQSSMNAMGSLSVLSPDRVLPQLISTITASVQNPALRLVTREEFAIMQTPAGELYDKSIIQSAQQDSIKKANMKRENKAYSFKEQIIELELKEEIKKKKGIKEEVQLTSKQKEMLQAQLDREAQVRRRLQELDGELEAALGLLDIILAKNPSGLTQYIPVLVDSFLPLLKSPLAAPRIKNPFLSLAACVMPSRLKALGTLVSHVTLRLLKPECVLDKSWCQEELSVAVKRAVMLLHTHTITSRVGKGEPGAAPLSAPAFSLVFPFLKMVLTEMPHHSEEEEEWMAQILQILTVQAQLRASPNTPPGRVDENGPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTTLCASSSGDDGCAFAEQEEVDVLLCALQSPCASVRETVLRGLMELHMVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAERLWSMMGLDLQPDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVPDALNDRHPDVRKCMLDAALATLNTHGKENVNSLLPVFEEFLKNAPNDASYDAVRQSVVVLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAIKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVERRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAIKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNIYCSRSKADYTSHLRSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGNQLALIEELHKEIRLIGNESKGEHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALRPSVVSITGPLIRILGDRFSWNVKAALLETLSLLLAKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISIHIKVDPLFTELLNGIRAMEDPGVRDTMLQALRFVIQGAGAKVDAVIRKNIVSLLLSMLGHDEDNTRISSAGCLGELCAFLTEEELSAVLQQCLLADVSGIDWMVRHGRSLALSVAVNVAPGRLCAGRYSSDVQEMILSSATADRIPIAVSGVRGMGFLMRHHIETGGGQLPAKLSSLFVKCLQNPSSDIRLVAEKMIWWANKDPLPPLDPQAIKPILKALLDNTKDKNTVVRAYSDQAIVNLLKMRQGEEVFQSLSKILDVASLEVLNEVNRRSLKKLASQADSTEQVDDTILT

Target ORF information:

RefSeq Version	NM_006836.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GCN1 activator of EIF2AK4 (GCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006836.2

ORF Insert Sequence:

ATGGCGGCGG ACACGCAGGT TTCCGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACAACA 
GCCAGTGTAA AGGAACGGAG AGAAATCCTC AGTGAACTTG GGAAGTGTGT TGCTGGAAAA 
GATCTTCCAG AGGGAGCAGT GAAGGGGCTC TGCAAATTGT TCTGCTTGAC TCTGCATCGA 
TATAGAGATG CAGCCTCCCG CAGGGCCTTG CAGGCAGCCA TCCAGCAGTT GGCTGAGGCC 
CAGCCAGAAG CCACTGCTAA GAACCTTCTA CACTCTCTGC AGTCTTCTGG TATAGGCTCC 
AAAGCAGGTG TTCCCAGTAA GAGCAGTGGC TCTGCCGCCT TGCTGGCCTT GACCTGGACC 
TGCCTCCTGG TGCGCATTGT CTTTCCATCG AGAGCCAAGC GACAAGGAGA CATCTGGAAC 
AAACTGGTGG AAGTGCAGTG CCTGCTCTTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGATG GTGCTGTGAA GAAACTCACG AAGCTGTGGA AAGAGAACCC CGGGCTGGTG 
