E2f1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2f1
Entrez Gene ID	399489
Full Name	E2F transcription factor 1
General protein information	Preferred Names E2F transcription factor 1 Names transcription factor E2F1 E2F-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	transcription factor; involved in controling the G1/S transition of eukaryotic cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100778.1	NP_001094248.1	transcription factor E2F1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC101885420	XP_005174590.1
Macaca mulatta (Rhesus monkey)	E2F1	XP_001103717.2
Pan troglodytes (chimpanzee)	E2F1	XP_003316910.1
Homo sapiens (human)	E2F1	NP_005216.1
Rattus norvegicus (Norway rat)	E2f1	NP_001094248.1
Xenopus tropicalis (tropical clawed frog)	e2f1	XP_002932903.1
Canis lupus familiaris (dog)	E2F1	XP_005635002.1
Bos taurus (cattle)	E2F1	NP_001193008.1
Mus musculus (house mouse)	E2f1	NP_031917.1
Gallus gallus (chicken)	E2F1	NP_990550.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of two novel alternatively spliced E2F1 transcripts in the rat CNS.
Jackson DP, Ting JH, Pozniak PD, Meurice C, Schleidt SS, Dao A, Lee AH, Klinman E, Jordan-Sciutto KL
Molecular and cellular neurosciences921-11(2018 10)

Tumour growth-suppressive effect of arsenic trioxide in squamous cell lung carcinoma.
Leung LL, Lam SK, Li YY, Ho JC
Oncology letters14(3)3748-3754(2017 Sep)

The over expression of long non-coding RNA ANRIL promotes epithelial-mesenchymal transition by activating the ATM-E2F1 signaling pathway in pancreatic cancer: An in vivo and in vitro study.
Chen S, Zhang JQ, Chen JZ, Chen HX, Qiu FN, Yan ML, Chen YL, Peng CH, Tian YF, Wang YD
International journal of biological macromolecules102718-728(2017 Sep)

Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2).
Xia G, Wang H, Song Z, Meng Q, Huang X, Huang X
Journal of experimental & clinical cancer research : CR36(1)107(2017 08)

TRIM28 knockdown increases sensitivity to etoposide by upregulating E2F1 in non-small cell lung cancer.
Liu L, Xiao L, Liang X, Chen L, Cheng L, Zhang L, Wu X, Xu Q, Ma C
Oncology reports37(6)3597-3605(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E2f1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12360	NM_001100778.1 Latest version!	Rattus norvegicus E2F transcription factor 1 (E2f1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2f1 ( NM_001100778.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094248.1 Rattus norvegicus E2F transcription factor 1 (E2f1), mRNA.

Price & Availability↑

CloneID	ORa12360
Clone ID Related Accession (Same CDS sequence)	NM_001100778.1
Accession Version	NM_001100778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor E2F1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07054363.1. On or before Jun 1, 2008 this sequence version replaced XM_230765.4, XM_001065036.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906350, SAMN01906352 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGTAG CCCCCGCGGG CGGCCAGCAC GCGCCGGCGC TGGAGGCCCT GCTCGGGGCG 
GGCGCGCTGC GGCTGCTCGA CTCCTCGCAG ATCGTCATCA TCTCCACCGC GCCCGATGTC 
GGCGCCCCGC AGGTCCCCAC CGGCCCCGCC GCGCCGCCCG CTGGCCCTCG AGATCCTGAC 
GTGCTGCTCT TCGCAACGCC GCAGGCGCCC CGACCCGCGC CTAGTGCACC GCGCCCGGCT 
CTCGGCCGCC CGCCGGTGAA ACGGAGGCTG GATCTGGAAA CTGACCATCA GTACCTTGCT 
GGTAGCAGCG GGCCATTCCG GGGCAGAGGC CGCCACCCAG GGAAAGGTGT GAAATCTCCA 
GGGGAGAAGT CACGCTATGA GACCTCACTA AATCTGACCA CCAAACGCTT CTTGGAGCTG 
CTGAGCCACT CAGCTGATGG TGTTGTTGAT CTGAACTGGG CAGCCGAGGT GCTGAAGGTG 
CAGAAACGAC GCATCTATGA CATCACCAAT GTCCTGGAGG GCATCCAACT CATTGCCAAG 
AAGTCCAAGA ATCATATCCA GTGGCTAGGC AGCCGCACCA TGGTGGGGAT CGGTCAGCGG 
CTTGAAGGCC TGACCCAGGA CCTGCAACAA CTGCAGGAGA GTGAGCAGCA GCTGGATCAC 
CTGATGCACA TCTGTACCAC TCAGCTGCAA CTGCTTTCTG AGGACTCAGA CATCCAGCGC 
CTGGCCTATG TGACCTGCCA AGATCTCCGC AGCATTGCAG ACCCTGCAGA ACAAATGGTC 
ATAGTGATCA AGGCCCCTCC TGAGACCCAA CTACAAGCTG TGGATTCTGC AGAGACATTT 
CAGATCTCCC TTAAGAGCAA ACAAGGCCCC ATCGATGTTT TCCTGTGCCC TGAGGAAAGT 
GCAGAGGGGA TTAGCCCTGG GAGGACCTCA TACCAGGAGA CATCTGGGGA GGACAGGAAT 
GCTGACTCTG GCACAGCAGG GCCTCCACCA TCACCTCCCT CCACATCCCC AACCTTGGAT 
CCCAGCCAGT CCCTGTTAGG CCTGGAGCAA GAAGCTGTAT TGCCTCGAAT AGGCAACCTG 
AGGGCCCCCA TGGAAGAAGA CCGGTTGTCA CCACTGGTGG CTGCTGACTC ACTCCTGGAG 
CATGTTAAAG AAGACTTCTC TGGGCTCCTC CCTGGGGAGT TCATCAGCCT TTCCCCACCC 
CATGAGGCTG TTGACTATCA CTTTGGTCTC GAGGAGGGTG AGGGCATTAG AGATCTCTTT 
GACTGTGACT TTGGGGACTT GACCCCTCTG GATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094248.1
CDS	154..1452
Translation	MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQVPTGPAAPPAGPRDPDVLLFATPQAPRPAPSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSRTMVGIGQRLEGLTQDLQQLQESEQQLDHLMHICTTQLQLLSEDSDIQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSAETFQISLKSKQGPIDVFLCPEESAEGISPGRTSYQETSGEDRNADSGTAGPPPSPPSTSPTLDPSQSLLGLEQEAVLPRIGNLRAPMEEDRLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEAVDYHFGLEEGEGIRDLFDCDFGDLTPLDF

