Dars2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dars2
Entrez Gene ID	226539
Full Name	aspartyl-tRNA synthetase 2 (mitochondrial)
Synonyms	5830468K18Rik,aspRS
General protein information	Preferred Names aspartyl-tRNA synthetase 2 (mitochondrial) Names aspartate--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 H2.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006496758.2	XP_006496821.1	aspartate--tRNA ligase, mitochondrial isoform X2
XM_006496758.3	XP_006496821.1	aspartate--tRNA ligase, mitochondrial isoform X2
NM_172644.3	NP_766232.1	aspartate--tRNA ligase, mitochondrial precursor
NM_172644.4	NP_766232.1	aspartate--tRNA ligase, mitochondrial precursor
XM_006496757.1	XP_006496820.1	aspartate--tRNA ligase, mitochondrial isoform X1
XM_006496757.2	XP_006496820.1	aspartate--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DARS2	XP_422251.3
Drosophila melanogaster (fruit fly)	CG31739	NP_001260519.1
Schizosaccharomyces pombe (fission yeast)	SPCC736.06	NP_587777.1
Xenopus tropicalis (tropical clawed frog)	dars2	XP_002931512.2
Homo sapiens (human)	DARS2	NP_060592.2
Pan troglodytes (chimpanzee)	DARS2	XP_524974.2
Kluyveromyces lactis	KLLA0E24465g	XP_455053.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007844	XP_317655.3
Neurospora crassa	NCU02585	XP_965716.2
Eremothecium gossypii	AGOS_AGR206C	NP_986872.1
Caenorhabditis elegans (roundworm)	dars-2	NP_506019.2
Macaca mulatta (Rhesus monkey)	DARS2	XP_001101931.2
Danio rerio (zebrafish)	dars2	XP_699804.3
Magnaporthe oryzae (rice blast fungus)	MGG_13512	XP_003716295.1
Canis lupus familiaris (dog)	DARS2	XP_547457.2
Oryza sativa (rice)	Os01g0153400	NP_001042054.1
Bos taurus (cattle)	DARS2	NP_001095692.1
Mus musculus (house mouse)	Dars2	NP_766232.1
Rattus norvegicus (Norway rat)	Dars2	NP_001029315.1
Saccharomyces cerevisiae (baker's yeast)	MSD1	NP_015221.1
Arabidopsis thaliana (thale cress)	AT4G33760	NP_195102.2

Gene Ontology
Bibliography

Related articles in PubMed

DARS2 protects against neuroinflammation and apoptotic neuronal loss, but is dispensable for myelin producing cells.
Aradjanski M, Dogan SA, Lotter S, Wang S, Hermans S, Wibom R, Rugarli E, Trifunovic A
Human molecular genetics26(21)4181-4189(2017 11)

In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL.
Fröhlich D, Suchowerska AK, Spencer ZH, von Jonquieres G, Klugmann CB, Bongers A, Delerue F, Stefen H, Ittner LM, Fath T, Housley GD, Klugmann M
Neurobiology of disease97(Pt A)24-35(2017 01)

Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.
Seiferling D, Szczepanowska K, Becker C, Senft K, Hermans S, Maiti P, König T, Kukat A, Trifunovic A
EMBO reports17(7)953-64(2016 07)

Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.
Dogan SA, Pujol C, Maiti P, Kukat A, Wang S, Hermans S, Senft K, Wibom R, Rugarli EI, Trifunovic A
Cell metabolism19(3)458-69(2014 Mar)

Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.
Scheper GC, van der Klok T, van Andel RJ, van Berkel CG, Sissler M, Smet J, Muravina TI, Serkov SV, Uziel G, Bugiani M, Schiffmann R, Krägeloh-Mann I, Smeitink JA, Florentz C, Van Coster R, Pronk JC, van der Knaap MS
Nature genetics39(4)534-9(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu28688	XM_006496757.2 Latest version!	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu28689	XM_006496758.3 Latest version!	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu18863	NM_172644.4 Latest version!	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OMu28689	XM_006496758.2	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu18863	NM_172644.3	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OMu28688	XM_006496757.1	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dars2 ( NM_172644.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766232.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

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CloneID	OMu18863
Clone ID Related Accession (Same CDS sequence)	NM_172644.3 , NM_172644.4
Accession Version	NM_172644.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	aspartate--tRNA ligase, mitochondrial precursor
Comment

