Itga4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itga4
Entrez Gene ID	311144
Full Name	integrin subunit alpha 4
General protein information	Preferred Names integrin subunit alpha 4 Names integrin alpha-4 integrin, alpha 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24
Summary	mouse homolog is involved in adult hematopoietic cell homeostasis, regeneration, and homing [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591704.1	XP_017447193.1	integrin alpha-4 isoform X1
XM_017591705.1	XP_017447194.1	integrin alpha-4 isoform X2
NM_001107737.1	NP_001101207.1	integrin alpha-4
XM_017591706.1	XP_017447195.1	integrin alpha-4 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	itga4	XP_002663459.2
Xenopus tropicalis (tropical clawed frog)	itga4	XP_002932062.2
Rattus norvegicus (Norway rat)	Itga4	NP_001101207.1
Homo sapiens (human)	ITGA4	NP_000876.3
Macaca mulatta (Rhesus monkey)	ITGA4	XP_001100929.1
Canis lupus familiaris (dog)	ITGA4	XP_545551.3
Pan troglodytes (chimpanzee)	ITGA4	XP_525977.2
Bos taurus (cattle)	ITGA4	NP_777173.1
Mus musculus (house mouse)	Itga4	NP_034706.3
Gallus gallus (chicken)	ITGA4	XP_421974.3

Gene Ontology
Bibliography

Related articles in PubMed

MiR-30s Family Inhibit the Proliferation and Apoptosis in Human Coronary Artery Endothelial Cells Through Targeting the 3'UTR Region of ITGA4 and PLCG1.
Ma F, Li T, Zhang H, Wu G
Journal of cardiovascular pharmacology68(5)327-333(2016 Nov)

Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of α₄β₇.
Zhang K, Pan Y, Qi J, Yue J, Zhang M, Xu C, Li G, Chen J
Journal of cell science126(Pt 21)5030-41(2013 Nov)

GPR15-mediated homing controls immune homeostasis in the large intestine mucosa.
Kim SV, Xiang WV, Kwak C, Yang Y, Lin XW, Ota M, Sarpel U, Rifkin DB, Xu R, Littman DR
Science (New York, N.Y.)340(6139)1456-9(2013 Jun)

