Ddx21 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx21
Entrez Gene ID	317399
Full Name	DExD-box helicase 21
Synonyms	Ddx21a,Ddx21b
General protein information	Preferred Names DExD-box helicase 21 Names nucleolar RNA helicase 2 DEAD (Asp-Glu-Ala-Asp) box helicase 21 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21a DEAD (Asp-Glu-Ala-Asp) box polypeptide 21b DEAD box protein 21 DEAD-box helicase 21 RH II/Gu gu-alpha nucleolar RNA helicase Gu nucleolar RNA helicase II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037201.1	NP_001032278.1	nucleolar RNA helicase 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ddx21	NP_001039224.1
Bos taurus (cattle)	DDX21	NP_001076996.1
Pan troglodytes (chimpanzee)	DDX21	XP_507825.2
Macaca mulatta (Rhesus monkey)	DDX21	XP_001110939.1
Gallus gallus (chicken)	DDX50	XP_001232052.1
Danio rerio (zebrafish)	ddx21	NP_001120807.1
Homo sapiens (human)	DDX21	NP_004719.2
Canis lupus familiaris (dog)	DDX21	XP_851746.2
Mus musculus (house mouse)	Ddx21	NP_062426.2
Oryza sativa (rice)	Os09g0520700	NP_001063702.1
Rattus norvegicus (Norway rat)	Ddx21	NP_001032278.1
Arabidopsis thaliana (thale cress)	PRH75	NP_201025.1

Gene Ontology
Bibliography

Related articles in PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Treiber T, Treiber N, Plessmann U, Harlander S, Daiß JL, Eichner N, Lehmann G, Schall K, Urlaub H, Meister G
Molecular cell66(2)270-284.e13(2017 Apr)

RNA helicase DDX21 coordinates transcription and ribosomal RNA processing.
Calo E, Flynn RA, Martin L, Spitale RC, Chang HY, Wysocka J
Nature518(7538)249-53(2015 Feb)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Zhang Z, Kim T, Bao M, Facchinetti V, Jung SY, Ghaffari AA, Qin J, Cheng G, Liu YJ
Immunity34(6)866-78(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09200	NM_001037201.1 Latest version!	Rattus norvegicus DExD-box helicase 21 (Ddx21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx21 ( NM_001037201.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001032278.1 Rattus norvegicus DExD-box helicase 21 (Ddx21), mRNA.