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGAAATA CTTAAGGAAG 
GAAGACATTG AGTTCATTAG GAAGTTTGCA GTTCACCATT TCAGTCAGGA AGTCCTACCT 
ATATTCCTGA ATGCCAAGAA GAACTGGAGT TCGCCATTTG CTAAGTTCAT AATGGAGGAG 
GAAAGACTGG AATTAGCCAG ATCGATGGAG ATACAAGAAG AAGACATAGT GTTACTGACT 
GCTGGGGAAC ATGAGAAAGC ATGCTCTCTG TTGGGAAAGT TACGGCTAGA GTGTGCAGAC 
CTTTTGGAGA TGAGAGGAGC GGTGCTCCGA GACCCAGCAG TGTTCTCTTT TCTTTGGGTG 
GTTGACTTTC CACTCTTCCT TGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAT 
CACCCATTTA CTGCTCCTAA CTCCAGTGAC ATTCACCTCC TCTATACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTATGA CTTGGTTTTA AACGGCAATG AGATAGGAGG TGGTTCTGTC 
CGAATCCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CACTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA CGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTATGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAG GACCTTATGA GCAATGCTCC AGACTCCGTG 
TCTCCAGAAG AGCTGAAGCC TTACCATATC CACGTCTTGT GGCCAGCAGA CTCAGAAGAG 
GAGAGTGCAT CCGCCACACC TTCCAAACAC TTGAGCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_766232.1

Translation

MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	NM_172644.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172644.3

ORF Insert Sequence:

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGAAATA CTTAAGGAAG 
GAAGACATTG AGTTCATTAG GAAGTTTGCA GTTCACCATT TCAGTCAGGA AGTCCTACCT 
ATATTCCTGA ATGCCAAGAA GAACTGGAGT TCGCCATTTG CTAAGTTCAT AATGGAGGAG 
GAAAGACTGG AATTAGCCAG ATCGATGGAG ATACAAGAAG AAGACATAGT GTTACTGACT 
GCTGGGGAAC ATGAGAAAGC ATGCTCTCTG TTGGGAAAGT TACGGCTAGA GTGTGCAGAC 
CTTTTGGAGA TGAGAGGAGC GGTGCTCCGA GACCCAGCAG TGTTCTCTTT TCTTTGGGTG 
GTTGACTTTC CACTCTTCCT TGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAT 
CACCCATTTA CTGCTCCTAA CTCCAGTGAC ATTCACCTCC TCTATACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTATGA CTTGGTTTTA AACGGCAATG AGATAGGAGG TGGTTCTGTC 
CGAATCCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CACTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA CGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTATGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAG GACCTTATGA GCAATGCTCC AGACTCCGTG 
TCTCCAGAAG AGCTGAAGCC TTACCATATC CACGTCTTGT GGCCAGCAGA CTCAGAAGAG 
GAGAGTGCAT CCGCCACACC TTCCAAACAC TTGAGCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dars2 ( XM_006496757.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496820.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu28688
Clone ID Related Accession (Same CDS sequence)	XM_006496757.1 , XM_006496757.2
Accession Version	XM_006496757.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	aspartate--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGGAAGT CCTACCTATA 
TTCCTGAATG CCAAGAAGAA CTGGAGTTCG CCATTTGCTA AGTTCATAAT GGAGGAGGAA 
AGACTGGAAT TAGCCAGATC GATGGAGATA CAAGAAGAAG ACATAGTGTT ACTGACTGCT 
GGGGAACATG AGAAAGCATG CTCTCTGTTG GGAAAGTTAC GGCTAGAGTG TGCAGACCTT 
TTGGAGATGA GAGGAGCGGT GCTCCGAGAC CCAGCAGTGT TCTCTTTTCT TTGGGTGGTT 
GACTTTCCAC TCTTCCTTGC CAAGGAAGAG AGTCCCACAG AGCTGGAGTC AGCCCATCAC 
CCATTTACTG CTCCTAACTC CAGTGACATT CACCTCCTCT ATACTGAGCC GGAAAAGGTT 
CGTGGCCAAC ACTATGACTT GGTTTTAAAC GGCAATGAGA TAGGAGGTGG TTCTGTCCGA 
ATCCATGATG CACAGCTACA GAGGTACATC CTGGAAACAC TGCTAAAGGA AGATGTGAAA 
TTGCTTTCCC ATCTGCTTCA GGCTTTAGAC TATGGGGCAC CCCCACACGG AGGAATTGCC 
TTAGGGTTAG ACAGACTGGT ATGTCTTGTC ACGGGAGCAC CCAGCATCAG AGATGTCATA 
GCCTTCCCTA AATCCTACCG AGGACAGGAC CTTATGAGCA ATGCTCCAGA CTCCGTGTCT 
CCAGAAGAGC TGAAGCCTTA CCATATCCAC GTCTTGTGGC CAGCAGACTC AGAAGAGGAG 
AGTGCATCCG CCACACCTTC CAAACACTTG AGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496820.1
CDS	543..2441
Translation	MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	XM_006496757.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496757.1

ORF Insert Sequence:

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGGAAGT CCTACCTATA 
TTCCTGAATG CCAAGAAGAA CTGGAGTTCG CCATTTGCTA AGTTCATAAT GGAGGAGGAA 
AGACTGGAAT TAGCCAGATC GATGGAGATA CAAGAAGAAG ACATAGTGTT ACTGACTGCT 
GGGGAACATG AGAAAGCATG CTCTCTGTTG GGAAAGTTAC GGCTAGAGTG TGCAGACCTT 
TTGGAGATGA GAGGAGCGGT GCTCCGAGAC CCAGCAGTGT TCTCTTTTCT TTGGGTGGTT 
GACTTTCCAC TCTTCCTTGC CAAGGAAGAG AGTCCCACAG AGCTGGAGTC AGCCCATCAC 
CCATTTACTG CTCCTAACTC CAGTGACATT CACCTCCTCT ATACTGAGCC GGAAAAGGTT 
CGTGGCCAAC ACTATGACTT GGTTTTAAAC GGCAATGAGA TAGGAGGTGG TTCTGTCCGA 
ATCCATGATG CACAGCTACA GAGGTACATC CTGGAAACAC TGCTAAAGGA AGATGTGAAA 
TTGCTTTCCC ATCTGCTTCA GGCTTTAGAC TATGGGGCAC CCCCACACGG AGGAATTGCC 
TTAGGGTTAG ACAGACTGGT ATGTCTTGTC ACGGGAGCAC CCAGCATCAG AGATGTCATA 
GCCTTCCCTA AATCCTACCG AGGACAGGAC CTTATGAGCA ATGCTCCAGA CTCCGTGTCT 
CCAGAAGAGC TGAAGCCTTA CCATATCCAC GTCTTGTGGC CAGCAGACTC AGAAGAGGAG 
AGTGCATCCG CCACACCTTC CAAACACTTG AGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dars2 ( XM_006496757.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496820.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.

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CloneID	OMu28688
Clone ID Related Accession (Same CDS sequence)	XM_006496757.1 , XM_006496757.2
Accession Version	XM_006496757.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	aspartate--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496757.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGGAAGT CCTACCTATA 
TTCCTGAATG CCAAGAAGAA CTGGAGTTCG CCATTTGCTA AGTTCATAAT GGAGGAGGAA 
AGACTGGAAT TAGCCAGATC GATGGAGATA CAAGAAGAAG ACATAGTGTT ACTGACTGCT 
GGGGAACATG AGAAAGCATG CTCTCTGTTG GGAAAGTTAC GGCTAGAGTG TGCAGACCTT 
TTGGAGATGA GAGGAGCGGT GCTCCGAGAC CCAGCAGTGT TCTCTTTTCT TTGGGTGGTT 
GACTTTCCAC TCTTCCTTGC CAAGGAAGAG AGTCCCACAG AGCTGGAGTC AGCCCATCAC 
CCATTTACTG CTCCTAACTC CAGTGACATT CACCTCCTCT ATACTGAGCC GGAAAAGGTT 
CGTGGCCAAC ACTATGACTT GGTTTTAAAC GGCAATGAGA TAGGAGGTGG TTCTGTCCGA 
ATCCATGATG CACAGCTACA GAGGTACATC CTGGAAACAC TGCTAAAGGA AGATGTGAAA 
TTGCTTTCCC ATCTGCTTCA GGCTTTAGAC TATGGGGCAC CCCCACACGG AGGAATTGCC 
TTAGGGTTAG ACAGACTGGT ATGTCTTGTC ACGGGAGCAC CCAGCATCAG AGATGTCATA 
GCCTTCCCTA AATCCTACCG AGGACAGGAC CTTATGAGCA ATGCTCCAGA CTCCGTGTCT 
CCAGAAGAGC TGAAGCCTTA CCATATCCAC GTCTTGTGGC CAGCAGACTC AGAAGAGGAG 
AGTGCATCCG CCACACCTTC CAAACACTTG AGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496820.1
CDS	553..2451
Translation	MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	XM_006496757.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496757.2

ORF Insert Sequence:

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGGAAGT CCTACCTATA 
TTCCTGAATG CCAAGAAGAA CTGGAGTTCG CCATTTGCTA AGTTCATAAT GGAGGAGGAA 
AGACTGGAAT TAGCCAGATC GATGGAGATA CAAGAAGAAG ACATAGTGTT ACTGACTGCT 
GGGGAACATG AGAAAGCATG CTCTCTGTTG GGAAAGTTAC GGCTAGAGTG TGCAGACCTT 
TTGGAGATGA GAGGAGCGGT GCTCCGAGAC CCAGCAGTGT TCTCTTTTCT TTGGGTGGTT 
GACTTTCCAC TCTTCCTTGC CAAGGAAGAG AGTCCCACAG AGCTGGAGTC AGCCCATCAC 
CCATTTACTG CTCCTAACTC CAGTGACATT CACCTCCTCT ATACTGAGCC GGAAAAGGTT 
CGTGGCCAAC ACTATGACTT GGTTTTAAAC GGCAATGAGA TAGGAGGTGG TTCTGTCCGA 
ATCCATGATG CACAGCTACA GAGGTACATC CTGGAAACAC TGCTAAAGGA AGATGTGAAA 
TTGCTTTCCC ATCTGCTTCA GGCTTTAGAC TATGGGGCAC CCCCACACGG AGGAATTGCC 
TTAGGGTTAG ACAGACTGGT ATGTCTTGTC ACGGGAGCAC CCAGCATCAG AGATGTCATA 
GCCTTCCCTA AATCCTACCG AGGACAGGAC CTTATGAGCA ATGCTCCAGA CTCCGTGTCT 
CCAGAAGAGC TGAAGCCTTA CCATATCCAC GTCTTGTGGC CAGCAGACTC AGAAGAGGAG 
AGTGCATCCG CCACACCTTC CAAACACTTG AGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dars2 ( XM_006496758.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496821.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.