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

A positive feedback loop involving Gcm1 and Fzd5 directs chorionic branching morphogenesis in the placenta.
Lu J, Zhang S, Nakano H, Simmons DG, Wang S, Kong S, Wang Q, Shen L, Tu Z, Wang W, Wang B, Wang H, Wang Y, van Es JH, Clevers H, Leone G, Cross JC, Wang H
PLoS biology11(4)e1001536(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Itga4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itga4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60385	XM_017591705.1 Latest version!	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa60386	XM_017591706.1 Latest version!	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa05793	NM_001107737.1 Latest version!	Rattus norvegicus integrin subunit alpha 4 (Itga4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa60384	XM_017591704.1 Latest version!	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itga4 ( XM_017591705.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447194.1 Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60385
Clone ID Related Accession (Same CDS sequence)	XM_017591705.1
Accession Version	XM_017591705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	integrin alpha-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACA ATAATAAACT CCCCACTGGT GTTTGCTATG GCATGCCTTC TGATTTGCGG 
ACAGAACTAA GTAAAAGGAT GGCCCCCTGT TACCAAGATT TTGTGAGAAA ATTTGGAGAA 
AATTTTGCAT CGTGTCAAGC TGGAATATCT AGTTTTTACA CACAGGATTT GATTGTGATG 
GGGGCCCCGG GATCATCGTA CTGGACCGGC TCCGTCTTTG TCTACAACAT AACTACGAAC 
AAATACAAGG CATTTGTAGA CAGACAGAAT CAAGTAAAAT TTGGAAGCTA CTTAGGCTAC 
TCAGTAGGAG CTGGTCATTT CCGAAATCCA CATACTACCG AAGTCGTGGG AGGAGCCCCT 
CAACACGAAC AGATAGGAAA AGCATATATA TTCAGCATTG ATGAAAGCGA ACTGACCATC 
GTATATGAAA TGAAAGGTAA AAAGCTTGGC TCTTACTTTG GAGCTTCTGT CTGTGCGGTG 
GACCTCAATG CAGATGGCTT CTCAGATCTC CTTGTTGGAG CGCCTATGCA GAGCACCATC 
AGGGAGGAAG GAAGAGTGTT CGTGTACATC AACTCTGGCA TGGGAGCTGT GATGGTTGAA 
ATGGAAAGAG TGCTCGTCGG AAGTGACAAA TATGCTGCCA GGTTTGGGGA ATCCATGGCA 
AATCTTGGCG ACATTGACAA TGATGGCTTT GAAGATATTG CTATTGGCGC ACCACAAGAA 
GATGACTTGC GAGGCGCTGT CTACATTTAC AATGGCCGAG TAGATGGCAT CTCCTCTACA 
TACTCACAGA GAATCGAAGG ACGTCAAATC AGCCAGTCAC TAAGGATGTT TGGACAGTCT 
ATCTCAGGAC AAATTGATGC AGACAACAAT GGATATGTTG ATGTAGCGGT TGGTGCATTT 
CAGTCTGATT CTGCAGTGTT GCTAAGGACA CGGCCTGTAG TGATTGTTGA AGCATCTTTA 
AGCCATCCTG AGTCAGTAAA TAGAACAAAA TTTGACTGTA CTGAAAATGG ACTTCTGTCT 
GTGTGCGTGG ATCTTAAACT GTGTTTCTCA TATAAGGGCA AAGAGGTCCC AGGCTACATC 
GTTTTGTTTT ACAATGTGAG CTTGGATGTG CACAGGAAGG CCGAGTCTCC GTCAAGATTT 
TATTTCTTCT CCAATGGGAC TTCTGACATG ATTACAAGAA GCATACGAGT TTCAAGCAGT 
