Mcf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcf2
Entrez Gene ID	317598
Full Name	MCF.2 cell line derived transforming sequence
Synonyms	RGD1566098
General protein information	Preferred Names MCF.2 cell line derived transforming sequence Names MCF.2 cell line derived transforming sequence proto-oncogene DBL mcf.2 transforming sequence proto-oncogene DBL-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq36

mRNA and Protein(s)

mRNA	Protein	Name
XM_008773576.2	XP_008771798.1	proto-oncogene DBL isoform X1
XM_008758578.2	XP_008756800.2	proto-oncogene DBL isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MCF2	XP_003954149.1
Xenopus tropicalis (tropical clawed frog)	mcf2	XP_002932674.2
Mus musculus (house mouse)	Mcf2	NP_573460.2
Gallus gallus (chicken)	MCF2	XP_420239.4
Danio rerio (zebrafish)	mcf2a	XP_005173283.1
Homo sapiens (human)	MCF2	NP_001165347.1
Canis lupus familiaris (dog)	MCF2	XP_005641937.1
Rattus norvegicus (Norway rat)	Mcf2	XP_006257657.1
Macaca mulatta (Rhesus monkey)	MCF2	XP_002808597.1
Bos taurus (cattle)	MCF2	XP_005227614.1

Gene Ontology

The following Mcf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa47624	XM_008773576.2 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56129	XM_008758578.2 Latest version!	Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcf2 ( XM_008773576.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771798.1 Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47624
Clone ID Related Accession (Same CDS sequence)	XM_008773576.2
Accession Version	XM_008773576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	proto-oncogene DBL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAG CAAGGTCCCC TCCAACAGGG CCCTCTCCAG CAGGGCCCCC TCCAGCAGGA 
TCCCCTCCGG CTCCCGCCCT TGCCTCGCCG TGGCTGGCGT TCAGGAACTT GCCTAAGGCT 
GCCTCCCGGG CGCCCAGGAC TAGGAAACGG CAGCTAAATC CAGACAACGG TACTTTCGTG 
CCTTTCACGC AGACGATCCC GGTGCAGGTG GACATGGCCA TGGCCCTGCA TCGCGTTAGG 
ATCGCAGAGA AGTATGGGAA GAACTACCTC TGCTTGCTGC AGGCAGTCTT CCTCGCAGAA 
ACTGGGCTGC TCCTCCAGTT AAAGGATATC AATCATTTCC TAATGCAAGA CATTGCCTTC 
CTGTCTGGTG GCCGGGGAAG GGACAATGCT TGGATCATTA CGTTTCCAGA AAACTGTAAC 
TTCAGATGCA TACCAGAGGA AGTCATTGCA AAAGTGCTCA ATTACTTGAC ATCTATTGCA 
AGACAAAGTG GATCTGACTC CCGATTTACT ATTATTCTGG ATCGAAGAAT GGATACATGG 
TCTTCCCTCA AAATTTCCCT CCAGAAAATC TCAGCCTCTT TCCCTGGGAA CTTACACTTA 
GTCCTGGTTT TACGTCCTAC CAGTTTTCTT CAACGGACTT TCACAGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTAT GCTCAAATTA CCAGTTGTTA TGCTGAGCTC AGTTAGTGAT 
TTGCTGACAT ACATTGATGA CAAGCAGTTA ACCCCTGAGT TAGGCGGCAC CTTGCAGTAC 