GAACAGTACT TGTCAGCCAT TCTCAGCCTA GAGCCCAACC AGAACTATGC TGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGTCAC AAGGAGATGG ACGTGGTCAG TCAGCACAAG 
AGCGCCCTAC TGGACTTTTA CATGAAGAAC ATCCTGATGA GCAAAGTCAA GCCTCCGAAG 
TACCTGTTGG ATAGCTGTGC CCCTCTGCTC CGATACCTGT CCCACTCAGA ATTTAAGGAT 
CTGATACTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT TATTGAAACT 
ATTTCTAGTC TGCTGGCATC AGTGACGCTT GACCTCAGCC AGTATGCCAT GGACATCGTG 
AAAGGACTGG CTGGTCACCT GAAATCCAAC AGTCCCCGCC TGATGGATGA AGCTGTGCTG 
GCACTGCGGA ACCTGGCACG CCAGTGCAGT GACTCTTCGG CCATGGAATC CCTGACCAAG 
CACCTATTTG CTATCCTCGG AGGCTCGGAA GGAAAACTAA CTGTTGTAGC CCAGAAGATG 
AGCGTCCTCT CAGGGATTGG GAGCGTCAGT CATCACGTGG TGTCTGGACC TTCCAGTCAG 
GTCCTGAATG GGATCGTGGC TGAGCTGTTC ATCCCGTTCC TTCAGCAGGA AGTTCATGAA 
GGGACCTTGG TACACGCTGT CTCAGTCCTG GCTCTCTGGT GTAACCGATT CACTATGGAA 
GTGCCCAAGA AGCTCACTGA ATGGTTCAAA AAAGCTTTCA GCCTTAAAAC CTCCACATCT 
GCGGTGAGGC ATGCCTACCT GCAGTGCATG TTGGCCTCTT ACCGGGGTGA CACGCTGTTG 
CAGGCCCTGG ACTTACTGCC CTTGCTCATC CAGACAGTGG AGAAGGCAGC CTCCCAAAGC 
ACTCAGGTTC CCACCATCAC TGAAGGGGTT GCCGCAGCCT TGTTGCTCTT AAAGTTGTCA 
GTGGCTGACT CACAGGCTGA GGCCAAACTG AGCAGTTTCT GGCAGTTGAT TGTGGATGAG 
AAAAAGCAGG TTTTCACTTC TGAGAAATTC CTGGTCATGG CTTCAGAGGA TGCCCTGTGT 
ACTGTGTTGC ATCTGACAGA GAGACTTTTC CTTGACCACC CGCATAGACT CACTGGCAAC 
AAAGTTCAGC AGTACCACCG GGCTCTGGTG GCGGTGCTCC TGAGCCGCAC CTGGCACGTC 
CGCAGGCAGG CTCAGCAGAC AGTTCGGAAG CTGCTGTCCT CTCTTGGGGG CTTTAAGCTG 
GCGCACGGAC TCTTGGAGGA GCTGAAGACT GTCCTCAGTT CTCACAAGGT GCTGCCCTTA 
GAGGCTTTGG TGACTGATGC TGGAGAGGTG ACTGAGGCAG GCAAGGCCTA CGTGCCTCCA 
CGGGTCCTGC AGGAGGCTCT GTGTGTCATC TCCGGTGTGC CAGGGCTCAA GGGTGATGTC 
ACCGACACTG AACAACTGGC CCAGGAAATG CTGATCATCT CCCACCACCC ATCCTTAGTT 
GCCGTGCAGT CTGGACTTTG GCCAGCACTT CTTGCCAGGA TGAAGATCGA TCCTGAAGCC 
TTTATCACCA GGCACCTGGA TCAGATCATT CCCAGGATGA CCACACAGAG TCCCCTAAAC 
CAGTCCTCCA TGAATGCCAT GGGCTCCCTT TCCGTCCTGT CGCCGGACCG GGTCCTCCCA 
CAGCTCATCA GCACCATCAC TGCCTCCGTG CAGAACCCTG CACTGCGCCT GGTGACGCGG 
GAGGAGTTTG CCATTATGCA GACCCCTGCT GGGGAGCTGT ATGACAAATC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTATTCC 
TTCAAAGAGC AGATCATCGA GCTGGAGCTG AAGGAGGAGA TAAAGAAGAA GAAAGGCATC 
AAAGAGGAGG TGCAGCTGAC CAGCAAGCAG AAGGAGATGC TGCAGGCCCA GCTAGACAGG 
GAGGCGCAGG TCCGGAGGCG GCTGCAGGAG CTGGATGGGG AGCTGGAGGC GGCGCTTGGA 
CTGCTGGACA TCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCTGTTTTG 
