Arfgef1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgef1
Entrez Gene ID	312915
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms	BIG1,RGD1560793
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 1 Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) brefeldin A-inhibited GEP 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001277056.1	NP_001263985.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1
XM_017593322.1	XP_017448811.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
Macaca mulatta (Rhesus monkey)	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Neurospora crassa	NCU07658	XP_962785.2
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3

Gene Ontology
Bibliography

Related articles in PubMed

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange factor, is required for GABA-gated Cl⁻ influx through regulation of GABAA receptor trafficking.
Li C, Chen S, Yu Y, Zhou C, Wang Y, Le K, Li D, Shao W, Lu L, You Y, Peng J, Huang H, Liu P, Shen X
Molecular neurobiology49(2)808-19(2014 Apr)

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein regulates neurite development via PI3K-AKT and ERK signaling pathways.
Zhou C, Li C, Li D, Wang Y, Shao W, You Y, Peng J, Zhang X, Lu L, Shen X
Neuroscience254361-8(2013 Dec)

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.
Lin S, Zhou C, Neufeld E, Wang YH, Xu SW, Lu L, Wang Y, Liu ZP, Li D, Li C, Chen S, Le K, Huang H, Liu P, Moss J, Vaughan M, Shen X
Arteriosclerosis, thrombosis, and vascular biology33(2)e31-8(2013 Feb)

Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing.
Li CC, Kuo JC, Waterman CM, Kiyama R, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America108(48)19228-33(2011 Nov)

Specific functions of BIG1 and BIG2 in endomembrane organization.
Boal F, Stephens DJ
PloS one5(3)e9898(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11581	NM_001277056.1 Latest version!	Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61200	XM_017593322.1 Latest version!	Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgef1 ( NM_001277056.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263985.1 Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), mRNA.