TGCCACAGTG AATGGATCAT CTTCAGAAAT GCCATAGAGA AATTTGCTGT CACAGTGAAA 
GAAATGGCTC AGATGTTGCA ATCCTTTGGA ACAGAACTGG CTGAAACAGA GCTACCAGAT 
GATATCCCTT CAATAGAAGA GATTCTGGCA GGTCGTGCTG AAAGGTATCA TCTATTGAAG 
AATGACTTAA CTACTGTAAC CAAAGAAGGA AAAGTTCTTC TCATGAACCT GCAAGTGCCT 
GACACTGAAG AAAATCTCAG TTCAAGTCAT GAATGTCCCC AGCATATAAA TGGTGACTGG 
CAAACTATTA AAAAGTTGCT GGCTCAGGTG CATGATATGG AAACTGCTTT TGATGGATTT 
TGGGAAAAAC ATCAGCTGAA AATGGAGCAA TATCTGCAAC TGTGGAAGTT TGAGCAAGAA 
TTTCAAGAGG TTGTGACTCA AGTTGAATTT TTATTAAGTC AACAGAGAGA ACTGGGTGAT 
GTAACGGGGA ACTTAGCTCA AGTAAAACAA AGACTTAAAA AAATAGAGAT CTTGGATGAC 
AAATCTCAGG AACTGTTAGC CAAGGGCCGG ATTGTGATAT TGCATGGCCA CAAGCTGGCA 
TCGAATCACC ACTATGCCCT TGATTTAATC TGCCAGAGGT GTAATGAGCT ACGCTACCTT 
TCTGATATTT TAGTTAATGA GATCAGATCA AAGCGGATCC AGCTGAGTAG GACTTTCAAA 
ATGCACAGAC TTCTACAGCA GGCACGTCAG TGCTGTGATC AAGGGGAATG CCTCCTGGCT 
AACCGGGGAA TGGATAAGTT TCAGACTAAA GAAGATGCTC AGAAAGCCCT TCAGGATGTG 
GACAATTTCC TTCAAATGGC CATGCCTTTC ATCAATTATG ATATCGAAAA CCTGCAATTT 
GAATTTGATG TGCTCCTATC TCCTGAACTC AAGGCTCAAA TGAAGAATAT ACAACGAAAG 
CTTGAAAATA TTCGAAGTGT ATTTGAGAAT CAACAGAATG CTTATAAGAT CTCAAAAGAT 
AGACCTGAGG GGGCAGTCCA GCGTATGATT CCGGCACCTG AAAGTGTGAT GAGAGCCAGA 
ACAATATTCT TTTCACCTAA ACATGTTGGA ATTGGATATT CTTTCTTTCA AGCATGTAAA 
ATTTTTTCAA AAGTGAAAAA GTCTTGGAGA CAGATTCAAG CTCAGAGCAA TGTGAAAGTC 
CAAGCCACTC AGGATAGCCA AGAGAAGAAT TTTGATCAGC CTCCCAAATT GGACAGTAGC 
TTGGATATTT TAAAGAACCA TGTTCTGAAT GAACTGATCC AGACAGAGAG GGCCTATGTT 
CGAGAGCTGT TTACTGTTTT GTTGGGCTAC CGATCTGAGA TGGACAATCC ACAGATGTTT 
GATCTTATGC CACCTCTCCT GAGAAATAAA AAGGATGTTC TCTTTGGAAA TATGGCAGAA 
ATATATGAAT TTCATAACAA CATTTTCATG AGCAGACTGG AAGATTGCTC TGATGCTCCA 
GAAAGAGTGG GACCTTGCTT CCTGGAAAGG AAGGAAGATT TTCAGATGTA TGCCAAATAC 
TGTCAGAATA AACCCAGGTC AGAGTTAATT TGGAGGAAGT ATTCTGAATG TGCCTTTTTC 
CAGGAATGTC AACGGAAATT GAAACATCGA CTTGGTCTGG ATTCCTATTT GCTCAAGCCA 
GTTCAACGAA TCACTAAGTA TCAGTTGCTT TTAAAGGAGC TTTTAAAGTA TAGCAAAGAA 
GGTGAAGGAA CTACTAAATT GAAGGAGGCC CTTGACTCAA TGCTGGAGCT GCTCAAGTCA 
GTGAATGATT CCATGCATCA GACTGCCATC AATGGCTATG TTGGAAATAT GAATGATCTG 
GGAAAGATGA TTCTTCAAGG TGCCTTCAGT GTTTGGGTAG GACATCGGAA AGGTGCTACC 
AAGATGAAGG ATTTTGCCCG GTTCAAACCC ATGCAGAGAC ACCTTTTCTT GTATGAAAAA 
GCTGTTATGT TTTGTAAGAG GCGTTTCGAA TCTGGAGAAG GTGCTGACAG ATACCCATCA 
TACAGTTTTA AGCACTGCCT AAAAATGGAA GAAGCTGGTA TCACAGAGCA TGTGAAAGGC 
GATAACCGAA AATTTGAAAT ATGGTATGGT GAGAAGGAAG AAATTTATAT TGTTCAGGCT 
CCAAATATTG ATGTGAAGAT GTTATGGCTA AAAGAAATAA GAAACATTTT GTTGAAGCAG 