GTCGACTCTT TTCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGAACCCC 
TTCTTGTCCT TGGCTGCCTG TGTCATGCCC TCTAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCACGTGA CCCTGCGCCT GCTGAAGCCA GAGTGTGTCC TGGATAAGTC CTGGTGCCAG 
GAAGAGCTGT CGGTGGCTGT GAAGAGGGCG GTGATGCTGC TGCACACCCA CACCATCACC 
AGCAGGGTGG GCAAGGGGGA GCCAGGTGCT GCGCCCTTGT CCGCGCCAGC CTTCTCCTTA 
GTCTTCCCGT TTCTGAAGAT GGTGCTGACG GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAGTGGATGG CCCAGATTCT TCAGATCCTC ACTGTCCAAG CCCAGCTGAG GGCCTCCCCC 
AACACCCCAC CCGGGCGGGT GGACGAGAAT GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGTCTTC TGACTTGGGT GATCGGGACG GGCTCGCCTC GCTTACAGGT TCTGGCTTCA 
GACACCCTGA CCACCCTGTG TGCCAGCAGC AGTGGTGATG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGTGCC TTGCAGTCCC CGTGTGCCAG CGTGCGGGAA 
ACCGTGCTCC GGGGGCTGAT GGAACTCCAC ATGGTATTGC CAGCACCTGA TACTGATGAG 
AAGAATGGCC TGAACCTTCT GCGGAGACTC TGGGTGGTCA AGTTTGACAA GGAGGAGGAG 
ATCCGGAAGC TGGCTGAGAG GCTCTGGTCA ATGATGGGCC TAGACCTGCA GCCAGACCTC 
TGCTCCTTGC TGATTGACGA CGTGATCTAT CATGAGGCGG CTGTAAGGCA GGCAGGGGCC 
GAAGCCCTCT CCCAAGCAGT GGCACGTTAC CAGCGGCAGG CGGCGGAGGT TATGGGCAGG 
CTCATGGAGA TTTACCAGGA AAAGCTCTAC CGGCCGCCCC CAGTGCTGGA TGCTTTGGGA 
CGAGTTATTT CAGAATCTCC TCCAGATCAG TGGGAAGCCA GGTGTGGCTT GGCGTTGGCC 
CTCAACAAGC TCTCCCAGTA TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAGTTTTTT 
GTCCCTGATG CCCTCAATGA CCGACACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCC 
CTCGCAACGC TCAACACTCA TGGGAAGGAG AACGTCAACT CGCTGTTGCC AGTATTCGAG 
GAGTTCCTGA AGAACGCGCC CAATGATGCC AGCTATGATG CTGTGCGACA GAGTGTGGTG 
GTCCTGATGG GCTCTCTGGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCCATT 
GTTGCCAAGC TCATCGCTGC CCTCTCCACC CCCTCCCAGC AGGTCCAGGA GTCCGTAGCC 
AGCTGCTTGC CACCCCTTGT GCCAGCCATC AAGGAGGATG CTGGAGGGAT GATCCAGAGG 
CTTATGCAGC AGCTGCTGGA GTCAGACAAG TACGCAGAGC GCAAAGGGGC CGCCTATGGC 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTCTCGCTGA AGCAACAGGA GATGATGGCG 
GCACTGACTG ATGCCATCCA AGATAAGAAG AACTTCCGCC GGCGAGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAACTTTTTG AGCCGTATGT GGTTCACGTG 
CTGCCCCATC TGCTCCTGTG CTTTGGGGAT GGAAACCAGT ATGTGCGTGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACTTG AGTGCTCACG GGGTGAAGCT GGTGCTCCCC 
TCCTTACTGG CTGCCCTGGA GGAGGAATCG TGGCGGACCA AAGCTGGGTC AGTGGAGCTT 
