Mlc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mlc1
Entrez Gene ID	315215
Full Name	megalencephalic leukoencephalopathy with subcortical cysts 1
General protein information	Preferred Names megalencephalic leukoencephalopathy with subcortical cysts 1 Names membrane protein MLC1 megalencephalic leukoencephalopathy with subcortical cysts 1 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594878.1	XP_017450367.1	membrane protein MLC1 isoform X2
NM_001108105.1	NP_001101575.1	membrane protein MLC1
XM_006242201.3	XP_006242263.1	membrane protein MLC1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	MLC1	XP_001111622.1
Mus musculus (house mouse)	Mlc1	NP_573504.1
Rattus norvegicus (Norway rat)	Mlc1	NP_001101575.1
Danio rerio (zebrafish)	mlc1	NP_001098409.1
Homo sapiens (human)	MLC1	NP_631941.1
Gallus gallus (chicken)	MLC1	XP_004937559.1
Xenopus tropicalis (tropical clawed frog)	mlc1	NP_001072921.1
Canis lupus familiaris (dog)	MLC1	XP_848790.2
Pan troglodytes (chimpanzee)	MLC1	XP_515212.3
Bos taurus (cattle)	MLC1	NP_001070420.1

Gene Ontology
Bibliography

Related articles in PubMed

GlialCAM/MLC1 modulates LRRC8/VRAC currents in an indirect manner: Implications for megalencephalic leukoencephalopathy.
Elorza-Vidal X, Sirisi S, Gaitán-Peñas H, Pérez-Rius C, Alonso-Gardón M, Armand-Ugón M, Lanciotti A, Brignone MS, Prat E, Nunes V, Ambrosini E, Gasull X, Estévez R
Neurobiology of disease11988-99(2018 11)

Megalencephalic leukoencephalopathy with subcortical cysts protein-1 regulates epidermal growth factor receptor signaling in astrocytes.
Lanciotti A, Brignone MS, Visentin S, De Nuccio C, Catacuzzeno L, Mallozzi C, Petrini S, Caramia M, Veroni C, Minnone G, Bernardo A, Franciolini F, Pessia M, Bertini E, Petrucci TC, Ambrosini E
Human molecular genetics25(8)1543-58(2016 Apr)

Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations.
Lanciotti A, Brignone MS, Molinari P, Visentin S, De Nuccio C, Macchia G, Aiello C, Bertini E, Aloisi F, Petrucci TC, Ambrosini E
Human molecular genetics21(10)2166-80(2012 May)

MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation.
Lanciotti A, Brignone MS, Camerini S, Serafini B, Macchia G, Raggi C, Molinari P, Crescenzi M, Musumeci M, Sargiacomo M, Aloisi F, Petrucci TC, Ambrosini E
Neurobiology of disease37(3)581-95(2010 Mar)

Biochemical characterization of MLC1 protein in astrocytes and its association with the dystrophin-glycoprotein complex.
Ambrosini E, Serafini B, Lanciotti A, Tosini F, Scialpi F, Psaila R, Raggi C, Di Girolamo F, Petrucci TC, Aloisi F
Molecular and cellular neurosciences37(3)480-93(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00257	NM_001108105.1 Latest version!	Rattus norvegicus modulator of VRAC current 1 (Mlc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa00257	XM_006242201.3 Latest version!	Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa62021	XM_017594878.1 Latest version!	Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mlc1 ( NM_001108105.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101575.1 Rattus norvegicus modulator of VRAC current 1 (Mlc1), mRNA.

