Ank3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ank3
Entrez Gene ID	361833
Full Name	ankyrin 3
Synonyms	ANK-3
General protein information	Preferred Names ankyrin 3 Names ankyrin-3 ankyrin 3 (G) ankyrin 3, epithelial ankyrin 3, node of Ranvier (ankyrin G) ankyrin-G
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p11
Summary	may play a role in the organization of the sarcoplasmic reticulum and postsynaptic membrane of skeletal muscle fibers [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031805.1	NP_113993.1	ankyrin-3 isoform 1
NM_001033984.1	NP_001029156.1	ankyrin-3 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ANK3	NP_001264026.1
Drosophila melanogaster (fruit fly)	Ank	NP_001162819.1
Danio rerio (zebrafish)	ank3b	XP_005156095.1
Xenopus tropicalis (tropical clawed frog)	ank3	XP_004915849.1
Macaca mulatta (Rhesus monkey)	ANK3	XP_002808549.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009937	XP_319063.4
Homo sapiens (human)	ANK3	NP_066267.2
Pan troglodytes (chimpanzee)	ANK3	XP_003312618.1
Mus musculus (house mouse)	Ank3	NP_666117.2
Rattus norvegicus (Norway rat)	Ank3	NP_001029156.1
Canis lupus familiaris (dog)	ANK3	XP_536358.3

Gene Ontology
Bibliography

Related articles in PubMed

Autoinhibition of ankyrin-B/G membrane target bindings by intrinsically disordered segments from the tail regions.
Chen K, Li J, Wang C, Wei Z, Zhang M
eLife6(2017 08)

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, Hoogenraad CC
Neuron94(2)347-362.e7(2017 Apr)

Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.
Sohn PD, Tracy TE, Son HI, Zhou Y, Leite RE, Miller BL, Seeley WW, Grinberg LT, Gan L
Molecular neurodegeneration11(1)47(2016 06)

Structural basis for the membrane association of ankyrinG via palmitoylation.
Fujiwara Y, Kondo HX, Shirota M, Kobayashi M, Takeshita K, Nakagawa A, Okamura Y, Kinoshita K
Scientific reports623981(2016 Apr)

TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.
van Beuningen SFB, Will L, Harterink M, Chazeau A, van Battum EY, Frias CP, Franker MAM, Katrukha EA, Stucchi R, Vocking K, Antunes AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AFM, Post JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, Hoogenraad CC
Neuron88(6)1208-1226(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ank3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ank3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08778	NM_031805.1 Latest version!	Rattus norvegicus ankyrin 3 (Ank3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa08809	NM_001033984.1 Latest version!	Rattus norvegicus ankyrin 3 (Ank3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ank3 ( NM_031805.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113993.1 Rattus norvegicus ankyrin 3 (Ank3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa08778
Clone ID Related Accession (Same CDS sequence)	NM_031805.1
Accession Version	NM_031805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559750400000
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07044767.1, AJ812024.1, AF102552.1 and AJ812019.1. Transcript Variant: This variant (1) has a different 5' UTR and multiple differences in the coding region but maintains the reading frame, compared to variant 2. This variant encodes isoform 1 which is shorter than isoform 2. Isoform 1 is also known as AnkG217. Sequence Note: This RefSeq record was created from genomic and transcript sequence data from multiple strains to make it consistent with the reference genome sequence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ812024.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGAAG AGGCAAAGGA GAAAACCGCC AAGCCTGCTC ATAGGAAGAG GAAAGGGAAA 
AAGTCGGATG CCAATGCAAG TTACTTGAGA GCAGCTCGGG CGGGGCACCT GGAAAAGGCC 
CTTGACTACA TCAAAAATGG AGTGGACGTC AACATCTGTA ACCAGAATGG GTTGAATGCG 
CTCCATCTTG CTTCCAAAGA AGGCCATGTG GAAGTGGTCT CCGAGCTGCT GCAGAGAGAA 
GCCAACGTCG ATGCAGCCAC AAAGAAAGGA AACACAGCCT TACACATCGC ATCCTTGGCT 
GGACAAGCGG AAGTGGTCAA GGTCTTGGTT ACGAACGGAG CGAACGTCAA CGCACAGTCT 
CAGAATGGCT TCACGCCGCT GTATATGGCG GCCCAGGAGA ACCACCTGGA AGTCGTCAGG 
TTTCTTCTGG ACAATGGTGC CAGCCAAAGC CTGGCTACAG AGGATGGCTT CACGCCATTG 
GCCGTGGCTC TTCAACAAGG TCACGACCAA GTCGTATCCC TCCTGCTCGA GAACGACACG 
AAGGGAAAAG TGCGCCTCCC AGCCCTCCAC ATCGCGGCCC GGAAAGATGA CACCAAAGCG 
GCAGCTCTGC TCCTACAGAA CGACACCAAT GCAGACATAG AGTCAAAGAG TGGCTTCACC 
CCACTCCACA TAGCTGCCCA CTACGGGAAT ATCAATGTGG CCACGTTGCT GTTAAACCGA 
GCGGCTGCCG TGGACTTCAC CGCACGGAAT GACATCACTC CTTTGCATGT TGCCTCGAAA 
CGGGGAAACG CAAATATGGT AAAGCTGTTG CTCGACCGGG GTGCGAAGAT CGACGCCAAG 
ACCAGGGACG GTCTGACCCC GCTGCACTGC GGGGCGAGAA GTGGCCACGA GCAGGTGGTA 
GAGATGTTGC TGGACAGAGC CGCCCCCATC CTTTCGAAAA CCAAGAATGG ACTGTCCCCA 
CTGCACATGG CCACACAAGG AGACCATTTA AACTGCGTCC AACTCCTCCT CCAGCACAAC 
GTGCCCGTGG ATGACGTCAC CAACGACTAC CTGACCGCCC TCCACGTGGC GGCCCACTGC 
GGCCATTACA AAGTTGCCAA GGTTCTTTTG GATAAGAAAG CTAACCCCAA TGCCAAAGCT 
CTGAATGGCT TCACCCCTCT CCATATCGCC TGCAAAAAGA ACCGAATCCG AGTAATGGAA 
CTCCTTTTGA AGCACGGTGC ATCTATTCAA GCCGTAACCG AGTCGGGCCT TACCCCAATC 
CATGTCGCTG CCTTCATGGG ACATGTAAAT ATCGTGTCAC AGCTAATGCA TCATGGAGCC 
TCCCCAAACA CCACCAACGT GAGAGGAGAA ACAGCATTGC ATATGGCGGC TCGGTCGGGG 
CAAGCAGAAG TGGTGCGGTA TTTGGTACAA GATGGAGCTC AGGTAGAAGC AAAAGCTAAG 
GATGACCAGA CCCCACTCCA CATTTCAGCC CGACTCGGGA AGGCTGACAT AGTGCAACAA 
CTGTTACAGC AAGGAGCGTC CCCGAATGCA GCAACAACTT CCGGGTACAC CCCCCTTCAC 
CTTTCGGCCA GAGAGGGGCA TGAGGATGTA GCTGCCTTCC TTCTGGACCA TGGAGCATCT 
TTATCCATAA CAACAAAGAA GGGATTCACG CCTCTGCACG TGGCTGCAAA ATATGGGAAG 
CTTGAAGTGG CAAGTCTCCT GCTGCAGAAG AGTGCGTCTC CTGATGCCGC TGGGAAGAGC 
GGGCTAACTC CACTGCATGT AGCAGCACAT TACGATAATC AGAAAGTGGC CCTTCTGCTT 
TTGGACCAGG GAGCCTCACC CCACGCAGCT GCAAAGAACG GTTATACGCC ACTGCACATC 
GCCGCCAAGA AGAACCAGAT GGACATAGCC ACGTCCCTGC TGGAGTACGG AGCTGATGCA 
AACGCCGTTA CCCGGCAAGG GATTGCGTCC GTCCACCTTG CAGCGCAGGA AGGGCACGTG 
GACATGGTGT CGCTGCTCCT CAGTAGAAAT GCGAACGTCA ACTTGAGCAA TAAGAGCGGC 
CTCACCCCAC TCCACCTGGC GGCTCAAGAA GACCGAGTGA ACGTGGCTGA GGTCCTTGTC 
AACCAAGGGG CCCACGTGGA TGCACAGACA AAGATGGGCT ACACGCCGCT CCATGTGGGC 
TGTCACTATG GAAACATCAA AATAGTCAAT TTTCTGCTGC AGCATTCTGC GAAAGTTAAT 
GCCAAGACAA AGAATGGATA CACGCCACTG CACCAGGCAG CCCAGCAGGG CCACACACAC 
ATCATCAACG TCCTGCTCCA GAACAACGCT TCCCCCAATG AACTCACTGT GAATGGGAAC 
ACAGCCCTGG CCATCGCCCG ACGCCTTGGC TACATCTCGG TGGTTGACAC CCTAAAGGTC 
GTGACAGAGG AAATTATGAC CACCACTACC ATCACGGAGA AGCACAAAAT GAATGTCCCA 
GAAACGATGA ATGAAGTCCT CGACATGTCA GACGATGAAG GTGAAGATGC CATCACAGGG 
GACACTGACA AGTATCTCGG GCCACAGGAC CTCAAGGAGC TGGGCGATGA CTCCCTGCCT 
GCCGAGGGCT ATGTGGGCTT CAGTCTCGGA GCCCGTTCTG CCAGTCCCAA GATAAGTTCG 
GATAGGTCCT ACACCCTGAA CAGAAGCTCC TATGCGCGGG ACAGCATGAT GATCGAGGAG 
CTTCTGGTGC CATCCAAAGA GCAGCACCTT CCATTCACGA GGGAGTTTGA CTCCGACTCC 
