Phkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phkb
Entrez Gene ID	361377
Full Name	phosphorylase kinase regulatory subunit beta
General protein information	Preferred Names phosphorylase kinase regulatory subunit beta Names phosphorylase b kinase regulatory subunit beta phosphorylase kinase beta subunit phosphorylase kinase, beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p11
Summary	expression in regenerating muscle may be correlated with that of other phosphorylase kinase subunits [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772410.1	XP_008770632.1	phosphorylase b kinase regulatory subunit beta isoform X3
NM_001014152.1	NP_001014174.1	phosphorylase b kinase regulatory subunit beta
XM_006255269.3	XP_006255331.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_006255270.3	XP_006255332.1	phosphorylase b kinase regulatory subunit beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHKB	NP_000284.1
Caenorhabditis elegans (roundworm)	Y67D8A.2	NP_741355.3
Xenopus tropicalis (tropical clawed frog)	phkb	XP_004914116.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009278	XP_320073.4
Macaca mulatta (Rhesus monkey)	PHKB	XP_001113858.1
Danio rerio (zebrafish)	phkb	XP_698197.5
Canis lupus familiaris (dog)	PHKB	XP_005617652.1
Mus musculus (house mouse)	Phkb	NP_955517.1
Pan troglodytes (chimpanzee)	PHKB	XP_001162360.1
Bos taurus (cattle)	PHKB	XP_002694819.1
Rattus norvegicus (Norway rat)	Phkb	NP_001014174.1
Gallus gallus (chicken)	PHKB	NP_001007832.1
Drosophila melanogaster (fruit fly)	CG8475	NP_001260230.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Brushia RJ, Walsh DA
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)

Coordinated expression of phosphorylase kinase subunits in regenerating skeletal muscle.
Cawley KC, Akita CG, Wineinger MA, Carlsen RC, Gorin FA, Walsh DA
The Journal of biological chemistry267(24)17287-95(1992 Aug)

The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.
Kilimann MW, Zander NF, Kuhn CC, Crabb JW, Meyer HE, Heilmeyer LM Jr
Proceedings of the National Academy of Sciences of the United States of America85(24)9381-5(1988 Dec)

Rat skeletal muscle phosphorylase kinase: turnover and control of isozyme levels in culture.
Salsgiver WJ, Lawrence JC Jr
The American journal of physiology250(3 Pt 1)C365-73(1986 Mar)

Purification of rat liver phosphorylase kinase.
Chrisman TD, Jordan JE, Exton JH
The Journal of biological chemistry257(18)10798-804(1982 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05153	NM_001014152.1 Latest version!	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa38714	XM_006255269.3 Latest version!	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa38715	XM_006255270.3 Latest version!	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa47900	XM_008772410.1 Latest version!	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phkb ( NM_001014152.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014174.1 Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), mRNA.

Price & Availability↑

CloneID	ORa05153
Clone ID Related Accession (Same CDS sequence)	NM_001014152.1
Accession Version	NM_001014152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527350400000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085909.1. On Apr 20, 2005 this sequence version replaced XM_341657.2. ##Evidence-Data-START## Transcript exon combination :: BC085909.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGTCG 
GTTGTACGCC GTGCAGCAAG TCTTTTAAAT AAAGTAGTGG ACAGCCTGGC CCCATCCATT 
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGTAAGT GGAGAGGCCC GTGTCCCACA GAACGCTTTC 
ATTGTTTCCA CTGTTGAAAT CCTCTCTAAT CTTCCCACTT TCCAGACAGA TGGACAGAGC 
ACAAAAGAGG ATGCTCTACA AGCAGTCAGG CTTACTGAGG AATTAATATT GCTTTTTAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014174.1
CDS	18..3320
Translation	MAGEEGLMSEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGLFPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQSSTADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWSVVRRAASLLNKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQLLLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQVSGEARVPQNAFIVSTVEILSNLPTFQTDGQSTKEDALQAVRLTEELILLFS

Target ORF information:

RefSeq Version	NM_001014152.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014152.1

ORF Insert Sequence:

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGTCG 
GTTGTACGCC GTGCAGCAAG TCTTTTAAAT AAAGTAGTGG ACAGCCTGGC CCCATCCATT 
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGTAAGT GGAGAGGCCC GTGTCCCACA GAACGCTTTC 
ATTGTTTCCA CTGTTGAAAT CCTCTCTAAT CTTCCCACTT TCCAGACAGA TGGACAGAGC 
ACAAAAGAGG ATGCTCTACA AGCAGTCAGG CTTACTGAGG AATTAATATT GCTTTTTAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phkb ( XM_006255269.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255331.1 Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38714
Clone ID Related Accession (Same CDS sequence)	XM_006255269.3
Accession Version	XM_006255269.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255269.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGTCG 
GTTGTACGCC GTGCAGCAAG TCTTTTAAAT AAAGTAGTGG ACAGCCTGGC CCCATCCATT 
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGACGGT ATGACTTCCT TCTACAACAC GCCCCCACTG 
GGACAAAGAG GAACCTGCAG CTACTTGACA AAGGTGGTGA TGAACTCCCT GCTGGAGGGA 
GAAGTCAAGC CCACCAATGA GGACTCGTGC CTGGTTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255331.1
CDS	28..3309
Translation	MAGEEGLMSEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGLFPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQSSTADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWSVVRRAASLLNKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQLLLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDGMTSFYNTPPLGQRGTCSYLTKVVMNSLLEGEVKPTNEDSCLVS

Target ORF information:

RefSeq Version	XM_006255269.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255269.3

ORF Insert Sequence:

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGTCG 
GTTGTACGCC GTGCAGCAAG TCTTTTAAAT AAAGTAGTGG ACAGCCTGGC CCCATCCATT 
ACTAATGTTT TAGTGCAGGG CAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGACGGT ATGACTTCCT TCTACAACAC GCCCCCACTG 
GGACAAAGAG GAACCTGCAG CTACTTGACA AAGGTGGTGA TGAACTCCCT GCTGGAGGGA 
GAAGTCAAGC CCACCAATGA GGACTCGTGC CTGGTTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phkb ( XM_006255270.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255332.1 Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38715
Clone ID Related Accession (Same CDS sequence)	XM_006255270.3
Accession Version	XM_006255270.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGCTG 
GCTGTGCGGT ACGGGGCTGC ATTTACCCAG AAATTTTCCT CCTCTATAGC CCCGCACATT 
ACCACCTTTC TGGTACATGG GAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGACGGT ATGACTTCCT TCTACAACAC GCCCCCACTG 
GGACAAAGAG GAACCTGCAG CTACTTGACA AAGGTGGTGA TGAACTCCCT GCTGGAGGGA 
GAAGTCAAGC CCACCAATGA GGACTCGTGC CTGGTTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255332.1
CDS	28..3309
Translation	MAGEEGLMSEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGLFPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQSSTADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQLLLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDGMTSFYNTPPLGQRGTCSYLTKVVMNSLLEGEVKPTNEDSCLVS

Target ORF information:

RefSeq Version	XM_006255270.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255270.3

ORF Insert Sequence:

