Sufu cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sufu
Entrez Gene ID	361769
Full Name	SUFU negative regulator of hedgehog signaling
General protein information	Preferred Names SUFU negative regulator of hedgehog signaling Names suppressor of fused homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024899.1	NP_001020070.1	suppressor of fused homolog
XM_008760443.2	XP_008758665.1	suppressor of fused homolog isoform X2
XM_006231546.3	XP_006231608.1	suppressor of fused homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SUFU	NP_057253.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001948	XP_321114.1
Pan troglodytes (chimpanzee)	SUFU	XP_001171637.1
Bos taurus (cattle)	SUFU	NP_001091552.1
Rattus norvegicus (Norway rat)	Sufu	NP_001020070.1
Danio rerio (zebrafish)	sufu	NP_958466.1
Mus musculus (house mouse)	Sufu	NP_056567.2
Gallus gallus (chicken)	SUFU	NP_989595.1
Macaca mulatta (Rhesus monkey)	SUFU	XP_001113024.1
Canis lupus familiaris (dog)	SUFU	XP_005637731.1
Drosophila melanogaster (fruit fly)	Su(fu)	NP_536750.2
Xenopus tropicalis (tropical clawed frog)	sufu	NP_001123387.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-874 targeting SUFU involves in osteoblast proliferation and differentiation in osteoporosis rats through the Hedgehog signaling pathway.
Lin JC, Liu ZG, Yu B, Zhang XR
Biochemical and biophysical research communications506(1)194-203(2018 11)

Hypomorphic Recessive Variants in SUFU Impair the Sonic Hedgehog Pathway and Cause Joubert Syndrome with Cranio-facial and Skeletal Defects.
De Mori R, Romani M, D'Arrigo S, Zaki MS, Lorefice E, Tardivo S, Biagini T, Stanley V, Musaev D, Fluss J, Micalizzi A, Nuovo S, Illi B, Chiapparini L, Di Marcotullio L, Issa MY, Anello D, Casella A, Ginevrino M, Leggins AS, Roosing S, Alfonsi R, Rosati J, Schot R, Mancini GMS, Bertini E, Dobyns WB, Mazza T, Gleeson JG, Valente EM
American journal of human genetics101(4)552-563(2017 Oct)

SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development.
Raducu M, Fung E, Serres S, Infante P, Barberis A, Fischer R, Bristow C, Thézénas ML, Finta C, Christianson JC, Buffa FM, Kessler BM, Sibson NR, Di Marcotullio L, Toftgård R, D'Angiolella V
The EMBO journal35(13)1400-16(2016 07)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Modulation of the transactivation function and stability of Krüppel-like zinc finger protein Gli-similar 3 (Glis3) by Suppressor of Fused.
ZeRuth GT, Yang XP, Jetten AM
The Journal of biological chemistry286(25)22077-89(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sufu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sufu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06823	NM_001024899.1 Latest version!	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa24202	XM_006231546.3 Latest version!	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa48010	XM_008760443.2 Latest version!	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sufu ( NM_001024899.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020070.1 Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), mRNA.

