Sh3rf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sh3rf1
Entrez Gene ID	306417
Full Name	SH3 domain containing ring finger 1
Synonyms	Posh,Sh3md2
General protein information	Preferred Names SH3 domain containing ring finger 1 Names E3 ubiquitin-protein ligase SH3RF1 RING-type E3 ubiquitin transferase SH3RF1 SH3 domain-containing RING finger protein 1 SH3 multiple domains protein 2 plenty of SH3s putative E3 ubiquitin-protein ligase SH3RF1 putative scaffolding protein POSH
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p12
Summary	putative scaffolding protein; involved in Rac-induced signal transduction pathways [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_198764.1	NP_942059.1	E3 ubiquitin-protein ligase SH3RF1
XM_017600106.1	XP_017455595.1	E3 ubiquitin-protein ligase SH3RF1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH3RF1	NP_065921.2
Xenopus tropicalis (tropical clawed frog)	sh3rf1	NP_001015973.1
Mus musculus (house mouse)	Sh3rf1	NP_067481.2
Rattus norvegicus (Norway rat)	Sh3rf1	NP_942059.1
Macaca mulatta (Rhesus monkey)	SH3RF1	XP_001082524.1
Gallus gallus (chicken)	SH3RF1	XP_420402.2
Danio rerio (zebrafish)	sh3rf1	NP_001038952.2
Pan troglodytes (chimpanzee)	SH3RF1	XP_517530.2
Canis lupus familiaris (dog)	SH3RF1	XP_855736.2
Bos taurus (cattle)	SH3RF1	NP_001091534.1

Gene Ontology
Bibliography

Related articles in PubMed

POSH Regulates CD4+ T Cell Differentiation and Survival.
Cunningham CA, Cardwell LN, Guan Y, Teixeiro E, Daniels MA
Journal of immunology (Baltimore, Md. : 1950)196(10)4003-13(2016 05)

The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.
Cunningham CA, Knudson KM, Peng BJ, Teixeiro E, Daniels MA
European journal of immunology43(12)3361-71(2013 Dec)

POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.
Yang T, Sun Y, Zhang F, Zhu Y, Shi L, Li H, Xu Z
Cell reports2(3)640-51(2012 Sep)

Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.
Wilhelm M, Kukekov NV, Schmit TL, Biagas KV, Sproul AA, Gire S, Maes ME, Xu Z, Greene LA
The Journal of biological chemistry287(3)2247-56(2012 Jan)

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Christian PA, Fiandalo MV, Schwarze SR
Molecular cancer1057(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sh3rf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sh3rf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04996	NM_198764.1 Latest version!	Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa58805	XM_017600106.1 Latest version!	Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sh3rf1 ( NM_198764.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_942059.1 Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.