Price & Availability↑

CloneID	ORa09200
Clone ID Related Accession (Same CDS sequence)	NM_001037201.1
Accession Version	NM_001037201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	nucleolar RNA helicase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105878.1. On Nov 18, 2005 this sequence version replaced XM_228810.3. ##Evidence-Data-START## Transcript exon combination :: BC105878.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGGAA AACTCCGCAG TGCGTCCAAG TCGGAGTCAG AAGGGACCGA GGAGAGTATG 
GAGACGCTGC AGAAGCCAAG CGAGAAGAAA ACTAGAAAAG AGAAGCCAAA ATCTAAGACT 
GACGAGGCAA CAGAAGGAGT GGAAGAGGCC GCTTCCTCCA AAGTGAAAGC AGTTAAAAAG 
AAAGGGCCTT CTGAGGATGA TGTGGGTCCC CCTAAATCCA AGAAGGCCAA GAAGCAGGAG 
GAGGAGCCTC AAGATGACCC TGCTTCTAAA AGCAAAACCT CCAAGAAGAA AAAGGAGCCC 
CTAGAGAAGA AAGCACCCTC TGCTAAAACC AAAGAAATGA AAGCCGAGGA GCCTTCTGAG 
GAAGAGGCAG ACGCTCCTAA GCCCAAAAAG ACAAAGAAAG GGAAGGAAGC AAACGGAGAT 
GTGGGAGAGA AGAGCCCCGG GCTGAAGAAT GGGCTCTCCC ATCCCAAGCC AGACTCCAGC 
TCCACCCAAG CTCCCGGAGA GGAAAGCGAG ACAGAAAAGG AAATACCCGT GGAGCAGAAA 
GAAGGAGCCT TCTCTAATTT TCCCATATCA GAAGAGACTG TCAAACTTCT CAAAGCTCGT 
GGGGTGAACT TCCTCTTCCC TATACAAGCC AAGACATTCC ACCATGTGTA CAGTGGGAAG 
GACTTAATCG CCCAGGCACG CACAGGAACC GGGAAGACCT TCTCCTTTGC CATCCCTTTG 
ATTGAGAAAC TTCAAGGTGG GCTGCAAGAG AGGAAGAGAG GCCGAGCCCC TCAGGTGCTA 
GTCCTTGCAC CTACAAGAGA ATTAGCAAAT CAAGTGAGCA AAGACTTCAG TGACATCACC 
AAAAAGCTCT CAGTAGCCTG TTTTTATGGT GGAACTCCCT ATGGTGGTCA AATCGAACGA 
ATGCGGAGTG GGATTGACAT CCTGGTCGGG ACCCCAGGTC GTATTAAAGA CCACTTGCAG 
AATGGCAAGC TAGACCTCAC CAAACTTAAA CATGTTGTCC TGGATGAAGT TGATCAGATG 
TTGGATATGG GCTTTGCTGA TCAAGTGGAA GAAATTTTAT GTGTGGCATA CAAGAAAGAT 
TCTGAAGACA ACCCCCAAAC ATTGCTTTTC TCTGCAACTT GCCCTCATTG GGTATTTAAT 
GTTGCTAAGA AATACATGAA ATCTACATAC GAACAGGTGG ACCTGATTGG TAAAAAGACT 
CAGAAAGCAG CCATAACTGT GGAGCACCTG GCGATTAAGT GTCACTGGAC AGAGAGGGCA 
GCGGTGATTG GGGATGTGAT CCGAGTGTAC AGTGGTCACC AAGGGCGTAC AATCATCTTC 
TGCGAAACCA AGAAGGATGC ACAGGAGCTG TCGCAGAACA CGTGCATAAA GCAGGATGCC 
CAGTCCTTAC ATGGCGACAT TCCACAGAAG CAAAGGGAAA TCACCCTGAA AGGTTTCCGA 
AATGGCAATT TTGGAGTTTT GGTGGCAACC AATGTTGCTG CCCGTGGGTT AGACATCCCT 