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CloneID	OMu28689
Clone ID Related Accession (Same CDS sequence)	XM_006496758.2 , XM_006496758.3
Accession Version	XM_006496758.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	aspartate--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006496758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACGG GTGAGATTGA AATCAAAGTT AAAACAGCTG AGCTCCTGAA TGCCTGCAAA 
AAGCTGCCCT TTGAGATTAA AGACTTTGTG AAGAAAACAG AGGCGCTCCG TTTGCAGTAT 
CGCTACTTAG ATCTGCGCAG CTTCCAGATG CAGTACAACT TGCGGCTGAG GTCGCAGATG 
GTCATGAAAA TGCGGGAATA TCTCTGTAAT CTGCACGGGT TTGTGGATAT AGAAACCCCA 
ACCTTGTTTA AGAGGACCCC TGGAGGTGCC AAGGAGTTTT TAGTACCATC CAGGGAACCT 
GGAAAGTTTT ATTCCCTCCC TCAAAGTCCT CAGCAATTTA AGCAGCTTCT GATGGTTGGA 
GGTTTAGACA GGTATTTTCA GGTCGCTCGG TGTTACCGAG ATGAAGGCTC AAGACCGGAT 
AGACAGCCAG AGTTCACTCA GATTGACATA GAGATGTCCT TTGTAGAGCA GACTGGCATT 
CAGCGTTTAG TTGAGGGTTT GCTGCAGTAT TCCTGGCCTG GTGATAAAGA CCCTCTAGTG 
ACTCCTTTCC CTTCCATGAC TTTTGCTGAG GCACTGGCCA CCTATGGAAC TGATAAGCCA 
GATACTCGCT TTGGGATGAA GATTGTGGAT GTCAGTGATG TGTTCAGAAA CACAGAGCTC 
AGATTCCTTC AGGATGCACT GGCTAAGCCC CAAGGAACTG TCAAAGCAAT ATGTGTTCAT 
GACGGAGCGA AATACTTAAG GAAGGAAGAC ATTGAGTTCA TTAGGAAGTT TGCAGTTCAC 
CATTTCAGTC AGGAAGTCCT ACCTATATTC CTGAATGCCA AGAAGAACTG GAGTTCGCCA 
TTTGCTAAGT TCATAATGGA GGAGGAAAGA CTGGAATTAG CCAGATCGAT GGAGATACAA 
GAAGAAGACA TAGTGTTACT GACTGCTGGG GAACATGAGA AAGCATGCTC TCTGTTGGGA 
AAGTTACGGC TAGAGTGTGC AGACCTTTTG GAGATGAGAG GAGCGGTGCT CCGAGACCCA 
GCAGTGTTCT CTTTTCTTTG GGTGGTTGAC TTTCCACTCT TCCTTGCCAA GGAAGAGAGT 
CCCACAGAGC TGGAGTCAGC CCATCACCCA TTTACTGCTC CTAACTCCAG TGACATTCAC 
CTCCTCTATA CTGAGCCGGA AAAGGTTCGT GGCCAACACT ATGACTTGGT TTTAAACGGC 
AATGAGATAG GAGGTGGTTC TGTCCGAATC CATGATGCAC AGCTACAGAG GTACATCCTG 
GAAACACTGC TAAAGGAAGA TGTGAAATTG CTTTCCCATC TGCTTCAGGC TTTAGACTAT 
GGGGCACCCC CACACGGAGG AATTGCCTTA GGGTTAGACA GACTGGTATG TCTTGTCACG 
GGAGCACCCA GCATCAGAGA TGTCATAGCC TTCCCTAAAT CCTACCGAGG ACAGGACCTT 
ATGAGCAATG CTCCAGACTC CGTGTCTCCA GAAGAGCTGA AGCCTTACCA TATCCACGTC 
TTGTGGCCAG CAGACTCAGA AGAGGAGAGT GCATCCGCCA CACCTTCCAA ACACTTGAGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496821.1
CDS	355..1920
Translation	MPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	XM_006496758.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496758.2

ORF Insert Sequence:

ATGCCGACGG GTGAGATTGA AATCAAAGTT AAAACAGCTG AGCTCCTGAA TGCCTGCAAA 
AAGCTGCCCT TTGAGATTAA AGACTTTGTG AAGAAAACAG AGGCGCTCCG TTTGCAGTAT 
CGCTACTTAG ATCTGCGCAG CTTCCAGATG CAGTACAACT TGCGGCTGAG GTCGCAGATG 
GTCATGAAAA TGCGGGAATA TCTCTGTAAT CTGCACGGGT TTGTGGATAT AGAAACCCCA 
ACCTTGTTTA AGAGGACCCC TGGAGGTGCC AAGGAGTTTT TAGTACCATC CAGGGAACCT 
GGAAAGTTTT ATTCCCTCCC TCAAAGTCCT CAGCAATTTA AGCAGCTTCT GATGGTTGGA 
GGTTTAGACA GGTATTTTCA GGTCGCTCGG TGTTACCGAG ATGAAGGCTC AAGACCGGAT 
AGACAGCCAG AGTTCACTCA GATTGACATA GAGATGTCCT TTGTAGAGCA GACTGGCATT 
CAGCGTTTAG TTGAGGGTTT GCTGCAGTAT TCCTGGCCTG GTGATAAAGA CCCTCTAGTG 
ACTCCTTTCC CTTCCATGAC TTTTGCTGAG GCACTGGCCA CCTATGGAAC TGATAAGCCA 
GATACTCGCT TTGGGATGAA GATTGTGGAT GTCAGTGATG TGTTCAGAAA CACAGAGCTC 
AGATTCCTTC AGGATGCACT GGCTAAGCCC CAAGGAACTG TCAAAGCAAT ATGTGTTCAT 
GACGGAGCGA AATACTTAAG GAAGGAAGAC ATTGAGTTCA TTAGGAAGTT TGCAGTTCAC 
CATTTCAGTC AGGAAGTCCT ACCTATATTC CTGAATGCCA AGAAGAACTG GAGTTCGCCA 
TTTGCTAAGT TCATAATGGA GGAGGAAAGA CTGGAATTAG CCAGATCGAT GGAGATACAA 
GAAGAAGACA TAGTGTTACT GACTGCTGGG GAACATGAGA AAGCATGCTC TCTGTTGGGA 
AAGTTACGGC TAGAGTGTGC AGACCTTTTG GAGATGAGAG GAGCGGTGCT CCGAGACCCA 
GCAGTGTTCT CTTTTCTTTG GGTGGTTGAC TTTCCACTCT TCCTTGCCAA GGAAGAGAGT 
CCCACAGAGC TGGAGTCAGC CCATCACCCA TTTACTGCTC CTAACTCCAG TGACATTCAC 
CTCCTCTATA CTGAGCCGGA AAAGGTTCGT GGCCAACACT ATGACTTGGT TTTAAACGGC 
AATGAGATAG GAGGTGGTTC TGTCCGAATC CATGATGCAC AGCTACAGAG GTACATCCTG 
GAAACACTGC TAAAGGAAGA TGTGAAATTG CTTTCCCATC TGCTTCAGGC TTTAGACTAT 
GGGGCACCCC CACACGGAGG AATTGCCTTA GGGTTAGACA GACTGGTATG TCTTGTCACG 
GGAGCACCCA GCATCAGAGA TGTCATAGCC TTCCCTAAAT CCTACCGAGG ACAGGACCTT 
ATGAGCAATG CTCCAGACTC CGTGTCTCCA GAAGAGCTGA AGCCTTACCA TATCCACGTC 
TTGTGGCCAG CAGACTCAGA AGAGGAGAGT GCATCCGCCA CACCTTCCAA ACACTTGAGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dars2 ( XM_006496758.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006496821.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.

