Sgo2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sgo2
Entrez Gene ID	316425
Full Name	shugoshin 2
Synonyms	Sgol2
General protein information	Preferred Names shugoshin 2 Names shugoshin 2 shugoshin-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_006244988.3	XP_006245050.1	shugoshin 2 isoform X1
XM_006244989.3	XP_006245051.1	shugoshin 2 isoform X2
NM_001271191.1	NP_001258120.1	shugoshin 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SGOL2	XP_003949987.1
Canis lupus familiaris (dog)	SGOL2	XP_536023.3
Mus musculus (house mouse)	Sgol2	NP_950172.1
Macaca mulatta (Rhesus monkey)	SGOL2	XP_001091712.1
Mus musculus (house mouse)	Gm4975	NP_001182616.1
Rattus norvegicus (Norway rat)	Sgol2	NP_001258120.1
Homo sapiens (human)	SGOL2	NP_689737.4

Gene Ontology
Bibliography

Related articles in PubMed

Meikin is a conserved regulator of meiosis-I-specific kinetochore function.
Kim J, Ishiguro K, Nambu A, Akiyoshi B, Yokobayashi S, Kagami A, Ishiguro T, Pendas AM, Takeda N, Sakakibara Y, Kitajima TS, Tanno Y, Sakuno T, Watanabe Y
Nature517(7535)466-71(2015 Jan)

Sequential assembly of centromeric proteins in male mouse meiosis.
Parra MT, Gómez R, Viera A, Llano E, Pendás AM, Rufas JS, Suja JA
PLoS genetics5(3)e1000417(2009 Mar)

Shugoshin-2 is essential for the completion of meiosis but not for mitotic cell division in mice.
Llano E, Gómez R, Gutiérrez-Caballero C, Herrán Y, Sánchez-Martín M, Vázquez-Quiñones L, Hernández T, de Alava E, Cuadrado A, Barbero JL, Suja JA, Pendás AM
Genes & development22(17)2400-13(2008 Sep)

Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells.
Lee J, Kitajima TS, Tanno Y, Yoshida K, Morita T, Miyano T, Miyake M, Watanabe Y
Nature cell biology10(1)42-52(2008 Jan)

Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.
Huang H, Feng J, Famulski J, Rattner JB, Liu ST, Kao GD, Muschel R, Chan GK, Yen TJ
The Journal of cell biology177(3)413-24(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sgo2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sgo2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10493	NM_001271191.1 Latest version!	Rattus norvegicus shugoshin 2 (Sgo2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa10493	XM_006244988.3 Latest version!	Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa32235	XM_006244989.3 Latest version!	Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sgo2 ( NM_001271191.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258120.1 Rattus norvegicus shugoshin 2 (Sgo2), mRNA.

Price & Availability↑

CloneID	ORa10493
Clone ID Related Accession (Same CDS sequence)	XM_006244988.3 , NM_001271191.1
Accession Version	NM_001271191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Rattus norvegicus(Norway rat)
Product	shugoshin 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067807.1. On or before Sep 7, 2012 this sequence version replaced XM_237176.4, XM_001068385.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGGTAGCAGA ACATCCCTGG TCTTGGTGGA TAACAGTTCT 
GTTTCTAGTA ACACTGCTAA CCCTGAAAAT GAGTCAGAAA GGCACTCTTC ACACCAGATG 
AGGAGGAAAA GACAGTGCAC CCCTCTCAAC CTGACCGAAC CAAGCCTTAG AAGCAAGATG 
AGAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258120.1
CDS	1..3489
Translation	MEYPGIEDDSVSSGIKRRVKDRIAKTKLNVSLASKIKTKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKVQLQKEVEKLNFENTFLRLKLNNLNKKLIEIESQVSNSLLTAIEMSSLSEFHQSSFLLSANKKKRVSKQCKPAHLPYARVLITSENDDDDDDGDDDKWKTKCNNRTVSKTSPDSTSSVSRQSLSLHQCNLEVFLPQEDNQKTCESGHSEYTSSVDVLPNESHYHSDQSPKSFLSKMKDAPSPSPIKEKLSLGNVTKRKKCVSSAADILHVADLDHQSISSPGSNWNNEINGCTNETSNNVQRNTECFPDLPSESSSGPNVKCMEQVQKNTDTFHLQKTVYEDADMELTATDIGKIVTISKSNKSQNKKKADCRKETFRKVKGPSSDRKKERSKRKCKASSEGAEGKTENRPERGSGVLDGRGDSEDPNCISNSEQPSQVNMQKEITFQKSSDQENVQNQRRRQTYTTDEQEETNPFSRCSVKFLQDGKFDPYQNTLHCDLSKPSRQTFVIQTSEKDNLLPNQDNKENISENLEDANEFHIAHLSIKDNENVCDHETQTILDLKKNVNAQQNQTKINKTKQKINRRTKIISITNQINEDNDKDIHVLEKGNFPFQTQANKETTSGNLEASKEFETPLYTRDSGSLHDCNTQNVLDLHKQITSIYPGQNKSQISKIPRQKLSRKTEVISEVNCFSNDRDVLCSEKDESFLLQKDKEIPGTLKDVSEFDKPAFYNKDSVKSCDFKAEPLLGLKRHDPNMPPACQADSKVDKKLRQKVKRKTEIISKINQIHKNDEGSAHDPLNKELRQKVSISKIISEMNQIYETINEDGNAFKSSLKHCQDVMSCDFGKKTSNEEENHDLTPDLCSLVKKPRKKVSCKAGNSLTGDKNRCILQLTGSSQESVTLDSGLKHGPNEADSGPGEQTNLPKKQKPSTVSTLGDAFSLSVVREGSHPAQTGSKVTSKSKKRKTPLRSSDTRGAVERTPNTDGITAVYFQQTEKENYLENEKIAKSKPDFRSKVLKPLSEICSSNIKNSSLGSMCESSVPLSTFSSKPLMLEESSSLDSTCIFPVGDGAHEKLKEGTPTSHRRTQKSRPGSRTSLVLVDNSSVSSNTANPENESERHSSHQMRRKRQCTPLNLTEPSLRSKMRR