CAGGAACTTT TGACTGCTAA AACCCAGCAG GATCAGTTAA GCGACCAGGA CCAACTTCCT 
CCCCAGCAGC AGAATGCTGA AAAGCAAGAA GAAACTCTTT TAAATGCTGT GGAAGCAGGA 
GCAGAAGTGG AACAAGCGAG TGCCATGATG GAGGTCAGGG AGGCAGCTCT GCTAGTTCAG 
GCAGAAGCAA ATTGCACAAC TTGGAATGGG ATGCTGCCAT GTACAGAAGG CACCGCTGCC 
ACTGCTGAGC ATCCATGCAA CTCTCTCTCC CCTAACAGCG ACAATGGAGA AGACAGGTCA 
CAAATGAGAT CCATGTCGGA GGTTCAGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771798.1
CDS	380..3712
Translation	MAEARSPPTGPSPAGPPPAGSPPAPALASPWLAFRNLPKAASRAPRTRKRQLNPDNGTFVPFTQTIPVQVDMAMALHRVRIAEKYGKNYLCLLQAVFLAETGLLLQLKDINHFLMQDIAFLSGGRGRDNAWIITFPENCNFRCIPEEVIAKVLNYLTSIARQSGSDSRFTIILDRRMDTWSSLKISLQKISASFPGNLHLVLVLRPTSFLQRTFTDIGFRFSQEDFMLKLPVVMLSSVSDLLTYIDDKQLTPELGGTLQYCHSEWIIFRNAIEKFAVTVKEMAQMLQSFGTELAETELPDDIPSIEEILAGRAERYHLLKNDLTTVTKEGKVLLMNLQVPDTEENLSSSHECPQHINGDWQTIKKLLAQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQEFQEVVTQVEFLLSQQRELGDVTGNLAQVKQRLKKIEILDDKSQELLAKGRIVILHGHKLASNHHYALDLICQRCNELRYLSDILVNEIRSKRIQLSRTFKMHRLLQQARQCCDQGECLLANRGMDKFQTKEDAQKALQDVDNFLQMAMPFINYDIENLQFEFDVLLSPELKAQMKNIQRKLENIRSVFENQQNAYKISKDRPEGAVQRMIPAPESVMRARTIFFSPKHVGIGYSFFQACKIFSKVKKSWRQIQAQSNVKVQATQDSQEKNFDQPPKLDSSLDILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIFMSRLEDCSDAPERVGPCFLERKEDFQMYAKYCQNKPRSELIWRKYSECAFFQECQRKLKHRLGLDSYLLKPVQRITKYQLLLKELLKYSKEGEGTTKLKEALDSMLELLKSVNDSMHQTAINGYVGNMNDLGKMILQGAFSVWVGHRKGATKMKDFARFKPMQRHLFLYEKAVMFCKRRFESGEGADRYPSYSFKHCLKMEEAGITEHVKGDNRKFEIWYGEKEEIYIVQAPNIDVKMLWLKEIRNILLKQQELLTAKTQQDQLSDQDQLPPQQQNAEKQEETLLNAVEAGAEVEQASAMMEVREAALLVQAEANCTTWNGMLPCTEGTAATAEHPCNSLSPNSDNGEDRSQMRSMSEVQF

Target ORF information:

RefSeq Version	XM_008773576.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773576.2

ORF Insert Sequence:

ATGGCGGAAG CAAGGTCCCC TCCAACAGGG CCCTCTCCAG CAGGGCCCCC TCCAGCAGGA 
TCCCCTCCGG CTCCCGCCCT TGCCTCGCCG TGGCTGGCGT TCAGGAACTT GCCTAAGGCT 
GCCTCCCGGG CGCCCAGGAC TAGGAAACGG CAGCTAAATC CAGACAACGG TACTTTCGTG 
CCTTTCACGC AGACGATCCC GGTGCAGGTG GACATGGCCA TGGCCCTGCA TCGCGTTAGG 
ATCGCAGAGA AGTATGGGAA GAACTACCTC TGCTTGCTGC AGGCAGTCTT CCTCGCAGAA 
ACTGGGCTGC TCCTCCAGTT AAAGGATATC AATCATTTCC TAATGCAAGA CATTGCCTTC 
CTGTCTGGTG GCCGGGGAAG GGACAATGCT TGGATCATTA CGTTTCCAGA AAACTGTAAC 
TTCAGATGCA TACCAGAGGA AGTCATTGCA AAAGTGCTCA ATTACTTGAC ATCTATTGCA 
AGACAAAGTG GATCTGACTC CCGATTTACT ATTATTCTGG ATCGAAGAAT GGATACATGG 
TCTTCCCTCA AAATTTCCCT CCAGAAAATC TCAGCCTCTT TCCCTGGGAA CTTACACTTA 
GTCCTGGTTT TACGTCCTAC CAGTTTTCTT CAACGGACTT TCACAGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTAT GCTCAAATTA CCAGTTGTTA TGCTGAGCTC AGTTAGTGAT 
