Plk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plk4
Entrez Gene ID	310344
Full Name	polo-like kinase 4
General protein information	Preferred Names polo-like kinase 4 Names serine/threonine-protein kinase PLK4 PLK-4 serine/threonine-protein kinase Sak
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q25

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232300.1	XP_006232362.1	serine/threonine-protein kinase PLK4 isoform X3
NM_001107669.1	NP_001101139.1	serine/threonine-protein kinase PLK4
XM_006232299.3	XP_006232361.1	serine/threonine-protein kinase PLK4 isoform X2
XM_017590857.1	XP_017446346.1	serine/threonine-protein kinase PLK4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLK4	XP_517431.4
Rattus norvegicus (Norway rat)	Plk4	NP_001101139.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012499	XP_307136.4
Macaca mulatta (Rhesus monkey)	PLK4	XP_001105577.1
Mus musculus (house mouse)	Plk4	NP_035625.2
Canis lupus familiaris (dog)	PLK4	XP_533295.2
Bos taurus (cattle)	PLK4	NP_001076896.1
Danio rerio (zebrafish)	plk4	NP_001112364.1
Drosophila melanogaster (fruit fly)	SAK	NP_649324.1
Xenopus tropicalis (tropical clawed frog)	plk4	NP_001122128.1
Homo sapiens (human)	PLK4	NP_055079.3
Gallus gallus (chicken)	LOC422497	XP_004941066.1

Gene Ontology
Bibliography

Related articles in PubMed

KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification.
Fournier M, Orpinell M, Grauffel C, Scheer E, Garnier JM, Ye T, Chavant V, Joint M, Esashi F, Dejaegere A, Gönczy P, Tora L
Nature communications713227(2016 10)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Park SY, Park JE, Kim TS, Kim JH, Kwak MJ, Ku B, Tian L, Murugan RN, Ahn M, Komiya S, Hojo H, Kim NH, Kim BY, Bang JK, Erikson RL, Lee KW, Kim SJ, Oh BH, Yang W, Lee KS
Nature structural & molecular biology21(8)696-703(2014 Aug)

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X, Zhu X
Nature cell biology15(12)1434-44(2013 Dec)

Polo-like kinase is required for synaptonemal complex disassembly and phosphorylation in mouse spermatocytes.
Jordan PW, Karppinen J, Handel MA
Journal of cell science125(Pt 21)5061-72(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05541	NM_001107669.1 Latest version!	Rattus norvegicus polo-like kinase 4 (Plk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa24649	XM_006232299.3 Latest version!	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa24650	XM_006232300.1 Latest version!	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa59742	XM_017590857.1 Latest version!	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plk4 ( NM_001107669.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101139.1 Rattus norvegicus polo-like kinase 4 (Plk4), mRNA.

Price & Availability↑

CloneID	ORa05541
Clone ID Related Accession (Same CDS sequence)	NM_001107669.1
Accession Version	NM_001107669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541865600000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase PLK4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473961.1. On or before Oct 4, 2007 this sequence version replaced XM_227064.4, XM_001070843.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT GCATCGGAGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTTGGTAAA 
GGATCATTTG CTGGTCTCTA TAATTACTTT GAAGATAACA ATTACGTATA CCTGGTATTA 
GAAATGTGCC ACAACGGAGA AATGAACAGG TACCTAAAGA ACAGAATGAA GCCCAGGCAC 
TTCATGCACC AGATCATCAC AGGAATGCTG TATCTTCATT CTCACGGCAT ACTACACCGC 
GACCTCACAC TCTCTAACAT CTTACTTACA CGGAATATGA ACATAAAAAT TGCTGACTTT 
GGACTAGCAA CACAATTGAA AATGCCACAT GAGAAGCATT ACACCCTGTG TGGAACCCCT 
AATTATATTT CACCAGAAAT TGCAACTCGA AGTGCACATG GACTTGAATC TGATATTTGG 
TCATTGGGCT GTATGTTTTA TACATTACTT ATTGGAAGAC CACCTTTTGA CACTGATACA 
GTCAAAAACA CATTGAACAA AGTAGTATTG GCAGATTATG AAATGCCAGC CTTTTTGTCA 
CGAGAGGCCC AGGACCTTAT CCACCAATTA CTTCGTAGAA ACCCTGCAGA TCGGTTAAGT 
CTGTCTTCTG TGTTGGACCA TCCTTTTATG TCACGAAATC CTTCAACAAA GAGTAAAGAC 
TTAGGGACTG TAGAGGACTC AATGGATAGT GGGCATGCCA CACTTTCCAC AACAATTACA 
GCTTCTTCCG GAACCAGTTT GAGTGGTAGC CTACTTGACA GAAGAAGACT TTTGGTTGGT 
CAACCACTTC CAAATAAAAT TACTGTATTT CAAAAAAATA AAAATTCAAG TGACTTTTCT 
TCAGGAGATG GAAGTAATTT TTGTACTCAA TGGGGAAATC CAGAACAAGA AGCTAATAAT 
AGGGGGCGGG GAAGAGTAAT TGAAGATGCA GAAGAAAGGC CACATTCTCG ATACCTTCGC 
AGAGCTCATT CCTCTGATAG ATCCAACCCT TCTAATCAGT CTCGAGCAAA AACATACTCA 
ATAGAGCGGT GTCACTCAGT AGAAATGCTT TCAAAGCCCA GAAGATCATC ACTGGATGAA 
ACTAAACACA GTTCCAATCA TCATTGTCTA GGGAAAACTC CTTTTCCATT TGCAGACCAG 
ACACCTCAGA TGGAGATAGT ACAACAGTGG TTTGGGAATC TGCAAATGAA TGGAGAAACT 
AGTGAGCACA ACACCATTAG CCCAAACAGA GATTTCCAGG ACTATCCTGA TGTGCAGGAC 
ACATTAAGAA ATACTTGGAC TGACACAAGA GCCAGCAAGA ATTCCGATAA TTCTGCTAAC 
GTTCATCCTG CAAAGCAGCT GAGTACCATG AAATACATGA CTGCACATCA CCATAAACCT 
GAGATCATGC AGCAAGAGCT GGCCATACAT CCTCATTCTG AACAAAACAA GAGTAGAAGT 
ATGGAGTCAA CATTGGGTTA CAGGAAACCT ACCTTAAGAA GCATTACCTC TCCTTTGGTT 
GCTCACAGAC TAAAACCAAT CAGACAGAAA ACCAAAAAGG CTGTGGTGAG CATCCTTGAT 
TCAGAGGAGG TGTGTGTGGA GCTTCTAAAA GAGTGTACAT CTGAAGGACA TGTGAAAGAA 
GTTCTTCAGA TATCTAGTGA TGGGACTACG ATCACCGTTT ATTACCCAAA TGATGGAAGA 
GGCTTTCCTC TTGCTGATAG ACCACCCTTG CCTACTGACA ACATCAGCAG GTACAGCTTT 
GACAGTTTAC CAGAAAAATA CTGGCGGAAA TATCAGTATG CTTCCAGATT CATTCAGCTT 
GTAAGGTCCA AAACTCCCAA AATCACTTAC TTTACAAGAT ATGCTAAATG TATTTTGATG 
GAAAATTCTC CTGGTGCTGA TTTTGAAGTT TGGTTTTATG ATGGAGCCAA AATACATAAA 
ACAGAAGATG TAATTCACAT AATTGAAAAA ACTGGGTTAT CTTACACTTT AAAAAATGAG 
AATGACTTTA CCAGCTTGAA AGAGGAAGTA AAAATCTATA TGGACCATGC CAATGAGGGT 
CACCGTACTT GCTTGGCACT GGAATCTGTA ATCTCTGAAG AGGAAAAAAG AAGCAGGGGC 
TCTTCATTCT TCCCAATAAT TGTAGGAAGA AAACCTGGTA CTACTAGTTC ACCTAAAGCC 
TTATCACCAC CTCCTGTGGA CCCAGGCTAC TCAAAGGGAG AGCAAGCATC ATCAAGCAGA 
TTGAGCGCGA ATAGTGCGGC TTTCCCAACA CAGACACCAG TACTCAGTCC TTCTGCTGTG 
ACAGTGGAAG GACCTGGCCA AACAGCAGCT ACTACAGGAA CAAGCATCTC TTCAAGTCTT 
CCTAAATCAG CACAGCTTTT GAAATCTGTT TTTGTGAAAA ATGTTGGTTG GGCTACACAG 
CTAACTAGTG GAGCTGTGTG GGTTCAGTTT AATGATGGGT CACAGTTGGT TGTCCAGGCA 
GGAGTATCTT CCATCAGTTA TACATCACCA GACGGTCAGA CAACTAGGTA TGGAGAAAAT 
GAAAAATTGC CTGAATACAT CAAACAGAAA TTGCAATGTC TTTCTTCCAT CCTTCTGATG 
TTTTCTAATC CAACTCCTAG TTTTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101139.1
CDS	231..2840
Translation	MAACIGERIEDFKVGNLLGKGSFAGLYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPRHFMHQIITGMLYLHSHGILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSTKSKDLGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRRLLVGQPLPNKITVFQKNKNSSDFSSGDGSNFCTQWGNPEQEANNRGRGRVIEDAEERPHSRYLRRAHSSDRSNPSNQSRAKTYSIERCHSVEMLSKPRRSSLDETKHSSNHHCLGKTPFPFADQTPQMEIVQQWFGNLQMNGETSEHNTISPNRDFQDYPDVQDTLRNTWTDTRASKNSDNSANVHPAKQLSTMKYMTAHHHKPEIMQQELAIHPHSEQNKSRSMESTLGYRKPTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELLKECTSEGHVKEVLQISSDGTTITVYYPNDGRGFPLADRPPLPTDNISRYSFDSLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPGADFEVWFYDGAKIHKTEDVIHIIEKTGLSYTLKNENDFTSLKEEVKIYMDHANEGHRTCLALESVISEEEKRSRGSSFFPIIVGRKPGTTSSPKALSPPPVDPGYSKGEQASSSRLSANSAAFPTQTPVLSPSAVTVEGPGQTAATTGTSISSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSILLMFSNPTPSFQ

Target ORF information:

RefSeq Version	NM_001107669.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polo-like kinase 4 (Plk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107669.1

ORF Insert Sequence:

ATGGCGGCGT GCATCGGAGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTTGGTAAA 
GGATCATTTG CTGGTCTCTA TAATTACTTT GAAGATAACA ATTACGTATA CCTGGTATTA 
GAAATGTGCC ACAACGGAGA AATGAACAGG TACCTAAAGA ACAGAATGAA GCCCAGGCAC 
TTCATGCACC AGATCATCAC AGGAATGCTG TATCTTCATT CTCACGGCAT ACTACACCGC 
GACCTCACAC TCTCTAACAT CTTACTTACA CGGAATATGA ACATAAAAAT TGCTGACTTT 
GGACTAGCAA CACAATTGAA AATGCCACAT GAGAAGCATT ACACCCTGTG TGGAACCCCT 
AATTATATTT CACCAGAAAT TGCAACTCGA AGTGCACATG GACTTGAATC TGATATTTGG 
TCATTGGGCT GTATGTTTTA TACATTACTT ATTGGAAGAC CACCTTTTGA CACTGATACA 
GTCAAAAACA CATTGAACAA AGTAGTATTG GCAGATTATG AAATGCCAGC CTTTTTGTCA 
CGAGAGGCCC AGGACCTTAT CCACCAATTA CTTCGTAGAA ACCCTGCAGA TCGGTTAAGT 
CTGTCTTCTG TGTTGGACCA TCCTTTTATG TCACGAAATC CTTCAACAAA GAGTAAAGAC 
TTAGGGACTG TAGAGGACTC AATGGATAGT GGGCATGCCA CACTTTCCAC AACAATTACA 
GCTTCTTCCG GAACCAGTTT GAGTGGTAGC CTACTTGACA GAAGAAGACT TTTGGTTGGT 
CAACCACTTC CAAATAAAAT TACTGTATTT CAAAAAAATA AAAATTCAAG TGACTTTTCT 
TCAGGAGATG GAAGTAATTT TTGTACTCAA TGGGGAAATC CAGAACAAGA AGCTAATAAT 
AGGGGGCGGG GAAGAGTAAT TGAAGATGCA GAAGAAAGGC CACATTCTCG ATACCTTCGC 
AGAGCTCATT CCTCTGATAG ATCCAACCCT TCTAATCAGT CTCGAGCAAA AACATACTCA 
ATAGAGCGGT GTCACTCAGT AGAAATGCTT TCAAAGCCCA GAAGATCATC ACTGGATGAA 
ACTAAACACA GTTCCAATCA TCATTGTCTA GGGAAAACTC CTTTTCCATT TGCAGACCAG 
ACACCTCAGA TGGAGATAGT ACAACAGTGG TTTGGGAATC TGCAAATGAA TGGAGAAACT 
AGTGAGCACA ACACCATTAG CCCAAACAGA GATTTCCAGG ACTATCCTGA TGTGCAGGAC 
ACATTAAGAA ATACTTGGAC TGACACAAGA GCCAGCAAGA ATTCCGATAA TTCTGCTAAC 
GTTCATCCTG CAAAGCAGCT GAGTACCATG AAATACATGA CTGCACATCA CCATAAACCT 
GAGATCATGC AGCAAGAGCT GGCCATACAT CCTCATTCTG AACAAAACAA GAGTAGAAGT 
ATGGAGTCAA CATTGGGTTA CAGGAAACCT ACCTTAAGAA GCATTACCTC TCCTTTGGTT 
GCTCACAGAC TAAAACCAAT CAGACAGAAA ACCAAAAAGG CTGTGGTGAG CATCCTTGAT 
TCAGAGGAGG TGTGTGTGGA GCTTCTAAAA GAGTGTACAT CTGAAGGACA TGTGAAAGAA 
GTTCTTCAGA TATCTAGTGA TGGGACTACG ATCACCGTTT ATTACCCAAA TGATGGAAGA 
GGCTTTCCTC TTGCTGATAG ACCACCCTTG CCTACTGACA ACATCAGCAG GTACAGCTTT 
GACAGTTTAC CAGAAAAATA CTGGCGGAAA TATCAGTATG CTTCCAGATT CATTCAGCTT 
GTAAGGTCCA AAACTCCCAA AATCACTTAC TTTACAAGAT ATGCTAAATG TATTTTGATG 
GAAAATTCTC CTGGTGCTGA TTTTGAAGTT TGGTTTTATG ATGGAGCCAA AATACATAAA 
ACAGAAGATG TAATTCACAT AATTGAAAAA ACTGGGTTAT CTTACACTTT AAAAAATGAG 
AATGACTTTA CCAGCTTGAA AGAGGAAGTA AAAATCTATA TGGACCATGC CAATGAGGGT 
CACCGTACTT GCTTGGCACT GGAATCTGTA ATCTCTGAAG AGGAAAAAAG AAGCAGGGGC 
TCTTCATTCT TCCCAATAAT TGTAGGAAGA AAACCTGGTA CTACTAGTTC ACCTAAAGCC 
TTATCACCAC CTCCTGTGGA CCCAGGCTAC TCAAAGGGAG AGCAAGCATC ATCAAGCAGA 
TTGAGCGCGA ATAGTGCGGC TTTCCCAACA CAGACACCAG TACTCAGTCC TTCTGCTGTG 
ACAGTGGAAG GACCTGGCCA AACAGCAGCT ACTACAGGAA CAAGCATCTC TTCAAGTCTT 
CCTAAATCAG CACAGCTTTT GAAATCTGTT TTTGTGAAAA ATGTTGGTTG GGCTACACAG 
CTAACTAGTG GAGCTGTGTG GGTTCAGTTT AATGATGGGT CACAGTTGGT TGTCCAGGCA 
GGAGTATCTT CCATCAGTTA TACATCACCA GACGGTCAGA CAACTAGGTA TGGAGAAAAT 
GAAAAATTGC CTGAATACAT CAAACAGAAA TTGCAATGTC TTTCTTCCAT CCTTCTGATG 
TTTTCTAATC CAACTCCTAG TTTTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plk4 ( XM_006232299.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232361.1 Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa24649
Clone ID Related Accession (Same CDS sequence)	XM_006232299.3
Accession Version	XM_006232299.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase PLK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232299.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT GCATCGGAGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTTGGTAAA 
GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATACACA CTGGTTTGGA AGTTGCAATC 
AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG 