Price & Availability↑

CloneID	ORa00257
Clone ID Related Accession (Same CDS sequence)	NM_001108105.1 , XM_006242201.3
Accession Version	NM_001108105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane protein MLC1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474027.1. On or before Oct 4, 2007 this sequence version replaced XM_235558.4, XM_001054359.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAATACTT CTTGCTGGAA GTCCATCACC CAGAGGCCTG CTCTCCCAAC GGCTGGACCA 
CAGCAGAGTG CCATGACACG GGAGGGGCAG TTCAGGGAGG AGCTGGGCTA TGACCGGATG 
CCCACACTGG AGAGGGGTCG ACAGGATGCA GGACGGCAGG ACACAGGCAG TTACACCCCT 
GACACAAAGC CCAAGGACCT GCAGCTGACG AAGAGGTTGC CTCCGTGCTT CAGCTACAAG 
ACATGGGTAT TCTCTGTGTT GATGGGGAGC TGCCTTCTCG TGACCTCTGG GTTTTCGCTC 
TATCTGGGGA ACGTGTTCCC CTCCGAGATG GATTATTTGC GCTGTGCAGC AGGCTCTTGC 
ATCCCCTCAG CAATCGTGAG CTTCGCTGTT GGGAGGAGAA ATGTCAGTGC GATTCCCAAC 
TTTCAGATAT TGTTCGTTTC CACGTTCGCT GTTACCACTA CGTGCTTAAT CTGGTTTGGA 
TGCAAATTGA TCCTGAATCC TTCAGCCATA AATATTAATT TCAATCTCAT CCTGCTCTTG 
CTGTTGGAGC TCCTCATGGC TGCCACGGTG ATCATCTCGG CCCGGTCCAG TGAGGAGCCC 
TGCAAGAAGA AGAAGGGTTC CATCTCGGAT GGCACTAACA TTCTGGATGA AGTGACTTTT 
CCTGCTCGGG TCCTAAAGTC CTATTCTGTG GTAGAAGTAA TTGCTGGTGT CTCGGCTGTC 
CTCGGTGGGG TGATCGCTCT AAATGTAGAG GAAGCTGTCT CAGGTCCCCA TCTCTCGGTG 
ACGTTCTTTT GGATTTTAGT GGCTTGTTTT CCAAGTGCCA TCGCCAGCCA TGTGACAGCG 
GAATGTCCCA GCAAATGTCT GGTGGAGGTC CTGATCACCA TCAGCAGCCT TACGTCCCCA 
CTGCTGTTCA CCGCCTCTGG ATACCTGTCC TTCAGCGTGA TGAGAATCGT GGAGATTTTT 
AAAGATTACC CACCAGACAT CAAATCCTAT GATGTGCTGC TGCTGCTCCT GCTGCTGCTG 
CTGCTGCTTC AGGGGGGACT CAACACTGGC ACCGCCATCC AGTGTGTGAG CTTCAAAGTC 
AGCGCCCGGC TGCAGGCGGC CTCCTGGGAC TCCCAGTCCT GTCCCCAGGA GCGGCCAGCA 
GGCGAGGTGG TCCGAAGCCC CCTCAAGGAG TTTGACAAAG AAAAAGCCTG GAGAGCTGTG 
GTGGTACAAA TGGCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101575.1
CDS	429..1649
Translation	MNTSCWKSITQRPALPTAGPQQSAMTREGQFREELGYDRMPTLERGRQDAGRQDTGSYTPDTKPKDLQLTKRLPPCFSYKTWVFSVLMGSCLLVTSGFSLYLGNVFPSEMDYLRCAAGSCIPSAIVSFAVGRRNVSAIPNFQILFVSTFAVTTTCLIWFGCKLILNPSAININFNLILLLLLELLMAATVIISARSSEEPCKKKKGSISDGTNILDEVTFPARVLKSYSVVEVIAGVSAVLGGVIALNVEEAVSGPHLSVTFFWILVACFPSAIASHVTAECPSKCLVEVLITISSLTSPLLFTASGYLSFSVMRIVEIFKDYPPDIKSYDVLLLLLLLLLLLQGGLNTGTAIQCVSFKVSARLQAASWDSQSCPQERPAGEVVRSPLKEFDKEKAWRAVVVQMAQ

Target ORF information:

RefSeq Version	NM_001108105.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus modulator of VRAC current 1 (Mlc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108105.1

ORF Insert Sequence:

ATGAATACTT CTTGCTGGAA GTCCATCACC CAGAGGCCTG CTCTCCCAAC GGCTGGACCA 
CAGCAGAGTG CCATGACACG GGAGGGGCAG TTCAGGGAGG AGCTGGGCTA TGACCGGATG 
CCCACACTGG AGAGGGGTCG ACAGGATGCA GGACGGCAGG ACACAGGCAG TTACACCCCT 
GACACAAAGC CCAAGGACCT GCAGCTGACG AAGAGGTTGC CTCCGTGCTT CAGCTACAAG 
ACATGGGTAT TCTCTGTGTT GATGGGGAGC TGCCTTCTCG TGACCTCTGG GTTTTCGCTC 
TATCTGGGGA ACGTGTTCCC CTCCGAGATG GATTATTTGC GCTGTGCAGC AGGCTCTTGC 
ATCCCCTCAG CAATCGTGAG CTTCGCTGTT GGGAGGAGAA ATGTCAGTGC GATTCCCAAC 
TTTCAGATAT TGTTCGTTTC CACGTTCGCT GTTACCACTA CGTGCTTAAT CTGGTTTGGA 
TGCAAATTGA TCCTGAATCC TTCAGCCATA AATATTAATT TCAATCTCAT CCTGCTCTTG 
CTGTTGGAGC TCCTCATGGC TGCCACGGTG ATCATCTCGG CCCGGTCCAG TGAGGAGCCC 
TGCAAGAAGA AGAAGGGTTC CATCTCGGAT GGCACTAACA TTCTGGATGA AGTGACTTTT 
CCTGCTCGGG TCCTAAAGTC CTATTCTGTG GTAGAAGTAA TTGCTGGTGT CTCGGCTGTC 
CTCGGTGGGG TGATCGCTCT AAATGTAGAG GAAGCTGTCT CAGGTCCCCA TCTCTCGGTG 
ACGTTCTTTT GGATTTTAGT GGCTTGTTTT CCAAGTGCCA TCGCCAGCCA TGTGACAGCG 
GAATGTCCCA GCAAATGTCT GGTGGAGGTC CTGATCACCA TCAGCAGCCT TACGTCCCCA 
CTGCTGTTCA CCGCCTCTGG ATACCTGTCC TTCAGCGTGA TGAGAATCGT GGAGATTTTT 
AAAGATTACC CACCAGACAT CAAATCCTAT GATGTGCTGC TGCTGCTCCT GCTGCTGCTG 
CTGCTGCTTC AGGGGGGACT CAACACTGGC ACCGCCATCC AGTGTGTGAG CTTCAAAGTC 
AGCGCCCGGC TGCAGGCGGC CTCCTGGGAC TCCCAGTCCT GTCCCCAGGA GCGGCCAGCA 
GGCGAGGTGG TCCGAAGCCC CCTCAAGGAG TTTGACAAAG AAAAAGCCTG GAGAGCTGTG 
GTGGTACAAA TGGCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlc1 ( XM_006242201.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242263.1 Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00257
Clone ID Related Accession (Same CDS sequence)	NM_001108105.1 , XM_006242201.3
Accession Version	XM_006242201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane protein MLC1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACTT CTTGCTGGAA GTCCATCACC CAGAGGCCTG CTCTCCCAAC GGCTGGACCA 