Price & Availability↑

CloneID	ORa04996
Clone ID Related Accession (Same CDS sequence)	NM_198764.1
Accession Version	NM_198764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SH3RF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF515735.1. On Dec 17, 2003 this sequence version replaced XM_224802.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF515735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCTTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAC ACGTTTTGCA AACGATGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTT 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCTCG ACCACCTGCA CAAATGTATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCAAAA GATCTGCAGA GCCCCCAGTG TGGACAGCAA 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTTTGC GACGACAAGT GGATGAAAAT TGGTACCATG GAGAAGTCAA CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT TGTGCAGATC ATCAAACCTT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAGG CTGACAAAGA TTGCCTTCCC 
TTTGCAAAGG ATGACGTACT GACTGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG GATATTTCCA ATTTCATATG TTGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCG GTACCAGGAG TGGACACTGC AGAATGCCCT 
TCAGCGACAG CGGCGCAGAG CAGCTCTGCC TCAAAGCACT CCGATACCAA GAAGAACACC 
AGGAAGCGAC ACTCCTTCAC CTCCCTCACC ATGGCCAACA AGTCTTCCCA GGCGTCCCAG 
AACCGCCACT CCATGGAGAT CAGCCCTCCT GTGCTTATCA GCTCCAGCAA CCCCACGGCC 
GCAGCCCGCA TCAGCGAGCT CTCCGGGCTC TCCTGCAGCG CCCCGTCTCA GGTTCATATA 
AGCACCACTG GGTTAATTGT GACCCCACCC CCTAGCAGCC CAGTGACAAC TGGCCCTTCG 
TTCACGTTCC CAACCGACGT CCCCTACCAA GCTGCCCTTG GAACGATGAA TCCTCCTCTC 
CCCCCACCCC CTCTCCTGGC CACCACTGTA CTCGCCTCTA CACCGTCAGG CGCTACTGCT 
GCTGCCGTTG CTGCTGCTGC TGCTGCTGTT GCTGCTGGAG TGGGACCCAG GCCTGCAGTG 
GGATCCACGG AACAGATCGC ACATTTACGG CCGCAGACCC GTCCCAGTGT ATATGTTGCT 
ATATATCCAT ACACTCCCCG GAAGGAAGAC GAACTGGAGC TGAGGAAAGG CGAGATGTTT 
TTGGTCTTCG AGCGTTGCCA GGACGGCTGG TACAAAGGAA CGTCAATGCA CACCAGCAAG 
ATCGGCGTTT TCCCCGGCAA CTATGTGGCA CCCGTCACAA GGGCGGTGAC AAATGCCTCC 
CAAGCTAAAG TCCCTATGTC TACAGCGGGT CAGGCAAGTC GTGGGGTGAC TATGGTCAGT 
CCTTCCACCG CAGGAGGGCC TGCACAGAAG CCCCAAGGAA ATGGCGTGGC TGGGAATCCC 
AGCGTCGTCC CCACAGCTGT GGTGTCAGCA GCTCATATCC AGACAAGTCC CCAGGCTAAG 
GTCCTGCTGC ACATGACTGG GCAGATGACA GTCAACCAGG CCCGAAATGC TGTGAGGACA 
GTTGCAGCAC ATAACCAGGA GCGCCCCACA GCAGCAGTGA CTCCCATCCA GGTCCAGAAT 
GCAGCCTGCA TCGGTCCTGC ATCTGTAGGC CTGCCCCATC ATTCTCTGGC CTCCCAACCT 
CTGCCTCCAA TGGTAGGTCC TGCTGCCCAC ATTGCTGCCG TCAACATCAA TCGAACCAGT 
GTCCCCCTGG CCTGCGCTGC AGGGGCATCC TCTCTGGCCT CCCCAAACAT GACCACTGCT 
GCTTTGGAGA CGGAGCCCAG TGGTCGGACA GTGACCATCC TCCCTGGACT CCCCACATCT 
CCTGAGAGTG CTGCATCGGC CTGTGGGAAC AGTTCAGCTG TGAAACCAGA CAAGGACAGT 
AAGAAAGAAA AAAAGGGCCT ATTGAAGCTG CTTTCTGGCG CCTCCACCAA ACGCAAGCCC 
CGAGTCTCCC CTCCAGCATC ACCTACCCTG GACGTGGAGC TGGGTTCAGG GGAGGTTCCC 
TTGCAGGGAG CAGTGGGTCC TGAGTTGCCG CTAGGGGGCG TCCATGGCAG AGTGGGGTCC 
TGCCCCACCG ATGGGGATGG TCCAGTGGCC GCAGGGACAG CAGCCTTAGC CCAGGATGCC 
TTCCATCGCA AGACAAGCTC CCTGGACTCC GCAGTGCCCA TCGCTCCACC ACCTCGCCAG 
GCCTGCTCCT CCCTGGGTCC AGTAATGAAT GAGGCCAGGC CTGTTGTTTG TGAAAGGCAC 
AGAGTGGTGG TTTCCTATCC TCCTCAGAGT GAGGCAGAAC TCGAACTGAA AGAAGGAGAT 
ATTGTGTTTG TTCATAAGAA ACGAGAGGAC GGCTGGTTCA AAGGCACGTT ACAAAGAAAC 
GGGAAGACTG GTCTTTTCCC AGGGAGCTTT GTGGAAAACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942059.1
CDS	1..2685
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLVRLLDGIKQRPWKPGPGGGGSTTCTNVLRAQGSTVVNCGSKDLQSPQCGQQPRVQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVNGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAAQSSSASKHSDTKKNTRKRHSFTSLTMANKSSQASQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPSFTFPTDVPYQAALGTMNPPLPPPPLLATTVLASTPSGATAAAVAAAAAAVAAGVGPRPAVGSTEQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVPMSTAGQASRGVTMVSPSTAGGPAQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAACIGPASVGLPHHSLASQPLPPMVGPAAHIAAVNINRTSVPLACAAGASSLASPNMTTAALETEPSGRTVTILPGLPTSPESAASACGNSSAVKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLDVELGSGEVPLQGAVGPELPLGGVHGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

