PHKB cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHKB
Entrez Gene ID	478139
Full Name	phosphorylase kinase regulatory subunit beta
General protein information	Preferred Names phosphorylase kinase regulatory subunit beta Names phosphorylase b kinase regulatory subunit beta
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014109653.1	XP_013965128.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_858623.3	XP_863716.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_014109653.2	XP_013965128.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_022411649.1	XP_022267357.1	phosphorylase b kinase regulatory subunit beta isoform X3
XM_022411653.1	XP_022267361.1	phosphorylase b kinase regulatory subunit beta isoform X4
XM_858623.4	XP_863716.1	phosphorylase b kinase regulatory subunit beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHKB	NP_000284.1
Caenorhabditis elegans (roundworm)	Y67D8A.2	NP_741355.3
Xenopus tropicalis (tropical clawed frog)	phkb	XP_004914116.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009278	XP_320073.4
Macaca mulatta (Rhesus monkey)	PHKB	XP_001113858.1
Danio rerio (zebrafish)	phkb	XP_698197.5
Canis lupus familiaris (dog)	PHKB	XP_005617652.1
Mus musculus (house mouse)	Phkb	NP_955517.1
Pan troglodytes (chimpanzee)	PHKB	XP_001162360.1
Bos taurus (cattle)	PHKB	XP_002694819.1
Rattus norvegicus (Norway rat)	Phkb	NP_001014174.1
Gallus gallus (chicken)	PHKB	NP_001007832.1
Drosophila melanogaster (fruit fly)	CG8475	NP_001260230.1

Gene Ontology

The following PHKB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe286825	XM_022411653.1 Latest version!	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe03349	XM_858623.4 Latest version!	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe286824	XM_022411649.1 Latest version!	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe35416	XM_014109653.2 Latest version!	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe35416	XM_014109653.1	Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCe03349	XM_858623.3	Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHKB ( XM_022411653.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022267361.1 Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe286825
Clone ID Related Accession (Same CDS sequence)	XM_022411653.1
Accession Version	XM_022411653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGTCACAG AGAGAGAGAG 
AGAGGCAGAG ACACAGGCAG AGGGAGAAGC AGGCTCCATG CACCGGGAGC CCGACGTGGG 
ATTCGATCCC AGGTCTCCAG GATCGCGCCC TGGGCCAAAG GCAGGCGCCA AACCGCTGCG 
CCACCCAGGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022267361.1
CDS	187..3084
Translation	MASSPDAVISSPPAFLRSGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGHRERERGRDTGRGRSRLHAPGARRGIRSQVSRIAPWAKGRRQTAAPPRDP

Target ORF information:

RefSeq Version	XM_022411653.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411653.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGTCACAG AGAGAGAGAG 
AGAGGCAGAG ACACAGGCAG AGGGAGAAGC AGGCTCCATG CACCGGGAGC CCGACGTGGG 
ATTCGATCCC AGGTCTCCAG GATCGCGCCC TGGGCCAAAG GCAGGCGCCA AACCGCTGCG 
CCACCCAGGG ATCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_858623.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863716.1 Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03349
Clone ID Related Accession (Same CDS sequence)	XM_858623.3 , XM_858623.4
Accession Version	XM_858623.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_858623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863716.1
CDS	144..3404
Translation	MASSPDAVISSPPAFLRSGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGCWLNRRQIDGSLNRTPAGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKDAFHEFQKDESRLKEVEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_858623.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858623.3

ORF Insert Sequence:

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_858623.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_863716.1 Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03349
Clone ID Related Accession (Same CDS sequence)	XM_858623.3 , XM_858623.4
Accession Version	XM_858623.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_858623.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_863716.1
CDS	187..3447
Translation	MASSPDAVISSPPAFLRSGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGCWLNRRQIDGSLNRTPAGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKDAFHEFQKDESRLKEVEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_858623.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_858623.4

ORF Insert Sequence:

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTCGG TTGTGCGCCG TGCAGCAAGT 
CTATTAAGTA AAGTAGTGGA CAGCCTGGCC CCATCCATTA CTAATGTTTT AGTGCAGGGC 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_022411649.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022267357.1 Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe286824
Clone ID Related Accession (Same CDS sequence)	XM_022411649.1
Accession Version	XM_022411649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCAG TTTATGAACC TCTTAAAAGC ATTAATCTTC CGAGACCTGA TAATGCAACT 
CTGTGGGATA AATTGGATCA TTATTACAGA ATTGTCAAGT CAACATTGCT ACTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGAG ATGATCAGAA GGCAAAAATC 
CAGGACAGTC TGTATTGTGC TGCTGGGGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGAAC CCATGAGCTG GAGCACTCTG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTATATGCG TCAGGCCGAT AAGGTCCAGC AGTTTAAGCA GGACCCACGC 
CCAGCAACCT GTCTTCACTC TGTTTTCAAT GTGCACACGG GAGATGAGGT CCTCTCCTAT 
GAAGAATACG GTCATCTTCA GATAAATACA GTGTCACTCT ATCTCCTTTA CCTTGTGGAA 
ATGATTTCTT CAGGACTCCA GATTATCTAT AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGT GTACCCGATT TTGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTA CATTCAAGCT CTGTTGGCTT AGCAAAAGCA 
GCACTAGAAG CGATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCGGTT 
ATATTTGTGG ATCTCGATGC TCACAATCGC AATAGGCAAA CTTTGTGTTC ACTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTCCTAC CCTGCATCAC TTATCCAGCA 
TTTGCCCTAG ATGATGAAGC TTTGTTTAGC CAAACACTTG ATAAAGTGGT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ACAGAACATC ATTGGAAGAT 
CCCAACAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAGTGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGAGTTT TTAGAGGCAA TCCCCAACAA 
GTAAAGGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ATACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AAAATGAGAA AAGAAACCCT 
GGTAGTCAAA AGCGATTTCC TAGCAACTGT GGCCGTGATG GAAAACTCTT TCTTTGGGGA 
CAAGCTCTTT ATATCATTGC AAAACTCCTG GCTGATGAAT TAATCAGTCC TAAAGACATT 
GATCCTGTCC AGCGCTATGT CCCACTACAG AATCAGCGTA ACGTGAGCAT GAGGTTTTCC 
AATCAAGGTC CACTGGAAAA TGATCTCGTA GTTCACGTAG CACTTGTAGC AGAAAGCCAA 
CGCCTTCAGG TTTTCCTCAA CACGTATGGT ATTCAGACTC AAACTCCTCA ACAGGTAGAA 
CCCATTCAGA TTTGGGCTCA GCAGGAGCTG GTGAAAGCTT ATTTCCACCT GGGTATCAAT 
GAAAAATTAG GACTCTCCGG AAGGCCAGAT AGGCCCATTG GCTGCCTCGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTAAGTGAT 
TTCTACATGT CTCAAGATGT TTTACTGCTG ATAGATGACA TAAAGAATGC CCTACAGTTC 
ATTAAGCAAT ACTGGAAAAT GCACGGACGT CCACTTTTCC TTGTCCTCAT CCGGGAAGAC 
AATATACGAG GTAGCCGATT CAACCCCATA TTAGATATGT TGGCAGCCTT TAAAAAAGGA 
ATAGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTA 
GTAGAACAAC TTGACTTCCT ACGAATCAGT GACACAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAC TAGAATTTCC CAAACACTCA AAAGTCAAAC GACAAAGCAG TACCTCAAGT 
GCTCCTGATC TAGAACAGCA ACCAGATGTC GCCATTGCTG AATGGAAGCA CAAGCCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGC AGTTGTCTGG CTAGCCAAGC CATCCTGCTG 
GGTATACTAC TCAAAAGAGA AGGCCCCAAC TTCATCACCA GGGAAGGTAC CGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAGC TTTGGTCGGT TGTGCGCCGT 
GCAGCAAGTC TATTAAGTAA AGTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TTTGGGTGCC TTTGGGCATG AAGAAGAGGT TATCTCTAAT 
CCTTTGTCTC CAAGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCACGATGAG 
AGAGAGGCGG TCATTCAACA AGAACTGGTC ATCCATATTG GCTGGATCAT CTCCAATAAC 
CCTGAGTTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATTATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG CGGAGACAAA CCAGCTAGAG ACTTGTATCA GCTGTCACCT 
AGTGAAGTTA AACAGCTTCT GTTGGATATT CTCCAGCCTC AACAGAATGG AGGATGTTGG 
CTGAACAGGC GTCAGATTGA TGGGTCTTTG AATAGAACTC CTGCTGGGTT CTATGACCGA 
GTATGGCAGA TCCTGGAGCG TACACCCAAT GGTATCATTG TAGCTGGGAA GCATTTGCCA 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAG TACAGACAGA TTGTTGTGGA GTTACTAATG 
GTTGTATCCA TTGTATTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTTGATCTA 
GACAAACTAG TGAAAGATGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
GTTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCACTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTTGTGATG AATTTGCTGC TAGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCATGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022267357.1
CDS	16..3225
Translation	MGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGCWLNRRQIDGSLNRTPAGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKDAFHEFQKDESRLKEVEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_022411649.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022411649.1