CTCAGACACT ACAGTTGGGC AGCAGACACA CTGGACAATG TGAACCTGGT CTCCAGCCCT 
GTGCATTCTG GGTTTCTGGT TAGCTTTATG GTGGACGCGA GAGGGGGCTC CATGCGAGGA 
AGCCGCCACC ACGGGATGCG GATCATCATC CCTCCGCGCA AGTGTACGGC CCCCACCCGC 
ATCACTTGCC GCCTGGTAAA GAGACATAAA CTGGCCAACC CACCCCCCAT GGTGGAAGGA 
GAGGGATTAG CCAGTAGGCT GGTAGAAATG GGTCCTGCGG GGGCACAATT TTTAGGCCCG 
GTCATAGTGG AAATCCCTCA TTTTGGGTCC ATGAGAGGAA AGGAGAGGGA ACTCATTGTC 
CTCCGGAGCG AGAACGGAGA GACCTGGAAG GAACACCAGT TTGACAGCAA AAACGAAGAC 
CTCTCGGAGC TCCTCAATGG CATGGACGAA GAACTTGACA GCCCAGAAGA GTTGGGCACA 
AAGCGCATCT GCAGAATCAT CACGAAGGAT TTCCCCCAGT ATTTCGCTGT GGTTTCCCGG 
ATTAAGCAGG AAAGCAACCA GATTGGTCCC GAAGGTGGGA TTCTGAGCAG CACCACGGTC 
CCCCTCGTCC AGGCGTCTTT CCCAGAGGGT GCCTTAACCA AAAGGATCCG TGTGGGTCTG 
CAGGCTCAGC CCGTACCAGA GGAGACTGTG AAAAAGATCC TTGGAAACAA AGCAACGTTT 
AGCCCAATTG TCACGGTGGA GCCGAGGAGG AGGAAATTCC ATAAGCCGAT CACCATGACC 
ATCCCGGTGC CCCCACCCTC GGGAGAAGGC GTGTCCAATG GGTACAAGGG GGACACCACG 
CCCAGCCTGC GACTCCTCTG CAGCATTACA GGAGGGACCT CGCCGGCTCA GTGGGAAGAC 
ATCACAGGCA CAACCCCGCT GACCTTCATA AAGGACTGTG TGTCTTTCAC AACCAATGTT 
TCAGCCAGGT TCTGGCTGGC GGACTGCCAT CAGGTGTTAG AAACCGTAGG GCTAGCCTCG 
CAGCTGTACA GAGAGTTGAT ATGTGTCCCC TACATGGCCA AGTTTGTTGT TTTTGCCAAA 
ACAAACGACC CAGTGGAATC CTCCCTAAGG TGCTTCTGTA TGACAGACGA CAGAGTGGAC 
AAAACCCTGG AGCAGCAGGA GAATTTCGAG GAGGTTGCCA GAAGCAAAGA CATCGAGGTT 
CTGGAAGGAA AGCCCATCTA TGTTGATTGC TATGGAAACC TGGCTCCTCT GACCAAAGGA 
GGGCAGCAGC TTGTTTTTAA CTTCTATTCT TTCAAAGAAA ACAGACTGCC ATTTTCCATC 
AAGGTTAGAG ACACCAGCCA AGAGCCCTGC GGCCGTCTGT CTTTCCTGAA GGAGCCGAAA 
ACAACAAAAG GATTACCCCA AACGGCTGTT TGCAACTTAA ATATTACTCT ACCGGCGCAT 
AAAAAGGCTG AGAAGGCAGA CAGACGCCAG AGCTTCACAT CCCTAGCCTT ACGTCCACAG 
AGTCCTTGTG AGCGGACGGA TATCAGGATG GCAATAGTAG CCGATCACCT GGGACTTAGT 
TGGACAGAGC TGGCAAGGGA ACTGAATTTT TCAGTGGATG AAATCAACCA AATACGAGTG 
GAAAATCCCA ATTCTTTAAT TTCTCAGAGC TTCATGTTAT TAAAAAAGTG GGTGACGAGA 
GACGGGAAGA ATGCCACAAC TGATGCCTTA ACTTCGGTCT TAACAAAAAT TAACCGAATA 
GACATAGTAA CTCTGCTGGA AGGACCAATA TTTGATTATG GGAATATTTC AGGCACCAGA 
AGCTTTGCAG ATGAAAACAA TGTTTTCCAT GACCCAGTTG ATGGTCACCC TTCCTTTCAA 
GTGGAGCTGG AGACCCCAAT GGGGTTGTAC TGCACACCAC CCACCCCTTT CCAGCAAGAT 
GACCATTTTA GTGATAACTC TAGCATAGAG TCTCCCTTTA GGACCCCCAG TAGACTGAGT 
GACGGGCTGA TGCCTTCCCA GGGAAGCATA GAGCATCCAG CAGGTGGACC TCCAGTGGTA 
ACTGCAGAAG ACACTTCTTT AGAAGACAGC AAAATGGACG ATTCAGTAAC GGTAACAGAA 
ACGGCTGACC CACTGGACGT AGATGAGAGC CAGTTGAAGG ACCTGTGTCA GAGCGAGTGT 
GCTCACTGCT GGGCGAGTGT GCCCGGGGTC CCAAGCGGCG GTCCGCAGGC AGAGCCACTG 
AGAGCGCAGA CTAGAAAAGT AGGCGTGAGC TCTGAACAGC AGGAGAAAGG AGACTCTGGT 
CCTGAGGAGG AGATGGCGGA CGACAAGGTC AGATCTCTGT TTGAGGACAT TCAACTTGAA 
GAAGTAGAGG CTGAGGAGAT GACAGAAGAC GAGGGGCAGG CTATACTTAA CCGTGTTCCG 
CGAGCAGAAC TGGCAATGTC TTCACTTGCA GGTTGGCAGA ACGAGACGCC AAGTGGAAGC 
CTAGAGTCCC CGGCGCAAGC GCGAAGAATA ACCGGTGGGT TACTGGACCG TCTGGACGAC 
AGCTCTGACC AGGTTCGGGA TCCCATTACC TCATACCTCA CAGGAGAAGC TGGGAAGTTC 
GAAGCAAACG GAAACCACGC AGAAGTCATT CCAGAAGCAA AGGCCAAGGC CTACTTTCCT 
GAATCCCAAA ACGATATAGG GAAACAGAGC ATCAAGGAGA ACTTGAAACC AAAAACACAC 
GGATGTGGTC GCGCTGAGGA ACCAGTGTCG CCCCTCACAG CCTACCAGAA ATCTCTAGAA 
GAAACCAGCA AGCTTGTCAT AGAAGACGCA CCTAAACCCT GTGTGCCTGT CGGCATGAAA 
AAGATGACCA GGACTCCGGC TGACGGCAAA GCCAGGCTCA ACCTCCAGGA AGAGGAGGGG 
TCCGCCAGGT CAGAGCCTAA GGACTTGAGA CACAGTGAGA GTGATTCAAG CTCAGAGGAG 
GAGCGGAGGG TCACTACCCG GGTCATACGA CGGCGTGTGA TTATAAAGGG AGAGGAAGCC 
AAAACCATCC CTGGTGAATC TGTCACAGAA GAGCAATTTA CCGATGAAGA AGGCAACCTC 
ATCACTAGAA AAATCACTCG GAAGGTAATA AGACGGATTG GTCCACAAGA GAGGAAGCAG 
GATGATGTGC AGGGAGAAGG CTATAAGGTG AAAACAAAGA AGGAAATCCG GAATGTGGAG 
AAGAAAGCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113993.1
CDS	377..6331
Translation	MSEEAKEKTAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEGEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASPKISSDRSYTLNRSSYARDSMMIEELLVPSKEQHLPFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLSELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVSNGYKGDTTPSLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKVRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFTSLALRPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGHPSFQVELETPMGLYCTPPTPFQQDDHFSDNSSIESPFRTPSRLSDGLMPSQGSIEHPAGGPPVVTAEDTSLEDSKMDDSVTVTETADPLDVDESQLKDLCQSECAHCWASVPGVPSGGPQAEPLRAQTRKVGVSSEQQEKGDSGPEEEMADDKVRSLFEDIQLEEVEAEEMTEDEGQAILNRVPRAELAMSSLAGWQNETPSGSLESPAQARRITGGLLDRLDDSSDQVRDPITSYLTGEAGKFEANGNHAEVIPEAKAKAYFPESQNDIGKQSIKENLKPKTHGCGRAEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTRTPADGKARLNLQEEEGSARSEPKDLRHSESDSSSEEERRVTTRVIRRRVIIKGEEAKTIPGESVTEEQFTDEEGNLITRKITRKVIRRIGPQERKQDDVQGEGYKVKTKKEIRNVEKKAH

Target ORF information:

RefSeq Version	NM_031805.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 3 (Ank3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031805.1