Target ORF information:

RefSeq Version	NM_198764.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198764.1

ORF Insert Sequence:

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCTTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAC ACGTTTTGCA AACGATGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTT 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCTCG ACCACCTGCA CAAATGTATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCAAAA GATCTGCAGA GCCCCCAGTG TGGACAGCAA 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTTTGC GACGACAAGT GGATGAAAAT TGGTACCATG GAGAAGTCAA CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT TGTGCAGATC ATCAAACCTT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAGG CTGACAAAGA TTGCCTTCCC 
TTTGCAAAGG ATGACGTACT GACTGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG GATATTTCCA ATTTCATATG TTGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCG GTACCAGGAG TGGACACTGC AGAATGCCCT 
TCAGCGACAG CGGCGCAGAG CAGCTCTGCC TCAAAGCACT CCGATACCAA GAAGAACACC 
AGGAAGCGAC ACTCCTTCAC CTCCCTCACC ATGGCCAACA AGTCTTCCCA GGCGTCCCAG 
AACCGCCACT CCATGGAGAT CAGCCCTCCT GTGCTTATCA GCTCCAGCAA CCCCACGGCC 
GCAGCCCGCA TCAGCGAGCT CTCCGGGCTC TCCTGCAGCG CCCCGTCTCA GGTTCATATA 
AGCACCACTG GGTTAATTGT GACCCCACCC CCTAGCAGCC CAGTGACAAC TGGCCCTTCG 
TTCACGTTCC CAACCGACGT CCCCTACCAA GCTGCCCTTG GAACGATGAA TCCTCCTCTC 
CCCCCACCCC CTCTCCTGGC CACCACTGTA CTCGCCTCTA CACCGTCAGG CGCTACTGCT 
GCTGCCGTTG CTGCTGCTGC TGCTGCTGTT GCTGCTGGAG TGGGACCCAG GCCTGCAGTG 
GGATCCACGG AACAGATCGC ACATTTACGG CCGCAGACCC GTCCCAGTGT ATATGTTGCT 
ATATATCCAT ACACTCCCCG GAAGGAAGAC GAACTGGAGC TGAGGAAAGG CGAGATGTTT 
TTGGTCTTCG AGCGTTGCCA GGACGGCTGG TACAAAGGAA CGTCAATGCA CACCAGCAAG 
ATCGGCGTTT TCCCCGGCAA CTATGTGGCA CCCGTCACAA GGGCGGTGAC AAATGCCTCC 
CAAGCTAAAG TCCCTATGTC TACAGCGGGT CAGGCAAGTC GTGGGGTGAC TATGGTCAGT 
CCTTCCACCG CAGGAGGGCC TGCACAGAAG CCCCAAGGAA ATGGCGTGGC TGGGAATCCC 
AGCGTCGTCC CCACAGCTGT GGTGTCAGCA GCTCATATCC AGACAAGTCC CCAGGCTAAG 
GTCCTGCTGC ACATGACTGG GCAGATGACA GTCAACCAGG CCCGAAATGC TGTGAGGACA 
GTTGCAGCAC ATAACCAGGA GCGCCCCACA GCAGCAGTGA CTCCCATCCA GGTCCAGAAT 
GCAGCCTGCA TCGGTCCTGC ATCTGTAGGC CTGCCCCATC ATTCTCTGGC CTCCCAACCT 
CTGCCTCCAA TGGTAGGTCC TGCTGCCCAC ATTGCTGCCG TCAACATCAA TCGAACCAGT 
GTCCCCCTGG CCTGCGCTGC AGGGGCATCC TCTCTGGCCT CCCCAAACAT GACCACTGCT 
GCTTTGGAGA CGGAGCCCAG TGGTCGGACA GTGACCATCC TCCCTGGACT CCCCACATCT 