GTGAAAATAC ATTGCCAGTT GAAACACCCC TCTGTCTTGG AGCTCTATAA TTACTTTGAA 
GATAACAATT ACGTATACCT GGTATTAGAA ATGTGCCACA ACGGAGAAAT GAACAGGTAC 
CTAAAGAACA GAATGAAGCC TTTCTCCGAA AGCGAAGCCA GGCACTTCAT GCACCAGATC 
ATCACAGGAA TGCTGTATCT TCATTCTCAC GGCATACTAC ACCGCGACCT CACACTCTCT 
AACATCTTAC TTACACGGAA TATGAACATA AAAATTGCTG ACTTTGGACT AGCAACACAA 
TTGAAAATGC CACATGAGAA GCATTACACC CTGTGTGGAA CCCCTAATTA TATTTCACCA 
GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCATT GGGCTGTATG 
TTTTATACAT TACTTATTGG AAGACCACCT TTTGACACTG ATACAGTCAA AAACACATTG 
AACAAAGTAG TATTGGCAGA TTATGAAATG CCAGCCTTTT TGTCACGAGA GGCCCAGGAC 
CTTATCCACC AATTACTTCG TAGAAACCCT GCAGATCGGT TAAGTCTGTC TTCTGTGTTG 
GACCATCCTT TTATGTCACG AAATCCTTCA ACAAAGAGTA AAGACTTAGG GACTGTAGAG 
GACTCAATGG ATAGTGGGCA TGCCACACTT TCCACAACAA TTACAGCTTC TTCCGGAACC 
AGTTTGAGTG GTAGCCTACT TGACAGAAGA AGACTTTTGG TTGGTCAACC ACTTCCAAAT 
AAAATTACTG TATTTCAAAA AAATAAAAAT TCAAGTGACT TTTCTTCAGG AGATGGAAGT 
AATTTTTGTA CTCAATGGGG AAATCCAGAA CAAGAAGCTA ATAATAGGGG GCGGGGAAGA 
GTAATTGAAG ATGCAGAAGA AAGGCCACAT TCTCGATACC TTCGCAGAGC TCATTCCTCT 
GATAGATCCA ACCCTTCTAA TCAGTCTCGA GCAAAAACAT ACTCAATAGA GCGGTGTCAC 
TCAGTAGAAA TGCTTTCAAA GCCCAGAAGA TCATCACTGG ATGAAACTAA ACACAGTTCC 
AATCATCATT GTCTAGGGAA AACTCCTTTT CCATTTGCAG ACCAGACACC TCAGATGGAG 
ATAGTACAAC AGTGGTTTGG GAATCTGCAA ATGAATGGAG AAACTAGTGA GCACAACACC 
ATTAGCCCAA ACAGAGATTT CCAGGACTAT CCTGATGTGC AGGACACATT AAGAAATACT 
TGGACTGACA CAAGAGCCAG CAAGAATTCC GATAATTCTG CTAACGTTCA TCCTGCAAAG 
CAGCTGAGTA CCATGAAATA CATGACTGCA CATCACCATA AACCTGAGAT CATGCAGCAA 
GAGCTGGCCA TACATCCTCA TTCTGAACAA AACAAGAGTA GAAGTATGGA GTCAACATTG 
GGTTACAGGA AACCTACCTT AAGAAGCATT ACCTCTCCTT TGGTTGCTCA CAGACTAAAA 
CCAATCAGAC AGAAAACCAA AAAGGCTGTG GTGAGCATCC TTGATTCAGA GGAGGTGTGT 
GTGGAGCTTC TAAAAGAGTG TACATCTGAA GGACATGTGA AAGAAGTTCT TCAGATATCT 
AGTGATGGGA CTACGATCAC CGTTTATTAC CCAAATGATG GAAGAGGCTT TCCTCTTGCT 
GATAGACCAC CCTTGCCTAC TGACAACATC AGCAGGTACA GCTTTGACAG TTTACCAGAA 
AAATACTGGC GGAAATATCA GTATGCTTCC AGATTCATTC AGCTTGTAAG GTCCAAAACT 
CCCAAAATCA CTTACTTTAC AAGATATGCT AAATGTATTT TGATGGAAAA TTCTCCTGGT 
GCTGATTTTG AAGTTTGGTT TTATGATGGA GCCAAAATAC ATAAAACAGA AGATGTAATT 
CACATAATTG AAAAAACTGG GTTATCTTAC ACTTTAAAAA ATGAGAATGA CTTTACCAGC 
TTGAAAGAGG AAGTAAAAAT CTATATGGAC CATGCCAATG AGGGTCACCG TACTTGCTTG 
GCACTGGAAT CTGTAATCTC TGAAGAGGAA AAAAGAAGCA GGGGCTCTTC ATTCTTCCCA 
ATAATTGTAG GAAGAAAACC TGGTACTACT AGTTCACCTA AAGCCTTATC ACCACCTCCT 
GTGGACCCAG GCTACTCAAA GGGAGAGCAA GCATCATCAA GCAGATTGAG CGCGAATAGT 
GCGGCTTTCC CAACACAGAC ACCAGTACTC AGTCCTTCTG CTGTGACAGT GGAAGGACCT 
GGCCAAACAG CAGCTACTAC AGGAACAAGC ATCTCTTCAA GTCTTCCTAA ATCAGCACAG 
CTTTTGAAAT CTGTTTTTGT GAAAAATGTT GGTTGGGCTA CACAGCTAAC TAGTGGAGCT 
GTGTGGGTTC AGTTTAATGA TGGGTCACAG TTGGTTGTCC AGGCAGGAGT ATCTTCCATC 
AGTTATACAT CACCAGACGG TCAGACAACT AGGTATGGAG AAAATGAAAA ATTGCCTGAA 
TACATCAAAC AGAAATTGCA ATGTCTTTCT TCCATCCTTC TGATGTTTTC TAATCCAACT 
CCTAGTTTTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232361.1
CDS	245..3019
Translation	MAACIGERIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSVLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPFSESEARHFMHQIITGMLYLHSHGILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSTKSKDLGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRRLLVGQPLPNKITVFQKNKNSSDFSSGDGSNFCTQWGNPEQEANNRGRGRVIEDAEERPHSRYLRRAHSSDRSNPSNQSRAKTYSIERCHSVEMLSKPRRSSLDETKHSSNHHCLGKTPFPFADQTPQMEIVQQWFGNLQMNGETSEHNTISPNRDFQDYPDVQDTLRNTWTDTRASKNSDNSANVHPAKQLSTMKYMTAHHHKPEIMQQELAIHPHSEQNKSRSMESTLGYRKPTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELLKECTSEGHVKEVLQISSDGTTITVYYPNDGRGFPLADRPPLPTDNISRYSFDSLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPGADFEVWFYDGAKIHKTEDVIHIIEKTGLSYTLKNENDFTSLKEEVKIYMDHANEGHRTCLALESVISEEEKRSRGSSFFPIIVGRKPGTTSSPKALSPPPVDPGYSKGEQASSSRLSANSAAFPTQTPVLSPSAVTVEGPGQTAATTGTSISSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSILLMFSNPTPSFQ

Target ORF information:

RefSeq Version	XM_006232299.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232299.3

ORF Insert Sequence:

ATGGCGGCGT GCATCGGAGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTTGGTAAA 
GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATACACA CTGGTTTGGA AGTTGCAATC 
AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG 
GTGAAAATAC ATTGCCAGTT GAAACACCCC TCTGTCTTGG AGCTCTATAA TTACTTTGAA 
GATAACAATT ACGTATACCT GGTATTAGAA ATGTGCCACA ACGGAGAAAT GAACAGGTAC 
CTAAAGAACA GAATGAAGCC TTTCTCCGAA AGCGAAGCCA GGCACTTCAT GCACCAGATC 
ATCACAGGAA TGCTGTATCT TCATTCTCAC GGCATACTAC ACCGCGACCT CACACTCTCT 
AACATCTTAC TTACACGGAA TATGAACATA AAAATTGCTG ACTTTGGACT AGCAACACAA 
TTGAAAATGC CACATGAGAA GCATTACACC CTGTGTGGAA CCCCTAATTA TATTTCACCA 
GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCATT GGGCTGTATG 
TTTTATACAT TACTTATTGG AAGACCACCT TTTGACACTG ATACAGTCAA AAACACATTG 
AACAAAGTAG TATTGGCAGA TTATGAAATG CCAGCCTTTT TGTCACGAGA GGCCCAGGAC 
CTTATCCACC AATTACTTCG TAGAAACCCT GCAGATCGGT TAAGTCTGTC TTCTGTGTTG 
GACCATCCTT TTATGTCACG AAATCCTTCA ACAAAGAGTA AAGACTTAGG GACTGTAGAG 
GACTCAATGG ATAGTGGGCA TGCCACACTT TCCACAACAA TTACAGCTTC TTCCGGAACC 
AGTTTGAGTG GTAGCCTACT TGACAGAAGA AGACTTTTGG TTGGTCAACC ACTTCCAAAT 
AAAATTACTG TATTTCAAAA AAATAAAAAT TCAAGTGACT TTTCTTCAGG AGATGGAAGT 
AATTTTTGTA CTCAATGGGG AAATCCAGAA CAAGAAGCTA ATAATAGGGG GCGGGGAAGA 
GTAATTGAAG ATGCAGAAGA AAGGCCACAT TCTCGATACC TTCGCAGAGC TCATTCCTCT 
GATAGATCCA ACCCTTCTAA TCAGTCTCGA GCAAAAACAT ACTCAATAGA GCGGTGTCAC 
TCAGTAGAAA TGCTTTCAAA GCCCAGAAGA TCATCACTGG ATGAAACTAA ACACAGTTCC 
AATCATCATT GTCTAGGGAA AACTCCTTTT CCATTTGCAG ACCAGACACC TCAGATGGAG 
ATAGTACAAC AGTGGTTTGG GAATCTGCAA ATGAATGGAG AAACTAGTGA GCACAACACC 
ATTAGCCCAA ACAGAGATTT CCAGGACTAT CCTGATGTGC AGGACACATT AAGAAATACT 
TGGACTGACA CAAGAGCCAG CAAGAATTCC GATAATTCTG CTAACGTTCA TCCTGCAAAG 
CAGCTGAGTA CCATGAAATA CATGACTGCA CATCACCATA AACCTGAGAT CATGCAGCAA 
GAGCTGGCCA TACATCCTCA TTCTGAACAA AACAAGAGTA GAAGTATGGA GTCAACATTG 
GGTTACAGGA AACCTACCTT AAGAAGCATT ACCTCTCCTT TGGTTGCTCA CAGACTAAAA 
CCAATCAGAC AGAAAACCAA AAAGGCTGTG GTGAGCATCC TTGATTCAGA GGAGGTGTGT 
GTGGAGCTTC TAAAAGAGTG TACATCTGAA GGACATGTGA AAGAAGTTCT TCAGATATCT 
AGTGATGGGA CTACGATCAC CGTTTATTAC CCAAATGATG GAAGAGGCTT TCCTCTTGCT 
GATAGACCAC CCTTGCCTAC TGACAACATC AGCAGGTACA GCTTTGACAG TTTACCAGAA 
AAATACTGGC GGAAATATCA GTATGCTTCC AGATTCATTC AGCTTGTAAG GTCCAAAACT 
CCCAAAATCA CTTACTTTAC AAGATATGCT AAATGTATTT TGATGGAAAA TTCTCCTGGT 
GCTGATTTTG AAGTTTGGTT TTATGATGGA GCCAAAATAC ATAAAACAGA AGATGTAATT 
CACATAATTG AAAAAACTGG GTTATCTTAC ACTTTAAAAA ATGAGAATGA CTTTACCAGC 
TTGAAAGAGG AAGTAAAAAT CTATATGGAC CATGCCAATG AGGGTCACCG TACTTGCTTG 
GCACTGGAAT CTGTAATCTC TGAAGAGGAA AAAAGAAGCA GGGGCTCTTC ATTCTTCCCA 
ATAATTGTAG GAAGAAAACC TGGTACTACT AGTTCACCTA AAGCCTTATC ACCACCTCCT 
GTGGACCCAG GCTACTCAAA GGGAGAGCAA GCATCATCAA GCAGATTGAG CGCGAATAGT 
GCGGCTTTCC CAACACAGAC ACCAGTACTC AGTCCTTCTG CTGTGACAGT GGAAGGACCT 
GGCCAAACAG CAGCTACTAC AGGAACAAGC ATCTCTTCAA GTCTTCCTAA ATCAGCACAG 
CTTTTGAAAT CTGTTTTTGT GAAAAATGTT GGTTGGGCTA CACAGCTAAC TAGTGGAGCT 
GTGTGGGTTC AGTTTAATGA TGGGTCACAG TTGGTTGTCC AGGCAGGAGT ATCTTCCATC 
AGTTATACAT CACCAGACGG TCAGACAACT AGGTATGGAG AAAATGAAAA ATTGCCTGAA 
TACATCAAAC AGAAATTGCA ATGTCTTTCT TCCATCCTTC TGATGTTTTC TAATCCAACT 
CCTAGTTTTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plk4 ( XM_006232300.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232362.1 Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24650
Clone ID Related Accession (Same CDS sequence)	XM_006232300.1
Accession Version	XM_006232300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase PLK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAAAG CTGGAATGGT ACAGAGAGTC CAAAATGAGG TGAAAATACA TTGCCAGTTG 
AAACACCCCT CTGTCTTGGA GCTCTATAAT TACTTTGAAG ATAACAATTA CGTATACCTG 