ATGGCGGGGG AAGAGGGACT CATGTCTGAA GTAAGCTGGA AGCTCTTGGA ACGACGAGCC 
CGGGCCAAGC GCTCAGGCTC AGTTTATGAA CCTCTTAAAA GCATTAATCT TCCAAGACCT 
GATAATGAAA CTCTATGGGA TAAGTTGGAT CATTATTACA GAATTGTCAA GTCAACGATG 
CTGACATATC AAAGCCCAAC TACAGGTCTC TTTCCTACTA AAACTTGTGG TGGAGATCAG 
AAGTCCAAAG TGCATGAGAG CCTGTACTGT GCTGCGGGTG CTTGGGCCTT GGCTCTGGCG 
TACAGGCGCA TTGATGATGA CAAGGGAAGG ACCCATGAGC TGGAGCACTC GGCCATAAAG 
TGTATGAGAG GAGTTCTCTA CTGCTACATG CGGCAGGCTG ATAAGGTCCA GCAGTTCAAG 
CAGGATCCCC GGCCCACGAC GTGTCTACAC TCTGTTTTCA ATGTGCACAC TGGGGATGAA 
GTGCTCTCCT ATGAAGAATA TGGCCATCTT CAGATAAATG CAGTCTCACT CTTTCTCCTT 
TACCTGGTGG AAATGATTTC CTCAGGTCTC CAGATAATCT ATAACACGGA TGAGGTCTCT 
TTTATTCAAA ACCTTGTGTT TTGTGTAGAG AGAGTTTACC GTGTTCCCGA CTTCGGTGTC 
TGGGAAAGAG GAAGCAAATA TAACAACGGC AGCACAGAAC TGCACTCGAG CTCTGTTGGT 
TTGGCAAAAG CAGCCTTAGA AGCGATTAAT GGATTTAACC TCTTTGGAAA CCAGGGCTGT 
TCTTGGTCAG TTATATTCGT GGATCTCGAT GCTCACAACC GCAACAGGCA GACGTTGTGT 
TCACTGTTAC CCAGAGAATC ACGCTCTCAT AACACAGATG CTGCCCTGCT CCCGTGCATC 
ACCTACCCCG CCTTTGCCCT GGACGATGAA GCTCTGTTCA GCCAGACACT CGATAAAGTG 
ATAAGAAAAT TAAAAGGGAA ATATGGATTT AAGCGTTTCT TGCGAGATGG GTACAGAACG 
CCCCTGGAAG ACCCCAACAG ACGCTACTAC AAACCAGCGG AGATTAAGCT GTTTGATGGC 
ATCGAATGTG AATTTCCCAT ATTCTTCCTT TACCTGATGA TCGATGGTGT TTTTAGAGGC 
AATCTTGAAC AAGTCAAGGA ATATCAGGAC CTGTTGACAC CTTTGCTTCA CCAGACCACA 
GAAGGATATC CTGTTGTACC AAAGTACTAT TATGTGCCAG CTGACTTTGT AGAATATGAA 
AAAAGAAACC CTGGTAGTCA GAAGCGGTTT CCTAGTAACT GTGGCCGTGA TGGGAAATTG 
TTTCTTTGGG GACAAGCTCT TTATATCATT GCAAAACTCC TAGCTGATGA GTTAATCAGT 
CCTAAAGACA TTGACCCCGT CCATCGCTTT GTCCCACTTC AGAATCAACG TAACGTGAGC 
ATGAGATTCT CTAATCAGGG CCCACTGGAA AATGACTTGG TGGTTCATGT GGCACTTGTA 
GCAGAAAGCC AACGCCTTCA GGTCTTCCTC AATACTTACG GGATTCAGAC TCAAACGCCC 
CAGCAGGTGG AGCCTATTCA GATCTGGCCT CAGCAGGAGC TCGTGAAAGC TTATTTCCAC 
CTGGGTATCA ATGAAAAGTT GGGTCTGTCT GGAAGGCCAG ATAGGCCCAT TGGCTGCCTT 
GGTACTTCAA AGATTTACCG CATCCTAGGA AGGACCGTGG TTTGTTACCC TATCATCTTC 
GACCTCAGTG ATTTCTACAT GTCTCAGGAC GTTCTCCTGC TCATAGATGA CATAAAGAAT 