CCTGAGAGTG CTGCATCGGC CTGTGGGAAC AGTTCAGCTG TGAAACCAGA CAAGGACAGT 
AAGAAAGAAA AAAAGGGCCT ATTGAAGCTG CTTTCTGGCG CCTCCACCAA ACGCAAGCCC 
CGAGTCTCCC CTCCAGCATC ACCTACCCTG GACGTGGAGC TGGGTTCAGG GGAGGTTCCC 
TTGCAGGGAG CAGTGGGTCC TGAGTTGCCG CTAGGGGGCG TCCATGGCAG AGTGGGGTCC 
TGCCCCACCG ATGGGGATGG TCCAGTGGCC GCAGGGACAG CAGCCTTAGC CCAGGATGCC 
TTCCATCGCA AGACAAGCTC CCTGGACTCC GCAGTGCCCA TCGCTCCACC ACCTCGCCAG 
GCCTGCTCCT CCCTGGGTCC AGTAATGAAT GAGGCCAGGC CTGTTGTTTG TGAAAGGCAC 
AGAGTGGTGG TTTCCTATCC TCCTCAGAGT GAGGCAGAAC TCGAACTGAA AGAAGGAGAT 
ATTGTGTTTG TTCATAAGAA ACGAGAGGAC GGCTGGTTCA AAGGCACGTT ACAAAGAAAC 
GGGAAGACTG GTCTTTTCCC AGGGAGCTTT GTGGAAAACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sh3rf1 ( XM_017600106.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455595.1 Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58805
Clone ID Related Accession (Same CDS sequence)	XM_017600106.1
Accession Version	XM_017600106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SH3RF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCTTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAC ACGTTTTGCA AACGATGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTT 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCTCG ACCACCTGCA CAAATGTATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCAAAA GATCTGCAGA GCCCCCAGTG TGGACAGCAA 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTTTGC GACGACAAGT GGATGAAAAT TGGTACCATG GAGAAGTCAA CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT TGTGCAGATC ATCAAACCTT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTTGCAAAGG ATGACGTACT GACTGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG GATATTTCCA ATTTCATATG TTGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCG GTACCAGGAG TGGACACTGC AGAATGCCCT 
TCAGCGACAG CGGCGCAGAG CAGCTCTGCC TCAAAGCACT CCGATACCAA GAAGAACACC 
AGGAAGCGAC ACTCCTTCAC CTCCCTCACC ATGGCCAACA AGTCTTCCCA GGCGTCCCAG 
AACCGCCACT CCATGGAGAT CAGCCCTCCT GTGCTTATCA GCTCCAGCAA CCCCACGGCC 
GCAGCCCGCA TCAGCGAGCT CTCCGGGCTC TCCTGCAGCG CCCCGTCTCA GGTTCATATA 
AGCACCACTG GGTTAATTGT GACCCCACCC CCTAGCAGCC CAGTGACAAC TGGCCCTTCG 
TTCACGTTCC CAACCGACGT CCCCTACCAA GCTGCCCTTG GAACGATGAA TCCTCCTCTC 
CCCCCACCCC CTCTCCTGGC CACCACTGTA CTCGCCTCTA CACCGTCAGG CGCTACTGCT 
GCTGCCGTTG CTGCTGCTGC TGCTGCTGTT GCTGCTGGAG TGGGACCCAG GCCTGCAGTG 
GGATCCACGG AACAGATCGC ACATTTACGG CCGCAGACCC GTCCCAGTGT ATATGTTGCT 