Price & Availability↑

CloneID	ORa06823
Clone ID Related Accession (Same CDS sequence)	NM_001024899.1
Accession Version	NM_001024899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1558886400000
Organism	Rattus norvegicus(Norway rat)
Product	suppressor of fused homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097447.1. On Jun 17, 2005 this sequence version replaced XM_342061.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGAGGCCTAG CGTCGCCCCC GGCCCCGCCG CGCCCCCGGC CTCTGGCCCT 
AGTGCCCCCC CGGCCTTTGC TTCACTCTTT CCCCCGGGAC TCCACGCCAT CTACGGAGAG 
TGCCGCCGCC TCTACCCTGA CCAGCCGAAC CCGCTCCAGG TTACCGCTAT CGTCAAGTAC 
TGGTTGGGTG GTCCAGACCC CTTGGATTAT GTAAGCATGT ACAGGAATAT GGGGAGTCCT 
TCTGCCAACA TCCCTGAGCA CTGGCACTAC ATCAGCTTTG GCCTGAGTGA TCTCTACGGT 
GACAACAGAG TCCACGAGTT TACAGGAACA GACGGACCAA GTGGATTTGG CTTTGAATTG 
ACATTTCGTC TGAAGAGAGA AACTGGGGAG TCTGCCCCAC CAACATGGCC AGCAGAGCTG 
ATGCAGGGCC TAGCCCGATA TGTCTTCCAG TCAGAGAATA CCTTCTGTAG TGGGGACCAC 
GTGTCTTGGC ACAGCCCTTT GGATAACAGT GAGTCAAGAA TTCAGCACAT GCTGCTGACG 
GAGGACCCAC AGATGCAGCC TGTGCAGACA CCCTTTGGGG TAGTTACTTT CCTCCAGATT 
GTTGGTGTCT GCACTGAGGA GTTACACTCA GCCCAGCAGT GGAATGGGCA GGGCATCCTG 
GAGCTGCTGC GGACAGTGCC CATTGCTGGC GGTCCCTGGC TGATAACTGA CATGCGGAGG 
GGAGAAACCA TATTCGAGAT CGATCCACAC CTGCAACAGG AGAGAGTTGA CAAAGGCATC 
GAGACAGACG GTTCTAACCT GAGCGGTGTC AGTGCCAAGT GTGCCTGGGA TGACCTCAGC 
CGGCCTCCGG AGGACGATGA GGATAGCCGG AGCATCTGCC TCGGCACGCA GCCTCGAAGG 
CTGTCTGGCA AAGACACAGA GCAGATCCGG GAGACCCTGA GGCGAGGACT GGAGATTAAC 
AGCAAACCTG TCCTTCCACC AATCAATTCT CAAAGGCAGA ATGGCCTCAC CTACGACCGA 
GCCCCGAGCC GTAAGGACAG CTTGGGCAGT GACATCTCCA CGGCTGTTAT CCCCCACGAG 
CTGATCCGCA CACGGCAGCT CGAAAGCGTG CATCTGAAAT TTAACCAGGA GTCGGGAGCC 
CTCATCCCCC TCTGCCTAAG GGGCAGACTC CTGCATGGCC GGCACTTCAC CTACAAGAGT 
ATCACAGGTG ACATGGCCAT CACGTTTGTG TCCACCGGAG TGGAAGGCGC CTTTGCCACT 
GAGGAACATC CTTACGCAGC TCACGGACCC TGGTTACAAA TTCTGTTGAC AGAAGAGTTT 
GTAGAGAAAA TGTTGGAGGA CTTAGAAGAT CTAACTTCTC CAGAGGAATT TAAACTTCCC 
AAAGAGTACA GCTGGCCTGA AAAGAAGCTC AAAGTGTCCA TCCTCCCCGA CGTGGTGTTC 
GACAGTCCGC TGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020070.1
CDS	171..1628
Translation	MAELRPSVAPGPAAPPASGPSAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDYVSMYRNMGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTYDRAPSRKDSLGSDISTAVIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH

Target ORF information:

RefSeq Version	NM_001024899.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024899.1

ORF Insert Sequence:

ATGGCGGAGC TGAGGCCTAG CGTCGCCCCC GGCCCCGCCG CGCCCCCGGC CTCTGGCCCT 
AGTGCCCCCC CGGCCTTTGC TTCACTCTTT CCCCCGGGAC TCCACGCCAT CTACGGAGAG 
TGCCGCCGCC TCTACCCTGA CCAGCCGAAC CCGCTCCAGG TTACCGCTAT CGTCAAGTAC 
TGGTTGGGTG GTCCAGACCC CTTGGATTAT GTAAGCATGT ACAGGAATAT GGGGAGTCCT 
TCTGCCAACA TCCCTGAGCA CTGGCACTAC ATCAGCTTTG GCCTGAGTGA TCTCTACGGT 
GACAACAGAG TCCACGAGTT TACAGGAACA GACGGACCAA GTGGATTTGG CTTTGAATTG 
ACATTTCGTC TGAAGAGAGA AACTGGGGAG TCTGCCCCAC CAACATGGCC AGCAGAGCTG 
ATGCAGGGCC TAGCCCGATA TGTCTTCCAG TCAGAGAATA CCTTCTGTAG TGGGGACCAC 
GTGTCTTGGC ACAGCCCTTT GGATAACAGT GAGTCAAGAA TTCAGCACAT GCTGCTGACG 
GAGGACCCAC AGATGCAGCC TGTGCAGACA CCCTTTGGGG TAGTTACTTT CCTCCAGATT 
GTTGGTGTCT GCACTGAGGA GTTACACTCA GCCCAGCAGT GGAATGGGCA GGGCATCCTG 
GAGCTGCTGC GGACAGTGCC CATTGCTGGC GGTCCCTGGC TGATAACTGA CATGCGGAGG 
GGAGAAACCA TATTCGAGAT CGATCCACAC CTGCAACAGG AGAGAGTTGA CAAAGGCATC 
GAGACAGACG GTTCTAACCT GAGCGGTGTC AGTGCCAAGT GTGCCTGGGA TGACCTCAGC 
CGGCCTCCGG AGGACGATGA GGATAGCCGG AGCATCTGCC TCGGCACGCA GCCTCGAAGG 
CTGTCTGGCA AAGACACAGA GCAGATCCGG GAGACCCTGA GGCGAGGACT GGAGATTAAC 
AGCAAACCTG TCCTTCCACC AATCAATTCT CAAAGGCAGA ATGGCCTCAC CTACGACCGA 
GCCCCGAGCC GTAAGGACAG CTTGGGCAGT GACATCTCCA CGGCTGTTAT CCCCCACGAG 
CTGATCCGCA CACGGCAGCT CGAAAGCGTG CATCTGAAAT TTAACCAGGA GTCGGGAGCC 
CTCATCCCCC TCTGCCTAAG GGGCAGACTC CTGCATGGCC GGCACTTCAC CTACAAGAGT 
ATCACAGGTG ACATGGCCAT CACGTTTGTG TCCACCGGAG TGGAAGGCGC CTTTGCCACT 
GAGGAACATC CTTACGCAGC TCACGGACCC TGGTTACAAA TTCTGTTGAC AGAAGAGTTT 
GTAGAGAAAA TGTTGGAGGA CTTAGAAGAT CTAACTTCTC CAGAGGAATT TAAACTTCCC 
AAAGAGTACA GCTGGCCTGA AAAGAAGCTC AAAGTGTCCA TCCTCCCCGA CGTGGTGTTC 
GACAGTCCGC TGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sufu ( XM_006231546.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231608.1 Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24202
Clone ID Related Accession (Same CDS sequence)	XM_006231546.3