ORF Insert Sequence:

ATGAGCGAAG AGGCAAAGGA GAAAACCGCC AAGCCTGCTC ATAGGAAGAG GAAAGGGAAA 
AAGTCGGATG CCAATGCAAG TTACTTGAGA GCAGCTCGGG CGGGGCACCT GGAAAAGGCC 
CTTGACTACA TCAAAAATGG AGTGGACGTC AACATCTGTA ACCAGAATGG GTTGAATGCG 
CTCCATCTTG CTTCCAAAGA AGGCCATGTG GAAGTGGTCT CCGAGCTGCT GCAGAGAGAA 
GCCAACGTCG ATGCAGCCAC AAAGAAAGGA AACACAGCCT TACACATCGC ATCCTTGGCT 
GGACAAGCGG AAGTGGTCAA GGTCTTGGTT ACGAACGGAG CGAACGTCAA CGCACAGTCT 
CAGAATGGCT TCACGCCGCT GTATATGGCG GCCCAGGAGA ACCACCTGGA AGTCGTCAGG 
TTTCTTCTGG ACAATGGTGC CAGCCAAAGC CTGGCTACAG AGGATGGCTT CACGCCATTG 
GCCGTGGCTC TTCAACAAGG TCACGACCAA GTCGTATCCC TCCTGCTCGA GAACGACACG 
AAGGGAAAAG TGCGCCTCCC AGCCCTCCAC ATCGCGGCCC GGAAAGATGA CACCAAAGCG 
GCAGCTCTGC TCCTACAGAA CGACACCAAT GCAGACATAG AGTCAAAGAG TGGCTTCACC 
CCACTCCACA TAGCTGCCCA CTACGGGAAT ATCAATGTGG CCACGTTGCT GTTAAACCGA 
GCGGCTGCCG TGGACTTCAC CGCACGGAAT GACATCACTC CTTTGCATGT TGCCTCGAAA 
CGGGGAAACG CAAATATGGT AAAGCTGTTG CTCGACCGGG GTGCGAAGAT CGACGCCAAG 
ACCAGGGACG GTCTGACCCC GCTGCACTGC GGGGCGAGAA GTGGCCACGA GCAGGTGGTA 
GAGATGTTGC TGGACAGAGC CGCCCCCATC CTTTCGAAAA CCAAGAATGG ACTGTCCCCA 
CTGCACATGG CCACACAAGG AGACCATTTA AACTGCGTCC AACTCCTCCT CCAGCACAAC 
GTGCCCGTGG ATGACGTCAC CAACGACTAC CTGACCGCCC TCCACGTGGC GGCCCACTGC 
GGCCATTACA AAGTTGCCAA GGTTCTTTTG GATAAGAAAG CTAACCCCAA TGCCAAAGCT 
CTGAATGGCT TCACCCCTCT CCATATCGCC TGCAAAAAGA ACCGAATCCG AGTAATGGAA 
CTCCTTTTGA AGCACGGTGC ATCTATTCAA GCCGTAACCG AGTCGGGCCT TACCCCAATC 
CATGTCGCTG CCTTCATGGG ACATGTAAAT ATCGTGTCAC AGCTAATGCA TCATGGAGCC 
TCCCCAAACA CCACCAACGT GAGAGGAGAA ACAGCATTGC ATATGGCGGC TCGGTCGGGG 
CAAGCAGAAG TGGTGCGGTA TTTGGTACAA GATGGAGCTC AGGTAGAAGC AAAAGCTAAG 
GATGACCAGA CCCCACTCCA CATTTCAGCC CGACTCGGGA AGGCTGACAT AGTGCAACAA 
CTGTTACAGC AAGGAGCGTC CCCGAATGCA GCAACAACTT CCGGGTACAC CCCCCTTCAC 
CTTTCGGCCA GAGAGGGGCA TGAGGATGTA GCTGCCTTCC TTCTGGACCA TGGAGCATCT 
TTATCCATAA CAACAAAGAA GGGATTCACG CCTCTGCACG TGGCTGCAAA ATATGGGAAG 
CTTGAAGTGG CAAGTCTCCT GCTGCAGAAG AGTGCGTCTC CTGATGCCGC TGGGAAGAGC 
GGGCTAACTC CACTGCATGT AGCAGCACAT TACGATAATC AGAAAGTGGC CCTTCTGCTT 
TTGGACCAGG GAGCCTCACC CCACGCAGCT GCAAAGAACG GTTATACGCC ACTGCACATC 
GCCGCCAAGA AGAACCAGAT GGACATAGCC ACGTCCCTGC TGGAGTACGG AGCTGATGCA 
AACGCCGTTA CCCGGCAAGG GATTGCGTCC GTCCACCTTG CAGCGCAGGA AGGGCACGTG 
GACATGGTGT CGCTGCTCCT CAGTAGAAAT GCGAACGTCA ACTTGAGCAA TAAGAGCGGC 
CTCACCCCAC TCCACCTGGC GGCTCAAGAA GACCGAGTGA ACGTGGCTGA GGTCCTTGTC 
AACCAAGGGG CCCACGTGGA TGCACAGACA AAGATGGGCT ACACGCCGCT CCATGTGGGC 
TGTCACTATG GAAACATCAA AATAGTCAAT TTTCTGCTGC AGCATTCTGC GAAAGTTAAT 
GCCAAGACAA AGAATGGATA CACGCCACTG CACCAGGCAG CCCAGCAGGG CCACACACAC 
ATCATCAACG TCCTGCTCCA GAACAACGCT TCCCCCAATG AACTCACTGT GAATGGGAAC 
ACAGCCCTGG CCATCGCCCG ACGCCTTGGC TACATCTCGG TGGTTGACAC CCTAAAGGTC 
GTGACAGAGG AAATTATGAC CACCACTACC ATCACGGAGA AGCACAAAAT GAATGTCCCA 
GAAACGATGA ATGAAGTCCT CGACATGTCA GACGATGAAG GTGAAGATGC CATCACAGGG 
GACACTGACA AGTATCTCGG GCCACAGGAC CTCAAGGAGC TGGGCGATGA CTCCCTGCCT 
GCCGAGGGCT ATGTGGGCTT CAGTCTCGGA GCCCGTTCTG CCAGTCCCAA GATAAGTTCG 
GATAGGTCCT ACACCCTGAA CAGAAGCTCC TATGCGCGGG ACAGCATGAT GATCGAGGAG 
CTTCTGGTGC CATCCAAAGA GCAGCACCTT CCATTCACGA GGGAGTTTGA CTCCGACTCC 
CTCAGACACT ACAGTTGGGC AGCAGACACA CTGGACAATG TGAACCTGGT CTCCAGCCCT 
GTGCATTCTG GGTTTCTGGT TAGCTTTATG GTGGACGCGA GAGGGGGCTC CATGCGAGGA 
AGCCGCCACC ACGGGATGCG GATCATCATC CCTCCGCGCA AGTGTACGGC CCCCACCCGC 
ATCACTTGCC GCCTGGTAAA GAGACATAAA CTGGCCAACC CACCCCCCAT GGTGGAAGGA 
GAGGGATTAG CCAGTAGGCT GGTAGAAATG GGTCCTGCGG GGGCACAATT TTTAGGCCCG 
GTCATAGTGG AAATCCCTCA TTTTGGGTCC ATGAGAGGAA AGGAGAGGGA ACTCATTGTC 
CTCCGGAGCG AGAACGGAGA GACCTGGAAG GAACACCAGT TTGACAGCAA AAACGAAGAC 
CTCTCGGAGC TCCTCAATGG CATGGACGAA GAACTTGACA GCCCAGAAGA GTTGGGCACA 
AAGCGCATCT GCAGAATCAT CACGAAGGAT TTCCCCCAGT ATTTCGCTGT GGTTTCCCGG 
ATTAAGCAGG AAAGCAACCA GATTGGTCCC GAAGGTGGGA TTCTGAGCAG CACCACGGTC 
CCCCTCGTCC AGGCGTCTTT CCCAGAGGGT GCCTTAACCA AAAGGATCCG TGTGGGTCTG 
CAGGCTCAGC CCGTACCAGA GGAGACTGTG AAAAAGATCC TTGGAAACAA AGCAACGTTT 
AGCCCAATTG TCACGGTGGA GCCGAGGAGG AGGAAATTCC ATAAGCCGAT CACCATGACC 
ATCCCGGTGC CCCCACCCTC GGGAGAAGGC GTGTCCAATG GGTACAAGGG GGACACCACG 
CCCAGCCTGC GACTCCTCTG CAGCATTACA GGAGGGACCT CGCCGGCTCA GTGGGAAGAC 
ATCACAGGCA CAACCCCGCT GACCTTCATA AAGGACTGTG TGTCTTTCAC AACCAATGTT 
TCAGCCAGGT TCTGGCTGGC GGACTGCCAT CAGGTGTTAG AAACCGTAGG GCTAGCCTCG 
CAGCTGTACA GAGAGTTGAT ATGTGTCCCC TACATGGCCA AGTTTGTTGT TTTTGCCAAA 
ACAAACGACC CAGTGGAATC CTCCCTAAGG TGCTTCTGTA TGACAGACGA CAGAGTGGAC 
AAAACCCTGG AGCAGCAGGA GAATTTCGAG GAGGTTGCCA GAAGCAAAGA CATCGAGGTT 
CTGGAAGGAA AGCCCATCTA TGTTGATTGC TATGGAAACC TGGCTCCTCT GACCAAAGGA 
GGGCAGCAGC TTGTTTTTAA CTTCTATTCT TTCAAAGAAA ACAGACTGCC ATTTTCCATC 
AAGGTTAGAG ACACCAGCCA AGAGCCCTGC GGCCGTCTGT CTTTCCTGAA GGAGCCGAAA 
ACAACAAAAG GATTACCCCA AACGGCTGTT TGCAACTTAA ATATTACTCT ACCGGCGCAT 
AAAAAGGCTG AGAAGGCAGA CAGACGCCAG AGCTTCACAT CCCTAGCCTT ACGTCCACAG 
AGTCCTTGTG AGCGGACGGA TATCAGGATG GCAATAGTAG CCGATCACCT GGGACTTAGT 
TGGACAGAGC TGGCAAGGGA ACTGAATTTT TCAGTGGATG AAATCAACCA AATACGAGTG 
GAAAATCCCA ATTCTTTAAT TTCTCAGAGC TTCATGTTAT TAAAAAAGTG GGTGACGAGA 
GACGGGAAGA ATGCCACAAC TGATGCCTTA ACTTCGGTCT TAACAAAAAT TAACCGAATA 
GACATAGTAA CTCTGCTGGA AGGACCAATA TTTGATTATG GGAATATTTC AGGCACCAGA 
AGCTTTGCAG ATGAAAACAA TGTTTTCCAT GACCCAGTTG ATGGTCACCC TTCCTTTCAA 
GTGGAGCTGG AGACCCCAAT GGGGTTGTAC TGCACACCAC CCACCCCTTT CCAGCAAGAT 
GACCATTTTA GTGATAACTC TAGCATAGAG TCTCCCTTTA GGACCCCCAG TAGACTGAGT 
GACGGGCTGA TGCCTTCCCA GGGAAGCATA GAGCATCCAG CAGGTGGACC TCCAGTGGTA 
ACTGCAGAAG ACACTTCTTT AGAAGACAGC AAAATGGACG ATTCAGTAAC GGTAACAGAA 
ACGGCTGACC CACTGGACGT AGATGAGAGC CAGTTGAAGG ACCTGTGTCA GAGCGAGTGT 
GCTCACTGCT GGGCGAGTGT GCCCGGGGTC CCAAGCGGCG GTCCGCAGGC AGAGCCACTG 
AGAGCGCAGA CTAGAAAAGT AGGCGTGAGC TCTGAACAGC AGGAGAAAGG AGACTCTGGT 
CCTGAGGAGG AGATGGCGGA CGACAAGGTC AGATCTCTGT TTGAGGACAT TCAACTTGAA 
GAAGTAGAGG CTGAGGAGAT GACAGAAGAC GAGGGGCAGG CTATACTTAA CCGTGTTCCG 
CGAGCAGAAC TGGCAATGTC TTCACTTGCA GGTTGGCAGA ACGAGACGCC AAGTGGAAGC 
CTAGAGTCCC CGGCGCAAGC GCGAAGAATA ACCGGTGGGT TACTGGACCG TCTGGACGAC 
AGCTCTGACC AGGTTCGGGA TCCCATTACC TCATACCTCA CAGGAGAAGC TGGGAAGTTC 
GAAGCAAACG GAAACCACGC AGAAGTCATT CCAGAAGCAA AGGCCAAGGC CTACTTTCCT 
GAATCCCAAA ACGATATAGG GAAACAGAGC ATCAAGGAGA ACTTGAAACC AAAAACACAC 
GGATGTGGTC GCGCTGAGGA ACCAGTGTCG CCCCTCACAG CCTACCAGAA ATCTCTAGAA 
GAAACCAGCA AGCTTGTCAT AGAAGACGCA CCTAAACCCT GTGTGCCTGT CGGCATGAAA 
AAGATGACCA GGACTCCGGC TGACGGCAAA GCCAGGCTCA ACCTCCAGGA AGAGGAGGGG 
TCCGCCAGGT CAGAGCCTAA GGACTTGAGA CACAGTGAGA GTGATTCAAG CTCAGAGGAG 
GAGCGGAGGG TCACTACCCG GGTCATACGA CGGCGTGTGA TTATAAAGGG AGAGGAAGCC 
AAAACCATCC CTGGTGAATC TGTCACAGAA GAGCAATTTA CCGATGAAGA AGGCAACCTC 
ATCACTAGAA AAATCACTCG GAAGGTAATA AGACGGATTG GTCCACAAGA GAGGAAGCAG 
GATGATGTGC AGGGAGAAGG CTATAAGGTG AAAACAAAGA AGGAAATCCG GAATGTGGAG 
AAGAAAGCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ank3 ( NM_001033984.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029156.1 Rattus norvegicus ankyrin 3 (Ank3), transcript variant 2, mRNA.