ATATATCCAT ACACTCCCCG GAAGGAAGAC GAACTGGAGC TGAGGAAAGG CGAGATGTTT 
TTGGTCTTCG AGCGTTGCCA GGACGGCTGG TACAAAGGAA CGTCAATGCA CACCAGCAAG 
ATCGGCGTTT TCCCCGGCAA CTATGTGGCA CCCGTCACAA GGGCGGTGAC AAATGCCTCC 
CAAGCTAAAG TCCCTATGTC TACAGCGGGT CAGGCAAGTC GTGGGGTGAC TATGGTCAGT 
CCTTCCACCG CAGGAGGGCC TGCACAGAAG CCCCAAGGAA ATGGCGTGGC TGGGAATCCC 
AGCGTCGTCC CCACAGCTGT GGTGTCAGCA GCTCATATCC AGACAAGTCC CCAGGCTAAG 
GTCCTGCTGC ACATGACTGG GCAGATGACA GTCAACCAGG CCCGAAATGC TGTGAGGACA 
GTTGCAGCAC ATAACCAGGA GCGCCCCACA GCAGCAGTGA CTCCCATCCA GGTCCAGAAT 
GCAGCCTGCA TCGGTCCTGC ATCTGTAGGC CTGCCCCATC ATTCTCTGGC CTCCCAACCT 
CTGCCTCCAA TGGTAGGTCC TGCTGCCCAC ATTGCTGCCG TCAACATCAA TCGAACCAGT 
GTCCCCCTGG CCTGCGCTGC AGGGGCATCC TCTCTGGCCT CCCCAAACAT GACCACTGCT 
GCTTTGGAGA CGGAGCCCAG TGGTCGGACA GTGACCATCC TCCCTGGACT CCCCACATCT 
CCTGAGAGTG CTGCATCGGC CTGTGGGAAC AGTTCAGCTG TGAAACCAGA CAAGGACAGT 
AAGAAAGAAA AAAAGGGCCT ATTGAAGCTG CTTTCTGGCG CCTCCACCAA ACGCAAGCCC 
CGAGTCTCCC CTCCAGCATC ACCTACCCTG GACGTGGAGC TGGGTTCAGG GGAGGTTCCC 
TTGCAGGGAG CAGTGGGTCC TGAGTTGCCG CTAGGGGGCG TCCATGGCAG AGTGGGGTCC 
TGCCCCACCG ATGGGGATGG TCCAGTGGCC GCAGGGACAG CAGCCTTAGC CCAGGATGCC 
TTCCATCGCA AGACAAGCTC CCTGGACTCC GCAGTGCCCA TCGCTCCACC ACCTCGCCAG 
GCCTGCTCCT CCCTGGGTCC AGTAATGAAT GAGGCCAGGC CTGTTGTTTG TGAAAGGCAC 
AGAGTGGTGG TTTCCTATCC TCCTCAGAGT GAGGCAGAAC TTGAACTGAA AGAAGGAGAT 
ATTGTGTTTG TTCATAAGAA ACGAGAGGAC GGCTGGTTCA AAGGCACGTT ACAAAGAAAC 
GGGAAGACTG GTCTTTTCCC AGGGAGCTTT GTGGAGAACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455595.1
CDS	224..2908
Translation	MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLVRLLDGIKQRPWKPGPGGGGSTTCTNVLRAQGSTVVNCGSKDLQSPQCGQQPRVQAWSPPVRGIPQLPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYHGEVNGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEGMLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAAQSSSASKHSDTKKNTRKRHSFTSLTMANKSSQASQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPSFTFPTDVPYQAALGTMNPPLPPPPLLATTVLASTPSGATAAAVAAAAAAVAAGVGPRPAVGSTEQIAHLRPQTRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQAKVPMSTAGQASRGVTMVSPSTAGGPAQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAACIGPASVGLPHHSLASQPLPPMVGPAAHIAAVNINRTSVPLACAAGASSLASPNMTTAALETEPSGRTVTILPGLPTSPESAASACGNSSAVKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLDVELGSGEVPLQGAVGPELPLGGVHGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

