arfgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arfgef1
Entrez Gene ID	100492065
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 1 Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012965257.2	XP_012820711.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
XM_012965257.3	XP_012820711.2	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
XM_012965258.2	XP_012820712.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
XM_012965258.3	XP_012820712.2	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
Macaca mulatta (Rhesus monkey)	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Neurospora crassa	NCU07658	XP_962785.2
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3

Gene Ontology

The following arfgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arfgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35255	XM_012965257.2 Latest version!	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35256	XM_012965258.2 Latest version!	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60863	XM_012965257.3 Latest version!	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60864	XM_012965258.3 Latest version!	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arfgef1 ( XM_012965257.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820711.1 Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa35255
Clone ID Related Accession (Same CDS sequence)	XM_012965257.2
Accession Version	XM_012965257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT 
AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG 
TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG 
GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT 
AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA 
GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA 
AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC 
ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA 
AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG 
AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA 
GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA 
GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT 
CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC 
AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC 
TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG 
GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA 
AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT 
GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA 
GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA 
GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA 
GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA 
GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG 
TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC 
AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC 
TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT 
ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA 
GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG 
GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG 
ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA 
TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG 
CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT 
GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC 
CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG 
CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT 
CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT 
CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA 
GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA 
AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT 
GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG 
TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC 
CACTTTAATA AGGTCGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT 
CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG 
AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA 
GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA 
GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA 
GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC 
CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT 
GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT 
GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA 
GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC 
AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA 
ACCTATGGGC ACACGTTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG 
ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT 
ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG 
AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT 
GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG 
AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC 
ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA 
CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT 
GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA 
GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA 
TTTGCAGCCT TGCTGATTAA GTGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC 
ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCGG AGAATCTGGC TGCGGCACAG 
AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC 
CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA 
AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG 
AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT 
CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA 
TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC 
AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGCGAT 
GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT 
GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT 
TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA 
CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820711.1
CDS	331..5910
Translation	MFLTRALDKILSDKEVKRAHHSQLRKACEVALEEIKCETEKQSSPQDESKSGSSTLPPVKSKATFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEDVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLVNQTTAKATLTQMLNVIFARMENQALQEAKHMEKERHKQQHHLQHSPVSHHELELPPMRHHLEQTDQLTREHEGAIDQHLNYMENSLQEEAEPENGYDAPSAENELLETNQCAENHSKADLEIGDETSIDCEESTQEVIQSIIQEMLNSVVGEEETVRAECTARISEDPSDGDSIHANGIPGTPISMAYIPSLPDDRLSVSSNDTQARKESGNTSAPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPVFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKLHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDLYVNPNSQTTLGQEKPSEQDTHDTKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTSPDDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFQGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKSKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELAITTKSSKPSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFCIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGSSWHEILKCISQLELAQLIGTGVKPRYISGTVRNREGSFTGTKDQASDEFVSLGLVGGNVDWKQMASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAVVDFVRWLCAVSMDELLSPMHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTFEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCMLDIFKTTIPHALLTWKPAGGDTELSSQADGRDKQADSVSQKSVDIHESIQPRTADSRRNIQTQVSLVAQEEGHLARPGARVAEQRLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDTVHLDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTSLWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDDSRSDAWDEVQHRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIIQFDLIPELRAILRRFFHRIGLVFKISIPQEQEPQRVEEKLEAPDSQYPEGRRKETVQPESF

Target ORF information:

RefSeq Version	XM_012965257.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965257.2

ORF Insert Sequence:

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT 
AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG 
TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG 
GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT 
AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA 
GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA 
AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC 
ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA 
AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG 
AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA 
GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA 
GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT 
CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC 
AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC 
TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG 
GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA 
AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT 
GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA 
GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA 
GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA 
GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA 
GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG 
TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC 
AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC 
TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT 
ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA 
GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG 
GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG 
ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA 
TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG 
CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT 
GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC 
CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG 
CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT 
CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT 
CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA 
GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA 
AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT 
GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG 
TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC 
CACTTTAATA AGGTCGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT 
CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG 
AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA 
GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA 
GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA 
GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC 
CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT 
GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT 
GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA 
GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC 
AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA 
ACCTATGGGC ACACGTTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG 
ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT 
ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG 
AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT 
GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG 
AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC 
ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA 
CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT 
GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA 
GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA 
TTTGCAGCCT TGCTGATTAA GTGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC 
ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCGG AGAATCTGGC TGCGGCACAG 
AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC 
CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA 
AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG 
AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT 
CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA 
TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC 
AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGCGAT 
GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT 
GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT 
TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA 
CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_012965258.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820712.1 Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

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CloneID	OXa35256
Clone ID Related Accession (Same CDS sequence)	XM_012965258.2
Accession Version	XM_012965258.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC 
CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG 
CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA 
CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG 
TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT 
GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG 
TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA 
ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT 
GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC 
AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA 
GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG 
GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT 
CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA 
GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT 
GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG 
CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA 
ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT 
TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC 
AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC 
TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA 
ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG 
CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG 
GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT 
AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA 
ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA 
TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA 
CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC 
ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC 
CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC 
CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA 
GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT 
GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT 
GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT 
GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA 
GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA 
GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA 
AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT 
GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA 
TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC 
ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT 
AAGGTCGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA 
CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT 
TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG 
GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG 
AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT 
GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA 
ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC 
CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT 
AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT 
GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC 
AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG 
CACACGTTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT 
AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT 
CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG 
CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT 
GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC 
TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT 
CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC 
GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT 
CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA 
GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC 
TTGCTGATTA AGTGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC 
TTCCCTGCAA CCAGTAGAAA AGAAGATGCG GAGAATCTGG CTGCGGCACA GAGGGACACG 
GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC 
CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT 
AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA 
CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT 
ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT 
GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC 
TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGCGA TGAACGGTTC 
AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC 
CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC 
TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC 
CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820712.1
CDS	331..5901
Translation	MFLTRALDKILSDKEVKRAHHSQLRKACEVALEEIKCETEKQSSPQDESKSGSSTLPPVKSKATFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEDVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLVNQTTAKATLTQMLNVIFARMENQALQEAKHMEKERHKQQHHLQHSPVSHHELELPPMRHHLEQTDQLTREHEGAIDQHLNYMENSLQEEAEPENGYDAPSAENELLETNQCAENHSKADLEIGDETSIDCEESTQEVIQSIIQEMLNSVVGEEETVRAECTARISEDPSDGDSIHANGIPGTPISMAYIPSLPDDRLSVSSNDTQESGNTSAPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPVFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKLHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDLYVNPNSQTTLGQEKPSEQDTHDTKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTSPDDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFQGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKSKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELAITTKSSKPSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFCIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGSSWHEILKCISQLELAQLIGTGVKPRYISGTVRNREGSFTGTKDQASDEFVSLGLVGGNVDWKQMASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAVVDFVRWLCAVSMDELLSPMHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTFEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCMLDIFKTTIPHALLTWKPAGGDTELSSQADGRDKQADSVSQKSVDIHESIQPRTADSRRNIQTQVSLVAQEEGHLARPGARVAEQRLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDTVHLDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTSLWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDDSRSDAWDEVQHRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIIQFDLIPELRAILRRFFHRIGLVFKISIPQEQEPQRVEEKLEAPDSQYPEGRRKETVQPESF

Target ORF information:

RefSeq Version	XM_012965258.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965258.2

ORF Insert Sequence:

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ATGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAATG AGCTTCTTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACATCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC 
CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG 
CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA 
CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG 
TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT 
GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG 
TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA 
ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT 
GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC 
AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA 
GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG 
GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT 
CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA 
GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT 
GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG 
CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA 
ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT 
TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC 
AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC 
TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA 
ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG 
CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG 
GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT 
AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA 
ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA 
TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA 
CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC 
ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC 
CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC 
CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA 
GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT 
GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT 
GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT 
GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA 
GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA 
GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA 
AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT 
GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA 
TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC 
ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT 
AAGGTCGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA 
CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT 
TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG 
GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG 
AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT 
GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA 
ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC 
CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT 
AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT 
GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC 
AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG 
CACACGTTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT 
AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT 
CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG 
CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT 
GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC 
TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT 
CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC 
GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT 
CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA 
GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC 
TTGCTGATTA AGTGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC 
TTCCCTGCAA CCAGTAGAAA AGAAGATGCG GAGAATCTGG CTGCGGCACA GAGGGACACG 
GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC 
CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT 
AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA 
CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT 
ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT 
GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC 
TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGCGA TGAACGGTTC 
AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC 
CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC 
TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC 
CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_012965257.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820711.2 Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

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CloneID	OXa60863
Clone ID Related Accession (Same CDS sequence)	XM_012965257.3
Accession Version	XM_012965257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT 
AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG 
TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG 
GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT 
AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA 
GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA 
AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC 
ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA 
AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG 
AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA 
GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA 
GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT 
CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC 
AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC 
TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG 
GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA 
AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT 
GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA 
GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA 
GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA 
GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA 
GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG 
TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC 
AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC 
TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT 
ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA 
GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG 
GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG 
ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA 
TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG 
CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT 
GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC 
CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG 
CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT 
CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT 
CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA 
GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA 
AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT 
GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG 
TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC 
CACTTTAATA AGGTTGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT 
CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG 
AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA 
GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA 
GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA 
GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC 
CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT 
GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT 
GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA 
GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC 
AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA 
ACCTATGGGC ACACATTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG 
ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT 
ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG 
AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT 
GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG 
AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC 
ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA 
CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT 
GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA 
GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA 
TTTGCAGCCT TGCTGATTAA ATGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC 
ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCAG AGAATCTGGC TGCGGCACAG 
AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC 
CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA 
AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG 
AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT 
CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA 
TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC 
AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGTGAT 
GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT 
GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT 
TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA 
CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820711.2
CDS	297..5876
Translation	MFLTRALDKILSDKEVKRAHHSQLRKACEVALEEIKCETEKQSSPQDESKSGSSTLPPVKSKATFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEDVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLVNQTTAKATLTQMLNVIFARMENQALQEAKHMEKERHKQQHHLQHSPVSHHELELPPMRHHLEQTDQLTREHEGAIDQHLNYMENSLQEEAEPENGYDAPSAENELPETNQCAENHSKADLEIGDETSIDCEESTQEVIQSIIQEMLNSVVGEEETVRAECTARISEDPSDGDSIHANGIPGTPISMAYTPSLPDDRLSVSSNDTQARKESGNTSAPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPVFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKLHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDLYVNPNSQTTLGQEKPSEQDTHDTKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTSPDDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFQGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKSKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELAITTKSSKPSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFCIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGSSWHEILKCISQLELAQLIGTGVKPRYISGTVRNREGSFTGTKDQASDEFVSLGLVGGNVDWKQMASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAVVDFVRWLCAVSMDELLSPMHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTFEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCMLDIFKTTIPHALLTWKPAGGDTELSSQADGRDKQADSVSQKSVDIHESIQPRTADSRRNIQTQVSLVAQEEGHLARPGARVAEQRLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDTVHLDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTSLWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDDSRSDAWDEVQHRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIIQFDLIPELRAILRRFFHRIGLVFKISIPQEQEPQRVEEKLEAPDSQYPEGRRKETVQPESF

Target ORF information:

RefSeq Version	XM_012965257.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965257.3

ORF Insert Sequence:

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGCAAG AAAGGAATCT GGAAACACTT CGGCACCTAC TCCTGGTGCT 
AAGTTCTCCC ATATCTTACA AAAAGATGCC TTTCTAGTCT TCAGGTCACT ATGTAAATTG 
TCTATGAAGC CACTGTCTGA TGGGCCACCA GACCCAAAAT CTCATGAATT GCGCTCCAAG 
GTACTTTCAC TCCAGCTGCT ACTCTCCATT CTGCAGAACG CAGGGCCTGT TTTTAAGACT 
AATGAAATGT TTATCAATGC AATCAAGCAG TATCTCTGTG TTGCTCTGTC TAAAAATGGA 
GTCTCGTCTG TTCCAGAGGT CTTTGAGCTG TCTCTGTCTA TTTTTTTGAC ACTGTTATCA 
AATTTCAAGT TACATCTGAA AATGCAGATT GAGGTTTTCT TTAAGGAAAT CTTCCTTTAC 
ATACTGGAAA CATCTACTAG CTCATTTGAT CACAAGTGGA TGGTGATTCA GACACTGACA 
AGGATTTGTG CAGATGCACA AAGTGTGGTT GATATTTATG TCAATTATGA CTGTGATTTG 
AATGCAGCCA ACATCTTTGA AAGGTTGGTT AATGACCTCT CCAAGATTGC CCAGGGACGA 
GGAAGCCAAG AACTTGGCAT GTCAAATGTT CAGGAGTTAA GCTTACGGAA GAAAGGCTTA 
GAATGCCTGG TTTCCATTTT GAAATGTATG GTGGAATGGA GTAAAGATCT CTATGTCAAT 
CCCAACTCTC AGACAACTCT AGGGCAAGAG AAGCCTTCTG AGCAAGATAC CCATGACACC 
AAACACCCAG AGACCATCAA TAGATATGGA AGCTTGAATT CACTTGATTC TACGGCTTCC 
TCAGGCATTG GGAGCTACAG CACACAAATG TCAGGAACAG ACAACCCAGA GCAGTTTGAG 
GTTCTGAAGC AACAAAAAGA GATCATAGAG CAAGGCATAG ATCTCTTTAA TAAGAAACCA 
AAGAGAGGAA TTCAGTATCT GCAAGAACAA GGAATGCTTG GAACATCGCC AGACGATATT 
GCTCAGTTTT TGCACCAAGA AGAGAGATTA GACTCTACTC AAGTTGGGGA GTTCCTTGGA 
GATAATGACA AGTTCAACAA AGAAGTTATG TATGCTTATG TTGACCAGCA TGACTTCCAA 
GGAAAAGACT TTGTATCGGC CCTTCGGATG TTTCTGGAAG GTTTCCGTCT TCCAGGGGAA 
GCCCAAAAAA TTGATCGTTT AATGGAAAAG TTTGCTGCTC GATATCTTGA ATGTAACCAA 
GGGCAGACGC TGTTTGCTAG TGCAGATACT GCTTATGTCT TGGCGTATTC AATTATCATG 
TTGACCACGG ACCTTCACAG CCCTCAGGTG AAGAGTAAAA TGACGAAAGA ACAGTATATC 
AAAATGAATA GGGGTATCAA CGATAGCAAG GATCTTCCTG AAGAGTATCT GTCTGCTATC 
TACAATGAAA TAGCTGGAAA GAAAATCTCC ATGAAAGAAA CAAAAGAACT TGCCATCACT 
ACCAAGTCAT CAAAGCCAAG TGTTGCTAGT GAAAAACAAA GAAGACTGTT GTACAATTTA 
GAAATGGAAC AAATGGCCAA GACGGCAAAA GCCCTTATGG AGGCTGTAAG TCATGTCCAG 
GCTCCCTTCA CCAGTGCTAC TCATTTAGAA CATGTCAGGC CAATGTTCAA GCTGGCATGG 
ACACCATTCC TGGCTGCATT CAGCGTCGGC CTGCAAGACT GTGATGACAC TGAAGTAGCA 
TCTCTGTGCC TGGAGGGCAT TCGTTGTGCC ATTAGGATTG CATGCATCTT CTGTATACAG 
CTGGAGAGAG ATGCCTACGT TCAAGCCCTA GCGAGGTTTA CATTATTAAC TGTGAGCTCT 
GGAATTACTG AAATGAAACA GAAGAACATA GATACTATCA AAACTCTCAT TACAGTGGCC 
CATACTGATG GAAACTACTT GGGGTCGTCT TGGCATGAGA TTTTGAAGTG TATTAGTCAG 
CTGGAGCTTG CTCAATTGAT TGGCACCGGG GTGAAGCCAC GTTACATATC TGGAACTGTT 
CGAAACAGAG AAGGATCTTT TACTGGCACT AAAGACCAAG CATCTGATGA GTTTGTCAGT 
CTTGGGTTAG TTGGGGGAAA TGTGGACTGG AAACAAATGG CAAGCATTCA GGAATCCATA 
GGGGAGACAA GTTCACAAAG CGTTGTGGTG GCTGTTGACA GGATATTTAC AGGATCCACA 
AGACTGGATG GTAATGCAGT AGTGGATTTT GTGCGTTGGC TATGTGCAGT CTCCATGGAT 
GAATTGTTAT CTCCGATGCA CCCTCGTATG TTCAGCTTAC AGAAGATTGT AGAGATATCG 
TACTACAACA TGGGCCGCAT CAGGCTGCAG TGGTCTAGGA TATGGGAAGT CATTGGAGAC 
CACTTTAATA AGGTTGGCTG CAATCCAAAT GAAGATGTGG CGATCTTTGC AGTGGACTCT 
CTAAGGCAAC TCTCAATGAA ATTTTTAGAA AAGGGAGAGC TTGCAAACTT CCGTTTCCAG 
AAGGACTTTT TAAGACCCTT TGAGCACATC ATGAAAAGAA ATCGGTCTCC TACCATTAGA 
GATATGGTGG TACGCTGCAT TGCACAAATG GTGAATTCTC AAGCTGCTAA CATTCGCTCA 
GGCTGGAAGA ACATCTTCTC TGTCTTTCAT TTAGCTGCGT CAGATCAGGA TGAAAGCATA 
GTGGAGCTTG CATTTCAGAC CACTGGGCAC ATAGTCACAC TTGTGTTTGA GAAACACTTC 
CCTGCCACAA TTGATTCCTT CCAAGATGCA GTTAAGTGTT TGTCTGAATT TGCGTGTAAT 
GCTGCTTTCC CGGACACAAG CATGGAAGCC ATTCGTCTGA TTCGTCATTG TGCAAAATAT 
GTTTCAGATA GGCCACAGGC CTTCAAGGAG TATACTAGTG ATGATATGAA CGTAGCTCCA 
GAAGACCGTG TGTGGGTGAG AGGATGGTTT CCCATCCTGT TTGAGCTGTC CTGCATCATC 
AACAGATGCA AATTAGATGT AAGAACCAGA GGTCTGACTG TCATGTTTGA GGTGATGAAA 
ACCTATGGGC ACACATTTGA GAAACACTGG TGGCAAGATT TATTCAGGAT TGTATTCAGG 
ATATTTGATA ACATGAAACT TCCAGAGCAA CAGACTGAGA AGGCAGAATG GATGACTACT 
ACATGCAATC ATGCCCTCTA TGCAATTTGT GATGTGTTTA CACAGTACTT TGAGGTACTG 
AGCGATGTGC TGCTGGATGA TATCTTTGCA CAGCTCTACT GGTGTGTGCA GCAAGATAAT 
GAACAGCTTG CTCGATCTGG CACCAACTGC TTAGAGAATG TTGTTATTCT GAATGGAGAG 
AAATTTACCT TGGAAATCTG GGATAAAACC TGCAACTGTA TGCTGGATAT TTTTAAAACC 
ACAATCCCTC ACGCGTTACT GACATGGAAA CCTGCAGGAG GAGACACAGA ACTGAGCTCA 
CAGGCTGACG GCAGGGACAA GCAAGCGGAT TCAGTGTCCC AGAAATCTGT AGATATCCAT 
GAATCTATTC AGCCTCGCAC AGCCGACAGC AGGAGAAACA TCCAGACTCA AGTTTCCCTA 
GTGGCCCAAG AGGAGGGGCA TTTAGCAAGG CCTGGTGCAA GAGTGGCAGA GCAGCGCTTA 
TTTGCAGCCT TGCTGATTAA ATGCGTGGTG CAGCTGGAGC TCATACAGAC CATTGACAAC 
ATTGTGTTCT TCCCTGCAAC CAGTAGAAAA GAAGATGCAG AGAATCTGGC TGCGGCACAG 
AGGGACACGG TGCATTTAGA TGTTCATGTG GACACTCAGG ATCAAGGCAT GTATCGCTTC 
CTCACATCCC AACAGCTCTT CAAACTCCTG GATTGTCTGC TGGAGTCTCA CCGATTTGCA 
AAGGCCTTTA ATTCCAATAA CGAGCAAAGG ACTTCTCTGT GGAAGGCAGG CTTCAAAGGG 
AAATCGAAAC CCAATCTCCT GAAACAAGAG ACCAGCAGCC TGGCCTGTGG CTTACGCATT 
CTGTTCCGTA TGTACATGGA CGACAGCCGG AGCGATGCGT GGGATGAAGT TCAGCATCGA 
TTGCTTAATG TTTGCAGTGA GGCCCTGAGT TACTTTCTGA CCCTGACATC CGAGAGTCAC 
AGAGAGGCCT GGACTAATCT GCTGCTGCTC TTCTTAACCA AAGTGCTGAA GATTAGTGAT 
GAACGGTTCA AAGCTCACGC ATCATTCTAT TATCCTCTGC TCTGCGAGAT AATCCAGTTT 
GACCTCATCC CGGAGTTGCG CGCTATCCTG CGGCGGTTCT TCCACCGGAT CGGCCTTGTT 
TTTAAAATCT CCATCCCACA GGAGCAGGAG CCCCAAAGAG TAGAAGAAAA GTTGGAAGCA 
CCTGACTCCC AGTATCCAGA AGGGAGGAGG AAGGAGACCG TCCAACCAGA AAGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arfgef1 ( XM_012965258.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820712.2 Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

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CloneID	OXa60864
Clone ID Related Accession (Same CDS sequence)	XM_012965258.3
Accession Version	XM_012965258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC 
CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG 
CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA 
CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG 
TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT 
GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG 
TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA 
ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT 
GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC 
AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA 
GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG 
GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT 
CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA 
GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT 
GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG 
CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA 
ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT 
TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC 
AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC 
TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA 
ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG 
CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG 
GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT 
AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA 
ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA 
TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA 
CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC 
ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC 
CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC 
CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA 
GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT 
GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT 
GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT 
GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA 
GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA 
GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA 
AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT 
GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA 
TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC 
ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT 
AAGGTTGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA 
CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT 
TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG 
GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG 
AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT 
GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA 
ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC 
CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT 
AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT 
GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC 
AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG 
CACACATTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT 
AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT 
CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG 
CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT 
GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC 
TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT 
CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC 
GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT 
CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA 
GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC 
TTGCTGATTA AATGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC 
TTCCCTGCAA CCAGTAGAAA AGAAGATGCA GAGAATCTGG CTGCGGCACA GAGGGACACG 
GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC 
CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT 
AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA 
CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT 
ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT 
GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC 
TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGTGA TGAACGGTTC 
AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC 
CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC 
TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC 
CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820712.2
CDS	296..5866
Translation	MFLTRALDKILSDKEVKRAHHSQLRKACEVALEEIKCETEKQSSPQDESKSGSSTLPPVKSKATFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEDVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLVNQTTAKATLTQMLNVIFARMENQALQEAKHMEKERHKQQHHLQHSPVSHHELELPPMRHHLEQTDQLTREHEGAIDQHLNYMENSLQEEAEPENGYDAPSAENELPETNQCAENHSKADLEIGDETSIDCEESTQEVIQSIIQEMLNSVVGEEETVRAECTARISEDPSDGDSIHANGIPGTPISMAYTPSLPDDRLSVSSNDTQESGNTSAPTPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKVLSLQLLLSILQNAGPVFKTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKLHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDLYVNPNSQTTLGQEKPSEQDTHDTKHPETINRYGSLNSLDSTASSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTSPDDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFQGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKSKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELAITTKSSKPSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFCIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGSSWHEILKCISQLELAQLIGTGVKPRYISGTVRNREGSFTGTKDQASDEFVSLGLVGGNVDWKQMASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAVVDFVRWLCAVSMDELLSPMHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEVMKTYGHTFEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYFEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCMLDIFKTTIPHALLTWKPAGGDTELSSQADGRDKQADSVSQKSVDIHESIQPRTADSRRNIQTQVSLVAQEEGHLARPGARVAEQRLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDTVHLDVHVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTSLWKAGFKGKSKPNLLKQETSSLACGLRILFRMYMDDSRSDAWDEVQHRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDERFKAHASFYYPLLCEIIQFDLIPELRAILRRFFHRIGLVFKISIPQEQEPQRVEEKLEAPDSQYPEGRRKETVQPESF

Target ORF information:

RefSeq Version	XM_012965258.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADP ribosylation factor guanine nucleotide exchange factor 1 (arfgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965258.3

ORF Insert Sequence:

ATGTTTCTCA CCAGAGCTCT GGATAAGATC CTTAGCGATA AGGAGGTGAA GCGAGCGCAC 
CATAGCCAGC TGCGGAAAGC CTGCGAGGTG GCGCTAGAGG AAATAAAATG TGAAACAGAA 
AAACAAAGTT CACCTCAAGA TGAATCAAAA TCTGGATCTA GTACCCTTCC ACCTGTGAAA 
TCAAAGGCCA CTTTTATAGA AGCTGACAAG TACTTTTTAC CCTTCGAGTT AGCGTGCCAG 
TCCAAATGTC CGCGAATTGT CAGTACATCT TTGGACTGCC TGCAGAAGCT TATAGCTTAT 
GGACACTTGA CTGGAAATGC GCCAGACAGC ACAACTCCTG GGAAAAAATT GATCGACAGA 
ATAATCGAAA CCATATGTGG CTGTTTTCAA GGACCCCAAA CAGATGAAGA TGTTCAGTTA 
CAAATAATTA AGGCACTACT CACCGCTGTT ACATCGCAAC ACATTGAAAT ACACGAAGGC 
ACAGTGCTGC AAGCTGTAAG AACATGCTAC AACATCTATC TAGCCAGCAA AAACCTTGTC 
AATCAGACTA CAGCCAAGGC CACCTTAACA CAAATGCTCA ACGTAATATT TGCACGAATG 
GAAAACCAAG CCCTTCAGGA AGCCAAACAC ATGGAAAAGG AGCGGCATAA ACAGCAACAC 
CATTTGCAGC ACTCACCAGT AAGCCACCAT GAGCTGGAAT TGCCACCCAT GAGGCACCAT 
CTAGAGCAAA CTGACCAGTT AACTAGAGAA CATGAAGGGG CTATTGACCA ACACTTAAAC 
TACATGGAAA ATAGCCTGCA GGAAGAAGCT GAGCCAGAAA ATGGATATGA TGCTCCAAGT 
GCAGAAAACG AGCTTCCTGA AACCAATCAG TGTGCAGAAA ATCATTCCAA AGCTGATTTG 
GAAATTGGGG ATGAAACCAG TATTGACTGT GAAGAATCTA CACAGGAAGT AATCCAAAGC 
ATTATTCAAG AAATGCTGAA CTCTGTAGTG GGAGAGGAAG AGACTGTCAG GGCCGAATGT 
ACAGCAAGGA TATCAGAAGA TCCCAGTGAT GGGGATAGTA TACATGCCAA TGGTATACCT 
GGAACTCCCA TATCTATGGC CTACACCCCA TCCTTACCCG ATGATAGATT GTCTGTTTCC 
TCTAACGACA CTCAGGAATC TGGAAACACT TCGGCACCTA CTCCTGGTGC TAAGTTCTCC 
CATATCTTAC AAAAAGATGC CTTTCTAGTC TTCAGGTCAC TATGTAAATT GTCTATGAAG 
CCACTGTCTG ATGGGCCACC AGACCCAAAA TCTCATGAAT TGCGCTCCAA GGTACTTTCA 
CTCCAGCTGC TACTCTCCAT TCTGCAGAAC GCAGGGCCTG TTTTTAAGAC TAATGAAATG 
TTTATCAATG CAATCAAGCA GTATCTCTGT GTTGCTCTGT CTAAAAATGG AGTCTCGTCT 
GTTCCAGAGG TCTTTGAGCT GTCTCTGTCT ATTTTTTTGA CACTGTTATC AAATTTCAAG 
TTACATCTGA AAATGCAGAT TGAGGTTTTC TTTAAGGAAA TCTTCCTTTA CATACTGGAA 
ACATCTACTA GCTCATTTGA TCACAAGTGG ATGGTGATTC AGACACTGAC AAGGATTTGT 
GCAGATGCAC AAAGTGTGGT TGATATTTAT GTCAATTATG ACTGTGATTT GAATGCAGCC 
AACATCTTTG AAAGGTTGGT TAATGACCTC TCCAAGATTG CCCAGGGACG AGGAAGCCAA 
GAACTTGGCA TGTCAAATGT TCAGGAGTTA AGCTTACGGA AGAAAGGCTT AGAATGCCTG 
GTTTCCATTT TGAAATGTAT GGTGGAATGG AGTAAAGATC TCTATGTCAA TCCCAACTCT 
CAGACAACTC TAGGGCAAGA GAAGCCTTCT GAGCAAGATA CCCATGACAC CAAACACCCA 
GAGACCATCA ATAGATATGG AAGCTTGAAT TCACTTGATT CTACGGCTTC CTCAGGCATT 
GGGAGCTACA GCACACAAAT GTCAGGAACA GACAACCCAG AGCAGTTTGA GGTTCTGAAG 
CAACAAAAAG AGATCATAGA GCAAGGCATA GATCTCTTTA ATAAGAAACC AAAGAGAGGA 
ATTCAGTATC TGCAAGAACA AGGAATGCTT GGAACATCGC CAGACGATAT TGCTCAGTTT 
TTGCACCAAG AAGAGAGATT AGACTCTACT CAAGTTGGGG AGTTCCTTGG AGATAATGAC 
AAGTTCAACA AAGAAGTTAT GTATGCTTAT GTTGACCAGC ATGACTTCCA AGGAAAAGAC 
TTTGTATCGG CCCTTCGGAT GTTTCTGGAA GGTTTCCGTC TTCCAGGGGA AGCCCAAAAA 
ATTGATCGTT TAATGGAAAA GTTTGCTGCT CGATATCTTG AATGTAACCA AGGGCAGACG 
CTGTTTGCTA GTGCAGATAC TGCTTATGTC TTGGCGTATT CAATTATCAT GTTGACCACG 
GACCTTCACA GCCCTCAGGT GAAGAGTAAA ATGACGAAAG AACAGTATAT CAAAATGAAT 
AGGGGTATCA ACGATAGCAA GGATCTTCCT GAAGAGTATC TGTCTGCTAT CTACAATGAA 
ATAGCTGGAA AGAAAATCTC CATGAAAGAA ACAAAAGAAC TTGCCATCAC TACCAAGTCA 
TCAAAGCCAA GTGTTGCTAG TGAAAAACAA AGAAGACTGT TGTACAATTT AGAAATGGAA 
CAAATGGCCA AGACGGCAAA AGCCCTTATG GAGGCTGTAA GTCATGTCCA GGCTCCCTTC 
ACCAGTGCTA CTCATTTAGA ACATGTCAGG CCAATGTTCA AGCTGGCATG GACACCATTC 
CTGGCTGCAT TCAGCGTCGG CCTGCAAGAC TGTGATGACA CTGAAGTAGC ATCTCTGTGC 
CTGGAGGGCA TTCGTTGTGC CATTAGGATT GCATGCATCT TCTGTATACA GCTGGAGAGA 
GATGCCTACG TTCAAGCCCT AGCGAGGTTT ACATTATTAA CTGTGAGCTC TGGAATTACT 
GAAATGAAAC AGAAGAACAT AGATACTATC AAAACTCTCA TTACAGTGGC CCATACTGAT 
GGAAACTACT TGGGGTCGTC TTGGCATGAG ATTTTGAAGT GTATTAGTCA GCTGGAGCTT 
GCTCAATTGA TTGGCACCGG GGTGAAGCCA CGTTACATAT CTGGAACTGT TCGAAACAGA 
GAAGGATCTT TTACTGGCAC TAAAGACCAA GCATCTGATG AGTTTGTCAG TCTTGGGTTA 
GTTGGGGGAA ATGTGGACTG GAAACAAATG GCAAGCATTC AGGAATCCAT AGGGGAGACA 
AGTTCACAAA GCGTTGTGGT GGCTGTTGAC AGGATATTTA CAGGATCCAC AAGACTGGAT 
GGTAATGCAG TAGTGGATTT TGTGCGTTGG CTATGTGCAG TCTCCATGGA TGAATTGTTA 
TCTCCGATGC ACCCTCGTAT GTTCAGCTTA CAGAAGATTG TAGAGATATC GTACTACAAC 
ATGGGCCGCA TCAGGCTGCA GTGGTCTAGG ATATGGGAAG TCATTGGAGA CCACTTTAAT 
AAGGTTGGCT GCAATCCAAA TGAAGATGTG GCGATCTTTG CAGTGGACTC TCTAAGGCAA 
CTCTCAATGA AATTTTTAGA AAAGGGAGAG CTTGCAAACT TCCGTTTCCA GAAGGACTTT 
TTAAGACCCT TTGAGCACAT CATGAAAAGA AATCGGTCTC CTACCATTAG AGATATGGTG 
GTACGCTGCA TTGCACAAAT GGTGAATTCT CAAGCTGCTA ACATTCGCTC AGGCTGGAAG 
AACATCTTCT CTGTCTTTCA TTTAGCTGCG TCAGATCAGG ATGAAAGCAT AGTGGAGCTT 
GCATTTCAGA CCACTGGGCA CATAGTCACA CTTGTGTTTG AGAAACACTT CCCTGCCACA 
ATTGATTCCT TCCAAGATGC AGTTAAGTGT TTGTCTGAAT TTGCGTGTAA TGCTGCTTTC 
CCGGACACAA GCATGGAAGC CATTCGTCTG ATTCGTCATT GTGCAAAATA TGTTTCAGAT 
AGGCCACAGG CCTTCAAGGA GTATACTAGT GATGATATGA ACGTAGCTCC AGAAGACCGT 
GTGTGGGTGA GAGGATGGTT TCCCATCCTG TTTGAGCTGT CCTGCATCAT CAACAGATGC 
AAATTAGATG TAAGAACCAG AGGTCTGACT GTCATGTTTG AGGTGATGAA AACCTATGGG 
CACACATTTG AGAAACACTG GTGGCAAGAT TTATTCAGGA TTGTATTCAG GATATTTGAT 
AACATGAAAC TTCCAGAGCA ACAGACTGAG AAGGCAGAAT GGATGACTAC TACATGCAAT 
CATGCCCTCT ATGCAATTTG TGATGTGTTT ACACAGTACT TTGAGGTACT GAGCGATGTG 
CTGCTGGATG ATATCTTTGC ACAGCTCTAC TGGTGTGTGC AGCAAGATAA TGAACAGCTT 
GCTCGATCTG GCACCAACTG CTTAGAGAAT GTTGTTATTC TGAATGGAGA GAAATTTACC 
TTGGAAATCT GGGATAAAAC CTGCAACTGT ATGCTGGATA TTTTTAAAAC CACAATCCCT 
CACGCGTTAC TGACATGGAA ACCTGCAGGA GGAGACACAG AACTGAGCTC ACAGGCTGAC 
GGCAGGGACA AGCAAGCGGA TTCAGTGTCC CAGAAATCTG TAGATATCCA TGAATCTATT 
CAGCCTCGCA CAGCCGACAG CAGGAGAAAC ATCCAGACTC AAGTTTCCCT AGTGGCCCAA 
GAGGAGGGGC ATTTAGCAAG GCCTGGTGCA AGAGTGGCAG AGCAGCGCTT ATTTGCAGCC 
TTGCTGATTA AATGCGTGGT GCAGCTGGAG CTCATACAGA CCATTGACAA CATTGTGTTC 
TTCCCTGCAA CCAGTAGAAA AGAAGATGCA GAGAATCTGG CTGCGGCACA GAGGGACACG 
GTGCATTTAG ATGTTCATGT GGACACTCAG GATCAAGGCA TGTATCGCTT CCTCACATCC 
CAACAGCTCT TCAAACTCCT GGATTGTCTG CTGGAGTCTC ACCGATTTGC AAAGGCCTTT 
AATTCCAATA ACGAGCAAAG GACTTCTCTG TGGAAGGCAG GCTTCAAAGG GAAATCGAAA 
CCCAATCTCC TGAAACAAGA GACCAGCAGC CTGGCCTGTG GCTTACGCAT TCTGTTCCGT 
ATGTACATGG ACGACAGCCG GAGCGATGCG TGGGATGAAG TTCAGCATCG ATTGCTTAAT 
GTTTGCAGTG AGGCCCTGAG TTACTTTCTG ACCCTGACAT CCGAGAGTCA CAGAGAGGCC 
TGGACTAATC TGCTGCTGCT CTTCTTAACC AAAGTGCTGA AGATTAGTGA TGAACGGTTC 
AAAGCTCACG CATCATTCTA TTATCCTCTG CTCTGCGAGA TAATCCAGTT TGACCTCATC 
CCGGAGTTGC GCGCTATCCT GCGGCGGTTC TTCCACCGGA TCGGCCTTGT TTTTAAAATC 
TCCATCCCAC AGGAGCAGGA GCCCCAAAGA GTAGAAGAAA AGTTGGAAGC ACCTGACTCC 
CAGTATCCAG AAGGGAGGAG GAAGGAGACC GTCCAACCAG AAAGCTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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arfgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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