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CloneID	ORa08809
Clone ID Related Accession (Same CDS sequence)	NM_001033984.1
Accession Version	NM_001033984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559836800000
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF102552.1 and AJ812023.1. Transcript Variant: This variant (2) represents the longest variant and it encodes the longest protein (isoform 2). Isoform 2 is also known as '270 kDa ankyrin-G'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF102552.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCATG CCGCTTCCCA GTTAAAGAAA AACAGGGATT TAGAAATCAA TGCGGAAGAA 
GAGACCGAGA AAAAAAAGAA GCACCGTAAA CGGTCCCGGG ATCGCAAGAA AAAGTCGGAT 
GCCAATGCAA GTTACTTGAG AGCAGCTCGG GCGGGGCACC TGGAAAAGGC CCTTGACTAC 
ATCAAAAATG GAGTGGACGT CAACATCTGT AACCAGAATG GGTTGAATGC GCTCCATCTT 
GCTTCCAAAG AAGGCCATGT GGAAGTGGTC TCCGAGCTGC TGCAGAGAGA AGCCAACGTC 
GATGCAGCCA CAAAGAAAGG AAACACAGCC TTACACATCG CATCCTTGGC TGGACAAGCG 
GAAGTGGTCA AGGTCTTGGT TACGAACGGA GCGAACGTCA ACGCACAGTC TCAGAATGGC 
TTCACGCCGC TGTATATGGC GGCCCAGGAG AACCACCTGG AAGTCGTCAG GTTTCTTCTG 
GACAATGGTG CCAGCCAAAG CCTGGCTACA GAGGATGGCT TCACGCCATT GGCCGTGGCT 
CTTCAACAAG GTCACGACCA AGTCGTATCC CTCCTGCTCG AGAACGACAC GAAGGGAAAA 
GTGCGCCTCC CAGCCCTCCA CATCGCGGCC CGGAAAGATG ACACCAAAGC GGCAGCTCTG 
CTCCTACAGA ACGACACCAA TGCAGACATA GAGTCAAAGA TGGTGGTGAA TAGAGCAACT 
GAGAGTGGCT TCACCCCACT CCACATAGCT GCCCACTACG GGAATATCAA TGTGGCCACG 
TTGCTGTTAA ACCGAGCGGC TGCCGTGGAC TTCACCGCAC GGAATGACAT CACTCCTTTG 
CATGTTGCCT CGAAACGGGG AAACGCAAAT ATGGTAAAGC TGTTGCTCGA CCGGGGTGCG 
AAGATCGACG CCAAGACCAG GGACGGTCTG ACCCCGCTGC ACTGCGGGGC GAGAAGTGGC 
CACGAGCAGG TGGTAGAGAT GTTGCTGGAC AGAGCCGCCC CCATCCTTTC GAAAACCAAG 
AATGGACTGT CCCCACTGCA CATGGCCACA CAAGGAGACC ATTTAAACTG CGTCCAACTC 
CTCCTCCAGC ACAACGTGCC CGTGGATGAC GTCACCAACG ACTACCTGAC CGCCCTCCAC 
GTGGCGGCCC ACTGCGGCCA TTACAAAGTT GCCAAGGTTC TTTTGGATAA GAAAGCTAAC 
CCCAATGCCA AAGCTCTGAA TGGCTTCACC CCTCTCCATA TCGCCTGCAA AAAGAACCGA 
ATCCGAGTAA TGGAACTCCT TTTGAAGCAC GGTGCATCTA TTCAAGCCGT AACCGAGTCG 
GGCCTTACCC CAATCCATGT CGCTGCCTTC ATGGGACATG TAAATATCGT GTCACAGCTA 
ATGCATCATG GAGCCTCCCC AAACACCACC AACGTGAGAG GAGAAACAGC ATTGCATATG 
GCGGCTCGGT CGGGGCAAGC AGAAGTGGTG CGGTATTTGG TACAAGATGG AGCTCAGGTA 
GAAGCAAAAG CTAAGGATGA CCAGACCCCA CTCCACATTT CAGCCCGACT CGGGAAGGCT 
GACATAGTGC AACAACTGTT ACAGCAAGGA GCGTCCCCGA ATGCAGCAAC AACTTCCGGG 
TACACCCCCC TTCACCTTTC GGCCAGAGAG GGGCATGAGG ATGTAGCTGC CTTCCTTCTG 
GACCATGGAG CATCTTTATC CATAACAACA AAGAAGGGAT TCACGCCTCT GCACGTGGCT 
GCAAAATATG GGAAGCTTGA AGTGGCAAGT CTCCTGCTGC AGAAGAGTGC GTCTCCTGAT 
GCCGCTGGGA AGAGCGGGCT AACTCCACTG CATGTAGCAG CACATTACGA TAATCAGAAA 
GTGGCCCTTC TGCTTTTGGA CCAGGGAGCC TCACCCCACG CAGCTGCAAA GAACGGTTAT 
ACGCCACTGC ACATCGCCGC CAAGAAGAAC CAGATGGACA TAGCCACGTC CCTGCTGGAG 
TACGGAGCTG ATGCAAACGC CGTTACCCGG CAAGGGATTG CGTCCGTCCA CCTTGCAGCG 
CAGGAAGGGC ACGTGGACAT GGTGTCGCTG CTCCTCAGTA GAAATGCGAA CGTCAACTTG 
AGCAATAAGA GCGGCCTCAC CCCACTCCAC CTGGCGGCTC AAGAAGACCG AGTGAACGTG 
GCTGAGGTCC TTGTCAACCA AGGGGCCCAC GTGGATGCAC AGACAAAGAT GGGCTACACG 
CCGCTCCATG TGGGCTGTCA CTATGGAAAC ATCAAAATAG TCAATTTTCT GCTGCAGCAT 
TCTGCGAAAG TTAATGCCAA GACAAAGAAT GGATACACGC CACTGCACCA GGCAGCCCAG 
CAGGGCCACA CACACATCAT CAACGTCCTG CTCCAGAACA ACGCTTCCCC CAATGAACTC 
ACTGTGAATG GGAACACAGC CCTGGCCATC GCCCGACGCC TTGGCTACAT CTCGGTGGTT 
GACACCCTAA AGGTCGTGAC AGAGGAAATT ATGACCACCA CTACCATCAC GGAGAAGCAC 
AAAATGAATG TCCCAGAAAC GATGAATGAA GTCCTCGACA TGTCAGACGA TGAAGTTGGT 
AAAGCCAGCG CCCCCGAAAA GCTCAGTGAT GGGGAATATA TCTCAGATGG TGAAGAAGGT 
GAAGATGCCA TCACAGGGGA CACTGACAAG TATCTCGGGC CACAGGACCT CAAGGAGCTG 
GGCGATGACT CCCTGCCTGC CGAGGGCTAT GTGGGCTTCA GTCTCGGAGC CCGTTCTGCC 
AGCCTTCGCT CCTTCAGTTC GGATAGGTCC TACACCCTGA ACAGAAGCTC CTATGCGCGG 
GACAGCATGA TGATCGAGGA GCTTCTGGTG CCATCCAAAG AGCAGCACCT TCCATTCACG 
AGGGAGTTTG ACTCCGACTC CCTCAGACAC TACAGTTGGG CAGCAGACAC ACTGGACAAT 
GTGAACCTGG TCTCCAGCCC TGTGCATTCT GGGTTTCTGG TTAGCTTTAT GGTGGACGCG 
AGAGGGGGCT CCATGCGAGG AAGCCGCCAC CACGGGATGC GGATCATCAT CCCTCCGCGC 
AAGTGTACGG CCCCCACCCG CATCACTTGC CGCCTGGTAA AGAGACATAA ACTGGCCAAC 
CCACCCCCCA TGGTGGAAGG AGAGGGATTA GCCAGTAGGC TGGTAGAAAT GGGTCCTGCG 
GGGGCACAAT TTTTAGGCCC GGTCATAGTG GAAATCCCTC ATTTTGGGTC CATGAGAGGA 
AAGGAGAGGG AACTCATTGT CCTCCGGAGC GAGAACGGAG AGACCTGGAA GGAACACCAG 
TTTGACAGCA AAAACGAAGA CCTCTCGGAG CTCCTCAATG GCATGGACGA AGAACTTGAC 
AGCCCAGAAG AGTTGGGCAC AAAGCGCATC TGCAGAATCA TCACGAAGGA TTTCCCCCAG 
TATTTCGCTG TGGTTTCCCG GATTAAGCAG GAAAGCAACC AGATTGGTCC CGAAGGTGGG 
ATTCTGAGCA GCACCACGGT CCCCCTCGTC CAGGCGTCTT TCCCAGAGGG TGCCTTAACC 
AAAAGGATCC GTGTGGGTCT GCAGGCTCAG CCCGTACCAG AGGAGACTGT GAAAAAGATC 
CTTGGAAACA AAGCAACGTT TAGCCCAATT GTCACGGTGG AGCCGAGGAG GAGGAAATTC 
CATAAGCCGA TCACCATGAC CATCCCGGTG CCCCCACCCT CGGGAGAAGG CGTGTCCAAT 
GGGTACAAGG GGGACACCAC GCCCAGCCTG CGACTCCTCT GCAGCATTAC AGGAGGGACC 
TCGCCGGCTC AGTGGGAAGA CATCACAGGC ACAACCCCGC TGACCTTCAT AAAGGACTGT 
GTGTCTTTCA CAACCAATGT TTCAGCCAGG TTCTGGCTGG CGGACTGCCA TCAGGTGTTA 
GAAACCGTAG GGCTAGCCTC GCAGCTGTAC AGAGAGTTGA TATGTGTCCC CTACATGGCC 
AAGTTTGTTG TTTTTGCCAA AACAAACGAC CCAGTGGAAT CCTCCCTAAG GTGCTTCTGT 
ATGACAGACG ACAGAGTGGA CAAAACCCTG GAGCAGCAGG AGAATTTCGA GGAGGTTGCC 
AGAAGCAAAG ACATCGAGGT TCTGGAAGGA AAGCCCATCT ATGTTGATTG CTATGGAAAC 
CTGGCTCCTC TGACCAAAGG AGGGCAGCAG CTTGTTTTTA ACTTCTATTC TTTCAAAGAA 
AACAGACTGC CATTTTCCAT CAAGGTTAGA GACACCAGCC AAGAGCCCTG CGGCCGTCTG 
TCTTTCCTGA AGGAGCCGAA AACAACAAAA GGATTACCCC AAACGGCTGT TTGCAACTTA 
AATATTACTC TACCGGCGCA TAAAAAGGCT GAGAAGGCAG ACAGACGCCA GAGCTTCACA 