Target ORF information:

RefSeq Version	NM_001100778.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus E2F transcription factor 1 (E2f1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100778.1

ORF Insert Sequence:

ATGGCCGTAG CCCCCGCGGG CGGCCAGCAC GCGCCGGCGC TGGAGGCCCT GCTCGGGGCG 
GGCGCGCTGC GGCTGCTCGA CTCCTCGCAG ATCGTCATCA TCTCCACCGC GCCCGATGTC 
GGCGCCCCGC AGGTCCCCAC CGGCCCCGCC GCGCCGCCCG CTGGCCCTCG AGATCCTGAC 
GTGCTGCTCT TCGCAACGCC GCAGGCGCCC CGACCCGCGC CTAGTGCACC GCGCCCGGCT 
CTCGGCCGCC CGCCGGTGAA ACGGAGGCTG GATCTGGAAA CTGACCATCA GTACCTTGCT 
GGTAGCAGCG GGCCATTCCG GGGCAGAGGC CGCCACCCAG GGAAAGGTGT GAAATCTCCA 
GGGGAGAAGT CACGCTATGA GACCTCACTA AATCTGACCA CCAAACGCTT CTTGGAGCTG 
CTGAGCCACT CAGCTGATGG TGTTGTTGAT CTGAACTGGG CAGCCGAGGT GCTGAAGGTG 
CAGAAACGAC GCATCTATGA CATCACCAAT GTCCTGGAGG GCATCCAACT CATTGCCAAG 
AAGTCCAAGA ATCATATCCA GTGGCTAGGC AGCCGCACCA TGGTGGGGAT CGGTCAGCGG 
CTTGAAGGCC TGACCCAGGA CCTGCAACAA CTGCAGGAGA GTGAGCAGCA GCTGGATCAC 
CTGATGCACA TCTGTACCAC TCAGCTGCAA CTGCTTTCTG AGGACTCAGA CATCCAGCGC 
CTGGCCTATG TGACCTGCCA AGATCTCCGC AGCATTGCAG ACCCTGCAGA ACAAATGGTC 
ATAGTGATCA AGGCCCCTCC TGAGACCCAA CTACAAGCTG TGGATTCTGC AGAGACATTT 
CAGATCTCCC TTAAGAGCAA ACAAGGCCCC ATCGATGTTT TCCTGTGCCC TGAGGAAAGT 
GCAGAGGGGA TTAGCCCTGG GAGGACCTCA TACCAGGAGA CATCTGGGGA GGACAGGAAT 
GCTGACTCTG GCACAGCAGG GCCTCCACCA TCACCTCCCT CCACATCCCC AACCTTGGAT 
CCCAGCCAGT CCCTGTTAGG CCTGGAGCAA GAAGCTGTAT TGCCTCGAAT AGGCAACCTG 
AGGGCCCCCA TGGAAGAAGA CCGGTTGTCA CCACTGGTGG CTGCTGACTC ACTCCTGGAG 
CATGTTAAAG AAGACTTCTC TGGGCTCCTC CCTGGGGAGT TCATCAGCCT TTCCCCACCC 
CATGAGGCTG TTGACTATCA CTTTGGTCTC GAGGAGGGTG AGGGCATTAG AGATCTCTTT 
GACTGTGACT TTGGGGACTT GACCCCTCTG GATTTCTGA