GGAGAGAAAT GTAGGACACA CCAGGCATTC ATGCGGAAAG ACGTGCGAGA CATCCTCACT 
CCCATTCAGG TTGAGGCCAC ATACCACCTT GGGCATCATG TGATCACCAA ACGAAACACA 
GAGGAATTCC CACCCCTCCA GCCGATCCTT CAGCAGAAGA AAGAAAAAGA CATTATTAGA 
AAAACGATAA ACTTCGCAAG GTTTTGTGCC TATGAAAATT GTTCTGCTGA TCTGCAGGTT 
TCTGCAAAAG TTGGGTTTTT GAAGCCATAT GAAAATAAAA CCTATCTTGC TGTTGGGAGC 
ATGAAGACCA TAATGCTAAA TGTGTCCTTG TTCAATGCTG GAGATGATGC TTATGAAACT 
ACTCTGAATG TCCAACTCCC CACAGGCCTT TATTTCATTA AGATCTTAGA CCTGGAAGAG 
AAACAAATAA ACTGCGACGT GACAGAGAGC TCAGGCGTAG TTAAGCTTGC CTGCAGCCTC 
GGTTACATAT ACGTGGATCG TCTCTCAAGG ATAGACATTA GCTTTCTCCT GGATGTGAGC 
TCACTCAGCA GGGCCCATGA GGACCTCAGC ATCAACGTGC ATGCCTCCTG TGAAAATGAG 
GGTAAATTGG ACCAAGTGAA AGACAACAGA GTGACCTTAG TGATACCTCT AAGGTATGAA 
GTTATGCTGG CTGTTCACGG GTTTGTGACC CCAACTTCGT TTGTGTATGG ATCCAGTGAA 
GAAAACGAGC CAGAAATGTG CATGGCAGAG AAATTGAACC TCACTTTCCA TGTTATAAAC 
ACTGGCATTA GCATGGCTCC AAATGTTAGT GTGAAAATAA TGGTACCAAA TTCTTTTCTC 
CCTCGAAATG ATAAGTTGTT CAACGTTTTG GATGTCCAGG CAACTATGGG GCAGTGCCAA 
TTTGAACACT ATGGACGAGA GTGTGCATTA GCACAGCAAA AAGGCATAGC GGGGACCTTG 
ACCGATTTGG TCAAATTTCT ATCAAAGACT GATAAGAGAC TCCTGTATTG CATGAAAGCT 
GATCGACACT GTTTAGATTT CTTATGTAAT TTCGGGAAAA TGGAAAGTGG GAAGGAAGCC 
AGCGTTCACA TTCAGCTGGA AGGCAGGCCA TCCATCTTGG AAATGGATGA GACCTCATCG 
CTCAAATTTG AAATAAAAGC AACAGCTTTT CCAGAGCCAC ACCCAAAAGT TATTGAGCTC 
AATAAGGATG AGAACATTGC CCATGTTTAC TTGGAAGGGC TCCATCATCA AAGACCCAAA 
CGGCATTTCA CCATCGTGAT TGTCACCATC AGCTTGCTCC TTGGACTTAT TGTACTTTTA 
TTAATTTCAT GTGTTATGTG GAAGGCTGGA TTTTTTAAAA GGCAGTACAA ATCTATCCTA 
CAAGAAGAAA ACAGGAGAGA CAGCTGGAGT TATGTCAACA GCAAAAGCAA TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447194.1
CDS	468..3107
Translation	MKNNNKLPTGVCYGMPSDLRTELSKRMAPCYQDFVRKFGENFASCQAGISSFYTQDLIVMGAPGSSYWTGSVFVYNITTNKYKAFVDRQNQVKFGSYLGYSVGAGHFRNPHTTEVVGGAPQHEQIGKAYIFSIDESELTIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGMGAVMVEMERVLVGSDKYAARFGESMANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQRIEGRQISQSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDCTENGLLSVCVDLKLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDMITRSIRVSSSGEKCRTHQAFMRKDVRDILTPIQVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDIIRKTINFARFCAYENCSADLQVSAKVGFLKPYENKTYLAVGSMKTIMLNVSLFNAGDDAYETTLNVQLPTGLYFIKILDLEEKQINCDVTESSGVVKLACSLGYIYVDRLSRIDISFLLDVSSLSRAHEDLSINVHASCENEGKLDQVKDNRVTLVIPLRYEVMLAVHGFVTPTSFVYGSSEENEPEMCMAEKLNLTFHVINTGISMAPNVSVKIMVPNSFLPRNDKLFNVLDVQATMGQCQFEHYGRECALAQQKGIAGTLTDLVKFLSKTDKRLLYCMKADRHCLDFLCNFGKMESGKEASVHIQLEGRPSILEMDETSSLKFEIKATAFPEPHPKVIELNKDENIAHVYLEGLHHQRPKRHFTIVIVTISLLLGLIVLLLISCVMWKAGFFKRQYKSILQEENRRDSWSYVNSKSNDD