TTGCTGACAT ACATTGATGA CAAGCAGTTA ACCCCTGAGT TAGGCGGCAC CTTGCAGTAC 
TGCCACAGTG AATGGATCAT CTTCAGAAAT GCCATAGAGA AATTTGCTGT CACAGTGAAA 
GAAATGGCTC AGATGTTGCA ATCCTTTGGA ACAGAACTGG CTGAAACAGA GCTACCAGAT 
GATATCCCTT CAATAGAAGA GATTCTGGCA GGTCGTGCTG AAAGGTATCA TCTATTGAAG 
AATGACTTAA CTACTGTAAC CAAAGAAGGA AAAGTTCTTC TCATGAACCT GCAAGTGCCT 
GACACTGAAG AAAATCTCAG TTCAAGTCAT GAATGTCCCC AGCATATAAA TGGTGACTGG 
CAAACTATTA AAAAGTTGCT GGCTCAGGTG CATGATATGG AAACTGCTTT TGATGGATTT 
TGGGAAAAAC ATCAGCTGAA AATGGAGCAA TATCTGCAAC TGTGGAAGTT TGAGCAAGAA 
TTTCAAGAGG TTGTGACTCA AGTTGAATTT TTATTAAGTC AACAGAGAGA ACTGGGTGAT 
GTAACGGGGA ACTTAGCTCA AGTAAAACAA AGACTTAAAA AAATAGAGAT CTTGGATGAC 
AAATCTCAGG AACTGTTAGC CAAGGGCCGG ATTGTGATAT TGCATGGCCA CAAGCTGGCA 
TCGAATCACC ACTATGCCCT TGATTTAATC TGCCAGAGGT GTAATGAGCT ACGCTACCTT 
TCTGATATTT TAGTTAATGA GATCAGATCA AAGCGGATCC AGCTGAGTAG GACTTTCAAA 
ATGCACAGAC TTCTACAGCA GGCACGTCAG TGCTGTGATC AAGGGGAATG CCTCCTGGCT 
AACCGGGGAA TGGATAAGTT TCAGACTAAA GAAGATGCTC AGAAAGCCCT TCAGGATGTG 
GACAATTTCC TTCAAATGGC CATGCCTTTC ATCAATTATG ATATCGAAAA CCTGCAATTT 
GAATTTGATG TGCTCCTATC TCCTGAACTC AAGGCTCAAA TGAAGAATAT ACAACGAAAG 
CTTGAAAATA TTCGAAGTGT ATTTGAGAAT CAACAGAATG CTTATAAGAT CTCAAAAGAT 
AGACCTGAGG GGGCAGTCCA GCGTATGATT CCGGCACCTG AAAGTGTGAT GAGAGCCAGA 
ACAATATTCT TTTCACCTAA ACATGTTGGA ATTGGATATT CTTTCTTTCA AGCATGTAAA 
ATTTTTTCAA AAGTGAAAAA GTCTTGGAGA CAGATTCAAG CTCAGAGCAA TGTGAAAGTC 
CAAGCCACTC AGGATAGCCA AGAGAAGAAT TTTGATCAGC CTCCCAAATT GGACAGTAGC 
TTGGATATTT TAAAGAACCA TGTTCTGAAT GAACTGATCC AGACAGAGAG GGCCTATGTT 
CGAGAGCTGT TTACTGTTTT GTTGGGCTAC CGATCTGAGA TGGACAATCC ACAGATGTTT 
GATCTTATGC CACCTCTCCT GAGAAATAAA AAGGATGTTC TCTTTGGAAA TATGGCAGAA 
ATATATGAAT TTCATAACAA CATTTTCATG AGCAGACTGG AAGATTGCTC TGATGCTCCA 
GAAAGAGTGG GACCTTGCTT CCTGGAAAGG AAGGAAGATT TTCAGATGTA TGCCAAATAC 
TGTCAGAATA AACCCAGGTC AGAGTTAATT TGGAGGAAGT ATTCTGAATG TGCCTTTTTC 
CAGGAATGTC AACGGAAATT GAAACATCGA CTTGGTCTGG ATTCCTATTT GCTCAAGCCA 
GTTCAACGAA TCACTAAGTA TCAGTTGCTT TTAAAGGAGC TTTTAAAGTA TAGCAAAGAA 
GGTGAAGGAA CTACTAAATT GAAGGAGGCC CTTGACTCAA TGCTGGAGCT GCTCAAGTCA 
GTGAATGATT CCATGCATCA GACTGCCATC AATGGCTATG TTGGAAATAT GAATGATCTG 
GGAAAGATGA TTCTTCAAGG TGCCTTCAGT GTTTGGGTAG GACATCGGAA AGGTGCTACC 
AAGATGAAGG ATTTTGCCCG GTTCAAACCC ATGCAGAGAC ACCTTTTCTT GTATGAAAAA 
GCTGTTATGT TTTGTAAGAG GCGTTTCGAA TCTGGAGAAG GTGCTGACAG ATACCCATCA 
TACAGTTTTA AGCACTGCCT AAAAATGGAA GAAGCTGGTA TCACAGAGCA TGTGAAAGGC 
GATAACCGAA AATTTGAAAT ATGGTATGGT GAGAAGGAAG AAATTTATAT TGTTCAGGCT 
CCAAATATTG ATGTGAAGAT GTTATGGCTA AAAGAAATAA GAAACATTTT GTTGAAGCAG 