Target ORF information:

RefSeq Version	XM_017600106.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SH3 domain containing ring finger 1 (Sh3rf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600106.1

ORF Insert Sequence:

ATGGATGAGT CTGCCTTGTT GGACCTTCTG GAGTGCCCTG TGTGTCTAGA ACGCTTGGAT 
GCTTCCGCAA AGGTCTTACC CTGCCAGCAC ACGTTTTGCA AACGATGTTT GCTGGGGATT 
GTGGGTTCCC GGAATGAACT CAGATGTCCC GAATGCCGGA CTCTTGTTGG CTCTGGGGTT 
GACGAGCTCC CCAGTAACAT CCTACTGGTC AGACTTCTGG ATGGCATCAA GCAGAGGCCT 
TGGAAACCCG GCCCTGGTGG GGGCGGCTCG ACCACCTGCA CAAATGTATT AAGGGCGCAG 
GGCAGCACTG TGGTTAATTG TGGCTCAAAA GATCTGCAGA GCCCCCAGTG TGGACAGCAA 
CCTCGGGTGC AAGCCTGGAG CCCCCCAGTG AGGGGAATAC CTCAGTTACC GTGTGCCAAA 
GCATTATATA ACTACGAAGG AAAAGAGCCC GGAGACCTTA AGTTCAGCAA AGGCGACATC 
ATCATTTTGC GACGACAAGT GGATGAAAAT TGGTACCATG GAGAAGTCAA CGGGGTCCAC 
GGCTTTTTCC CCACCAACTT TGTGCAGATC ATCAAACCTT TACCTCAGCC CCCACCTCAG 
TGCAAAGCAC TTTATGACTT TGAAGTGAAA GACAAGGAAG CTGACAAAGA TTGCCTTCCC 
TTTGCAAAGG ATGACGTACT GACTGTGATC CGCAGAGTGG ATGAAAACTG GGCTGAAGGA 
ATGCTGGCAG ATAAAATAGG GATATTTCCA ATTTCATATG TTGAGTTTAA CTCAGCTGCC 
AAGCAGCTGA TAGAGTGGGA TAAGCCTCCG GTACCAGGAG TGGACACTGC AGAATGCCCT 
TCAGCGACAG CGGCGCAGAG CAGCTCTGCC TCAAAGCACT CCGATACCAA GAAGAACACC 
AGGAAGCGAC ACTCCTTCAC CTCCCTCACC ATGGCCAACA AGTCTTCCCA GGCGTCCCAG 
AACCGCCACT CCATGGAGAT CAGCCCTCCT GTGCTTATCA GCTCCAGCAA CCCCACGGCC 
GCAGCCCGCA TCAGCGAGCT CTCCGGGCTC TCCTGCAGCG CCCCGTCTCA GGTTCATATA 
AGCACCACTG GGTTAATTGT GACCCCACCC CCTAGCAGCC CAGTGACAAC TGGCCCTTCG 
TTCACGTTCC CAACCGACGT CCCCTACCAA GCTGCCCTTG GAACGATGAA TCCTCCTCTC 
CCCCCACCCC CTCTCCTGGC CACCACTGTA CTCGCCTCTA CACCGTCAGG CGCTACTGCT 
GCTGCCGTTG CTGCTGCTGC TGCTGCTGTT GCTGCTGGAG TGGGACCCAG GCCTGCAGTG 
GGATCCACGG AACAGATCGC ACATTTACGG CCGCAGACCC GTCCCAGTGT ATATGTTGCT 
ATATATCCAT ACACTCCCCG GAAGGAAGAC GAACTGGAGC TGAGGAAAGG CGAGATGTTT 
TTGGTCTTCG AGCGTTGCCA GGACGGCTGG TACAAAGGAA CGTCAATGCA CACCAGCAAG 
ATCGGCGTTT TCCCCGGCAA CTATGTGGCA CCCGTCACAA GGGCGGTGAC AAATGCCTCC 
CAAGCTAAAG TCCCTATGTC TACAGCGGGT CAGGCAAGTC GTGGGGTGAC TATGGTCAGT 
CCTTCCACCG CAGGAGGGCC TGCACAGAAG CCCCAAGGAA ATGGCGTGGC TGGGAATCCC 
AGCGTCGTCC CCACAGCTGT GGTGTCAGCA GCTCATATCC AGACAAGTCC CCAGGCTAAG 