Target ORF information:

RefSeq Version	NM_001271191.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus shugoshin 2 (Sgo2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271191.1

ORF Insert Sequence:

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGGTAGCAGA ACATCCCTGG TCTTGGTGGA TAACAGTTCT 
GTTTCTAGTA ACACTGCTAA CCCTGAAAAT GAGTCAGAAA GGCACTCTTC ACACCAGATG 
AGGAGGAAAA GACAGTGCAC CCCTCTCAAC CTGACCGAAC CAAGCCTTAG AAGCAAGATG 
AGAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgo2 ( XM_006244988.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245050.1 Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10493
Clone ID Related Accession (Same CDS sequence)	XM_006244988.3 , NM_001271191.1
Accession Version	XM_006244988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	shugoshin 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGGTAGCAGA ACATCCCTGG TCTTGGTGGA TAACAGTTCT 
GTTTCTAGTA ACACTGCTAA CCCTGAAAAT GAGTCAGAAA GGCACTCTTC ACACCAGATG 
AGGAGGAAAA GACAGTGCAC CCCTCTCAAC CTGACCGAAC CAAGCCTTAG AAGCAAGATG 
AGAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245050.1
CDS	319..3807
Translation	MEYPGIEDDSVSSGIKRRVKDRIAKTKLNVSLASKIKTKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKVQLQKEVEKLNFENTFLRLKLNNLNKKLIEIESQVSNSLLTAIEMSSLSEFHQSSFLLSANKKKRVSKQCKPAHLPYARVLITSENDDDDDDGDDDKWKTKCNNRTVSKTSPDSTSSVSRQSLSLHQCNLEVFLPQEDNQKTCESGHSEYTSSVDVLPNESHYHSDQSPKSFLSKMKDAPSPSPIKEKLSLGNVTKRKKCVSSAADILHVADLDHQSISSPGSNWNNEINGCTNETSNNVQRNTECFPDLPSESSSGPNVKCMEQVQKNTDTFHLQKTVYEDADMELTATDIGKIVTISKSNKSQNKKKADCRKETFRKVKGPSSDRKKERSKRKCKASSEGAEGKTENRPERGSGVLDGRGDSEDPNCISNSEQPSQVNMQKEITFQKSSDQENVQNQRRRQTYTTDEQEETNPFSRCSVKFLQDGKFDPYQNTLHCDLSKPSRQTFVIQTSEKDNLLPNQDNKENISENLEDANEFHIAHLSIKDNENVCDHETQTILDLKKNVNAQQNQTKINKTKQKINRRTKIISITNQINEDNDKDIHVLEKGNFPFQTQANKETTSGNLEASKEFETPLYTRDSGSLHDCNTQNVLDLHKQITSIYPGQNKSQISKIPRQKLSRKTEVISEVNCFSNDRDVLCSEKDESFLLQKDKEIPGTLKDVSEFDKPAFYNKDSVKSCDFKAEPLLGLKRHDPNMPPACQADSKVDKKLRQKVKRKTEIISKINQIHKNDEGSAHDPLNKELRQKVSISKIISEMNQIYETINEDGNAFKSSLKHCQDVMSCDFGKKTSNEEENHDLTPDLCSLVKKPRKKVSCKAGNSLTGDKNRCILQLTGSSQESVTLDSGLKHGPNEADSGPGEQTNLPKKQKPSTVSTLGDAFSLSVVREGSHPAQTGSKVTSKSKKRKTPLRSSDTRGAVERTPNTDGITAVYFQQTEKENYLENEKIAKSKPDFRSKVLKPLSEICSSNIKNSSLGSMCESSVPLSTFSSKPLMLEESSSLDSTCIFPVGDGAHEKLKEGTPTSHRRTQKSRPGSRTSLVLVDNSSVSSNTANPENESERHSSHQMRRKRQCTPLNLTEPSLRSKMRR