Target ORF information:

RefSeq Version	XM_017591705.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591705.1

ORF Insert Sequence:

ATGAAGAACA ATAATAAACT CCCCACTGGT GTTTGCTATG GCATGCCTTC TGATTTGCGG 
ACAGAACTAA GTAAAAGGAT GGCCCCCTGT TACCAAGATT TTGTGAGAAA ATTTGGAGAA 
AATTTTGCAT CGTGTCAAGC TGGAATATCT AGTTTTTACA CACAGGATTT GATTGTGATG 
GGGGCCCCGG GATCATCGTA CTGGACCGGC TCCGTCTTTG TCTACAACAT AACTACGAAC 
AAATACAAGG CATTTGTAGA CAGACAGAAT CAAGTAAAAT TTGGAAGCTA CTTAGGCTAC 
TCAGTAGGAG CTGGTCATTT CCGAAATCCA CATACTACCG AAGTCGTGGG AGGAGCCCCT 
CAACACGAAC AGATAGGAAA AGCATATATA TTCAGCATTG ATGAAAGCGA ACTGACCATC 
GTATATGAAA TGAAAGGTAA AAAGCTTGGC TCTTACTTTG GAGCTTCTGT CTGTGCGGTG 
GACCTCAATG CAGATGGCTT CTCAGATCTC CTTGTTGGAG CGCCTATGCA GAGCACCATC 
AGGGAGGAAG GAAGAGTGTT CGTGTACATC AACTCTGGCA TGGGAGCTGT GATGGTTGAA 
ATGGAAAGAG TGCTCGTCGG AAGTGACAAA TATGCTGCCA GGTTTGGGGA ATCCATGGCA 
AATCTTGGCG ACATTGACAA TGATGGCTTT GAAGATATTG CTATTGGCGC ACCACAAGAA 
GATGACTTGC GAGGCGCTGT CTACATTTAC AATGGCCGAG TAGATGGCAT CTCCTCTACA 
TACTCACAGA GAATCGAAGG ACGTCAAATC AGCCAGTCAC TAAGGATGTT TGGACAGTCT 
ATCTCAGGAC AAATTGATGC AGACAACAAT GGATATGTTG ATGTAGCGGT TGGTGCATTT 
CAGTCTGATT CTGCAGTGTT GCTAAGGACA CGGCCTGTAG TGATTGTTGA AGCATCTTTA 
AGCCATCCTG AGTCAGTAAA TAGAACAAAA TTTGACTGTA CTGAAAATGG ACTTCTGTCT 
GTGTGCGTGG ATCTTAAACT GTGTTTCTCA TATAAGGGCA AAGAGGTCCC AGGCTACATC 
GTTTTGTTTT ACAATGTGAG CTTGGATGTG CACAGGAAGG CCGAGTCTCC GTCAAGATTT 
TATTTCTTCT CCAATGGGAC TTCTGACATG ATTACAAGAA GCATACGAGT TTCAAGCAGT 
GGAGAGAAAT GTAGGACACA CCAGGCATTC ATGCGGAAAG ACGTGCGAGA CATCCTCACT 
CCCATTCAGG TTGAGGCCAC ATACCACCTT GGGCATCATG TGATCACCAA ACGAAACACA 
GAGGAATTCC CACCCCTCCA GCCGATCCTT CAGCAGAAGA AAGAAAAAGA CATTATTAGA 
AAAACGATAA ACTTCGCAAG GTTTTGTGCC TATGAAAATT GTTCTGCTGA TCTGCAGGTT 
TCTGCAAAAG TTGGGTTTTT GAAGCCATAT GAAAATAAAA CCTATCTTGC TGTTGGGAGC 
ATGAAGACCA TAATGCTAAA TGTGTCCTTG TTCAATGCTG GAGATGATGC TTATGAAACT 
ACTCTGAATG TCCAACTCCC CACAGGCCTT TATTTCATTA AGATCTTAGA CCTGGAAGAG 
AAACAAATAA ACTGCGACGT GACAGAGAGC TCAGGCGTAG TTAAGCTTGC CTGCAGCCTC 
GGTTACATAT ACGTGGATCG TCTCTCAAGG ATAGACATTA GCTTTCTCCT GGATGTGAGC 
TCACTCAGCA GGGCCCATGA GGACCTCAGC ATCAACGTGC ATGCCTCCTG TGAAAATGAG 
GGTAAATTGG ACCAAGTGAA AGACAACAGA GTGACCTTAG TGATACCTCT AAGGTATGAA 
GTTATGCTGG CTGTTCACGG GTTTGTGACC CCAACTTCGT TTGTGTATGG ATCCAGTGAA 
GAAAACGAGC CAGAAATGTG CATGGCAGAG AAATTGAACC TCACTTTCCA TGTTATAAAC 
ACTGGCATTA GCATGGCTCC AAATGTTAGT GTGAAAATAA TGGTACCAAA TTCTTTTCTC 
CCTCGAAATG ATAAGTTGTT CAACGTTTTG GATGTCCAGG CAACTATGGG GCAGTGCCAA 
TTTGAACACT ATGGACGAGA GTGTGCATTA GCACAGCAAA AAGGCATAGC GGGGACCTTG 
ACCGATTTGG TCAAATTTCT ATCAAAGACT GATAAGAGAC TCCTGTATTG CATGAAAGCT 
GATCGACACT GTTTAGATTT CTTATGTAAT TTCGGGAAAA TGGAAAGTGG GAAGGAAGCC 
AGCGTTCACA TTCAGCTGGA AGGCAGGCCA TCCATCTTGG AAATGGATGA GACCTCATCG 
CTCAAATTTG AAATAAAAGC AACAGCTTTT CCAGAGCCAC ACCCAAAAGT TATTGAGCTC 
AATAAGGATG AGAACATTGC CCATGTTTAC TTGGAAGGGC TCCATCATCA AAGACCCAAA 
CGGCATTTCA CCATCGTGAT TGTCACCATC AGCTTGCTCC TTGGACTTAT TGTACTTTTA 
TTAATTTCAT GTGTTATGTG GAAGGCTGGA TTTTTTAAAA GGCAGTACAA ATCTATCCTA 
CAAGAAGAAA ACAGGAGAGA CAGCTGGAGT TATGTCAACA GCAAAAGCAA TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga4 ( XM_017591706.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447195.1 Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa60386
Clone ID Related Accession (Same CDS sequence)	XM_017591706.1
Accession Version	XM_017591706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	integrin alpha-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGGTGCT ACAGGGGAGG TTAAAAGCTT GGGGCTGGAT TACTTTCTCT 
CCTTCTACAT GGATTCTAAG GGAAAAGCCT GTAGCCTCTG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447195.1
CDS	5914..7941
Translation	MAAEAMCRPRPRGVALQEAVMLLLYFGVHFRVPTGHSYNLDPESALLYQGPSGTLFGYSVVLHSHGTKRWLIVGAPTASWLANASVVNPGAIYRCRIGKNPNQICEQLQLGSPSGEPCGKTCLEERDNQWLGVTISRQPGENGSIVTCGHRWKNIFYMKNNNKLPTGVCYGMPSDLRTELSKRMAPCYQDFVRKFGENFASCQAGISSFYTQDLIVMGAPGSSYWTGSVFVYNITTNKYKAFVDRQNQVKFGSYLGYSVGAGHFRNPHTTEVVGGAPQHEQIGKAYIFSIDESELTIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGMGAVMVEMERVLVGSDKYAARFGESMANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQRIEGRQISQSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDCTENGLLSVCVDLKLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDMITRSIRVSSSGEKCRTHQAFMRKDVRDILTPIQVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDIIRKTINFARFCAYENCSADLQVSAKVGFLKVLQGRLKAWGWITFSPSTWILREKPVASAS

