Dars2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dars2
Entrez Gene ID	304919
Full Name	aspartyl-tRNA synthetase 2 (mitochondrial)
Synonyms	RGD1308286
General protein information	Preferred Names aspartyl-tRNA synthetase 2 (mitochondrial) Names aspartate--tRNA ligase, mitochondrial aspRS aspartyl-tRNA synthetase, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034143.1	NP_001029315.1	aspartate--tRNA ligase, mitochondrial precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DARS2	XP_422251.3
Drosophila melanogaster (fruit fly)	CG31739	NP_001260519.1
Schizosaccharomyces pombe (fission yeast)	SPCC736.06	NP_587777.1
Xenopus tropicalis (tropical clawed frog)	dars2	XP_002931512.2
Homo sapiens (human)	DARS2	NP_060592.2
Pan troglodytes (chimpanzee)	DARS2	XP_524974.2
Kluyveromyces lactis	KLLA0E24465g	XP_455053.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007844	XP_317655.3
Neurospora crassa	NCU02585	XP_965716.2
Eremothecium gossypii	AGOS_AGR206C	NP_986872.1
Caenorhabditis elegans (roundworm)	dars-2	NP_506019.2
Macaca mulatta (Rhesus monkey)	DARS2	XP_001101931.2
Danio rerio (zebrafish)	dars2	XP_699804.3
Magnaporthe oryzae (rice blast fungus)	MGG_13512	XP_003716295.1
Canis lupus familiaris (dog)	DARS2	XP_547457.2
Oryza sativa (rice)	Os01g0153400	NP_001042054.1
Bos taurus (cattle)	DARS2	NP_001095692.1
Mus musculus (house mouse)	Dars2	NP_766232.1
Rattus norvegicus (Norway rat)	Dars2	NP_001029315.1
Saccharomyces cerevisiae (baker's yeast)	MSD1	NP_015221.1
Arabidopsis thaliana (thale cress)	AT4G33760	NP_195102.2

Gene Ontology
Bibliography

Related articles in PubMed

Thermodynamic properties distinguish human mitochondrial aspartyl-tRNA synthetase from bacterial homolog with same 3D architecture.
Neuenfeldt A, Lorber B, Ennifar E, Gaudry A, Sauter C, Sissler M, Florentz C
Nucleic acids research41(4)2698-708(2013 Feb)

Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.
Scheper GC, van der Klok T, van Andel RJ, van Berkel CG, Sissler M, Smet J, Muravina TI, Serkov SV, Uziel G, Bugiani M, Schiffmann R, Krägeloh-Mann I, Smeitink JA, Florentz C, Van Coster R, Pronk JC, van der Knaap MS
Nature genetics39(4)534-9(2007 Apr)

Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Giegé R, Florentz C, Sissler M
Biochemistry44(12)4805-16(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12441	NM_001034143.1 Latest version!	Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dars2 ( NM_001034143.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029315.1 Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