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CloneID	OMu28689
Clone ID Related Accession (Same CDS sequence)	XM_006496758.2 , XM_006496758.3
Accession Version	XM_006496758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	aspartate--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACGG GTGAGATTGA AATCAAAGTT AAAACAGCTG AGCTCCTGAA TGCCTGCAAA 
AAGCTGCCCT TTGAGATTAA AGACTTTGTG AAGAAAACAG AGGCGCTCCG TTTGCAGTAT 
CGCTACTTAG ATCTGCGCAG CTTCCAGATG CAGTACAACT TGCGGCTGAG GTCGCAGATG 
GTCATGAAAA TGCGGGAATA TCTCTGTAAT CTGCACGGGT TTGTGGATAT AGAAACCCCA 
ACCTTGTTTA AGAGGACCCC TGGAGGTGCC AAGGAGTTTT TAGTACCATC CAGGGAACCT 
GGAAAGTTTT ATTCCCTCCC TCAAAGTCCT CAGCAATTTA AGCAGCTTCT GATGGTTGGA 
GGTTTAGACA GGTATTTTCA GGTCGCTCGG TGTTACCGAG ATGAAGGCTC AAGACCGGAT 
AGACAGCCAG AGTTCACTCA GATTGACATA GAGATGTCCT TTGTAGAGCA GACTGGCATT 
CAGCGTTTAG TTGAGGGTTT GCTGCAGTAT TCCTGGCCTG GTGATAAAGA CCCTCTAGTG 
ACTCCTTTCC CTTCCATGAC TTTTGCTGAG GCACTGGCCA CCTATGGAAC TGATAAGCCA 
GATACTCGCT TTGGGATGAA GATTGTGGAT GTCAGTGATG TGTTCAGAAA CACAGAGCTC 
AGATTCCTTC AGGATGCACT GGCTAAGCCC CAAGGAACTG TCAAAGCAAT ATGTGTTCAT 
GACGGAGCGA AATACTTAAG GAAGGAAGAC ATTGAGTTCA TTAGGAAGTT TGCAGTTCAC 
CATTTCAGTC AGGAAGTCCT ACCTATATTC CTGAATGCCA AGAAGAACTG GAGTTCGCCA 
TTTGCTAAGT TCATAATGGA GGAGGAAAGA CTGGAATTAG CCAGATCGAT GGAGATACAA 
GAAGAAGACA TAGTGTTACT GACTGCTGGG GAACATGAGA AAGCATGCTC TCTGTTGGGA 
AAGTTACGGC TAGAGTGTGC AGACCTTTTG GAGATGAGAG GAGCGGTGCT CCGAGACCCA 
GCAGTGTTCT CTTTTCTTTG GGTGGTTGAC TTTCCACTCT TCCTTGCCAA GGAAGAGAGT 
CCCACAGAGC TGGAGTCAGC CCATCACCCA TTTACTGCTC CTAACTCCAG TGACATTCAC 
CTCCTCTATA CTGAGCCGGA AAAGGTTCGT GGCCAACACT ATGACTTGGT TTTAAACGGC 
AATGAGATAG GAGGTGGTTC TGTCCGAATC CATGATGCAC AGCTACAGAG GTACATCCTG 
GAAACACTGC TAAAGGAAGA TGTGAAATTG CTTTCCCATC TGCTTCAGGC TTTAGACTAT 
GGGGCACCCC CACACGGAGG AATTGCCTTA GGGTTAGACA GACTGGTATG TCTTGTCACG 
GGAGCACCCA GCATCAGAGA TGTCATAGCC TTCCCTAAAT CCTACCGAGG ACAGGACCTT 
ATGAGCAATG CTCCAGACTC CGTGTCTCCA GAAGAGCTGA AGCCTTACCA TATCCACGTC 
TTGTGGCCAG CAGACTCAGA AGAGGAGAGT GCATCCGCCA CACCTTCCAA ACACTTGAGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006496821.1
CDS	1022..2587
Translation	MPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	XM_006496758.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006496758.3

ORF Insert Sequence:

ATGCCGACGG GTGAGATTGA AATCAAAGTT AAAACAGCTG AGCTCCTGAA TGCCTGCAAA 
AAGCTGCCCT TTGAGATTAA AGACTTTGTG AAGAAAACAG AGGCGCTCCG TTTGCAGTAT 
CGCTACTTAG ATCTGCGCAG CTTCCAGATG CAGTACAACT TGCGGCTGAG GTCGCAGATG 
GTCATGAAAA TGCGGGAATA TCTCTGTAAT CTGCACGGGT TTGTGGATAT AGAAACCCCA 
ACCTTGTTTA AGAGGACCCC TGGAGGTGCC AAGGAGTTTT TAGTACCATC CAGGGAACCT 
GGAAAGTTTT ATTCCCTCCC TCAAAGTCCT CAGCAATTTA AGCAGCTTCT GATGGTTGGA 
GGTTTAGACA GGTATTTTCA GGTCGCTCGG TGTTACCGAG ATGAAGGCTC AAGACCGGAT 
AGACAGCCAG AGTTCACTCA GATTGACATA GAGATGTCCT TTGTAGAGCA GACTGGCATT 
CAGCGTTTAG TTGAGGGTTT GCTGCAGTAT TCCTGGCCTG GTGATAAAGA CCCTCTAGTG 
ACTCCTTTCC CTTCCATGAC TTTTGCTGAG GCACTGGCCA CCTATGGAAC TGATAAGCCA 
GATACTCGCT TTGGGATGAA GATTGTGGAT GTCAGTGATG TGTTCAGAAA CACAGAGCTC 
AGATTCCTTC AGGATGCACT GGCTAAGCCC CAAGGAACTG TCAAAGCAAT ATGTGTTCAT 
GACGGAGCGA AATACTTAAG GAAGGAAGAC ATTGAGTTCA TTAGGAAGTT TGCAGTTCAC 
CATTTCAGTC AGGAAGTCCT ACCTATATTC CTGAATGCCA AGAAGAACTG GAGTTCGCCA 
TTTGCTAAGT TCATAATGGA GGAGGAAAGA CTGGAATTAG CCAGATCGAT GGAGATACAA 
GAAGAAGACA TAGTGTTACT GACTGCTGGG GAACATGAGA AAGCATGCTC TCTGTTGGGA 
AAGTTACGGC TAGAGTGTGC AGACCTTTTG GAGATGAGAG GAGCGGTGCT CCGAGACCCA 
GCAGTGTTCT CTTTTCTTTG GGTGGTTGAC TTTCCACTCT TCCTTGCCAA GGAAGAGAGT 
CCCACAGAGC TGGAGTCAGC CCATCACCCA TTTACTGCTC CTAACTCCAG TGACATTCAC 
CTCCTCTATA CTGAGCCGGA AAAGGTTCGT GGCCAACACT ATGACTTGGT TTTAAACGGC 
AATGAGATAG GAGGTGGTTC TGTCCGAATC CATGATGCAC AGCTACAGAG GTACATCCTG 
GAAACACTGC TAAAGGAAGA TGTGAAATTG CTTTCCCATC TGCTTCAGGC TTTAGACTAT 
GGGGCACCCC CACACGGAGG AATTGCCTTA GGGTTAGACA GACTGGTATG TCTTGTCACG 
GGAGCACCCA GCATCAGAGA TGTCATAGCC TTCCCTAAAT CCTACCGAGG ACAGGACCTT 
ATGAGCAATG CTCCAGACTC CGTGTCTCCA GAAGAGCTGA AGCCTTACCA TATCCACGTC 
TTGTGGCCAG CAGACTCAGA AGAGGAGAGT GCATCCGCCA CACCTTCCAA ACACTTGAGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dars2 ( NM_172644.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766232.1 Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