Price & Availability↑

CloneID	ORa11581
Clone ID Related Accession (Same CDS sequence)	NM_001277056.1
Accession Version	NM_001277056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Rattus norvegicus(Norway rat)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07046830.1. On or before Mar 2, 2013 this sequence version replaced XM_001066125.3, XM_232614.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GACTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGTGCTCC AGATAGTACG 
ACACCAGGCA AAAAGTTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGC 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA CGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCATCAC TTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AGCCCAAGAA 
CAGGAAGGGG ATCTTGATCC CCAAACACAC GATGTGGATA AAAGTCTCCA AGATGACATA 
GAGCCTGAGA ACGGATCTGA TATTTCCAGT GCAGAGAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAGAT GATGTGTTGT GTGATGGAGA GTGTGAGGAA 
AAGCCACAGG ATATTGTGCA GAGCATTGTG GAAGAGATGG TGGACATCAT TGTTGGAGAC 
ATGGGGGAAG GAACAGCTGT AAGTGCAAGT GCAGATGGCA ACGCTGGAGC TGTAGAGGAT 
GGCAGTGACA GTGAGAACGT GCAAGCCAAT GGGATCCCGG GAACACCCAT CTCTGCCGCA 
TACACACCAT CGTTACCTGA CGACAGATTG TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTGTGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTAAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT TTTCCTGTAC ATTTTGGAAA CTTCTACAAG CTCATTTGAT 
CATAAGTGGA TGGTGATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GATATTTATG TAAATTATGA CTGTGACTTG AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CAAAAATTGC CCAAGGAAGG GGCAGTCAAG AACTTGGGAT GAGTAATGTT 
CAGGAATTGA GCCTGCGGAA AAAGGGGTTA GAATGTTTAG TTTCAATTCT TAAGTGTATG 
GTTGAATGGA GTAAGGATCA GTACGTGAAT CCCAACTCTC AAACCACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAATC AAACACCCCG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCT TCAGGGATTG GCAGCTACAG CACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTCGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAGTACCT CCAAGAACAA 
GGGATGCTTG GCACCACTCC TGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACAC AGGCGGGGGA GTTCCTGGGA GATAACGATA AGTTCAATAA GGAAGTCATG 
TATGCATATG TAGACCAGCA TGACTTTTCG GGAAAAGACT TTGTTTCAGC TCTTCGTCTG 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACCACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACGAAAGA ACAGTATATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCTGCCATT TATAATGAGA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAATCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAATTTA GAAATGGAAC AGATGGCCAA GACAGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CTATGTTTAA GTTGGCTTGG ACCCCTTTTC TGGCTGCCTT CAGTGTGGGT 
CTACAAGACT GTGATGATAC TGACGTGGCC TCTCTCTGCC TCGAAGGGAT ACGATGTGCG 
ATCAGAATTG CATGCATCTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAGGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACAGTGGCT CACACAGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATTAGTCAG TTAGAGCTTG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACCGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCGGATGA ATTTGTTGGT CTGGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GTTCTCAAAG TGTCGTGGTT 
GCTGTAGACA GAATATTCAC AGGATCCACA AGGCTAGATG GAAATGCTAT TGTCGATTTT 
GTTCGTTGGC TCTGTGCCGT GTCTATGGAT GAATTGCTTT CCACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCC CTGAGGCAGC TATCGATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TAAGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTCG TGCGTTGTAT AGCGCAGATG 
GTTAACTCTC AGGCTGCCAA TATCCGTTCA GGATGGAAGA ACATTTTCTC GGTGTTCCAC 
CTAGCTGCAT CAGACCAGGA TGAGAGCATA GTAGAGCTAG CATTCCAGAC GAGCGGGCAC 
ATCGTCACTC TTGTATTTGA AAAGCACTTT CCAGCCACTA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TATCTGAATT TGCGTGTAAT GCGGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCTCAGGC TTTCAAAGAG 
TACACAAGTG ATGACATGAA CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCAATCCTCT TTGAGTTGTC CTGTGTCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TGATGTTTGA AATAATGAAA ACATACGGCC ACACTTACGA AAAACACTGG 
TGGCAGGACT TGTTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAGTT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCGATATGC 
GACGTGTTCA CCCAGTATTT AGAAGTCCTC AGTGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACGAACTGC 
TTAGAAAATG TGGTTATTCT GAATGGTGAG AAGTTTACTC TAGAAATCTG GGATAAAACC 
TGTAATTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ATGCGCTTCT GACTTGGCGT 
CCTACTTCTG GAGAGGCTGC CCCCCCATCT CCATCAGCTA TGAGTGAAAA GCAGTTGGAT 
GCAATATCAC AGAAATCTGT AGACATACAT GATTCTGCTC AACCAAGATC TTCAGATAAC 
AGGCAGCAAG CACCACTTGT TTCTGTTTCT CCTGCGAGTG AAGAAGTTAG CAAAGGGAGA 
CCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTGTTCT TCCCAGCCAC AAGCAAGAAG 
GAAGATGCAG AAAACTTAGC TGCAGCCCAG AGAGATGCAG TGGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTCTT CAAGCTACTG 
GACTGCTTAT TAGAGTCACA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTAT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTCCT AAAACAAGAG 
ACAAGCAGCC TGGCGTGTGG ACTGCGCATC CTCTTCCGGA TGTACACAGA CGAGAGCAGA 
GTCAGCGCCT GGGAGGAGGT CCAGCAGAGG CTTTTAAACG TCTGCAGTGA GGCACTGAGT 
TACTTCCTCA CTCTAACATC TGAAAGTCAC CGGGAAGCCT GGACCAACTT ACTGTTACTG 
TTCCTCACTA AAGTCCTCAA GATCAGTGAC AACAGGTTTA AAGCTCACGC ATCATTCTAC 
TACCCTCTGT TATGTGAAAT TATGCAATTT GACTTGATTC CTGAACTTCG TGCTGTTCTT 
AGAAGATTTT TCCTGCGAAT TGGAATAGTA TTTCAGATAT CACAACCACC TGAACAGGAA 
CTTGGGATAA ACAAGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263985.1
CDS	157..5697
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGSAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQHHLLQSPVSHHEPESPHLRYLPPQTVDHIAQEQEGDLDPQTHDVDKSLQDDIEPENGSDISSAENEQTEADQATAAETLSKDDVLCDGECEEKPQDIVQSIVEEMVDIIVGDMGEGTAVSASADGNAGAVEDGSDSENVQANGIPGTPISAAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQAGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTSGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCVINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPTSGEAAPPSPSAMSEKQLDAISQKSVDIHDSAQPRSSDNRQQAPLVSVSPASEEVSKGRPTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGIVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	NM_001277056.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277056.1

ORF Insert Sequence:

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GACTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGTGCTCC AGATAGTACG 
ACACCAGGCA AAAAGTTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGC 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA CGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCATCAC TTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AGCCCAAGAA 
CAGGAAGGGG ATCTTGATCC CCAAACACAC GATGTGGATA AAAGTCTCCA AGATGACATA 
GAGCCTGAGA ACGGATCTGA TATTTCCAGT GCAGAGAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAGAT GATGTGTTGT GTGATGGAGA GTGTGAGGAA 
AAGCCACAGG ATATTGTGCA GAGCATTGTG GAAGAGATGG TGGACATCAT TGTTGGAGAC 
ATGGGGGAAG GAACAGCTGT AAGTGCAAGT GCAGATGGCA ACGCTGGAGC TGTAGAGGAT 
GGCAGTGACA GTGAGAACGT GCAAGCCAAT GGGATCCCGG GAACACCCAT CTCTGCCGCA 
TACACACCAT CGTTACCTGA CGACAGATTG TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTGTGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTAAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT TTTCCTGTAC ATTTTGGAAA CTTCTACAAG CTCATTTGAT 
CATAAGTGGA TGGTGATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GATATTTATG TAAATTATGA CTGTGACTTG AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CAAAAATTGC CCAAGGAAGG GGCAGTCAAG AACTTGGGAT GAGTAATGTT 
CAGGAATTGA GCCTGCGGAA AAAGGGGTTA GAATGTTTAG TTTCAATTCT TAAGTGTATG 
GTTGAATGGA GTAAGGATCA GTACGTGAAT CCCAACTCTC AAACCACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAATC AAACACCCCG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCT TCAGGGATTG GCAGCTACAG CACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTCGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAGTACCT CCAAGAACAA 
GGGATGCTTG GCACCACTCC TGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACAC AGGCGGGGGA GTTCCTGGGA GATAACGATA AGTTCAATAA GGAAGTCATG 
TATGCATATG TAGACCAGCA TGACTTTTCG GGAAAAGACT TTGTTTCAGC TCTTCGTCTG 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACCACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACGAAAGA ACAGTATATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCTGCCATT TATAATGAGA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAATCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAATTTA GAAATGGAAC AGATGGCCAA GACAGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CTATGTTTAA GTTGGCTTGG ACCCCTTTTC TGGCTGCCTT CAGTGTGGGT 
CTACAAGACT GTGATGATAC TGACGTGGCC TCTCTCTGCC TCGAAGGGAT ACGATGTGCG 
ATCAGAATTG CATGCATCTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAGGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACAGTGGCT CACACAGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATTAGTCAG TTAGAGCTTG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACCGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCGGATGA ATTTGTTGGT CTGGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GTTCTCAAAG TGTCGTGGTT 
GCTGTAGACA GAATATTCAC AGGATCCACA AGGCTAGATG GAAATGCTAT TGTCGATTTT 
GTTCGTTGGC TCTGTGCCGT GTCTATGGAT GAATTGCTTT CCACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCC CTGAGGCAGC TATCGATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TAAGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTCG TGCGTTGTAT AGCGCAGATG 
GTTAACTCTC AGGCTGCCAA TATCCGTTCA GGATGGAAGA ACATTTTCTC GGTGTTCCAC 
CTAGCTGCAT CAGACCAGGA TGAGAGCATA GTAGAGCTAG CATTCCAGAC GAGCGGGCAC 
ATCGTCACTC TTGTATTTGA AAAGCACTTT CCAGCCACTA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TATCTGAATT TGCGTGTAAT GCGGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCTCAGGC TTTCAAAGAG 
TACACAAGTG ATGACATGAA CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCAATCCTCT TTGAGTTGTC CTGTGTCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TGATGTTTGA AATAATGAAA ACATACGGCC ACACTTACGA AAAACACTGG 
TGGCAGGACT TGTTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAGTT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCGATATGC 
GACGTGTTCA CCCAGTATTT AGAAGTCCTC AGTGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACGAACTGC 
TTAGAAAATG TGGTTATTCT GAATGGTGAG AAGTTTACTC TAGAAATCTG GGATAAAACC 
TGTAATTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ATGCGCTTCT GACTTGGCGT 
CCTACTTCTG GAGAGGCTGC CCCCCCATCT CCATCAGCTA TGAGTGAAAA GCAGTTGGAT 
GCAATATCAC AGAAATCTGT AGACATACAT GATTCTGCTC AACCAAGATC TTCAGATAAC 
AGGCAGCAAG CACCACTTGT TTCTGTTTCT CCTGCGAGTG AAGAAGTTAG CAAAGGGAGA 
CCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTGTTCT TCCCAGCCAC AAGCAAGAAG 
GAAGATGCAG AAAACTTAGC TGCAGCCCAG AGAGATGCAG TGGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTCTT CAAGCTACTG 
GACTGCTTAT TAGAGTCACA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTAT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTCCT AAAACAAGAG 
ACAAGCAGCC TGGCGTGTGG ACTGCGCATC CTCTTCCGGA TGTACACAGA CGAGAGCAGA 
GTCAGCGCCT GGGAGGAGGT CCAGCAGAGG CTTTTAAACG TCTGCAGTGA GGCACTGAGT 
TACTTCCTCA CTCTAACATC TGAAAGTCAC CGGGAAGCCT GGACCAACTT ACTGTTACTG 
TTCCTCACTA AAGTCCTCAA GATCAGTGAC AACAGGTTTA AAGCTCACGC ATCATTCTAC 
TACCCTCTGT TATGTGAAAT TATGCAATTT GACTTGATTC CTGAACTTCG TGCTGTTCTT 
AGAAGATTTT TCCTGCGAAT TGGAATAGTA TTTCAGATAT CACAACCACC TGAACAGGAA 
CTTGGGATAA ACAAGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgef1 ( XM_017593322.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448811.1 Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), transcript variant X1, mRNA.

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CloneID	ORa61200
Clone ID Related Accession (Same CDS sequence)	XM_017593322.1
Accession Version	XM_017593322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GACTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGTGCTCC AGATAGTACG 
ACACCAGGCA AAAAGTTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGC 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA CGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCATCAC TTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AGCCCAAGAA 
CAGGAAGGGG ATCTTGATCC CCAAACACAC GATGTGGATA AAAGTCTCCA AGATGACATA 
GAGCCTGAGA ACGGATCTGA TATTTCCAGT GCAGAGAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAGAT GATGTGTTGT GTGATGGAGA GTGTGAGGAA 
AAGCCACAGG ATATTGTGCA GAGCATTGTG GAAGAGATGG TGGACATCAT TGTTGGAGAC 
ATGGGGGAAG GAACAGCTGT AAGTGCAAGT GCAGATGGCA ACGCTGGAGC TGTAGAGGAT 
GGCAGTGACA GTGAGAACGT GCAAGCCAAT GGGATCCCGG GAACACCCAT CTCTGCCGCA 
TACACACCAT CGTTACCTGA CGACAGATTG TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTGTGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTAAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT TTTCCTGTAC ATTTTGGAAA CTTCTACAAG CTCATTTGAT 
CATAAGTGGA TGGTGATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GATATTTATG TAAATTATGA CTGTGACTTG AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CAAAAATTGC CCAAGGAAGG GGCAGTCAAG AACTTGGGAT GAGTAATGTT 
CAGGAATTGA GCCTGCGGAA AAAGGGGTTA GAATGTTTAG TTTCAATTCT TAAGTGTATG 
GTTGAATGGA GTAAGGATCA GTACGTGAAT CCCAACTCTC AAACCACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAATC AAACACCCCG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCT TCAGGGATTG GCAGCTACAG CACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTCGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAGTACCT CCAAGAACAA 
GGGATGCTTG GCACCACTCC TGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACAC AGGCGGGGGA GTTCCTGGGA GATAACGATA AGTTCAATAA GGAAGTCATG 
TATGCATATG TAGACCAGCA TGACTTTTCG GGAAAAGACT TTGTTTCAGC TCTTCGTCTG 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACCACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACGAAAGA ACAGTATATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCTGCCATT TATAATGAGA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAATCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAATTTA GAAATGGAAC AGATGGCCAA GACAGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CTATGTTTAA GTTGGCTTGG ACCCCTTTTC TGGCTGCCTT CAGTGTGGGT 
CTACAAGACT GTGATGATAC TGACGTGGCC TCTCTCTGCC TCGAAGGGAT ACGATGTGCG 
ATCAGAATTG CATGCATCTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAGGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACAGTGGCT CACACAGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATTAGTCAG TTAGAGCTTG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACCGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCGGATGA ATTTGTTGGT CTGGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GTTCTCAAAG TGTCGTGGTT 
GCTGTAGACA GAATATTCAC AGGATCCACA AGGCTAGATG GAAATGCTAT TGTCGATTTT 
GTTCGTTGGC TCTGTGCCGT GTCTATGGAT GAATTGCTTT CCACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCC CTGAGGCAGC TATCGATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TAAGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTCG TGCGTTGTAT AGCGCAGATG 
GTTAACTCTC AGGCTGCCAA TATCCGTTCA GGATGGAAGA ACATTTTCTC GGTGTTCCAC 
CTAGCTGCAT CAGACCAGGA TGAGAGCATA GTAGAGCTAG CATTCCAGAC GAGCGGGCAC 
ATCGTCACTC TTGTATTTGA AAAGCACTTT CCAGCCACTA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TATCTGAATT TGCGTGTAAT GCGGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCTCAGGC TTTCAAAGAG 
TACACAAGTG ATGACATGAA CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCAATCCTCT TTGAGTTGTC CTGTGTCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TGATGTTTGA AATAATGAAA ACATACGGCC ACACTTACGA AAAACACTGG 
TGGCAGGACT TGTTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAGTT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCGATATGC 
GACGTGTTCA CCCAGTATTT AGAAGTCCTC AGTGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACGAACTGC 
TTAGAAAATG TGGTTATTCT GAATGGTGAG AAGTTTACTC TAGAAATCTG GGATAAAACC 
TGTAATTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ATGCGCTTCT GACTTGGCGT 
CCTACTTCTG GAGAGGCTGC CCCCCCATCT CCATCAGCTA TGAGTGAAAA GCAGTTGGAT 
GCAATATCAC AGAAATCTGT AGACATACAT GATTCTGCTC AACCAAGATC TTCAGATAAC 
AGGCAGCAAG CACCACTTGT TTCTGTTTCT CCTGCGAGTG AAGAAGTTAG CAAAGGGAGA 
CCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTGTTCT TCCCAGCCAC AAGCAAGAAG 
GAAGATGCAG AAAACTTAGC TGCAGCCCAG AGAGATGCAG TGGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTCTT CAAGCTACTG 
GACTGCTTAT TAGAGTCACA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTAT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTCCT AAAACAAGAG 
ACAAGCAGCC TGGCGTGTGG ACTGCGCATC CTCTTCCGGA TGTACACAGA CGAGAGCAGA 
GTCAGCGCCT GGGAGGAGGT CCAGCAGAGG CTTTTAAACG TCTGCAGTGA GGCACTGAGT 
TACTTCCTCA CTCTAACATC TGAAAGTCAC CGGGAAGCCT GGACCAACTT ACTGTTACTG 
TTCCTCACTA AAGTCCTCAA GATCAGTGAC AACAGGGAAA AGCATGCTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448811.1
CDS	311..5704
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGSAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMERERHRQQHHLLQSPVSHHEPESPHLRYLPPQTVDHIAQEQEGDLDPQTHDVDKSLQDDIEPENGSDISSAENEQTEADQATAAETLSKDDVLCDGECEEKPQDIVQSIVEEMVDIIVGDMGEGTAVSASADGNAGAVEDGSDSENVQANGIPGTPISAAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQEISEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQAGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTSGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCVINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPTSGEAAPPSPSAMSEKQLDAISQKSVDIHDSAQPRSSDNRQQAPLVSVSPASEEVSKGRPTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNREKHAN

Target ORF information:

RefSeq Version	XM_017593322.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP ribosylation factor guanine nucleotide exchange factor 1 (Arfgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593322.1

ORF Insert Sequence:

ATGTATGAGG GCAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCC 
CTAGAGGAAA TAAAAGTTGA AACTGAGAAA CAAAGTCCTC CTCATGGAGA AGCAAAAGCT 
GGATCAGGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCTTACCCT TTGAGTTGGC ATGCCAGTCC AAATGTCCCC GCATAGTTAG TACATCTCTA 
GACTGTTTGC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAGTGCTCC AGATAGTACG 
ACACCAGGCA AAAAGTTAAT TGATAGAATT ATTGAAACAA TATGTGGCTG CTTCCAAGGC 
CCTCAAACAG ATGAAGGAGT TCAGTTGCAG ATAATAAAGG CTTTACTTAC TGCAGTAACA 
TCACAACACA TAGAAATTCA CGAAGGAACT GTGCTGCAAG CTGTGAGGAC ATGCTACAAT 
ATCTATTTAG CCAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGCTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TACAGGAAGC CAAACAAATG 
GAAAGAGAAA GACATCGGCA GCAGCATCAC TTGTTACAGT CTCCAGTAAG CCATCACGAG 
CCCGAGTCAC CTCATCTTAG ATATTTGCCA CCTCAGACTG TTGATCATAT AGCCCAAGAA 
CAGGAAGGGG ATCTTGATCC CCAAACACAC GATGTGGATA AAAGTCTCCA AGATGACATA 
GAGCCTGAGA ACGGATCTGA TATTTCCAGT GCAGAGAATG AACAGACAGA AGCTGATCAG 
GCAACTGCAG CTGAAACATT ATCTAAAGAT GATGTGTTGT GTGATGGAGA GTGTGAGGAA 
AAGCCACAGG ATATTGTGCA GAGCATTGTG GAAGAGATGG TGGACATCAT TGTTGGAGAC 
ATGGGGGAAG GAACAGCTGT AAGTGCAAGT GCAGATGGCA ACGCTGGAGC TGTAGAGGAT 
GGCAGTGACA GTGAGAACGT GCAAGCCAAT GGGATCCCGG GAACACCCAT CTCTGCCGCA 
TACACACCAT CGTTACCTGA CGACAGATTG TCTGTCTCCT CCAATGATAC TCAGGAATCT 
GGAAATTCTT CAGGACCTTC ACCTGGTGCT AAGTTTTCCC ACATTTTACA AAAGGATGCC 
TTTCTAGTGT TCAGGTCATT GTGTAAGCTG TCAATGAAAC CACTGTCAGA TGGACCACCA 
GATCCAAAGT CTCATGAGTT ACGGTCCAAG ATTCTTTCAT TGCAGTTGCT TCTGTCCATT 
CTGCAGAATG CAGGACCTGT TTTCAGGACA AATGAGATGT TTATTAATGC TATCAAGCAG 
TATCTGTGTG TTGCACTCTC AAAAAACGGA GTCTCATCTG TTCCAGAGGT TTTTGAGCTT 
TCTCTTTCCA TATTTCTAAC TTTGTTGTCA AACTTCAAGA CACATCTGAA GATGCAAATT 
GAGGTATTCT TTAAAGAAAT TTTCCTGTAC ATTTTGGAAA CTTCTACAAG CTCATTTGAT 
CATAAGTGGA TGGTGATTCA GACACTGACA AGGATCTGTG CAGATGCTCA GAGTGTGGTG 
GATATTTATG TAAATTATGA CTGTGACTTG AATGCAGCAA ATATATTTGA AAGACTAGTA 
AATGACCTGT CAAAAATTGC CCAAGGAAGG GGCAGTCAAG AACTTGGGAT GAGTAATGTT 
CAGGAATTGA GCCTGCGGAA AAAGGGGTTA GAATGTTTAG TTTCAATTCT TAAGTGTATG 
GTTGAATGGA GTAAGGATCA GTACGTGAAT CCCAACTCTC AAACCACCCT TGGTCAGGAA 
AAACCCTCAG AGCAAGAGAT AAGTGAAATC AAACACCCCG AGACTATAAA CAGATATGGA 
AGTTTAAACT CCTTAGAATC CACATCATCT TCAGGGATTG GCAGCTACAG CACACAGATG 
TCTGGCACTG ATAATCCAGA ACAATTCGAG GTCCTAAAGC AGCAAAAAGA AATAATAGAA 
CAAGGGATTG ATTTATTTAA TAAGAAACCA AAGAGAGGAA TACAGTACCT CCAAGAACAA 
GGGATGCTTG GCACCACTCC TGAAGATATA GCTCAGTTCT TACATCAAGA AGAAAGATTA 
GACTCTACAC AGGCGGGGGA GTTCCTGGGA GATAACGATA AGTTCAATAA GGAAGTCATG 
TATGCATATG TAGACCAGCA TGACTTTTCG GGAAAAGACT TTGTTTCAGC TCTTCGTCTG 
TTTCTGGAAG GATTCCGGCT TCCAGGAGAA GCTCAGAAAA TTGATCGATT AATGGAAAAA 
TTTGCTGCAA GATACCTAGA GTGCAACCAA GGACAAACCC TTTTTGCTAG TGCAGATACT 
GCTTATGTTT TGGCCTACTC AATTATCATG TTGACCACAG ACCTTCACAG TCCACAGGTT 
AAAAACAAAA TGACGAAAGA ACAGTATATT AAAATGAATC GTGGTATCAA TGACAGTAAA 
GATCTCCCTG AAGAGTATCT ATCTGCCATT TATAATGAGA TAGCTGGGAA AAAAATATCA 
ATGAAAGAAA CAAAAGAACT AACAATCCCT ACAAAATCAA CTAAACAGAA TGTAGCCAGT 
GAAAAGCAAA GACGACTTCT GTATAATTTA GAAATGGAAC AGATGGCCAA GACAGCTAAA 
GCACTCATGG AAGCTGTGAG CCATGTTCAG GCACCTTTTA CAAGTGCAAC ACACTTGGAG 
CACGTGAGGC CTATGTTTAA GTTGGCTTGG ACCCCTTTTC TGGCTGCCTT CAGTGTGGGT 
CTACAAGACT GTGATGATAC TGACGTGGCC TCTCTCTGCC TCGAAGGGAT ACGATGTGCG 
ATCAGAATTG CATGCATCTT CAGCATTCAG CTGGAAAGAG ATGCATATGT CCAGGCACTA 
GCCAGATTTA CCTTACTTAC AGTGAGTTCT GGTATTACTG AAATGAAACA GAAGAATATC 
GACACAATAA AAACACTCAT CACAGTGGCT CACACAGATG GAAATTATTT AGGAAATTCG 
TGGCATGAGA TCCTGAAGTG CATTAGTCAG TTAGAGCTTG CACAACTTAT AGGAACTGGA 
GTGAAGCCTC GGTACATTTC TGGGACCGTG AGAGGCAGAG AAGGGTCTCT CACTGGGACA 
AAAGATCAGG CTCCGGATGA ATTTGTTGGT CTGGGGCTAG TTGGAGGAAA TGTGGACTGG 
AAGCAGATAG CCAGTATTCA GGAGTCTATT GGAGAAACCA GTTCTCAAAG TGTCGTGGTT 
GCTGTAGACA GAATATTCAC AGGATCCACA AGGCTAGATG GAAATGCTAT TGTCGATTTT 
GTTCGTTGGC TCTGTGCCGT GTCTATGGAT GAATTGCTTT CCACTACACA CCCAAGAATG 
TTTAGTCTAC AAAAAATAGT AGAAATATCA TACTATAACA TGGGAAGAAT AAGACTGCAG 
TGGTCTCGGA TTTGGGAAGT TATTGGAGAT CATTTTAATA AGGTTGGTTG CAATCCTAAT 
GAAGATGTAG CCATATTTGC AGTAGACTCC CTGAGGCAGC TATCGATGAA GTTTTTAGAG 
AAAGGAGAGC TTGCAAACTT CAGATTCCAA AAGGATTTCT TAAGACCTTT TGAACATATA 
ATGAAACGAA ACAGGTCTCC AACAATTCGA GATATGGTCG TGCGTTGTAT AGCGCAGATG 
GTTAACTCTC AGGCTGCCAA TATCCGTTCA GGATGGAAGA ACATTTTCTC GGTGTTCCAC 
CTAGCTGCAT CAGACCAGGA TGAGAGCATA GTAGAGCTAG CATTCCAGAC GAGCGGGCAC 
ATCGTCACTC TTGTATTTGA AAAGCACTTT CCAGCCACTA TTGATTCTTT CCAGGATGCA 
GTGAAGTGTT TATCTGAATT TGCGTGTAAT GCGGCTTTCC CAGACACAAG TATGGAAGCA 
ATCCGACTTA TTCGCCATTG TGCAAAATAT GTGTCTGATA GACCTCAGGC TTTCAAAGAG 
TACACAAGTG ATGACATGAA CGTGGCACCT GAAGACAGGG TGTGGGTGAG AGGGTGGTTC 
CCAATCCTCT TTGAGTTGTC CTGTGTCATC AATAGATGCA AATTAGATGT AAGAACCAGG 
GGCTTAACAG TGATGTTTGA AATAATGAAA ACATACGGCC ACACTTACGA AAAACACTGG 