Price & Availability↑

CloneID	ORa12441
Clone ID Related Accession (Same CDS sequence)	NM_001034143.1
Accession Version	NM_001034143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Rattus norvegicus(Norway rat)
Product	aspartate--tRNA ligase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105774.1. On Oct 14, 2005 this sequence version replaced XM_222804.3. ##Evidence-Data-START## Transcript exon combination :: BC105774.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTATCTTG GTTCTTGGTT AAATCGTCTA GGCAGGGGTT TATCCAGACC CATCGGAAAG 
ACCAAGCAGC CAATCTGGGG TTCTCTTTCC AGAAGTCTGA CGTTGAGTTC ACAGAGAGTT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAACTTCG TTCTTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GAAGGCAAAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG TCTCGTTCAA ATCCTCATTC CCCAGGATGA GTCGGCTGCA 
TCTGTGAGGA GGACTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACG 
GTCATCGCCC GACCCCTGGG ACAGGAGAAT CCGAAAATGC CAACAGGTGA GATTGAAATC 
AAAGCTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA AATTAAAGAC 
TTTGTGAAGA AGACGGAAGC CCTTCGTCTG CAGTATCGGT ACTTAGACCT GCGCAGCTCC 
CAAATGCAGC ACAACCTGCG GCTCAGGTCA CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTACTCTGC ACGGATTTGT GGATATAGAA ACCCCAACAT TGTTTAAGAG GACTCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCGTCCAGG GAACCTGGAA GGTTTTATTC TCTCCCTCAG 
AGTCCTCAAC AGTTTAAGCA GCTTCTGATG GTTGGTGGTT TAGACAGGTA CTTTCAGGTT 
GCCCGGTGTT ATCGAGATGA AGGCTCAAGA CCGGATAGGC AGCCAGAGTT TACTCAGATT 
GACATAGAGA TGTCCTTTGT AGACCAGACT GGCATTCAGC ATTTAGTTGA GGGTTTGCTG 
CATTATTCCT GGCCTGAAGA TAAAGACCCT CTAGTGGCTC CTTTTCCTTC CATGACTTTT 
GCTGAGGCAT TGGCTACCTA CGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATATCA GTGATGTGTT CAGAAACACA GAGATCAGGT TTCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGAAG GAGCAAAGTA CTTAAGGAAA 
GAAGACATTG AGTTCATTCG AAAGTTTGCA GCTCACCATT TCAGTCAGGA AGTCCTTCCT 
ATATTCCTGA ATGCCAGGAA GAACTGGAGT TCACCATTTG CTAAATTCAT AACGGAGGAG 
GAAAGATTGG AGTTAACCAG ATTGATGGAG ATACAAGAAG ACGACATGGT GCTGCTGACT 
GCTGGGCAAC ATGAGAAAGC ATGCTCTTTG TTGGGGAAGT TACGACTAGA GTGTGCAGAC 
CTTTTGGAAA CGAGAGGACT GGCGCTCAGG GACCCAGCAT TGTTCTCTTT TCTCTGGGTA 
CTTGACTTCC CACTCTTCCT CGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAC 
CACCCATTTA CTGCTCCTCA CCCCGGCGAC ATTCACCTCC TCTACACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTACGA CTTGGTTTTA AATGGCAATG AGATAGGAGG AGGCTCTATC 
CGCATTCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CATTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA TGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTGTGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAT GACCTTATGA GCAATGCTCC AGACACTGTG 
TCTCCTGAAG ATCTGAAGCC TTACCATATC CACGTCTCAT GGCCAACAGA CTCAGAAGAG 
AGAGCATCCG CCACACCTTC CAAATACTTG AGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029315.1
CDS	108..2066
Translation	MYLGSWLNRLGRGLSRPIGKTKQPIWGSLSRSLTLSSQRVPEFSSFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRTLCEAPVESVVRVSGTVIARPLGQENPKMPTGEIEIKAKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSSQMQHNLRLRSQMVMKMREYLCTLHGFVDIETPTLFKRTPGGAKEFLVPSREPGRFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQHLVEGLLHYSWPEDKDPLVAPFPSMTFAEALATYGTDKPDTRFGMKIVDISDVFRNTEIRFLQDALAKPQGTVKAICVHEGAKYLRKEDIEFIRKFAAHHFSQEVLPIFLNARKNWSSPFAKFITEEERLELTRLMEIQEDDMVLLTAGQHEKACSLLGKLRLECADLLETRGLALRDPALFSFLWVLDFPLFLAKEESPTELESAHHPFTAPHPGDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSIRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGHDLMSNAPDTVSPEDLKPYHIHVSWPTDSEERASATPSKYLSS

Target ORF information:

RefSeq Version	NM_001034143.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034143.1

ORF Insert Sequence:

ATGTATCTTG GTTCTTGGTT AAATCGTCTA GGCAGGGGTT TATCCAGACC CATCGGAAAG 
ACCAAGCAGC CAATCTGGGG TTCTCTTTCC AGAAGTCTGA CGTTGAGTTC ACAGAGAGTT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAACTTCG TTCTTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GAAGGCAAAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG TCTCGTTCAA ATCCTCATTC CCCAGGATGA GTCGGCTGCA 
TCTGTGAGGA GGACTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACG 
GTCATCGCCC GACCCCTGGG ACAGGAGAAT CCGAAAATGC CAACAGGTGA GATTGAAATC 
AAAGCTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA AATTAAAGAC 
TTTGTGAAGA AGACGGAAGC CCTTCGTCTG CAGTATCGGT ACTTAGACCT GCGCAGCTCC 
CAAATGCAGC ACAACCTGCG GCTCAGGTCA CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTACTCTGC ACGGATTTGT GGATATAGAA ACCCCAACAT TGTTTAAGAG GACTCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCGTCCAGG GAACCTGGAA GGTTTTATTC TCTCCCTCAG 
AGTCCTCAAC AGTTTAAGCA GCTTCTGATG GTTGGTGGTT TAGACAGGTA CTTTCAGGTT 
GCCCGGTGTT ATCGAGATGA AGGCTCAAGA CCGGATAGGC AGCCAGAGTT TACTCAGATT 
GACATAGAGA TGTCCTTTGT AGACCAGACT GGCATTCAGC ATTTAGTTGA GGGTTTGCTG 
CATTATTCCT GGCCTGAAGA TAAAGACCCT CTAGTGGCTC CTTTTCCTTC CATGACTTTT 
GCTGAGGCAT TGGCTACCTA CGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATATCA GTGATGTGTT CAGAAACACA GAGATCAGGT TTCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGAAG GAGCAAAGTA CTTAAGGAAA 
GAAGACATTG AGTTCATTCG AAAGTTTGCA GCTCACCATT TCAGTCAGGA AGTCCTTCCT 
ATATTCCTGA ATGCCAGGAA GAACTGGAGT TCACCATTTG CTAAATTCAT AACGGAGGAG 
GAAAGATTGG AGTTAACCAG ATTGATGGAG ATACAAGAAG ACGACATGGT GCTGCTGACT 
GCTGGGCAAC ATGAGAAAGC ATGCTCTTTG TTGGGGAAGT TACGACTAGA GTGTGCAGAC 
CTTTTGGAAA CGAGAGGACT GGCGCTCAGG GACCCAGCAT TGTTCTCTTT TCTCTGGGTA 
CTTGACTTCC CACTCTTCCT CGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAC 
CACCCATTTA CTGCTCCTCA CCCCGGCGAC ATTCACCTCC TCTACACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTACGA CTTGGTTTTA AATGGCAATG AGATAGGAGG AGGCTCTATC 
CGCATTCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CATTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA TGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTGTGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAT GACCTTATGA GCAATGCTCC AGACACTGTG 
TCTCCTGAAG ATCTGAAGCC TTACCATATC CACGTCTCAT GGCCAACAGA CTCAGAAGAG 
AGAGCATCCG CCACACCTTC CAAATACTTG AGCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thermodynamic properties distinguish human mitochondrial aspartyl-tRNA synthetase from bacterial homolog with same 3D architecture.
Nucleic acids research41(4)2698-708(2013 Feb)
Neuenfeldt A,Lorber B,Ennifar E,Gaudry A,Sauter C,Sissler M,Florentz C

Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.
Nature genetics39(4)534-9(2007 Apr)
Scheper GC,van der Klok T,van Andel RJ,van Berkel CG,Sissler M,Smet J,Muravina TI,Serkov SV,Uziel G,Bugiani M,Schiffmann R,Krägeloh-Mann I,Smeitink JA,Florentz C,Van Coster R,Pronk JC,van der Knaap MS

Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Biochemistry44(12)4805-16(2005 Mar)
Bonnefond L,Fender A,Rudinger-Thirion J,Giegé R,Florentz C,Sissler M

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Dars2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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