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CloneID	OMu18863
Clone ID Related Accession (Same CDS sequence)	NM_172644.3 , NM_172644.4
Accession Version	NM_172644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Mus musculus(house mouse)
Product	aspartate--tRNA ligase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY781897.1, AK138809.1 and AK030952.1. On Sep 1, 2015 this sequence version replaced NM_172644.3. ##Evidence-Data-START## Transcript exon combination :: AK030952.1, AK138809.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164140, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGAAATA CTTAAGGAAG 
GAAGACATTG AGTTCATTAG GAAGTTTGCA GTTCACCATT TCAGTCAGGA AGTCCTACCT 
ATATTCCTGA ATGCCAAGAA GAACTGGAGT TCGCCATTTG CTAAGTTCAT AATGGAGGAG 
GAAAGACTGG AATTAGCCAG ATCGATGGAG ATACAAGAAG AAGACATAGT GTTACTGACT 
GCTGGGGAAC ATGAGAAAGC ATGCTCTCTG TTGGGAAAGT TACGGCTAGA GTGTGCAGAC 
CTTTTGGAGA TGAGAGGAGC GGTGCTCCGA GACCCAGCAG TGTTCTCTTT TCTTTGGGTG 
GTTGACTTTC CACTCTTCCT TGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAT 
CACCCATTTA CTGCTCCTAA CTCCAGTGAC ATTCACCTCC TCTATACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTATGA CTTGGTTTTA AACGGCAATG AGATAGGAGG TGGTTCTGTC 
CGAATCCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CACTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA CGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTATGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAG GACCTTATGA GCAATGCTCC AGACTCCGTG 
TCTCCAGAAG AGCTGAAGCC TTACCATATC CACGTCTTGT GGCCAGCAGA CTCAGAAGAG 
GAGAGTGCAT CCGCCACACC TTCCAAACAC TTGAGCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766232.1
CDS	545..2506
Translation	MYLGFWLSRLCRGLSRPIGKTMRPIWGSLSRNLALSSQRIPEFSSFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASVRRILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGAKYLRKEDIEFIRKFAVHHFSQEVLPIFLNAKKNWSSPFAKFIMEEERLELARSMEIQEEDIVLLTAGEHEKACSLLGKLRLECADLLEMRGAVLRDPAVFSFLWVVDFPLFLAKEESPTELESAHHPFTAPNSSDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSVRIHDAQLQRYILETLLKEDVKLLSHLLQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGQDLMSNAPDSVSPEELKPYHIHVLWPADSEEESASATPSKHLSS

Target ORF information:

RefSeq Version	NM_172644.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172644.4

ORF Insert Sequence:

ATGTATCTTG GTTTTTGGTT AAGTCGTCTA TGCAGGGGTT TATCCAGACC CATCGGGAAG 
ACCATGCGGC CAATCTGGGG TTCTCTTTCC AGAAATCTGG CGTTGAGTTC ACAAAGAATT 
CCAGAATTCA GTAGCTTTGT TGCTCGGACC AACACATGTG GAGAGTTGCG TTCGTCCCAC 
TTAGGCCAAG AAGTCACTCT GTGTGGATGG ATTCAGTACC GCAGGCAGAA CACCTTCTTA 
GTCCTAAGAG ATTGCCACGG CCTTGTTCAG ATTCTCATTC CCCAGGATGA GTCAGCCGCA 
TCTGTGAGGA GGATTTTGTG TGAAGCCCCT GTGGAGTCTG TGGTGAGAGT ATCTGGGACA 
GTAATCTCCC GTCCTCCAGG ACAAGAGAAT CCGAAAATGC CGACGGGTGA GATTGAAATC 
AAAGTTAAAA CAGCTGAGCT CCTGAATGCC TGCAAAAAGC TGCCCTTTGA GATTAAAGAC 
TTTGTGAAGA AAACAGAGGC GCTCCGTTTG CAGTATCGCT ACTTAGATCT GCGCAGCTTC 
CAGATGCAGT ACAACTTGCG GCTGAGGTCG CAGATGGTCA TGAAAATGCG GGAATATCTC 
TGTAATCTGC ACGGGTTTGT GGATATAGAA ACCCCAACCT TGTTTAAGAG GACCCCTGGA 
GGTGCCAAGG AGTTTTTAGT ACCATCCAGG GAACCTGGAA AGTTTTATTC CCTCCCTCAA 
AGTCCTCAGC AATTTAAGCA GCTTCTGATG GTTGGAGGTT TAGACAGGTA TTTTCAGGTC 
GCTCGGTGTT ACCGAGATGA AGGCTCAAGA CCGGATAGAC AGCCAGAGTT CACTCAGATT 
GACATAGAGA TGTCCTTTGT AGAGCAGACT GGCATTCAGC GTTTAGTTGA GGGTTTGCTG 
CAGTATTCCT GGCCTGGTGA TAAAGACCCT CTAGTGACTC CTTTCCCTTC CATGACTTTT 
GCTGAGGCAC TGGCCACCTA TGGAACTGAT AAGCCAGATA CTCGCTTTGG GATGAAGATT 
GTGGATGTCA GTGATGTGTT CAGAAACACA GAGCTCAGAT TCCTTCAGGA TGCACTGGCT 
AAGCCCCAAG GAACTGTCAA AGCAATATGT GTTCATGACG GAGCGAAATA CTTAAGGAAG 
GAAGACATTG AGTTCATTAG GAAGTTTGCA GTTCACCATT TCAGTCAGGA AGTCCTACCT 
ATATTCCTGA ATGCCAAGAA GAACTGGAGT TCGCCATTTG CTAAGTTCAT AATGGAGGAG 
GAAAGACTGG AATTAGCCAG ATCGATGGAG ATACAAGAAG AAGACATAGT GTTACTGACT 
GCTGGGGAAC ATGAGAAAGC ATGCTCTCTG TTGGGAAAGT TACGGCTAGA GTGTGCAGAC 
CTTTTGGAGA TGAGAGGAGC GGTGCTCCGA GACCCAGCAG TGTTCTCTTT TCTTTGGGTG 
GTTGACTTTC CACTCTTCCT TGCCAAGGAA GAGAGTCCCA CAGAGCTGGA GTCAGCCCAT 
CACCCATTTA CTGCTCCTAA CTCCAGTGAC ATTCACCTCC TCTATACTGA GCCGGAAAAG 
GTTCGTGGCC AACACTATGA CTTGGTTTTA AACGGCAATG AGATAGGAGG TGGTTCTGTC 
CGAATCCATG ATGCACAGCT ACAGAGGTAC ATCCTGGAAA CACTGCTAAA GGAAGATGTG 
AAATTGCTTT CCCATCTGCT TCAGGCTTTA GACTATGGGG CACCCCCACA CGGAGGAATT 
GCCTTAGGGT TAGACAGACT GGTATGTCTT GTCACGGGAG CACCCAGCAT CAGAGATGTC 
ATAGCCTTCC CTAAATCCTA CCGAGGACAG GACCTTATGA GCAATGCTCC AGACTCCGTG 
TCTCCAGAAG AGCTGAAGCC TTACCATATC CACGTCTTGT GGCCAGCAGA CTCAGAAGAG 
GAGAGTGCAT CCGCCACACC TTCCAAACAC TTGAGCTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DARS2 protects against neuroinflammation and apoptotic neuronal loss, but is dispensable for myelin producing cells.
Human molecular genetics26(21)4181-4189(2017 11)
Aradjanski M,Dogan SA,Lotter S,Wang S,Hermans S,Wibom R,Rugarli E,Trifunovic A

In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL.
Neurobiology of disease97(Pt A)24-35(2017 01)
Fröhlich D,Suchowerska AK,Spencer ZH,von Jonquieres G,Klugmann CB,Bongers A,Delerue F,Stefen H,Ittner LM,Fath T,Housley GD,Klugmann M

Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.
EMBO reports17(7)953-64(2016 07)
Seiferling D,Szczepanowska K,Becker C,Senft K,Hermans S,Maiti P,König T,Kukat A,Trifunovic A

Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart.
Cell metabolism19(3)458-69(2014 Mar)
Dogan SA,Pujol C,Maiti P,Kukat A,Wang S,Hermans S,Senft K,Wibom R,Rugarli EI,Trifunovic A

Mitochondrial aspartyl-tRNA synthetase deficiency causes leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation.
Nature genetics39(4)534-9(2007 Apr)
Scheper GC,van der Klok T,van Andel RJ,van Berkel CG,Sissler M,Smet J,Muravina TI,Serkov SV,Uziel G,Bugiani M,Schiffmann R,Krägeloh-Mann I,Smeitink JA,Florentz C,Van Coster R,Pronk JC,van der Knaap MS

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Dars2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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