TGGCAGGACT TGTTCAGAAT TGTTTTCAGA ATCTTTGACA ACATGAAGTT GCCAGAACAG 
CAGACAGAGA AAGCTGAATG GATGACAACA ACTTGTAACC ATGCCCTTTA TGCGATATGC 
GACGTGTTCA CCCAGTATTT AGAAGTCCTC AGTGATGTAC TTTTGGATGA TATTTTTGCC 
CAGCTTTACT GGTGTGTACA ACAAGACAAT GAGCAGTTAG CACGATCTGG TACGAACTGC 
TTAGAAAATG TGGTTATTCT GAATGGTGAG AAGTTTACTC TAGAAATCTG GGATAAAACC 
TGTAATTGCA CCCTGGATAT CTTCAAAACC ACAATTCCTC ATGCGCTTCT GACTTGGCGT 
CCTACTTCTG GAGAGGCTGC CCCCCCATCT CCATCAGCTA TGAGTGAAAA GCAGTTGGAT 
GCAATATCAC AGAAATCTGT AGACATACAT GATTCTGCTC AACCAAGATC TTCAGATAAC 
AGGCAGCAAG CACCACTTGT TTCTGTTTCT CCTGCGAGTG AAGAAGTTAG CAAAGGGAGA 
CCCACAGCAA AATTCCCAGA ACAAAAATTG TTTGCTGCCC TGTTGATTAA ATGTGTTGTG 
CAGCTGGAAC TCATCCAGAC CATCGACAAC ATTGTGTTCT TCCCAGCCAC AAGCAAGAAG 
GAAGATGCAG AAAACTTAGC TGCAGCCCAG AGAGATGCAG TGGACTTCGA TGTTCGAGTT 
GATACTCAAG ACCAAGGAAT GTACCGCTTT TTAACATCAC AACAACTCTT CAAGCTACTG 
GACTGCTTAT TAGAGTCACA TAGATTTGCA AAAGCATTTA ATTCCAACAA TGAACAGAGG 
ACTGCTCTAT GGAAAGCAGG CTTCAAAGGC AAGTCCAAGC CCAATCTCCT AAAACAAGAG 
ACAAGCAGCC TGGCGTGTGG ACTGCGCATC CTCTTCCGGA TGTACACAGA CGAGAGCAGA 
GTCAGCGCCT GGGAGGAGGT CCAGCAGAGG CTTTTAAACG TCTGCAGTGA GGCACTGAGT 
TACTTCCTCA CTCTAACATC TGAAAGTCAC CGGGAAGCCT GGACCAACTT ACTGTTACTG 
TTCCTCACTA AAGTCCTCAA GATCAGTGAC AACAGGGAAA AGCATGCTAA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange factor, is required for GABA-gated Cl⁻ influx through regulation of GABAA receptor trafficking.
Molecular neurobiology49(2)808-19(2014 Apr)
Li C,Chen S,Yu Y,Zhou C,Wang Y,Le K,Li D,Shao W,Lu L,You Y,Peng J,Huang H,Liu P,Shen X

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein regulates neurite development via PI3K-AKT and ERK signaling pathways.
Neuroscience254361-8(2013 Dec)
Zhou C,Li C,Li D,Wang Y,Shao W,You Y,Peng J,Zhang X,Lu L,Shen X

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.
Arteriosclerosis, thrombosis, and vascular biology33(2)e31-8(2013 Feb)
Lin S,Zhou C,Neufeld E,Wang YH,Xu SW,Lu L,Wang Y,Liu ZP,Li D,Li C,Chen S,Le K,Huang H,Liu P,Moss J,Vaughan M,Shen X

Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing.
Proceedings of the National Academy of Sciences of the United States of America108(48)19228-33(2011 Nov)
Li CC,Kuo JC,Waterman CM,Kiyama R,Moss J,Vaughan M

Specific functions of BIG1 and BIG2 in endomembrane organization.
PloS one5(3)e9898(2010 Mar)
Boal F,Stephens DJ

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Arfgef1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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