CAGGAACTTT TGACTGCTAA AACCCAGCAG GATCAGTTAA GCGACCAGGA CCAACTTCCT 
CCCCAGCAGC AGAATGCTGA AAAGCAAGAA GAAACTCTTT TAAATGCTGT GGAAGCAGGA 
GCAGAAGTGG AACAAGCGAG TGCCATGATG GAGGTCAGGG AGGCAGCTCT GCTAGTTCAG 
GCAGAAGCAA ATTGCACAAC TTGGAATGGG ATGCTGCCAT GTACAGAAGG CACCGCTGCC 
ACTGCTGAGC ATCCATGCAA CTCTCTCTCC CCTAACAGCG ACAATGGAGA AGACAGGTCA 
CAAATGAGAT CCATGTCGGA GGTTCAGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mcf2 ( XM_008758578.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756800.2 Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa56129
Clone ID Related Accession (Same CDS sequence)	XM_008758578.2
Accession Version	XM_008758578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	proto-oncogene DBL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGCGGAAG CAAGGTCCCC TCCAACAGGG CCCTCTCCAG CAGGGCCCCC TCCAGCAGGA 
TCCCCTCCGG CTCCCGCCCT TGCCTCGCCG TGGCTGGCGT TCAGGAACTT GCCTAAGGCT 
GCCTCCCGGG CGCCCAGGAC TAGGAAACGG CAGCTAAATC CAGACAACGG TACTTTCGTG 
CCTTTCACGC AGACGATCCC GGTGCAGGTG GACATGGCCA TGGCCCTGCA TCGCGTTAGG 
ATCGCAGAGA AGTATGGGAA GAACTACCTC TGCTTGCTGC AGGCAGTCTT CCTCGCAGAA 
ACTGGGCTGC TCCTCCAGTT AAAGGATATC AATCATTTCC TAATGCAAGA CATTGCCTTC 
CTGTCTGGTG GCCGGGGAAG GGACAATGCT TGGATCATTA CGTTTCCAGA AAACTGTAAC 
TTCAGATGCA TACCAGAGGA AGTCATTGCA AAAGTGCTCA ATTACTTGAC ATCTATTGCA 
AGACAAAGTG GATCTGACTC CCGATTTACT ATTATTCTGG ATCGAAGAAT GGATACATGG 
TCTTCCCTCA AAATTTCCCT CCAGAAAATC TCAGCCTCTT TCCCTGGGAA CTTACACTTA 
GTCCTGGTTT TACGTCCTAC CAGTTTTCTT CAACGGACTT TCACAGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTAT GCTCAAATTA CCAGTTGTTA TGCTGAGCTC AGTTAGTGAT 
TTGCTGACAT ACATTGATGA CAAGCAGTTA ACCCCTGAGT TAGGCGGCAC CTTGCAGTAC 
TGCCACAGTG AATGGATCAT CTTCAGAAAT GTATGTGTGT CTGGCCTTAC TGTAGAGCCT 
ATCCTTCGTA GGCACTTTTG CCAACTCAAC CACCTCCTTG GCTATAGAGT GCCACACTTG 
CAGATTCTTG GATCCTGGAA TACTAATGAC TTAACTACTG TAACCAAAGA AGGAAAAGTT 
CTTCTCATGA ACCTGCAAGT GCCTGACACT GAAGAAAATC TCAGTTCAAG TCATGAATGT 
CCCCAGCATA TAAATGGTGA CTGGCAAACT ATTAAAAAGT TGCTGGCTCA GGTGCATGAT 
ATGGAAACTG CTTTTGATGG ATTTTGGGAA AAACATCAGC TGAAAATGGA GCAATATCTG 
CAACTGTGGA AGTTTGAGCA AGAATTTCAA GAGGTTGTGA CTCAAGTTGA ATTTTTATTA 
AGTCAACAGA GAGAACTGGG TGATGTAACG GGGAACTTAG CTCAAGTAAA ACAAAGACTT 
AAAAAAATAG AGATCTTGGA TGACAAATCT CAGGAACTGT TAGCCAAGGG CCGGATTGTG 
ATATTGCATG GCCACAAGCT GGCATCGAAT CACCACTATG CCCTTGATTT AATCTGCCAG 
AGGTGTAATG AGCTACGCTA CCTTTCTGAT ATTTTAGTTA ATGAGATCAG ATCAAAGCGG 
ATCCAGCTGA GTAGGACTTT CAAAATGCAC AGACTTCTAC AGCAGGCACG TCAGTGCTGT 
GATCAAGGGG AATGCCTCCT GGCTAACCGG GGAATGGATA AGTTTCAGAC TAAAGAAGAT 