Target ORF information:

RefSeq Version	XM_006244988.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244988.3

ORF Insert Sequence:

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGGTAGCAGA ACATCCCTGG TCTTGGTGGA TAACAGTTCT 
GTTTCTAGTA ACACTGCTAA CCCTGAAAAT GAGTCAGAAA GGCACTCTTC ACACCAGATG 
AGGAGGAAAA GACAGTGCAC CCCTCTCAAC CTGACCGAAC CAAGCCTTAG AAGCAAGATG 
AGAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sgo2 ( XM_006244989.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245051.1 Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X2, mRNA.

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CloneID	ORa32235
Clone ID Related Accession (Same CDS sequence)	XM_006244989.3
Accession Version	XM_006244989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	shugoshin 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245051.1
CDS	319..3648
Translation	MEYPGIEDDSVSSGIKRRVKDRIAKTKLNVSLASKIKTKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKVQLQKEVEKLNFENTFLRLKLNNLNKKLIEIESQVSNSLLTAIEMSSLSEFHQSSFLLSANKKKRVSKQCKPAHLPYARVLITSENDDDDDDGDDDKWKTKCNNRTVSKTSPDSTSSVSRQSLSLHQCNLEVFLPQEDNQKTCESGHSEYTSSVDVLPNESHYHSDQSPKSFLSKMKDAPSPSPIKEKLSLGNVTKRKKCVSSAADILHVADLDHQSISSPGSNWNNEINGCTNETSNNVQRNTECFPDLPSESSSGPNVKCMEQVQKNTDTFHLQKTVYEDADMELTATDIGKIVTISKSNKSQNKKKADCRKETFRKVKGPSSDRKKERSKRKCKASSEGAEGKTENRPERGSGVLDGRGDSEDPNCISNSEQPSQVNMQKEITFQKSSDQENVQNQRRRQTYTTDEQEETNPFSRCSVKFLQDGKFDPYQNTLHCDLSKPSRQTFVIQTSEKDNLLPNQDNKENISENLEDANEFHIAHLSIKDNENVCDHETQTILDLKKNVNAQQNQTKINKTKQKINRRTKIISITNQINEDNDKDIHVLEKGNFPFQTQANKETTSGNLEASKEFETPLYTRDSGSLHDCNTQNVLDLHKQITSIYPGQNKSQISKIPRQKLSRKTEVISEVNCFSNDRDVLCSEKDESFLLQKDKEIPGTLKDVSEFDKPAFYNKDSVKSCDFKAEPLLGLKRHDPNMPPACQADSKVDKKLRQKVKRKTEIISKINQIHKNDEGSAHDPLNKELRQKVSISKIISEMNQIYETINEDGNAFKSSLKHCQDVMSCDFGKKTSNEEENHDLTPDLCSLVKKPRKKVSCKAGNSLTGDKNRCILQLTGSSQESVTLDSGLKHGPNEADSGPGEQTNLPKKQKPSTVSTLGDAFSLSVVREGSHPAQTGSKVTSKSKKRKTPLRSSDTRGAVERTPNTDGITAVYFQQTEKENYLENEKIAKSKPDFRSKVLKPLSEICSSNIKNSSLGSMCESSVPLSTFSSKPLMLEESSSLDSTCIFPVGDGAHEKLKEGTPTSHRRTQKSRPAR

Target ORF information:

RefSeq Version	XM_006244989.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus shugoshin 2 (Sgo2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244989.3

ORF Insert Sequence:

ATGGAGTACC CAGGGATAGA AGATGACTCT GTTTCCTCTG GAATTAAGAG ACGAGTGAAG 
GATAGAATTG CAAAGACAAA GTTGAATGTT TCTCTTGCTT CAAAGATCAA AACAAAAATA 
TTAAACAATT CTTCTATTTT CAAGATCTCT CTAAAGCACA ACAACAGAGC ATTAGCTCGG 
GCCCTTAGTA AAGAGAAAGA GAATTCTCGA AGAATCACTA CCGAAAAGGT GCAATTACAG 
AAAGAAGTAG AGAAACTGAA TTTTGAGAAT ACCTTTCTTC GCTTAAAACT AAATAATCTG 
AATAAGAAGC TGATAGAAAT AGAATCACAA GTGAGCAATA GTTTGTTAAC TGCAATTGAA 
ATGAGCAGTC TTTCTGAGTT CCATCAAAGT TCTTTTCTCC TGTCAGCTAA CAAGAAAAAA 
AGGGTCAGTA AACAATGCAA GCCTGCACAC CTTCCATATG CAAGAGTTCT AATAACTTCA 
GAAAATGATG ATGATGATGA TGATGGTGAT GATGATAAAT GGAAAACAAA ATGTAACAAC 
AGAACTGTAT CAAAGACATC ACCTGATAGT ACCTCTTCTG TATCAAGACA GTCTTTATCC 
TTACATCAGT GTAATTTGGA AGTATTCCTT CCTCAAGAAG ATAATCAGAA AACATGTGAA 
TCAGGTCATT CAGAATATAC TTCAAGTGTT GATGTACTTC CTAATGAGAG CCATTACCAC 
TCAGATCAAA GTCCTAAGAG TTTTCTGAGT AAGATGAAAG ATGCTCCGTC TCCCAGCCCC 
ATAAAGGAAA AATTGTCACT TGGTAATGTG ACTAAGAGGA AGAAGTGTGT GTCTTCAGCT 
GCAGACATTC TGCATGTGGC AGATTTAGAT CACCAATCAA TTTCAAGTCC AGGATCAAAT 
TGGAATAATG AGATAAATGG TTGTACTAAT GAAACCAGTA ATAACGTGCA AAGAAATACA 
GAGTGTTTTC CTGACTTACC TTCTGAGTCT TCCAGTGGTC CTAATGTAAA GTGCATGGAA 
CAAGTACAGA AGAACACTGA TACCTTTCAC TTGCAGAAAA CTGTGTATGA AGACGCTGAC 
ATGGAGTTAA CTGCTACTGA CATAGGCAAG ATTGTAACAA TTTCAAAAAG CAATAAAAGT 
CAAAATAAGA AAAAGGCAGA TTGTAGAAAG GAGACTTTCA GAAAAGTGAA AGGTCCAAGC 
TCTGATAGGA AGAAAGAAAG ATCAAAGAGA AAATGTAAAG CTAGTTCGGA AGGTGCTGAG 
GGAAAGACTG AAAACAGACC AGAGAGAGGC TCTGGTGTCC TAGATGGCAG AGGGGATTCA 
GAAGATCCAA ACTGTATTTC CAATAGTGAA CAGCCATCTC AGGTAAACAT GCAGAAGGAA 
ATAACCTTCC AGAAGAGCTC AGATCAGGAG AACGTTCAAA ATCAAAGGAG GAGGCAAACG 
TATACGACAG ATGAGCAGGA GGAAACAAAC CCTTTCTCCA GATGTTCAGT CAAATTCCTT 
CAAGATGGTA AATTTGATCC GTATCAGAAC ACCCTACACT GTGATTTAAG TAAGCCTTCT 
CGGCAGACAT TTGTGATTCA AACATCAGAA AAAGATAACT TACTGCCAAA CCAAGACAAT 
AAAGAGAACA TTTCTGAAAA CCTAGAAGAT GCAAATGAAT TTCATATAGC TCATCTTTCC 
ATCAAAGATA ATGAAAATGT ATGTGACCAT GAAACTCAAA CAATTTTGGA CTTGAAAAAG 
AATGTCAATG CTCAGCAAAA TCAAACAAAA ATAAATAAGA CTAAGCAGAA AATAAATCGA 
AGAACCAAAA TAATTTCTAT TACAAACCAA ATAAATGAGG ATAATGATAA AGATATTCAC 
GTCCTAGAAA AAGGCAACTT TCCCTTCCAA ACCCAAGCAA ATAAAGAAAC CACCAGTGGA 
AACCTAGAAG CTTCAAAAGA ATTTGAAACA CCTCTTTACA CAAGAGACAG TGGAAGCTTA 
CATGACTGTA ACACCCAGAA TGTTCTGGAT CTGCACAAGC AAATCACTAG TATATACCCT 
GGTCAGAATA AGTCCCAAAT TAGCAAAATC CCTAGACAGA AGCTAAGTCG CAAGACAGAA 
GTAATTTCTG AAGTGAATTG TTTTAGTAAT GACAGAGATG TCCTTTGTTC AGAAAAGGAT 