GAGGTTGACC TGGTTGTTCA GAGCTGTCCA CCAAAGGATG TGGAGTCTTA CATTCATCGT 
TCGGGGCGGA CAGGCAGAGC TGGAAGAACA GGGGTTTGCA TCTGCTTTTA TCAGCACAAA 
GAAGAGTACC AGTTAGCACA AGTGGAACAG AAAGCGGGAA TTAAATTTAA ACGGATAGGT 
GTTCCTTCTG CAACAGAAAT AATAAAAGCC TCCAGCAAAG ACGCAATCAG GCTCTTGGAT 
TCTGTGCCTC CCACTGCTAT TGGCCATTTC AAGCAGTCGG CTGAGAAGCT GATCGAGGAG 
AAGGGAGCTG TAGAGGCCCT GGCCGCAGCT CTGGCCCACA TCTCGGGGGC CACATCGGTG 
GACCAGCGCT CCCTAATCAA CTCACAAGCG GGCTTTGTGA CCATGATCCT GCGGTGTTCT 
GTTGAGATGC CCAACATTAG TTATGCCTGG AAAGAACTTA AGGAGCAGCT GGGTGAGAGC 
ATCGATGCCA AAGTGAAGGG GATGGTCTTC CTCAAAGGAA AACTGGGAGT TTGTTTTGAT 
GTCCGCACTG AAGCAGTCAC AGAAATAAAG GAGAAGTGGC ACGATTCGAG ACGCTGGCAG 
CTCACTGTGG CCACCGAGCA GCCGGAGCTG GAAGGACCCC CAGAAGGATA CCGAGGCGGC 
AGGGGCCAGC GGGATGGCAG CCGTGGCTCT TTCAGAGGAC AGCGGGGTGG AAGCAGGAAC 
TTCCGGGGAC AGGGACAGCG AGGAGGAAGT AGAAACTTCA GAGGACAGCG ACCAGGAGGT 
GGCAACAAAA GTAACAGGTC CCCAAACAAA GGCCAGAAGC GGAGTTTTAG TAAAGCATTT 
GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032278.1
CDS	45..2393
Translation	MPGKLRSASKSESEGTEESMETLQKPSEKKTRKEKPKSKTDEATEGVEEAASSKVKAVKKKGPSEDDVGPPKSKKAKKQEEEPQDDPASKSKTSKKKKEPLEKKAPSAKTKEMKAEEPSEEEADAPKPKKTKKGKEANGDVGEKSPGLKNGLSHPKPDSSSTQAPGEESETEKEIPVEQKEGAFSNFPISEETVKLLKARGVNFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLQGGLQERKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQIERMRSGIDILVGTPGRIKDHLQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILCVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKAAITVEHLAIKCHWTERAAVIGDVIRVYSGHQGRTIIFCETKKDAQELSQNTCIKQDAQSLHGDIPQKQREITLKGFRNGNFGVLVATNVAARGLDIPEVDLVVQSCPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLAQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAIGHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSQAGFVTMILRCSVEMPNISYAWKELKEQLGESIDAKVKGMVFLKGKLGVCFDVRTEAVTEIKEKWHDSRRWQLTVATEQPELEGPPEGYRGGRGQRDGSRGSFRGQRGGSRNFRGQGQRGGSRNFRGQRPGGGNKSNRSPNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	NM_001037201.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DExD-box helicase 21 (Ddx21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037201.1