GCTCAGAAAG CCCTTCAGGA TGTGGACAAT TTCCTTCAAA TGGCCATGCC TTTCATCAAT 
TATGATATCG AAAACCTGCA ATTTGAATTT GATGTGCTCC TATCTCCTGA ACTCAAGGCT 
CAAATGAAGA ATATACAACG AAAGCTTGAA AATATTCGAA GTGTATTTGA GAATCAACAG 
AATGCTTATA AGATCTCAAA AGATAGACCT GAGGGGGCAG TCCAGCGTAT GATTCCGGCA 
CCTGAAAGTG TGATGAGAGC CAGAACAATA TTCTTTTCAC CTAAACATGT TGGAATTGGA 
TATTCTTTCT TTCAAGCATG TAAAATTTTT TCAAAAGTGA AAAAGTCTTG GAGACAGATT 
CAAGCTCAGA GCAATGTGAA AGTCCAAGCC ACTCAGGATA GCCAAGAGAA GAATTTTGAT 
CAGCCTCCCA AATTGGACAG TAGCTTGGAT ATTTTAAAGA ACCATGTTCT GAATGAACTG 
ATCCAGACAG AGAGGGCCTA TGTTCGAGAG CTGTTTACTG TTTTGTTGGG CTACCGATCT 
GAGATGGACA ATCCACAGAT GTTTGATCTT ATGCCACCTC TCCTGAGAAA TAAAAAGGAT 
GTTCTCTTTG GAAATATGGC AGAAATATAT GAATTTCATA ACAACATTTT CATGAGCAGA 
CTGGAAGATT GCTCTGATGC TCCAGAAAGA GTGGGACCTT GCTTCCTGGA AAGGAAGGAA 
GATTTTCAGA TGTATGCCAA ATACTGTCAG AATAAACCCA GGTCAGAGTT AATTTGGAGG 
AAGTATTCTG AATGTGCCTT TTTCCAGGAA TGTCAACGGA AATTGAAACA TCGACTTGGT 
CTGGATTCCT ATTTGCTCAA GCCAGTTCAA CGAATCACTA AGTATCAGTT GCTTTTAAAG 
GAGCTTTTAA AGTATAGCAA AGAAGGTGAA GGAACTACTA AATTGAAGGA GGCCCTTGAC 
TCAATGCTGG AGCTGCTCAA GTCAGTGAAT GATTCCATGC ATCAGACTGC CATCAATGGC 
TATGTTGGAA ATATGAATGA TCTGGGAAAG ATGATTCTTC AAGGTGCCTT CAGTGTTTGG 
GTAGGACATC GGAAAGGTGC TACCAAGATG AAGGATTTTG CCCGGTTCAA ACCCATGCAG 
AGACACCTTT TCTTGTATGA AAAAGCTGTT ATGTTTTGTA AGAGGCGTTT CGAATCTGGA 
GAAGGTGCTG ACAGATACCC ATCATACAGT TTTAAGCACT GCCTAAAAAT GGAAGAAGCT 
GGTATCACAG AGCATGTGAA AGGCGATAAC CGAAAATTTG AAATATGGTA TGGTGAGAAG 
GAAGAAATTT ATATTGTTCA GGCTCCAAAT ATTGATGTGA AGATGTTATG GCTAAAAGAA 
ATAAGAAACA TTTTGTTGAA GCAGCAGGAA CTTTTGACTG CTAAAACCCA GCAGGATCAG 
TTAAGCGACC AGGACCAACT TCCTCCCCAG CAGCAGAATG CTGAAAAGCA AGAAGAAACT 
CTTTTAAATG CTGTGGAAGC AGGAGCAGAA GTGGAACAAG CGAGTGCCAT GATGGAGGTC 
AGGGAGGCAG CTCTGCTAGT TCAGGCAGAA GCAAATTGCA CAACTTGGAA TGGGATGCTG 
CCATGTACAG AAGGCACCGC TGCCACTGCT GAGCATCCAT GCAACTCTCT CTCCCCTAAC 
AGCGACAATG GAGAAGACAG GTCACAAATG GTTAGTATGA AATCGTTTGT ACATCAGAAT 
CTTCTCCCTA ACGGGAACTG TCTTGCCCCA AAGCACTTGC TGCTCTTACA CAGGACCCAG 
AAGCTACAGT CATTTGGGAA AAGGAATGTT TACTATTCTG TCCCCCATTC TCCTGAAGGA 
GTCCCAGTCC CAGACTTCGA GGCTGTCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756800.2
CDS	381..3833