GAGTCTTTCT TGCTACAAAA GGATAAAGAG ATCCCTGGAA CCTTGAAAGA CGTAAGCGAG 
TTTGATAAGC CTGCTTTTTA TAACAAAGAT AGTGTGAAGT CATGTGATTT TAAGGCTGAA 
CCGTTGTTGG GGTTGAAAAG ACATGACCCT AATATGCCAC CTGCTTGTCA AGCTGACTCA 
AAGGTAGATA AGAAGCTTAG ACAAAAGGTG AAGCGAAAGA CAGAAATAAT TTCTAAAATC 
AACCAGATAC ATAAAAATGA TGAAGGAAGT GCACATGACC CATTAAATAA GGAGCTCCGT 
CAGAAGGTTA GTATATCAAA AATCATTTCT GAAATGAACC AAATATATGA GACTATTAAT 
GAAGATGGGA ATGCCTTTAA AAGCTCGCTC AAACATTGCC AAGATGTTAT GAGTTGTGAC 
TTTGGGAAAA AAACCAGTAA CGAAGAGGAA AATCATGATC TGACTCCAGA TCTTTGCTCA 
CTAGTTAAGA AACCAAGGAA AAAGGTATCA TGTAAAGCAG GGAACAGTTT GACAGGAGAT 
AAGAACAGGT GTATTTTGCA GTTAACAGGT TCTTCACAGG AGTCCGTCAC CTTGGACTCT 
GGCTTAAAAC ACGGTCCAAA TGAAGCAGAT TCTGGCCCTG GAGAGCAGAC TAACCTGCCA 
AAGAAGCAGA AACCAAGCAC TGTGAGTACA CTGGGAGACG CCTTCTCTCT GAGTGTGGTA 
AGAGAAGGAA GCCACCCAGC CCAGACAGGT AGTAAAGTGA CATCCAAGTC AAAGAAAAGA 
AAGACCCCTC TAAGATCCTC TGATACCCGT GGAGCAGTGG AGAGAACACC AAACACTGAT 
GGCATTACGG CAGTTTACTT CCAACAGACT GAGAAGGAGA ACTATTTGGA GAATGAGAAA 
ATTGCCAAGA GTAAGCCAGA CTTTCGCTCA AAGGTGTTGA AACCTTTATC TGAGATATGT 
TCATCTAACA TAAAGAATTC TTCCTTGGGC AGTATGTGTG AGAGTTCTGT GCCTTTGAGT 
ACTTTTTCTA GTAAACCCCT GATGCTAGAA GAAAGTTCTT CCCTGGACAG TACATGCATC 
TTTCCAGTAG GTGATGGTGC CCATGAAAAG TTAAAGGAAG GCACTCCTAC ATCCCACCGC 
AGGACACAGA AGTCAAGACC AGCAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Meikin is a conserved regulator of meiosis-I-specific kinetochore function.
Nature517(7535)466-71(2015 Jan)
Kim J,Ishiguro K,Nambu A,Akiyoshi B,Yokobayashi S,Kagami A,Ishiguro T,Pendas AM,Takeda N,Sakakibara Y,Kitajima TS,Tanno Y,Sakuno T,Watanabe Y

Sequential assembly of centromeric proteins in male mouse meiosis.
PLoS genetics5(3)e1000417(2009 Mar)
Parra MT,Gómez R,Viera A,Llano E,Pendás AM,Rufas JS,Suja JA

Shugoshin-2 is essential for the completion of meiosis but not for mitotic cell division in mice.
Genes & development22(17)2400-13(2008 Sep)
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Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells.
Nature cell biology10(1)42-52(2008 Jan)
Lee J,Kitajima TS,Tanno Y,Yoshida K,Morita T,Miyano T,Miyake M,Watanabe Y

Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.
The Journal of cell biology177(3)413-24(2007 May)
Huang H,Feng J,Famulski J,Rattner JB,Liu ST,Kao GD,Muschel R,Chan GK,Yen TJ

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