GTATTAGAAA TGTGCCACAA CGGAGAAATG AACAGGTACC TAAAGAACAG AATGAAGCCT 
TTCTCCGAAA GCGAAGCCAG GCACTTCATG CACCAGATCA TCACAGGAAT GCTGTATCTT 
CATTCTCACG GCATACTACA CCGCGACCTC ACACTCTCTA ACATCTTACT TACACGGAAT 
ATGAACATAA AAATTGCTGA CTTTGGACTA GCAACACAAT TGAAAATGCC ACATGAGAAG 
CATTACACCC TGTGTGGAAC CCCTAATTAT ATTTCACCAG AAATTGCAAC TCGAAGTGCA 
CATGGACTTG AATCTGATAT TTGGTCATTG GGCTGTATGT TTTATACATT ACTTATTGGA 
AGACCACCTT TTGACACTGA TACAGTCAAA AACACATTGA ACAAAGTAGT ATTGGCAGAT 
TATGAAATGC CAGCCTTTTT GTCACGAGAG GCCCAGGACC TTATCCACCA ATTACTTCGT 
AGAAACCCTG CAGATCGGTT AAGTCTGTCT TCTGTGTTGG ACCATCCTTT TATGTCACGA 
AATCCTTCAA CAAAGAGTAA AGACTTAGGG ACTGTAGAGG ACTCAATGGA TAGTGGGCAT 
GCCACACTTT CCACAACAAT TACAGCTTCT TCCGGAACCA GTTTGAGTGG TAGCCTACTT 
GACAGAAGAA GACTTTTGGT TGGTCAACCA CTTCCAAATA AAATTACTGT ATTTCAAAAA 
AATAAAAATT CAAGTGACTT TTCTTCAGGA GATGGAAGTA ATTTTTGTAC TCAATGGGGA 
AATCCAGAAC AAGAAGCTAA TAATAGGGGG CGGGGAAGAG TAATTGAAGA TGCAGAAGAA 
AGGCCACATT CTCGATACCT TCGCAGAGCT CATTCCTCTG ATAGATCCAA CCCTTCTAAT 
CAGTCTCGAG CAAAAACATA CTCAATAGAG CGGTGTCACT CAGTAGAAAT GCTTTCAAAG 
CCCAGAAGAT CATCACTGGA TGAAACTAAA CACAGTTCCA ATCATCATTG TCTAGGGAAA 
ACTCCTTTTC CATTTGCAGA CCAGACACCT CAGATGGAGA TAGTACAACA GTGGTTTGGG 
AATCTGCAAA TGAATGGAGA AACTAGTGAG CACAACACCA TTAGCCCAAA CAGAGATTTC 
CAGGACTATC CTGATGTGCA GGACACATTA AGAAATACTT GGACTGACAC AAGAGCCAGC 
AAGAATTCCG ATAATTCTGC TAACGTTCAT CCTGCAAAGC AGCTGAGTAC CATGAAATAC 
ATGACTGCAC ATCACCATAA ACCTGAGATC ATGCAGCAAG AGCTGGCCAT ACATCCTCAT 
TCTGAACAAA ACAAGAGTAG AAGTATGGAG TCAACATTGG GTTACAGGAA ACCTACCTTA 
AGAAGCATTA CCTCTCCTTT GGTTGCTCAC AGACTAAAAC CAATCAGACA GAAAACCAAA 
AAGGCTGTGG TGAGCATCCT TGATTCAGAG GAGGTGTGTG TGGAGCTTCT AAAAGAGTGT 
ACATCTGAAG GACATGTGAA AGAAGTTCTT CAGATATCTA GTGATGGGAC TACGATCACC 
GTTTATTACC CAAATGATGG AAGAGGCTTT CCTCTTGCTG ATAGACCACC CTTGCCTACT 
GACAACATCA GCAGGTACAG CTTTGACAGT TTACCAGAAA AATACTGGCG GAAATATCAG 
TATGCTTCCA GATTCATTCA GCTTGTAAGG TCCAAAACTC CCAAAATCAC TTACTTTACA 
AGATATGCTA AATGTATTTT GATGGAAAAT TCTCCTGGTG CTGATTTTGA AGTTTGGTTT 
TATGATGGAG CCAAAATACA TAAAACAGAA GATGTAATTC ACATAATTGA AAAAACTGGG 
TTATCTTACA CTTTAAAAAA TGAGAATGAC TTTACCAGCT TGAAAGAGGA AGTAAAAATC 
TATATGGACC ATGCCAATGA GGGTCACCGT ACTTGCTTGG CACTGGAATC TGTAATCTCT 
GAAGAGGAAA AAAGAAGCAG GGGCTCTTCA TTCTTCCCAA TAATTGTAGG AAGAAAACCT 
GGTACTACTA GTTCACCTAA AGCCTTATCA CCACCTCCTG TGGACCCAGG CTACTCAAAG 
GGAGAGCAAG CATCATCAAG CAGATTGAGC GCGAATAGTG CGGCTTTCCC AACACAGACA 
CCAGTACTCA GTCCTTCTGC TGTGACAGTG GAAGGACCTG GCCAAACAGC AGCTACTACA 
GGAACAAGCA TCTCTTCAAG TCTTCCTAAA TCAGCACAGC TTTTGAAATC TGTTTTTGTG 
AAAAATGTTG GTTGGGCTAC ACAGCTAACT AGTGGAGCTG TGTGGGTTCA GTTTAATGAT 
GGGTCACAGT TGGTTGTCCA GGCAGGAGTA TCTTCCATCA GTTATACATC ACCAGACGGT 
CAGACAACTA GGTATGGAGA AAATGAAAAA TTGCCTGAAT ACATCAAACA GAAATTGCAA 
TGTCTTTCTT CCATCCTTCT GATGTTTTCT AATCCAACTC CTAGTTTTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232362.1
CDS	90..2723
Translation	MYKAGMVQRVQNEVKIHCQLKHPSVLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPFSESEARHFMHQIITGMLYLHSHGILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSTKSKDLGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRRLLVGQPLPNKITVFQKNKNSSDFSSGDGSNFCTQWGNPEQEANNRGRGRVIEDAEERPHSRYLRRAHSSDRSNPSNQSRAKTYSIERCHSVEMLSKPRRSSLDETKHSSNHHCLGKTPFPFADQTPQMEIVQQWFGNLQMNGETSEHNTISPNRDFQDYPDVQDTLRNTWTDTRASKNSDNSANVHPAKQLSTMKYMTAHHHKPEIMQQELAIHPHSEQNKSRSMESTLGYRKPTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELLKECTSEGHVKEVLQISSDGTTITVYYPNDGRGFPLADRPPLPTDNISRYSFDSLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPGADFEVWFYDGAKIHKTEDVIHIIEKTGLSYTLKNENDFTSLKEEVKIYMDHANEGHRTCLALESVISEEEKRSRGSSFFPIIVGRKPGTTSSPKALSPPPVDPGYSKGEQASSSRLSANSAAFPTQTPVLSPSAVTVEGPGQTAATTGTSISSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSILLMFSNPTPSFQ

Target ORF information:

RefSeq Version	XM_006232300.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232300.1

ORF Insert Sequence:

ATGTACAAAG CTGGAATGGT ACAGAGAGTC CAAAATGAGG TGAAAATACA TTGCCAGTTG 
AAACACCCCT CTGTCTTGGA GCTCTATAAT TACTTTGAAG ATAACAATTA CGTATACCTG 
GTATTAGAAA TGTGCCACAA CGGAGAAATG AACAGGTACC TAAAGAACAG AATGAAGCCT 
TTCTCCGAAA GCGAAGCCAG GCACTTCATG CACCAGATCA TCACAGGAAT GCTGTATCTT 
CATTCTCACG GCATACTACA CCGCGACCTC ACACTCTCTA ACATCTTACT TACACGGAAT 
ATGAACATAA AAATTGCTGA CTTTGGACTA GCAACACAAT TGAAAATGCC ACATGAGAAG 
CATTACACCC TGTGTGGAAC CCCTAATTAT ATTTCACCAG AAATTGCAAC TCGAAGTGCA 
CATGGACTTG AATCTGATAT TTGGTCATTG GGCTGTATGT TTTATACATT ACTTATTGGA 
AGACCACCTT TTGACACTGA TACAGTCAAA AACACATTGA ACAAAGTAGT ATTGGCAGAT 
TATGAAATGC CAGCCTTTTT GTCACGAGAG GCCCAGGACC TTATCCACCA ATTACTTCGT 
AGAAACCCTG CAGATCGGTT AAGTCTGTCT TCTGTGTTGG ACCATCCTTT TATGTCACGA 
AATCCTTCAA CAAAGAGTAA AGACTTAGGG ACTGTAGAGG ACTCAATGGA TAGTGGGCAT 
GCCACACTTT CCACAACAAT TACAGCTTCT TCCGGAACCA GTTTGAGTGG TAGCCTACTT 
GACAGAAGAA GACTTTTGGT TGGTCAACCA CTTCCAAATA AAATTACTGT ATTTCAAAAA 
AATAAAAATT CAAGTGACTT TTCTTCAGGA GATGGAAGTA ATTTTTGTAC TCAATGGGGA 
AATCCAGAAC AAGAAGCTAA TAATAGGGGG CGGGGAAGAG TAATTGAAGA TGCAGAAGAA 
AGGCCACATT CTCGATACCT TCGCAGAGCT CATTCCTCTG ATAGATCCAA CCCTTCTAAT 
CAGTCTCGAG CAAAAACATA CTCAATAGAG CGGTGTCACT CAGTAGAAAT GCTTTCAAAG 
CCCAGAAGAT CATCACTGGA TGAAACTAAA CACAGTTCCA ATCATCATTG TCTAGGGAAA 
ACTCCTTTTC CATTTGCAGA CCAGACACCT CAGATGGAGA TAGTACAACA GTGGTTTGGG 
AATCTGCAAA TGAATGGAGA AACTAGTGAG CACAACACCA TTAGCCCAAA CAGAGATTTC 
CAGGACTATC CTGATGTGCA GGACACATTA AGAAATACTT GGACTGACAC AAGAGCCAGC 
AAGAATTCCG ATAATTCTGC TAACGTTCAT CCTGCAAAGC AGCTGAGTAC CATGAAATAC 
ATGACTGCAC ATCACCATAA ACCTGAGATC ATGCAGCAAG AGCTGGCCAT ACATCCTCAT 
TCTGAACAAA ACAAGAGTAG AAGTATGGAG TCAACATTGG GTTACAGGAA ACCTACCTTA 
AGAAGCATTA CCTCTCCTTT GGTTGCTCAC AGACTAAAAC CAATCAGACA GAAAACCAAA 
AAGGCTGTGG TGAGCATCCT TGATTCAGAG GAGGTGTGTG TGGAGCTTCT AAAAGAGTGT 
ACATCTGAAG GACATGTGAA AGAAGTTCTT CAGATATCTA GTGATGGGAC TACGATCACC 
GTTTATTACC CAAATGATGG AAGAGGCTTT CCTCTTGCTG ATAGACCACC CTTGCCTACT 
GACAACATCA GCAGGTACAG CTTTGACAGT TTACCAGAAA AATACTGGCG GAAATATCAG 
TATGCTTCCA GATTCATTCA GCTTGTAAGG TCCAAAACTC CCAAAATCAC TTACTTTACA 
AGATATGCTA AATGTATTTT GATGGAAAAT TCTCCTGGTG CTGATTTTGA AGTTTGGTTT 
TATGATGGAG CCAAAATACA TAAAACAGAA GATGTAATTC ACATAATTGA AAAAACTGGG 
TTATCTTACA CTTTAAAAAA TGAGAATGAC TTTACCAGCT TGAAAGAGGA AGTAAAAATC 
TATATGGACC ATGCCAATGA GGGTCACCGT ACTTGCTTGG CACTGGAATC TGTAATCTCT 
GAAGAGGAAA AAAGAAGCAG GGGCTCTTCA TTCTTCCCAA TAATTGTAGG AAGAAAACCT 
GGTACTACTA GTTCACCTAA AGCCTTATCA CCACCTCCTG TGGACCCAGG CTACTCAAAG 
GGAGAGCAAG CATCATCAAG CAGATTGAGC GCGAATAGTG CGGCTTTCCC AACACAGACA 
CCAGTACTCA GTCCTTCTGC TGTGACAGTG GAAGGACCTG GCCAAACAGC AGCTACTACA 
GGAACAAGCA TCTCTTCAAG TCTTCCTAAA TCAGCACAGC TTTTGAAATC TGTTTTTGTG 
AAAAATGTTG GTTGGGCTAC ACAGCTAACT AGTGGAGCTG TGTGGGTTCA GTTTAATGAT 
GGGTCACAGT TGGTTGTCCA GGCAGGAGTA TCTTCCATCA GTTATACATC ACCAGACGGT 
CAGACAACTA GGTATGGAGA AAATGAAAAA TTGCCTGAAT ACATCAAACA GAAATTGCAA 
TGTCTTTCTT CCATCCTTCT GATGTTTTCT AATCCAACTC CTAGTTTTCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plk4 ( XM_017590857.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446346.1 Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59742
Clone ID Related Accession (Same CDS sequence)	XM_017590857.1
Accession Version	XM_017590857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase PLK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCCA CCCCAGACAC CTGCATCATC GAGGACTTTA AGGTTGGAAA TCTACTTGGT 
AAAGGATCAT TTGCTGGTGT CTACAGAGCT GAGTCCATAC ACACTGGTTT GGAAGTTGCA 
ATCAAAATGA TAGATAAGAA AGCCATGTAC AAAGCTGGAA TGGTACAGAG AGTCCAAAAT 
GAGGTGAAAA TACATTGCCA GTTGAAACAC CCCTCTGTCT TGGAGCTCTA TAATTACTTT 
GAAGATAACA ATTACGTATA CCTGGTATTA GAAATGTGCC ACAACGGAGA AATGAACAGG 
TACCTAAAGA ACAGAATGAA GCCTTTCTCC GAAAGCGAAG CCAGGCACTT CATGCACCAG 