Translation	MAEARSPPTGPSPAGPPPAGSPPAPALASPWLAFRNLPKAASRAPRTRKRQLNPDNGTFVPFTQTIPVQVDMAMALHRVRIAEKYGKNYLCLLQAVFLAETGLLLQLKDINHFLMQDIAFLSGGRGRDNAWIITFPENCNFRCIPEEVIAKVLNYLTSIARQSGSDSRFTIILDRRMDTWSSLKISLQKISASFPGNLHLVLVLRPTSFLQRTFTDIGFRFSQEDFMLKLPVVMLSSVSDLLTYIDDKQLTPELGGTLQYCHSEWIIFRNVCVSGLTVEPILRRHFCQLNHLLGYRVPHLQILGSWNTNDLTTVTKEGKVLLMNLQVPDTEENLSSSHECPQHINGDWQTIKKLLAQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQEFQEVVTQVEFLLSQQRELGDVTGNLAQVKQRLKKIEILDDKSQELLAKGRIVILHGHKLASNHHYALDLICQRCNELRYLSDILVNEIRSKRIQLSRTFKMHRLLQQARQCCDQGECLLANRGMDKFQTKEDAQKALQDVDNFLQMAMPFINYDIENLQFEFDVLLSPELKAQMKNIQRKLENIRSVFENQQNAYKISKDRPEGAVQRMIPAPESVMRARTIFFSPKHVGIGYSFFQACKIFSKVKKSWRQIQAQSNVKVQATQDSQEKNFDQPPKLDSSLDILKNHVLNELIQTERAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIFMSRLEDCSDAPERVGPCFLERKEDFQMYAKYCQNKPRSELIWRKYSECAFFQECQRKLKHRLGLDSYLLKPVQRITKYQLLLKELLKYSKEGEGTTKLKEALDSMLELLKSVNDSMHQTAINGYVGNMNDLGKMILQGAFSVWVGHRKGATKMKDFARFKPMQRHLFLYEKAVMFCKRRFESGEGADRYPSYSFKHCLKMEEAGITEHVKGDNRKFEIWYGEKEEIYIVQAPNIDVKMLWLKEIRNILLKQQELLTAKTQQDQLSDQDQLPPQQQNAEKQEETLLNAVEAGAEVEQASAMMEVREAALLVQAEANCTTWNGMLPCTEGTAATAEHPCNSLSPNSDNGEDRSQMVSMKSFVHQNLLPNGNCLAPKHLLLLHRTQKLQSFGKRNVYYSVPHSPEGVPVPDFEAVQ

Target ORF information:

RefSeq Version	XM_008758578.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MCF.2 cell line derived transforming sequence (Mcf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758578.2

ORF Insert Sequence:

ATGGCGGAAG CAAGGTCCCC TCCAACAGGG CCCTCTCCAG CAGGGCCCCC TCCAGCAGGA 
TCCCCTCCGG CTCCCGCCCT TGCCTCGCCG TGGCTGGCGT TCAGGAACTT GCCTAAGGCT 
GCCTCCCGGG CGCCCAGGAC TAGGAAACGG CAGCTAAATC CAGACAACGG TACTTTCGTG 
CCTTTCACGC AGACGATCCC GGTGCAGGTG GACATGGCCA TGGCCCTGCA TCGCGTTAGG 
ATCGCAGAGA AGTATGGGAA GAACTACCTC TGCTTGCTGC AGGCAGTCTT CCTCGCAGAA 
ACTGGGCTGC TCCTCCAGTT AAAGGATATC AATCATTTCC TAATGCAAGA CATTGCCTTC 
CTGTCTGGTG GCCGGGGAAG GGACAATGCT TGGATCATTA CGTTTCCAGA AAACTGTAAC 
TTCAGATGCA TACCAGAGGA AGTCATTGCA AAAGTGCTCA ATTACTTGAC ATCTATTGCA 
AGACAAAGTG GATCTGACTC CCGATTTACT ATTATTCTGG ATCGAAGAAT GGATACATGG 
TCTTCCCTCA AAATTTCCCT CCAGAAAATC TCAGCCTCTT TCCCTGGGAA CTTACACTTA 
GTCCTGGTTT TACGTCCTAC CAGTTTTCTT CAACGGACTT TCACAGACAT TGGATTTCGA 
TTTAGCCAGG AGGATTTTAT GCTCAAATTA CCAGTTGTTA TGCTGAGCTC AGTTAGTGAT 
TTGCTGACAT ACATTGATGA CAAGCAGTTA ACCCCTGAGT TAGGCGGCAC CTTGCAGTAC 
TGCCACAGTG AATGGATCAT CTTCAGAAAT GTATGTGTGT CTGGCCTTAC TGTAGAGCCT 
ATCCTTCGTA GGCACTTTTG CCAACTCAAC CACCTCCTTG GCTATAGAGT GCCACACTTG 
CAGATTCTTG GATCCTGGAA TACTAATGAC TTAACTACTG TAACCAAAGA AGGAAAAGTT 
CTTCTCATGA ACCTGCAAGT GCCTGACACT GAAGAAAATC TCAGTTCAAG TCATGAATGT 
CCCCAGCATA TAAATGGTGA CTGGCAAACT ATTAAAAAGT TGCTGGCTCA GGTGCATGAT 
ATGGAAACTG CTTTTGATGG ATTTTGGGAA AAACATCAGC TGAAAATGGA GCAATATCTG 
CAACTGTGGA AGTTTGAGCA AGAATTTCAA GAGGTTGTGA CTCAAGTTGA ATTTTTATTA 
AGTCAACAGA GAGAACTGGG TGATGTAACG GGGAACTTAG CTCAAGTAAA ACAAAGACTT 
AAAAAAATAG AGATCTTGGA TGACAAATCT CAGGAACTGT TAGCCAAGGG CCGGATTGTG 
ATATTGCATG GCCACAAGCT GGCATCGAAT CACCACTATG CCCTTGATTT AATCTGCCAG 
AGGTGTAATG AGCTACGCTA CCTTTCTGAT ATTTTAGTTA ATGAGATCAG ATCAAAGCGG 
ATCCAGCTGA GTAGGACTTT CAAAATGCAC AGACTTCTAC AGCAGGCACG TCAGTGCTGT 
GATCAAGGGG AATGCCTCCT GGCTAACCGG GGAATGGATA AGTTTCAGAC TAAAGAAGAT 
GCTCAGAAAG CCCTTCAGGA TGTGGACAAT TTCCTTCAAA TGGCCATGCC TTTCATCAAT 
TATGATATCG AAAACCTGCA ATTTGAATTT GATGTGCTCC TATCTCCTGA ACTCAAGGCT 
CAAATGAAGA ATATACAACG AAAGCTTGAA AATATTCGAA GTGTATTTGA GAATCAACAG 
AATGCTTATA AGATCTCAAA AGATAGACCT GAGGGGGCAG TCCAGCGTAT GATTCCGGCA 
CCTGAAAGTG TGATGAGAGC CAGAACAATA TTCTTTTCAC CTAAACATGT TGGAATTGGA 
TATTCTTTCT TTCAAGCATG TAAAATTTTT TCAAAAGTGA AAAAGTCTTG GAGACAGATT 
CAAGCTCAGA GCAATGTGAA AGTCCAAGCC ACTCAGGATA GCCAAGAGAA GAATTTTGAT 
CAGCCTCCCA AATTGGACAG TAGCTTGGAT ATTTTAAAGA ACCATGTTCT GAATGAACTG 
ATCCAGACAG AGAGGGCCTA TGTTCGAGAG CTGTTTACTG TTTTGTTGGG CTACCGATCT 
GAGATGGACA ATCCACAGAT GTTTGATCTT ATGCCACCTC TCCTGAGAAA TAAAAAGGAT 
GTTCTCTTTG GAAATATGGC AGAAATATAT GAATTTCATA ACAACATTTT CATGAGCAGA 
CTGGAAGATT GCTCTGATGC TCCAGAAAGA GTGGGACCTT GCTTCCTGGA AAGGAAGGAA 
GATTTTCAGA TGTATGCCAA ATACTGTCAG AATAAACCCA GGTCAGAGTT AATTTGGAGG 
AAGTATTCTG AATGTGCCTT TTTCCAGGAA TGTCAACGGA AATTGAAACA TCGACTTGGT 
CTGGATTCCT ATTTGCTCAA GCCAGTTCAA CGAATCACTA AGTATCAGTT GCTTTTAAAG 
GAGCTTTTAA AGTATAGCAA AGAAGGTGAA GGAACTACTA AATTGAAGGA GGCCCTTGAC 
TCAATGCTGG AGCTGCTCAA GTCAGTGAAT GATTCCATGC ATCAGACTGC CATCAATGGC 
TATGTTGGAA ATATGAATGA TCTGGGAAAG ATGATTCTTC AAGGTGCCTT CAGTGTTTGG 
GTAGGACATC GGAAAGGTGC TACCAAGATG AAGGATTTTG CCCGGTTCAA ACCCATGCAG 
AGACACCTTT TCTTGTATGA AAAAGCTGTT ATGTTTTGTA AGAGGCGTTT CGAATCTGGA 
GAAGGTGCTG ACAGATACCC ATCATACAGT TTTAAGCACT GCCTAAAAAT GGAAGAAGCT 
GGTATCACAG AGCATGTGAA AGGCGATAAC CGAAAATTTG AAATATGGTA TGGTGAGAAG 
GAAGAAATTT ATATTGTTCA GGCTCCAAAT ATTGATGTGA AGATGTTATG GCTAAAAGAA 
ATAAGAAACA TTTTGTTGAA GCAGCAGGAA CTTTTGACTG CTAAAACCCA GCAGGATCAG 
TTAAGCGACC AGGACCAACT TCCTCCCCAG CAGCAGAATG CTGAAAAGCA AGAAGAAACT 
CTTTTAAATG CTGTGGAAGC AGGAGCAGAA GTGGAACAAG CGAGTGCCAT GATGGAGGTC 
AGGGAGGCAG CTCTGCTAGT TCAGGCAGAA GCAAATTGCA CAACTTGGAA TGGGATGCTG 
CCATGTACAG AAGGCACCGC TGCCACTGCT GAGCATCCAT GCAACTCTCT CTCCCCTAAC 
AGCGACAATG GAGAAGACAG GTCACAAATG GTTAGTATGA AATCGTTTGT ACATCAGAAT 
CTTCTCCCTA ACGGGAACTG TCTTGCCCCA AAGCACTTGC TGCTCTTACA CAGGACCCAG 
AAGCTACAGT CATTTGGGAA AAGGAATGTT TACTATTCTG TCCCCCATTC TCCTGAAGGA 
GTCCCAGTCC CAGACTTCGA GGCTGTCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Mcf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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