GCACTACAGT TCATTAAGCA GTATTGGAAA ATGCATGGAC GCCCACTTTT TCTTGTTCTC 
ATCCGGGAAG ACAACATAAG AGGTAGCAGA TTCAACCCCA TATTAGACAT GCTGGCAGCC 
TTTAAAAAGG GGATAATTGG AGGAGTCAAA GTTCATGTTG ACCGTTTACA GACATTGATA 
TCTGGAGCTG TAGTAGAACA GCTTGACTTT CTGCGGATTA GCGACACAGA AAAGCTTCCA 
GAGTTTAAGA GCTTTGAAGA ACTAGAATTT CCCAAGCATT CAAAAGTCAA ACGACAGAGC 
AGTACTGCAG ATGCTCCTGA ACTGCGACAG GAGCCAGACA TCACCATCAC AGAGTGGAAA 
AACAAATCCA CCCACGAAAT CCTTCAGAAG CTTAATGACT GCGGTTGTCT GGCTGGCCAA 
ACCATCCTGC TGGGTATACT GCTCAAAAGA GAGGGCCCTA ACTTCATCAC GATGGAAGGA 
ACTGTGTCTG ATCACATTGA GAGGGTGTAC AGAAGAGCTG GCAGCAAAAA GCTTTGGCTG 
GCTGTGCGGT ACGGGGCTGC ATTTACCCAG AAATTTTCCT CCTCTATAGC CCCGCACATT 
ACCACCTTTC TGGTACATGG GAAACAGGTA ACCCTAGGTG CCTTTGGACA CGAAGAGGAG 
GTCATCTCTA ATCCACTATC TCCAAGAGTG ATTAAGAACA TCATCTATTA TAAGTGTAAC 
ACCCATGATG AGAGGGAGGC TGTCATTCAG CAAGAGCTGG TCATCCATAT TGGCTGGATC 
ATCTCCAACA ACCCCGAGCT GTTCAGTGGC ATGCTGAAAA TCAGAATTGG GTGGATCATC 
CATGCCATGG AGTACGAACT GCAGATCCGT GGCGGGGATA AGCCAGCTGT GGACTTGTAC 
CAGCTCTCGC CCAGTGAGGT TAAGCAGCTG CTGCTCGACA TCCTGCAGCC TCAGCAGAGT 
GGGAGATGTT GGCTGAATAG ACGTCAGATT GATGGGTCAC TGAATAGAAC ACCCCCAGGA 
TTCTACGACC GCGTGTGGCA GATCCTGGAG CGCACACCTA ATGGTATCGT TGTAGCTGGC 
AAGCATTTGC CACAGCAACC AACCTTGTCA GATATGACCA TGTATGAGAT GAATTTTTCT 
CTCCTTGTTG AAGATATGTT GGGAAACATT GACCAGCCAA AGTATAGACA GATCATTGTA 
GAGTTACTAA TGGTTGTATC CATTGTGCTG GAAAGAAATC CTGAGCTAGA ATTTCAAGAC 
AAAGTGGATC TGGACAAACT GGTCAAAGAA GCGTTTCATG AGTTTCAAAA AGATGAGAGC 
CGGCTAAAAG AAATCGAAAA GCAAGACGGT ATGACTTCCT TCTACAACAC GCCCCCACTG 
GGACAAAGAG GAACCTGCAG CTACTTGACA AAGGTGGTGA TGAACTCCCT GCTGGAGGGA 
GAAGTCAAGC CCACCAATGA GGACTCGTGC CTGGTTAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phkb ( XM_008772410.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770632.1 Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa47900
Clone ID Related Accession (Same CDS sequence)	XM_008772410.1
Accession Version	XM_008772410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACT CACCTGATGC ATCCTTCTCT TCACCTGCTT TGTTAAGGTC TGGCTCAGTT 
TATGAACCTC TTAAAAGCAT TAATCTTCCA AGACCTGATA ATGAAACTCT ATGGGATAAG 
TTGGATCATT ATTACAGAAT TGTCAAGTCA ACGATGCTGA CATATCAAAG CCCAACTACA 
GGTCTCTTTC CTACTAAAAC TTGTGGTGGA GATCAGAAGT CCAAAGTGCA TGAGAGCCTG 
TACTGTGCTG CGGGTGCTTG GGCCTTGGCT CTGGCGTACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCC ATAAAGTGTA TGAGAGGAGT TCTCTACTGC 
TACATGCGGC AGGCTGATAA GGTCCAGCAG TTCAAGCAGG ATCCCCGGCC CACGACGTGT 
CTACACTCTG TTTTCAATGT GCACACTGGG GATGAAGTGC TCTCCTATGA AGAATATGGC 
CATCTTCAGA TAAATGCAGT CTCACTCTTT CTCCTTTACC TGGTGGAAAT GATTTCCTCA 
GGTCTCCAGA TAATCTATAA CACGGATGAG GTCTCTTTTA TTCAAAACCT TGTGTTTTGT 
GTAGAGAGAG TTTACCGTGT TCCCGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAC 
AACGGCAGCA CAGAACTGCA CTCGAGCTCT GTTGGTTTGG CAAAAGCAGC CTTAGAAGCG 
ATTAATGGAT TTAACCTCTT TGGAAACCAG GGCTGTTCTT GGTCAGTTAT ATTCGTGGAT 
CTCGATGCTC ACAACCGCAA CAGGCAGACG TTGTGTTCAC TGTTACCCAG AGAATCACGC 
TCTCATAACA CAGATGCTGC CCTGCTCCCG TGCATCACCT ACCCCGCCTT TGCCCTGGAC 
GATGAAGCTC TGTTCAGCCA GACACTCGAT AAAGTGATAA GAAAATTAAA AGGGAAATAT 
GGATTTAAGC GTTTCTTGCG AGATGGGTAC AGAACGCCCC TGGAAGACCC CAACAGACGC 
TACTACAAAC CAGCGGAGAT TAAGCTGTTT GATGGCATCG AATGTGAATT TCCCATATTC 
TTCCTTTACC TGATGATCGA TGGTGTTTTT AGAGGCAATC TTGAACAAGT CAAGGAATAT 
CAGGACCTGT TGACACCTTT GCTTCACCAG ACCACAGAAG GATATCCTGT TGTACCAAAG 
TACTATTATG TGCCAGCTGA CTTTGTAGAA TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGGTTTCCTA GTAACTGTGG CCGTGATGGG AAATTGTTTC TTTGGGGACA AGCTCTTTAT 
ATCATTGCAA AACTCCTAGC TGATGAGTTA ATCAGTCCTA AAGACATTGA CCCCGTCCAT 
CGCTTTGTCC CACTTCAGAA TCAACGTAAC GTGAGCATGA GATTCTCTAA TCAGGGCCCA 
CTGGAAAATG ACTTGGTGGT TCATGTGGCA CTTGTAGCAG AAAGCCAACG CCTTCAGGTC 
TTCCTCAATA CTTACGGGAT TCAGACTCAA ACGCCCCAGC AGGTGGAGCC TATTCAGATC 
TGGCCTCAGC AGGAGCTCGT GAAAGCTTAT TTCCACCTGG GTATCAATGA AAAGTTGGGT 
CTGTCTGGAA GGCCAGATAG GCCCATTGGC TGCCTTGGTA CTTCAAAGAT TTACCGCATC 
CTAGGAAGGA CCGTGGTTTG TTACCCTATC ATCTTCGACC TCAGTGATTT CTACATGTCT 
CAGGACGTTC TCCTGCTCAT AGATGACATA AAGAATGCAC TACAGTTCAT TAAGCAGTAT 
TGGAAAATGC ATGGACGCCC ACTTTTTCTT GTTCTCATCC GGGAAGACAA CATAAGAGGT 
AGCAGATTCA ACCCCATATT AGACATGCTG GCAGCCTTTA AAAAGGGGAT AATTGGAGGA 
GTCAAAGTTC ATGTTGACCG TTTACAGACA TTGATATCTG GAGCTGTAGT AGAACAGCTT 
GACTTTCTGC GGATTAGCGA CACAGAAAAG CTTCCAGAGT TTAAGAGCTT TGAAGAACTA 
GAATTTCCCA AGCATTCAAA AGTCAAACGA CAGAGCAGTA CTGCAGATGC TCCTGAACTG 
CGACAGGAGC CAGACATCAC CATCACAGAG TGGAAAAACA AATCCACCCA CGAAATCCTT 
CAGAAGCTTA ATGACTGCGG TTGTCTGGCT GGCCAAACCA TCCTGCTGGG TATACTGCTC 
AAAAGAGAGG GCCCTAACTT CATCACGATG GAAGGAACTG TGTCTGATCA CATTGAGAGG 
GTGTACAGAA GAGCTGGCAG CAAAAAGCTT TGGCTGGCTG TGCGGTACGG GGCTGCATTT 
ACCCAGAAAT TTTCCTCCTC TATAGCCCCG CACATTACCA CCTTTCTGGT ACATGGGAAA 
CAGGTAACCC TAGGTGCCTT TGGACACGAA GAGGAGGTCA TCTCTAATCC ACTATCTCCA 
AGAGTGATTA AGAACATCAT CTATTATAAG TGTAACACCC ATGATGAGAG GGAGGCTGTC 
ATTCAGCAAG AGCTGGTCAT CCATATTGGC TGGATCATCT CCAACAACCC CGAGCTGTTC 
AGTGGCATGC TGAAAATCAG AATTGGGTGG ATCATCCATG CCATGGAGTA CGAACTGCAG 
ATCCGTGGCG GGGATAAGCC AGCTGTGGAC TTGTACCAGC TCTCGCCCAG TGAGGTTAAG 
CAGCTGCTGC TCGACATCCT GCAGCCTCAG CAGAGTGGGA GATGTTGGCT GAATAGACGT 
CAGATTGATG GGTCACTGAA TAGAACACCC CCAGGATTCT ACGACCGCGT GTGGCAGATC 
CTGGAGCGCA CACCTAATGG TATCGTTGTA GCTGGCAAGC ATTTGCCACA GCAACCAACC 
TTGTCAGATA TGACCATGTA TGAGATGAAT TTTTCTCTCC TTGTTGAAGA TATGTTGGGA 
AACATTGACC AGCCAAAGTA TAGACAGATC ATTGTAGAGT TACTAATGGT TGTATCCATT 
GTGCTGGAAA GAAATCCTGA GCTAGAATTT CAAGACAAAG TGGATCTGGA CAAACTGGTC 
AAAGAAGCGT TTCATGAGTT TCAAAAAGAT GAGAGCCGGC TAAAAGAAAT CGAAAAGCAA 
GACGGTATGA CTTCCTTCTA CAACACGCCC CCACTGGGAC AAAGAGGAAC CTGCAGCTAC 
TTGACAAAGG TGGTGATGAA CTCCCTGCTG GAGGGAGAAG TCAAGCCCAC CAATGAGGAC 
TCGTGCCTGG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770632.1
CDS	791..4048
Translation	MANSPDASFSSPALLRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGLFPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYYKPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQSSTADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQLLLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQDGMTSFYNTPPLGQRGTCSYLTKVVMNSLLEGEVKPTNEDSCLVS

Target ORF information:

RefSeq Version	XM_008772410.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase regulatory subunit beta (Phkb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772410.1

ORF Insert Sequence:

ATGGCCAACT CACCTGATGC ATCCTTCTCT TCACCTGCTT TGTTAAGGTC TGGCTCAGTT 
TATGAACCTC TTAAAAGCAT TAATCTTCCA AGACCTGATA ATGAAACTCT ATGGGATAAG 
TTGGATCATT ATTACAGAAT TGTCAAGTCA ACGATGCTGA CATATCAAAG CCCAACTACA 
GGTCTCTTTC CTACTAAAAC TTGTGGTGGA GATCAGAAGT CCAAAGTGCA TGAGAGCCTG 
TACTGTGCTG CGGGTGCTTG GGCCTTGGCT CTGGCGTACA GGCGCATTGA TGATGACAAG 
GGAAGGACCC ATGAGCTGGA GCACTCGGCC ATAAAGTGTA TGAGAGGAGT TCTCTACTGC 
TACATGCGGC AGGCTGATAA GGTCCAGCAG TTCAAGCAGG ATCCCCGGCC CACGACGTGT 
CTACACTCTG TTTTCAATGT GCACACTGGG GATGAAGTGC TCTCCTATGA AGAATATGGC 
CATCTTCAGA TAAATGCAGT CTCACTCTTT CTCCTTTACC TGGTGGAAAT GATTTCCTCA 
GGTCTCCAGA TAATCTATAA CACGGATGAG GTCTCTTTTA TTCAAAACCT TGTGTTTTGT 
GTAGAGAGAG TTTACCGTGT TCCCGACTTC GGTGTCTGGG AAAGAGGAAG CAAATATAAC 
AACGGCAGCA CAGAACTGCA CTCGAGCTCT GTTGGTTTGG CAAAAGCAGC CTTAGAAGCG 
ATTAATGGAT TTAACCTCTT TGGAAACCAG GGCTGTTCTT GGTCAGTTAT ATTCGTGGAT 
CTCGATGCTC ACAACCGCAA CAGGCAGACG TTGTGTTCAC TGTTACCCAG AGAATCACGC 
TCTCATAACA CAGATGCTGC CCTGCTCCCG TGCATCACCT ACCCCGCCTT TGCCCTGGAC 
GATGAAGCTC TGTTCAGCCA GACACTCGAT AAAGTGATAA GAAAATTAAA AGGGAAATAT 
GGATTTAAGC GTTTCTTGCG AGATGGGTAC AGAACGCCCC TGGAAGACCC CAACAGACGC 
TACTACAAAC CAGCGGAGAT TAAGCTGTTT GATGGCATCG AATGTGAATT TCCCATATTC 
TTCCTTTACC TGATGATCGA TGGTGTTTTT AGAGGCAATC TTGAACAAGT CAAGGAATAT 
CAGGACCTGT TGACACCTTT GCTTCACCAG ACCACAGAAG GATATCCTGT TGTACCAAAG 
TACTATTATG TGCCAGCTGA CTTTGTAGAA TATGAAAAAA GAAACCCTGG TAGTCAGAAG 
CGGTTTCCTA GTAACTGTGG CCGTGATGGG AAATTGTTTC TTTGGGGACA AGCTCTTTAT 
ATCATTGCAA AACTCCTAGC TGATGAGTTA ATCAGTCCTA AAGACATTGA CCCCGTCCAT 
CGCTTTGTCC CACTTCAGAA TCAACGTAAC GTGAGCATGA GATTCTCTAA TCAGGGCCCA 
CTGGAAAATG ACTTGGTGGT TCATGTGGCA CTTGTAGCAG AAAGCCAACG CCTTCAGGTC 
TTCCTCAATA CTTACGGGAT TCAGACTCAA ACGCCCCAGC AGGTGGAGCC TATTCAGATC 
TGGCCTCAGC AGGAGCTCGT GAAAGCTTAT TTCCACCTGG GTATCAATGA AAAGTTGGGT 
CTGTCTGGAA GGCCAGATAG GCCCATTGGC TGCCTTGGTA CTTCAAAGAT TTACCGCATC 
CTAGGAAGGA CCGTGGTTTG TTACCCTATC ATCTTCGACC TCAGTGATTT CTACATGTCT 
CAGGACGTTC TCCTGCTCAT AGATGACATA AAGAATGCAC TACAGTTCAT TAAGCAGTAT 
TGGAAAATGC ATGGACGCCC ACTTTTTCTT GTTCTCATCC GGGAAGACAA CATAAGAGGT 
AGCAGATTCA ACCCCATATT AGACATGCTG GCAGCCTTTA AAAAGGGGAT AATTGGAGGA 
GTCAAAGTTC ATGTTGACCG TTTACAGACA TTGATATCTG GAGCTGTAGT AGAACAGCTT 