ORF Insert Sequence:

ATGGGCTCAG TTTATGAACC TCTTAAAAGC ATTAATCTTC CGAGACCTGA TAATGCAACT 
CTGTGGGATA AATTGGATCA TTATTACAGA ATTGTCAAGT CAACATTGCT ACTGTATCAA 
AGTCCAACCA CAGGTCTCTT TCCCACTAAA ACATGTGGAG ATGATCAGAA GGCAAAAATC 
CAGGACAGTC TGTATTGTGC TGCTGGGGCC TGGGCTTTGG CTCTTGCATA CAGGCGCATT 
GATGATGACA AGGGAAGAAC CCATGAGCTG GAGCACTCTG CTATAAAATG TATGAGAGGA 
ATTCTCTACT GCTATATGCG TCAGGCCGAT AAGGTCCAGC AGTTTAAGCA GGACCCACGC 
CCAGCAACCT GTCTTCACTC TGTTTTCAAT GTGCACACGG GAGATGAGGT CCTCTCCTAT 
GAAGAATACG GTCATCTTCA GATAAATACA GTGTCACTCT ATCTCCTTTA CCTTGTGGAA 
ATGATTTCTT CAGGACTCCA GATTATCTAT AACACTGATG AGGTCTCTTT TATTCAGAAC 
CTTGTATTTT GTGTAGAAAG AGTTTACCGT GTACCCGATT TTGGTGTCTG GGAAAGAGGA 
AGCAAATATA ATAATGGCAG CACAGAGCTA CATTCAAGCT CTGTTGGCTT AGCAAAAGCA 
GCACTAGAAG CGATTAATGG ATTCAACCTC TTTGGCAACC AGGGCTGTTC TTGGTCGGTT 
ATATTTGTGG ATCTCGATGC TCACAATCGC AATAGGCAAA CTTTGTGTTC ACTGTTACCC 
AGAGAATCAA GATCTCATAA CACAGATGCT GCCCTCCTAC CCTGCATCAC TTATCCAGCA 
TTTGCCCTAG ATGATGAAGC TTTGTTTAGC CAAACACTTG ATAAAGTGGT TAGAAAATTA 
AAAGGAAAAT ATGGATTTAA GCGTTTCTTG AGAGATGGAT ACAGAACATC ATTGGAAGAT 
CCCAACAGAC GCTACTACAA ACCAGCTGAA ATTAAGCTGT TTGATGGCAT TGAGTGTGAA 
TTTCCCATAT TTTTCCTTTA CATGATGATT GATGGAGTTT TTAGAGGCAA TCCCCAACAA 
GTAAAGGAGT ATCAGGATCT TCTGACTCCA GTACTTCATC ATACCACAGA AGGTTATCCT 
GTCATACCAA AGTACTATTA TGTGCCAGCT GATTTTGTAG AAAATGAGAA AAGAAACCCT 
GGTAGTCAAA AGCGATTTCC TAGCAACTGT GGCCGTGATG GAAAACTCTT TCTTTGGGGA 
CAAGCTCTTT ATATCATTGC AAAACTCCTG GCTGATGAAT TAATCAGTCC TAAAGACATT 
GATCCTGTCC AGCGCTATGT CCCACTACAG AATCAGCGTA ACGTGAGCAT GAGGTTTTCC 
AATCAAGGTC CACTGGAAAA TGATCTCGTA GTTCACGTAG CACTTGTAGC AGAAAGCCAA 
CGCCTTCAGG TTTTCCTCAA CACGTATGGT ATTCAGACTC AAACTCCTCA ACAGGTAGAA 
CCCATTCAGA TTTGGGCTCA GCAGGAGCTG GTGAAAGCTT ATTTCCACCT GGGTATCAAT 
GAAAAATTAG GACTCTCCGG AAGGCCAGAT AGGCCCATTG GCTGCCTCGG TACATCAAAG 
ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCTA TCATCTTTGA CCTAAGTGAT 
TTCTACATGT CTCAAGATGT TTTACTGCTG ATAGATGACA TAAAGAATGC CCTACAGTTC 
ATTAAGCAAT ACTGGAAAAT GCACGGACGT CCACTTTTCC TTGTCCTCAT CCGGGAAGAC 
AATATACGAG GTAGCCGATT CAACCCCATA TTAGATATGT TGGCAGCCTT TAAAAAAGGA 
ATAGTTGGAG GAGTCAAAGT TCATGTTGAT CGTCTACAGA CACTAATATC TGGAGCTGTA 
GTAGAACAAC TTGACTTCCT ACGAATCAGT GACACAGAAG AGCTTCCAGA ATTTAAGAGT 
TTTGAGGAAC TAGAATTTCC CAAACACTCA AAAGTCAAAC GACAAAGCAG TACCTCAAGT 
GCTCCTGATC TAGAACAGCA ACCAGATGTC GCCATTGCTG AATGGAAGCA CAAGCCCACC 
CATGAAATTC TTCAAAAACT GAATGACTGC AGTTGTCTGG CTAGCCAAGC CATCCTGCTG 
GGTATACTAC TCAAAAGAGA AGGCCCCAAC TTCATCACCA GGGAAGGTAC CGTTTCTGAT 
CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCAAAAAGC TTTGGTCGGT TGTGCGCCGT 
GCAGCAAGTC TATTAAGTAA AGTAGTGGAC AGCCTGGCCC CATCCATTAC TAATGTTTTA 
GTGCAGGGCA AACAGGTAAC TTTGGGTGCC TTTGGGCATG AAGAAGAGGT TATCTCTAAT 
CCTTTGTCTC CAAGAGTGAT TAAGAACATC ATCTATTACA AGTGTAATAC CCACGATGAG 
AGAGAGGCGG TCATTCAACA AGAACTGGTC ATCCATATTG GCTGGATCAT CTCCAATAAC 
CCTGAGTTAT TCAGTGGCAT GCTGAAAATA CGAATTGGGT GGATTATCCA TGCTATGGAG 
TATGAACTAC AGATCCGTGG CGGAGACAAA CCAGCTAGAG ACTTGTATCA GCTGTCACCT 
AGTGAAGTTA AACAGCTTCT GTTGGATATT CTCCAGCCTC AACAGAATGG AGGATGTTGG 
CTGAACAGGC GTCAGATTGA TGGGTCTTTG AATAGAACTC CTGCTGGGTT CTATGACCGA 
GTATGGCAGA TCCTGGAGCG TACACCCAAT GGTATCATTG TAGCTGGGAA GCATTTGCCA 
CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA ATTTCTCTCT CCTTGTTGAA 
GACATGTTGG GAAATATTGA CCAGCCAAAG TACAGACAGA TTGTTGTGGA GTTACTAATG 
GTTGTATCCA TTGTATTGGA AAGAAACCCT GAGCTAGAAT TTCAAGACAA AGTTGATCTA 
GACAAACTAG TGAAAGATGC ATTTCATGAA TTTCAAAAAG ATGAGAGTCG ACTAAAGGAA 
GTTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC CCCCACTGGG AAAAAGAGGA 
ACATGCAGCT ATTTGACAAA GGTTGTGATG AATTTGCTGC TAGAAGGAGA AGTCAAGCCA 
AGCAATGATG ACCCATGTCT GGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_014109653.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965128.1 Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.