CTTGGGGCAA TGGCGTACTG TGCTCCTAAG CAGCTGTCAT CCTGTCTACC CAACATTGTG 
CCCAAGCTTA CGGAGGTGCT GACCGACTCC CATGTCAAAG TCCAGAAGGC TGGACAGCAG 
GCGCTCAGGC AGATCGGCTC CGTTATCAGG AACCCGGAGA TCCTGGCCAT TGCTCCAGTC 
CTCCTGGATG CCCTGACGGA TCCCTCCAGG AAGACCCAGA AGTGCTTGCA GACCCTGCTG 
GACACCAAGT TTGTCCACTT CATTGATGCC CCATCCCTGG CCCTCATCAT GCCCATTGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACGCGGAAGA TGGCAGCCCA GATTATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCGT ACCTGCCCAG CGTGACGCCT 
GGCCTGAAAG CATCGCTTTT GGACCCTGTG CCTGAGGTGC GGACCGTATC TGCAAAGGCC 
CTTGGGGCCA TGGTGAAGGG CATGGGGGAG TCGTGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTATGA GCAGAGCTCT GTGGATCGCT CAGGCGCTGC ACAGGGGTTG 
GCTGAGGTCA TGGCCGGTTT GGGGGTGGAG AAGTTGGAGA AGTTGATGCC AGAAATCGTG 
GCTACAGCCA GCAAAGTGGA CATTGCACCC CATGTCCGAG ATGGCTACAT TATGATGTTT 
AACTACCTGC CCATCACCTT TGGAGACAAG TTTACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAT GAGTTTGTGC GTGACACCGC CCTGCGCGCG 
GGCCAGCGGG TTATCTCCAT GTACGCTGAG ACAGCCATCG CCCTGCTGCT GCCCCAGCTA 
GAGCAAGGCC TCTTTGATGA CCTTTGGAGA ATCAGGTTCA GCTCTGTTCA GCTCCTTGGG 
GATCTCCTGT TTCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCTGAG 
GATGATAACT TTGGAACTGC CCAGTCCAAC AAGGCGATCA TCACTGCCCT GGGGGTAGAG 
CGGCGGAACC GGGTGTTGGC AGGGCTGTAC ATGGGCCGCT CAGACACCCA GCTGGTGGTG 
CGGCAGGCGT CCCTGCATGT CTGGAAGATT GTTGTCTCCA ATACCCCCCG CACCTTGCGT 
GAGATCCTAC CCACTCTCTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC GTGTGCAGAT 
AAGAGAACGA TTGCAGCGAG AACATTGGGA GATCTTGTGC GGAAGTTAGG GGAGAAAATC 
CTCCCCGAGA TCATCCCCAT CCTTGAGGAA GGCCTGAGGT CTCAGAAGAG CGATGAGAGG 
CAGGGTGTGT GCATTGGCCT AAGTGAGATC ATGAAGTCCA CCAGCCGGGA TGCCGTGCTG 
TATTTCTCTG AATCCCTCGT GCCCACGGCA AGGAAGGCTT TGTGTGACCC ACTGGAGGAG 
GTCAGAGAGG CGGCAGCCAA GACTTTCGAG CAGCTGCATT CCACCATCGG CCACCAGGCT 
CTGGAGGACA TTCTCCCATT TTTACTAAAG CAGCTGGATG ACGAGGAGGT GTCAGAGTTT 
GCCTTGGATG GTCTGAAGCA AGTCATGGCT ATTAAGAGTC GTGTGGTGCT GCCCTACCTT 
GTGCCCAAGC TGACAACGCC ACCTGTCAAC ACCCGGGTGC TGGCTTTCCT TTCGTCAGTG 
GCTGGTGATG CCCTCACCCG TCATCTTGGC GTGATCCTCC CAGCGGTCAT GCTGGCCCTG 
AAGGAAAAGC TTGGGACCCC AGATGAGCAG CTGGAGATGG CCAATTGTCA GGCTGTGATC 
CTCTCCGTAG AGGATGACAC AGGGCACCGG ATCATCATCG AGGATCTGCT GGAGGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAAGCT GCTGCCATCA TCCTCAACAT CTACTGTTCC 