GTCCTGCTGC ACATGACTGG GCAGATGACA GTCAACCAGG CCCGAAATGC TGTGAGGACA 
GTTGCAGCAC ATAACCAGGA GCGCCCCACA GCAGCAGTGA CTCCCATCCA GGTCCAGAAT 
GCAGCCTGCA TCGGTCCTGC ATCTGTAGGC CTGCCCCATC ATTCTCTGGC CTCCCAACCT 
CTGCCTCCAA TGGTAGGTCC TGCTGCCCAC ATTGCTGCCG TCAACATCAA TCGAACCAGT 
GTCCCCCTGG CCTGCGCTGC AGGGGCATCC TCTCTGGCCT CCCCAAACAT GACCACTGCT 
GCTTTGGAGA CGGAGCCCAG TGGTCGGACA GTGACCATCC TCCCTGGACT CCCCACATCT 
CCTGAGAGTG CTGCATCGGC CTGTGGGAAC AGTTCAGCTG TGAAACCAGA CAAGGACAGT 
AAGAAAGAAA AAAAGGGCCT ATTGAAGCTG CTTTCTGGCG CCTCCACCAA ACGCAAGCCC 
CGAGTCTCCC CTCCAGCATC ACCTACCCTG GACGTGGAGC TGGGTTCAGG GGAGGTTCCC 
TTGCAGGGAG CAGTGGGTCC TGAGTTGCCG CTAGGGGGCG TCCATGGCAG AGTGGGGTCC 
TGCCCCACCG ATGGGGATGG TCCAGTGGCC GCAGGGACAG CAGCCTTAGC CCAGGATGCC 
TTCCATCGCA AGACAAGCTC CCTGGACTCC GCAGTGCCCA TCGCTCCACC ACCTCGCCAG 
GCCTGCTCCT CCCTGGGTCC AGTAATGAAT GAGGCCAGGC CTGTTGTTTG TGAAAGGCAC 
AGAGTGGTGG TTTCCTATCC TCCTCAGAGT GAGGCAGAAC TTGAACTGAA AGAAGGAGAT 
ATTGTGTTTG TTCATAAGAA ACGAGAGGAC GGCTGGTTCA AAGGCACGTT ACAAAGAAAC 
GGGAAGACTG GTCTTTTCCC AGGGAGCTTT GTGGAGAACA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POSH Regulates CD4+ T Cell Differentiation and Survival.
Journal of immunology (Baltimore, Md. : 1950)196(10)4003-13(2016 05)
Cunningham CA,Cardwell LN,Guan Y,Teixeiro E,Daniels MA

The POSH/JIP-1 scaffold network regulates TCR-mediated JNK1 signals and effector function in CD8(+) T cells.
European journal of immunology43(12)3361-71(2013 Dec)
Cunningham CA,Knudson KM,Peng BJ,Teixeiro E,Daniels MA

POSH localizes activated Rac1 to control the formation of cytoplasmic dilation of the leading process and neuronal migration.
Cell reports2(3)640-51(2012 Sep)
Yang T,Sun Y,Zhang F,Zhu Y,Shi L,Li H,Xu Z

Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.
The Journal of biological chemistry287(3)2247-56(2012 Jan)
Wilhelm M,Kukekov NV,Schmit TL,Biagas KV,Sproul AA,Gire S,Maes ME,Xu Z,Greene LA

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Molecular cancer1057(2011 May)
Christian PA,Fiandalo MV,Schwarze SR

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Sh3rf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

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