ATCATCACAG GAATGCTGTA TCTTCATTCT CACGGCATAC TACACCGCGA CCTCACACTC 
TCTAACATCT TACTTACACG GAATATGAAC ATAAAAATTG CTGACTTTGG ACTAGCAACA 
CAATTGAAAA TGCCACATGA GAAGCATTAC ACCCTGTGTG GAACCCCTAA TTATATTTCA 
CCAGAAATTG CAACTCGAAG TGCACATGGA CTTGAATCTG ATATTTGGTC ATTGGGCTGT 
ATGTTTTATA CATTACTTAT TGGAAGACCA CCTTTTGACA CTGATACAGT CAAAAACACA 
TTGAACAAAG TAGTATTGGC AGATTATGAA ATGCCAGCCT TTTTGTCACG AGAGGCCCAG 
GACCTTATCC ACCAATTACT TCGTAGAAAC CCTGCAGATC GGTTAAGTCT GTCTTCTGTG 
TTGGACCATC CTTTTATGTC ACGAAATCCT TCAACAAAGA GTAAAGACTT AGGGACTGTA 
GAGGACTCAA TGGATAGTGG GCATGCCACA CTTTCCACAA CAATTACAGC TTCTTCCGGA 
ACCAGTTTGA GTGGTAGCCT ACTTGACAGA AGAAGACTTT TGGTTGGTCA ACCACTTCCA 
AATAAAATTA CTGTATTTCA AAAAAATAAA AATTCAAGTG ACTTTTCTTC AGGAGATGGA 
AGTAATTTTT GTACTCAATG GGGAAATCCA GAACAAGAAG CTAATAATAG GGGGCGGGGA 
AGAGTAATTG AAGATGCAGA AGAAAGGCCA CATTCTCGAT ACCTTCGCAG AGCTCATTCC 
TCTGATAGAT CCAACCCTTC TAATCAGTCT CGAGCAAAAA CATACTCAAT AGAGCGGTGT 
CACTCAGTAG AAATGCTTTC AAAGCCCAGA AGATCATCAC TGGATGAAAC TAAACACAGT 
TCCAATCATC ATTGTCTAGG GAAAACTCCT TTTCCATTTG CAGACCAGAC ACCTCAGATG 
GAGATAGTAC AACAGTGGTT TGGGAATCTG CAAATGAATG GAGAAACTAG TGAGCACAAC 
ACCATTAGCC CAAACAGAGA TTTCCAGGAC TATCCTGATG TGCAGGACAC ATTAAGAAAT 
ACTTGGACTG ACACAAGAGC CAGCAAGAAT TCCGATAATT CTGCTAACGT TCATCCTGCA 
AAGCAGCTGA GTACCATGAA ATACATGACT GCACATCACC ATAAACCTGA GATCATGCAG 
CAAGAGCTGG CCATACATCC TCATTCTGAA CAAAACAAGA GTAGAAGTAT GGAGTCAACA 
TTGGGTTACA GGAAACCTAC CTTAAGAAGC ATTACCTCTC CTTTGGTTGC TCACAGACTA 
AAACCAATCA GACAGAAAAC CAAAAAGGCT GTGGTGAGCA TCCTTGATTC AGAGGAGGTG 
TGTGTGGAGC TTCTAAAAGA GTGTACATCT GAAGGACATG TGAAAGAAGT TCTTCAGATA 
TCTAGTGATG GGACTACGAT CACCGTTTAT TACCCAAATG ATGGAAGAGG CTTTCCTCTT 
GCTGATAGAC CACCCTTGCC TACTGACAAC ATCAGCAGGT ACAGCTTTGA CAGTTTACCA 
GAAAAATACT GGCGGAAATA TCAGTATGCT TCCAGATTCA TTCAGCTTGT AAGGTCCAAA 
ACTCCCAAAA TCACTTACTT TACAAGATAT GCTAAATGTA TTTTGATGGA AAATTCTCCT 
GGTGCTGATT TTGAAGTTTG GTTTTATGAT GGAGCCAAAA TACATAAAAC AGAAGATGTA 
ATTCACATAA TTGAAAAAAC TGGGTTATCT TACACTTTAA AAAATGAGAA TGACTTTACC 
AGCTTGAAAG AGGAAGTAAA AATCTATATG GACCATGCCA ATGAGGGTCA CCGTACTTGC 
TTGGCACTGG AATCTGTAAT CTCTGAAGAG GAAAAAAGAA GCAGGGGCTC TTCATTCTTC 
CCAATAATTG TAGGAAGAAA ACCTGGTACT ACTAGTTCAC CTAAAGCCTT ATCACCACCT 
CCTGTGGACC CAGGCTACTC AAAGGGAGAG CAAGCATCAT CAAGCAGATT GAGCGCGAAT 
AGTGCGGCTT TCCCAACACA GACACCAGTA CTCAGTCCTT CTGCTGTGAC AGTGGAAGGA 
CCTGGCCAAA CAGCAGCTAC TACAGGAACA AGCATCTCTT CAAGTCTTCC TAAATCAGCA 
CAGCTTTTGA AATCTGTTTT TGTGAAAAAT GTTGGTTGGG CTACACAGCT AACTAGTGGA 
GCTGTGTGGG TTCAGTTTAA TGATGGGTCA CAGTTGGTTG TCCAGGCAGG AGTATCTTCC 
ATCAGTTATA CATCACCAGA CGGTCAGACA ACTAGGTATG GAGAAAATGA AAAATTGCCT 
GAATACATCA AACAGAAATT GCAATGTCTT TCTTCCATCC TTCTGATGTT TTCTAATCCA 
ACTCCTAGTT TTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446346.1
CDS	513..3290
Translation	MPSTPDTCIIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSVLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPFSESEARHFMHQIITGMLYLHSHGILHRDLTLSNILLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSTKSKDLGTVEDSMDSGHATLSTTITASSGTSLSGSLLDRRRLLVGQPLPNKITVFQKNKNSSDFSSGDGSNFCTQWGNPEQEANNRGRGRVIEDAEERPHSRYLRRAHSSDRSNPSNQSRAKTYSIERCHSVEMLSKPRRSSLDETKHSSNHHCLGKTPFPFADQTPQMEIVQQWFGNLQMNGETSEHNTISPNRDFQDYPDVQDTLRNTWTDTRASKNSDNSANVHPAKQLSTMKYMTAHHHKPEIMQQELAIHPHSEQNKSRSMESTLGYRKPTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELLKECTSEGHVKEVLQISSDGTTITVYYPNDGRGFPLADRPPLPTDNISRYSFDSLPEKYWRKYQYASRFIQLVRSKTPKITYFTRYAKCILMENSPGADFEVWFYDGAKIHKTEDVIHIIEKTGLSYTLKNENDFTSLKEEVKIYMDHANEGHRTCLALESVISEEEKRSRGSSFFPIIVGRKPGTTSSPKALSPPPVDPGYSKGEQASSSRLSANSAAFPTQTPVLSPSAVTVEGPGQTAATTGTSISSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSILLMFSNPTPSFQ

Target ORF information:

RefSeq Version	XM_017590857.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polo-like kinase 4 (Plk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590857.1

ORF Insert Sequence:

ATGCCTTCCA CCCCAGACAC CTGCATCATC GAGGACTTTA AGGTTGGAAA TCTACTTGGT 
AAAGGATCAT TTGCTGGTGT CTACAGAGCT GAGTCCATAC ACACTGGTTT GGAAGTTGCA 
ATCAAAATGA TAGATAAGAA AGCCATGTAC AAAGCTGGAA TGGTACAGAG AGTCCAAAAT 
GAGGTGAAAA TACATTGCCA GTTGAAACAC CCCTCTGTCT TGGAGCTCTA TAATTACTTT 
GAAGATAACA ATTACGTATA CCTGGTATTA GAAATGTGCC ACAACGGAGA AATGAACAGG 
TACCTAAAGA ACAGAATGAA GCCTTTCTCC GAAAGCGAAG CCAGGCACTT CATGCACCAG 
ATCATCACAG GAATGCTGTA TCTTCATTCT CACGGCATAC TACACCGCGA CCTCACACTC 
TCTAACATCT TACTTACACG GAATATGAAC ATAAAAATTG CTGACTTTGG ACTAGCAACA 
CAATTGAAAA TGCCACATGA GAAGCATTAC ACCCTGTGTG GAACCCCTAA TTATATTTCA 
CCAGAAATTG CAACTCGAAG TGCACATGGA CTTGAATCTG ATATTTGGTC ATTGGGCTGT 
ATGTTTTATA CATTACTTAT TGGAAGACCA CCTTTTGACA CTGATACAGT CAAAAACACA 
TTGAACAAAG TAGTATTGGC AGATTATGAA ATGCCAGCCT TTTTGTCACG AGAGGCCCAG 
GACCTTATCC ACCAATTACT TCGTAGAAAC CCTGCAGATC GGTTAAGTCT GTCTTCTGTG 
TTGGACCATC CTTTTATGTC ACGAAATCCT TCAACAAAGA GTAAAGACTT AGGGACTGTA 
GAGGACTCAA TGGATAGTGG GCATGCCACA CTTTCCACAA CAATTACAGC TTCTTCCGGA 
ACCAGTTTGA GTGGTAGCCT ACTTGACAGA AGAAGACTTT TGGTTGGTCA ACCACTTCCA 
AATAAAATTA CTGTATTTCA AAAAAATAAA AATTCAAGTG ACTTTTCTTC AGGAGATGGA 
AGTAATTTTT GTACTCAATG GGGAAATCCA GAACAAGAAG CTAATAATAG GGGGCGGGGA 
AGAGTAATTG AAGATGCAGA AGAAAGGCCA CATTCTCGAT ACCTTCGCAG AGCTCATTCC 
TCTGATAGAT CCAACCCTTC TAATCAGTCT CGAGCAAAAA CATACTCAAT AGAGCGGTGT 
CACTCAGTAG AAATGCTTTC AAAGCCCAGA AGATCATCAC TGGATGAAAC TAAACACAGT 
TCCAATCATC ATTGTCTAGG GAAAACTCCT TTTCCATTTG CAGACCAGAC ACCTCAGATG 
GAGATAGTAC AACAGTGGTT TGGGAATCTG CAAATGAATG GAGAAACTAG TGAGCACAAC 
ACCATTAGCC CAAACAGAGA TTTCCAGGAC TATCCTGATG TGCAGGACAC ATTAAGAAAT 
ACTTGGACTG ACACAAGAGC CAGCAAGAAT TCCGATAATT CTGCTAACGT TCATCCTGCA 
AAGCAGCTGA GTACCATGAA ATACATGACT GCACATCACC ATAAACCTGA GATCATGCAG 
CAAGAGCTGG CCATACATCC TCATTCTGAA CAAAACAAGA GTAGAAGTAT GGAGTCAACA 
TTGGGTTACA GGAAACCTAC CTTAAGAAGC ATTACCTCTC CTTTGGTTGC TCACAGACTA 
AAACCAATCA GACAGAAAAC CAAAAAGGCT GTGGTGAGCA TCCTTGATTC AGAGGAGGTG 
TGTGTGGAGC TTCTAAAAGA GTGTACATCT GAAGGACATG TGAAAGAAGT TCTTCAGATA 
TCTAGTGATG GGACTACGAT CACCGTTTAT TACCCAAATG ATGGAAGAGG CTTTCCTCTT 
GCTGATAGAC CACCCTTGCC TACTGACAAC ATCAGCAGGT ACAGCTTTGA CAGTTTACCA 
GAAAAATACT GGCGGAAATA TCAGTATGCT TCCAGATTCA TTCAGCTTGT AAGGTCCAAA 
ACTCCCAAAA TCACTTACTT TACAAGATAT GCTAAATGTA TTTTGATGGA AAATTCTCCT 
GGTGCTGATT TTGAAGTTTG GTTTTATGAT GGAGCCAAAA TACATAAAAC AGAAGATGTA 
ATTCACATAA TTGAAAAAAC TGGGTTATCT TACACTTTAA AAAATGAGAA TGACTTTACC 
AGCTTGAAAG AGGAAGTAAA AATCTATATG GACCATGCCA ATGAGGGTCA CCGTACTTGC 
TTGGCACTGG AATCTGTAAT CTCTGAAGAG GAAAAAAGAA GCAGGGGCTC TTCATTCTTC 
CCAATAATTG TAGGAAGAAA ACCTGGTACT ACTAGTTCAC CTAAAGCCTT ATCACCACCT 
CCTGTGGACC CAGGCTACTC AAAGGGAGAG CAAGCATCAT CAAGCAGATT GAGCGCGAAT 
AGTGCGGCTT TCCCAACACA GACACCAGTA CTCAGTCCTT CTGCTGTGAC AGTGGAAGGA 
CCTGGCCAAA CAGCAGCTAC TACAGGAACA AGCATCTCTT CAAGTCTTCC TAAATCAGCA 
CAGCTTTTGA AATCTGTTTT TGTGAAAAAT GTTGGTTGGG CTACACAGCT AACTAGTGGA 
GCTGTGTGGG TTCAGTTTAA TGATGGGTCA CAGTTGGTTG TCCAGGCAGG AGTATCTTCC 
ATCAGTTATA CATCACCAGA CGGTCAGACA ACTAGGTATG GAGAAAATGA AAAATTGCCT 
GAATACATCA AACAGAAATT GCAATGTCTT TCTTCCATCC TTCTGATGTT TTCTAATCCA 
ACTCCTAGTT TTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KAT2A/KAT2B-targeted acetylome reveals a role for PLK4 acetylation in preventing centrosome amplification.
Nature communications713227(2016 10)
Fournier M,Orpinell M,Grauffel C,Scheer E,Garnier JM,Ye T,Chavant V,Joint M,Esashi F,Dejaegere A,Gönczy P,Tora L

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis.
Nature structural & molecular biology21(8)696-703(2014 Aug)
Park SY,Park JE,Kim TS,Kim JH,Kwak MJ,Ku B,Tian L,Murugan RN,Ahn M,Komiya S,Hojo H,Kim NH,Kim BY,Bang JK,Erikson RL,Lee KW,Kim SJ,Oh BH,Yang W,Lee KS

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Nature cell biology15(12)1434-44(2013 Dec)
Zhao H,Zhu L,Zhu Y,Cao J,Li S,Huang Q,Xu T,Huang X,Yan X,Zhu X

Polo-like kinase is required for synaptonemal complex disassembly and phosphorylation in mouse spermatocytes.
Journal of cell science125(Pt 21)5061-72(2012 Nov)
Jordan PW,Karppinen J,Handel MA

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Plk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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