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CloneID	OCe35416
Clone ID Related Accession (Same CDS sequence)	XM_014109653.1 , XM_014109653.2
Accession Version	XM_014109653.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTTGG CGGTACGCTA CGGGGCTGCC 
TTTACCCAGA AATTTTCTTC CTCTATAGCT CCACACATTA CTACTTTTCT GGTCCATGGG 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965128.1
CDS	144..3404
Translation	MASSPDAVISSPPAFLRSGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGCWLNRRQIDGSLNRTPAGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKDAFHEFQKDESRLKEVEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_014109653.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphorylase kinase, beta (PHKB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109653.1

ORF Insert Sequence:

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTTGG CGGTACGCTA CGGGGCTGCC 
TTTACCCAGA AATTTTCTTC CTCTATAGCT CCACACATTA CTACTTTTCT GGTCCATGGG 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKB ( XM_014109653.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965128.1 Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.

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CloneID	OCe35416
Clone ID Related Accession (Same CDS sequence)	XM_014109653.1 , XM_014109653.2
Accession Version	XM_014109653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTTGG CGGTACGCTA CGGGGCTGCC 
TTTACCCAGA AATTTTCTTC CTCTATAGCT CCACACATTA CTACTTTTCT GGTCCATGGG 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965128.1
CDS	187..3447
Translation	MASSPDAVISSPPAFLRSGSVYEPLKSINLPRPDNATLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGDDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPATCLHSVFNVHTGDEVLSYEEYGHLQINTVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRRYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPQQVKEYQDLLTPVLHHTTEGYPVIPKYYYVPADFVENEKRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLQNQRNVSMRFSNQGPLENDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWAQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRILGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDMLAAFKKGIVGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEFPKHSKVKRQSSTSSAPDLEQQPDVAIAEWKHKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITREGTVSDHIERVYRRAGSKKLWLAVRYGAAFTQKFSSSIAPHITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPARDLYQLSPSEVKQLLLDILQPQQNGGCWLNRRQIDGSLNRTPAGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLGNIDQPKYRQIVVELLMVVSIVLERNPELEFQDKVDLDKLVKDAFHEFQKDESRLKEVEKQDDMTSFYNTPPLGKRGTCSYLTKVVMNLLLEGEVKPSNDDPCLVS

Target ORF information:

RefSeq Version	XM_014109653.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphorylase kinase regulatory subunit beta (PHKB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109653.2

ORF Insert Sequence:

ATGGCCAGCT CACCTGATGC CGTCATCTCT TCTCCTCCAG CTTTCTTAAG GTCTGGCTCA 
GTTTATGAAC CTCTTAAAAG CATTAATCTT CCGAGACCTG ATAATGCAAC TCTGTGGGAT 
AAATTGGATC ATTATTACAG AATTGTCAAG TCAACATTGC TACTGTATCA AAGTCCAACC 
ACAGGTCTCT TTCCCACTAA AACATGTGGA GATGATCAGA AGGCAAAAAT CCAGGACAGT 
CTGTATTGTG CTGCTGGGGC CTGGGCTTTG GCTCTTGCAT ACAGGCGCAT TGATGATGAC 
AAGGGAAGAA CCCATGAGCT GGAGCACTCT GCTATAAAAT GTATGAGAGG AATTCTCTAC 
TGCTATATGC GTCAGGCCGA TAAGGTCCAG CAGTTTAAGC AGGACCCACG CCCAGCAACC 
TGTCTTCACT CTGTTTTCAA TGTGCACACG GGAGATGAGG TCCTCTCCTA TGAAGAATAC 
GGTCATCTTC AGATAAATAC AGTGTCACTC TATCTCCTTT ACCTTGTGGA AATGATTTCT 
TCAGGACTCC AGATTATCTA TAACACTGAT GAGGTCTCTT TTATTCAGAA CCTTGTATTT 
TGTGTAGAAA GAGTTTACCG TGTACCCGAT TTTGGTGTCT GGGAAAGAGG AAGCAAATAT 
AATAATGGCA GCACAGAGCT ACATTCAAGC TCTGTTGGCT TAGCAAAAGC AGCACTAGAA 
GCGATTAATG GATTCAACCT CTTTGGCAAC CAGGGCTGTT CTTGGTCGGT TATATTTGTG 
GATCTCGATG CTCACAATCG CAATAGGCAA ACTTTGTGTT CACTGTTACC CAGAGAATCA 
AGATCTCATA ACACAGATGC TGCCCTCCTA CCCTGCATCA CTTATCCAGC ATTTGCCCTA 
GATGATGAAG CTTTGTTTAG CCAAACACTT GATAAAGTGG TTAGAAAATT AAAAGGAAAA 
TATGGATTTA AGCGTTTCTT GAGAGATGGA TACAGAACAT CATTGGAAGA TCCCAACAGA 
CGCTACTACA AACCAGCTGA AATTAAGCTG TTTGATGGCA TTGAGTGTGA ATTTCCCATA 
TTTTTCCTTT ACATGATGAT TGATGGAGTT TTTAGAGGCA ATCCCCAACA AGTAAAGGAG 
TATCAGGATC TTCTGACTCC AGTACTTCAT CATACCACAG AAGGTTATCC TGTCATACCA 
AAGTACTATT ATGTGCCAGC TGATTTTGTA GAAAATGAGA AAAGAAACCC TGGTAGTCAA 
AAGCGATTTC CTAGCAACTG TGGCCGTGAT GGAAAACTCT TTCTTTGGGG ACAAGCTCTT 
TATATCATTG CAAAACTCCT GGCTGATGAA TTAATCAGTC CTAAAGACAT TGATCCTGTC 
CAGCGCTATG TCCCACTACA GAATCAGCGT AACGTGAGCA TGAGGTTTTC CAATCAAGGT 
CCACTGGAAA ATGATCTCGT AGTTCACGTA GCACTTGTAG CAGAAAGCCA ACGCCTTCAG 
GTTTTCCTCA ACACGTATGG TATTCAGACT CAAACTCCTC AACAGGTAGA ACCCATTCAG 
ATTTGGGCTC AGCAGGAGCT GGTGAAAGCT TATTTCCACC TGGGTATCAA TGAAAAATTA 
GGACTCTCCG GAAGGCCAGA TAGGCCCATT GGCTGCCTCG GTACATCAAA GATTTATCGC 