TCCCTAGCCT TACGTAAGCG CTACAGCTAC TTGACTGAGC CCAGCATGAA AACAGTTGAA 
CGGAGTTCAG GAACAGCAAG ATCCCTTCCC ACTACTTATT CACACAAGCC ATTCTTTTCT 
ACAAGACCAT ACCAGTCCTG GACTACGACT CCCATAACAG TGCCCGGGCC GGCCAAATCA 
GGCTCCTTAT CCAGCTCCCC CTCTAACACG CCGTCAGCGT CTCCGTTAAA ATCCATATGG 
TCTGTTTCAA CGCCTTCTCC GATCAAATCC ACGCTAGGCG CCTCAACTAC GTCATCAGTC 
AAATCCATTA GTGATGTGGC GTCTCCTATT AGATCTTTCC GGACAATTTC TTCGCCAATA 
AGAACGGTGG CATCGCCGTC TCCATACAAT ACCCAAGTAG CCTCTGGTAC CCTTGGGAGG 
GTACCCACCA TCACAGAGGC CACACCTATA AAGGGGGTGG CTCCCAACTC AACTTTGTCC 
TCTCGAACTT CTCCTGTGAC GACCGCAGGG TCTCTGTTGG AGAAGTCGTC CATCACCATG 
ACACCCCCTG CCTCCCCCAA AGCCAACATC ACCATGTATT CCTCCAGCTT GCCATTTAAG 
TCCATTATCA CGTCAGCGGC ACCGCTGATC TCCTCCCCTT TAAAGTCAGT GGTGTCTCCG 
ACCAAGTCTG CAGCTGATGT CATCTCAACA GCTAAAGCTG CGATGGCATC GACTCTCTCC 
TCCCCCTTAA AGCAGATGTC TGGACATGCA GAAGTAGCGC TAGTCAATGG GTCTGTTTCT 
CCTCTGAAGT ATCCTTCCTC TTCCGCTTTA ATTAACGGAT GCAAAGCCAC TGCCACATTA 
CAGGACAAAA TTTCTACAGC CACAAATGCT GTGAGCTCGG TGGTGAGCGC GGCCCCTGAC 
ACGGTGGAGA AAGCGCTCTC CACCACAACA GCCATGCCCT TTTCCCCACT CAGGTCTTAC 
GTTTCCGCTG CAGCGCCCTC TGCTTTTCAG TCCCTTAGAG CTCCCTCTGC CAGTGCACTC 
TACAACTCCC TCGGGCCTTC AGTAGGTGTT ACTACCTCAT CCGTAACTTC ATCGATAATC 
ACAGTGCCAG TATACTCGGT AGGCAATGTT TTGGCAGAAC CAGCATTGAA GAAACTTCCA 
GACTCTAACT CGCTCACAAA ATCGGCAGCG GCTTTGCTGT CGCCCATTAA AACATTGACT 
ACGGAGACAC GTCCTCAGCC CCATTTCAAT CGAACGTCAT CTCCCGTCAA GTCCTCTCTG 
TTCCTGGCAT CCTCGGCCCT TAAGCCGTCA GTACCATCTT CTTTATCTTC CAGTCAGGAG 
ATCTTAAAAG ATGTGGCCGA GATGAAGGAG GACCTCATGA GAATGACCGC CATACTGCAG 
ACGGATGTGC CTGAGGAGAA GCCATTCCAG ACCGACCTCC CCCGAGAAGG GAGGATAGAC 
GACGAAGAGC CTTTCAAAAT CGTTGAGAAA GTGAAGGAAG ACTTAGTCAA AGTCAGTGAG 
ATACTCAAAA AGGACGTGTG TGTCGAGAGC AAAGGGCCAC CCAAGTCCCC AAAGAGTGAC 
AAAGGACACT CTCCAGAAGA TGACTGGACG GAATTTAGTT CTGAGGAAAT ACGAGAAGCC 
AGGCAGGCTG CTGCCAGTCA CGCCCCATCC CTGCCCGAGA GAGTACATGG AAAGGCCAAC 
CTCACCAGAG TCATAGACTA CCTGACCAAT GACATCGGGA GCAGTTCACT GACCAACTTA 
AAGTACAAGT TTGAAGAGGC TAAGAAGGAA GGAGAGGAGA GACAGAAGAG AATTTTAAAG 
CCAGCCATGG CCTTGCAGGA GCATAAACTC AAAATGCCGC CAGCCTCCAT GAGACCTTCC 
ACCTCCGAGA AGGAACTGTG CAAGATGGCG GACTCCTTTT TTGGAACAGA TGCCATCCTA 
GAGTCTCCCG ATGACTTTTC TCAACATGAC CAAGACAAAA GTCCCTTGTC CGACAGTGGC 
TTTGAAACCC GAAGTGAGAA AACACCTTCA GCCCCCCAAA GTGCCGAGAG CACCGGCCCT 
AAGCCACTAT TTCATGAAGT CCCCATCCCC CCTGTCATCA CAGAGACGAG AACTGAAGTA 
GTCCACGTGA TCAGGAGCTA CGAGCCCTCT ACTGGGGAGA TCCCCCAGTC CCAGCCAGAG 
GACCCCGTGT CTCCCAAACC TCCCCCTACA TTTATGGAGT TGGAACCAAA GCCCACCGCC 
CTTAGCATTA AGGAAAAAGT GAAAGCATTC CAAATGAAAG CCAGTAGCGA GGAAGAGGAC 
CACAGTCGGG TTTTAAGCAA AGGCATGCGT GTCAAAGAAG AGACTCACAT CACCACAACC 
ACGAGGATGG TGTATCACTC CCCGCCTGGC AGTGAGTGTG CCTCCGAGAG GATTGAGGAG 
ACCATGTCAG TCCATGACAT TATGAAGGCT TTTCAGTCCG GGCGAGACCC TTCCAAAGAG 
CTGGCGGGGC TGTTTGAACA CAAGTCGGCC ATGTCTCCGG ATGTTGCCAA GTCTGCTGCT 
GAGACCTCGG CCCAGCACGC AGAGAAGGAC AACCAAATGA AACCCAAACT GGAGCGTATT 
ATAGAGGTCC ACATCGAAAA AGGTCCACAG AGTCCTTGTG AGCGGACGGA TATCAGGATG 
GCAATAGTAG CCGATCACCT GGGACTTAGT TGGACAGAGC TGGCAAGGGA ACTGAATTTT 
TCAGTGGATG AAATCAACCA AATACGAGTG GAAAATCCCA ATTCTTTAAT TTCTCAGAGC 
TTCATGTTAT TAAAAAAGTG GGTGACGAGA GACGGGAAGA ATGCCACAAC TGATGCCTTA 
ACTTCGGTCT TAACAAAAAT TAACCGAATA GACATAGTAA CTCTGCTGGA AGGACCAATA 
TTTGATTATG GGAATATTTC AGGCACCAGA AGCTTTGCAG ATGAAAACAA TGTTTTCCAT 
GACCCAGTTG ATGGTTGGCA GAACGAGACG CCAAGTGGAA GCCTAGAGTC CCCGGCGCAA 
GCGCGAAGAA TAACCGGTGG GTTACTGGAC CGTCTGGACG ACAGCTCTGA CCAGGTTCGG 
GATCCCATTA CCTCATACCT CACAGGAGAA GCTGGGAAGT TCGAAGCAAA CGGAAACCAC 
GCAGAAGTCA TTCCAGAAGC AAAGGCCAAG GCCTACTTTC CTGAATCCCA AAACGATATA 
GGGAAACAGA GCATCAAGGA GAACTTGAAA CCAAAAACAC ACGGATGTGG TCGCGCTGAG 
GAACCAGTGT CGCCCCTCAC AGCCTACCAG AAATCTCTAG AAGAAACCAG CAAGCTTGTC 
ATAGAAGACG CACCTAAACC CTGTGTGCCT GTCGGCATGA AAAAGATGAC CAGGACTCCG 
GCTGACGGCA AAGCCAGGCT CAACCTCCAG GAAGAGGAGG GGTCCGCCAG GTCAGAGCCT 
AAGCAGGGAG AAGGCTATAA GGTGAAAACA AAGAAGGAAA TCCGGAATGT GGAGAAGAAA 
GCCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029156.1
CDS	1..7869
Translation	MAHAASQLKKNRDLEINAEEETEKKKKHRKRSRDRKKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEVGKASAPEKLSDGEYISDGEEGEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQHLPFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLSELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEGVSNGYKGDTTPSLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKVRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFTSLALRKRYSYLTEPSMKTVERSSGTARSLPTTYSHKPFFSTRPYQSWTTTPITVPGPAKSGSLSSSPSNTPSASPLKSIWSVSTPSPIKSTLGASTTSSVKSISDVASPIRSFRTISSPIRTVASPSPYNTQVASGTLGRVPTITEATPIKGVAPNSTLSSRTSPVTTAGSLLEKSSITMTPPASPKANITMYSSSLPFKSIITSAAPLISSPLKSVVSPTKSAADVISTAKAAMASTLSSPLKQMSGHAEVALVNGSVSPLKYPSSSALINGCKATATLQDKISTATNAVSSVVSAAPDTVEKALSTTTAMPFSPLRSYVSAAAPSAFQSLRAPSASALYNSLGPSVGVTTSSVTSSIITVPVYSVGNVLAEPALKKLPDSNSLTKSAAALLSPIKTLTTETRPQPHFNRTSSPVKSSLFLASSALKPSVPSSLSSSQEILKDVAEMKEDLMRMTAILQTDVPEEKPFQTDLPREGRIDDEEPFKIVEKVKEDLVKVSEILKKDVCVESKGPPKSPKSDKGHSPEDDWTEFSSEEIREARQAAASHAPSLPERVHGKANLTRVIDYLTNDIGSSSLTNLKYKFEEAKKEGEERQKRILKPAMALQEHKLKMPPASMRPSTSEKELCKMADSFFGTDAILESPDDFSQHDQDKSPLSDSGFETRSEKTPSAPQSAESTGPKPLFHEVPIPPVITETRTEVVHVIRSYEPSTGEIPQSQPEDPVSPKPPPTFMELEPKPTALSIKEKVKAFQMKASSEEEDHSRVLSKGMRVKEETHITTTTRMVYHSPPGSECASERIEETMSVHDIMKAFQSGRDPSKELAGLFEHKSAMSPDVAKSAAETSAQHAEKDNQMKPKLERIIEVHIEKGPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGWQNETPSGSLESPAQARRITGGLLDRLDDSSDQVRDPITSYLTGEAGKFEANGNHAEVIPEAKAKAYFPESQNDIGKQSIKENLKPKTHGCGRAEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTRTPADGKARLNLQEEEGSARSEPKQGEGYKVKTKKEIRNVEKKAH