Target ORF information:

RefSeq Version	XM_017591706.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591706.1

ORF Insert Sequence:

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGGTGCT ACAGGGGAGG TTAAAAGCTT GGGGCTGGAT TACTTTCTCT 
CCTTCTACAT GGATTCTAAG GGAAAAGCCT GTAGCCTCTG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga4 ( NM_001107737.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101207.1 Rattus norvegicus integrin subunit alpha 4 (Itga4), mRNA.

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CloneID	ORa05793
Clone ID Related Accession (Same CDS sequence)	NM_001107737.1
Accession Version	NM_001107737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	integrin alpha-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_230033.4, XM_001067316.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGCCATA TGAAAATAAA ACCTATCTTG CTGTTGGGAG CATGAAGACC 
ATAATGCTAA ATGTGTCCTT GTTCAATGCT GGAGATGATG CTTATGAAAC TACTCTGAAT 
GTCCAACTCC CCACAGGCCT TTATTTCATT AAGATCTTAG ACCTGGAAGA GAAACAAATA 
AACTGCGACG TGACAGAGAG CTCAGGCGTA GTTAAGCTTG CCTGCAGCCT CGGTTACATA 
TACGTGGATC GTCTCTCAAG GATAGACATT AGCTTTCTCC TGGATGTGAG CTCACTCAGC 
AGGGCCCATG AGGACCTCAG CATCAACGTG CATGCCTCCT GTGAAAATGA GGGTAAATTG 
GACCAAGTGA AAGACAACAG AGTGACCTTA GTGATACCTC TAAGGTATGA AGTTATGCTG 
GCTGTTCACG GGTTTGTGAC CCCAACTTCG TTTGTGTATG GATCCAGTGA AGAAAACGAG 
CCAGAAATGT GCATGGCAGA GAAATTGAAC CTCACTTTCC ATGTTATAAA CACTGGCATT 
AGCATGGCTC CAAATGTTAG TGTGAAAATA ATGGTACCAA ATTCTTTTCT CCCTCGAAAT 
GATAAGTTGT TCAACGTTTT GGATGTCCAG GCAACTATGG GGCAGTGCCA ATTTGAACAC 
TATGGACGAG AGTGTGCATT AGCACAGCAA AAAGGCATAG CGGGGACCTT GACCGATTTG 
GTCAAATTTC TATCAAAGAC TGATAAGAGA CTCCTGTATT GCATGAAAGC TGATCGACAC 
TGTTTAGATT TCTTATGTAA TTTCGGGAAA ATGGAAAGTG GGAAGGAAGC CAGCGTTCAC 
ATTCAGCTGG AAGGCAGGCC ATCCGTCTTG GAAATGGATG AGACCTCATC GCTCAAATTT 
GAAATAAAAG CAACAGCTTT TCCAGAGCCA CACCCAAAAG TTATTGAGCT CAATAAGGAT 
GAGAACATTG CCCATGTTTA CTTGGAAGGG CTCCATCATC AAAGACCCAA ACGGCATTTC 
ACCATCGTGA TTGTCACCAT CAGCTTGCTC CTTGGACTTA TTGTACTTTT ATTAATTTCA 
TGTGTTATGT GGAAGGCTGG ATTTTTTAAA AGGCAGTACA AATCTATCCT ACAAGAAGAA 
AACAGGAGAG ACAGCTGGAG TTATGTCAAC AGCAAAAGCA ATGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101207.1
CDS	236..3346
Translation	MAAEAMCRPRPRGVALQEAVMLLLYFGVHFRVPTGHSYNLDPESALLYQGPSGTLFGYSVVLHSHGTKRWLIVGAPTASWLANASVVNPGAIYRCRIGKNPNQICEQLQLGSPSGEPCGKTCLEERDNQWLGVTISRQPGENGSIVTCGHRWKNIFYMKNNNKLPTGVCYGMPSDLRTELSKRMAPCYQDFVRKFGENFASCQAGISSFYTQDLIVMGAPGSSYWTGSVFVYNITTNKYKAFVDRQNQVKFGSYLGYSVGAGHFRNPHTTEVVGGAPQHEQIGKAYIFSIDESELTIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGMGAVMVEMERVLVGSDKYAARFGESMANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQRIEGRQISQSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDCTENGLLSVCVDLKLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDMITRSIRVSSSGEKCRTHQAFMRKDVRDILTPIQVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDIIRKTINFARFCAYENCSADLQVSAKVGFLKPYENKTYLAVGSMKTIMLNVSLFNAGDDAYETTLNVQLPTGLYFIKILDLEEKQINCDVTESSGVVKLACSLGYIYVDRLSRIDISFLLDVSSLSRAHEDLSINVHASCENEGKLDQVKDNRVTLVIPLRYEVMLAVHGFVTPTSFVYGSSEENEPEMCMAEKLNLTFHVINTGISMAPNVSVKIMVPNSFLPRNDKLFNVLDVQATMGQCQFEHYGRECALAQQKGIAGTLTDLVKFLSKTDKRLLYCMKADRHCLDFLCNFGKMESGKEASVHIQLEGRPSVLEMDETSSLKFEIKATAFPEPHPKVIELNKDENIAHVYLEGLHHQRPKRHFTIVIVTISLLLGLIVLLLISCVMWKAGFFKRQYKSILQEENRRDSWSYVNSKSNDD

Target ORF information:

RefSeq Version	NM_001107737.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus integrin subunit alpha 4 (Itga4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107737.1

ORF Insert Sequence:

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGCCATA TGAAAATAAA ACCTATCTTG CTGTTGGGAG CATGAAGACC 
ATAATGCTAA ATGTGTCCTT GTTCAATGCT GGAGATGATG CTTATGAAAC TACTCTGAAT 
GTCCAACTCC CCACAGGCCT TTATTTCATT AAGATCTTAG ACCTGGAAGA GAAACAAATA 
AACTGCGACG TGACAGAGAG CTCAGGCGTA GTTAAGCTTG CCTGCAGCCT CGGTTACATA 
TACGTGGATC GTCTCTCAAG GATAGACATT AGCTTTCTCC TGGATGTGAG CTCACTCAGC 
AGGGCCCATG AGGACCTCAG CATCAACGTG CATGCCTCCT GTGAAAATGA GGGTAAATTG 
GACCAAGTGA AAGACAACAG AGTGACCTTA GTGATACCTC TAAGGTATGA AGTTATGCTG 
GCTGTTCACG GGTTTGTGAC CCCAACTTCG TTTGTGTATG GATCCAGTGA AGAAAACGAG 
CCAGAAATGT GCATGGCAGA GAAATTGAAC CTCACTTTCC ATGTTATAAA CACTGGCATT 
AGCATGGCTC CAAATGTTAG TGTGAAAATA ATGGTACCAA ATTCTTTTCT CCCTCGAAAT 
GATAAGTTGT TCAACGTTTT GGATGTCCAG GCAACTATGG GGCAGTGCCA ATTTGAACAC 
TATGGACGAG AGTGTGCATT AGCACAGCAA AAAGGCATAG CGGGGACCTT GACCGATTTG 
GTCAAATTTC TATCAAAGAC TGATAAGAGA CTCCTGTATT GCATGAAAGC TGATCGACAC 
TGTTTAGATT TCTTATGTAA TTTCGGGAAA ATGGAAAGTG GGAAGGAAGC CAGCGTTCAC 
ATTCAGCTGG AAGGCAGGCC ATCCGTCTTG GAAATGGATG AGACCTCATC GCTCAAATTT 
GAAATAAAAG CAACAGCTTT TCCAGAGCCA CACCCAAAAG TTATTGAGCT CAATAAGGAT 
GAGAACATTG CCCATGTTTA CTTGGAAGGG CTCCATCATC AAAGACCCAA ACGGCATTTC 
ACCATCGTGA TTGTCACCAT CAGCTTGCTC CTTGGACTTA TTGTACTTTT ATTAATTTCA 
TGTGTTATGT GGAAGGCTGG ATTTTTTAAA AGGCAGTACA AATCTATCCT ACAAGAAGAA 
AACAGGAGAG ACAGCTGGAG TTATGTCAAC AGCAAAAGCA ATGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itga4 ( XM_017591704.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447193.1 Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X1, mRNA.

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CloneID	ORa60384
Clone ID Related Accession (Same CDS sequence)	XM_017591704.1
Accession Version	XM_017591704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	integrin alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGCCATA TGAAAATAAA ACCTATCTTG CTGTTGGGAG CATGAAGACC 
ATAATGCTAA ATGTGTCCTT GTTCAATGCT GGAGATGATG CTTATGAAAC TACTCTGAAT 
GTCCAACTCC CCACAGGCCT TTATTTCATT AAGATCTTAG ACCTGGAAGA GAAACAAATA 
AACTGCGACG TGACAGAGAG CTCAGGCGTA GTTAAGCTTG CCTGCAGCCT CGGTTACATA 
TACGTGGATC GTCTCTCAAG GATAGACATT AGCTTTCTCC TGGATGTGAG CTCACTCAGC 
AGGGCCCATG AGGACCTCAG CATCAACGTG CATGCCTCCT GTGAAAATGA GGGTAAATTG 
GACCAAGTGA AAGACAACAG AGTGACCTTA GTGATACCTC TAAGGTATGA AGTTATGCTG 
GCTGTTCACG GGTTTGTGAC CCCAACTTCG TTTGTGTATG GATCCAGTGA AGAAAACGAG 
CCAGAAATGT GCATGGCAGA GAAATTGAAC CTCACTTTCC ATGTTATAAA CACTGGCATT 
AGCATGGCTC CAAATGTTAG TGTGAAAATA ATGGTACCAA ATTCTTTTCT CCCTCGAAAT 
GATAAGTTGT TCAACGTTTT GGATGTCCAG GCAACTATGG GGCAGTGCCA ATTTGAACAC 
TATGGACGAG AGTGTGCATT AGCACAGCAA AAAGGCATAG CGGGGACCTT GACCGATTTG 
GTCAAATTTC TATCAAAGAC TGATAAGAGA CTCCTGTATT GCATGAAAGC TGATCGACAC 
TGTTTAGATT TCTTATGTAA TTTCGGGAAA ATGGAAAGTG GGAAGGAAGC CAGCGTTCAC 
ATTCAGCTGG AAGGCAGGCC ATCCATCTTG GAAATGGATG AGACCTCATC GCTCAAATTT 
GAAATAAAAG CAACAGCTTT TCCAGAGCCA CACCCAAAAG TTATTGAGCT CAATAAGGAT 
GAGAACATTG CCCATGTTTA CTTGGAAGGG CTCCATCATC AAAGACCCAA ACGGCATTTC 
ACCATCGTGA TTGTCACCAT CAGCTTGCTC CTTGGACTTA TTGTACTTTT ATTAATTTCA 
TGTGTTATGT GGAAGGCTGG ATTTTTTAAA AGGCAGTACA AATCTATCCT ACAAGAAGAA 
AACAGGAGAG ACAGCTGGAG TTATGTCAAC AGCAAAAGCA ATGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447193.1
CDS	5914..9024
Translation	MAAEAMCRPRPRGVALQEAVMLLLYFGVHFRVPTGHSYNLDPESALLYQGPSGTLFGYSVVLHSHGTKRWLIVGAPTASWLANASVVNPGAIYRCRIGKNPNQICEQLQLGSPSGEPCGKTCLEERDNQWLGVTISRQPGENGSIVTCGHRWKNIFYMKNNNKLPTGVCYGMPSDLRTELSKRMAPCYQDFVRKFGENFASCQAGISSFYTQDLIVMGAPGSSYWTGSVFVYNITTNKYKAFVDRQNQVKFGSYLGYSVGAGHFRNPHTTEVVGGAPQHEQIGKAYIFSIDESELTIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGMGAVMVEMERVLVGSDKYAARFGESMANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQRIEGRQISQSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDCTENGLLSVCVDLKLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDMITRSIRVSSSGEKCRTHQAFMRKDVRDILTPIQVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDIIRKTINFARFCAYENCSADLQVSAKVGFLKPYENKTYLAVGSMKTIMLNVSLFNAGDDAYETTLNVQLPTGLYFIKILDLEEKQINCDVTESSGVVKLACSLGYIYVDRLSRIDISFLLDVSSLSRAHEDLSINVHASCENEGKLDQVKDNRVTLVIPLRYEVMLAVHGFVTPTSFVYGSSEENEPEMCMAEKLNLTFHVINTGISMAPNVSVKIMVPNSFLPRNDKLFNVLDVQATMGQCQFEHYGRECALAQQKGIAGTLTDLVKFLSKTDKRLLYCMKADRHCLDFLCNFGKMESGKEASVHIQLEGRPSILEMDETSSLKFEIKATAFPEPHPKVIELNKDENIAHVYLEGLHHQRPKRHFTIVIVTISLLLGLIVLLLISCVMWKAGFFKRQYKSILQEENRRDSWSYVNSKSNDD