CAGCAGAGTG CCATGACACG GGAGGGGCAG TTCAGGGAGG AGCTGGGCTA TGACCGGATG 
CCCACACTGG AGAGGGGTCG ACAGGATGCA GGACGGCAGG ACACAGGCAG TTACACCCCT 
GACACAAAGC CCAAGGACCT GCAGCTGACG AAGAGGTTGC CTCCGTGCTT CAGCTACAAG 
ACATGGGTAT TCTCTGTGTT GATGGGGAGC TGCCTTCTCG TGACCTCTGG GTTTTCGCTC 
TATCTGGGGA ACGTGTTCCC CTCCGAGATG GATTATTTGC GCTGTGCAGC AGGCTCTTGC 
ATCCCCTCAG CAATCGTGAG CTTCGCTGTT GGGAGGAGAA ATGTCAGTGC GATTCCCAAC 
TTTCAGATAT TGTTCGTTTC CACGTTCGCT GTTACCACTA CGTGCTTAAT CTGGTTTGGA 
TGCAAATTGA TCCTGAATCC TTCAGCCATA AATATTAATT TCAATCTCAT CCTGCTCTTG 
CTGTTGGAGC TCCTCATGGC TGCCACGGTG ATCATCTCGG CCCGGTCCAG TGAGGAGCCC 
TGCAAGAAGA AGAAGGGTTC CATCTCGGAT GGCACTAACA TTCTGGATGA AGTGACTTTT 
CCTGCTCGGG TCCTAAAGTC CTATTCTGTG GTAGAAGTAA TTGCTGGTGT CTCGGCTGTC 
CTCGGTGGGG TGATCGCTCT AAATGTAGAG GAAGCTGTCT CAGGTCCCCA TCTCTCGGTG 
ACGTTCTTTT GGATTTTAGT GGCTTGTTTT CCAAGTGCCA TCGCCAGCCA TGTGACAGCG 
GAATGTCCCA GCAAATGTCT GGTGGAGGTC CTGATCACCA TCAGCAGCCT TACGTCCCCA 
CTGCTGTTCA CCGCCTCTGG ATACCTGTCC TTCAGCGTGA TGAGAATCGT GGAGATTTTT 
AAAGATTACC CACCAGACAT CAAATCCTAT GATGTGCTGC TGCTGCTCCT GCTGCTGCTG 
CTGCTGCTTC AGGGGGGACT CAACACTGGC ACCGCCATCC AGTGTGTGAG CTTCAAAGTC 
AGCGCCCGGC TGCAGGCGGC CTCCTGGGAC TCCCAGTCCT GTCCCCAGGA GCGGCCAGCA 
GGCGAGGTGG TCCGAAGCCC CCTCAAGGAG TTTGACAAAG AAAAAGCCTG GAGAGCTGTG 
GTGGTACAAA TGGCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242263.1
CDS	318..1538
Translation	MNTSCWKSITQRPALPTAGPQQSAMTREGQFREELGYDRMPTLERGRQDAGRQDTGSYTPDTKPKDLQLTKRLPPCFSYKTWVFSVLMGSCLLVTSGFSLYLGNVFPSEMDYLRCAAGSCIPSAIVSFAVGRRNVSAIPNFQILFVSTFAVTTTCLIWFGCKLILNPSAININFNLILLLLLELLMAATVIISARSSEEPCKKKKGSISDGTNILDEVTFPARVLKSYSVVEVIAGVSAVLGGVIALNVEEAVSGPHLSVTFFWILVACFPSAIASHVTAECPSKCLVEVLITISSLTSPLLFTASGYLSFSVMRIVEIFKDYPPDIKSYDVLLLLLLLLLLLQGGLNTGTAIQCVSFKVSARLQAASWDSQSCPQERPAGEVVRSPLKEFDKEKAWRAVVVQMAQ