Target ORF information:

RefSeq Version	NM_001033984.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin 3 (Ank3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033984.1

ORF Insert Sequence:

ATGGCTCATG CCGCTTCCCA GTTAAAGAAA AACAGGGATT TAGAAATCAA TGCGGAAGAA 
GAGACCGAGA AAAAAAAGAA GCACCGTAAA CGGTCCCGGG ATCGCAAGAA AAAGTCGGAT 
GCCAATGCAA GTTACTTGAG AGCAGCTCGG GCGGGGCACC TGGAAAAGGC CCTTGACTAC 
ATCAAAAATG GAGTGGACGT CAACATCTGT AACCAGAATG GGTTGAATGC GCTCCATCTT 
GCTTCCAAAG AAGGCCATGT GGAAGTGGTC TCCGAGCTGC TGCAGAGAGA AGCCAACGTC 
GATGCAGCCA CAAAGAAAGG AAACACAGCC TTACACATCG CATCCTTGGC TGGACAAGCG 
GAAGTGGTCA AGGTCTTGGT TACGAACGGA GCGAACGTCA ACGCACAGTC TCAGAATGGC 
TTCACGCCGC TGTATATGGC GGCCCAGGAG AACCACCTGG AAGTCGTCAG GTTTCTTCTG 
GACAATGGTG CCAGCCAAAG CCTGGCTACA GAGGATGGCT TCACGCCATT GGCCGTGGCT 
CTTCAACAAG GTCACGACCA AGTCGTATCC CTCCTGCTCG AGAACGACAC GAAGGGAAAA 
GTGCGCCTCC CAGCCCTCCA CATCGCGGCC CGGAAAGATG ACACCAAAGC GGCAGCTCTG 
CTCCTACAGA ACGACACCAA TGCAGACATA GAGTCAAAGA TGGTGGTGAA TAGAGCAACT 
GAGAGTGGCT TCACCCCACT CCACATAGCT GCCCACTACG GGAATATCAA TGTGGCCACG 
TTGCTGTTAA ACCGAGCGGC TGCCGTGGAC TTCACCGCAC GGAATGACAT CACTCCTTTG 
CATGTTGCCT CGAAACGGGG AAACGCAAAT ATGGTAAAGC TGTTGCTCGA CCGGGGTGCG 
AAGATCGACG CCAAGACCAG GGACGGTCTG ACCCCGCTGC ACTGCGGGGC GAGAAGTGGC 
CACGAGCAGG TGGTAGAGAT GTTGCTGGAC AGAGCCGCCC CCATCCTTTC GAAAACCAAG 
AATGGACTGT CCCCACTGCA CATGGCCACA CAAGGAGACC ATTTAAACTG CGTCCAACTC 
CTCCTCCAGC ACAACGTGCC CGTGGATGAC GTCACCAACG ACTACCTGAC CGCCCTCCAC 
GTGGCGGCCC ACTGCGGCCA TTACAAAGTT GCCAAGGTTC TTTTGGATAA GAAAGCTAAC 
CCCAATGCCA AAGCTCTGAA TGGCTTCACC CCTCTCCATA TCGCCTGCAA AAAGAACCGA 
ATCCGAGTAA TGGAACTCCT TTTGAAGCAC GGTGCATCTA TTCAAGCCGT AACCGAGTCG 
GGCCTTACCC CAATCCATGT CGCTGCCTTC ATGGGACATG TAAATATCGT GTCACAGCTA 
ATGCATCATG GAGCCTCCCC AAACACCACC AACGTGAGAG GAGAAACAGC ATTGCATATG 
GCGGCTCGGT CGGGGCAAGC AGAAGTGGTG CGGTATTTGG TACAAGATGG AGCTCAGGTA 
GAAGCAAAAG CTAAGGATGA CCAGACCCCA CTCCACATTT CAGCCCGACT CGGGAAGGCT 
GACATAGTGC AACAACTGTT ACAGCAAGGA GCGTCCCCGA ATGCAGCAAC AACTTCCGGG 
TACACCCCCC TTCACCTTTC GGCCAGAGAG GGGCATGAGG ATGTAGCTGC CTTCCTTCTG 
GACCATGGAG CATCTTTATC CATAACAACA AAGAAGGGAT TCACGCCTCT GCACGTGGCT 
GCAAAATATG GGAAGCTTGA AGTGGCAAGT CTCCTGCTGC AGAAGAGTGC GTCTCCTGAT 
GCCGCTGGGA AGAGCGGGCT AACTCCACTG CATGTAGCAG CACATTACGA TAATCAGAAA 
GTGGCCCTTC TGCTTTTGGA CCAGGGAGCC TCACCCCACG CAGCTGCAAA GAACGGTTAT 
ACGCCACTGC ACATCGCCGC CAAGAAGAAC CAGATGGACA TAGCCACGTC CCTGCTGGAG 
TACGGAGCTG ATGCAAACGC CGTTACCCGG CAAGGGATTG CGTCCGTCCA CCTTGCAGCG 
CAGGAAGGGC ACGTGGACAT GGTGTCGCTG CTCCTCAGTA GAAATGCGAA CGTCAACTTG 
AGCAATAAGA GCGGCCTCAC CCCACTCCAC CTGGCGGCTC AAGAAGACCG AGTGAACGTG 
GCTGAGGTCC TTGTCAACCA AGGGGCCCAC GTGGATGCAC AGACAAAGAT GGGCTACACG 
CCGCTCCATG TGGGCTGTCA CTATGGAAAC ATCAAAATAG TCAATTTTCT GCTGCAGCAT 
TCTGCGAAAG TTAATGCCAA GACAAAGAAT GGATACACGC CACTGCACCA GGCAGCCCAG 
CAGGGCCACA CACACATCAT CAACGTCCTG CTCCAGAACA ACGCTTCCCC CAATGAACTC 
ACTGTGAATG GGAACACAGC CCTGGCCATC GCCCGACGCC TTGGCTACAT CTCGGTGGTT 
GACACCCTAA AGGTCGTGAC AGAGGAAATT ATGACCACCA CTACCATCAC GGAGAAGCAC 
AAAATGAATG TCCCAGAAAC GATGAATGAA GTCCTCGACA TGTCAGACGA TGAAGTTGGT 
AAAGCCAGCG CCCCCGAAAA GCTCAGTGAT GGGGAATATA TCTCAGATGG TGAAGAAGGT 
GAAGATGCCA TCACAGGGGA CACTGACAAG TATCTCGGGC CACAGGACCT CAAGGAGCTG 
GGCGATGACT CCCTGCCTGC CGAGGGCTAT GTGGGCTTCA GTCTCGGAGC CCGTTCTGCC 
AGCCTTCGCT CCTTCAGTTC GGATAGGTCC TACACCCTGA ACAGAAGCTC CTATGCGCGG 
GACAGCATGA TGATCGAGGA GCTTCTGGTG CCATCCAAAG AGCAGCACCT TCCATTCACG 
AGGGAGTTTG ACTCCGACTC CCTCAGACAC TACAGTTGGG CAGCAGACAC ACTGGACAAT 
GTGAACCTGG TCTCCAGCCC TGTGCATTCT GGGTTTCTGG TTAGCTTTAT GGTGGACGCG 
AGAGGGGGCT CCATGCGAGG AAGCCGCCAC CACGGGATGC GGATCATCAT CCCTCCGCGC 
AAGTGTACGG CCCCCACCCG CATCACTTGC CGCCTGGTAA AGAGACATAA ACTGGCCAAC 
CCACCCCCCA TGGTGGAAGG AGAGGGATTA GCCAGTAGGC TGGTAGAAAT GGGTCCTGCG 
GGGGCACAAT TTTTAGGCCC GGTCATAGTG GAAATCCCTC ATTTTGGGTC CATGAGAGGA 
AAGGAGAGGG AACTCATTGT CCTCCGGAGC GAGAACGGAG AGACCTGGAA GGAACACCAG 
TTTGACAGCA AAAACGAAGA CCTCTCGGAG CTCCTCAATG GCATGGACGA AGAACTTGAC 
AGCCCAGAAG AGTTGGGCAC AAAGCGCATC TGCAGAATCA TCACGAAGGA TTTCCCCCAG 
TATTTCGCTG TGGTTTCCCG GATTAAGCAG GAAAGCAACC AGATTGGTCC CGAAGGTGGG 
ATTCTGAGCA GCACCACGGT CCCCCTCGTC CAGGCGTCTT TCCCAGAGGG TGCCTTAACC 
AAAAGGATCC GTGTGGGTCT GCAGGCTCAG CCCGTACCAG AGGAGACTGT GAAAAAGATC 
CTTGGAAACA AAGCAACGTT TAGCCCAATT GTCACGGTGG AGCCGAGGAG GAGGAAATTC 
CATAAGCCGA TCACCATGAC CATCCCGGTG CCCCCACCCT CGGGAGAAGG CGTGTCCAAT 
GGGTACAAGG GGGACACCAC GCCCAGCCTG CGACTCCTCT GCAGCATTAC AGGAGGGACC 
TCGCCGGCTC AGTGGGAAGA CATCACAGGC ACAACCCCGC TGACCTTCAT AAAGGACTGT 
GTGTCTTTCA CAACCAATGT TTCAGCCAGG TTCTGGCTGG CGGACTGCCA TCAGGTGTTA 
GAAACCGTAG GGCTAGCCTC GCAGCTGTAC AGAGAGTTGA TATGTGTCCC CTACATGGCC 
AAGTTTGTTG TTTTTGCCAA AACAAACGAC CCAGTGGAAT CCTCCCTAAG GTGCTTCTGT 
ATGACAGACG ACAGAGTGGA CAAAACCCTG GAGCAGCAGG AGAATTTCGA GGAGGTTGCC 
AGAAGCAAAG ACATCGAGGT TCTGGAAGGA AAGCCCATCT ATGTTGATTG CTATGGAAAC 
CTGGCTCCTC TGACCAAAGG AGGGCAGCAG CTTGTTTTTA ACTTCTATTC TTTCAAAGAA 
AACAGACTGC CATTTTCCAT CAAGGTTAGA GACACCAGCC AAGAGCCCTG CGGCCGTCTG 
TCTTTCCTGA AGGAGCCGAA AACAACAAAA GGATTACCCC AAACGGCTGT TTGCAACTTA 
AATATTACTC TACCGGCGCA TAAAAAGGCT GAGAAGGCAG ACAGACGCCA GAGCTTCACA 
TCCCTAGCCT TACGTAAGCG CTACAGCTAC TTGACTGAGC CCAGCATGAA AACAGTTGAA 
CGGAGTTCAG GAACAGCAAG ATCCCTTCCC ACTACTTATT CACACAAGCC ATTCTTTTCT 
ACAAGACCAT ACCAGTCCTG GACTACGACT CCCATAACAG TGCCCGGGCC GGCCAAATCA 
GGCTCCTTAT CCAGCTCCCC CTCTAACACG CCGTCAGCGT CTCCGTTAAA ATCCATATGG 
TCTGTTTCAA CGCCTTCTCC GATCAAATCC ACGCTAGGCG CCTCAACTAC GTCATCAGTC 
AAATCCATTA GTGATGTGGC GTCTCCTATT AGATCTTTCC GGACAATTTC TTCGCCAATA 
AGAACGGTGG CATCGCCGTC TCCATACAAT ACCCAAGTAG CCTCTGGTAC CCTTGGGAGG 
GTACCCACCA TCACAGAGGC CACACCTATA AAGGGGGTGG CTCCCAACTC AACTTTGTCC 
TCTCGAACTT CTCCTGTGAC GACCGCAGGG TCTCTGTTGG AGAAGTCGTC CATCACCATG 
ACACCCCCTG CCTCCCCCAA AGCCAACATC ACCATGTATT CCTCCAGCTT GCCATTTAAG 
TCCATTATCA CGTCAGCGGC ACCGCTGATC TCCTCCCCTT TAAAGTCAGT GGTGTCTCCG 
ACCAAGTCTG CAGCTGATGT CATCTCAACA GCTAAAGCTG CGATGGCATC GACTCTCTCC 
TCCCCCTTAA AGCAGATGTC TGGACATGCA GAAGTAGCGC TAGTCAATGG GTCTGTTTCT 
CCTCTGAAGT ATCCTTCCTC TTCCGCTTTA ATTAACGGAT GCAAAGCCAC TGCCACATTA 
CAGGACAAAA TTTCTACAGC CACAAATGCT GTGAGCTCGG TGGTGAGCGC GGCCCCTGAC 
ACGGTGGAGA AAGCGCTCTC CACCACAACA GCCATGCCCT TTTCCCCACT CAGGTCTTAC 
GTTTCCGCTG CAGCGCCCTC TGCTTTTCAG TCCCTTAGAG CTCCCTCTGC CAGTGCACTC 
TACAACTCCC TCGGGCCTTC AGTAGGTGTT ACTACCTCAT CCGTAACTTC ATCGATAATC 
ACAGTGCCAG TATACTCGGT AGGCAATGTT TTGGCAGAAC CAGCATTGAA GAAACTTCCA 
GACTCTAACT CGCTCACAAA ATCGGCAGCG GCTTTGCTGT CGCCCATTAA AACATTGACT 
ACGGAGACAC GTCCTCAGCC CCATTTCAAT CGAACGTCAT CTCCCGTCAA GTCCTCTCTG 
TTCCTGGCAT CCTCGGCCCT TAAGCCGTCA GTACCATCTT CTTTATCTTC CAGTCAGGAG 
ATCTTAAAAG ATGTGGCCGA GATGAAGGAG GACCTCATGA GAATGACCGC CATACTGCAG 
ACGGATGTGC CTGAGGAGAA GCCATTCCAG ACCGACCTCC CCCGAGAAGG GAGGATAGAC 
GACGAAGAGC CTTTCAAAAT CGTTGAGAAA GTGAAGGAAG ACTTAGTCAA AGTCAGTGAG 
ATACTCAAAA AGGACGTGTG TGTCGAGAGC AAAGGGCCAC CCAAGTCCCC AAAGAGTGAC 
AAAGGACACT CTCCAGAAGA TGACTGGACG GAATTTAGTT CTGAGGAAAT ACGAGAAGCC 
AGGCAGGCTG CTGCCAGTCA CGCCCCATCC CTGCCCGAGA GAGTACATGG AAAGGCCAAC 
CTCACCAGAG TCATAGACTA CCTGACCAAT GACATCGGGA GCAGTTCACT GACCAACTTA 
AAGTACAAGT TTGAAGAGGC TAAGAAGGAA GGAGAGGAGA GACAGAAGAG AATTTTAAAG 
CCAGCCATGG CCTTGCAGGA GCATAAACTC AAAATGCCGC CAGCCTCCAT GAGACCTTCC 
ACCTCCGAGA AGGAACTGTG CAAGATGGCG GACTCCTTTT TTGGAACAGA TGCCATCCTA 
GAGTCTCCCG ATGACTTTTC TCAACATGAC CAAGACAAAA GTCCCTTGTC CGACAGTGGC 
TTTGAAACCC GAAGTGAGAA AACACCTTCA GCCCCCCAAA GTGCCGAGAG CACCGGCCCT 
AAGCCACTAT TTCATGAAGT CCCCATCCCC CCTGTCATCA CAGAGACGAG AACTGAAGTA 
GTCCACGTGA TCAGGAGCTA CGAGCCCTCT ACTGGGGAGA TCCCCCAGTC CCAGCCAGAG 
GACCCCGTGT CTCCCAAACC TCCCCCTACA TTTATGGAGT TGGAACCAAA GCCCACCGCC 
CTTAGCATTA AGGAAAAAGT GAAAGCATTC CAAATGAAAG CCAGTAGCGA GGAAGAGGAC 
CACAGTCGGG TTTTAAGCAA AGGCATGCGT GTCAAAGAAG AGACTCACAT CACCACAACC 
ACGAGGATGG TGTATCACTC CCCGCCTGGC AGTGAGTGTG CCTCCGAGAG GATTGAGGAG 
ACCATGTCAG TCCATGACAT TATGAAGGCT TTTCAGTCCG GGCGAGACCC TTCCAAAGAG 
CTGGCGGGGC TGTTTGAACA CAAGTCGGCC ATGTCTCCGG ATGTTGCCAA GTCTGCTGCT 
GAGACCTCGG CCCAGCACGC AGAGAAGGAC AACCAAATGA AACCCAAACT GGAGCGTATT 
ATAGAGGTCC ACATCGAAAA AGGTCCACAG AGTCCTTGTG AGCGGACGGA TATCAGGATG 
GCAATAGTAG CCGATCACCT GGGACTTAGT TGGACAGAGC TGGCAAGGGA ACTGAATTTT 
TCAGTGGATG AAATCAACCA AATACGAGTG GAAAATCCCA ATTCTTTAAT TTCTCAGAGC 
TTCATGTTAT TAAAAAAGTG GGTGACGAGA GACGGGAAGA ATGCCACAAC TGATGCCTTA 
ACTTCGGTCT TAACAAAAAT TAACCGAATA GACATAGTAA CTCTGCTGGA AGGACCAATA 
TTTGATTATG GGAATATTTC AGGCACCAGA AGCTTTGCAG ATGAAAACAA TGTTTTCCAT 
GACCCAGTTG ATGGTTGGCA GAACGAGACG CCAAGTGGAA GCCTAGAGTC CCCGGCGCAA 
GCGCGAAGAA TAACCGGTGG GTTACTGGAC CGTCTGGACG ACAGCTCTGA CCAGGTTCGG 
GATCCCATTA CCTCATACCT CACAGGAGAA GCTGGGAAGT TCGAAGCAAA CGGAAACCAC 
GCAGAAGTCA TTCCAGAAGC AAAGGCCAAG GCCTACTTTC CTGAATCCCA AAACGATATA 
GGGAAACAGA GCATCAAGGA GAACTTGAAA CCAAAAACAC ACGGATGTGG TCGCGCTGAG 
GAACCAGTGT CGCCCCTCAC AGCCTACCAG AAATCTCTAG AAGAAACCAG CAAGCTTGTC 
ATAGAAGACG CACCTAAACC CTGTGTGCCT GTCGGCATGA AAAAGATGAC CAGGACTCCG 
GCTGACGGCA AAGCCAGGCT CAACCTCCAG GAAGAGGAGG GGTCCGCCAG GTCAGAGCCT 
AAGCAGGGAG AAGGCTATAA GGTGAAAACA AAGAAGGAAA TCCGGAATGT GGAGAAGAAA 
GCCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autoinhibition of ankyrin-B/G membrane target bindings by intrinsically disordered segments from the tail regions.
eLife6(2017 08)
Chen K,Li J,Wang C,Wei Z,Zhang M

MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons.
Neuron94(2)347-362.e7(2017 Apr)
Gumy LF,Katrukha EA,Grigoriev I,Jaarsma D,Kapitein LC,Akhmanova A,Hoogenraad CC

Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.
Molecular neurodegeneration11(1)47(2016 06)
Sohn PD,Tracy TE,Son HI,Zhou Y,Leite RE,Miller BL,Seeley WW,Grinberg LT,Gan L

Structural basis for the membrane association of ankyrinG via palmitoylation.
Scientific reports623981(2016 Apr)
Fujiwara Y,Kondo HX,Shirota M,Kobayashi M,Takeshita K,Nakagawa A,Okamura Y,Kinoshita K

TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.
Neuron88(6)1208-1226(2015 Dec)
van Beuningen SFB,Will L,Harterink M,Chazeau A,van Battum EY,Frias CP,Franker MAM,Katrukha EA,Stucchi R,Vocking K,Antunes AT,Slenders L,Doulkeridou S,Sillevis Smitt P,Altelaar AFM,Post JA,Akhmanova A,Pasterkamp RJ,Kapitein LC,de Graaff E,Hoogenraad CC

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Ank3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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