Accession Version	XM_006231546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	suppressor of fused homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC TGAGGCCTAG CGTCGCCCCC GGCCCCGCCG CGCCCCCGGC CTCTGGCCCT 
AGTGCCCCCC CGGCCTTTGC TTCACTCTTT CCCCCGGGAC TCCACGCCAT CTACGGAGAG 
TGCCGCCGCC TCTACCCTGA CCAGCCGAAC CCGCTCCAGG TTACCGCTAT CGTCAAGTAC 
TGGTTGGGTG GTCCAGACCC CTTGGATTAT GTAAGCATGT ACAGGAATAT GGGGAGTCCT 
TCTGCCAACA TCCCTGAGCA CTGGCACTAC ATCAGCTTTG GCCTGAGTGA TCTCTACGGT 
GACAACAGAG TCCACGAGTT TACAGGAACA GACGGACCAA GTGGATTTGG CTTTGAATTG 
ACATTTCGTC TGAAGAGAGA AACTGGGGAG TCTGCCCCAC CAACATGGCC AGCAGAGCTG 
ATGCAGGGCC TAGCCCGATA TGTCTTCCAG TCAGAGAATA CCTTCTGTAG TGGGGACCAC 
GTGTCTTGGC ACAGCCCTTT GGATAACAGT GAGTCAAGAA TTCAGCACAT GCTGCTGACG 
GAGGACCCAC AGATGCAGCC TGTGCAGACA CCCTTTGGGG TAGTTACTTT CCTCCAGATT 
GTTGGTGTCT GCACTGAGGA GTTACACTCA GCCCAGCAGT GGAATGGGCA GGGCATCCTG 
GAGCTGCTGC GGACAGTGCC CATTGCTGGC GGTCCCTGGC TGATAACTGA CATGCGGAGG 
GGAGAAACCA TATTCGAGAT CGATCCACAC CTGCAAGAGA GAGTTGACAA AGGCATCGAG 
ACAGACGGTT CTAACCTGAG CGGTGTCAGT GCCAAGTGTG CCTGGGATGA CCTCAGCCGG 
CCTCCGGAGG ACGATGAGGA TAGCCGGAGC ATCTGCCTCG GCACGCAGCC TCGAAGGCTG 
TCTGGCAAAG ACACAGAGCA GATCCGGGAG ACCCTGAGGC GAGGACTGGA GATTAACAGC 
AAACCTGTCC TTCCACCAAT CAATTCTCAA AGGCAGAATG GCCTCACCTA CGACCGAGCC 
CCGAGCCGTA AGGACAGCTT GGGCAGTGAC ATCTCCACGG CTGTTATCCC CCACGAGCTG 
ATCCGCACAC GGCAGCTCGA AAGCGTGCAT CTGAAATTTA ACCAGGAGTC GGGAGCCCTC 
ATCCCCCTCT GCCTAAGGGG CAGACTCCTG CATGGCCGGC ACTTCACCTA CAAGAGTATC 
ACAGGTGACA TGGCCATCAC GTTTGTGTCC ACCGGAGTGG AAGGCGCCTT TGCCACTGAG 
GAACATCCTT ACGCAGCTCA CGGACCCTGG TTACAAATTC TGTTGACAGA AGAGTTTGTA 
GAGAAAATGT TGGAGGACTT AGAAGATCTA ACTTCTCCAG AGGAATTTAA ACTTCCCAAA 
GAGTACAGCT GGCCTGAAAA GAAGCTCAAA GTGTCCATCC TCCCCGACGT GGTGTTCGAC 
AGTCCGCTGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231608.1
CDS	223..1677
Translation	MAELRPSVAPGPAAPPASGPSAPPAFASLFPPGLHAIYGECRRLYPDQPNPLQVTAIVKYWLGGPDPLDYVSMYRNMGSPSANIPEHWHYISFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTYDRAPSRKDSLGSDISTAVIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH

Target ORF information:

RefSeq Version	XM_006231546.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231546.3

ORF Insert Sequence:

ATGGCGGAGC TGAGGCCTAG CGTCGCCCCC GGCCCCGCCG CGCCCCCGGC CTCTGGCCCT 
AGTGCCCCCC CGGCCTTTGC TTCACTCTTT CCCCCGGGAC TCCACGCCAT CTACGGAGAG 
TGCCGCCGCC TCTACCCTGA CCAGCCGAAC CCGCTCCAGG TTACCGCTAT CGTCAAGTAC 
TGGTTGGGTG GTCCAGACCC CTTGGATTAT GTAAGCATGT ACAGGAATAT GGGGAGTCCT 
TCTGCCAACA TCCCTGAGCA CTGGCACTAC ATCAGCTTTG GCCTGAGTGA TCTCTACGGT 
GACAACAGAG TCCACGAGTT TACAGGAACA GACGGACCAA GTGGATTTGG CTTTGAATTG 
ACATTTCGTC TGAAGAGAGA AACTGGGGAG TCTGCCCCAC CAACATGGCC AGCAGAGCTG 
ATGCAGGGCC TAGCCCGATA TGTCTTCCAG TCAGAGAATA CCTTCTGTAG TGGGGACCAC 
GTGTCTTGGC ACAGCCCTTT GGATAACAGT GAGTCAAGAA TTCAGCACAT GCTGCTGACG 
GAGGACCCAC AGATGCAGCC TGTGCAGACA CCCTTTGGGG TAGTTACTTT CCTCCAGATT 
GTTGGTGTCT GCACTGAGGA GTTACACTCA GCCCAGCAGT GGAATGGGCA GGGCATCCTG 
GAGCTGCTGC GGACAGTGCC CATTGCTGGC GGTCCCTGGC TGATAACTGA CATGCGGAGG 
GGAGAAACCA TATTCGAGAT CGATCCACAC CTGCAAGAGA GAGTTGACAA AGGCATCGAG 
ACAGACGGTT CTAACCTGAG CGGTGTCAGT GCCAAGTGTG CCTGGGATGA CCTCAGCCGG 
CCTCCGGAGG ACGATGAGGA TAGCCGGAGC ATCTGCCTCG GCACGCAGCC TCGAAGGCTG 
TCTGGCAAAG ACACAGAGCA GATCCGGGAG ACCCTGAGGC GAGGACTGGA GATTAACAGC 
AAACCTGTCC TTCCACCAAT CAATTCTCAA AGGCAGAATG GCCTCACCTA CGACCGAGCC 
CCGAGCCGTA AGGACAGCTT GGGCAGTGAC ATCTCCACGG CTGTTATCCC CCACGAGCTG 
ATCCGCACAC GGCAGCTCGA AAGCGTGCAT CTGAAATTTA ACCAGGAGTC GGGAGCCCTC 
ATCCCCCTCT GCCTAAGGGG CAGACTCCTG CATGGCCGGC ACTTCACCTA CAAGAGTATC 
ACAGGTGACA TGGCCATCAC GTTTGTGTCC ACCGGAGTGG AAGGCGCCTT TGCCACTGAG 
GAACATCCTT ACGCAGCTCA CGGACCCTGG TTACAAATTC TGTTGACAGA AGAGTTTGTA 
GAGAAAATGT TGGAGGACTT AGAAGATCTA ACTTCTCCAG AGGAATTTAA ACTTCCCAAA 
GAGTACAGCT GGCCTGAAAA GAAGCTCAAA GTGTCCATCC TCCCCGACGT GGTGTTCGAC 
AGTCCGCTGC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sufu ( XM_008760443.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758665.1 Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa48010
Clone ID Related Accession (Same CDS sequence)	XM_008760443.2