ATTCTAGGAA AGACTGTGGT TTGTTACCCT ATCATCTTTG ACCTAAGTGA TTTCTACATG 
TCTCAAGATG TTTTACTGCT GATAGATGAC ATAAAGAATG CCCTACAGTT CATTAAGCAA 
TACTGGAAAA TGCACGGACG TCCACTTTTC CTTGTCCTCA TCCGGGAAGA CAATATACGA 
GGTAGCCGAT TCAACCCCAT ATTAGATATG TTGGCAGCCT TTAAAAAAGG AATAGTTGGA 
GGAGTCAAAG TTCATGTTGA TCGTCTACAG ACACTAATAT CTGGAGCTGT AGTAGAACAA 
CTTGACTTCC TACGAATCAG TGACACAGAA GAGCTTCCAG AATTTAAGAG TTTTGAGGAA 
CTAGAATTTC CCAAACACTC AAAAGTCAAA CGACAAAGCA GTACCTCAAG TGCTCCTGAT 
CTAGAACAGC AACCAGATGT CGCCATTGCT GAATGGAAGC ACAAGCCCAC CCATGAAATT 
CTTCAAAAAC TGAATGACTG CAGTTGTCTG GCTAGCCAAG CCATCCTGCT GGGTATACTA 
CTCAAAAGAG AAGGCCCCAA CTTCATCACC AGGGAAGGTA CCGTTTCTGA TCACATTGAG 
AGAGTCTATA GAAGAGCTGG CAGCAAAAAG CTTTGGTTGG CGGTACGCTA CGGGGCTGCC 
TTTACCCAGA AATTTTCTTC CTCTATAGCT CCACACATTA CTACTTTTCT GGTCCATGGG 
AAACAGGTAA CTTTGGGTGC CTTTGGGCAT GAAGAAGAGG TTATCTCTAA TCCTTTGTCT 
CCAAGAGTGA TTAAGAACAT CATCTATTAC AAGTGTAATA CCCACGATGA GAGAGAGGCG 
GTCATTCAAC AAGAACTGGT CATCCATATT GGCTGGATCA TCTCCAATAA CCCTGAGTTA 
TTCAGTGGCA TGCTGAAAAT ACGAATTGGG TGGATTATCC ATGCTATGGA GTATGAACTA 
CAGATCCGTG GCGGAGACAA ACCAGCTAGA GACTTGTATC AGCTGTCACC TAGTGAAGTT 
AAACAGCTTC TGTTGGATAT TCTCCAGCCT CAACAGAATG GAGGATGTTG GCTGAACAGG 
CGTCAGATTG ATGGGTCTTT GAATAGAACT CCTGCTGGGT TCTATGACCG AGTATGGCAG 
ATCCTGGAGC GTACACCCAA TGGTATCATT GTAGCTGGGA AGCATTTGCC ACAGCAACCA 
ACCCTGTCAG ATATGACCAT GTATGAGATG AATTTCTCTC TCCTTGTTGA AGACATGTTG 
GGAAATATTG ACCAGCCAAA GTACAGACAG ATTGTTGTGG AGTTACTAAT GGTTGTATCC 
ATTGTATTGG AAAGAAACCC TGAGCTAGAA TTTCAAGACA AAGTTGATCT AGACAAACTA 
GTGAAAGATG CATTTCATGA ATTTCAAAAA GATGAGAGTC GACTAAAGGA AGTTGAAAAA 
CAAGATGACA TGACTTCCTT TTACAACACT CCCCCACTGG GAAAAAGAGG AACATGCAGC 
TATTTGACAA AGGTTGTGAT GAATTTGCTG CTAGAAGGAG AAGTCAAGCC AAGCAATGAT 
GACCCATGTC TGGTTAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PHKB cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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