Target ORF information:

RefSeq Version	XM_017591704.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus integrin subunit alpha 4 (Itga4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591704.1

ORF Insert Sequence:

ATGGCTGCGG AAGCGATGTG CAGACCGAGG CCCCGAGGGG TCGCCCTCCA GGAAGCGGTG 
ATGCTGTTGC TGTACTTCGG GGTACACTTC AGGGTACCAA CCGGGCACTC CTACAACCTG 
GACCCGGAGA GTGCACTGCT GTACCAGGGC CCCTCCGGCA CGCTGTTTGG CTACTCGGTG 
GTGCTGCACA GCCACGGGAC GAAGCGCTGG CTCATTGTGG GGGCGCCCAC TGCCAGCTGG 
CTCGCCAATG CCTCGGTGGT CAATCCTGGG GCGATTTACA GATGCAGGAT CGGAAAGAAT 
CCAAACCAGA TCTGCGAACA GCTCCAGCTG GGAAGCCCCA GTGGAGAACC CTGTGGGAAG 
ACGTGCTTGG AAGAGAGAGA TAATCAGTGG CTGGGAGTGA CAATTTCCAG ACAGCCAGGA 
GAAAATGGAT CTATCGTGAC TTGTGGGCAC AGGTGGAAAA ATATATTTTA CATGAAGAAC 
AATAATAAAC TCCCCACTGG TGTTTGCTAT GGCATGCCTT CTGATTTGCG GACAGAACTA 
AGTAAAAGGA TGGCCCCCTG TTACCAAGAT TTTGTGAGAA AATTTGGAGA AAATTTTGCA 
TCGTGTCAAG CTGGAATATC TAGTTTTTAC ACACAGGATT TGATTGTGAT GGGGGCCCCG 
GGATCATCGT ACTGGACCGG CTCCGTCTTT GTCTACAACA TAACTACGAA CAAATACAAG 
GCATTTGTAG ACAGACAGAA TCAAGTAAAA TTTGGAAGCT ACTTAGGCTA CTCAGTAGGA 
GCTGGTCATT TCCGAAATCC ACATACTACC GAAGTCGTGG GAGGAGCCCC TCAACACGAA 
CAGATAGGAA AAGCATATAT ATTCAGCATT GATGAAAGCG AACTGACCAT CGTATATGAA 
ATGAAAGGTA AAAAGCTTGG CTCTTACTTT GGAGCTTCTG TCTGTGCGGT GGACCTCAAT 
GCAGATGGCT TCTCAGATCT CCTTGTTGGA GCGCCTATGC AGAGCACCAT CAGGGAGGAA 
GGAAGAGTGT TCGTGTACAT CAACTCTGGC ATGGGAGCTG TGATGGTTGA AATGGAAAGA 
GTGCTCGTCG GAAGTGACAA ATATGCTGCC AGGTTTGGGG AATCCATGGC AAATCTTGGC 
GACATTGACA ATGATGGCTT TGAAGATATT GCTATTGGCG CACCACAAGA AGATGACTTG 
CGAGGCGCTG TCTACATTTA CAATGGCCGA GTAGATGGCA TCTCCTCTAC ATACTCACAG 
AGAATCGAAG GACGTCAAAT CAGCCAGTCA CTAAGGATGT TTGGACAGTC TATCTCAGGA 
CAAATTGATG CAGACAACAA TGGATATGTT GATGTAGCGG TTGGTGCATT TCAGTCTGAT 
TCTGCAGTGT TGCTAAGGAC ACGGCCTGTA GTGATTGTTG AAGCATCTTT AAGCCATCCT 
GAGTCAGTAA ATAGAACAAA ATTTGACTGT ACTGAAAATG GACTTCTGTC TGTGTGCGTG 
GATCTTAAAC TGTGTTTCTC ATATAAGGGC AAAGAGGTCC CAGGCTACAT CGTTTTGTTT 
TACAATGTGA GCTTGGATGT GCACAGGAAG GCCGAGTCTC CGTCAAGATT TTATTTCTTC 
TCCAATGGGA CTTCTGACAT GATTACAAGA AGCATACGAG TTTCAAGCAG TGGAGAGAAA 
TGTAGGACAC ACCAGGCATT CATGCGGAAA GACGTGCGAG ACATCCTCAC TCCCATTCAG 
GTTGAGGCCA CATACCACCT TGGGCATCAT GTGATCACCA AACGAAACAC AGAGGAATTC 
CCACCCCTCC AGCCGATCCT TCAGCAGAAG AAAGAAAAAG ACATTATTAG AAAAACGATA 
AACTTCGCAA GGTTTTGTGC CTATGAAAAT TGTTCTGCTG ATCTGCAGGT TTCTGCAAAA 
GTTGGGTTTT TGAAGCCATA TGAAAATAAA ACCTATCTTG CTGTTGGGAG CATGAAGACC 