Accession Version	XM_008760443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	suppressor of fused homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTAT GTGACTGTAA TCCCAGCACT TGGGAAGCAG AGTCACAAGG ATCACAAATT 
TGTAGCCTAT ATTTAAATAA AAAATTTATA AATGTGTTTA TTTGCATGTA TGTGTGTTCT 
TGTAGTGACT CTGGGTGCCC AAGAGGCCAA AAGATGGAGT TACAGGCTGT TGTGAGCCTG 
ATGTGGGCAC TAGAAACTGA CCCTGATCGT CTGCAAGAGC AGCAAGTGCT CCTAACCGCT 
GAGCTTTCTC TCGGGATCCT TGTGTCCAGA ATACACAGAG TATTCACAGA GTACACACAA 
ATGCCATGGT TTACAGGAAC AGACGGACCA AGTGGATTTG GCTTTGAATT GACATTTCGT 
CTGAAGAGAG AAACTGGGGA GTCTGCCCCA CCAACATGGC CAGCAGAGCT GATGCAGGGC 
CTAGCCCGAT ATGTCTTCCA GTCAGAGAAT ACCTTCTGTA GTGGGGACCA CGTGTCTTGG 
CACAGCCCTT TGGATAACAG TGAGTCAAGA ATTCAGCACA TGCTGCTGAC GGAGGACCCA 
CAGATGCAGC CTGTGCAGAC ACCCTTTGGG GTAGTTACTT TCCTCCAGAT TGTTGGTGTC 
TGCACTGAGG AGTTACACTC AGCCCAGCAG TGGAATGGGC AGGGCATCCT GGAGCTGCTG 
CGGACAGTGC CCATTGCTGG CGGTCCCTGG CTGATAACTG ACATGCGGAG GGGAGAAACC 
ATATTCGAGA TCGATCCACA CCTGCAACAG GAGAGAGTTG ACAAAGGCAT CGAGACAGAC 
GGTTCTAACC TGAGCGGTGT CAGTGCCAAG TGTGCCTGGG ATGACCTCAG CCGGCCTCCG 
GAGGACGATG AGGATAGCCG GAGCATCTGC CTCGGCACGC AGCCTCGAAG GCTGTCTGGC 
AAAGACACAG AGCAGATCCG GGAGACCCTG AGGCGAGGAC TGGAGATTAA CAGCAAACCT 
GTCCTTCCAC CAATCAATTC TCAAAGGCAG AATGGCCTCA CCTACGACCG AGCCCCGAGC 
CGTAAGGACA GCTTGGGCAG TGACATCTCC ACGGCTGTTA TCCCCCACGA GCTGATCCGC 
ACACGGCAGC TCGAAAGCGT GCATCTGAAA TTTAACCAGG AGTCGGGAGC CCTCATCCCC 
CTCTGCCTAA GGGGCAGACT CCTGCATGGC CGGCACTTCA CCTACAAGAG TATCACAGGT 
GACATGGCCA TCACGTTTGT GTCCACCGGA GTGGAAGGCG CCTTTGCCAC TGAGGAACAT 
CCTTACGCAG CTCACGGACC CTGGTTACAA ATTCTGTTGA CAGAAGAGTT TGTAGAGAAA 
ATGTTGGAGG ACTTAGAAGA TCTAACTTCT CCAGAGGAAT TTAAACTTCC CAAAGAGTAC 
AGCTGGCCTG AAAAGAAGCT CAAAGTGTCC ATCCTCCCCG ACGTGGTGTT CGACAGTCCG 
CTGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758665.1
CDS	246..1694
Translation	MVVCDCNPSTWEAESQGSQICSLYLNKKFINVFICMYVCSCSDSGCPRGQKMELQAVVSLMWALETDPDRLQEQQVLLTAELSLGILVSRIHRVFTEYTQMPWFTGTDGPSGFGFELTFRLKRETGESAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICLGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINSQRQNGLTYDRAPSRKDSLGSDISTAVIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH

Target ORF information:

RefSeq Version	XM_008760443.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SUFU negative regulator of hedgehog signaling (Sufu), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760443.2

ORF Insert Sequence:

ATGGTAGTAT GTGACTGTAA TCCCAGCACT TGGGAAGCAG AGTCACAAGG ATCACAAATT 
TGTAGCCTAT ATTTAAATAA AAAATTTATA AATGTGTTTA TTTGCATGTA TGTGTGTTCT 
TGTAGTGACT CTGGGTGCCC AAGAGGCCAA AAGATGGAGT TACAGGCTGT TGTGAGCCTG 
ATGTGGGCAC TAGAAACTGA CCCTGATCGT CTGCAAGAGC AGCAAGTGCT CCTAACCGCT 
GAGCTTTCTC TCGGGATCCT TGTGTCCAGA ATACACAGAG TATTCACAGA GTACACACAA 
ATGCCATGGT TTACAGGAAC AGACGGACCA AGTGGATTTG GCTTTGAATT GACATTTCGT 
CTGAAGAGAG AAACTGGGGA GTCTGCCCCA CCAACATGGC CAGCAGAGCT GATGCAGGGC 
CTAGCCCGAT ATGTCTTCCA GTCAGAGAAT ACCTTCTGTA GTGGGGACCA CGTGTCTTGG 
CACAGCCCTT TGGATAACAG TGAGTCAAGA ATTCAGCACA TGCTGCTGAC GGAGGACCCA 
CAGATGCAGC CTGTGCAGAC ACCCTTTGGG GTAGTTACTT TCCTCCAGAT TGTTGGTGTC 
TGCACTGAGG AGTTACACTC AGCCCAGCAG TGGAATGGGC AGGGCATCCT GGAGCTGCTG 
CGGACAGTGC CCATTGCTGG CGGTCCCTGG CTGATAACTG ACATGCGGAG GGGAGAAACC 
ATATTCGAGA TCGATCCACA CCTGCAACAG GAGAGAGTTG ACAAAGGCAT CGAGACAGAC 
GGTTCTAACC TGAGCGGTGT CAGTGCCAAG TGTGCCTGGG ATGACCTCAG CCGGCCTCCG 
GAGGACGATG AGGATAGCCG GAGCATCTGC CTCGGCACGC AGCCTCGAAG GCTGTCTGGC 
AAAGACACAG AGCAGATCCG GGAGACCCTG AGGCGAGGAC TGGAGATTAA CAGCAAACCT 
GTCCTTCCAC CAATCAATTC TCAAAGGCAG AATGGCCTCA CCTACGACCG AGCCCCGAGC 
CGTAAGGACA GCTTGGGCAG TGACATCTCC ACGGCTGTTA TCCCCCACGA GCTGATCCGC 
ACACGGCAGC TCGAAAGCGT GCATCTGAAA TTTAACCAGG AGTCGGGAGC CCTCATCCCC 
CTCTGCCTAA GGGGCAGACT CCTGCATGGC CGGCACTTCA CCTACAAGAG TATCACAGGT 
GACATGGCCA TCACGTTTGT GTCCACCGGA GTGGAAGGCG CCTTTGCCAC TGAGGAACAT 
CCTTACGCAG CTCACGGACC CTGGTTACAA ATTCTGTTGA CAGAAGAGTT TGTAGAGAAA 
ATGTTGGAGG ACTTAGAAGA TCTAACTTCT CCAGAGGAAT TTAAACTTCC CAAAGAGTAC 
AGCTGGCCTG AAAAGAAGCT CAAAGTGTCC ATCCTCCCCG ACGTGGTGTT CGACAGTCCG 
CTGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-874 targeting SUFU involves in osteoblast proliferation and differentiation in osteoporosis rats through the Hedgehog signaling pathway.
Biochemical and biophysical research communications506(1)194-203(2018 11)
Lin JC,Liu ZG,Yu B,Zhang XR

Hypomorphic Recessive Variants in SUFU Impair the Sonic Hedgehog Pathway and Cause Joubert Syndrome with Cranio-facial and Skeletal Defects.
American journal of human genetics101(4)552-563(2017 Oct)
De Mori R,Romani M,D'Arrigo S,Zaki MS,Lorefice E,Tardivo S,Biagini T,Stanley V,Musaev D,Fluss J,Micalizzi A,Nuovo S,Illi B,Chiapparini L,Di Marcotullio L,Issa MY,Anello D,Casella A,Ginevrino M,Leggins AS,Roosing S,Alfonsi R,Rosati J,Schot R,Mancini GMS,Bertini E,Dobyns WB,Mazza T,Gleeson JG,Valente EM

SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development.
The EMBO journal35(13)1400-16(2016 07)
Raducu M,Fung E,Serres S,Infante P,Barberis A,Fischer R,Bristow C,Thézénas ML,Finta C,Christianson JC,Buffa FM,Kessler BM,Sibson NR,Di Marcotullio L,Toftgård R,D'Angiolella V

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Modulation of the transactivation function and stability of Krüppel-like zinc finger protein Gli-similar 3 (Glis3) by Suppressor of Fused.
The Journal of biological chemistry286(25)22077-89(2011 Jun)
ZeRuth GT,Yang XP,Jetten AM

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Sufu cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM