AspRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AspRS-m
Entrez Gene ID	326155
Full Name	Aspartyl-tRNA synthetase, mitochondrial
Synonyms	CG17938,CG31739,Dmel\CG31739
General protein information	Preferred Names Aspartyl-tRNA synthetase, mitochondrial Names CG31739 gene product from transcript CG31739-RA AspRS-m-PA AspRS-m-PB AspRS-m-PC CG31739-PA CG31739-PB CG31739-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 36B2-36B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_165196.2	NP_724018.1	Aspartyl-tRNA synthetase, mitochondrial, isoform A
NM_001273590.1	NP_001260519.1	Aspartyl-tRNA synthetase, mitochondrial, isoform B
NM_001273591.1	NP_001260520.1	Aspartyl-tRNA synthetase, mitochondrial, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DARS2	XP_422251.3
Drosophila melanogaster (fruit fly)	CG31739	NP_001260519.1
Schizosaccharomyces pombe (fission yeast)	SPCC736.06	NP_587777.1
Xenopus tropicalis (tropical clawed frog)	dars2	XP_002931512.2
Homo sapiens (human)	DARS2	NP_060592.2
Pan troglodytes (chimpanzee)	DARS2	XP_524974.2
Kluyveromyces lactis	KLLA0E24465g	XP_455053.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007844	XP_317655.3
Neurospora crassa	NCU02585	XP_965716.2
Eremothecium gossypii	AGOS_AGR206C	NP_986872.1
Caenorhabditis elegans (roundworm)	dars-2	NP_506019.2
Macaca mulatta (Rhesus monkey)	DARS2	XP_001101931.2
Danio rerio (zebrafish)	dars2	XP_699804.3
Magnaporthe oryzae (rice blast fungus)	MGG_13512	XP_003716295.1
Canis lupus familiaris (dog)	DARS2	XP_547457.2
Oryza sativa (rice)	Os01g0153400	NP_001042054.1
Bos taurus (cattle)	DARS2	NP_001095692.1
Mus musculus (house mouse)	Dars2	NP_766232.1
Rattus norvegicus (Norway rat)	Dars2	NP_001029315.1
Saccharomyces cerevisiae (baker's yeast)	MSD1	NP_015221.1
Arabidopsis thaliana (thale cress)	AT4G33760	NP_195102.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AspRS-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AspRS-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15480	NM_001273591.1 Latest version!	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa15480	NM_165196.2 Latest version!	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa15480	NM_001273590.1 Latest version!	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AspRS-m ( NM_165196.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724018.1 Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15480
Clone ID Related Accession (Same CDS sequence)	NM_165196.2 , NM_001273590.1 , NM_001273591.1
Accession Version	NM_165196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Aspartyl-tRNA synthetase, mitochondrial, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165196.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724018.1
CDS	324..3572
Translation	MSGRYRGGGGGRFGGRGGGGYADGGGYNDFDNYRGGGGPGFNDNFGPGPGPNPFNMGGPNISGNELMQLMSAMASNFNMNSQPPQPMRQSCGELRNHHCGLFVELSGRLIKKRVNRFAELRDRNGGACQLVVLEDKHPRVARRMNNMPENTTLTIVGLVMRRPHNSCNQTMPTGEIEVEVQDILNIHFPAGGTKRAGDKRTYSTMVQQQSNLGITSTEYKIAKNENILKYFENRDLTCNDLRRDDVGKTVTLVGWIPSTKNNKFLQLKDGYGQTQLMIEDQSLSDTFLSTPEQTVIQIVGKVLGRPKANVNLKYDTGEVEVSVTSVKVLNPDDPYDGPIKAKEKKQKFSIDDLEAEEAAAEASANLTSNNGESTEDSAEASTTANASLIAEQRAKVADTNKFADRTHNCGELTSNDINEKVVICGWLEFQRMNKFFILRDAYGQTQVLLSPKTYGLEEYAETGVPIESIVRVEGTVIPRPAATINPKMQTGHVEVEADKVVVLNPAKKNLPFEIRKFNRAGERLRLTHRYLDLRFNDMQHNLRLRSAVIMKMREYLINYLGFVEVETPTLFRRTPGGAQEFVVPTRKAGHFYSLVQSPQQFKQMLMSGGIDRYFQVARCYRDEATRPDRQPEFTQLDIELSFTSRDDIMQLIEETLRYSWPKDFPRLQTPFRRITYEEAMEKYGNDKPDTRFGFLLNNVSEIIEKSDEFKEKYDDLGAYAIVVRASEAFWNGAARKHYESLGKEFKGTLFVRKFGPTKDVQEKLGKLLGEDVATEVADKFDLEENDLLFLGIGSKVETRELLGRIRLDYQDFLVENAKIKKPNDFRFLWVIDFPLFERNRETNQLESVHHPFTLPHSDDLENFATSCENLESIRSQAYDLVLNGQEVGGGSIRIHDRDMQHFILEQILKIPHDHLSHLLSALESGCPPHGGIALGLDRLIAILCRARSIRDVIAFPKSLNGRDPLSNAPVPISDEEMRLYHLSVVDEEEPSTNNTSHEQEDDDPDQERAPPSPMSATSESEQPQINVDIKQEPEGLNEDFAPESPATKSTVKADPDEPSPSNPLASEAATPARARRTVKKKV

Target ORF information:

RefSeq Version	NM_165196.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165196.2

ORF Insert Sequence:

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AspRS-m ( NM_001273590.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260519.1 Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15480
Clone ID Related Accession (Same CDS sequence)	NM_165196.2 , NM_001273590.1 , NM_001273591.1
Accession Version	NM_001273590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Aspartyl-tRNA synthetase, mitochondrial, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260519.1
CDS	190..3438
Translation	MSGRYRGGGGGRFGGRGGGGYADGGGYNDFDNYRGGGGPGFNDNFGPGPGPNPFNMGGPNISGNELMQLMSAMASNFNMNSQPPQPMRQSCGELRNHHCGLFVELSGRLIKKRVNRFAELRDRNGGACQLVVLEDKHPRVARRMNNMPENTTLTIVGLVMRRPHNSCNQTMPTGEIEVEVQDILNIHFPAGGTKRAGDKRTYSTMVQQQSNLGITSTEYKIAKNENILKYFENRDLTCNDLRRDDVGKTVTLVGWIPSTKNNKFLQLKDGYGQTQLMIEDQSLSDTFLSTPEQTVIQIVGKVLGRPKANVNLKYDTGEVEVSVTSVKVLNPDDPYDGPIKAKEKKQKFSIDDLEAEEAAAEASANLTSNNGESTEDSAEASTTANASLIAEQRAKVADTNKFADRTHNCGELTSNDINEKVVICGWLEFQRMNKFFILRDAYGQTQVLLSPKTYGLEEYAETGVPIESIVRVEGTVIPRPAATINPKMQTGHVEVEADKVVVLNPAKKNLPFEIRKFNRAGERLRLTHRYLDLRFNDMQHNLRLRSAVIMKMREYLINYLGFVEVETPTLFRRTPGGAQEFVVPTRKAGHFYSLVQSPQQFKQMLMSGGIDRYFQVARCYRDEATRPDRQPEFTQLDIELSFTSRDDIMQLIEETLRYSWPKDFPRLQTPFRRITYEEAMEKYGNDKPDTRFGFLLNNVSEIIEKSDEFKEKYDDLGAYAIVVRASEAFWNGAARKHYESLGKEFKGTLFVRKFGPTKDVQEKLGKLLGEDVATEVADKFDLEENDLLFLGIGSKVETRELLGRIRLDYQDFLVENAKIKKPNDFRFLWVIDFPLFERNRETNQLESVHHPFTLPHSDDLENFATSCENLESIRSQAYDLVLNGQEVGGGSIRIHDRDMQHFILEQILKIPHDHLSHLLSALESGCPPHGGIALGLDRLIAILCRARSIRDVIAFPKSLNGRDPLSNAPVPISDEEMRLYHLSVVDEEEPSTNNTSHEQEDDDPDQERAPPSPMSATSESEQPQINVDIKQEPEGLNEDFAPESPATKSTVKADPDEPSPSNPLASEAATPARARRTVKKKV

Target ORF information:

RefSeq Version	NM_001273590.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273590.1

ORF Insert Sequence:

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AspRS-m ( NM_001273591.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260520.1 Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant C, mRNA.

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CloneID	OFa15480
Clone ID Related Accession (Same CDS sequence)	NM_165196.2 , NM_001273590.1 , NM_001273591.1
Accession Version	NM_001273591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Aspartyl-tRNA synthetase, mitochondrial, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260520.1
CDS	290..3538
Translation	MSGRYRGGGGGRFGGRGGGGYADGGGYNDFDNYRGGGGPGFNDNFGPGPGPNPFNMGGPNISGNELMQLMSAMASNFNMNSQPPQPMRQSCGELRNHHCGLFVELSGRLIKKRVNRFAELRDRNGGACQLVVLEDKHPRVARRMNNMPENTTLTIVGLVMRRPHNSCNQTMPTGEIEVEVQDILNIHFPAGGTKRAGDKRTYSTMVQQQSNLGITSTEYKIAKNENILKYFENRDLTCNDLRRDDVGKTVTLVGWIPSTKNNKFLQLKDGYGQTQLMIEDQSLSDTFLSTPEQTVIQIVGKVLGRPKANVNLKYDTGEVEVSVTSVKVLNPDDPYDGPIKAKEKKQKFSIDDLEAEEAAAEASANLTSNNGESTEDSAEASTTANASLIAEQRAKVADTNKFADRTHNCGELTSNDINEKVVICGWLEFQRMNKFFILRDAYGQTQVLLSPKTYGLEEYAETGVPIESIVRVEGTVIPRPAATINPKMQTGHVEVEADKVVVLNPAKKNLPFEIRKFNRAGERLRLTHRYLDLRFNDMQHNLRLRSAVIMKMREYLINYLGFVEVETPTLFRRTPGGAQEFVVPTRKAGHFYSLVQSPQQFKQMLMSGGIDRYFQVARCYRDEATRPDRQPEFTQLDIELSFTSRDDIMQLIEETLRYSWPKDFPRLQTPFRRITYEEAMEKYGNDKPDTRFGFLLNNVSEIIEKSDEFKEKYDDLGAYAIVVRASEAFWNGAARKHYESLGKEFKGTLFVRKFGPTKDVQEKLGKLLGEDVATEVADKFDLEENDLLFLGIGSKVETRELLGRIRLDYQDFLVENAKIKKPNDFRFLWVIDFPLFERNRETNQLESVHHPFTLPHSDDLENFATSCENLESIRSQAYDLVLNGQEVGGGSIRIHDRDMQHFILEQILKIPHDHLSHLLSALESGCPPHGGIALGLDRLIAILCRARSIRDVIAFPKSLNGRDPLSNAPVPISDEEMRLYHLSVVDEEEPSTNNTSHEQEDDDPDQERAPPSPMSATSESEQPQINVDIKQEPEGLNEDFAPESPATKSTVKADPDEPSPSNPLASEAATPARARRTVKKKV

Target ORF information:

RefSeq Version	NM_001273591.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Aspartyl-tRNA synthetase, mitochondrial (AspRS-m), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273591.1

ORF Insert Sequence:

ATGAGTGGCA GATATCGCGG TGGAGGCGGC GGTCGATTTG GTGGCCGTGG TGGCGGAGGC 
TATGCAGATG GTGGCGGCTA CAATGACTTC GACAACTACC GTGGTGGCGG AGGACCAGGC 
TTCAATGACA ACTTTGGACC AGGTCCCGGA CCAAATCCTT TCAACATGGG TGGTCCCAAC 
ATTTCTGGCA ATGAGTTAAT GCAGCTGATG AGTGCCATGG CCAGCAACTT CAATATGAAT 
TCACAGCCCC CGCAGCCAAT GCGCCAGAGT TGCGGCGAGT TGCGCAATCA CCATTGCGGA 
TTATTTGTTG AGCTGTCCGG CAGGCTCATT AAGAAGCGGG TGAATCGGTT TGCGGAGCTG 
CGCGACCGCA ATGGCGGTGC ATGCCAGCTT GTGGTTCTGG AAGACAAGCA TCCGCGAGTG 
GCACGGCGCA TGAACAACAT GCCAGAGAAC ACAACACTAA CTATTGTGGG CCTGGTCATG 
CGGCGACCAC ACAATTCGTG CAATCAAACA ATGCCAACGG GCGAGATCGA AGTGGAGGTG 
CAGGACATAC TTAACATTCA TTTCCCGGCA GGCGGTACCA AAAGAGCTGG TGACAAGCGC 
ACATACAGCA CAATGGTGCA GCAGCAGAGT AATCTTGGGA TTACCAGCAC CGAGTACAAG 
ATAGCCAAGA ACGAGAATAT ATTGAAGTAT TTTGAAAACC GTGATCTCAC CTGCAACGAT 
CTAAGGCGCG ACGATGTCGG AAAGACAGTG ACTTTGGTTG GTTGGATTCC CTCTACCAAA 
AACAACAAAT TCTTGCAGCT CAAAGATGGT TATGGCCAAA CGCAACTAAT GATCGAGGAT 
CAGTCGCTTA GCGACACATT TCTTTCAACA CCTGAGCAGA CAGTTATCCA AATAGTGGGC 
AAAGTTCTGG GGAGACCAAA AGCTAATGTT AATTTGAAAT ATGATACTGG TGAGGTGGAA 
GTCAGTGTAA CTAGCGTAAA AGTTCTCAAT CCGGACGATC CCTACGATGG TCCTATTAAG 
GCCAAAGAGA AGAAACAAAA GTTTTCCATT GATGATCTTG AGGCTGAGGA GGCTGCCGCC 
GAAGCATCCG CCAATCTTAC CAGCAATAAT GGAGAATCCA CGGAAGACAG CGCGGAAGCA 
AGTACCACTG CTAATGCCTC TTTAATTGCC GAGCAACGAG CCAAGGTGGC CGATACCAAC 
AAGTTTGCCG ACCGCACTCA TAACTGCGGG GAACTAACCT CCAATGATAT CAACGAAAAA 
GTAGTCATAT GCGGCTGGTT GGAGTTCCAG CGCATGAACA AATTCTTCAT ACTTCGGGAT 
GCTTATGGAC AAACTCAGGT ACTGTTGTCA CCGAAAACAT ATGGTTTGGA AGAATACGCA 
GAAACAGGCG TGCCCATTGA GTCAATTGTT CGAGTTGAGG GTACAGTGAT TCCCCGTCCA 
GCGGCTACAA TAAACCCCAA AATGCAAACT GGACATGTTG AGGTAGAAGC GGATAAAGTG 
GTTGTTCTCA ATCCGGCAAA GAAAAATCTT CCGTTTGAGA TCCGAAAGTT TAATCGTGCT 
GGCGAACGCT TGCGGCTTAC TCATCGGTAT CTGGATCTAC GATTTAATGA TATGCAGCAT 
AATCTGCGCC TTCGATCGGC TGTTATAATG AAAATGCGAG AGTATCTTAT AAACTATCTG 
GGTTTCGTCG AAGTGGAGAC ACCAACACTC TTCAGACGAA CGCCAGGCGG AGCTCAGGAG 
TTTGTAGTAC CCACTCGCAA AGCGGGCCAC TTTTATTCGC TCGTACAGAG TCCGCAGCAA 
TTCAAGCAGA TGCTGATGTC CGGTGGAATT GACAGATACT TCCAGGTGGC CCGTTGCTAC 
AGGGATGAAG CGACGCGTCC AGATCGTCAG CCGGAGTTCA CCCAGTTGGA TATTGAGCTA 
TCATTTACCA GCAGGGATGA TATCATGCAA CTGATTGAGG AGACTCTGCG TTATTCCTGG 
CCCAAAGATT TTCCGCGTCT GCAAACACCG TTCCGTCGTA TTACTTATGA GGAGGCAATG 
GAAAAGTACG GAAATGACAA GCCGGACACT CGGTTTGGTT TTCTGTTAAA CAACGTGTCG 
GAAATCATCG AAAAGAGCGA TGAATTCAAG GAAAAATACG ATGATCTTGG CGCTTATGCC 
ATTGTAGTGC GAGCCAGTGA AGCCTTTTGG AATGGAGCTG CCCGAAAGCA TTACGAAAGC 
CTAGGCAAGG AGTTCAAGGG TACGTTGTTC GTGCGCAAAT TTGGCCCAAC AAAGGATGTG 
CAGGAGAAAC TGGGAAAGCT ACTAGGCGAA GATGTAGCTA CAGAGGTCGC CGATAAGTTC 
GACCTTGAGG AGAACGATCT CCTTTTCTTG GGCATTGGAT CAAAAGTGGA AACTCGCGAA 
TTGTTGGGTC GCATCCGACT GGACTATCAA GACTTTCTGG TGGAAAATGC CAAAATAAAG 
AAGCCTAATG ACTTCCGCTT CCTTTGGGTG ATCGATTTCC CGTTGTTCGA GCGAAATCGT 
GAGACTAATC AGTTGGAGAG CGTTCACCAC CCGTTTACCC TGCCACATAG TGATGATCTA 
GAAAATTTCG CCACTAGCTG CGAAAACTTG GAGAGCATAC GCTCCCAGGC TTACGATTTG 
GTGCTGAACG GCCAGGAAGT CGGAGGCGGT TCAATTCGCA TCCATGATCG AGACATGCAG 
CACTTTATAT TGGAACAGAT TCTGAAAATC CCGCATGATC ATTTGTCGCA CTTACTGAGC 
GCATTGGAGT CTGGATGCCC GCCTCATGGA GGCATTGCTT TGGGCTTGGA TCGCTTGATT 
GCCATTTTAT GCCGTGCGCG ATCCATACGC GATGTGATTG CATTCCCCAA GTCACTCAAT 
GGGCGTGATC CCCTTTCAAA CGCACCAGTT CCCATTTCCG ATGAGGAGAT GAGGCTGTAC 
CACCTTTCTG TGGTAGATGA GGAAGAGCCC AGCACAAATA ACACGTCACA CGAACAGGAG 
GACGACGATC CCGATCAAGA ACGTGCTCCT CCATCCCCGA TGTCGGCGAC TAGCGAATCC 
GAGCAGCCGC AAATTAATGT AGATATTAAG CAGGAGCCCG AAGGTCTGAA TGAGGATTTT 
GCTCCTGAAT CCCCCGCAAC CAAATCGACA GTAAAAGCTG ATCCCGATGA ACCCTCACCA 
TCTAACCCCC TGGCATCGGA GGCTGCTACG CCGGCCAGAG CAAGAAGGAC TGTTAAAAAG 
AAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AspRS-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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