ORF Insert Sequence:

ATGCCGGGAA AACTCCGCAG TGCGTCCAAG TCGGAGTCAG AAGGGACCGA GGAGAGTATG 
GAGACGCTGC AGAAGCCAAG CGAGAAGAAA ACTAGAAAAG AGAAGCCAAA ATCTAAGACT 
GACGAGGCAA CAGAAGGAGT GGAAGAGGCC GCTTCCTCCA AAGTGAAAGC AGTTAAAAAG 
AAAGGGCCTT CTGAGGATGA TGTGGGTCCC CCTAAATCCA AGAAGGCCAA GAAGCAGGAG 
GAGGAGCCTC AAGATGACCC TGCTTCTAAA AGCAAAACCT CCAAGAAGAA AAAGGAGCCC 
CTAGAGAAGA AAGCACCCTC TGCTAAAACC AAAGAAATGA AAGCCGAGGA GCCTTCTGAG 
GAAGAGGCAG ACGCTCCTAA GCCCAAAAAG ACAAAGAAAG GGAAGGAAGC AAACGGAGAT 
GTGGGAGAGA AGAGCCCCGG GCTGAAGAAT GGGCTCTCCC ATCCCAAGCC AGACTCCAGC 
TCCACCCAAG CTCCCGGAGA GGAAAGCGAG ACAGAAAAGG AAATACCCGT GGAGCAGAAA 
GAAGGAGCCT TCTCTAATTT TCCCATATCA GAAGAGACTG TCAAACTTCT CAAAGCTCGT 
GGGGTGAACT TCCTCTTCCC TATACAAGCC AAGACATTCC ACCATGTGTA CAGTGGGAAG 
GACTTAATCG CCCAGGCACG CACAGGAACC GGGAAGACCT TCTCCTTTGC CATCCCTTTG 
ATTGAGAAAC TTCAAGGTGG GCTGCAAGAG AGGAAGAGAG GCCGAGCCCC TCAGGTGCTA 
GTCCTTGCAC CTACAAGAGA ATTAGCAAAT CAAGTGAGCA AAGACTTCAG TGACATCACC 
AAAAAGCTCT CAGTAGCCTG TTTTTATGGT GGAACTCCCT ATGGTGGTCA AATCGAACGA 
ATGCGGAGTG GGATTGACAT CCTGGTCGGG ACCCCAGGTC GTATTAAAGA CCACTTGCAG 
AATGGCAAGC TAGACCTCAC CAAACTTAAA CATGTTGTCC TGGATGAAGT TGATCAGATG 
TTGGATATGG GCTTTGCTGA TCAAGTGGAA GAAATTTTAT GTGTGGCATA CAAGAAAGAT 
TCTGAAGACA ACCCCCAAAC ATTGCTTTTC TCTGCAACTT GCCCTCATTG GGTATTTAAT 
GTTGCTAAGA AATACATGAA ATCTACATAC GAACAGGTGG ACCTGATTGG TAAAAAGACT 
CAGAAAGCAG CCATAACTGT GGAGCACCTG GCGATTAAGT GTCACTGGAC AGAGAGGGCA 
GCGGTGATTG GGGATGTGAT CCGAGTGTAC AGTGGTCACC AAGGGCGTAC AATCATCTTC 
TGCGAAACCA AGAAGGATGC ACAGGAGCTG TCGCAGAACA CGTGCATAAA GCAGGATGCC 
CAGTCCTTAC ATGGCGACAT TCCACAGAAG CAAAGGGAAA TCACCCTGAA AGGTTTCCGA 
AATGGCAATT TTGGAGTTTT GGTGGCAACC AATGTTGCTG CCCGTGGGTT AGACATCCCT 
GAGGTTGACC TGGTTGTTCA GAGCTGTCCA CCAAAGGATG TGGAGTCTTA CATTCATCGT 
TCGGGGCGGA CAGGCAGAGC TGGAAGAACA GGGGTTTGCA TCTGCTTTTA TCAGCACAAA 
GAAGAGTACC AGTTAGCACA AGTGGAACAG AAAGCGGGAA TTAAATTTAA ACGGATAGGT 
GTTCCTTCTG CAACAGAAAT AATAAAAGCC TCCAGCAAAG ACGCAATCAG GCTCTTGGAT 
TCTGTGCCTC CCACTGCTAT TGGCCATTTC AAGCAGTCGG CTGAGAAGCT GATCGAGGAG 
AAGGGAGCTG TAGAGGCCCT GGCCGCAGCT CTGGCCCACA TCTCGGGGGC CACATCGGTG 
GACCAGCGCT CCCTAATCAA CTCACAAGCG GGCTTTGTGA CCATGATCCT GCGGTGTTCT 
GTTGAGATGC CCAACATTAG TTATGCCTGG AAAGAACTTA AGGAGCAGCT GGGTGAGAGC 
ATCGATGCCA AAGTGAAGGG GATGGTCTTC CTCAAAGGAA AACTGGGAGT TTGTTTTGAT 
GTCCGCACTG AAGCAGTCAC AGAAATAAAG GAGAAGTGGC ACGATTCGAG ACGCTGGCAG 
CTCACTGTGG CCACCGAGCA GCCGGAGCTG GAAGGACCCC CAGAAGGATA CCGAGGCGGC 
AGGGGCCAGC GGGATGGCAG CCGTGGCTCT TTCAGAGGAC AGCGGGGTGG AAGCAGGAAC 
TTCCGGGGAC AGGGACAGCG AGGAGGAAGT AGAAACTTCA GAGGACAGCG ACCAGGAGGT 
GGCAACAAAA GTAACAGGTC CCCAAACAAA GGCCAGAAGC GGAGTTTTAG TAAAGCATTT 
GGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.
Molecular cell66(2)270-284.e13(2017 Apr)
Treiber T,Treiber N,Plessmann U,Harlander S,Daiß JL,Eichner N,Lehmann G,Schall K,Urlaub H,Meister G

RNA helicase DDX21 coordinates transcription and ribosomal RNA processing.
Nature518(7538)249-53(2015 Feb)
Calo E,Flynn RA,Martin L,Spitale RC,Chang HY,Wysocka J

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells.
Immunity34(6)866-78(2011 Jun)
Zhang Z,Kim T,Bao M,Facchinetti V,Jung SY,Ghaffari AA,Qin J,Cheng G,Liu YJ

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Ddx21 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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