GACTTTCTGC GGATTAGCGA CACAGAAAAG CTTCCAGAGT TTAAGAGCTT TGAAGAACTA 
GAATTTCCCA AGCATTCAAA AGTCAAACGA CAGAGCAGTA CTGCAGATGC TCCTGAACTG 
CGACAGGAGC CAGACATCAC CATCACAGAG TGGAAAAACA AATCCACCCA CGAAATCCTT 
CAGAAGCTTA ATGACTGCGG TTGTCTGGCT GGCCAAACCA TCCTGCTGGG TATACTGCTC 
AAAAGAGAGG GCCCTAACTT CATCACGATG GAAGGAACTG TGTCTGATCA CATTGAGAGG 
GTGTACAGAA GAGCTGGCAG CAAAAAGCTT TGGCTGGCTG TGCGGTACGG GGCTGCATTT 
ACCCAGAAAT TTTCCTCCTC TATAGCCCCG CACATTACCA CCTTTCTGGT ACATGGGAAA 
CAGGTAACCC TAGGTGCCTT TGGACACGAA GAGGAGGTCA TCTCTAATCC ACTATCTCCA 
AGAGTGATTA AGAACATCAT CTATTATAAG TGTAACACCC ATGATGAGAG GGAGGCTGTC 
ATTCAGCAAG AGCTGGTCAT CCATATTGGC TGGATCATCT CCAACAACCC CGAGCTGTTC 
AGTGGCATGC TGAAAATCAG AATTGGGTGG ATCATCCATG CCATGGAGTA CGAACTGCAG 
ATCCGTGGCG GGGATAAGCC AGCTGTGGAC TTGTACCAGC TCTCGCCCAG TGAGGTTAAG 
CAGCTGCTGC TCGACATCCT GCAGCCTCAG CAGAGTGGGA GATGTTGGCT GAATAGACGT 
CAGATTGATG GGTCACTGAA TAGAACACCC CCAGGATTCT ACGACCGCGT GTGGCAGATC 
CTGGAGCGCA CACCTAATGG TATCGTTGTA GCTGGCAAGC ATTTGCCACA GCAACCAACC 
TTGTCAGATA TGACCATGTA TGAGATGAAT TTTTCTCTCC TTGTTGAAGA TATGTTGGGA 
AACATTGACC AGCCAAAGTA TAGACAGATC ATTGTAGAGT TACTAATGGT TGTATCCATT 
GTGCTGGAAA GAAATCCTGA GCTAGAATTT CAAGACAAAG TGGATCTGGA CAAACTGGTC 
AAAGAAGCGT TTCATGAGTT TCAAAAAGAT GAGAGCCGGC TAAAAGAAAT CGAAAAGCAA 
GACGGTATGA CTTCCTTCTA CAACACGCCC CCACTGGGAC AAAGAGGAAC CTGCAGCTAC 
TTGACAAAGG TGGTGATGAA CTCCCTGCTG GAGGGAGAAG TCAAGCCCAC CAATGAGGAC 
TCGTGCCTGG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)
Brushia RJ,Walsh DA

Coordinated expression of phosphorylase kinase subunits in regenerating skeletal muscle.
The Journal of biological chemistry267(24)17287-95(1992 Aug)
Cawley KC,Akita CG,Wineinger MA,Carlsen RC,Gorin FA,Walsh DA

The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.
Proceedings of the National Academy of Sciences of the United States of America85(24)9381-5(1988 Dec)
Kilimann MW,Zander NF,Kuhn CC,Crabb JW,Meyer HE,Heilmeyer LM Jr

Rat skeletal muscle phosphorylase kinase: turnover and control of isozyme levels in culture.
The American journal of physiology250(3 Pt 1)C365-73(1986 Mar)
Salsgiver WJ,Lawrence JC Jr

Purification of rat liver phosphorylase kinase.
The Journal of biological chemistry257(18)10798-804(1982 Sep)
Chrisman TD,Jordan JE,Exton JH

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Phkb cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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