Target ORF information:

RefSeq Version	XM_006242201.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242201.3

ORF Insert Sequence:

ATGAATACTT CTTGCTGGAA GTCCATCACC CAGAGGCCTG CTCTCCCAAC GGCTGGACCA 
CAGCAGAGTG CCATGACACG GGAGGGGCAG TTCAGGGAGG AGCTGGGCTA TGACCGGATG 
CCCACACTGG AGAGGGGTCG ACAGGATGCA GGACGGCAGG ACACAGGCAG TTACACCCCT 
GACACAAAGC CCAAGGACCT GCAGCTGACG AAGAGGTTGC CTCCGTGCTT CAGCTACAAG 
ACATGGGTAT TCTCTGTGTT GATGGGGAGC TGCCTTCTCG TGACCTCTGG GTTTTCGCTC 
TATCTGGGGA ACGTGTTCCC CTCCGAGATG GATTATTTGC GCTGTGCAGC AGGCTCTTGC 
ATCCCCTCAG CAATCGTGAG CTTCGCTGTT GGGAGGAGAA ATGTCAGTGC GATTCCCAAC 
TTTCAGATAT TGTTCGTTTC CACGTTCGCT GTTACCACTA CGTGCTTAAT CTGGTTTGGA 
TGCAAATTGA TCCTGAATCC TTCAGCCATA AATATTAATT TCAATCTCAT CCTGCTCTTG 
CTGTTGGAGC TCCTCATGGC TGCCACGGTG ATCATCTCGG CCCGGTCCAG TGAGGAGCCC 
TGCAAGAAGA AGAAGGGTTC CATCTCGGAT GGCACTAACA TTCTGGATGA AGTGACTTTT 
CCTGCTCGGG TCCTAAAGTC CTATTCTGTG GTAGAAGTAA TTGCTGGTGT CTCGGCTGTC 
CTCGGTGGGG TGATCGCTCT AAATGTAGAG GAAGCTGTCT CAGGTCCCCA TCTCTCGGTG 
ACGTTCTTTT GGATTTTAGT GGCTTGTTTT CCAAGTGCCA TCGCCAGCCA TGTGACAGCG 
GAATGTCCCA GCAAATGTCT GGTGGAGGTC CTGATCACCA TCAGCAGCCT TACGTCCCCA 
CTGCTGTTCA CCGCCTCTGG ATACCTGTCC TTCAGCGTGA TGAGAATCGT GGAGATTTTT 
AAAGATTACC CACCAGACAT CAAATCCTAT GATGTGCTGC TGCTGCTCCT GCTGCTGCTG 
CTGCTGCTTC AGGGGGGACT CAACACTGGC ACCGCCATCC AGTGTGTGAG CTTCAAAGTC 
AGCGCCCGGC TGCAGGCGGC CTCCTGGGAC TCCCAGTCCT GTCCCCAGGA GCGGCCAGCA 
GGCGAGGTGG TCCGAAGCCC CCTCAAGGAG TTTGACAAAG AAAAAGCCTG GAGAGCTGTG 
GTGGTACAAA TGGCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mlc1 ( XM_017594878.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450367.1 Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62021
Clone ID Related Accession (Same CDS sequence)	XM_017594878.1
Accession Version	XM_017594878.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	membrane protein MLC1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTATTC TCTGTGTTGA TGGGGAGCTG CCTTCTCGTG ACCTCTGGGT TTTCGCTCTA 
TCTGGGGAAC GTGTTCCCCT CCGAGATGGA TTATTTGCGC TGTGCAGCAG GCTCTGTAAG 
CAAACCCCTC AGCCACATCG GCACCATAAT CCTCCCACTT GCATCCCCTC AGCAATCGTG 
AGCTTCGCTG TTGGGAGGAG AAATGTCAGT GCGATTCCCA ACTTTCAGAT ATTGTTCGTT 
TCCACGTTCG CTGTTACCAC TACGTGCTTA ATCTGGTTTG GATGCAAATT GATCCTGAAT 
CCTTCAGCCA TAAATATTAA TTTCAATCTC ATCCTGCTCT TGCTGTTGGA GCTCCTCATG 
GCTGCCACGG TGATCATCTC GGCCCGGTCC AGTGAGGAGC CCTGCAAGAA GAAGAAGGGT 
TCCATCTCGG ATGGCACTAA CATTCTGGAT GAAGTGACTT TTCCTGCTCG GGTCCTAAAG 
TCCTATTCTG TGGTAGAAGT AATTGCTGGT GTCTCGGCTG TCCTCGGTGG GGTGATCGCT 
CTAAATGTAG AGGAAGCTGT CTCAGGTCCC CATCTCTCGG TGACGTTCTT TTGGATTTTA 
GTGGCTTGTT TTCCAAGTGC CATCGCCAGC CATGTGACAG CGGAATGTCC CAGCAAATGT 
CTGGTGGAGG TCCTGATCAC CATCAGCAGC CTTACGTCCC CACTGCTGTT CACCGCCTCT 
GGATACCTGT CCTTCAGCGT GATGAGAATC GTGGAGATTT TTAAAGATTA CCCACCAGAC 
ATCAAATCCT ATGATGTGCT GCTGCTGCTC CTGCTGCTGC TGCTGCTGCT TCAGGGGGGA 
CTCAACACTG GCACCGCCAT CCAGTGTGTG AGCTTCAAAG TCAGCGCCCG GCTGCAGGCG 
GCCTCCTGGG ACTCCCAGTC CTGTCCCCAG GAGCGGCCAG CAGGCGAGGT GGTCCGAAGC 
CCCCTCAAGG AGTTTGACAA AGAAAAAGCC TGGAGAGCTG TGGTGGTACA AATGGCCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450367.1
CDS	300..1322
Translation	MGILCVDGELPSRDLWVFALSGERVPLRDGLFALCSRLCKQTPQPHRHHNPPTCIPSAIVSFAVGRRNVSAIPNFQILFVSTFAVTTTCLIWFGCKLILNPSAININFNLILLLLLELLMAATVIISARSSEEPCKKKKGSISDGTNILDEVTFPARVLKSYSVVEVIAGVSAVLGGVIALNVEEAVSGPHLSVTFFWILVACFPSAIASHVTAECPSKCLVEVLITISSLTSPLLFTASGYLSFSVMRIVEIFKDYPPDIKSYDVLLLLLLLLLLLQGGLNTGTAIQCVSFKVSARLQAASWDSQSCPQERPAGEVVRSPLKEFDKEKAWRAVVVQMAQ

Target ORF information:

RefSeq Version	XM_017594878.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594878.1

ORF Insert Sequence:

ATGGGTATTC TCTGTGTTGA TGGGGAGCTG CCTTCTCGTG ACCTCTGGGT TTTCGCTCTA 
TCTGGGGAAC GTGTTCCCCT CCGAGATGGA TTATTTGCGC TGTGCAGCAG GCTCTGTAAG 
CAAACCCCTC AGCCACATCG GCACCATAAT CCTCCCACTT GCATCCCCTC AGCAATCGTG 
AGCTTCGCTG TTGGGAGGAG AAATGTCAGT GCGATTCCCA ACTTTCAGAT ATTGTTCGTT 
TCCACGTTCG CTGTTACCAC TACGTGCTTA ATCTGGTTTG GATGCAAATT GATCCTGAAT 
CCTTCAGCCA TAAATATTAA TTTCAATCTC ATCCTGCTCT TGCTGTTGGA GCTCCTCATG 
GCTGCCACGG TGATCATCTC GGCCCGGTCC AGTGAGGAGC CCTGCAAGAA GAAGAAGGGT 
TCCATCTCGG ATGGCACTAA CATTCTGGAT GAAGTGACTT TTCCTGCTCG GGTCCTAAAG 
TCCTATTCTG TGGTAGAAGT AATTGCTGGT GTCTCGGCTG TCCTCGGTGG GGTGATCGCT 
CTAAATGTAG AGGAAGCTGT CTCAGGTCCC CATCTCTCGG TGACGTTCTT TTGGATTTTA 
GTGGCTTGTT TTCCAAGTGC CATCGCCAGC CATGTGACAG CGGAATGTCC CAGCAAATGT 
CTGGTGGAGG TCCTGATCAC CATCAGCAGC CTTACGTCCC CACTGCTGTT CACCGCCTCT 
GGATACCTGT CCTTCAGCGT GATGAGAATC GTGGAGATTT TTAAAGATTA CCCACCAGAC 
ATCAAATCCT ATGATGTGCT GCTGCTGCTC CTGCTGCTGC TGCTGCTGCT TCAGGGGGGA 
CTCAACACTG GCACCGCCAT CCAGTGTGTG AGCTTCAAAG TCAGCGCCCG GCTGCAGGCG 
GCCTCCTGGG ACTCCCAGTC CTGTCCCCAG GAGCGGCCAG CAGGCGAGGT GGTCCGAAGC 
CCCCTCAAGG AGTTTGACAA AGAAAAAGCC TGGAGAGCTG TGGTGGTACA AATGGCCCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GlialCAM/MLC1 modulates LRRC8/VRAC currents in an indirect manner: Implications for megalencephalic leukoencephalopathy.
Neurobiology of disease11988-99(2018 11)
Elorza-Vidal X,Sirisi S,Gaitán-Peñas H,Pérez-Rius C,Alonso-Gardón M,Armand-Ugón M,Lanciotti A,Brignone MS,Prat E,Nunes V,Ambrosini E,Gasull X,Estévez R

Megalencephalic leukoencephalopathy with subcortical cysts protein-1 regulates epidermal growth factor receptor signaling in astrocytes.
Human molecular genetics25(8)1543-58(2016 Apr)
Lanciotti A,Brignone MS,Visentin S,De Nuccio C,Catacuzzeno L,Mallozzi C,Petrini S,Caramia M,Veroni C,Minnone G,Bernardo A,Franciolini F,Pessia M,Bertini E,Petrucci TC,Ambrosini E

Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations.
Human molecular genetics21(10)2166-80(2012 May)
Lanciotti A,Brignone MS,Molinari P,Visentin S,De Nuccio C,Macchia G,Aiello C,Bertini E,Aloisi F,Petrucci TC,Ambrosini E

MLC1 trafficking and membrane expression in astrocytes: role of caveolin-1 and phosphorylation.
Neurobiology of disease37(3)581-95(2010 Mar)
Lanciotti A,Brignone MS,Camerini S,Serafini B,Macchia G,Raggi C,Molinari P,Crescenzi M,Musumeci M,Sargiacomo M,Aloisi F,Petrucci TC,Ambrosini E

Biochemical characterization of MLC1 protein in astrocytes and its association with the dystrophin-glycoprotein complex.
Molecular and cellular neurosciences37(3)480-93(2008 Mar)
Ambrosini E,Serafini B,Lanciotti A,Tosini F,Scialpi F,Psaila R,Raggi C,Di Girolamo F,Petrucci TC,Aloisi F

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