CGCTCAAAGG CTGACTACAC CAGCCACCTG CGGAGCCTGG TCTCGGGCCT GATCCGCCTC 
TTCAATGACT CCAGCCCTGT GGTTCTGGAG GAGAGCTGGG ATGCCCTAAA TGCCATCACT 
AAGAAGCTGG ATGCTGGCAA CCAGTTGGCA CTCATTGAAG AGCTGCACAA GGAAATCCGG 
CTCATAGGGA ACGAGAGCAA AGGCGAGCAT GTGCCAGGAT TCTGCCTCCC GAAGAAGGGA 
GTGACCTCCA TCCTTCCAGT GTTGCGGGAA GGAGTCCTGA CTGGCAGCCC TGAGCAGAAG 
GAGGAGGCAG CCAAAGCCTT AGGCTTGGTA ATCCGCCTGA CCTCGGCTGA CGCCCTGAGG 
CCCTCCGTGG TCAGCATCAC TGGCCCTCTG ATCCGCATCC TGGGGGACAG GTTCAGCTGG 
AATGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGT TGGCTAAGGT TGGGATTGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACTTTCACCA AAGCCCTGCA GGACTCCAAC 
CGGGGGGTGC GCCTGAAGGC CGCAGATGCT CTGGGGAAGC TCATTTCCAT CCACATTAAG 
GTGGACCCCC TCTTCACAGA GCTGCTCAAT GGCATCCGCG CCATGGAGGA CCCAGGTGTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCAGGGGC CAAAGTGGAT 
GCCGTCATCC GGAAAAACAT CGTCTCACTC CTGCTGAGCA TGCTGGGACA CGATGAGGAC 
AACACTCGCA TCTCCTCAGC CGGGTGCCTA GGGGAACTGT GTGCCTTTTT GACTGAAGAG 
GAGCTTAGTG CCGTTCTACA GCAGTGCTTG CTGGCGGACG TGTCCGGCAT TGACTGGATG 
GTTCGGCACG GGCGGAGCCT GGCACTTTCC GTGGCTGTGA ATGTGGCTCC TGGCAGACTT 
TGTGCCGGCA GATATAGCAG TGATGTTCAG GAAATGATCC TGAGCAGTGC CACGGCGGAC 
AGGATCCCCA TTGCGGTGAG CGGGGTCCGG GGCATGGGCT TTCTCATGAG ACACCACATC 
GAGACAGGCG GAGGGCAGTT GCCGGCCAAA CTTTCCAGCC TGTTCGTTAA GTGTCTGCAG 
AACCCATCCA GCGACATCAG GCTGGTGGCT GAGAAGATGA TCTGGTGGGC AAATAAGGAC 
CCACTGCCTC CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCTCT TCTTGACAAC 
ACCAAGGATA AGAACACCGT GGTCAGGGCC TACAGCGACC AGGCAATTGT CAACCTCCTC 
AAGATGCGGC AGGGTGAAGA GGTGTTTCAG TCCCTCTCCA AGATCCTGGA TGTGGCCAGT 
TTGGAGGTGC TGAACGAGGT TAACCGAAGG TCCCTGAAGA AGCTGGCCAG CCAGGCCGAC 
TCCACGGAGC AGGTGGACGA CACCATCCTG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response.
Journal of cell science129(24)4521-4533(2016 12)
Silva RC,Sattlegger E,Castilho BA

Interactome analysis of herpes simplex virus 1 envelope glycoprotein H.
Microbiology and immunology59(6)331-7(2015 Jun)
Hirohata Y,Kato A,Oyama M,Kozuka-Hata H,Koyanagi N,Arii J,Kawaguchi Y

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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GCN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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