ATAATGCTAA ATGTGTCCTT GTTCAATGCT GGAGATGATG CTTATGAAAC TACTCTGAAT 
GTCCAACTCC CCACAGGCCT TTATTTCATT AAGATCTTAG ACCTGGAAGA GAAACAAATA 
AACTGCGACG TGACAGAGAG CTCAGGCGTA GTTAAGCTTG CCTGCAGCCT CGGTTACATA 
TACGTGGATC GTCTCTCAAG GATAGACATT AGCTTTCTCC TGGATGTGAG CTCACTCAGC 
AGGGCCCATG AGGACCTCAG CATCAACGTG CATGCCTCCT GTGAAAATGA GGGTAAATTG 
GACCAAGTGA AAGACAACAG AGTGACCTTA GTGATACCTC TAAGGTATGA AGTTATGCTG 
GCTGTTCACG GGTTTGTGAC CCCAACTTCG TTTGTGTATG GATCCAGTGA AGAAAACGAG 
CCAGAAATGT GCATGGCAGA GAAATTGAAC CTCACTTTCC ATGTTATAAA CACTGGCATT 
AGCATGGCTC CAAATGTTAG TGTGAAAATA ATGGTACCAA ATTCTTTTCT CCCTCGAAAT 
GATAAGTTGT TCAACGTTTT GGATGTCCAG GCAACTATGG GGCAGTGCCA ATTTGAACAC 
TATGGACGAG AGTGTGCATT AGCACAGCAA AAAGGCATAG CGGGGACCTT GACCGATTTG 
GTCAAATTTC TATCAAAGAC TGATAAGAGA CTCCTGTATT GCATGAAAGC TGATCGACAC 
TGTTTAGATT TCTTATGTAA TTTCGGGAAA ATGGAAAGTG GGAAGGAAGC CAGCGTTCAC 
ATTCAGCTGG AAGGCAGGCC ATCCATCTTG GAAATGGATG AGACCTCATC GCTCAAATTT 
GAAATAAAAG CAACAGCTTT TCCAGAGCCA CACCCAAAAG TTATTGAGCT CAATAAGGAT 
GAGAACATTG CCCATGTTTA CTTGGAAGGG CTCCATCATC AAAGACCCAA ACGGCATTTC 
ACCATCGTGA TTGTCACCAT CAGCTTGCTC CTTGGACTTA TTGTACTTTT ATTAATTTCA 
TGTGTTATGT GGAAGGCTGG ATTTTTTAAA AGGCAGTACA AATCTATCCT ACAAGAAGAA 
AACAGGAGAG ACAGCTGGAG TTATGTCAAC AGCAAAAGCA ATGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-30s Family Inhibit the Proliferation and Apoptosis in Human Coronary Artery Endothelial Cells Through Targeting the 3'UTR Region of ITGA4 and PLCG1.
Journal of cardiovascular pharmacology68(5)327-333(2016 Nov)
Ma F,Li T,Zhang H,Wu G

Disruption of disulfide restriction at integrin knees induces activation and ligand-independent signaling of α₄β₇.
Journal of cell science126(Pt 21)5030-41(2013 Nov)
Zhang K,Pan Y,Qi J,Yue J,Zhang M,Xu C,Li G,Chen J

GPR15-mediated homing controls immune homeostasis in the large intestine mucosa.
Science (New York, N.Y.)340(6139)1456-9(2013 Jun)
Kim SV,Xiang WV,Kwak C,Yang Y,Lin XW,Ota M,Sarpel U,Rifkin DB,Xu R,Littman DR

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K

A positive feedback loop involving Gcm1 and Fzd5 directs chorionic branching morphogenesis in the placenta.
PLoS biology11(4)e1001536(2013)
Lu J,Zhang S,Nakano H,Simmons DG,Wang S,Kong S,Wang Q,Shen L,Tu Z,Wang W,Wang B,Wang H,Wang Y,van Es JH,Clevers H,Leone G,Cross JC,Wang H

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Itga4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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